Gene7-12

12.4 Each eukaryotic chromosome contains many replicons
S phase is the restricted part of the eukaryotic cell cycle during which synthesis of DNA occurs.
Chapter 12
The replicon
12.1 Introduction 12.2 Replicons can be linear or circular 12.3 Origins can be mapped by autoradiography and electrophoresis 12.4 The bacterial genome is a single circular replicon 12.5 Each eukaryotic chromosome contains many replicons 12.6 Isolating the origins of yeast replicons 12.7 D loops maintain mitochondrial origins 12.8 The problem of linear replicons 12.9 Rolling circles produce multimers of a replicon 12.10 Rolling circles are used to replicate phage genomes 12.11 The F plasmid is transferred by conjugation between bacteria 12.12 Conjugation transfers single-stranded DNA 12.13 Connecting bacterial replication to the cell cycle 12.14 Cell division and chromosome segregation 12.15 The division apparatus consists of cytoskeletal and regulatory components 12.16 Partitioning involves membrane attachment and (possibly) a motor 12.17 Multiple systems ensure plasmid survival in bacterial populations 12.18 Plasmid incompatibility is determined by the replicon 12.19 The ColE1 compatibility system is controlled by an RNA regulator
12.4 Each eukaryotic chromosome contains many replicons Figure 12.8 Measuring the size of the replicon requires a stretch of DNA in which adjacent replicons are active.
12.6 D loops maintain mitochondrial origins
D loop is a region within mitochondrial DNA in which a short stretch of RNA is paired with one strand of DNA, displacing the original partner DNA strand in this region. The same term is used also to describe the displacement of a region of one strand of duplex DNA by a single-stranded invader in the reaction catalyzed by RecA protein.
12.4 Each eukaryotic chromosome contains many replicons
Figure 12.5 Different densities of radioactive labeling can be used to distinguish unidirectional and bidirectional replication.
12.2 Origins can be mapped by autoradiography and electrophoresis
Figure 12.3 A replication eye forms a theta structure in circular DNA.
12.2 Origins can be mapped by autoradiography and electrophoresis Figure 12.4 The replication eye becomes larger as the replication forks proceed along the replicon. Note that the "eye" becomes larger than the nonreplicated segment. The two sides of the eye can be defined because they are both the same length. Photograph kindly provided by Bernard Hirt.
12.4 Each eukaryotic chromosome contains many replicons
Figure 12.9 Replication forks are organized into foci in the nucleus. Cells were labeled with BrdU. The leftmost panel was stained with propidium iodide to identify bulk DNA. The right panel was stained using an antibody to BrdU to identify replicating DNA.
12.2 Origins Fra bibliotekan be mapped by autoradiography and electrophoresis
Figure 12.6 The position of the origin and the number of replicating forks determine the shape of a replicating restriction fragment, which can be followed by its electrophoretic path (solid line). The dashed line shows the path for a linear DNA.
12.1 Introduction
Replicon is a unit of the genome in which DNA is replicated; contains an origin for initiation of replication.
12.1 Introduction
Replicon is a unit of the genome in which DNA is replicated; contains an origin for initiation of replication.
12.1 Introduction
Figure 11.2 Several types of independent genetic units exist in bacteria.
12.2 Origins can be mapped by autoradiography and electrophoresis
12.3 The bacterial genome is a single circular replicon
Figure 12.7 Replication termini in E. coli are located beyond the point at which the replication forks actually meet.
Replication fork is the point at which strands of parental duplex DNA are separated so that replication can proceed.
12.2 Origins can be mapped by autoradiography and electrophoresis
12.2 Origins can be mapped by autoradiography and electrophoresis
Figure 12.5 Different densities of radioactive labeling can be used to distinguish unidirectional and bidirectional replication.
12.3 The bacterial genome is a single circular replicon
Figure 12.7 Replication termini in E. coli are located beyond the point at which the replication forks actually meet.
Figure 12.1 Replicated DNA is seen as a replication eye flanked by nonreplicated DNA.