定语从句精选50练习题及答案

定语从句精选50练习题及答案
定语从句精选50练习题及答案

定语从句练习题

1.The place _______interested me most was the Children's Palace.

A. Which

B. where

C. what

D. in which

2.Do you know the man _______?

A. whom I spoke

B. to who spoke

C. I spoke to

D. that I spoke

3.This is the hotel _______last month.

A. which they stayed

B. at that they stayed

C. where they stayed at

D. where they stayed

4.Do you know the year ______the Chinese Communist Party was founded?

A. which

B. that

C. when

D. on which

5.That is the day ______I'll never forget.

A. which

B. on which

C. in which

D. when

6.The factory ______we'll visit next week is not far from here.

A. where

B. to which

C. which

D. in which

7.Great changes have taken place since then in the factory _______we are working.

A. where

B. that

C. which

D. there

8.This is one of the best films _______.

A. that have been shown this year

B. that have shown

C. that has been shown this year

D. that you talked

9.Can you lend me the book ______the other day?

A. about which you talked

B. which you talked

C. about that you talked

D. that you talked

10.The pen ______he is writing is mine.

A. with which

B. in which

C. on which

D. by which

11.They arrived at a farmhouse, in front of ______sat a small boy.

A. whom

B. who

C. which

D. that

12.The engineer ______my father works is about 50 years old.

A. to whom

B. on whom

C. with which

D. with whom

13.It there anyone in your class ______family is in the country?

A. who

B. who's

C. which

D. whose

14.I'm interested in ______you have said.

A. all that

B. all what

C. that

D. which

15.I want to use the same dictionary ______was used yesterday.

A. which

B. who

C. what

D. as

16.He isn't such a man ______he used to be.

A. who

B. whom

C. that

D. as

17.He is good at English, ______we all know.

A. that

B. as

C. whom

D. what

18.Li Ming, ______to the concert enjoyed it very much.

A. I went with

B. with whom I went

C. with who I went

D.I went with him

19.I don't like ______ as you read.

A. the novels

B. the such novels

C. such novels

D. same novels

20.He talked a lot about things and persons ________they remembered in the school.

A. which

B. that

C. whom

D. what

21.The letter is from my sister, ______is working in Beijing.

A. which

B. that

C. whom

D. who

22.In our factory there are 2,000 workers, two thirds of ____are women.

A. them

B. which

C. whom

D. who

23.You're the only person ______I've ever met ______could do it.

A. who;/

B./; whom

C. whom;/

D./; who

24.I lost a book, ______I can't remember now.

A. whose title

B. its title

C. the title of it

D. the title of that

https://www.360docs.net/doc/5412215663.html,st summer we visited the West Lake, ______Hangzhou is famous in the world.

A. for which

B. for that

C. in which

D. what

26.I have bought such a watch _______ was advertised on TV.

A. that

B. which

C. as

D. it

27.I can never forget the day _______ we worked together and the day ______ we spent together.

A. when; which

B. which; when

C. what; that

D. on which; when

28.The way ______he looks at problems is wrong.

A. which

B. whose

C. what

D./

29.This is the reason ______he didn't come to the meeting.

A. in which

B. with which

C. that

D. for which

30.This machine, ______for many years, is still working perfectly.

A. after which I have looked

B. which I have looked after

C. that I have looked after

D. I have looked after

31.The reason ______he didn't come was ______he was ill.

A. why; that

B.that;why

C. for that;that

D.for which;what

32.He is working hard, ______will make him pass the final exam.

A.that

B.which

C.for which

D.who

33.That is not the way ______I do it.

A./

B.which

C.for which

D.with which

34.I have two grammars, ______are of great use.

A. all of which

B. either of which

C. both of that

D. both of which

35.I want to use the same tools _______used in your factory a few days ago.

A. as was

B. which was

C. as were

D. which

36.My neigh bours used to give me a hand in time of trouble, _______ was very kind of them.

A. who

B. which

C. that

D. it

37.This is the magazine _______ I copied the paragraph.

A. that

B. which

C. from that

D. from which

38.He is not such a man _______ would leave his work half done.

A. that

B. which

C. who

D. as

39. You can depend on whatever promise _______ he makes.

A. /

B. why

C. when

D. whose

40. Smoking, _______ is a bad habit, is, however, popular.

A. that

B. which

C. it

D. though

41. --- Did you ask the guard _______ happened?

--- Yes, he told me all _______ he knew.

A. what; that

B. what; what

C. which; which

D. that; that

42. I shall never forget those years _______ I lived on the farm with

the farmers, _______ has a great effect on my life.

A. when; who

B. that; which

C. which; that

D. when; which

43. The number of the people who _______ cars _______ increasing.

A. owns; are

B. owns; is

C. own; is

D. own; are

44. During the days ________, he worked as a servant at the Browns.

A. followed

B. following

C. to follow

D. that followed

45. Is oxygen the only gas _______ helps fire burn?

A. that

B. /

C. which

D. it

46. The clever boy made a hole in the wall, _______ he could see _____ was going on inside house.

A. which; what

B. through which; what

C. through that; what

D. what; that

47. Is _______ some German friends visited last week?

A. this school

B. this the school

C. this school one

D. this school where

48. John got beaten in the game, _______ had been expected.

A. as

B. that

C. what

D. who

49. I have bought two ballpens, _______ writes well.

A. none of them

B. neither of them

C. neither of which

D. none of which

50. All that can be eaten _______ eaten up.

A. are being

B. has been

C. had been

D. have been

高中非谓语动词练习A

1. When he was a boy, he used to go there and watch _____.

A. to repair bicycles

B. bicycles to be repaired

C. bicycles being repaired

D. repairing bicycles

2. --- Have you considered _____ your job as a teacher?

--- Yes. I like the job because a teacher is often considered _____ a gardener.

A. to change; to be

B. to change; being

C. changing; being

D. changing; to be

3. The dog, _____, will be made a good watchdog.

A. to train properly

B. being trained properly

C. properly to train

D. trained properly

4. _____ the diamond, he had to look for a place to hide it.

A. Having stolen

B. Having been stolen

C. Stolen

D. Stealing

5. _____ the front door _____, he had to enter the room through the back door.

A. Seen; painted

B. Seeing; painted

C. Being seen; being painted

D. Seeing; being painted

6. And there, almost _____ in the big chair, sat her little brother, who never had to be told to keep quiet.

A. having lost

B. losing

C. to be lost

D. lost

7. He looked around and caught a man _____ his hand into the pocket of a passenger.

A. put

B. to be putting

C. to put

D. putting

8. To answer correctly is more important than _____.

A. that you finish quickly

B. finishing quickly

C. to finish quickly

D. finish quickly

9. The old farmer, _____ the badly injured and burnt soldier, came out of the burning farmhouse, calling continuously for help.

A. supporting

B. having supported

C. being supported by

D. being supported

10. In January , 2004, the United States successfully launched “Spirit”, a Mars Exploration Rover, _____ a new milestone in the history of mankind.

A. it marked

B. marking

C. marked

D. to mark

11. It was getting dark; I found a car _____ in a pool by the side of the road.

A. to be stuck

B. stuck

C. sticking

D. stick

12. You can’t imagine what great trouble they have _____ the problem _____.

A. to solve; being talked about

B. solving; discussing

C. to solve; to talk about

D. solving; being discussed

13. The monument was built in honor of the explorer who was believed _____ the river.

A. to have discovered

B. to have been discovered

C. to discover

D. having been discovered

14. The thief fell to the ground, his left foot _____ and blood _____ down from his mouth.

A. breaking; running

B. broken; running

C. breaking; run

D. broken; run

15. The program was so exciting that the children kept their eyes _____ on the screen.

A. to fix

B. to be fixed

C. fixed

D. fixing

16. As I will be away for at least a year, I’d appreciate _____ from you now and then so that I can know how everyone is gett ing along.

A. having heard

B. to hear

C. hearing

D. being heard

17. _____, the subject was always in my mind.

A. Walking or sleeping

B. Walking or slept

C. Having walked or slept

D. To walk and sleep

18. --- Did you get a dictionary?

--- No, I _____, but there were not any _____.

A. tried; to be left

B. had tried to; leaving

C. tried to; left

D. had tried; have left

19. When he came to, he found himself _____ on a chair, with his hands _____ back.

A. to sit; tying

B. sitting; tying

C. seating; tied

D. seated; tied

20. The joke told by Tom made us _____, so the teacher couldn’t make himself _____.

A. to laugh; hearing

B. laughing; heard

C. laughing; hear

D. laughed; heard

21. All of the flowers now _____ here have developed from those once _____ in the forest.

A. raised; grown

B. rising; growing

C. raised; growing

D. rising; grown

22. Henry can’t attend the party _____ at Tom’s house at present because he is preparing the speech at the party _____ at Marie’s house tomorrow.

A. held; being held

B. to be held; to be held

C. to be held; held

D. being held; to be held

23. Seeing the soldiers well _____ for the flood-fight, the general nodded with satisfaction.

A. prepare

B. preparing

C. prepared

D. having prepared

24. Don’t worry. I’ll have it _____ and get someone _____ it to you tomorrow.

A. typed; send

B. to type; to send

C. type; send

D. typed; to send

25. The sun was shining brightly, _____ everything there _____ more beautiful.

A. making; look

B. to make; looked

C. and made; looking

D. and making; be looked

26. How could he get the plan _____ without any one to support him?

A. to carry out

B. carry out

C. carrying out

D. carried out

27. New ideas sometimes have to wait for years before _____.

A. being fully accepting

B. fully accepting

C. having fully accepted

D. fully accepted

28. Having passed all the tests, she felt a great weight _____ off her mind.

A. taking

B. taken

C. take

D. to be taken

29. I’m examining the article he has just finished _____ the possible mista kes in it.

A. being corrected

B. to correct

C. corrected

D. having corrected

30. _____ full preparations, we decided to put off the meeting till next week.

A. We did not make

B. Having not made

C. We had not made

D. Not having made

31. We should prevent such a silly mistake _____ again.

A. occurring

B. to occur

C. to be occurred

D. from being occurred

32. I regret _____ hard at school, or I would have succeeded in passing the exam.

A. not to work

B. having not worked

C. to have not worked

D. not having worked

33. They looked forward with hope _____ a chance to receive further education.

A. for getting

B. of getting

C. to get

D. to getting

34. “Well, I’ll tell you a secret ...” said the boy, with his mouth almost _____ my ear.

A. touched

B. touching

C. touch

D. to touch

35. Towards evening, the patient opened his mouth as if _____ something to his son.

A. said

B. say

C. to say

D. to have said

36. --- Would you be _____ lend me your bicycle?

--- Sure.

A. so kind as to

B. kind enough as to

C. very kind to

D. so kind to

37. The beach is so beautiful that it is worthwhile, I think, _____ for a short holiday.

A. of going

B. to be going

C. your going

D. you to go

38. The teacher asked more _____ to prevent the students’ eyes from being injured.

A. to do

B. done

C. to be done

D. being done

39. Some of the schools in Shanghai have moved one step closer to _____ with the global education community.

A. being connected

B. connect

C. having connected

D. be connected

40. The performance of the host, _____ to please the audience and draw their attention, was greeted with a cold silence, however.

A. had intended

B. intended

C. being intended

D. to intend

41. _____ and out of breath, we reached the top of the mountain and stopped _____ the beautiful scenery.

A. Tiring; to admire

B. Being tired; admiring

C. Tired; to admire

D. Tired; admiring

42. The 18-storeyed building, when _____, will shut out the sun _____ up the rooms in my house.

A. completed; lighted

B. completing; lighting

C. completing; lighted

D. completed; lighting

43. --- I hear Warren _____ in a middle school.

--- What? I can’t imagine him _____ as a teacher.

A. teaches; working

B. teaches; work

C. teach; to work

D. teach; working

44. _____ he’s only been learning English for a year, he speaks it very well.

A. Considered

B. Considering

C. Having considered

D. To consider

45. It was _____ computer games that cost the boy a lot of time that he ought to have spent on his lessons.

A. to have played

B. playing

C. played

D. having played

46. At no time does the salesgirl get up late in the morning, for she is always too busy _____ a good rest.

A. to take

B. taking

C. took

D. taken

47. _____ as the most excellent student in her university, as most classmates had expected, made her parents very happy.

A. Mary was chosen

B. Mary chosen

C. Mary being chosen

D. Mary’s being chosen

48. _____ the big snake, the little girl stood under the tree _____ to death.

A. Seeing; frightened

B. Seeing; frightening

C. Seen; frighten

D. To see; frightening

49. You will see this product made in this factory _____ wherever you go.

A. to be advertised

B. advertised

C. advertise

D. advertising

50. Mr. Green is said _____ an experiment to prove the new method of solving the problem when young.

A. to do

B. to have done

C. to be doing

D. to have been doing

高中非谓语动词练习B

1__Sunday, the students are at home. A. Being B. To be C. It is D. It being

2.The boy lay on his back, his teeth __and his glaring eyes ___.

A.set;looked

B. set;looking

C. setting;looked

D. setting;looking

3.All the thing____,his proposal is of greater value than yours.

A. considered

B. considering

C. to consider

D. consider

4.____who she was, she said she was Mr.Johnson’s friend.

A. Asking

B. Asked

C. To be asked

D. When asking

5.He hurried to the station,___the 9:30 train had already left.

A. to find

B. found

C. only to find

D. only finding

6.Which do you enjoy ___your weekends, fishing or watching TV?

A.spending

B.to spend

C.being spent

D.spend

7.___all my letters, I had a drink and went out. A.Finished B.Having finished C.Finishing D.To finish

8.She had no money ___a birthday present for her children.

A. to buy with

B. buying C .bought D. with which to buy

9.From the dates___on the gold coin, it is conformed that it was made five hundred years ago.

A. marking

B. marked

C. to be marked

D. having been marked

10.____time and labour, cartoonists generally draw the hands of their characters with only 3 fingers

and a thumb. A. To save B. Saved C. Saving D. Having saved

11.O’Neal works hard.He is often seen ___heavily before his teammates start.

A.sweated

B.to be sweated

C.sweating

D.being sweated

12.I can hardly imagine Peter__across the Atlantic Ocean in 15 days. A.sail B.to sail C.sailing D.to have sailed

13.Such___the case, I couldn’t help but _____him.

A.being;support

B.is;to support

C.has been;supporting

D.be;supported

14.Does the way you thought of___the water clean make any sense?

A.making

B.to make

C.how to make

D.having made

15.In order not to be disturbed, I spent three hours__in my study. A.locking B.locked C.to lock D.being locked

16.You will find the word “psychology”____under “P”in your dictionary.

A.have listed

B.list

C.listed

D.listing

17.Sandy could do nothing but ___to his mother that he was wrong.

A.admitting

B.admits

C.admit

D.to admit

18.When I caught him cheating me, I stopped __things in his shop.

A.buying

B.buy

C.to buy

D.bought

19.A doctor can expect___at any hour of the day or night.

A.calling

B.to call

C.being called

D.to be called

20.Nearly every great building in Beijing was built_south.

A.to face

B.facing

C.to have faced

D.being facing

21.With his son___, the old man felt unhappy.

A.to be disappointed

B.disappointing

C.being disappointed

D.to disappoint

22.It was____computer games that cost the boy a lot of time that he ought to have spent on his lessons.

A.to have played

B.playing

C.played

D.having played

23.And there, almost___in the big chair, sat her little brother, who never had to be told to keep quiet.

A.having lost

B.losing

C.to be lost

D.lost

24.As I will be away for at least a year, I’d appreciate__from you now and then__me how everyone is getting along.

A.hearing;tell

B.to hear;tell

C.hearing;telling

D.to hear;to tell

25.The students are forbidden, unless they have special passes, ___after 11 pm.

A.to stay out

B.from staying out

C.staying out

D.not to stay out

26.I worked so late in the office last night that I hardly had time___the last bus.

A.catching

B.to catch

C.having caught

D.to have caught

27.__to sunlight for too much time will do harm to one’s skin.

A.Exposed

B.Having exposed

C.Being exposed

D.After being exposed

28.Mike didn’t start____the importance of a foreign language till he graduated from school.

A.knowing

B.to know

C.know

D.to have known

29.——Why was a special meeting called? ——___a new chairman.

A.To elect

B.Electing

C.Our electing

D.Elected

30.——Why did Bob weep? ——He couldn’t bear___like that before the whole class.

A.making fun of

B.being made fun of

C.to be laughed at

D.being made fun

31.The students expected there___more reviewing classes before the final exams.

A.is

B.to be

C.being

D.have been

32.I don’t mind___by bus, but I hate__in queues.

A.traveling;stand

B.to travel;to stand

C.traveling;to stand

D.traveling;to standing

33.We are looking forward to___the film____at the Grand Cinema.

A.seeing;to show

B.see;shown

C.seeing;shown

D.see;to show

34.At the shopping-centre,he didn’t know what__and__with an empty bag.

A.to buy;leave

B.to be bought;left

C.to buy;left

D.was to buy;leave

35.The policeman put down the phone, ___with a smile on his face.

A.satisfied

B.satisfying

C.to be satisfied

D.having satisfied

36.___, your composition is full of mistakes.

A.Writing carelessly

B.Written carelessl

C.Having written carelessly

D.Being written carelessly

37.She made a candle___us light. A.give B.gave C.to give D.given

38.——What do you suppose made her worried? ——___a gold ring.

A.Lose

B.Lost

C.Losing

D.Because of losing

39.I know it’s not important, but I can’t help __about it.

A.to think

B.and think

C.thinking

D.being thought

40.__several times, the young scientist still kept on making his experiments.

A.Having been failed

B.Having failed

C.Though failed

D.Because of failure

41.___all over the hill and around the lake are wild flowers of different kinds.

A.To grow

B.Growing

C.Grown

D.Grow

42.——Who are you going to have__this letter for you? ——My secretary.

A.type

B.typed

C.been typed

D.been typing

43.The food___at the moment is for the dinner party.

A.cooked

B.to be cooked

C.is being cooked

D.being cooked

44.A kind old gentleman offered___my bags to the taxi stand.

A.his help carried

B.carrying

C.me to carry

D.to help me to carry

45.You can hardly imagine the difficulty the woman had___her children.

A.brought up

B.to bring up

C.bringing up

D.to have brought up

46.He was___today, but was asked to stay for___week.

A.to have returned;another

B.returning;one more

C.returned;another

D.to return;other

47.——Where should I send my form? ——The Personnel office is the place___.

A.for sending it

B.to send it to

C.to send

D.to send it

48.I could feel the wind__on my face from an open window. A.to blow B.blowing C.to be blowing D.blown

49.——Lucy, why didn’t you come last Sunday? ——I___, but my father was in hospital.

A.would

B.had

C.was going to

D.did

50.——What terrible weather! I simply can’t get the car___.

——Why not try____the engine with some hot water?

A. starting;filling

B. start;filling

C. started; to fill

D. to start; fill

答案:

1. D。本题考查独立主格结构,因为前后主语不一致,所以前面分词的逻辑主语不可以省略。It being Sunday相当于As it is Sunday。

2.B。独立主格结构在句中作伴随状语, teeth 与set两个词之间含有逻辑上的被动关系,所以用过去分词;look这一动作是eyes所发出,所以用looking。

3.A。独立主格结构在句中作状语,因为things与consider之间含有被动关系,所以用过去分词。

4.B。分词短语作状语,因为ask与句子的主语(she)之间有被动关系,所以用过去分词。

5.C。本题考查“(only)+不定式”与“-ing形式”作结果状语的区别。“only+不定式”表示该结果在主语的预料之外;接“-ing”形式表示由主句所产生的逻辑上的结果。

6.B。本题考查不定式作目的状语,本题极易误选A。其实enjoy的宾语为fishing or watching TV。

7.B。因finish这一动作发生在I had a drink and went out之前,所以必须用现在分词的完成式。

8.D。介词+关系代词+不定式的结构可以在句中用作定语,其作用相当于一个定语从句。9.B。本题考查非谓语动词作定语。不定式作定语时,表示将来的动作;现在分词作定语,表示主动、进行;过去分词作定语,表示被动、完成。因为dates是被标在gold coin上,所以用过去分词表示被动。

10.A。不定式作目的状语。

11.C。分词在句中作主语补足语。因sweat这一动作是he所发出,所以为主动。

12.C。Peter为逻辑主语,与sailing构成复合结构,作imagine的宾语。

13.A。can’t help but为固定短语, but后面接不带to的不定式;Such being the case为独立主格结构,the case为逻辑主语。14.B。15.B。lock与其逻辑主语应含有动宾关系,即“被锁在书房里”。

16.C。单词psychology被列在“P”之下,有被动关系。

17.C。but表示“除了”,为介词,当其前面有动词do的各种形式时,but后接不带to的不定式。18.A。当stop后面接不定式时,表示“停下来去做某事”;如果接-ing形式,则表示“停止正在做的事”。

19.D。expect后面接不定式。由题意可知,doctor与call之间是被动关系。

20.B。现在分词在句中表示状态。21.B。with复合结构在句中作状语,表示原因,意为“由于儿子很是令人失望。”

22.B。本题为强调句型,被强调的部分是句子的主语,所以采用-ing形式。

23.D。be lost in是固定短语,意为“陷入……之中”。变为分词短语作状语时,仍用过去分词lost。

24.C。appreciate后直接接-ing形式作宾语。25.A。本题考查forbid 的用法。当forbid用作被动形式时,构成be forbidden to do结构。26.B。

27.C。由题意“暴露在阳光下太久,会伤害人的皮肤”可知应采用-ing形式的被动式作主语。28.B。start后可以接-ing形式或to do,但如果start后面的动词表示心理活动的词时,必须用不定式。

29.A。回答why引导的问句,一般用不定式;回答what问句,一般用-ing形式。

30.B。bear意为“容忍”, 后面接-ing形式;make fun of与其逻辑主语有被动关系,所以用being done形式。

31.B。expected there to be的形式相当于expected there would be...。

32.C。mind意为“介意”, 后面接-ing形式;hate后可以接-ing形式或to do两种形式。33.C。look forward to(to为介词)后面接-ing形式;film与show之间含有被动关系,所以用过去分词作定语。

34.C。“特殊疑问词+不定式”结构在句中作宾语;left与didn’t know并列。

35.A。be satisfied with“对……满意”,即satisfy与with紧紧相连时,必须用其过去分词。

36.B。write所表示的动作与其逻辑主语有被动关系。Being written为现在分词进行时的被动,表示该动作正在进行,与题意不符,所以选B。

37.C。不定式作目的状语, 意为“她制了一些蜡烛用来照明”。38.C。参见注29。

39.C。can’t help表示“忍不住”后面接-ing形式;表示“不能帮助”时,后面接不定式。40.B。fail这一动作发生在主句谓语动词之前,所以用现在分词的完成时,并且为主动概念。41.B。all over the hill and around the lake为地点状语提前。wild flowers是长在“山上及湖的周围”的。

42.A。who为have的宾语。43.D。根据句子意思可知该动作正在进行,所以用being done形式。

44.D。本题考查offer与help的用法, 即offer to do sth.与help sb.(to) do sth。45.C。本题考查have difficulty(in) doing sth.这一结构。本句中the woman had为定语从句,修饰difficulty。

46.A。be to+have done结构,表示本应该做某事,但事实上却没有做。

47.B。本题考查不定式作定语的用法。根据send的用法,send sth.to some place,故选择答案B。

48.B。blow这一动作是wind发出的,所以为主动,因此选择blowing。

49.C。该题考查了动词不定式的省略现象。由题意可知是表示过去的一种打算或计划,在“was going to”后省略了前面的内容或动作,故应选C。

50.A。get the car starting意为“使汽车发动起来”;try doing 意为“试着做某事”。

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生化实验思考题参考答案[1].

生化实验讲义思考题参考答案 实验一淀粉的提取和水解 1、实验材料的选择依据是什么? 答:生化实验的材料选择原则是含量高、来源丰富、制备工艺简单、成本低。从科研工作的角度选材,还应当注意具体的情况,如植物的季节性、地理位置和生长环境等,动物材料要注意其年龄、性别、营养状况、遗传素质和生理状态等,微生物材料要注意菌种的代数和培养基成分的差异等。 2、材料的破碎方法有哪些? 答:(1) 机械的方法:包括研磨法、组织捣碎法; (2) 物理法:包括冻融法、超声波处理法、压榨法、冷然交替法等; (3) 化学与生物化学方法:包括溶胀法、酶解法、有机溶剂处理法等。 实验二总糖与还原糖的测定 1、碱性铜试剂法测定还原糖是直接滴定还是间接滴定?两种滴定方法各有何优缺点? 答: 我们采用的是碱性铜试剂法中的间接法测定还原糖的含量。间接法的优点是操作简便、反应条件温和,缺点是在生成单质碘和转移反应产物的过程中容易引入误差;直接法的优点是反应原理直观易懂,缺点是操作较复杂,条件剧烈,不易控制。 实验五粗脂肪的定量测定─索氏提取法 (1)本实验制备得到的是粗脂肪,若要制备单一组分的脂类成分,可用什么方法进一步处理? 答:硅胶柱层析,高效液相色谱,气相色谱等。 (2)本实验样品制备时烘干为什么要避免过热? 答:防止脂质被氧化。 实验六蛋白质等电点测定 1、在等电点时蛋白质溶解度为什么最低? 请结合你的实验结果和蛋白质的胶体性质加以说明。

蛋白质是两性电解质,在等电点时分子所带净电荷为零,分子间因碰撞而聚沉倾向增加,溶液的粘度、渗透压减到最低,溶解度最低。结果中pH约为4.9时,溶液最浑浊,达到等电点。 答: 2、在分离蛋白质的时候,等电点有何实际应用价值? 答: 在等电点时,蛋白质分子与分子间因碰撞而引起聚沉的倾向增加,所以处于等电点的蛋白质最容易沉淀。在分离蛋白质的时候,可以根据待分离的蛋白质的等电点,有目的地调节溶液的pH使该蛋白质沉淀下来,从而与其他处于溶液状态的杂质蛋白质分离。 实验七氨基酸的分离鉴定-纸层析法 1、如何用纸层析对氨基酸进行定性和定量的测定? 答: 将标准的已知氨基酸与待测的未知氨基酸在同一张层析纸上进行纸层析,显色后根据斑点的Rf值,就可以对氨基酸进行初步的定性,因为同一个物质在同一条件下有相同的Rf 值;将点样的未知氨基酸溶液和标准氨基酸溶液的体积恒定,根据显色后的氨基酸斑点的面积与点样的氨基酸质量成正比的原理,通过计算斑点的面积可以对氨基酸溶液进行定量测定。 3、纸层析、柱层析、薄层层析、高效液相层析各有什么特点? 答:

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