常用pGEX载体图谱
Rosetta系列的表达菌株可以提供T7 RNA聚合酶，它能表达PET系列载体上的外源基因。。。pGEX系列载体上的外源基因不需要T7 RNA聚合酶，普通的大肠杆菌经IPTG诱导即可表达
Tac启动子是一组由Lac和trp启动子人工构建的杂合启动子，受Lac阻遏蛋白的负调节，它的启动能力比Lac和trp都强。其中Tac 1是由Trp启动子的－35区加上一个合成的46 bp DNA片段(包括Pribnow 盒)和Lac操纵基因构成，Tac 12是由Trp的启动子-35区和Lac启动子的-10区，加上Lac操纵子中的操纵基因部分和SD序列融合而成
蛋白标签：
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is (in 1- and 3-letter codes, respectively): N-EQKLISEEDL-C, N-Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-C, where N stands for Amino-terminus and C stands for Carboxy terminus. The tag is approximately 1202 Daltons in atomic mass and has 10 amino acids.
It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
A monoclonal antibody against the myc epitope, named 9E10, is available from the
non-commercial Developmental Studies Hybridoma Bank
pGEX4T1载体基本信息
出品公司:GE
别名:pGEX-4T-1, pGEX4T1, pGEX 4T 1
质粒类型:大肠杆菌蛋白表达载体
表达水平:高拷贝
启动子:Tac
克隆方法:多克隆位点，限制性内切酶
载体大小:4969 bp
5' 测序引物及序列:pGEX5': GGGCTGGCAAGCCACGTTTGGTG 3' 测序引物及序列:pGEX3': CCGGGAGCTGCATGTGTCAGAGG 载体标签:N-GST
载体抗性:Ampicillin 氨苄
备注:复制子是pMB1
产品目录号:27-4580-01
稳定性:瞬时表达Transient
组成型:诱导表达
病毒/非病毒:非病毒
pGEX4T1载体质粒图谱和多克隆位点信息
原核生物DNA复制起始点，是DNA链上独特的具有起始DNA复制功能的碱基序列。大肠杆菌的复制起点包括OriC和OriH
Thrombin 凝血酶
pGEX4T1载体简介
pGEX4T1载体序列
LOCUS pGEX-4T-1 4969 bp DNA circular SYN
DEFINITION pGEX-4T-1
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
https://www.360docs.net/doc/643858588.html,/
COMMENT VNTAUTHORNAME|https://www.360docs.net/doc/643858588.html,| FEATURES Location/Qualifiers
source 1..4969
/organism="pGEX-4T-1"
/mol_type="other DNA"
promoter 184..212
/label="tac_promoter"
misc_feature 224..246
/label="M13_pUC_rev_primer"
gene 258..977
/label="GST (variant)"
/gene="GST (variant)"
CDS 258..977
/label="ORF frame 3"
misc_feature complement(1019..1041)
/label="pGEX_3_primer"
promoter 1307..1335
/label="AmpR_promoter"
gene 1377..2237
/label="Ampicillin"
/gene="Ampicillin"
CDS 1377..2237
/label="ORF frame 3"
rep_origin 2392..3011
/label="pBR322_origin"
misc_feature 3309..4400
/label="lacI"
CDS 3441..4400
/label="ORF frame 3"
promoter 4449..4478
/label="lac_promoter"
misc_feature 4492..4514
/label="M13_pUC_rev_primer"
promoter 4513..4531
/label="M13_reverse_primer"
CDS 4523..81
/label="ORF frame 2"
misc_feature 4540..4695
/label="lacZ_a"
promoter complement(4543..4559)
/label="M13_forward20_primer"
misc_feature complement(4552..4574)
/label="M13_pUC_fwd_primer"
pGEX4T2载体基本信息
出品公司:GE
别名:pGEX-4T-2, pGEX4T2, pGEX 4T 2
质粒类型:大肠杆菌蛋白表达载体
表达水平:高拷贝
启动子:Tac
克隆方法:多克隆位点，限制性内切酶
载体大小:4970 bp
5' 测序引物及序列:pGEX5': GGGCTGGCAAGCCACGTTTGGTG 3' 测序引物及序列:pGEX3': CCGGGAGCTGCATGTGTCAGAGG 载体标签:N-GST
载体抗性:Ampicillin 氨苄
备注:复制子是pMB1
产品目录号:27-4581-01
稳定性:瞬时表达Transient
组成型:诱导表达
病毒/非病毒:非病毒
pGEX4T2载体质粒图谱和多克隆位点信息
pGEX4T2载体简介
pGEX4T2载体序列
LOCUS pGEX-4T-2 4970 bp DNA circular SYN DEFINITION pGEX-4T-2
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
https://www.360docs.net/doc/643858588.html,/
COMMENT VNTAUTHORNAME|https://www.360docs.net/doc/643858588.html,|