5s-23sRAN基因间隔区RELP分析对莱姆病病例尿标本的检测

5s-23sRAN基因间隔区RELP分析对莱姆病病例尿标本的

检测

史翠霞;张哲夫;万康林

【期刊名称】《中国人兽共患病学报》

【年(卷),期】2000(016)002

【摘要】作者对来自牡丹江市林业医院和海林林场的34份尿液标本进行了5s-23srRNA基因间隔区RFLP分析，并将PCR检测和血清学检测的结果进行比较。结果表明：8个病人PCR检测结果阳性，7个病人感染了B.garinii(第二基因种)、1个病人感染了B.afzelii(第三基因种)，同当地媒介生物中的优势基因种相同；血清学和PCR检测在对病人进行诊断时有互相弥补的作用。5s-23srRNA基因间隔区RFLP分析方法有望在研究临床表现和基因种的关系及流行病学调查中得到广泛应用。%The author tested 34 urine samples with 5s-23srRNA gene spacer RFLP analysis and compared PCR results with serodiagnosis results.8 pafients of urine are positive by PCR.

7 patients were infected by B. garinii, 1 patient by B afzelii. These genospecies infecting patients are identical with those infecting local vectors. The results showed that the accuracy of laboratory diagnosis oould be improved by simultaneously testing samples with PCR and serodiagnosis. The method of 5s-23srRNA gene spacer RFLP analysis will probably be used widely in studying the relationship of clinical manifestation and genospecies and in epidemiology investigations.【总页数】3页(21-23)