TIDE operation manula for TLCS-870C1
瑞士万通870水分测定仪使用原理及常见问题对策
瑞士万通870水分测定仪使用原理及常见问题对策张颖，等瑞"万通870水分测定仪使用原理及常见问题对策The Swiss Wantong870Moisture MeterPrinciple of Use and Common Problems张颖蔡俊泽（河南能源化工集团安化公司,河南安阳455133）［摘要］阐述了瑞士万通870水分测定仪的工作原理，分析在使用过程中仪器出现问题的原因，提出解决方案，并说明在使用过程中的注意事项及日常维护$［关键词］水份仪；测定原理；永停法；故障排除；仪器维护［中图分类号］TH83［文献标识码］B引言河南能源化工集团安化公司（以下简称“安化公司”），前身为河南省安阳化肥厂，项目始建于1968年，1973年建成投产，2009年3月融入河南能源化工集团&主要产品有尿素、复合肥、瓶级聚酯、甲胺/DMF、乙二醇、保险粉等20余种&进入21世纪，我国国民经济高速发展，生产高质量的的产品已成为产业部门的共识，更表明产品质量已成为企业生存与发展的关键，对产品质量监控已成为评价企业信誉的标志。
当前分析已发生化，的工化分析操作方法已逐渐让位给快速、操作简便的仪器分析法。
现在安化公司计量检测管理中心许多分析岗位都在使用瑞士万通最新型容量法卡氏水分测定仪——870精锐plus。
这款新型水分测定能、操作简、能化高，于对有严格要求的化工、医药和包装等行业产品测定&可用于准确分析固体、液体、膏状物样品中游离的，，（0.1%~100%），分析时间短（2~3min）。
但是由于化公司量对的工作原理和使用过程中的注意事项不了解，仪器出，的有，对工的常运行造成了很大影响&针对这种状况，本文总结使用瑞士万通870水分测定仪出现故障时采用的对策和维护措施，以期分享给使用本款仪器的同行，延长仪器使用寿命&1瑞士万通870水分仪测定原理1.1测定原理瑞士万通870水分测定仪是利用卡尔-费休方法测定物质中水分含量的电化学仪器。
辅助试剂说明书 C1 全套小鼠 ELISPOT 辅助试剂盒 说明书
全套小鼠ELISPOT辅助试剂盒ALL-IN-ONE Mouse ELISPOT Accessory Kit说明书Cat#:2230014产品描述：ELISPOT辅助试剂包括：Magi Coating Buffer、Blocking Stock Solution R、Dilution Buffer R、Washing Buffer、AEC显色液。
方便广大ELISPOT客户的使用。
使用前请对照表(1)检查试剂组成成份，若有任何疑问请与深圳市达科为生物工程有限公司联系。
表(1)ELISPOT辅助试剂盒组成使用说明：1.Magi Coating Buffer(1×)：即用型，用于未包被PVDF膜的预湿，用时15μL/孔，预湿1分钟，扣去孔内液体即可进行包被。
2.Blocking Stock Solution R(10×)：封闭缓冲液，用无菌PBS稀释(1:10)即为工作液，用于已包被固相载体上的非特异性结合位点的封闭。
用法：包被完成后，扣去包被液，用无菌PBS洗涤3次(200μL/孔)，以200μL/孔加入封闭缓冲工作液，室温封闭2小时或37°C封闭1小时。
3.Dilution Buffer R(10×)：用1×无菌PBS稀释(1:10)即为工作液，用于生物素标记的抗体(Biotinylated Antibody，Secondary Antibody)及酶联亲和素(Streptavidin-HRP)的稀释。
4.Washing Buffer(50×)：洗涤液，用蒸馏水稀释(1:50)即为工作液，用时260μL/孔，每次洗涤5-7遍。
5.AEC Coloring System：显色系统，用前按顺序取用AEC Dilution、AEC SolutionⅠ(20×)、AECSolutionⅡ(20×)、AEC SolutionⅢ(200×)配制，取用比例为180:10:10:1。
迈勒泰尼生物技术说明书-MACS
.06Miltenyi Biotec B.V. & Co. KGpage 1/4Contents1. Description 1.1 Principle of the MACS® Separation 1.2 Background information 1.3 Applications1.4 Reagent and instrument requirements2. Protocol2.1 Sample preparation 2.2 Magnetic labeling 2.3 Magnetic separation2.4 C ell separation with the autoMACS® Pro Separator3. Example of a separation using CD45 (TIL) MicroBeadsWarningsReagents contain sodium azide. Under acidic conditions sodium azide yields hydrazoic acid, which is extremely toxic. Azide compounds should be diluted with running water before discarding. These precautions are recommended to avoid deposits in plumbing where explosive conditions may develop.1. DescriptionThis product is for research use ponents1 mL CD45 (TIL) MicroBeads, mouse:MicroBeads conjugated to monoclonal anti-mouse CD45 antibodies (isotype: rat IgG2b).CapacityFor 10⁹ total cells, up to 100 separations.Product format CD45 (TIL) MicroBeads are supplied in buffercontaining stabilizer and 0.05% sodium azide.StorageStore protected from light at 2−8 °C. Do not freeze. The expiration date is indicated on the vial label.1.1 Principle of the MACS® SeparationFirst, the CD45+cells are magnetically labeled with CD45 (TIL) MicroBeads. Then, the cell suspension is loaded onto a MACS® Column, which is placed in the magnetic field of a MACS Separator. The magnetically labeled CD45+ cells are retained within the column. The unlabeled cells run through; this cell fraction is thus depleted of CD45+ cells. After removing the column from the magnetic field, the magnetically retained CD45+ cells can be eluted as the positively selected cell fraction. To increase the purity, the positively selected cell fraction containing the CD45+ cells must be separated over a second column.1.2 Background informationCD45 (TIL) MicroBeads, mouse have been developed for theisolation of tumor-infiltrating leukocytes (TILs) from single-cell suspensions of solid mouse tumors. The CD45 antigen is expressed on all cells of hematopoietic origin except erythrocytes and platelets.1.3 Applications●Positive selection of CD45+ leukocytes from solid mouse tumors, e.g., B16F10, 4T1, or CT26.WT.1.4 Reagent and instrument requirements●Buffer: Prepare a solution containing phosphate-buffered saline (PBS), pH 7.2, 0.5% bovine serum albumin (BSA), and 2 mM EDTA by diluting MACS® BSA Stock Solution (# 130-091-376) 1:20 with autoMACS® Rinsing Solution (# 130-091-222). Keep buffer cold (2−8 °C). Degas buffer before use, as air bubbles could block the column. Always use freshly prepared buffer. ▲ Note: EDTA can be replaced by other supplements such as anticoagulant citrate dextrose formula-A (ACD-A) or citrate phosphate dextrose (CPD). BSA can be replaced by other proteins such as mouse serum albumin, mouse serum, or fetal bovine serum (FBS). Buffers or media containing Ca2+ or Mg2+ are not recommended for use.●MACS Columns and MACS Separators: CD45+ cells can be enriched by using MS or LS Columns. Positive selection can also be performed by using the autoMACS Pro or the MultiMACS™ Cell24 Separator.Positive selection MS 10⁷ 2 ×10⁷MiniMACS, OctoMACS, VarioMACS, SuperMACS II LS4 ×10⁷4 ×10⁷5 ×10⁷5 ×10⁷MidiMACS, QuadroMACS, VarioMACS, SuperMACS IIMultiMACS Cell24 Separator PlusautoMACS5 ×10⁷10⁸autoMACS ProMulti-24 Column Block (per column)2 ×10⁷2.5 ×10⁷MultiMACS Cell24 Separator Plus ▲Note: Column adapters are required to insert certain columns into the VarioMACS™ or SuperMACS™ II Separators. For details refer to the respective MACS Separator data sheet. ▲Note: If separating with LS Columns and the MultiMACS Cell24 Separator Plus use the Single-Column Adapter. Refer to the user manual for details.●Tumor Dissociation Kit, mouse (# 130-096-730) for the generation of single-cell suspension from tumor tissues.CD45 (TIL) MicroBeadsmouseOrder no. 130-110-618●gentleMACS™ Dissociator (# 130-093-235), gentleMACS OctoDissociator (# 130-095-937), or gentleMACS Octo Dissociatorwith Heaters (# 130-096-427)●gentleMACS C Tubes (# 130-093-237, # 130-096-334)●(Optional) Fluorochrome-conjugated REA (REAfinity™antibodies: recombinantly engineered, lacking Fcγ-bindingsite) CD45 antibodies for flow cytometric analysis, e.g., CD45-VioBlue®. For more information about antibodies refer to/antibodies.▲Note: Due to expression of Fcγ receptors on tumor-infiltrating leukocytesREA antibodies are recommended.●(Optional) Propidium Iodide Solution (# 130-093-233), DAPIStaining Solution (# 130-111-570), 7-AAD Staining Solution(# 130-111-568), or Viobility™ Fixable Dyes (# 130-109-812,# 130-109-814, # 130-109-816) for flow cytometric exclusion ofdead cells.●(Optional) Dead Cell Removal Kit (# 130-090-101) for thedepletion of dead cells.●(Optional) Pre-Separation Filters (30 µm) (# 130-041-407) toremove cell clumps.●(Optional) MACS SmartStrainers (30 µm) (# 130-098-458) toremove cell clumps.2. Protocol2.1 Sample preparationFor preparation of a single-cell suspension from solid mouse tumors use the Tumor Dissociation Kit, mouse (# 130-096-730) in combination with the gentleMACS™ Dissociators.For details refer to /protocols.▲ Dead cells may bind non-specifically to MACS® MicroBeads. To remove dead cells, we recommend using the Dead Cell Removal Kit (# 130-090-101).2.2 Magnetic labeling▲ Cells can be labeled with MACS MicroBeads using the autolabeling function of the autoMACS® Pro Separator. For more information refer to section 2.4.▲ Work fast, keep cells cold, and use pre-cooled solutions. This will prevent capping of antibodies on the cell surface and non-specific cell labeling.▲ Volumes for magnetic labeling given below are for up to 10⁷ total cells. When working with fewer than 10⁷ cells, use the same volumes as indicated. When working with higher cell numbers, scale up all reagent volumes and total volumes accordingly (e.g. for 2×10⁷ total cells, use twice the volume of all indicated reagent volumes and total volumes).▲ For optimal performance it is important to obtain a single-cell suspension before magnetic labeling. Pass cells through 30 µm nylon mesh (MACS SmartStrainers (30 µm), # 130-098-458). Moisten filter with buffer before use.▲ The recommended incubation temperature is 2–8 °C. Higher temperatures and/or longer incubation times may lead to non-specific cell labeling. Working on ice may require increased incubation times.1. Determine cell number.2. Centrifuge cell suspension at 300×g for 5 minutes. Aspiratesupernatant completely.3. Resuspend cell pellet in 90 µL of buffer per 10⁷ total cells.▲ Note: Always use freshly prepared buffer.4. Add 10 µL of CD45 (TIL) MicroBeads per 10⁷ total cells.5. Mix well and incubate for 15 minutes in the dark in therefrigerator (2−8 °C).6. (Optional) Add staining antibodies according tomanufacturer’s recommendations.7. Add buffer to a final volume of 500 μL for up to 5×10⁷ cells.▲Note: If more cells were used, split the sample onto multiple columns duringmagnetic separation.▲Note: For higher cell numbers, scale up buffer volume accordingly.8.Proceed to magnetic separation (2.3).2.3 Magnetic separation▲ Choose an appropriate MACS Column and MACS Separator according to the number of total cells and the number of CD45+ cells. For details refer to the table in section 1.4.▲ Note: MS Columns are recommended for highest purity of CD45+ cells. LSColumns are recommended for highest recovery of CD45+ cells.▲ For optimal performance it is important to obtain a single-cell suspension before magnetic separation. Pass cells through 30 µm nylon mesh (Pre-Separation Filters (30 µm), # 130-041-407) to remove cell clumps which may clog the column. Moisten filter with buffer before use.▲Always wait until the column reservoir is empty before proceeding to the next step.Magnetic separation with MS or LS Columns1. Place column in the magnetic field of a suitable MACSSeparator. For details refer to the respective MACS Columndata sheet.2. Prepare column by rinsing with the appropriate amount ofbuffer:MS: 500 µL LS: 3 mL3. Apply cell suspension onto the column. Collect flow-throughcontaining unlabeled cells.4. Wash column with the appropriate amount of buffer. Collectunlabeled cells that pass through and combine with theflow-through from step 3.MS: 3×500 µL LS: 2×1 mL▲ Note:Perform washing steps by adding buffer aliquots as soon as the columnreservoir is empty.5. Remove column from the separator and place it on a suitablecollection tube.6. Pipette the appropriate amount of buffer onto the column.Immediately flush out the magnetically labeled cells by firmly pushing the plunger into the column.MS: 1 mL LS: 3 mL7. (Optional) To increase the purity of CD45+cells, the elutedfraction can be enriched over a second MS or LS Column.Repeat the magnetic separation procedure as described in steps 1 to 6 by using a new column.Magnetic separation with the MultiMACS™ Cell24 Separator Refer to the the MultiMACS™ Cell Separator user manual for instructions on how to use the MultiMACS Cell24 Separator.2.4 Cell separation with the autoMACS® Pro Separator▲Refer to the user manual for instructions on how to use the autoMACS® Pro Separator.▲ All buffer temperatures should be ≥10 °C.▲ For appropriate resuspension volumes and cell concentrations, please visit /autolabeling.▲ Place tubes in the following Chill Rack positions:position A = sample, position B = negative fraction,position C = positive fraction.2.4.1 F ully automated cell labeling and separation1. Switch on the instrument for automatic initialization.2. Go to the Reagent menu and select Read Reagent. Scan the2D barcode of each reagent vial with the barcode scanner on the autoMACS® Pro Separator. Place the reagent into the appropriate position on the reagent rack.3. Place sample and collection tubes into the Chill Rack.4. G o to the Separation menu and select the reagent name foreach sample from the Labeling submenu (the correct labeling, separation, and wash protocols will be selected automatically).5. Enter sample volume into the Volume submenu. Press Enter.6. Select Run.2.4.2 M agnetic separation using manual labeling1. Label the sample as described in section2.2 Magnetic labeling.2. Prepare and prime the instrument.3. Apply tube containing the sample and provide tubes forcollecting the labeled and unlabeled cell fractions. Place sample and collection tubes into the Chill Rack.4. For a standard separation choose one the following programs:Positive selection:Posseld2for highest purityorPossels for highest recoveryCollect positive fraction in row C of the tube rack.3. Example of a separation usingCD45 (TIL) MicroBeadsA tumor induced by the B16F10 cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD45+ TILs were isolated from the single-cell suspension using CD45 (TIL) MicroBeads, an MS Column, and a MiniMACS™ Separator.Cells were fluorescently stained with CD45-PE and Labeling-Check-Reagent-VioBlue® and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.Before separation10³-1110¹10²10³10²10¹CD45-PELabelingCheckReagent-VioBlue-11CD45+ cells10³-1110¹10²10³10²10¹CD45-PELabelingCheckReagent-VioBlue-11Refer to for all data sheets and protocols. Miltenyi Biotec provides technical support worldwide. Visit /local to find your nearest Miltenyi Biotec contact.Legal noticesLimited product warrantyMiltenyi Biotec B.V. & Co. KG and/or its affiliate(s) warrant this product to be free from material defects in workmanship and materials and to conform substantially with Miltenyi Biotec’s published specifications for the product at the time of order, under normal use and conditions in accordance with its applicable documentation, for a period beginning on the date of delivery of the product by Miltenyi Biotec or its authorized distributor and ending on the expiration date of the product’s applicable shelf life stated on the product label, packaging or documentation (as applicable) or, in the absence thereof, ONE (1) YEAR from date of delivery (“Product Warranty”). Miltenyi Biotec’s Product Warranty is provided subject to the warranty terms as set forth in Miltenyi Biotec’s G eneral Terms and Conditions for the Sale of Products and Services available on Miltenyi Biotec’s website at , as in effect at the time of order (“Product Warranty”). Additional terms may apply. BY USE OF THIS PRODUCT, THE CUSTOMER AGREES TO BE BOUND BY THESE TERMS.THE CUSTOMER IS SOLELY RESPONSIBLE FOR DETERMINING IF A PRODUCT IS SUITABLE FOR CUSTOMER’S PARTICULAR PURPOSE AND APPLICATION METHODS.Technical informationThe technical information, data, protocols, and other statements provided by Miltenyi Biotec in this document are based on information, tests, or experience which Miltenyi Biotec believes to be reliable, but the accuracy or completeness of such information is not guaranteed. Such technical information and data are intended for persons with knowledge and technical skills sufficient to assess and apply their own informed judgment to the information. Miltenyi Biotec shall not be liable for any technical or editorial errors or omissions contained herein.All information and specifications are subject to change without prior notice. Please contact Miltenyi Biotec Technical Support or visit for the most up-to-date information on Miltenyi Biotec products.LicensesThis product and/or its use may be covered by one or more pending or issued patents and/or may have certain limitations. Certain uses may be excluded by separate terms and conditions. Please contact your local Miltenyi Biotec representative or visit Miltenyi Biotec’s website at for more information.The purchase of this product conveys to the customer the non-transferable right to use the purchased amount of the product in research conducted by the customer (whether the customer is an academic or for-profit entity). This product may not be further sold. Additional terms and conditions (including the terms of a Limited Use Label License) may apply.CUSTOMER’S USE OF THIS PRODUCT MAY REQUIRE ADDITIONAL LICENSES DEPENDING ON THE SPECIFIC APPLICATION. THE CUSTOMER IS SOLELY RESPONSIBLE FOR DETERMINING FOR ITSELF WHETHER IT HAS ALL APPROPRIATE LICENSES IN PLACE. Miltenyi Biotec provides no warranty that customer’s use of this product does not and will not infringe intellectual property rights owned by a third party. BY USE OF THIS PRODUCT, THE CUSTOMER AGREES TO BE BOUND BY THESE TERMS.TrademarksautoMACS, gentleMACS, MACS, MACSQuant, MidiMACS, the Miltenyi Biotec logo, MiniMACS, MultiMACS, OctoMACS, QuadroMACS, REAfinity, SuperMACS, VarioMACS, Viobility, and VioBlue are registered trademarks or trademarks of Miltenyi Biotec and/or its affiliates in various countries worldwide.Copyright © 2020 Miltenyi Biotec and/or its affiliates. All rights reserved.。
TLC Pharmaceutical Standards 15(S)-Latanoprost Saf
SAFETY DATA SHEETPrint date December4,2019Section1.IdentificationsProduct name:15(S)-LatanoprostTLC ID:L-322Product Use:For laboratory use only.Not for use in humans or animals,drug,household or other use.Supplier/Manufacturer:TLC Pharmaceutical Standards Ltd.130Pony DriveNewmarket,ON,L3Y7B6,CanadaTelephone:(905)-898-3645Fax:(905)-898-0595Website:Section2.Hazards identificationsPhysical state:LiquidWarning:Harmful if swallowed,inhaled or in contact with skin.Routes of entry:Inhalation,skin,eyesGHS classification:Skin irritation(Category2)Serious eye damage/eye irritation(Category2)Acute toxicity,oral(Category4)Acute toxicity,dermal(Category4)Acute toxicity,inhalation(Category4)GHS Label elements,including precautionary statementsPictogram(s):Signal word:WarningHazards statements:H302+H312+H332Harmful if swallowed,in contact with skin or if inhaled.H315Causes skin irritation.H319Causes serious eye irritation.Precautionary statements:P261Avoid breathing dust/fume/gas/mist/vapours/spray.P271Use only outdoors or in a well-ventilated area.P280Wear protective gloves/protective clothing/eye protection/face protection.P302+P352IF ON SKIN:wash with plenty of soap and water.P305+P351+P338IF IN EYES:Rinse cautiously with water for several minutes.Removecontact lenses,if present and easy to do.Continue rinsing.position/Information on IngredientsCAS No.:145773-22-4Molecular Formula:C26H40O5Molecular Weight:432.60Synonyms:Isopropyl(Z)-7-((1R,2R,3R,5S)-3,5-dihydroxy-2-((S)-3-hydroxy-5-phenylpentyl)cyclopentyl)hept-5-enoateSection4.First-aid measuresGeneralConsult a physician.Show this safety data sheet to the doctor in attendance.Move out of dangerous area.If inhaledIf breathed in,move person into fresh air.If not breathing,give artificial respiration.Consult a physician.In case of eye contactRinse thoroughly with plenty of water for at least15minutes and consult a physician.In case of skin contactWash off with soap and plenty of water.Consult a physician.If swallowedDo NOT induce vomiting.Never give anything by mouth to an unconscious person.Rinse mouth with water.Consult a physician.Section5.Firefighting measuresConditions of flammabilityNot flammable or combustible.Suitable extinguishing mediaUse water spray,alcohol-resistant foam,dry chemical or carbon dioxide.Hazardous combustion productsHazardous decomposition products formed under fire conditions:carbon oxides,sodium oxides,nitrogen oxides Special firefighting proceduresWear self-contained breathing apparatus and protective clothing.Section6.Accidental release measuresPersonal precautionsWear respiratory protection.Avoid dust formation.Avoid breathing vapours,mist or gas.Ensure adequate ventilation.Evacuate personnel to safe areas.Avoid breathing dust.Environmental precautionsPrevent further leakage or spillage if safe to do so.Do not let product enter drains.Method and materials forcontainment and cleaning upMethod and materials for containment and cleaning upPick up and arrange disposal without creating dust.Sweep up and shovel.Keep in suitable,closed containers for disposal.Section7.Handling and storageHandlingAvoid contact with skin and eyes.Avoid formation of dust and aerosols.Provide appropriate exhaust ventilation at places where dust is formed.Keep away from sources of ignition.Take measures to prevent the buildup of electrostatic charge.StorageKeep refrigerated as specification of Certificate of Analysis.Keep container tightly closed in a dry and well-ventilated place.Section8.Exposure controls/personal protectionEngineering controlsUse mechanical exhaust or laboratory fumehood to avoid exposure.Personal protective equipmentRespiratory protectionWhere risk assessment shows air-purifying respirators are appropriate use a full-face respirator with multi-purpose combination(US)or type ABEK(EN14387)respirator cartridges as a backup to engineering controls.If the respirator is the sole means of protection,use a full-face supplied air e respirators and components tested and approved under appropriate government standards such as NIOSH(US)or CEN(EU)Hand ProtectionHandle with chemical-resistant gloves,solvent-resistant gloves.Gloves must be inspected prior to use.Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices.Wash and dry hands.Eye ProtectionFace shield and safety glasses Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH(US)or EN166(EU).Skin and body protectionComplete suit protecting against chemicals,flame retardant antistatic protective clothing.The type of protective equipment must be selected according to the concentration and amount of the dangerous substance at the specific workplace.Section9.Physical and chemical propertiesPhysical property:LiquidColour:ColorlessOdour:No data availableDensity:No data availableMelting Point/Freezing Point(°C):No data availableFlash Point(°C):No data availableExplosive Properties:No data availableOxidizing Properties:No data availableWater solubility:No data availableSolubility(other solvents):Methanol,AcetonitrileSection10.Stability and reactivityReactivity:No data available.Chemical stability:Stable under recommended storage conditions.Conditions to avoid:Heat,flames and sparks.Extremes of temperature and direct sunlight.Materials to avoid:oxidizing agents.Hazardous decomposition products:possible products formed under fire conditions:Carbon oxides.Section11.Toxicological informationAcute toxicityOral LD50:No data available.Inhalation.LC50:No data available.Dermal LD50:No data available.Skin Corrosion/Irritation:Toxic if absorbed through skin.May cause skin irritation.Eyes:May cause eye irritation.Acute and Chronic Health hazards:TLV:None verified.Effects of Overexposure:May causes eye,respiratory,and skin irritation.May be harmful by inhalation,ingestion,or skin absorption.To the best of our knowledge,the chemical,physical,and toxicological properties have not been thoroughly investigated Section12.Ecological informationNo data available.Section13.Disposal considerationsProductBurn in a chemical incinerator equipped with an afterburner and scrubber but exert extra care in igniting as this material is highly flammable.Offer surplus and non-recyclable solutions to a licensed disposal company.Contact a licensed professional waste disposal service to dispose of this material.Contaminated packagingDispose of as unused product.Section14.Transport informationNon-hazardous for transport.IATA-Not regulatedSection15.Regulatory informationTLV:None verifiedSection16.Other informationThis product is not bioactive,not radioactive.It is for R&D use only.Not for drug,household or other uses.The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide.TLC Pharmaceutical Standards Ltd.extends no warranties with respect hereto and disclaims all liabilities from reliance thereon.All judgments as to the suitability of the data presented with respect to the use of this product are theresponsibility of the purchaser and intended user.。
欧洲药典7.5版
INDEX
To aid users the index includes a reference to the supplement in which the latest version of a text can be found. For example : Amikacin sulfate...............................................7.5-4579 means the monograph Amikacin sulfate can be found on page 4579 of Supplement 7.5. Note that where no reference to a supplement is made, the text can be found in the principal volume.
English index ........................................................................ 4707
Latin index ................................................................................. 4739
EUROPEAN PHARMACOPபைடு நூலகம்EIA 7.5
Index
Numerics 1. General notices ................................................................... 7.5-4453 2.1.1. Droppers...................
FLOCKLOK 870 产品说明书
USA SAFETY DATA SHEET3000010022401. CHEMICAL PRODUCT AND COMPANY IDENTIFICATIONProduct name: FLOCKLOK 870 Product Use/Class:Flock AdhesiveLORD Corporation 111 LORD DriveCary, NC 27511-7923 USATelephone: 814 868-3180Non-Transportation Emergency: 814 763-2345 Chemtrec 24 Hr Transportation Emergency No.800 424-9300 (Outside Continental U.S. 703 527-3887)EFFECTIVE DATE: 04/05/20192. HAZARDS IDENTIFICATIONGHS CLASSIFICATION:Flammable liquids Category 2Acute toxicity Inhalation - Vapours Category 4 - 55.6% of the mixture consists of ingredient(s) of unknown toxicity. Acute toxicity Inhalation - Dust and Mist Category 3 - 55.6% of the mixture consists of ingredient(s) of unknown toxicity.Skin corrosion/irritation Category 2Serious eye damage/eye irritation Category 2A Skin sensitization Category 1Respiratory sensitization Category 1 Carcinogenicity Category 2Reproductive toxicity Category 2Specific target organ systemic toxicity (single exposure) Category 1 Respiratory system, Central nervous system, Kidney, LiverSpecific target organ systemic toxicity (single exposure) Category 3Specific target organ systemic toxicity (repeated exposure) Category 2 Ears, LiverSpecific target organ systemic toxicity (repeated exposure) Category 1 Respiratory system, Body, Central nervous system, Kidney, Nervous systemHazardous to the aquatic environment - acute hazardCategory 2 Hazardous to the aquatic environment - chronic hazardCategory 2GHS LABEL ELEMENTS:Symbol(s)Signal WordD ANGERHazard StatementsHighly flammable liquid and vapor. Toxic if inhaled.Causes skin irritation.Causes serious eye irritation.May cause an allergic skin reaction.May cause allergy or asthma symptoms or breathing difficulties if inhaled. Suspected of causing cancer.Suspected of damaging fertility or the unborn child. May cause harm to breast-fed children.Causes damage to organs.(Respiratory system, Central nervous system, Kidney, Liver)May cause drowsiness or dizziness.May cause respiratory irritation.May cause damage to organs through prolonged or repeated exposure.(Ears, Liver)Causes damage to organs through prolonged or repeated exposure.(Respiratory system, Body, Central nervoussystem, Kidney, Nervous system)Toxic to aquatic life.Toxic to aquatic life with long lasting effects.Precautionary StatementsPreventionKeep away from heat/sparks/open flames/hot surfaces. - No smoking.Ground/Bond container and receiving equipment.Use explosion-proof electrical/ventilating/lighting equipment.Use only non-sparking tools.Take precautionary measures against static discharge.Obtain special instructions before use.Do not handle until all safety precautions have been read and understood.Wear protective gloves/protective clothing/eye protection/face protection.Use personal protective equipment as required.In case of inadequate ventilation wear respiratory protection.Do not breathe dust/fume/gas/mist/vapors/spray.Wash thoroughly after handling.Do not eat, drink or smoke when using this product.Use only outdoors or in a well-ventilated area.Contaminated work clothing should not be allowed out of the workplace.Avoid release to the environment.ResponseIn case of fire: refer to section 5 of SDS for extinguishing media.Call a POISON CENTER or doctor/physician.Specific treatment (see supplemental first aid instructions on this label).IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing.IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower.If skin irritation or rash occurs: Get medical advice/attention.IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do.Continue rinsing.Collect spillage.StorageStore in a well-ventilated place. Keep cool.Store in a well-ventilated place. Keep container tightly closed.Store locked up.Disposal:Dispose of contents/container in accordance with waste/disposal laws and regulations of your country or particular locality.Other Hazards:This product contains component(s) which have the following warnings; however based on the GHS classification criteria of your country or locale, the product mixture may be outside the respective category(s).Acute: Vapor harmful; may affect the brain or nervous system causing dizziness, headache or nausea. Possibleirritation of the respiratory system can occur causing a variety of symptoms such as dryness of the throat, tightness of the chest, and shortness of breath. May cause central nervous system depression characterized by the followingprogressive steps: headache, dizziness, staggering gait, confusion, unconsciousness or coma. May cause lungdamage. Animal tests have indicated that respiratory sensitization can result from skin contact with certainisocyanates.May be harmful if swallowed. Ingestion is not an expected route of entry in industrial or commercial uses.Chronic: May cause long-term lung damage. May affect the gastrointestinal system. May affect the blood andblood-forming organs. Prolonged or repeated contact may result in dermatitis. Ethylbenzene has been classified byIARC as a possible human carcinogen (Group 2B) and reported by NTP to show clear evidence for carcinogenicity in animals. IARC has designated Methyl isobutyl ketone to be in Group 2B - possibly carcinogenic to humans.3. COMPOSITION/INFORMATION ON INGREDIENTSChemical Name CAS Number RangeMethyl isobutyl ketone108-10-115 - 20%Toluene108-88-310 - 15%Xylene1330-20-7 5 - 10%Ester solvent PROPRIETARY 1 - 5%Ethyl benzene100-41-4 1 - 5%4,4'-Diphenylmethane diisocyanate101-68-8 1 - 5%2,6-Di-tert-butyl-p-cresol128-37-00.1 - 0.9%Any "PROPRIETARY" component(s) in the above table is considered trade secret, thus the specific chemical and its exact concentration is being withheld.4. FIRST AID MEASURESFIRST AID - EYE CONTACT: Flush eyes immediately with large amount of water for at least 15 minutes holding eyelids open while flushing. Get prompt medical attention.FIRST AID - SKIN CONTACT: Flush contaminated skin with large amounts of water while removing contaminated clothing. Wash affected skin areas with soap and water. Get medical attention if symptoms occur.FIRST AID - INHALATION: Move person to fresh air. Restore and support continued breathing. If breathing is difficult, give oxygen. Get immediate medical attention.FIRST AID - INGESTION: If swallowed, do not induce vomiting. Call a physician or poison control center immediately for further instructions. Never give anything by mouth if victim is rapidly losing consciousness, unconscious or convulsing.5. FIRE-FIGHTING MEASURESSUITABLE EXTINGUISHING MEDIA: Carbon Dioxide, Dry Chemical, Foam, Water FogUNSUITABLE EXTINGUISHING MEDIA: Not determined for this product.SPECIFIC HAZARDS POSSIBLY ARISING FROM THE CHEMICAL: Flammable liquid and vapor. Keep containers tightly closed. Isolate from heat, electrical equipment, sparks, open flame, and other sources of ignition. Closed containers may rupture when exposed to extreme heat. Use water spray to keep fire exposed containers cool. During a fire, irritating and/or toxic gases and particulate may be generated by thermal decomposition or combustion.SPECIAL PROTECTIVE EQUIPMENT AND PRECAUTIONS FOR FIRE-FIGHTERS: Wear full firefighting protective clothing, including self-contained breathing apparatus (SCBA). Water spray may be ineffective. If water is used, fog nozzles are preferable.6. ACCIDENTAL RELEASE MEASURESPERSONAL PRECAUTIONS, PROTECTIVE EQUIPMENT AND EMERGENCY PROCEDURES: Remove all sources of ignition (flame, hot surfaces, and electrical, static or frictional sparks). Avoid contact. Avoid breathing vapors. Use self-contained breathing equipment.ENVIRONMENTAL PRECAUTIONS: Do not contaminate bodies of water, waterways, or ditches, with chemical or used container.METHODS AND MATERIALS FOR CONTAINMENT AND CLEANUP: Keep non-essential personnel a safe distance away from the spill area. Notify appropriate authorities if necessary. Avoid contact. Before attempting cleanup, refer to hazard caution information in other sections of the SDS form. Contain and remove with inert absorbent material.7. HANDLING AND STORAGEHANDLING: Keep closure tight and container upright to prevent leakage. Ground and bond containers when transferring material. Avoid skin and eye contact. Wash thoroughly after handling. Avoid breathing of vapor or spray mists. Do not handle until all safety precautions have been read and understood. Empty containers should not be re-used. Use with adequate ventilation. Because empty containers may retain product residue and flammable vapors, keep away from heat, sparks and flame; do not cut, puncture or weld on or near the empty container. Do not smoke where this product is used or stored.STORAGE: Do not store or use near heat, sparks, or open flame. Store only in well-ventilated areas. Do not puncture, drag, or slide container. Keep container closed when not in use. Refer to OSHA 29CFR Part 1910.106 "Flammable and Combustible Liquids" for specific storage requirements.INCOMPATIBILITY: Amines, acids, water, hydroxyl, or active hydrogen compounds.; Strong acids, bases, and strong oxidizers.8. EXPOSURE CONTROLS/PERSONAL PROTECTIONCOMPONENT EXPOSURE LIMITChemical Name ACGIH TLV-TWA ACGIH TLV-STELOSHA PEL-TWAOSHA PEL-CEILINGSkinMethyl isobutyl ketone50 ppm75 ppm410 mg/m3100 ppmN.E.N.A.Toluene20 ppm N.E.200 ppm 300 ppmN.A.Xylene100 ppm150 ppm435 mg/m3100 ppmN.E.N.A. Ester solvent N.E.N.E.N.E. N.E.N.A.Ethyl benzene20 ppm N.E.435 mg/m3100 ppmN.E.N.A.4,4'-Diphenylmethane diisocyanate 0.005 ppm N.E.N.E. 0.2 mg/m30.02 ppmN.A.2,6-Di-tert-butyl-p-cresol 2 mg/m3N.E.N.E. N.E.N.A.N.A. - Not Applicable, N.E. - Not Established, S - Skin DesignationEngineering controls: Sufficient ventilation in pattern and volume should be provided in order to maintain air contaminant levels below recommended exposure limits. Caution: Solvent vapors are heavier than air and collect in lower levels of the work area. Sufficient ventilation (using explosion-proof equipment) should be provided to prevent flammable vapor/air mixtures from accumulating.PERSONAL PROTECTION MEASURES/EQUIPMENT:RESPIRATORY PROTECTION: Use a NIOSH approved chemical/mechanical filter respirator designed toremove a combination of particulates and organic vapor if occupational limits are exceeded. For emergencysituations, confined space use, or other conditions where exposure limits may be greatly exceeded, use an approved air-supplied respirator. This product contains isocyanates which have poor odor warning properties. If occupational exposure limits are exceeded, a NIOSH approved supplied-air respirator is required. For respirator use observeOSHA regulations (29CFR 1910.134) or use in accordance with applicable laws and regulations of your country or particular locality.SKIN PROTECTION: Use neoprene, nitrile, or rubber gloves to prevent skin contact.EYE PROTECTION: Use safety eyewear including safety glasses with side shields and chemical goggles where splashing may occur.OTHER PROTECTIVE EQUIPMENT: Use disposable or impervious clothing if work clothing contamination is likely. Remove and wash contaminated clothing before reuse.HYGIENIC PRACTICES: Wash hands before eating, smoking, or using toilet facility. Do not smoke in any chemical handling or storage area. Food or beverages should not be consumed anywhere this product is handled or stored. Wash thoroughly after handling.9. PHYSICAL AND CHEMICAL PROPERTIESTypical values, not to be used for specification purposes.ODOR: Solvent VAPOR PRESSURE: N.D.APPEARANCE: Clear VAPOR DENSITY: Heavier than Air PHYSICAL STATE: Liquid LOWER EXPLOSIVE LIMIT: 1 %(V)UPPER EXPLOSIVE LIMIT: 13.1 %(V)FLASH POINT: 61 °F, 16 °C SetaflashClosed CupBOILING RANGE: 111 - 146 °C EVAPORATION RATE: Slower than n-butyl-acetate AUTOIGNITION TEMPERATURE:N.D.DENSITY: 0.97 g/cm3 - 8.10 lb/gal DECOMPOSITION TEMPERATURE:N.D. VISCOSITY, DYNAMIC: ≥40 mPa.s @ 25 °C ODOR THRESHOLD: N.D.VISCOSITY, KINEMATIC: ≥41 mm2/s @ 25 °CSOLUBILITY IN H2O: Insoluble VOLATILE BY WEIGHT: 44.89 %pH: N.A.VOLATILE BY VOLUME: 52.14 %FREEZE POINT: N.D. VOC CALCULATED: 3.63 lb/gal, 435 g/l COEFFICIENT OF WATER/OILN.D.DISTRIBUTION:LEGEND: N.A. - Not Applicable, N.E. - Not Established, N.D. - Not Determined10. STABILITY AND REACTIVITYHAZARDOUS POLYMERIZATION: Hazardous polymerization will not occur under normal conditions. STABILITY: Product is stable under normal storage conditions.CONDITIONS TO AVOID: High temperatures. Sources of ignition.INCOMPATIBILITY: Amines, acids, water, hydroxyl, or active hydrogen compounds.; Strong acids, bases, and strong oxidizers.HAZARDOUS DECOMPOSITION PRODUCTS: Carbon monoxide, carbon dioxide, Monomeric isocyanate, traces of hydrogen cyanide, nitrogen dioxide11. TOXICOLOGICAL INFORMATIONEXPOSURE PATH: Refer to section 2 of this SDS.SYMPTOMS:Refer to section 2 of this SDS.TOXICITY MEASURES:Chemical Name LD50/LC50Methyl isobutyl ketone Oral LD50: Rat2,080 mg/kgDermal LD50: Rabbit3,000 mg/kgInhalation LC50: Rat8.2 mg/l/4 hToluene Oral LD50: Rat2,600 mg/kgDermal LD50: Rabbit12,000 mg/kgInhalation LC50: Rat12.5 mg/l/4 hXylene Oral LD50: Rat3,500 mg/kgDermal LD50: Rabbit> 4,350 mg/kgInhalation LC50: Rat29.08 mg/l/4 hEster solvent Oral LD50: Rat8,532 mg/kgDermal LD50: Rabbit> 5 g/kgEthyl benzene Oral LD50: Rat3,500 mg/kgDermal LD50: Rabbit15,400 mg/kgInhalation LC50: Rat17.4 mg/l/4 h4,4'-Diphenylmethane diisocyanate Oral LD50: Rat31,600 mg/kgDermal LD50: rabbit> 5,000 mg/kgGHS LC50 (vapour): Acute toxicity point estimate11 mg/l/4 h GHS LC50(dust and mist): Acute toxicity point estimate1.5 mg/l/4 h 2,6-Di-tert-butyl-p-cresol Oral LD50: Rat> 2,930 mg/kgDermal LD50: Rat> 2,000 mg/kgGerm cell mutagenicity: No classification proposedCarcinogenicity: Category 2 - Suspected of causing cancer.Components contributing to classification: Methyl isobutyl ketone. Ethyl benzene.Reproductive toxicity: Category 2 - Suspected of damaging fertility or the unborn child. May cause harm to breast-fed children.Components contributing to classification: Toluene. Xylene. Ethyl benzene. 2,6-Di-tert-butyl-p-cresol.12. ECOLOGICAL INFORMATIONECOTOXICITY:Chemical Name EcotoxicityMethyl isobutyl ketone Fish: Pimephales promelas496 - 514 mg/l96 h flow-throughInvertebrates: Daphnia magna170 mg/l48 hPlants: Pseudokirchneriella subcapitata400 mg/l96 hToluene Fish: Pimephales promelas15.22 - 19.05 mg/l96 h flow-throughPimephales promelas12.6 mg/l96 h StaticOncorhynchus mykiss5.89 - 7.81 mg/l96 h flow-throughOncorhynchus mykiss14.1 - 17.16 mg/l96 h StaticOncorhynchus mykiss5.8 mg/l96 h semi-staticLepomis macrochirus11.0 - 15.0 mg/l96 h StaticOryzias latipes54 mg/l96 h StaticPoecilia reticulata28.2 mg/l96 h semi-staticPoecilia reticulata50.87 - 70.34 mg/l96 h StaticInvertebrates: Daphnia magna5.46 - 9.83 mg/l48 h StaticDaphnia magna11.5 mg/l48 hPlants: Pseudokirchneriella subcapitata> 433 mg/l96 hPseudokirchneriella subcapitata12.5 mg/l72 h StaticXylene Fish: Pimephales promelas13.4 mg/l96 h flow-throughOncorhynchus mykiss2.661 - 4.093 mg/l96 h StaticOncorhynchus mykiss13.5 - 17.3 mg/l96 hLepomis macrochirus13.1 - 16.5 mg/l96 h flow-throughLepomis macrochirus19 mg/l96 hLepomis macrochirus7.711 - 9.591 mg/l96 h StaticPimephales promelas23.53 - 29.97 mg/l96 h StaticCyprinus carpio780 mg/l96 h semi-staticCyprinus carpio> 780 mg/l96 hPoecilia reticulata30.26 - 40.75 mg/l96 h StaticInvertebrates: water flea3.82 mg/l48 hGammarus lacustris0.6 mg/l48 hEster solvent Fish: Pimephales promelas161 mg/l96 h StaticInvertebrates: Daphnia magna> 500 mg/l48 hEthyl benzene Fish: Oncorhynchus mykiss11.0 - 18.0 mg/l96 h StaticOncorhynchus mykiss4.2 mg/l96 h semi-staticPimephales promelas7.55 - 11 mg/l96 h flow-throughLepomis macrochirus32 mg/l96 h StaticPimephales promelas9.1 - 15.6 mg/l96 h StaticPoecilia reticulata9.6 mg/l96 h StaticInvertebrates: Daphnia magna1.8 - 2.4 mg/l48 hPlants: Pseudokirchneriella subcapitata4.6 mg/l72 hPseudokirchneriella subcapitata> 438 mg/l96 hPseudokirchneriella subcapitata2.6 - 11.3 mg/l72 h StaticPseudokirchneriella subcapitata1.7 - 7.6 mg/l96 h Static4,4'-Diphenylmethane diisocyanate Fish: Species> 1,000 mg/l96 hInvertebrates: Daphnia magna> 1,000 mg/l48 h2,6-Di-tert-butyl-p-cresol Plants: Pseudokirchneriella subcapitata6 mg/l72 hDesmodesmus subspicatus> 0.42 mg/l72 hPERSISTENCE AND DEGRADABILITY:Not determined for this product.BIOACCUMULATIVE: Not determined for this product.MOBILITY IN SOIL: Not determined for this product.OTHER ADVERSE EFFECTS: Not determined for this product.13. DISPOSAL CONSIDERATIONSDISPOSAL METHOD: Disposal should be done in accordance with Federal (40CFR Part 261), state and local environmental control regulations. If waste is determined to be hazardous, use licensed hazardous waste transporter and disposal facility.14. TRANSPORT INFORMATIONUS DOT RoadProper Shipping Name: AdhesivesHazard Class: 3SECONDARY HAZARD: NoneUN/NA Number: 1133Packing Group: IIEmergency Response Guide Number: 128IATA CargoPROPER SHIPPING NAME: AdhesivesHazard Class: 3HAZARD CLASS: NoneUN NUMBER: 1133PACKING GROUP: IIEMS: 3LIMDGPROPER SHIPPING NAME: AdhesivesHazard Class: 3HAZARD CLASS: NoneUN NUMBER: 1133PACKING GROUP: IIEMS: F-EThe listed transportation classification applies to non-bulk shipments. It does not address regulatory variations due to changes in package size, mode of shipment or other regulatory descriptors. For the most accurate shipping information, refer to your transportation/compliance department.15. REGULATORY INFORMATIONU.S. FEDERAL REGULATIONS: AS FOLLOWS:SARA SECTION 313This product contains the following substances subject to the reporting requirements of Section 313 of Title III of the Superfund Amendment and Reauthorization Act of 1986 and 40 CFR part 372.:Chemical Name CAS Number Weight % Less ThanMethyl isobutyl ketone108-10-120.0%Toluene108-88-315.0%Xylene1330-20-710.0%Ethyl benzene100-41-4 5.0%4,4'-Diphenylmethane diisocyanate101-68-8 5.0%TOXIC SUBSTANCES CONTROL ACT:INVENTORY STATUSThe chemical substances in this product are on the TSCA Section 8 Inventory.EXPORT NOTIFICATIONThis product contains the following chemical substances subject to the reporting requirements of TSCA 12(B) if exported from the United States:None16. OTHER INFORMATIONUnder HazCom 2012 it is optional to continue using the HMIS rating system. It is important to ensure employees have been trained to recognize the different numeric ratings associated with the HazCom 2012 and HMIS schemes.HMIS RATINGS - HEALTH: 2* FLAMMABILITY: 3 PHYSICAL HAZARD: 1* - Indicates a chronic hazard; see Section 2Revision: New GHS SDS FormatEffective Date: 04/05/2019DISCLAIMERThe information contained herein is, to the best of our knowledge and belief, accurate. However, since the conditions of handling and use are beyond our control, we make no guarantee of results, and assume no liability for damages incurred by use of this material. It is the responsibility of the user to comply with all applicable federal, state and local laws and regulations.。
TIDE-HVAC02-TM-00 DLV车间AHCU-5C HVAC系统验证追溯矩阵 TM_RA_DQ_IQ_OQ
北京泰德制药股份有限公司验证文件编号：TIDE-HV AC02-TM-00DLV车间AHCU-5C HV AC系统验证追溯矩阵起草人：日期年月日审核人：日期年月日审核人：日期年月日审核人：日期年月日批准人：日期年月日目录1. 目的 (2)2. 范围 (2)3. 验证追溯矩阵表 (2)4. 验证追溯结论 (4)1. 目的本文件目的主要是对系统的风险评估确定的验证活动和验证（DQ、IQ及OQ）测试内容之间的联系进行总结。
可追溯性矩阵指出风险评估所确定的验证活动已经过测试。
2. 范围本次验证对象为AHCU-5C HV AC系统，本系统主要用于DLV车间产品的洗瓶、轧盖等生产，该生产区域的房间洁净级别为D级。
3. 验证追溯矩阵表RA DQ IQ OQ编号关键部件验证活动编号内容编号内容编号内容8.2.1 空调机组DQ对风量风压设计、冷却加热能力、过滤器配置、机组材料结构等进行检查；IQ对机组表面及组装情况进行确认；OQ对房间温湿度进行确认。
9.4HVAC系统的设计确认9.4外观质量检查确认9.7温湿度测试确认8.2.2风管DQ对风管尺寸、布置、送排风口布置及数量等进行检查；IQ对风管进行外观及检漏，对安装情况进行确认。
9.4HVAC系统的设计确认9.49.5外观质量检查确认风管制作、清洗、安装、检漏认N/A N/A8.2.3风阀IQ对风阀安装情况进行确认；OQ进行风量及压差调节确认。
9.4HVAC系统的设计确认9.4外观质量检查确认9.59.6风量测试确认压差测试确认8.2.1 高效过滤器DQ对高效设计的过滤效率及DOP测试设计等进行检查；IQ进行检漏。
9.5高效过滤器的设计确认9.6过滤器检漏确认N/A N/A8.2.5仪表 IQ进行校准确认。
N/A N/A 9.7 仪表校准确认N/A N/A8.2.6 控制部件仪表DQ进行仪表及功能设计确认；IQ进行控制柜9.4HVAC系统的设计9.8控制系统和电气柜9.5 风量测试确认及电气安装确认、I/O 测试；OQ进行风量、温湿度、压差、洁净度确认测试。
检测他汀类药物代谢基因多态性的引物及探针组合物、试剂盒、方法
专利名称：检测他汀类药物代谢基因多态性的引物及探针组合物、试剂盒、方法
专利类型：发明专利
发明人：刘福平,刘晶晶,郑建权
申请号：CN202010052082.X
申请日：20200117
公开号：CN111154861A
公开日：
20200515
专利内容由知识产权出版社提供
摘要：本发明涉及检测他汀类药物代谢基因多态性的引物及探针组合物、试剂盒及检测方法，该检测他汀类药物代谢基因多态性的引物及探针组合物，包括以下至少一对引物及探针组合物：用于检测SLCO1B1位点的第一引物和第一探针；用于检测APOE位点的第二引物和第二探针；用于检测ABCB1位点的第三引物和第三探针；以及用于检测LDLR位点的第四引物和第四探针。
本发明的检测他汀类药物代谢基因多态性的试剂盒具有检测全面、通量高、操作简单、检测迅速且安全无污染的优点。
申请人：深圳会众生物技术有限公司
地址：518000 广东省深圳市宝安区航城街道三围社区索佳科技园综合大楼9层A901号
国籍：CN
代理机构：深圳市博锐专利事务所
代理人：汤星星
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用汇编语言编写的应用程序，只需要Memory部分的描述。 Memory部分的描述是对存储器的用途作划分描述。 例：TMP89FM42UG的存储区描述 memory { SFR data SFR3 SFR2 code.m CALLV_VECTOR INT_VECTOR } 说明： －只有在LCF文件中有描述的存储区，才能分配数据。 －因此，在LCF文件中描述的存储区大小可以小于目标 MCU的存储区的实际大小，但绝不能有逾越。
Ø新建或添加源程序
删除则选中不需要的文件按键盘的“Delete”键。
仅
参考 供
鼠标点中工程文件，单击右键，在下拉菜单中选择新建或者添加源程序。
注意：添加的源程序文件最好存放在此工程区下。 TOSHIBA ELECTRONICS SHENZHEN CO.,LTD.
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2010.2.11 Frank Huang
注意：1. 安装路径不能包含中文字符。 2. 以前安装过 T870C 文件的，一定要把此文件卸载掉。 3. 安装文件后面的数字，比如 V170 仅表示软件版本号 V1.70。
TOSHIBA ELECTRONICS SHENZHEN CO.,LTD.
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三、TIDE的使用
仅
ØCPU家族和Memory类型的选择
参考 供
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仅
参考 供
新建的项目
TOSHIBA ELECTRONICS SHENZHEN CO.,LTD.
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参考 供
东芝 TLCS-870/C1开发工具的使用
---TIDE Feb / 2010
TOSHIBA ELECTRONICS SHENZHEN CO.,LTD.
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仅
参考 供
一、TIDE的简介
东芝公司开发的单片机开发工具TIDE（Toshiba Integrated Development Environment）是一个窗口化得软件开发平台，它集成 了功能强大的文本编辑器、工程管理器、各种编译工具（包括C编译 器、宏汇编器、链接定位器、十六进制文件转换器等）以及调试器为 一体的软件开发环境，并支持实时调试操作系统，广泛应用于东芝 8 位到16位单片机的开发。
TOSHIBA ELECTRONICS SHENZHEN CO.,LTD.
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参考 供
: : : : : : :
org=0x0000 , len=0x0040 org=0x0040 , len=0x0800 org=0x0e40 , len=0x00c0 org=0x0f00 , len=0x0100 org=0x8000 , len=0x7fa0 org=0xffa0 , len=0x0020 org=0xffcc, len=0x0034
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参考 供
Rebuild：编译所有的源文件
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2010.2.11 Frank Huang
仅
参考 供
双击红色显示的错误信息可以打 开错误所在的源文件以便修改。
编译失败，无法生成目标文件 *.abs 文件
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如何在代码中找寻密码设置
仅
参考 供
Password count storage address：
0xFF00
Password comparison start address：0xC000 Password：C83A4800B1F034684E3F02
§ 调试操作说明
命令 Go Stop Reset Reset and Go Step Source Line Step Instruction Next Source Line Next Instruction Finish Function Skip Source Line Skip Instruction Run to Here Move to Here Stop Debugging 说明 运行程序 停止运行程序 复位 复位后运行程序 基于源程序的单步运行 基于机器指令的单步运行 运行下一条源程序语句 运行下一条机器指令语句 运行完整个函数 基于源程序的单步跳过 基于机器指令的单步跳过 PC指针运行到此处 PC指针直接跳到此处 退出调试
编译器常用选项：
仅
参考 供
代码优化等级
编译出错警告等级
输出汇编阶段的调试信息 至ABS文件，实现源代码 级调试，即在调试阶段能 观察到源代码。
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2010.2.11 Frank Huang
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参考 供
生成MAP文件。在MAP文 件里可以看到所有数据在 存储器中的分配地址。
Ø芯片ROM区操作
仅
参考 供
每次加载程序时，都把更新的程序 写入芯片内。
每次写芯片之前先执行擦除操作。
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2010.2.11 Frank Huang
Ø中断开关
仅
参考 供
调试过程中禁止/允许中断响应。
单步运行过程禁止/允许中断响应。
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Ø设置编译操作选项设定
打开主菜单[Project] à [Setting]，选中项目名。
仅
参考 供
在其中可以设定编译器、连接器、目标代码转换器、绝对地址编辑器等的所有功能。
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二、TIDE的软件安装
1. 解压文件 “SW00mn0-zccv170”;
仅
参考 供
2. 然后在生成的文件中点击安装文件 “Toshiba IDE” 进行安装，默认安装路径 “C:\Program Files\Toshiba\IDE”。 3. 安装好IDE后，在点击 “sw89cn3-zccv110.exe” 文件进行安装 TLCS870C,C1 系列的C编译器。编译器默认安装路径 “C:\Program Files\Toshiba\T870C” 在 编译器的安装路径下有一个名为 BIN的目录，该目录下的文件如 Cc870c.exe (870/C,C1编译器)、Asm870c.exe(870/C,C1的汇编器)、Tulink.exe 等工具， TIDE对源程序的编译、汇编、链接等操作都是调用这些工具来实现的。Ø编辑/修Fra bibliotek源程序仅
参考 供
双击文件名，在右边的文本区进行 编辑修改。
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2010.2.11 Frank Huang
Ø新建LCF文件
仅
参考 供
在编译之前，必须要建立 LCF 文件
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禁止/允许外部复位。
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Ø调试系统自动载入待调试的目标文件
仅
参考 供
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Ø新项目的创建
主菜单[File] à [New]
注意 件 ：文 路径
参考 供
符 文字 。
含中 能包 不
项目名
项目存放路径 新项目 同时建立工作区
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参考 供
设定编译器的路径。 -- 指定到刚才安装的编译 器 T870C 文件路径下。
设定CPU家族/系列 8bit – 86: 870C 87: 870 88: 870X 89: 870C1 16bit – 91: 900L1 93: 900L 95: 900H 32bit – 92: 900H1
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Ø仿真器类型选择
仅
参考 供
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Ø芯片型号选择
仅
参考 供
Next
Ø调试窗口
调试工具栏
仅
参考 供
当前PC指针 源文件显示 窗口
信息日志窗口
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