电生理膜片钳实验操作方法探讨

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电生理膜片钳实验操作方法探讨

作者：张永宁

来源：《科教导刊》2018年第03期

摘要膜片钳技术是生命科学研究中的重要技术手段，利用膜片钳技术可以准确地记录到细胞膜上离子通道的电活动和囊泡的分泌特性。在神经科学方面，膜片钳技术有助于阐明神经元内部蛋白分子的生理活动和功能，探究相关疾病的分子机制并提供潜在的治疗方法。但是，膜片钳实验的操作却有一定难度，初学者需要经过系统学习和长时间练习才能很好地掌握操作方法。本文以最常见的电压钳全细胞模式为例，结合膜片钳的具体操作过程，陈述了实验操作中应该特别注意的一些细节和问题，以协助实验人员记录到有效的实验数据。

关键词膜片钳技术操作方法全细胞模式

中图分类号：G642 文献标识码：A DOI：10.16400/https://www.360docs.net/doc/b54329470.html,ki.kjdkx.2018.01.020

Discussion on Experimental Operation Methods of

Electrophysical Patch-clamp

ZHANG Yongning

（College of Biomedical Engineering， South-central University for Nationalities， Wuhan，Hubei 430074）

Abstract Patch-clamp technique plays an important role in life science research， which can accurately record the electrical activity of ion channels on cell membrane and vesicle secretion characteristics. In neuroscience， patch-clamp technique helps to elucidate the physiological activity and function of protein molecules in neurons， explore the molecular mechanism of related diseases and provide potential therapeutic methods. However， the operation of patch-clamp experiment is difficult and beginners need to learn and practice for a long time to master the operation method. In this paper， the voltage-clamp whole-cell model were presented as an example， and according to the specific operation process of patch-clamp， some details and problems which should be paid special attention to in the experimental operations were stated， and this can help experimental operators get effective experimental data.

Keywords patch-clamp technique； operation methods； whole-cell model

膜片钳技术是物理学和生物学的交叉学科，自其问世以来对细胞生物学和电生理学产生了巨大的推动作用，尤其是在神经科学方面，基于膜片钳技术，对癫痫、神经病理性疼痛、神经退行性疾病（如阿尔兹海默症）等的电生理和分子机制的研究上取得重大进展，与这些疾病相