PNU-74654_LCMS_23477_MedChemExpress

File ..HEM32\1\DATA\20170209\BIZ\BIZ2017-209-WJ5.D Tgt Mass (EZX): 320.10 Injection Date : 9 Feb 17 2:04 pm +0800 Seq. Line : 0 Sample Name : BIZ2017-209-WJ5 Location : P2-F-05 Acq. Operator : GZZ_718 Inj : 1 Spec. Reported : MS Integration Inj Volume : 1 ul Acq. Method : D:\Chem32\1\METHODS\1-POS-3MIN.M

Analysis Method : D:\CHEM32\1\METHODS\1-POS-3MIN-PROSRSS.M

Catalog No : HY-101130 Batch#23477 Easy-Access Method: '1-POS-3MIN' 320.10 Method Info : Mobile Phase: A: water(0.01%TFA) B:ACN(0.01%TFA) Gradient: 5% to 95%B within 1.3 min Flow Rate :1.8ml/min

Column :SunFire C18, 4.6*50mm,3.5um A-RP-186 Oven Temperature : 45C

min*

0.5

1

1.5

2 2.5mAU 0

5001000

*DAD1 B, Sig=214,4 Ref=off 1.541

1.680

1.906

2.260

min*

0.51

1.5

2 2.5mAU 0

100200

*DAD1 C, Sig=254,4 Ref=off 1.353

1.678

1.906

2.260

min

0.51 1.5

2 2.50

100000

200000300000400000 MSD1 TIC, MS File ES-API, Pos, Scan, Frag: 70

1.903

min

0.5

1

1.5

2 2.50

100000

200000 Ion 321.1, MSD1 321.1,Target Mass 320.1 +H Positive, EIC=320.8:321.8

1.9011.920

Integration Results for DAD1 B, Sig=214,4 Ref=off

RetTim Width Area Height Area% MS(+)

|-------|--------|--------------|--------------|--------|------| 1.54 0.03 3.17 1.70 0.12 141 1.68 0.03 1.20 0.71 0.04 141 1.91 0.03 2682.34 1390.62 99.60 321 2.26 0.02 6.34 4.66 0.24 233

Integration Results for DAD1 C, Sig=254,4 Ref=off

RetTim Width Area Height Area% MS(+)|-------|--------|--------------|--------------|--------|------| 1.35 0.04 2.15 0.80 0.39 ND 1.68 0.05 2.40 0.76 0.44 233 1.91 0.03 543.00 278.76 98.67 321 2.26 0.02 2.76 1.98 0.50 141

Ret. Time: 1.90 <<<< POSITIVE SPECTRA >>>>

m/z

200

300

400

500

600

700

800

900

20000

400006000080000100000 ES-API Positive

380.3

322.1 664.3

321.1