卷丹百合黑斑病病原鉴定及生物学特性研究

吴金平;丁自立;刘晓艳;郭凤领;焦忠久;邱正明

【期刊名称】《农业科学与技术（英文版）》

【年(卷),期】2014(000)011

【摘要】随着卷丹百合种植面积的扩大，卷丹百合叶部真菌性病害也越来越严重，尤其在2014年6~7月份，由于降水偏多，卷丹百合叶部病害大发生，6月中旬重病田卷丹百合枯死株率达50%~70%，部分田块卷丹百合地上部全部枯死。从卷丹百合发病叶片分离得到病原菌，进行致病性鉴定、孢子形态观察、18S rDNA序列分析，生物学特性研究，结果表明，该病原菌为半知菌链格孢（ Alternaria alternata）。该菌菌丝生长致死温度为55℃；适宜 pH为6~7；在供试的几种碳、氮源中，最适的碳源是麦芽糖，最适的氮源是酵母。%With the increasing planting area of Lilium lancifolium, the leaf fungal dis-ease of L. lancifolium is becoming more and more serious. In June and July of 2014, the excessive rainfal leads to the serious outbreak of leaf disease of L. lanci-folium. In mid-June, the wilting rate of L. lancifolium in seriously-infected field was even up to 50%-70%. In some fields, the shoots of L. lancifolium even al wilted. The pathogen was isolated from the infected leaves of L. lancifolium. Its pathogenici-ty, spore morphology, 18S rDNA sequence and biological characteristics were stud-ied. The results showed the isolated pathogen was Alternaria alternate. The l ethal temperature of mycelial growth was 55 ℃. The optimum pH was 6-7. Among the tested carbon sources and nitrogen