试题集-免疫学试题A卷及答案

The final examination of medical immunology

for seven year students of Grade 2003(2005/12/6)

Paper A

题号一二三四五总分统分人得分

得分评卷人

I. Explain the following items (2 points for each, 18 points)

1. Lymphocyte recirculation

扣分2. CDR

扣分3. cytokine

扣分4. MHC restriction

扣分

5. PRR

扣分6. Immune tolerance

扣分7. ITAM

扣分**

扣分9. TI-Ag

扣分II. Choose all correct answers (1 point for each,15 points) 得分评卷人

No. 1 2 3 4 5 6 7 8 Answer

No. 9 10 11 12 13

Answer

1.Which of the following statement(s) about the spleen is(are)

true:

A. It filters antigens out of the blood.

B. Lymphatic vessels draining the tissue spaces enter the spleen

C. It contains germinal centers

D. It functions to remove old and defective red blood cells

** kinds of Ig expressed mainly on the surface of mature B cells?

A. mIgG

B. mIg A

C. mIgD

D.mIgM

** can function as C3 convertase in activation of complement system?

** B.C4b2b3b C.C3bBb D.C3bnBb ** the characteristics of cytokines, which of the following(s) is

(are) correct?

A. One cytokine can act on different cells and play multiple biological effects

B. The cytokine produced by one cell only acts on neighbor cells

C. Combined with corresponding receptor to play role

D. One cytokine can inhibit or enhance effects of other cytokine

** of the following molecules can stimulate T cells activation?

A. LPS

B. PWM

C. Con A

D. PHA

** are the functions of B cells?

A. produce Ab

B. secrete cytokines

C. kill target cells directly

D. present Ag

** of the cytokines are secreted by Th1 cells?

A. IL-4

B. IL-2

C. IL-10

D. IFN-γ

** of the diseases belong to type Ⅲhypersensitivity?

A. Transfusion reaction

B. Arthus reaction

C. Contact dermatitis

D. Rheumatoid arthritis

** of the following is(are) correct about the characteristics of memory cells?

A. **e from either B cell or T cell

B. Can respond to antigen at low consentrate effectivelly

C. Only exist in HI

D. React to a second invasion of an antigen more rapidly and stronger

** abilities do αβ+T cells gain after maturation?

A. self-reactive

B. self-tolerance

C. express functional TCR

D. self MHC restriction

** of the followings are correct statements about dendritic cells?

A. Can stimulate na?ve T cell proliferation

B. Express high level of MHC class II molecules

C. Express high level of MHC class I molecules

D. Mature dendritic cells with strong function of antigen-capture

** AICD, which statements are correct?

A. Can be mediated by FasL-Fas interaction

B. Is a kind of cell death of activated T cell

C. Play important roles in immunological regulation

D. IL-4 can promote AICD

13. The processes of antigen-activated B cells differentiation in germinal center include:

A. Ig Class switching

B. Somatic hypermutation and Ig affinity maturation

C. Differentiate into Bm

D. Apoptosis

** of the following factors affect the antigen immunogenecity?

A. molecule weight

B. foreigness

C. conformation

D. **position

15. About somatic hypermutation, which statements are correct?

A. Occurs during B cells maturation

B. Result in affinity maturation of antibodies

C. Mainly occurs in CDR region

D. Can occur during T cells maturation

Ⅲ．Answer the following questions briefly (5 point for each, 30 points)

得分评卷人

1.How can T helper cells play important roles in an antibody mediated response.

扣分** cells express the HLA II molecules? What are the functions of HLA II molecules on these cells?

扣分** we want to label the antibody with enzyme for ELISA experiment, what part of antibody does enzyme should bind to? Why?

扣分** do effector specific CTLs kill tumor cells? What are the characteristics for this process?

扣分5. How are endogenous antigens recognized by CD8+ T cells?

扣分6. What is Neonate hemolysis? Please describe its mechanism.

扣分IV. Answer following question (12 points)

得分评卷人

1. How the innate and adaptive immunity act in cooperative and inter-dependent ways to protect the host.?

扣分

The final examination of medical immunology

for seven year students of Grade 2003(2005/12/6)

Paper A

I. Explain the following items (2 points for each, 18 points)

** recirculation: is the process by which lymphocytes continuously move between sites throughout the body(1) through blood and lymphatic vessels, and it is critical for the initiation and effector phases of immune responses(1).

2. CDR: Complementarity-determining region (0.5)

the three HVR of L chain and H chain are brought together to form antigen-binding surface, so HVR is also called CDR(1)

3. cytokine: A group of(0.5) low molecule weight proteins(0.5) which are secreted by activated immunocytes or some matrix cells(0.5) and take effects by binding corresponding receptor on cells(0.5).

** restriction: In interaction of T cell and antigen-presenting cell(APC) or target cell(0.5) ,T cell not only recognizes specific antigen peptide, but also recognizes polymophic residue of MHC molecules(0.5).

5. Pattern recognition receptor(PRR): The receptor on macrophage(0.5) which can recognize and bind specific molecular structure on some pathogens , injured or apoptotic cells(0.5).

6. Immune tolerance

a specific nonreactivity 1.0

towards a given antigen 0.5

in other circumstance is able to induce cell-mediated or humoral immunity 0.5

7. ITAM

immunoreceptor tyrosine-based activation motif 0.5

transduct activation signal 1.0

composed of two tyrosine residues separated by around 18 amino

acid-----when antigen binds, the tyrosine become phosphorylated by the receptor associated tyrosine kinases to transduct activatory signal 0.5

**

cells that can capture（0.5）, process antigen （0.5）, and present the antigenic peptide/MHC class I or II molecules（0.5）to T cells （0.5）are called antigen-presenting cells. They are also called A cell (Accessory cell).

9. TI-Ag (thymus independent Ag) : Ag which can stimulate B cells to produce Ab(0.5) without the help of T cell and MФ(0.5).

II. Choose all correct answers (1 point for each, 15 points)

No. 1 2 3 4 5 6 7 8 Answer ACD CD AC ACD BCD ABD BD BD No. 9 10 11 12 13 14 15 Answer ABD BCD ABC ABC ABCD ABCD ABC

Ⅲ．Answer the following questions briefly (5 point for each, 30 points)

得分评卷人

1. How can T helper cells play important roles in an antibody mediated response.

(1) Express CD40L(0.5),combine with CD40 expressing on B cell(0.5),provide co-stimulation signal during the activation of B cell(1).

(2) Secrete IL-4,5,6(0.5) etc. to promote proliferation and differentiation of B cell(1)

(3) Secrete cytokines to promote Ig isotype switching(1.5).

** cells express the HLA II molecules? What are the functions of HLA II molecules on these cells?

cells express the HLA II molecules (2)

professional APC(macrophage, dentritic cell and B cell),

activated T cell,

thymus epithelial cell et al.

the functions of HLA II molecules (1 point each)

?Participate in controlling Th cell- APC recognition in immunoreaction

?Participate in processing and presenting of antigen

?Participate in inducing the differentiation and development of T cell in thymus

** we want to label the antibody with enzyme for ELISA experiment, what part of antibody does enzyme should bind to? Why?

Fc fragment(2),because:

Fab binds to Ag specifically(1)

Fc has no effect on Ag binding, so Fc can be labeled to detect Ag-Ab complex without effection on its Ag binding activity(2)

?** do effector specific CTLs kill tumor cells? What are the characteristics for this process?

Specific recognition and binding of target cell by CTL

?Lethal hit to target cell

?Perforin 1

?Granzyme 1

?Fas/FasL pathway—apoptosis 0.5

?Specific killing 1

?classⅠMHC molecule restriction 1

?Continuous killing of target cells in short time, and no injury of CTL 0.5

5. How are endogenous antigens recognized by CD8+ T cells?

◆By class I MHC pathway 1

◆Processing of endogenous Ag：proteosome -LMP2, LMP7，antigen

peptides(8-13aa) 1

◆Transporting of antigen peptide into ER：TAP(transporter associated

with antigen processing) 1

◆Peptide loading of MHC I molecules：ER，antigen peptide—class Ⅰ

**plexes 1

Presenting to CD8+T cells：TCR-peptide, CD8-α3 1

6. What is Neonate hemolysis? Please describe its mechanism. Definition: Maternal IgG specific for the fetal blood groups crosses the placenta and destroys the fetal red blood cells 1

Mechanism:

Mainly induced by Rh antigen. It is Type II hypersensitivity 0.5 Rh antigens on the surface of fetal red blood cells induce IgG antibody production within mother body---the first pregnancy. 1 IgG crosses the placenta and bind with Rh antigen to form Ag-Ab complex---the second pregnancy. 1

Cell lysis: Ag-Ab complex **plement by classic pathway. MAC 0.5

ADCC 0.5

Oposinization by C and Ab promotes macrophages function. 0.5

IV. Answer following question (12 points)

得分评卷人

1. How do the innate and adaptive immunity act in cooperative and inter-dependent ways to protect the host?

Innate immunity → adaptive immunity( 8 points)

(1)macrophage and DC participate in antigenic presentation and initiate adaptive immunity 3 points

(2)macrophage,NK,Complement etc. participate in effector phage of adaptive immunity 3 points

(3)macrophage and NK secrete cytokines regulate types of adaptive imunity 2 points

Adaptive immunity→Innate immunity(4 points)

(1)Ab-Ag complex situmulate activation of complement by classical

pathway 1 points

(2)Activated T cells secrete cytokines to participate in innate immunity

1 points

(3)Activated T cells secrete cytokines to activate NK cell 1 points

(4)Activated T cells secrete cytokines to activate macrophage 1 points