pcambia1304植物表达载体

pcambia1304

编号 载体名称

北京华越洋生物VECT0240 pcambia1304

pcambia1304载体基本信息

出品公司: Cambia

载体名称: pcambia1304

质粒类型: 植物双元表达载体

高拷贝/低拷贝: --‐--‐

启动子: --‐--‐

克隆方法: 多克隆位点，限制性内切酶

载体大小: 12361 b p 5' 测序引物及序列: --‐--‐

3' 测序引物及序列: --‐--‐

载体标签: --‐--‐

载体抗性: Kanamycin 筛选标记: --‐--‐

备注: --‐--‐

产品目录号: --‐--‐

稳定性: --‐--‐

组成型: --‐--‐

病毒/非病毒: --‐--‐

载体质粒图谱和多克隆位点信息

载体序列

LOCUS AF234300 12361 b p DNA circular S YN DEFINITION Binary v ector p CAMBIA--‐1304, c omplete s equence. ACCESSION AF234300

VERSION AF234300.1 GI:7638083

KEYWORDS .

SOURCE Binary v ector p CAMBIA--‐1304

ORGANISM Binary v ector p CAMBIA--‐1304

other s equences; a rtificial s equences; v ectors.

REFERENCE 1 (sites)

AUTHORS Hajdukiewicz,P., S vab,Z. a nd M aliga,P.

TITLE The s mall, v ersatile p PZP f amily o f A grobacterium b inary v ectors

for p lant t ransformation

JOURNAL Plant M ol. B iol. 25 (6), 989--‐994 (1994)

PUBMED 7919218

REFERENCE 2 (bases 1 t o 12361)

AUTHORS Roberts,C., R ajagopal,S., S mith,L.M., N guyen,T.A., Y ang,W.,

Nugrohu,S., R avi,K.S., V ijayachandra,K., H arcourt,R.L.,

Dransfield,L., D esamero,N., S lamet,I., H adjukiewicz,P., S vab,Z.,

Maliga,P., M ayer,J.E., K eese,P.K., K ilian,A. a nd J efferson,R.A.

TITLE A c omprehensive s et o f m odular v ectors f or a dvanced manipulations

and e fficient t ransformation o f p lants

JOURNAL Unpublished

REMARK Full d escription o f c onstructs

REFERENCE 3 (bases 1 t o 12361)

AUTHORS Roberts,C., R ajagopal,S., S mith,L.M., N guyen,T.A., Y ang,W.,

Nugrohu,S., R avi,K.S., V ijayachandra,K., H arcourt,R.L.,

Dransfield,L., D esamero,N., S lamet,I., H adjukiewicz,P., S vab,Z.,

Maliga,P., M ayer,J.E., K eese,P.K., K ilian,A. a nd J efferson,R.A.

TITLE Direct S ubmission

JOURNAL Submitted (15--‐FEB--‐2000) C AMBIA, C lunies R oss S t, B lack Mountain /

GPO B ox 3200, C anberra, A CT 2601, A ustralia

FEATURES Location/Qualifiers

source 1..12361

/organism="Binary v ector p CAMBIA--‐1304"

/mol_type="other D NA"

/db_xref="taxon:118392"

CDS 2..2569

/note="mgfp5*--‐gusA (N358Q) f usion

(2--‐730/731--‐2569)"

/codon_start=1

/product="mGFP5--‐GUSA f usion p rotein"

/protein_id="AAF65352.1"

/db_xref="GI:7638085"

/translation="MVDLTSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYG

KLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTI FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADK QKNGIKANFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE K

RDHMVLLEFVTAAGITHGMDELYKASLRPVETPTREIKKLDGLWAFSLDRENCGIDQ R

WWESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDA V

THYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGM VI

TDENGKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQ VV

ANGDVSVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDI Y

PLRVGIRSVAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIG A

NSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVN GETQQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPT R

PITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKL H

QPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFAD FA

TSQGILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQASHHHHHH V"

misc_feature complement(1802..1804)

/note="N358Q g lycosylation m utation"

misc_feature 2598..2850

/note="nos (nopaline s ynthase) 3'UTR (polyA s ignal)" misc_feature 2888..2913

/note="right b order T--‐DNA r epeat"

misc_feature complement(3954..4954)

/note="STA r egion f rom p VS1 p lasmid"

rep_origin complement(5547..6547)

/note="pVS1--‐REP; r eplication o rigin f rom p VS1"

misc_feature complement(6957..7217)

/note="bom s ite f rom p BR322"

rep_origin complement(7357..7637)

/note="pBR322 o rigin o f r eplication"

CDS complement(7928..8722)

/note="aadA (kanamycin r esistance) g ene a mplified from

pIG121Hm"

/codon_start=1

/product="aminoglycoside p hosphotransferase"

/protein_id="AAF65353.1"

/db_xref="GI:7638086"

/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK

MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCS EEY

EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"

misc_feature 9147..9172

/note="left b order r epeat f rom C58 T--‐DNA"

misc_feature 9239..9447

/note="CaMV 3'UTR (polyA s ignal)"

CDS complement(9463..10488)

/note="hptII (hygromycin r esistance) g ene"

/codon_start=1

/product="hygromycin p hosphotransferase"

/protein_id="AAF65354.1"

/db_xref="GI:7638087"

/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR

GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVID WS

EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLY Q

SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRR PS

TRPRAKK"

promoter complement(10524..11331)

/note="CaMV35S2; C aMV 35S p romoter, d uplicated"

promoter 11452..11506

/note="PlacZ; l acZ p romoter"

CDS 11534..11767

/codon_start=1

/product="LacZ a lpha f ragment"

/protein_id="AAF65351.1"

/db_xref="GI:7638084"

/translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"

misc_feature 11576..11626

/note="pUC18 M CS; p olylinker"

promoter 11818..12355

/note="CaMV35S; 35S p romoter f rom C aMV" ORIGIN

1 c atggtagat c tgactagta a aggagaaga a cttttcact g gagttgtcc c aattcttgt

61 t gaattagat g gtgatgtta a tgggcacaa a ttttctgtc a gtggagagg g tgaaggtga 121 t gcaacatac g gaaaactta c ccttaaatt t atttgcact a ctggaaaac t acctgttcc

181 g tggccaaca c ttgtcacta c tttctctta t ggtgttcaa t gcttttcaa g atacccaga

241 t catatgaag c ggcacgact t cttcaagag c gccatgcct g agggatacg t gcaggagag 301 g accatcttc t tcaaggacg a cgggaacta c aagacacgt g ctgaagtca a gtttgaggg 361 a gacaccctc g tcaacagga t cgagcttaa g ggaatcgat t tcaaggagg a cggaaacat 421 c ctcggccac a agttggaat a caactacaa c tcccacaac g tatacatca t ggccgacaa 481 g caaaagaac g gcatcaaag c caacttcaa g acccgccac a acatcgaag a cggcggcgt 541 g caactcgct g atcattatc a acaaaatac t ccaattggc g atggccctg t ccttttacc

601 a gacaaccat t acctgtcca c acaatctgc c ctttcgaaa g atcccaacg a aaagagaga 661 c cacatggtc c ttcttgagt t tgtaacagc t gctgggatt a cacatggca t ggatgaact

721 a tacaaagct a gtttacgtc c tgtagaaac c ccaacccgt g aaatcaaaa a actcgacgg 781 c ctgtgggca t tcagtctgg a tcgcgaaaa c tgtggaatt g atcagcgtt g gtgggaaag 841 c gcgttacaa g aaagccggg c aattgctgt g ccaggcagt t ttaacgatc a gttcgccga 901 t gcagatatt c gtaattatg c gggcaacgt c tggtatcag c gcgaagtct t tataccgaa

961 a ggttgggca g gccagcgta t cgtgctgcg t ttcgatgcg g tcactcatt a cggcaaagt 1021 g tgggtcaat a atcaggaag t gatggagca t cagggcggc t atacgccat t tgaagccga 1081 t gtcacgccg t atgttattg c cgggaaaag t gtacgtatc a ccgtttgtg t gaacaacga

1141 a ctgaactgg c agactatcc c gccgggaat g gtgattacc g acgaaaacg g caagaaaaa 1201 g cagtcttac t tccatgatt t ctttaacta t gccggaatc c atcgcagcg t aatgctcta

1261 c accacgccg a acacctggg t ggacgatat c accgtggtg a cgcatgtcg c gcaagactg 1321 t aaccacgcg t ctgttgact g gcaggtggt g gccaatggt g atgtcagcg t tgaactgcg 1381 t gatgcggat c aacaggtgg t tgcaactgg a caaggcact a gcgggactt t gcaagtggt 1441 g aatccgcac c tctggcaac c gggtgaagg t tatctctat g aactgtgcg t cacagccaa 1501 a agccagaca g agtgtgata t ctacccgct t cgcgtcggc a tccggtcag t ggcagtgaa 1561 g ggccaacag t tcctgatta a ccacaaacc g ttctacttt a ctggctttg g tcgtcatga

1621 a gatgcggac t tacgtggca a aggattcga t aacgtgctg a tggtgcacg a ccacgcatt 1681 a atggactgg a ttggggcca a ctcctaccg t acctcgcat t acccttacg c tgaagagat 1741 g ctcgactgg g cagatgaac a tggcatcgt g gtgattgat g aaactgctg c tgtcggctt 1801 t cagctgtct t taggcattg g tttcgaagc g ggcaacaag c cgaaagaac t gtacagcga 1861 a gaggcagtc a acggggaaa c tcagcaagc g cacttacag g cgattaaag a gctgatagc 1921 g cgtgacaaa a accacccaa g cgtggtgat g tggagtatt g ccaacgaac c ggatacccg 1981 t ccgcaaggt g cacgggaat a tttcgcgcc a ctggcggaa g caacgcgta a actcgaccc 2041 g acgcgtccg a tcacctgcg t caatgtaat g ttctgcgac g ctcacaccg a taccatcag 2101 c gatctcttt g atgtgctgt g cctgaaccg t tattacgga t ggtatgtcc a aagcggcga

2161 t ttggaaacg g cagagaagg t actggaaaa a gaacttctg g cctggcagg a gaaactgca 2221 t cagccgatt a tcatcaccg a atacggcgt g gatacgtta g ccgggctgc a ctcaatgta

2281 c accgacatg t ggagtgaag a gtatcagtg t gcatggctg g atatgtatc a ccgcgtctt

2341 t gatcgcgtc a gcgccgtcg t cggtgaaca g gtatggaat t tcgccgatt t tgcgacctc

2401 g caaggcata t tgcgcgttg g cggtaacaa g aaagggatc t tcactcgcg a ccgcaaacc 2461 g aagtcggcg g cttttctgc t gcaaaaacg c tggactggc a tgaacttcg g tgaaaaacc 2521 g cagcaggga g gcaaacaag c tagccacca c caccaccac c acgtgtgaa t tggtgacca 2581 g ctcgaattt c cccgatcgt t caaacattt g gcaataaag t ttcttaaga t tgaatcctg

2641 t tgccggtct t gcgatgatt a tcatataat t tctgttgaa t tacgttaag c atgtaataa

2701 t taacatgta a tgcatgacg t tatttatga g atgggtttt t atgattaga g tcccgcaat

2761 t atacattta a tacgcgata g aaaacaaaa t atagcgcgc a aactaggat a aattatcgc 2821 g cgcggtgtc a tctatgtta c tagatcggg a attaaacta t cagtgtttg a caggatata

2881 t tggcgggta a acctaagag a aaagagcgt t tattagaat a acggatatt t aaaagggcg 2941 t gaaaaggtt t atccgttcg t ccatttgta t gtgcatgcc a accacaggg t tcccctcgg

3001 g atcaaagta c tttgatcca a cccctccgc t gctatagtg c agtcggctt c tgacgttca

3061 g tgcagccgt c ttctgaaaa c gacatgtcg c acaagtcct a agttacgcg a caggctgcc

3121 g ccctgccct t ttcctggcg t tttcttgtc g cgtgtttta g tcgcataaa g tagaatact

3181 t gcgactaga a ccggagaca t tacgccatg a acaagagcg c cgccgctgg c ctgctgggc 3241 t atgcccgcg t cagcaccga c gaccaggac t tgaccaacc a acgggccga a ctgcacgcg 3301 g ccggctgca c caagctgtt t tccgagaag a tcaccggca c caggcgcga c cgcccggag 3361 c tggccagga t gcttgacca c ctacgccct g gcgacgttg t gacagtgac c aggctagac 3421 c gcctggccc g cagcacccg c gacctactg g acattgccg a gcgcatcca g gaggccggc 3481 g cgggcctgc g tagcctggc a gagccgtgg g ccgacacca c cacgccggc c ggccgcatg 3541 g tgttgaccg t gttcgccgg c attgccgag t tcgagcgtt c cctaatcat c gaccgcacc

3601 c ggagcgggc g cgaggccgc c aaggcccga g gcgtgaagt t tggcccccg c cctaccctc 3661 a ccccggcac a gatcgcgca c gcccgcgag c tgatcgacc a ggaaggccg c accgtgaaa 3721 g aggcggctg c actgcttgg c gtgcatcgc t cgaccctgt a ccgcgcact t gagcgcagc 3781 g aggaagtga c gcccaccga g gccaggcgg c gcggtgcct t ccgtgagga c gcattgacc 3841 g aggccgacg c cctggcggc c gccgagaat g aacgccaag a ggaacaagc a tgaaaccgc 3901 a ccaggacgg c caggacgaa c cgtttttca t taccgaaga g atcgaggcg g agatgatcg 3961 c ggccgggta c gtgttcgag c cgcccgcgc a cgtctcaac c gtgcggctg c atgaaatcc 4021 t ggccggttt g tctgatgcc a agctggcgg c ctggccggc c agcttggcc g ctgaagaaa 4081 c cgagcgccg c cgtctaaaa a ggtgatgtg t atttgagta a aacagcttg c gtcatgcgg

4141 t cgctgcgta t atgatgcga t gagtaaata a acaaatacg c aaggggaac g catgaaggt 4201 t atcgctgta c ttaaccaga a aggcgggtc a ggcaagacg a ccatcgcaa c ccatctagc 4261 c cgcgccctg c aactcgccg g ggccgatgt t ctgttagtc g attccgatc c ccagggcag

4321 t gcccgcgat t gggcggccg t gcgggaaga t caaccgcta a ccgttgtcg g catcgaccg 4381 c ccgacgatt g accgcgacg t gaaggccat c ggccggcgc g acttcgtag t gatcgacgg 4441 a gcgccccag g cggcggact t ggctgtgtc c gcgatcaag g cagccgact t cgtgctgat 4501 t ccggtgcag c caagccctt a cgacatatg g gccaccgcc g acctggtgg a gctggttaa 4561 g cagcgcatt g aggtcacgg a tggaaggct a caagcggcc t ttgtcgtgt c gcgggcgat 4621 c aaaggcacg c gcatcggcg g tgaggttgc c gaggcgctg g ccgggtacg a gctgcccat 4681 t cttgagtcc c gtatcacgc a gcgcgtgag c tacccaggc a ctgccgccg c cggcacaac 4741 c gttcttgaa t cagaacccg a gggcgacgc t gcccgcgag g tccaggcgc t ggccgctga

4801 a attaaatca a aactcattt g agttaatga g gtaaagaga a aatgagcaa a agcacaaac 4861 a cgctaagtg c cggccgtcc g agcgcacgc a gcagcaagg c tgcaacgtt g gccagcctg 4921 g cagacacgc c agccatgaa g cgggtcaac t ttcagttgc c ggcggagga t cacaccaag 4981 c tgaagatgt a cgcggtacg c caaggcaag a ccattaccg a gctgctatc t gaatacatc 5041 g cgcagctac c agagtaaat g agcaaatga a taaatgagt a gatgaattt t agcggctaa 5101 a ggaggcggc a tggaaaatc a agaacaacc a ggcaccgac g ccgtggaat g ccccatgtg 5161 t ggaggaacg g gcggttggc c aggcgtaag c ggctgggtt g tctgccggc c ctgcaatgg 5221 c actggaacc c ccaagcccg a ggaatcggc g tgacggtcg c aaaccatcc g gcccggtac 5281 a aatcggcgc g gcgctgggt g atgacctgg t ggagaagtt g aaggccgcg c aggccgccc 5341 a gcggcaacg c atcgaggca g aagcacgcc c cggtgaatc g tggcaagcg g ccgctgatc 5401 g aatccgcaa a gaatcccgg c aaccgccgg c agccggtgc g ccgtcgatt a ggaagccgc 5461 c caagggcga c gagcaacca g attttttcg t tccgatgct c tatgacgtg g gcacccgcg 5521 a tagtcgcag c atcatggac g tggccgttt t ccgtctgtc g aagcgtgac c gacgagctg 5581 g cgaggtgat c cgctacgag c ttccagacg g gcacgtaga g gtttccgca g ggccggccg 5641 g catggccag t gtgtgggat t acgacctgg t actgatggc g gtttcccat c taaccgaat

5701 c catgaaccg a taccgggaa g ggaagggag a caagcccgg c cgcgtgttc c gtccacacg 5761 t tgcggacgt a ctcaagttc t gccggcgag c cgatggcgg a aagcagaaa g acgacctgg 5821 t agaaacctg c attcggtta a acaccacgc a cgttgccat g cagcgtacg a agaaggcca 5881 a gaacggccg c ctggtgacg g tatccgagg g tgaagcctt g attagccgc t acaagatcg 5941 t aaagagcga a accgggcgg c cggagtaca t cgagatcga g ctagctgat t ggatgtacc 6001 g cgagatcac a gaaggcaag a acccggacg t gctgacggt t caccccgat t actttttga 6061 t cgatcccgg c atcggccgt t ttctctacc g cctggcacg c cgcgccgca g gcaaggcag 6121 a agccagatg g ttgttcaag a cgatctacg a acgcagtgg c agcgccgga g agttcaaga 6181 a gttctgttt c accgtgcgc a agctgatcg g gtcaaatga c ctgccggag t acgatttga

6241 a ggaggaggc g gggcaggct g gcccgatcc t agtcatgcg c taccgcaac c tgatcgagg 6301 g cgaagcatc c gccggttcc t aatgtacgg a gcagatgct a gggcaaatt g ccctagcag 6361 g ggaaaaagg t cgaaaaggt c tctttcctg t ggatagcac g tacattggg a acccaaagc 6421 c gtacattgg g aaccggaac c cgtacattg g gaacccaaa g ccgtacatt g ggaaccggt 6481 c acacatgta a gtgactgat a taaaagaga a aaaaggcga t ttttccgcc t aaaactctt 6541 t aaaacttat t aaaactctt a aaacccgcc t ggcctgtgc a taactgtct g gccagcgca

6601 c agccgaaga g ctgcaaaaa g cgcctaccc t tcggtcgct g cgctcccta c gccccgccg 6661 c ttcgcgtcg g cctatcgcg g ccgctggcc g ctcaaaaat g gctggccta c ggccaggca 6721 a tctaccagg g cgcggacaa g ccgcgccgt c gccactcga c cgccggcgc c cacatcaag 6781 g caccctgcc t cgcgcgttt c ggtgatgac g gtgaaaacc t ctgacacat g cagctcccg 6841 g agacggtca c agcttgtct g taagcggat g ccgggagca g acaagcccg t cagggcgcg 6901 t cagcgggtg t tggcgggtg t cggggcgca g ccatgaccc a gtcacgtag c gatagcgga 6961 g tgtatactg g cttaactat g cggcatcag a gcagattgt a ctgagagtg c accatatgc

7021 g gtgtgaaat a ccgcacaga t gcgtaagga g aaaataccg c atcaggcgc t cttccgctt 7081 c ctcgctcac t gactcgctg c gctcggtcg t tcggctgcg g cgagcggta t cagctcact

7141 c aaaggcggt a atacggtta t ccacagaat c aggggataa c gcaggaaag a acatgtgag 7201 c aaaaggcca g caaaaggcc a ggaaccgta a aaaggccgc g ttgctggcg t ttttccata 7261 g gctccgccc c cctgacgag c atcacaaaa a tcgacgctc a agtcagagg t ggcgaaacc 7321 c gacaggact a taaagatac c aggcgtttc c ccctggaag c tccctcgtg c gctctcctg 7381 t tccgaccct g ccgcttacc g gatacctgt c cgcctttct c ccttcggga a gcgtggcgc

7441 t ttctcatag c tcacgctgt a ggtatctca g ttcggtgta g gtcgttcgc t ccaagctgg

7501 g ctgtgtgca c gaacccccc g ttcagcccg a ccgctgcgc c ttatccggt a actatcgtc 7561 t tgagtccaa c ccggtaaga c acgacttat c gccactggc a gcagccact g gtaacagga 7621 t tagcagagc g aggtatgta g gcggtgcta c agagttctt g aagtggtgg c ctaactacg 7681 g ctacactag a aggacagta t ttggtatct g cgctctgct g aagccagtt a ccttcggaa 7741 a aagagttgg t agctcttga t ccggcaaac a aaccaccgc t ggtagcggt g gtttttttg 7801 t ttgcaagca g cagattacg c gcagaaaaa a aggatctca a gaagatcct t tgatctttt 7861 c tacggggtc t gacgctcag t ggaacgaaa a ctcacgtta a gggattttg g tcatgcatt 7921 c taggtacta a aacaattca t ccagtaaaa t ataatattt t attttctcc c aatcaggct

7981 t gatccccag t aagtcaaaa a atagctcga c atactgttc t tccccgata t cctccctga 8041 t cgaccggac g cagaaggca a tgtcatacc a cttgtccgc c ctgccgctt c tcccaagat 8101 c aataaagcc a cttactttg c catctttca c aaagatgtt g ctgtctccc a ggtcgccgt

8161 g ggaaaagac a agttcctct t cgggctttt c cgtctttaa a aaatcatac a gctcgcgcg 8221 g atctttaaa t ggagtgtct t cttcccagt t ttcgcaatc c acatcggcc a gatcgttat

8281 t cagtaagta a tccaattcg g ctaagcggc t gtctaagct a ttcgtatag g gacaatccg 8341 a tatgtcgat g gagtgaaag a gcctgatgc a ctccgcata c agctcgata a tcttttcag 8401 g gctttgttc a tcttcatac t cttccgagc a aaggacgcc a tcggcctca c tcatgagca 8461 g attgctcca g ccatcatgc c gttcaaagt g caggacctt t ggaacaggc a gctttcctt 8521 c cagccatag c atcatgtcc t tttcccgtt c cacatcata g gtggtccct t tataccggc

8581 t gtccgtcat t tttaaatat a ggttttcat t ttctcccac c agcttatat a ccttagcag

8641 g agacattcc t tccgtatct t ttacgcagc g gtatttttc g atcagtttt t tcaattccg

8701 g tgatattct c attttagcc a tttattatt t ccttcctct t ttctacagt a tttaaagat

8761 a ccccaagaa g ctaattata a caagacgaa c tccaattca c tgttccttg c attctaaaa 8821 c cttaaatac c agaaaacag c tttttcaaa g ttgttttca a agttggcgt a taacatagt 8881 a tcgacggag c cgattttga a accgcggtg a tcacaggca g caacgctct g tcatcgtta 8941 c aatcaacat g ctaccctcc g cgagatcat c cgtgtttca a acccggcag c ttagttgcc 9001 g ttcttccga a tagcatcgg t aacatgagc a aagtctgcc g ccttacaac g gctctcccg 9061 c tgacgccgt c ccggactga t gggctgcct g tatcgagtg g tgattttgt g ccgagctgc 9121 c ggtcgggga g ctgttggct g gctggtggc a ggatatatt g tggtgtaaa c aaattgacg 9181 c ttagacaac t taataacac a ttgcggacg t ttttaatgt a ctgaattaa c gccgaatta 9241 a ttcggggga t ctggatttt a gtactggat t ttggtttta g gaattagaa a ttttattga

9301 t agaagtatt t tacaaatac a aatacatac t aagggtttc t tatatgctc a acacatgag 9361 c gaaacccta t aggaaccct a attccctta t ctgggaact a ctcacacat t attatggag 9421 a aactcgagc t tgtcgatcg a cagatccgg t cggcatcta c tctatttct t tgccctcgg

9481 a cgagtgctg g ggcgtcggt t tccactatc g gcgagtact t ctacacagc c atcggtcca 9541 g acggccgcg c ttctgcggg c gatttgtgt a cgcccgaca g tcccggctc c ggatcggac 9601 g attgcgtcg c atcgaccct g cgcccaagc t gcatcatcg a aattgccgt c aaccaagct 9661 c tgatagagt t ggtcaagac c aatgcggag c atatacgcc c ggagtcgtg g cgatcctgc 9721 a agctccgga t gcctccgct c gaagtagcg c gtctgctgc t ccatacaag c caaccacgg 9781 c ctccagaag a agatgttgg c gacctcgta t tgggaatcc c cgaacatcg c ctcgctcca 9841 g tcaatgacc g ctgttatgc g gccattgtc c gtcaggaca t tgttggagc c gaaatccgc 9901 g tgcacgagg t gccggactt c ggggcagtc c tcggcccaa a gcatcagct c atcgagagc 9961 c tgcgcgacg g acgcactga c ggtgtcgtc c atcacagtt t gccagtgat a cacatgggg 10021 a tcagcaatc g cgcatatga a atcacgcca t gtagtgtat t gaccgattc c ttgcggtcc

10081 g aatgggccg a acccgctcg t ctggctaag a tcggccgca g cgatcgcat c catagcctc 10141 c gcgaccggt t gtagaacag c gggcagttc g gtttcaggc a ggtcttgca a cgtgacacc 10201 c tgtgcacgg c gggagatgc a ataggtcag g ctctcgcta a actccccaa t gtcaagcac 10261 t tccggaatc g ggagcgcgg c cgatgcaaa g tgccgataa a cataacgat c tttgtagaa 10321 a ccatcggcg c agctattta c ccgcaggac a tatccacgc c ctcctacat c gaagctgaa 10381 a gcacgagat t cttcgccct c cgagagctg c atcaggtcg g agacgctgt c gaacttttc 10441 g atcagaaac t tctcgacag a cgtcgcggt g agttcaggc t ttttcatat c tcattgccc

10501 c ccgggatct g cgaaagctc g agagagata g atttgtaga g agagactgg t gatttcagc 10561 g tgtcctctc c aaatgaaat g aacttcctt a tatagagga a ggtcttgcg a aggatagtg 10621 g gattgtgcg t catccctta c gtcagtgga g atatcacat c aatccactt g ctttgaaga 10681 c gtggttgga a cgtcttctt t ttccacgat g ctcctcgtg g gtgggggtc c atctttggg

10741 a ccactgtcg g cagaggcat c ttgaacgat a gcctttcct t tatcgcaat g atggcattt

10801 g taggtgcca c cttcctttt c tactgtcct t ttgatgaag t gacagatag c tgggcaatg

10861 g aatccgagg a ggtttcccg a tattaccct t tgttgaaaa g tctcaatag c cctttggtc

10921 t tctgagact g tatctttga t attcttgga g tagacgaga g tgtcgtgct c caccatgtt

10981 a tcacatcaa t ccacttgct t tgaagacgt g gttggaacg t cttcttttt c cacgatgct

11041 c ctcgtgggt g ggggtccat c tttgggacc a ctgtcggca g aggcatctt g aacgatagc 11101 c tttccttta t cgcaatgat g gcatttgta g gtgccacct t ccttttcta c tgtcctttt

11161 g atgaagtga c agatagctg g gcaatggaa t ccgaggagg t ttcccgata t taccctttg 11221 t tgaaaagtc t caatagccc t ttggtcttc t gagactgta t ctttgatat t cttggagta

11281 g acgagagtg t cgtgctcca c catgttggc a agctgctct a gccaatacg c aaaccgcct 11341 c tccccgcgc g ttggccgat t cattaatgc a gctggcacg a caggtttcc c gactggaaa 11401 g cgggcagtg a gcgcaacgc a attaatgtg a gttagctca c tcattaggc a ccccaggct 11461 t tacacttta t gcttccggc t cgtatgttg t gtggaattg t gagcggata a caatttcac

11521 a caggaaaca g ctatgacca t gattacgaa t tcgagctcg g tacccgggg a tcctctaga 11581 g tcgacctgc a ggcatgcaa g cttggcact g gccgtcgtt t tacaacgtc g tgactggga 11641 a aaccctggc g ttacccaac t taatcgcct t gcagcacat c cccctttcg c cagctggcg 11701 t aatagcgaa g aggcccgca c cgatcgccc t tcccaacag t tgcgcagcc t gaatggcga 11761 a tgctagagc a gcttgagct t ggatcagat t gtcgtttcc c gccttcagt t tagcttcat

11821 g gagtcaaag a ttcaaatag a ggacctaac a gaactcgcc g taaagactg g cgaacagtt 11881 c atacagagt c tcttacgac t caatgacaa g aagaaaatc t tcgtcaaca t ggtggagca 11941 c gacacactt g tctactcca a aaatatcaa a gatacagtc t cagaagacc a aagggcaat 12001 t gagactttt c aacaaaggg t aatatccgg a aacctcctc g gattccatt g cccagctat 12061 c tgtcacttt a ttgtgaaga t agtggaaaa g gaaggtggc t cctacaaat g ccatcattg 12121 c gataaagga a aggccatcg t tgaagatgc c tctgccgac a gtggtccca a agatggacc 12181 c ccacccacg a ggagcatcg t ggaaaaaga a gacgttcca a ccacgtctt c aaagcaagt 12241 g gattgatgt g atatctcca c tgacgtaag g gatgacgca c aatcccact a tccttcgca 12301 a gacccttcc t ctatataag g aagttcatt t catttggag a gaacacggg g gactcttga 12361 c

//

其他植物载体质粒：

pBI101 pDF15

pBI121 pEarleyGate 100

pBI221 pEarleyGate 101 pBI221--‐GFP pEarleyGate 102 pBin19 pEarleyGate 103 pBINPLUS pEarleyGate 104 pCambia0105.1R pEarleyGate 201 pCambia0305.1 pEarleyGate 202 pCambia0305.2 pEarleyGate 203 pCambia0380 pEarleyGate 204 pCambia0390 pEarleyGate 205 pCambia1105.1 pEarleyGate 301 pCambia1105.1R pEarleyGate 302 pCambia1200 pEarleyGate 303 pCambia1201 pEarleyGate 304 pCambia1281Z pFGC5941 pCambia1291Z pGA643 pCambia1300 pGreen pCambia1300GFP pGreen 0029 pCambia1301 pGreen0029 pCambia1302 pGreen029 pCambia1303 pGreenII pCambia1304 pGreenII 0049 pCambia1305.1 pGreenII 0179 pCambia1305.2 pGreenII 0229 pCambia1380 pGreenII 0579 pCambia1381 pHANNIBAL pCambia1381Xa pHELLSGATE pCambia1381Xb pHELLSGATE 12 pCambia1381Xc pHELLSGATE 4 pCambia1381Z pHELLSGATE 8 pCambia1390 pKANNIBAL pCambia1391 pPZP100 pCambia1391Xa pPZP101 pCambia1391Xb pPZP102 pCambia1391Xc pPZP111 pCambia1391Z pPZP112 pCambia2200 pPZP121 pCambia2201 pPZP122 pCambia2300 pPZP200 pCambia2301 pPZP201 pCambia2301--‐101 pPZP202 pCambia3200 pPZP211 pCambia3201 pPZP212 pCambia3300 pPZP221 pCambia3301 pPZP222

pCambia35s--‐ECFP pPZp--‐RCS2--‐Bar pCambia35s--‐EGFP pRI 101--‐AN pCambia35s--‐EYFP pRI 101--‐ON pCambia5105 pRI 201--‐AN pSB1 pRI 201--‐ON pSB11 pRI 909 pSoup pRI 910 pSPYCE(MR) pRI101

pTCK303 pSAT1--‐cCFP--‐C Super1300 pSAT1--‐cCFP--‐N pSAT6nCeruleanC(A+) pSAT4--‐nVenus--‐C