Corning

Corning? CellBIND?表面培养皿促进脐带源间充质干细胞生长

宛莹1，贲亮1，官子楸2，李超1，张世冬1，聂德志1

1吉林省拓华生物科技有限公司，吉林省四平市136000；2康宁(上海)有限公司，上海市201206

Effects of Corning? CellBIND? Surface medium on growth of human umbilical cord mesenchymal stem cells

Wan Ying1, Ben Liang1, Guan Zi-qiu 2, Li Chao1, Zhang Shi-dong1, Nie De-zhi1

1 TuoHua Biological Technology Company, Siping 136000, Jilin Province, China

2 Corning Incorporated, Shanghai 201206, China

摘要

背景：培养体系微环境影响干细胞体外扩增，寻找有效的促进细胞贴壁和增殖的培养方法尤为重要。

目的：比较不同细胞培养材质对细胞体外扩增的影响。

方法：将5.0×104培养的人脐带源间充质干细胞分别接种于包被/不包被鸟氨酸Corning?聚苯乙烯培养皿或

Corning? CellBIND?表面培养皿中培养，分别观察细胞的贴壁、增殖状态、与细胞黏附和细胞增殖有关的蛋白的表达及细胞标志物的表达。

结果与结论：与其他类型的培养皿相比，包被多聚鸟氨酸的Corning? CellBIND?表面培养皿在24 h内能够促进人脐带源间充质干细胞贴壁，同时也能使培养的人脐带源间充质干细胞较早的进入对数生长期，细胞增殖数量相对较多，虽然对人脐带源间充质干细胞表面标志物CD73，CD90和CD105的表达没有影响，但能促进细胞中黏附蛋白的表达。且Corning? CellBIND?表面培养皿较Corning?聚苯乙烯培养皿更能促进人脐带源间充质干细胞的贴壁和增殖，同时也对细胞表型的表达无影响。提示Corning? CellBIND?表面培养皿能够促进细胞吸附，增加细胞数量，且对细胞周期蛋白以及细胞表型的表达没有影响，且包被多聚鸟氨酸能够进一步促进细胞的贴壁和增殖，并稳定表达人脐带源间充质干细胞的细胞表型。

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

关键词：干细胞；脐带脐血干细胞；干细胞基础实验；Corning?；CellBIND?表面培养皿；细胞贴壁；细胞增殖；细胞表型；脐带源间充质干细胞；多聚鸟氨酸；

Abstract：

BACKGROUND: Stem cells expansion technology in vitro is affected by the microenvironment of the culture system. So, to find an effective method is particularly important to promote cell adherent and growth. OBJECTIVE: To compare the effects of different culture media on cell expansion.

METHODS: Human umbilical cord mesenchymal stem cells at a density of 5.0×104 cells/cm2 were inoculated into Corning? polystyrene culture dishes coated with or without poly-L-ornithine and Corning? CellBIND culture dishes. Cell adhesion and proliferation were observed, and expressions of cell adhesion proteins and cell markers were detected.

RESULTS AND CONCLUSION: Cell adhesion was promoted when cells were cultured in Corning? CellBIND? Surface medium coated with poly-L-ornithine for 24 hours, and the cultured cells grew at the logarithmic phase. The cell proliferation was also enhanced, and the cells expressed cell adhesion protein but not the cell markers of CD73, CD90, CD105. Corning? CellBIND? Surface medium was superior to Corning? polystyrene culture medium in the improvement of cell adhesion and proliferation. Additionally, both of these two media showed no influence on cell phenotype. These findings indicate that Corning? CellBIND? Surface medium can

Dow Corning Rohs list

Product reference Contains substances restricted in RoHS and WEEE Directives * 1 CATALYST NO 1-2577 CONFORMAL COATING NO 1-2577 LOW VOC CONFORMAL COATING NO 1-2620 LOW VOC CONFORMAL COATING NO 1-4173 TC ADHESIVE NO 1000 PASTE NO 1000 SPRAY NO July 28th 2004 The following Dow Corning products have been reviewed for compliance with European Commission Directive 2002/95/EC relating to the Restriction of the use of certain Hazardous Substances in electrical & electronic equipment (RoHS Directive) and European Commission Directive 2002/96/EC relating to Waste Electrical & Electronic Equipment (WEEE Directive).

106 ANTI-FRICTION COATING NO 1102 GAS COCK GREASE NO 111 VALVE LUBE & SEALANT NO 1122 SPRAY NO 1200 OS PRIMER CLEAR NO 1200 PRIME COAT-RED NO 1200 PRIMER-CLEAR NO 1201 RTV PRIME COAT NO 1204 RTV PRIME COAT NO 1292 LONG LIFE BEARING GREASE NO 145 RTV ENCAPSULANT KIT NO 160 SILICONE ELASTOMER - PART A NO 160 SILICONE ELASTOMER - PART B NO 165 LT NO 170 FAST CURE KIT NO 170 SILICONE ELASTOMER KIT NO

Corning

Q: What is the recommended culture medium working volume in a Corning 5L Erlenmeyer flask?A: We recommend a working volume range of 2.5 to 3.0L of culture medium with Corning 5L Erlenmeyer flasks. Specifically for the baffled option, we recommend a working volume no less than 2.5L, the minimal volume for which the baffles were designed and optimized. Due to the height of the baffles, working volumes less than 2.5L could experience forces that take away the benefits of the baffles. Q: What is the recommended agitation rate for Corning 5L Erlenmeyer flasks? A: The optimal agitation rate will depend on the diameter of the orbit of the individual shaking platform, the cell type being cultured, the medium used and whether a baffled or plain bottom Erlenmeyer flask is used. With 25 mm orbit shakers, we recommend a rate of 90 rpm for CHO and Sf9 cells when cultured in Corning 5L Erlenmeyer flasks. It is important to empirically determine the agitation rate depending on conditions. This could be done by observing the amount of culture medium foaming with different agitation rates (90 to 120 rpm). The agitation rate with no or minimum foaming should be used as a good start-ing point. Typically we find the ideal rpm will need to be decreased as the size of the Erlenmeyer flask increases. Q: What is the recommended seeding density for 5L Erlenmeyer flasks? A: We recommend using the seeding density optimized for your given cell type or clone. Seeding den-sities of 0.3 to 0.5 x 106 cells/mL are typical for CHO cell culture and 0.5 x 106 to 1 x 106 cells/mL for Sf9 cell culture. Q: What is the recommended culture medium-feeding protocol in Corning 5L Erlenmeyer flasks?A: We recommend using the culture medium and feeding protocol (fed batch) optimized for your given cell type as a starting point. Some fine tuning of the feeding protocol may be needed for the maximum performance in 5L Erlenmeyer flasks. Q: What is the recommended scale up protocol for CHO cells in Corning 5L Erlenmeyer flasks?A: If starting from cryopreserved CHO cells, we recommend thawing cells into a Corning 125 mL Erlenmeyer flask using your standard protocol. Culture cells for several passages until cells are fully recovered and reach >2 x 106 cells/mL density by day 3 or 4. Always use cells from the mid logarithmic growth phase for the scale up. From 125 mL flask (40 mL working volume), scale up cells to 1L Corning Erlenmeyer flask (300 mL working volume), then to 5L Erlemeyer flask (2.5 to 3.0L working volume). It could take about 6 to 8 days to scale up from 125 mL to 5L Erlenmeyer flask depending on the cell growth rate. Q: Why might I want to use the baffled Corning 5L Erlnemeyer flask over a standard 5L Erlenmeyer flask?A: Usually we find that the baffles provide better aeration for cells, especially at a lower rpm. You may find that by using a baffled Erlenmeyer flask you can decrease the rpm from what you typically use.Q: Why is there an angled feature around the neck of the vessel? A: The angled feature located at the neck of the vessel will reduce foam when filling and pouring out of the vessel. Corning ? 5L Erlenmeyer Flask Frequently Asked Questions

Dowcorning 20 release coating

Product Information Release Dow Corning ?20Release Coating DESCRIPTION Dow Corning ? 20 Release Coating provides a durable parting film which effectively releases plastics, adhesives,and other elastomeric products that are sometimes difficult to release from mold surfaces, even with other silicones. HOW TO USE Dilution For easier application and finer control of the amount applied to mold surfaces, Dow Corning 20 Release Coating should be diluted to one part coating to 5-10 parts solvent . If wetting is a problem, blend 10 parts Release coating for plastics,adhesives,and elastomeric products APPLICATIONS ?In fabricating plastic parts, provides durable parting film for epoxies,melamimes, methacrylates, phenolics, polyesters, and polyurethanes.?Releases silicone glass laminates and tubes and parts made from silicone molding compounds. ?Provides release for wood forming operations, metal making, and rubber gaskets. TYPICAL PROPERTIES Specification Writers: These values are not intended for use in preparing specifications.Please contact your local Dow Corning sales office or your Global Dow Corning Connection before writing specifications on this product. CTM*Property Unit Value 0005 Color Colorless/light straw 0208Active material content % w/w 500001A Specific gravity @ 77°F (25°C)g/ml 0.930004Viscosity cst 11.00021A Flash point - closed cup °C 32 Solvent Stoddard and Xylene Suitable solvents Aliphatic and aromatic hydrocarbons CTM: Corporate Test Method, copies of CTMs are available on request. FEATURES ?Easy to use ?Does not discolor molded parts ?Solvent dilutable ? In most cases, a single application provides release through numerous molding cycles BENEFITS ?Provides heat stable, non-carbonizing, non-oily parting film ?Minimum build-up on molds and dies COMPOSITION ?Polydimethylsiloxane and high molecular weight silicone resin in solvent Dow Corning 20 Release Coating with 80 parts isopropanol (99%) and 10 parts toluene, rather than the previously mentioned solvents.Whenever using solvents, always provide adequate ventilation and follow the precautions on the manufacturer’s label. To break in molds with porous surfaces, a more concentrated solution – equal parts solvent and Dow Corning 20 Release Coating –may be used. Once the mold has been broken in, a more dilute solution may be used.

DOW CORNING 730

DOW CORNING 730 耐溶剂密封剂说明 DOW CORNING 730 耐溶剂密封剂是即取即用、不含溶剂、无坍塌的氟硅橡胶油膏，在潮湿的大气中形成坚韧的硅酮橡胶。它是一种抗化学侵蚀的液体密封剂，它适合于需抗燃料、油类及溶剂、烟灰、喷雾的场合，有时甚至需要整个浸入燃料、油类及溶剂的场合。优点 DOW CORNING 730 耐溶剂密封剂具有优良的抗气候、震动、潮湿、臭氧及极温性能。其在-57-+260℃的宽广温度范围都具有弹性，可以在零度以下的气候也不会丧失拉伸力及固化性能。 DOW CORNING 730 耐溶剂密封剂吸附于众多普通工业机械表面，能使用于过热或者垂直表面，不会松垂、下陷或脱离物体表面。应用 DOW CORNING 730 耐溶剂密封剂首要应用是作为在抗燃料、油类及溶剂有膨胀影响地方的结合、密封及敛缝用途。其已被证明在装配及修理燃料管道及车辆、油槽、小发动机、航空器及火箭上的应用是可接受的。其它典型应用包括：密封各式液体贮存槽接缝密封航天器及自动燃料管道及油槽暴露于腐蚀环境中橡胶衬套的暂时修补运输化学原料及溶剂管道接头的密封密封化学性压缩机，形成定位垫圈普通密封剂不能胜任的不规则形状及苛刻条件的地方形成定位垫圈长时间暴露于潮湿、震动、燃料、油类及溶剂组件的结合及密封应用于有甲酮分散的保护涂层绝缘体及在填注液体分配变压器入口密封，形成定位垫圈 DOW CORNING 730 耐溶剂密封剂类型单组份、通用氟硅酮橡胶粘合形态产品无坍塌油膏固化后坚韧、橡胶状固体固化暴露于潮湿空气下释放少量醋酸特性在一宽温度范围内可暴露于燃料、油类及溶剂中应用，具抗膨胀性能主要应用在需抗燃料、油类及溶剂的地方作粘合、密封及敛缝使用典型特性产品特性颜色………………………………………………….白色比重，25℃，………………………………………….1.40 挥发率，70℃，24小时，%…………………………………5 挤出率，1/8″管口，90psi,克/分……………………200 结皮时间，分…………………………………………..10 表干时间，分…………………………………………..25 流动性，MIL-S8802JIG ，厘米………………………0.254 25℃、50%相对湿度下固化7天后的特性肖式硬度，点…………………………………………..35 拉伸强度，Mpa ……………………………………….2.07 伸长率，%……………………………………………..175 撕裂强度，kN/m ………………………………………5.25 脆化点，℃…………………………………………-63℃ 具弹性下限，℃……………………………………-57℃ 有DOW CORNING 1200 底漆涂层铝合金Alcad2024T3 剥离强度，kN/m ………………………………………2.63 LAP 剪力，无底漆涂层铝合金Alcad2024T3 吸附力，kN/m ………………………………………45.53 有DOW CORNING 1200底漆涂层铝合金Alcad2024T3 吸附力，kN/m ………………………………………45.53 无底漆涂层冷卷钢吸附力，kN/m ………………………………………27.67 有DOW CORNING 1200底漆涂层的冷卷钢吸附力，kN/m ………………………………………42.03 使用 DOW CORNING 730 耐溶剂密封剂是即取即用的，在压力下，其自动从容器中挤出，膏状稠液体使其非常易于工作。 DOW CORNING 730 耐溶剂密封剂的固化过程是可以用油漆刀、搅拌器对表面进行加工，由表向里固化，在至少25℃

dowcorning 4

Product Information High Performance Lubricants FEATURES ?Maintain serviceable consistency from -70 to 400°F (-57 to 204°C)?Practically nonvolatile ?Odorless ?Resistant to a wide range of chemicals; generally resistant to mineral oils, vegetable oils, air, dilute acids, alkalines and most aqueous solutions; Dow Corning 111 Valve Lubricant & Sealant is quite resistant to a variety of organic and inorganic chemicals ?Moisture resistant ?Electrically insulating ?Excellent rubber lubrication ?Excellent release and sealing properties ?Resistant to oxidation ?Essentially nontoxic and non-melting ?Show little tendency to dry out in service COMPOSITION ?Greaselike materials containing an inert amorphous silica filler in combination with selected polydimethyl silicone fluids Dow Corning? 7 Release Compound, Dow Corning? 4 Electrical Insulating Compound and Dow Corning?111 Valve Lubricant & Sealant Dimethyl silicone compounds for a variety of lubrication and protection applications USES Dow Corning? 7 Release Compound can be used in applications including:?Mold release agent for foundry shell and core molds ?Break-in treatment for bladders on tire presses ?Rubber lubricant and preservative ?Release agent for adhesives and glues ?Airframe lubricant ?Cable-pulling lubricant to draw rubber-covered cable through conduit ?Release agent for plastic extruders and processing equipment ?Release agent for plastic film packaging machines Uses of Dow Corning? 4 Electrical Insulating Compound include:?Moisture-proof seal for aircraft, automotive and marine ignition systems and spark plug connections ?Waterproof electrical connections ?Electrical assemblies and terminals ?Seal and lubricant for cable connectors, battery terminals, rubber door seals and rubber and plastic O-rings ?Assembly lubricant for various metal-on-plastic and metal-on-rubber combinations ?Lubrication for control and pressure plug valves Dow Corning? 111 V alve Lubricant & Sealant can be used in applications such as:?Sealant for vacuum and pressure systems ?Sealant for outdoor equipment, including shipboard applications, subject to washing and harsh environments; meters; electrical service entrance and underground connections ?Damping medium for dash pots in electrical and electronic equipment ?Chemical barrier coating ?Antistick and sealant for transformer gaskets and equipment enclosures, which help prevent gaskets from sticking to metal and resist weathering and water washout ?Lubricant for rubber and plastic O-rings (see Use Limitations) ?Sealant for gaskets and seals ?Lubricant for water softener and faucet valves ?Valve stem lubricant for potable water ?Aircraft vacuum systems

dowcorning树脂和中间体

https://www.360docs.net/doc/d55021607.html,/coatings

Dow Corning
1
2
3
1
121°C (250°F) 121-204°C (250-400°F) 760°C (1400°F)
2 2
204-316°C (400-600°F)
3 3
316-427°C (600-800°F)
427-538°C (800-1000°F) 538-760°C (1000-1400°F)
1 2 3
100 15-50% 51-90%
50100% 2 6 VMS VOC VMS VMS
2

1.0/1.0 0.1-0.2%
https://www.360docs.net/doc/d55021607.html,/coatings 100% 232°C (450°F) 30 15-30 50-80% 218°C (425°F) coatings@https://www.360docs.net/doc/d55021607.html,
3

409 HS
?
431 HS
804
805
806A
808
840
52 1 60 1.1/1 2000-7000
52 64 3 3 66 68 1.2/1 0.4/1 2000-7000 2000-7000
52 1 60 1.1/1 200,000300,000
52 1 60 1.1/1 200,000300,000
57 1 63 0.7/1 200,000300,000
52 3 66 1.2/1 2000-7000
80 74
80 74
60 51
50 42
50 41
50 42
60 51
VOC , g/l (lb/gal) °C (°F)
1.12 228 (1.9) 200 27 (81)
1.14 228 (1.9) 800 7 (45)
1.07 431 (3.6) 30 7 (45)
1.01 503 (4.2) 125 27 (81)
1.02 515 (4.3) 150 7 (45)
1.01 503 (4.2) 125 27 (81)
1.06 431 (3.6) 20 7 (45)
217
?
220
233
249
Z-6018
3037
3074
QP85314
47 6 75 n/a 15002500
52 1 70 2.0/1 20004000
52 5 71 1.3/1 20004000
63 5 71 0.6/1 20004000
51 6 75 2.7/1 15002500
65 15-18 58 0.5/1 8001300
54 15-18 67 1.0/1 10001500
46 35 68 3.3/1
100 1.34 138 (280)
100 1.33 138 (280)
100 1.32 138 (280)
50%
100 1.30 138 (280)
100 1.31 138 (280)
90 1.07 14 138 (280)
90 1.16 120 138 (280)
100 1.04 1.87 28 (83)
VOC , g/l (lb/gal) °C (°F)
1 2 3 4 5 6 7
SiO2 1.5 g
100% 3 EPA 24
CH3 2SiO x
135°C 275°F
4

DOW CORNING 200(R) FLUID, 350 MSDS

Revision: Current revision, change description 版本：A , 变更理由 Initial Release EPM-NKG Proprietary Statement This document is confidential, contains proprietary information, and should not be distributed, copied or otherwise reproduced without the express written consent of EPM-NKG, Inc. 物料安全数据表产品名称 DOW CORNING 200(R) FLUID, 350 CST 企业识别略成分/组分信息 ? 危险组分：无。此产品不属于职业安全与卫生条例（OSHA ）危害通识标准列入的危险物质。危害识别 ? 潜在的健康影响急性影响： ? 眼睛接触：直接接触会导致短暂的发红和不适。 ? 皮肤接触：单一短时间暴露不会有重大影响。 ? 食入：正常使用情况下食入的危害较低。 ? 吸入：单一短时间暴露不会有重大影响。长期/重复接触的影响： ? 皮肤：无适用信息 ? 吸入：无适用信息 ? 食入：无适用信息 ? 过度接触的症状：无适用信息急救措施 ? 眼睛：立即用水冲洗。 ? 皮肤：无需急救。 ? 吸入：无需急救。 ? 食入：无需急救。 ? 建议：对症医治。消防措施 ? 闪点：>101.1℃（闭杯测试） ? 自燃温度：未测定 ? 爆炸极限：未测定 ? 灭火剂：大火时使用干化学品、泡沫、水喷射；小火时用二氧化碳、干化学品或者水喷射。水可以用来冷却大火燃烧暴露出的容器。 ? 消防程序：扑灭涉及化学品的大火时需佩戴自给式呼吸器及穿好防护衣，根据当地的应急计划决定是否需要撤离或者隔离该区域，用水喷射冷却大火燃烧暴露出的容器。

CorningCoolCell程序降温盒使用说明

Corning? CoolCell?程序降温盒使用说明快速使用 ●将核心金属环（黑环）放置在降温盒中央底部（若金属环刚刚使用过，再次使用前应确保首先平衡到室温） ●将装有1mL细胞悬液的冻存管放入管孔中。如果单次冻存量少于12管，用CoolCell 填充管（货号432076）或其他装有相同体积冷冻介质的冻存管填满空位 ●盖上CoolCell盖子，对齐并盖紧 ●将CoolCell直立放入-80°C冰箱或干冰柜中，CoolCell四周至少留有一英寸（2.54cm）空隙，确保散热均匀 ●冷冻四小时后（或更久），转移到液氮罐。注意事项：管孔和冻存管应保持干燥，避免冻存管与管孔冷冻之后粘连。 CoolCell温度最好与细胞悬液温度相同（例如，都平衡到室温）。转移储存冷冻样品 ●准备一个隔热托盘或其他容器，放入2.5cm高度的粉状/颗粒状干冰 ●从冰箱中取出CoolCell，通过轻柔转动或小幅度摇摆，打开盖子 ●将CoolCell倒转，把冻存管倒到干冰上，并用干冰覆盖。检查CoolCell中是否有未倒出的冻存管。如果有冻存管粘在CoolCell中，可将CoolCell反转放于平坦的桌面或手掌上，拍打底部，将冻存管倒出。注意事项当转移冻存管时，一定确保使用干冰来避免温度升高，细胞损伤。暴露于室温的冻存管。在不到一分钟的时间温度可从- 80°C上升到高于-50°C。强烈建议在储种培养结束之前对所有冻存复苏的培养细胞确定活率

循环使用当CoolCell 泡沫盒和金属环恢复到室温时，便可再次使用。为了使CoolCell 容器快速达到室温并循环使用，可将金属环从泡沫盒中取出。CoolCell 泡沫底托和泡沫盖可在10到15分钟内恢复到室温。再次使用前，确保所有的管孔和金属环干燥，防止粘连。关于CoolCell CoolCell 与-80°C 冰箱或干冰柜配合使用，将提供-1°C/min 的最佳冷冻速率，适合绝大多数细胞冻存操作。CoolCell 容器的设计结合使用了绝缘泡沫，对称辐射，和热传导核心均匀调节热量流失，而不是利用大的散热装置（异丙醇依赖型冻存盒）来降温。独特的产品设计确保每个冻存管的温度十分一致。CoolCell 散热小，不会造成冰箱内局部温度上升，影响冰箱中的其他样品；同时也意味着CoolCell 可以迅速恢复到室温，进行下一次的循环使用（见上述循环使用章节）。常见故障排除问题解决方法冻存管不能轻松地插入管孔内 CoolCell 容器设计满足标准的螺旋盖式1mL 和2mL 冻存管，直径不超过13mm ，高度不超过55mm 。如果在冻存管外壁贴有标签纸，确保标签纸不会造成妨碍。冷冻后，冻存管粘连在CoolCell 内很可能是管孔或冻存管表面有水分。去除金属环，倒置CoolCell ，拍打底部，倒出。盖子不能完全盖上确保样品管完全插入孔内。可容纳的冻存管最大高度为55mm 。护理和清洁 ● CoolCell 容器由封闭的交联聚乙烯泡沫和固体热传导核心组成，CoolCell 容器可长期暴露于超低温或低温环境。 ● 泡沫可由水和温和的肥皂清洗，彻底冲洗并完全干燥。 ● CoolCell 容器可耐受酒精和10%漂白粉溶液消毒。 ● 最高暴露温度为60℃，不可高压灭菌。 ● 避免长时间暴露于紫外线光源。 CoolCell 冷冻性能 CoolCell 容器可一次冻存12只冻存管（1mL 细胞悬液/管），当放置在-75°C 到-80°C 环境下（超低温冰箱或干冰柜），可以-1℃ /min 速度均匀降温。左侧五个连续的冷冻剖面曲线反应了12个样品负载下的情况。

Dow Corning 3165

)NFORMATION ABOUT $OW #ORNING ¤ BRAND !DHESIVE 3EALANTS 3ILICONES AND %LECTRONICS ,ONG TERM RELIABLE PROTECTION OF SENSITIVE CIRCUITS AND COMPONENTS IS IMPORTANT IN MANY OF TODAY S DELICATE AND DEMANDING ELECTRONIC APPLICATIONS 3ILICONES FUNCTION AS DURABLE DIELECTRIC INSULATION AS BARRIERS AGAINST ENVIRON MENTAL CONTAMINANTS AND AS STRESS RELIEVING SHOCK AND VIBRATION ABSORBERS OVER A WIDE TEMPERATURE AND HUMIDITY RANGE )N ADDITION TO SUSTAINING THEIR PHYSICAL AND ELECTRICAL PROPERTIES OVER A BROAD RANGE OF OPERATING CONDITIONS SILICONES ARE RESISTANT TO OZONE AND ULTRAVIOLET DEGRADATION HAVE GOOD CHEMICAL STABILITY AND ARE AVAILABLE IN A VARIETY OF USEFUL FORMS AS CONFORMAL COATINGS ENCAPSULANTS AND ADHESIVES $OW #ORNING S BROAD RANGE OF GENERAL PURPOSE AND SPECIALTY PRODUCTS OFFERS YOU A CHOICE OF MATERIALS FOR YOUR APPLICATION NEEDS $OW #ORNING OFFERS A VARIETY OF NONCORROSIVE SILICONE PRODUCTS FOR ELECTRONIC SEALING BONDING AND ADHERING APPLICATIONS 4HESE ADHESIVES GENERALLY FALL INTO THREE CURE TYPES 4HE lRST GROUP ARE MOISTURE CURE GENERALLY MEANT FOR ROOM TEMPERATURE PROCESSING 4HE SECOND TYPE CONDEN SATION CURE OFFERS RAPID ROOM TEMPERATURE AND DEEP SECTION CURING 4HE THIRD TYPE IS HEAT CURE FOR RAPID PROCESSING !LL CONVERT TO DURABLE RELATIVELY LOW STRESS ELASTOMERS -OST WILL DEVELOP GOOD PRIMERLESS ADHESION TO A VARIETY OF COMMON SUBSTRATES INCLUDING CERAMICS REACTIVE METALS AND lLLED PLASTICS #52% #/.$)4)/.3 /NE 0ART -OISTURE #URE 246 4HE ONE PART MOISTURE CURE ADHESIVES AND ARE GENERALLY CURED AT ROOM TEMPERATURE AND IN A RANGE OF TO PERCENT RELATIVE HUMIDITY 'REATER THAN PERCENT OF THEIR FULL PHYSICAL PROPERTIES SHOULD BE ATTAINED WITHIN TO HOURS DEPENDING ON THE PRODUCT CHOSEN -ATERIALS AND PARTS CAN BE HANDLED IN MUCH SHORTER TIMES OF ABOUT TO MINUTES DEPENDING ON THE PRODUCT CHOSEN AND THE AMOUNT OF MATERIAL USED PER PART 4HESE MATERIALS ARE NOT TYPICALLY USED FOR HIGHLY CONlNED OR DEEP SECTION CURES -ATERIALS WILL GENERALLY CURE ABOUT INCH PER SEVEN DAYS FROM ANY EXPOSED SURFACE #URE PROGRESSES FROM THE OUTER SURFACE AND IS DEPENDENT ON THE MOISTURE IN THE AIR 7ORKING TIME IS GENERALLY A FEW MINUTES TO AN

Dow corning 3145 RTV

Page: 1 of 8 Version: 3.1 Revision Date: 2008/06/18 DOW CORNING(R) 3145 RTV MIL-A-46146 ADHESIVE/SEALANT-CLEAR 1. IDENTIFICATION OF THE SUBSTANCE AND OF THE COMPANY Dow Corning Corporation South Saginaw Road Midland, Michigan 48686 24 Hour Emergency Telephone: Customer Service: Product Disposal Information: CHEMTREC: (989) 496-5900 (989) 496-6000 (989) 496-6315 (800) 424-9300 MSDS No.: 01190300 Revision Date: 2008/06/18 Generic Description: Silicone elastomer Physical Form: Paste Color: Translucent white Odor: Slight odor NFPA Profile: Health1Flammability1Instability/Reactivity0 Note: NFPA = National Fire Protection Association 2. HAZARDS IDENTIFICATION POTENTIAL HEALTH EFFECTS Acute Effects Eye: Direct contact may cause mild irritation. Skin: No significant irritation expected from a single short-term exposure. Inhalation: Vapor may irritate nose and throat. Vapor overexposure may cause drowsiness. Oral: Overexposure by ingestion may cause effects similar to those listed under repeated exposure. Prolonged/Repeated Exposure Effects Skin: Repeated or prolonged exposure may cause irritation. Inhalation: Product generates methyl alcohol which may cause blindness and damage to nervous system. Oral: Product generates methyl alcohol which may cause blindness and possibly death if swallowed. Signs and Symptoms of Overexposure No known applicable information. Medical Conditions Aggravated by Exposure