常用的PCR Buffer大全

10× PCR buffer Recipe

Solution Volume to

add 10X buffer concentration Working

concentration

1 M KCl 20 ml 500 mM 50 mM

1 M Tris.HCl pH

9.0

4ml 100 mM 10 mM 1%Triton-X 0.4ml 1% 0.1% Sterile ddH2O 15.6 ml Total 40 ml

10× PCR buffer Recipe (GC-rich)

200 mM Tris-HCl (pH8.8 @25℃)

100 mM KCl

100 mM (NH4)2SO4

20 mM MgSO4

1% Triton X-100

1 mg/mL BSA

Long PCR BufferA

5X buffer stock containing 6 mM Mg(OAc)2 (1.2 mM final concentration) and 5% DMSO (1% final concentration) can easily be made using 500 mM Mg(OAc)2, 1 M Tricine and 1 M KOAc stock solutions as follows:

5X Long PCR Buffer

425 mM KOAc

125 mM Tricine (pH 8.7, adjust pH of Tricine stock solution with KOH)

40% Glycerol

5% DMSO

6.0 mM Mg(OAc)2

10 ml 5X buffer

4.25 ml 1M KOAc

1.25 ml 1M Tricine, pH8.7 @ 25 degrees C (with KOH)

4.00 ml Glycerol

0.50 ml DMSO

120 ul 500 mM Mg(OAc)2