专题1 2测试题

高三年级选修3 <基因、细胞工程>复习检测

一、选择题（30X1）使用时间：7.28

1．基因工程技术也称DNA 重组技术，其实施必须具备的4个必要条件是 ( )

A ．工具酶、目的基因、运载体、受体细胞

B 、重组DNA 、RNA 聚合酶、内切酶、连接酶

C ．模板DNA 、信使RNA 、质粒、受体细胞

D ．目的基因、限制性内切酶、运载体、体细胞 2．下列关于限制酶的说法正确的是 ( )

A ．限制酶广泛存在于各种生物中，但微生物中很少

B ．一种限制酶只能识别一种特定的核苷酸序列

C ．不同的限制酶切割DNA 后都会形成黏性末端

D ．限制酶的作用部位是特定核苷酸形成的氢键

3．镰刀型细胞贫血症的病因是血红蛋白基因的碱基序列发生了改变。检测这种碱基序列改变必须使用的酶是( )A ．解旋酶 B ．DNA 连接酶 C ．限制酶 D ．RNA 聚合酶

4．由于质粒与目的基因具有相同的黏性末端，结合过程中不．

可能出现下列哪种情况( ) A ．形成环状的外源DNA B ．可能形成环状的载体DNA

C ．可能出现重组DNA

D ．只出现重组DNA

5．下列哪项不．

是基因表达载体的组成部分( ) A ．启动子 B ．终止密码 C ．标记基因 D ．复制原点

6．在基因工程技术中，需要用氯化钙处理的步骤是( )

A ．目的基因的提取

B ．目的基因与载体结合

C ．将目的基因导入受体细胞

D ．目的基因的检测与表达

7．下列叙述符合基因工程概念的是 ( )

A ．

B 淋巴细胞与肿瘤细胞融合，杂交瘤细胞中含有B 淋巴细胞中的抗体基因

B ．将人的干扰素基因重组到质粒后导入大肠杆菌，获得能产生人干扰素的菌株

C ．用紫外线照射青霉菌，使其DNA 发生改变，通过筛选获得青霉素高产菌株

D ．自然界中天然存在的噬菌体自行感染细菌后其DNA 整合到细菌DNA 上

8．不．

属于利用基因工程方法生产的药物是 ( ) A ．干扰素 B ．白细胞介素 C ．青霉素 D ．乙肝疫苗

*9．基因工程中，需使用特定的限制酶切割目的基因和质粒，便于重组和筛选。已知限制

酶Ⅰ的识别序列和切点是—G ↓GATCC —，限制酶Ⅱ的识别序列和切点是—↓

GATC —。根据图示判断下列操作正确的是 ( )

A ．目的基因和质粒均用限制酶Ⅱ切割

B ．目的基因和质粒均用限制酶Ⅰ切割

C ．质粒用限制酶Ⅱ切割，目的基因用限制酶Ⅰ切割

D ．质粒用限制酶Ⅰ切割，目的基因用限制酶Ⅱ切割

10．如果科学家通过转基因工程，成功地把一位女性血友病患者的造血干细胞进行改造，使其凝血功能恢复正常。那么，她后来所生的儿子中 (

) 组号：姓名：组内互评：

A．全部正常B．一半正常C．全部有病D．不能确定

11、科学家们用小鼠骨髓细胞与某种细胞融合，得到杂交瘤细胞，经培养可产生大量的单克隆抗体，与骨髓细胞融合的是（）

A．经过免疫的T淋巴细胞B．未经过免疫的T淋巴细胞

C．经过免疫的B淋巴细胞D．未经过免疫的B淋巴细胞

12、下列黏性末端属于同一种限制酶切割而成的是：（）

A．①② B．①③ C．①④ D．②③

13、如图是“白菜—甘蓝”杂种植株的培育过程。下列说法正确的是

A．图示“白菜—甘蓝”植株不能结籽B．愈伤组织的代谢是自养需氧型

C．上述过程中包含着有丝分裂，细胞分化和减数分裂等过程

D．“白菜—甘蓝”杂种植株具有的性状是基因选择性表达的结果

14、下列有关基因工程的叙述中，不正确的是（）

A.DNA连接酶将黏性末端的碱基对连接起来

B.基因探针是指用放射性同位素或荧光分子等标记的DNA分子

C.基因治疗主要是对有基因缺陷的细胞进行替换

D.蛋白质中氨基酸序列可为合成目的基因提供资料

*15、某同学在学习“细胞工程”时，列表比较了动植物细胞工程的有关内容，你认为有几

植物细胞工程动物细胞工程

技术手段植物组织培养植物体细胞杂交动物细胞培养和融合单克隆抗体制备等特殊处理酶解法去除细胞壁胃蛋白酶处理制细胞悬浮液

融合方法物理方法化学方法生物方法物理方法化学方法生物方法

典型应用无病毒植物的培育、人工种子、

种间植物杂交等

单克隆抗体的制备等

培养基区别所用的糖必须为葡萄糖所用的糖为葡萄糖且动物血清不可缺少

16、植物体细胞杂交的结果是（）

A.产生杂种植株B．产生杂种细胞C．原生质体融合 D．形成愈伤组织

17．下列实验中能体现细胞全能性的是()

①用悬浮培养的胡萝卜单个细胞培养成了可育的植株

②植物用种子进行繁殖后代③用烟草培养的单个组织培育出了可育的完整植株

A．①②B．①③C．②③D．①②③

18．科学家在某种植物中找到了抗枯萎的基因，并以质粒为载体，采用转基因方法培育出了抗枯萎病的金茶花新品种，下列有关说法正确的是( )

A．质粒是最常用的载体之一，它仅存在于原核细胞中 B．将抗枯萎基因连接到质粒上，用到的工具酶仅是DNA连接酶 C．用叶肉细胞作为受体细胞培育出的植株不能表现出抗枯萎性状 D．通过该方法获得的抗枯萎病金茶花，产生的配子不一定含抗枯萎病基因

19．要想通过体细胞杂交技术获得杂种植株，下列各套步骤中正确的是()

A．分离原生质体→诱导原生质体融合→再生细胞壁→组织培养→杂种植株

B．分离原生质体→原生质体自然融合→再生细胞壁→组织培养→杂种植株

C．病毒诱导原生质体融合→组织培养→杂种植株

D．分离原生质体→脱分化→再分化→杂种植株

20．植物体细胞杂交是利用不同物种的两个体细胞融合成一个杂种细胞的过程。该过程是指() A．不同植物的精子和卵细胞的融合B．不同植物的原生质体的融合C．完整的两个体细胞的融合D．植物花粉细胞的两两融合

*21．关于番茄—马铃薯杂种植株，下列叙述不

．正确的是()

A．若番茄细胞内有m条染色体，马铃薯细胞含n条染色体，则“番茄—马铃薯”细胞内含(m＋n)条染色体

B．过程②结束后需要淘汰自身融合的细胞C．过程③不存在细胞分裂

D．这项技术已经广泛应用到杂种植物的培育中

22．一个离体的植物细胞给予适宜的条件后可以经过多次分裂、分化直至发育成一个性成熟的植物。这种技术具有多种用途，但是不．能()

A．有选择地繁殖一种无病毒花卉B．繁殖农作物中的优秀个体

C．拯救濒临绝灭的植物D．改变植物的基因型

23．动物细胞培养中胰蛋白酶所起的作用是()

A．消化细胞膜易于融合B．分解培养基的蛋白质供给细胞营养

C．使组织分散成单个细胞D．用于细胞内物质的分解

24．关于动物细胞培养的叙述错误的是（）

A．用于培养的细胞最好取自健康动物体内的组织细胞

B．将所取的组织先用胰蛋白酶或胶原蛋白酶进行处理其分散成单个细胞，再稀释制成细胞悬液培养C．在培养瓶中要定期用胰蛋白酶或胶原蛋白酶进行处理，然后分瓶继续培养，让细胞继续增殖D．传代培养的细胞在传至10～50代左右时，增殖会逐渐减慢，以至于完全停止，所培养的细胞全部衰老死亡

*25．为获得纯合高蔓抗病番茄植株，采用了下图所示的方法图中两对相对性状独立遗传。据图分析，不正确的是（）

A．过程①的自交代数越多，纯合高蔓抗病植株的比例越高

B．过程②可以取任一植株的适宜花药作培养材料C．过程③包括脱分化和再分化两个过程D．图中筛选过程不改变抗病基因频率

26. 下列哪一项属于克隆()

A．将鸡的某个DNA片段整合到小鼠DNA分子中B．将抗药菌的某基因引入草履虫的细胞内C．将鼠骨髓瘤细胞与经过免疫的脾细胞融合成杂交瘤细胞

D．将某种瘤细胞在体外培养繁殖成一个细胞系

27．以下四种细胞工程技术中，培育出的新个体不．能同时具有两个亲本遗传性状的是（）A．细胞融合B．植物细胞或组织培养C．细胞核移植D．动物胚胎移植

28．有关植物组织培养过程中的细胞脱分化的说法，不正确的是（）A．已经分化的细胞失去特有的结构和功能而转变成未分化的细胞的过程

B．已经分化的细胞转变成为具有分生能力的薄壁细胞，进而形成植物的愈伤组织的过程C．离体的植物组织在一定的培养条件下的分化出根和芽的过程

D．离体的植物组织在脱分化形成愈伤组织的过程中必须经过有关激素的诱导

29．马铃薯利用它的块茎进行无性繁殖，种植的世代多了以后往往会感染病毒而减产，为此农户都希望得到无病毒的幼苗进行种植。获得无病毒幼苗的最佳方法是（）

A．选择优良品种进行杂交B．进行远缘植物体细胞杂交

C．利用芽体进行组织培养D．人工诱导基因突变

*30．将基因型分别为AABb和CCDd的两棵植株的一定数量花粉除去细胞壁后，放入试管中进行原生质体融合（只考虑两个细胞的融合），可以得到不同基因型的融合细胞种类数为（） A、3 B、4 C、6 D、10

二、填空题

*31．根据基因工程的有关知识，回答下列问题：·

（１）限制性内切酶切割ＤＮＡ分子后产生的片段，其末端类型有和。

（２）质粒运载体用EcoRⅠ切割后产生的片段如下：

为使运载体与目的基因相连，含有目的基因的DNA除可用EcoRⅠ切割外，还可用另一种限制性内切酶切割，该酶必须具有的特点是。（３）按其来源不同，基因工程中所使用的DNA连接酶有两类，即DNA连接酶和DNA连接酶。

（４）反转录作用的模板是，产物是。若要在体外获得大量反转录产物，常采用技术。

（５）基因工程中除质粒外，和也可作为运载体。

（６）若用重组质粒转化大肠杆菌，一般情况下，不能直接用未处理的大肠杆菌作为受体细

胞，原因是。

*32．科学家将人的生长素基因与大肠杆菌的DNA分子进行重组，并成功地在大肠杆菌中得以表达。过程如右图据图回答：

(1)人的基因之所以能与大肠杆菌的DNA分子进行重组，原因是

(2)过程①表示的是采取的方法来获取目的基因。其过程可表示为：

_________________________________。

(3)图中(3)过程用人工方法，使体外重

组的DNA分子转移到受体细胞内，主要

是借鉴细菌或病毒___ 细胞的途

径。一般将受体大肠杆菌用

处理，使细胞处于能

______________________的生理状态，

这种细胞称为________细胞，再将表达

载体溶于缓冲液中与____________混

合，在一定的温度下完成转化。

(4)检测大肠杆菌B是否导入了质粒或重组质粒，可采用的方法是___________________

_____________,理由是_______________________________________________________. (5)如果把已经导入了普通质粒A或重组质粒的大肠杆菌，放在含有四环素的培养基上培养，发生的现象是，原因是：。33．如图所示为农作物新品种的育种方式：

(1)图示育种方式涉及到的生物工程技术有：_________________、_________________。

(2)②③过程分别为：________、________。

(3)⑤过程中常用的载体是________；⑤过程表示的基因操作步骤是_________________。

(4)上述育种与传统杂交育种相比，突出优点是_________________________________

________________________________________________________________________。34．科学家从某些能无限增殖细胞的细胞质中分离出无限增殖调控基因(prG)，该基因能

激发许多动物细胞分裂，这为单克隆抗体的制备提供了更为广阔的前景。请回答相关问题：

(1)利用细胞融合技术制备单克隆抗体，促进动物细胞融合常用的诱导因素是________。

(2)利用细胞融合技术制备单克隆抗体时有两次筛选，第1次筛选的目的是____________ _____________________第2次筛选的目的是

_______________________________________

(3)有人提出，可以直接通过基因工程导入该调

控基因来制备单克隆抗体，其思路如图所示。请

回答：

①酶A是指____________________，酶B是指

____________________。

②对已导入重组质粒的Ⅱ进行“检测”的目的是____________________________题目中Ⅱ

所指的细胞是________，Ⅲ所指的细胞具有的特点是__

(4)也有人提出用细胞核移植技术构建重组细胞来生产单克隆抗体，试用相关的图示及文字表示该过程：

35.下图是某淋巴细胞，膜外颗粒状物质为抗体。据图回答：

⑴这是细胞，它是由分化而来的。

⑵抗体从开始合成到分泌出细胞，经过的细胞结构顺序是

（填标号）。抗体的分泌说明细胞的各种生物膜在

结构上具有，并以[ ] 的形式转变

⑶在分泌抗体时要消耗由[ ] 提供的能量。

⑷抗体的化学本质是，它能与发生特异性结

合，发挥免疫效应，该过程属于免疫

⑸若该抗体是单克隆抗体，则该细胞名称为。在制备单克隆抗体的过程中，应用的动物细胞工程的技术手段是。

36．如下图所示为胡萝卜的韧皮部细胞通过无菌操作，接入试管后，在一定的条件下，形成试管苗的培育过程，请根据图回答下列问题：

⑴要促进细胞分裂生长，培养基中应有营养物质和激素。营养物质包括和小分子有机物，激素包括细胞分裂素和两类植物激素。

⑵此过程依据的原理是。A和B阶段主要进行的分裂方式是，B阶段除了细胞分裂外，还进行细胞等。

⑶此过程要无菌操作，主要是指对进行灭菌消毒。B阶段需要光照，原因是。

⑷试管苗的根细胞没有叶绿素，而叶的叶肉细胞具有叶绿素，这是基因

的结果。

问题反馈题号：

Unit 12 单元同步测试题(附答案)

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七下unit11-unit12单元综合测试题姓名________ 班级________ 学号________ 成绩________ 一、单项选择（20分）（）1. —Was there a nice picture on the wall? —______. A．No，there was B．No，there wasn’t C．Yes，there were D．Yes，there wasn’t （）2．There ______ many students in the library after school every day. A．are B．were C．have D．had （）3．______ it rained all day，______we didn’t go to the park. A．Because;/ B．/;because C．/;/ D．Because; so （）4．—Have you ever read the book Harry Potter? —Yes，and I think it’s very ______.I want to read it again. A．boring B．exciting C．bored D．excited （）5．Let’s go ______ a walk after lunch. A．to B．at C．for D．in （）6．—How was your trip? —_______. A．Yes，I don’t like it at all B．What about you? C．Yes，I like it. D．It’s great! （）7．Everything ______ fine yesterday afternoon. A．were B．is C．are D．was （）8．The car ______ and they went out the car. A．stops B．stopped C．stopped D．stop （）9．I’m really ______ about my brother. A．worry B．worries C．worried D．worrys （）10．Could you teach us ______ this game? A．play B．to play C．playing D．that play （）11．It’s easy for him ______ this question. A．answer B．answers C．to answer D．answering （）12．Tina bought a birthday cake ______ May. A．to B．for C．at D．/ （）13．Keep quiet! I can’t _____ the teacher. A．listen B．listened C．heard D．hear （）14. We were ______.The weather was fine all day. A．lucky B．luck C．luckily D．lucks （）15．The farmers _______ the cows ______ grass. A.feed; to B．feed; with C．feeds; on D．feeds; with （）16. —did you go to the mountains？—Last summer. A.Where B.What C.Who D.When （）17. Lucy usually on vacation in Shanghai.But last year she to Guangzhou. A.goes；went B.goes；goes C.went；goes D.went；went （）18. interesting job this is! A.What B.How C.What an D.How an （）19. was the weather like yesterday？

Unit 12 单元测试题

Unit 12 单元测试题听力部分(共20分) ●一、听句子，选择句中所包含的信息。（5分） ( )1. A. put up B. picked up C. put off ( )2. A. box B. sauce C. forks ( )3. A. supposed B. spotted C. stopped ( )4. A. closing B. waiting C. wiping ( )5. A. manners B. makers C. managers ●二、听对话和对话后的问题，选择正确的答案。（5分） ( )6. A. Bow. B. Kiss. C. Shake hands. ( )7. A. To shake hands with the teacher. B. To greet the teacher. C. To talk to the teacher. ( )8. A. To have a rest. B. To prepare for the test at once. C. To ask for others’ help. ( )9. A. Go to a restaurant. B. Do some cooking. C. Have supper. ( )10. A. They’re relaxed about time. B. They are careful with time. C. They hate people who come late. ●三、听对话，根据对话内容判断句子的正（T）误（F）。（5分）( )11. Mary is from America. ( )12. Mary has been in China for three weeks. ( )13. Mary does not know much about China. ( )14. Mary will stay in China for another three months. ( )15. Mary doesn’t get used to Chinese food. ●四、听下面一段材料，并根据材料内容选择正确答案。（5分）( )16. Who is the E-mail sent to? A. Danny. B. Li Shan. C. Danny and Li Shan. ( )17. Why does Danny want to go to the sale? A. The price of books in the store is low. B. The books in the store are free. C. The books in the store are interesting. ( )18. What’s Li Shan’s favorite subject? A. History. B. Computer. C. The arts. ( )19. Will Li Shan go to the bookstore with Danny? A. We don’t know. B. Yes, she will. C. No, she won’t. ( )20. From the passage we know that Danny and Li Shan are ________. A. mother and son B. teacher and student C. friends