遗传性嗜铬细胞瘤家系血浆儿茶酚胺及其代谢物的含量测定

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作者：刘洪旭邓思珊马丽红马振菁王新康

来源：《中国医药导报》2019年第06期

[摘要] 目的建立高效液相色谱-电化学检测器（HPLC-ECD）法测定遗传性嗜铬细胞瘤家系血浆儿茶酚胺及其代谢产物的含量的方法。方法工作电极为玻璃电极，参比电极为Ag-AgCl电极，电压0.6 V，温度30℃。色谱柱：Ultimate XB-C18（4.6 mm×150 mm，5 μm）;以甲醇为流动相A，0.1%磷酸二氢钠（含EDTA-Na和辛烷磺酸）水溶液为流动相B，梯度洗脱，流速为0.8 mL/min;柱温为室温;检测波长：280 nm;进样量：20 μL。结果正常健康人中肾上腺素（E）、去甲肾上腺素（NE）、甲氧基去甲肾上腺素（NMN）、甲氧基肾上腺素（MN）分别为（56.71±25.67）、（98.62±36.90）、（78.36±22.36）、（33.27±14.62） ng/L。嗜铬细胞瘤家系中确诊的2例嗜铬细胞瘤患者的NE分别为353.36、563.87 ng/L和NMN分别为425.71、527.65 ng/L，其余18位家属的相关检测值在正常健康成人的范围内，其他2位家属的相关检测值比正常值高，建议去医院进一步检查。结论该方法简便，灵敏、准确，可用于临床嗜铬细胞瘤患者的检测。

[关键词] 高效液相色谱-电化学检测器;嗜铬细胞瘤;儿茶酚胺;代谢

[中图分类号] R586.2; ; ; ; ; [文献标识码] A; ; ; ; ; [文章编号] 1673-7210（2019）02（c）-0113-04

[Abstract] Objective To establish a method for the determination of catecholamines and their metabolites in plasma of hereditary pheochromocytoma families by high performance liquid chromatography-electrochemical detector （HPLC-ECD）. Methods The working electrode was a glass electrode and the reference electrode was an Ag-AgCl electrode with a voltage of 0.6 V and a temperature of 30℃. Using Ultimate XB-C18 （4.6 mm × 150 mm，5 μm）; using methanol as mobile phase A， 0.1% sodium dihydrogen phosphate （containing EDTA-Na and octanesulfonic acid） aqueous solution as mobile phase B， gradient elution， flow rate 0.8 mL/min; column temperature was room temperature; detection wavelength was 280 nm; injection volume was 20 μL. Results In normal healthy people， the contents of epinephrine（E）， norepinephrine（NE），methoxy norepinephrine （NMN）， methoxy-adrenalin （MN） were （56.71±25.67），

（98.62±36.90），（78.36±22.36），（33.27±14.62） ng/L. The NEs of 2 patients with pheochromocytoma diagnosed in pheochromocytoma were 353.36， 563.87 ng/L and NMN were 425.71 and 527.65 ng/L， respectively. The correlation detection values of the other 18 family members were within the range of normal healthy adults. The correlation detection values of the other two family members were higher than the normal values. it is recommended to go to the hospital for further examination. Conclusion This method is simple， sensitive and accurate and can be used for the detection of patients with clinical pheochromocytoma.