DMEM,1640,IMDM成分对照表
DMEM
Concentration (mg/L)mM Glycine 300.4 L-Alanine
L-Arginine
L-Arginine hydrochloride840.398 L-Asparagine
L-Asparagine (freebase)
L-Aspartic acid
L-Cystine 630.201 L-Cystine 2HCl
L-Glutamic Acid
L-Glutamine 5844 L-Histidine
L-Histidine hydrochloride-H2O 420.2 L-Hydroxyproline
L-Isoleucine 1050.802 L-Leucine1050.802 L-Lysine hydrochloride 1460.798 L-Methionine300.201 L-Phenylalanine 660.4 L-Proline
L-Serine 420.4 L-Threonine 950.798 L-Tryptophan 160.0784 L-Tyrosine disodium salt
L-Tyrosine disodium salt dihydrate 1040.398 L-Valine940.803 Vitamins
Biotin
Choline chloride 40.0286 D-Calcium pantothenate40.00839 Folic Acid 40.00907 Niacinamide40.0328 Para-Aminobenzoic Acid
Pyridoxine hydrochloride40.0196 Riboflavin 0.40.00106 Thiamine hydrochloride 40.0119 Vitamin B12
i-Inositol7.20.04 Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 200 1.8 Calcium nitrate (Ca(NO3)2 4H2O)
Ferric Nitrate (Fe(NO3)3"9H2O)0.10.000248
Magnesium Sulfate (MgSO4) (anhyd.)97.670.814 Potassium Chloride (KCl) 400 5.33 Potassium Nitrate (KNO3)
Sodium Bicarbonate (NaHCO3) 370044.05 Sodium Chloride (NaCl)6400110.34 Sodium Phosphate monobasic (NaH2PO4-H2O) 1250.906 Sodium Phosphate dibasic (Na2HPO4) anhydrous
Sodium Selenite (Na2SeO3-5H20)
Other Components(±)
D-Glucose (Dextrose) high glucose450025 D-Glucose (Dextrose) low glucose 1000 5.56 Glutathione (reduced)
HEPES595825.03 Phenol Red 150.0399 Sodium Pyruvate1101
RPMI 1640IMDM
Concentration (mg/L)mM Concentration (mg/L)mM
100.133300.4
250.281
200 1.15
840.398
500.379
250.167
200.15300.226
650.20891.40.381
200.136750.51
300 2.055844
150.0968
420.2
200.153
500.3821050.802
500.3821050.802
400.2191460.798
150.101300.201
150.0909660.4
200.174400.348
300.286420.4
200.168950.798
50.0245160.0784
1040.462
290.111
200.171940.803
0.20.000820.0130.0000533
30.021440.0286
0.250.00052440.00839
10.0022740.00907
10.008240.0328
10.0073
10.0048540.0196
0.20.0005310.40.00106
10.0029740.0119
0.0050.000037
350.1947.20.04
165 1.49
1000.424
48.840.40797.670.814
400 5.33330 4.4
0.0760.000752 200023.81302436 6000103.45450077.59
1250.906 800 5.63
0.01730.0000658 200011.11450025
10.00326
595825.03595825.03
50.0133150.0399
1101