常用引物

引物名称 5’-3’备注ITS2 156AACCATCGAGTCTTTGAACGC

ITS2 157CCTTGTAAGTTTCTTTTCCTCC COI f1-1ACGGTTCAGCCTGTTCCTGC

COI r1-1GTATGAGCCCACCACATATT COI LCO1490GGTCAACAAATCATAAAGATATTGG COI HCO2198TAAACTTCAGGGTGACCAAAAAATCA ITS 1F CCGCAGGTTCACCTACGGA

ITS 4R GCATATCAATAAGCGGAGGA

ITS2 2F ATGCGATACTTGGTGTGAAT

ITS2 3R GACGCTTCTCCAGACTACAAT

ITS 4R TCCTCCGCTTATTGATATGC

ITS 5F GGAAGTAAAAGTCGTAACAAGG

ITS JF CGACAAGTATCCTTCCGCAG

ITS 4R TCCTCCGCTTATTGATATGC

matK-1326R

TCTAGCACACGAAAGTCGAAGT matK-390F

CGATCTATTCATTCAATATTTC matK Kim_3F

CGTACAGTACTTTTGTGTTTACGAG matK Kim_1R

ACCCAGTCCATCTGGAAATCTTGGTTC rbcL 1F

ATGTCACCACAAACAGAAAC rbcL 724R

TCGCATGTACCTGCAGTAGC

PA GTTATGCATGAACGTAATGCTC

TH CGCGCATGGTGGATTCACAATCC rnL-F-c CGAAATCGGTAGACGCTACG

trnL-F-f ATTTGAACTGGTGACACGAG

ITS2 P3YGACTCTCGGCAACGGATA

ITS2 E4RGTTTCTTTCCTCCGCTTA

trnL g GGGCAATCCTGAGCCAA

trnL h CCATTGAGTCTCTGCACCTATC 扩灵芝的ITS2引物扩蕨类的ITS引物

扩小片段