Botanical Research 植物学研究, 2018, 7(2), 113-119 Published Online March 2018 in Hans. http://www.360docs.net/doc/info-0796355fa88271fe910ef12d2af90242a895ab85.html /journal/br https://http://www.360docs.net/doc/info-0796355fa88271fe910ef12d2af90242a895ab85.html /10.12677/br.2018.72015

文章引用: 张园, 林春, 陈秋静, 刘正杰. 彩色马铃薯StMyb-like 基因克隆表达分析与干涉载体构建[J]. 植物学研究, 2018, 7(2): 113-119. DOI: 10.12677/br.2018.72015

Cloning and Expression Analysis of StMyb-like Gene of Color Potato and Construction of Interference Vector

Yuan Zhang 1,2, Chun Lin 1,2, Qiujing Chen 1, Zhengjie Liu 1,2*

1College of Agriculture and Biotechnology, Yunnan Agricultural University (YNAU), Kunming Yunnan 2Institute of Improvement and Utilization of Characteristic Resource Plants, Yunnan Agricultural University (YNAU), Kunming Yunnan

Received: Feb. 21st , 2018; accepted: Mar. 6th , 2018; published: Mar. 13th , 2018

Abstract Color potato is rich in anthocyanins, which is one of the natural antioxidants. In this study, one gene related to anthocyanin synthesis was cloned from the color potato “Black King Kong”, and this gene encoding protein is highly homologous to transcription factor R2R3-MYB, and named as StMyb-like . The whole length of this gene is 798 bp, which encodes a 265 amino acid, with a mole-cular weight of 30.23 kD. The expression of StMyb-like gene was analyzed by semi quantitative RT-PCR. It was found that the expression level of the gene in roots and stems was very low, but the expression level of anthocyanin in sweet potato, potato skin, buds and leaves was relatively high. In order to study the function of StMyb-like gene in color potato, the pART-MybRNAi interference vector was constructed by using DNA recombination technology, which provides a basis for fur-ther study of the possible life activities of the gene. Keywords

StMyb-like , Anthocyanin, Expression, RNAi, Color Potato

彩色马铃薯StMyb-like 基因克隆表达分析

与干涉载体构建

张 园1,2,林 春1,2,陈秋静1,刘正杰1,2*

1

云南农业大学农学与生物技术学院,云南 昆明 2云南农业大学,特色资源植物改良与利用研究所,云南 昆明

*通讯作者。