C反应蛋白ELISA试剂盒说明书

C反应蛋白ELISA试剂盒说明书

C反应蛋白ELISA试剂盒说明书

产品规格：96T/48T。

本产品只用于科研实验。

C反应蛋白ELISA试剂盒说明书Elisa试剂盒标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能

马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3 的样品,因NaN3 抑制辣根过氧化物酶的（HRP）活性。

Elisa kit:

3．1 samples were collected as soon as possible after the extraction, extraction by the relevant literature, as soon as possible after the extraction experiment. If you can not

4．Test immediately, can put the sample at -20 deg.c for preservation, but should avoid repeated freezing and thawing

5．2 could not detect the NaN3 containing samples, because NaN3 inhibited the activity of horseradish peroxidase (HRP).

C反应蛋白ELISA试剂盒说明书Calculation：

To the standard concentration as the abscissa, OD value as the ordinate, draw the standard curve on coordinate paper, according to the OD value of samples from the standard curve found corresponding concentration; multiplied by the dilution multiple; or with the standard concentration and the OD value calculated straight line regression equation of the standard curve, the sample OD value in the equation, calculate the sample concentration, multiplied by the dilution factor is the actual concentration of the sample.

Experimental principle:

This kit is used to measure the level of the chorionic gonadotropin in the sample of small and medium rats by double antibody sandwich method. With purified mouse chorionic gonadotropin? Antibody coated microtiter plate, made of solid phase antibody, to package is the micropore of monoclonal antibody in turn added to collagenase, and then with HRP labeled collagenase? Antibody binding, forming antibody, antigen and enzyme labeled antibody complexes, after thorough washing, TMB substrate added color. TMB under the catalysis of the HRP enzyme into blue, and under the action of acid into the final yellow. The depth of color and the collagenase in the sample were positively correlated. Enzyme standard instrument in 450 nm wavelength was used to measure the absorbance (OD) and the standard curve calculation of a small sample in chorionic gonadotropin concentrations.

C反应蛋白ELISA试剂盒说明书

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C反应蛋白ELISA试剂盒说明书Matters needing attention：

1 kit from the cold storage environment should be removed at room temperature for 15-30 minutes after the use of the enzyme labeled package is not used up after the plate, the plate should be stored in a sealed bag.

2 washing buffer will crystallization, heated the water solubilization dilution, washing does not affect the results.

3 each step sample should be used, and often to check its accuracy, in order to avoid the test error. A sample within 5 mins, ifthenumberofsampleismuch, recommend to use volley like.

4 please make the standard curve at the same time, it is better to do the hole. Such as samples to be measured matter content is too high (the sample od high standard product the first hole hole OD), please first sample dilution multiples (n times) were measured and calculated please then multiplied by the total dilution ratio (n * * 5).

5 closure plate membrane only a one-time use, to avoid cross contamination.

6 substrate please avoid light preservation.

7 in strict accordance with the instructions of the operation, the results of the test must be determined in accordance with the enzyme standard instrument readings.

8 all samples, washing liquid and all kinds of waste should be treated as infectious agents.

9 different batches of this reagent can not be mixed.

10 if there is a difference between the specification and the English specification. C反应蛋白ELISA试剂盒说明书

友情提醒：公司经营的产品均为科研实验用，不可用于临床应用。