退变腰椎间盘中细胞凋亡及DR4、DcR1的表达

龙源期刊网 https://www.360docs.net/doc/cf11468467.html,

作者：何文徐杰

来源：《中外医学研究》2011年第15期

【摘要】目的检测死亡受体DR4及诱骗受体DcR1的表达、腰椎间盘中髓核组织细胞凋亡率。方法采用原位DNA片段末端标记法(TUNEL)和免疫组织化学方法对退变组的36例退变腰椎间盘髓核组织及对照组的22例创伤腰椎间盘髓核组织进行检测。结果36例退变腰椎间盘组织细胞凋亡率为52.41%,DR4细胞阳性率为53.24%,DcR1细胞阳性率为24.36%；22例创伤腰椎间盘组织细胞凋亡率为35.47%,DR4细胞阳性率为32.53%,DcR1细胞阳性率为30.54%。两组间细胞凋亡率、DR4蛋白表达差异均有统计学意义(P0.05)。两组髓核内DR4染色阳性细胞率与TUNEL阳性细胞率之间呈正相关（r=0.865,P0.05)。结论腰椎间盘中可能存在TRAIL/DR4

诱导的凋亡途径，DR4可能在腰椎间盘退行性变发生和发展中发挥重要作用。

【关键词】腰椎；椎间盘；凋亡；DR4；DcR1

The study on expression of apoptotic related DR4 and DcR1 in degenerative lumbar disc tissue HE Wen，XU Jie.Fujian Provincial Hospital,Provincial Clinical Medical College of Fujian Medical University,Fuzhou 350001,China

【Abstract】ObjectiveTo determine the apoptosis rate and the expressions of DR4 and DcR1 in human lumbar intervertebral discs.MethodsWe detected the apoptosis rate and the expression of DR4 and DcR1 in 36 cases of degenerative lumbar intervertebral disc tissues and 22 of traumatic normal lumbar intervertebral disc tissues with TdT-mediated dUTP biotinnick end labeling(TUNEL) and immunohistochemical staining methods.ResultsThe average apoptotic index(AI) and The expressions of DR4 protein in degenerative lumbar intervertebral discs was significantly higher than traumatic normal cases (52.41%vs 35.47%,P0.05).The DR4 positive incidence of nucleus pulposus showed significant positive correlation with the TUNEL positive incidence of nucleus pulposus

（r=0.865,P0.05).ConclusionDR4 may play an important role in the occurrence and progression of lumbar intervertebral disc degeneration, The apoptosis pathway induced by DR4/tumor necrotic factor-related apoptosis-inducing ligand(TRAIL)maybe exists in LID tissues.

【Key words】Lumbar vertebrae;Intervertebral disc;Apoptosis;DR4;DcR1

目前已发现3种诱导细胞凋亡的途径即FasL/Fas、TNF-α/TNFR1及TRAIL/TRAIL-R。相对于FasL/Fas、TNF-α/TNFR1介导的细胞凋亡途径在退变椎间盘中的研究，目前对于