7 PCR143F120-0606S Final Inspection Report2010.4.6
pcr仪合格证书

pcr仪合格证书
PCR 仪合格证书是用于确认一台聚合酶链式反应(PCR)仪符合特定标准和要求的文件。
以下是对PCR 仪合格证书的介绍:
1. 证明仪器性能:PCR 仪合格证书是由相关权威机构或实验室颁发的,用于证明该仪器在一系列性能测试中达到了规定的标准。
这些测试通常包括温度控制准确性、升温速率、循环时间等关键参数的评估。
2. 确保实验结果可靠:合格证书的存在意味着该PCR 仪已经通过了严格的测试和验证程序,能够提供稳定和可靠的实验结果。
它为用户提供了一种信任和保证,即该仪器在进行基因扩增等实验时能够准确地执行预期的反应条件。
3. 合规性要求:在某些地区或行业,使用PCR 仪进行实验可能需要符合特定的法规或标准。
PCR 仪合格证书可以作为仪器符合这些要求的证据,帮助用户满足合规性要求。
4. 质量保证:合格证书是PCR 仪制造商对其产品质量的承诺。
它表示该仪器在设计、制造和测试过程中遵循了严格的质量控制标准,以确保其性能和可靠性。
PCR 仪合格证书对于确保仪器的性能、实验结果的可靠性以及合规性
要求的满足至关重要。
它为用户提供了一种信任和保证,使他们能够放心地使用该仪器进行基因扩增等实验。
Odyssey CLX 操作指南1

导入图片 ........................................................................................................................... 18 泳道设置 ........................................................................................................................... 19 设定 Marker....................................................................................................................... 20 创建新 marker ................................................................................................................... 20 自动识别条带 ................................................................................................................... 21 手动编辑条带 ................................................................................................................... 21 单通道信号归一化 ..............................................................................................................21 查看表格 ........................................................................................................................... 22
东洋纺快速Taq HS染料混合物使用手册 101盒说明书

manual Quick Taq HS Dye Mix 1306 F1138K Quick Taq HS DyeMixDTM-101 100 reactionsStore at -20°C Contents[1] Introduction[2] Components[3] Primer design[4] Analysis and Cloning of PCR products[5] Protocol1. Standard reaction setup2. Cycling conditions[6] Examples[7] TroubleshootingCAUTIONAll reagents in this kit are intended for research purposes. Do not use for diagnosis or clinical purposes. Please observe general laboratory precaution and utilize safety while using this kit.JAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio1JAPAN CHINA TOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140 www.toyobo.co.jp/bio/********************[ 1 ] Introduction[ 2 ] Components[ 3 ] Primer Design[ 4 ] Analysis andCloning of PCR productsDescriptionQuick Taq HS DyeMix is a Taq-based 2× master mix PCR reagent that contains anelectrophoresis dye (BPB; bromophenol blue) and anti-Taq antibodies for hot start PCR. This reagent contains all components for PCR except primers and template DNA. This reagent shows specific and efficient amplification. The amplified products can be directly loaded in the wells of agarose or acrylamide gels.Features-As this reagent contains bromophenol blue (BPB) as an electrophoresis dye; the PCR products can be analyzed directly with an agarose or acrylamide gel.-This reagent exhibits greater PCR performance than conventional rTaq DNA polymerase.-This reagent contains anti-Taq antibodies for hot start PCR. Hot start technology realizes highly specific and sensitive PCR.-This reagent is stable for at least three months at 4°C. No decrease in reaction efficiency is observed following 30 freeze-thaw cycles.-This reagent is suitable for a colony-direct PCR (see [6], Example 2).This reagent includes the following components for 100 reactions, 50 μl total reaction volume:2× Quick Taq HS DyeMix1.25ml× 2*In the case of the long-term storage (>3 months), this reagent should be stored at-20°C.Primers should be 22–35 bases long, with a melting temperature (Tm) > 60°C.-As this reagent contains bromophenol blue (BPB) as an electrophoresis dye and by adjusting its relative density, the PCR products can be applied directly to an agarose or acrylamide gel.-The PCR products can be cloned using general TA cloning technology.-The PCR products can be used as templates for sequencing after an appropriate treatment.JAPAN CHINA TOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140 www.toyobo.co.jp/bio/********************[ 5 ] Protocol[ 6 ] Examples1. Standard reactionBefore preparing the reaction mixture, the master mix solution should be completely thawed. *For the PCR reaction, thin-wall tubes are recommended. A total reaction volume of 50 μl is also recommended.2. PCR cycle conditionsExample 1. Amplification of the human p53 gene (2.9 kb)The human p53 gene (2.9 kb) was amplified using 50 ng of human genomic DNA.Quick Taq HS DyeMix successfully amplified the targets.Reaction volume 50 μl 20 μl Final concentrationAutoclaved, distilled water X μl X μl 2x Quick Taq HS DyeMix 25 μl 10 μl 1 × 10 pmol /μl Primer #1 1.0 μl 0.4 μl 0.2 μM 10 pmol /μl Primer #2 1.0 μl 0.4 μl 0.2 μM Template DNAY μlY μlGenomic DNA -200 ng / 50 μl Plasmid DNA -50 ng / 50 μl E. coli colonyTotal 50 μl 20 μl3-step cyclePredenaturation: 94°C , 2min. Denaturation: 94°C , 30sec. °C , 30sec. Extension: 68°C , 1min. /kb 2-step cyclePredenaturation: 94°C , 2min. Denaturation: 94°C , 30sec. Extension: 68°C , 1min. /kbJAPAN CHINA TOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140 www.toyobo.co.jp/bio/********************[ 7 ] Trouble shootingExample 2. Insert amplification by a colony-direct PCRThe inserts were amplified using Quick Taq HS DyeMix with universal primers from E. coli DH5α colonies bearing pTA2 plasmid (insert size: 500 bp). Quick Taq HS DyeMix successfully and efficiently amplified all targets.SymptomCauseSolutionNo PCR product / low yieldCycling conditions are not suitable.Increase the number of cycles by 2-5 cycles. Primer is not good.Check the quality of the primers. Redesign the primers.Template DNA is ofinsufficient quality and/or quantity.Check the quality of the template DNA. Increase the amount of the template DNA Too much sample Excessive amounts of bacterial cells may inhibit amplification. Decrease the sample volume. Smearing / Extra bandCycling conditions are not suitable.Decrease the number of cycles by 2-5 cycles.Primer concentration is not appropriate Optimize the primer concentration to around 0.1-0.2 μM.Annealing temperature is too low. Optimize the annealing temperature to around 55°C -65°C . Primer is not good. Check the quality of the primers. Redesign the primers. Too much template DNA Reduce the amount of template DNA。
QPCR及QRT-PCR系列产品

Invitrogen的ICFC系列产品促销1.QPCR及QRT-PCR系列产品Invitrogen公司专门为中国客户提供的定量PCR试剂盒,结合了 UDG 防止残余污染技术和SYBR® Green I 荧光染料(存在于SYBR® Green I荧光定量PCR试剂盒中),在美国接受了严格的质量监控,可提供极高灵敏度的目的序列定量检测,线性剂量低,反应浓度范围很大。
qPCR Supermix-- 即用型反应剂,专为高特异性、实时定量DNA扩增设计UDG-- 防止携带污染物,减少克隆片段假阳性结果ROX参考染料-- 适用ABI仪器的校正染料产品信息活动时间:即日起至2009年4月30日2.Gibco南美胎牛血清即日起凡优惠价¥1780购买Gibco胎牛血清500ml(目录号:C2027050)即可获赠送价值¥250现金抵用券。
您可以凭现金抵用券在英韦创津公司购买任何商品,此券有效期至2009年5月31日。
产品信息活动时间:即日起至2009年4月30日独特的采集方式:GIBCO采用无菌心脏穿刺的方式采血原装直送,避免污染:原产地采集、加工、检测、包装。
完善的质控:采集、处理、检测、运输等环节都有文件和证书。
3.Invitrogen TA Cloning克隆产品专门用于克隆Taq聚合酶扩增的PCR产物。
采用pCR载体,能产生80%以上的重组产物,90%以上重组产物都包含插入片段。
产品信息活动时间:即日起至2009年5月31日附:pCR载体优点及图谱:3’-T突出端可直接连接Taq扩增的PCR产物可选择T7或T7和Sp6启动子进行体外RNA转录和测序侧向EcoRⅠ位点的通用多接头位点方便了插入片段的切离可以选择卡那霉素或氨苄青霉素进行筛选非常简便的蓝/白克隆筛选具有M13正向和反向引物位点,方便测序4.GIBCO液体培养基系列产品创立近50年的历史,品质优秀,产品种类丰富;为了中国用户利益,特建立国内生产线;所有产品,从原材料到生产全部按照GIBCO质量标准进行,每批均送抵美国公司总部质检合格后,才在国内销售。
ASM 142系列蜜芬泄漏检测器说明书

G B 00207 - E d i t i o n 05 - F e b r u a r y 121/1A 100Introduction to the ASM 142 seriesA new generation of adixen helium leakdetectorModel photographed: ASM 142The ASM 142/142 D/142 S/ASM Graph/ASM Graph D/ASM Graph D+ are universal helium leak detectors which set new performance standards for multi-purpose unit.These detectors are the end-result of an innovative engineering approach utilizing the latest electronics technologies and vacuum concepts, whichmake them a truly universal unit:142I high performances, such as, a roughing capacity of 7 CFM (60 l/mn) with a usable helium sensitivity in the 10-11 /sec range.x x xI high performances, such as, a dry roughing capacity of 0.9 CFM (1.5 m 3/h) with a usable helium sensitivity in the 10-11 /sec range.x x xI a usable helium sensitivity in the10-7 /sec range (with auto-zero function).xI comprehensive control panel with two distinct areas (one for the operation of the unit, the other for entering the test parameters).x x x x x xI evolved features to assist the operator in his daily operation (auto-calibration, auto-zero, helium signal direct readout, ...).x x x x x xI very rugged design, based on field-proven components, which makes it ideal for any industrial environment.x x x x x xI various accessories to reinforce the versatility of the product (remote control, sniffer probe).x x x x x xI totally dry leak detector.x xxI specific to sniffing test mode applications.xI graphic interface.xxxWe suggest that you read this manual before you start to use your detector to obtain optimum levels of performance and complete satisfaction.adixen Vacuum Products - ASM 142 S Operating instructions。
艾本德pcr故障代码手册

艾本德pcr故障代码手册(实用版)目录1.概述2.艾本德 PCR 故障代码手册的主要内容3.艾本德 PCR 故障代码手册的使用方法4.艾本德 PCR 故障代码手册的优点5.总结正文1.概述艾本德 PCR 故障代码手册是一本针对艾本德 PCR 仪器故障排除的专业指南。
PCR(聚合酶链式反应)是一种生物技术方法,常用于扩增 DNA 分子。
艾本德作为一家知名的生物技术企业,其生产的 PCR 仪器在实验中可能会遇到各种故障。
为了帮助用户快速准确地解决这些问题,艾本德推出了这本故障代码手册。
2.艾本德 PCR 故障代码手册的主要内容艾本德 PCR 故障代码手册包含了众多故障代码及其对应的解决方案。
这些代码涵盖了从仪器启动、样本准备到数据分析等各个环节可能出现的问题。
每个故障代码都对应一个唯一的数字,方便用户快速查询。
同时,手册中还提供了详细的故障现象描述和故障排除步骤,以便用户根据实际情况进行操作。
3.艾本德 PCR 故障代码手册的使用方法在使用艾本德 PCR 故障代码手册时,用户应首先确定仪器出现的故障现象,然后根据现象在手册中查找对应的故障代码。
接着,根据代码所提供的解决方案,进行相应的操作,以排除故障。
若无法解决,可尝试联系艾本德技术支持获取进一步的帮助。
4.艾本德 PCR 故障代码手册的优点艾本德 PCR 故障代码手册具有以下优点:(1)针对性强:该手册专门针对艾本德 PCR 仪器设计,内容紧密贴合实际应用场景。
(2)易用性高:手册采用数字编码和分步骤解决方案,方便用户快速查询和操作。
(3)专业性强:手册中的内容由艾本德公司的专业技术团队编写,具有较高的专业水平和权威性。
5.总结艾本德 PCR 故障代码手册是一本实用的专业工具书,对于使用艾本德 PCR 仪器的用户来说,具有很高的参考价值。
SureSelect Target Enrichment RNA Reagent Kit - HSQ

SureSelect Target Enrichment RNA Reagent Kit - HSQ *************(24小时)化学品安全技术说明书GHS化学品标识应急咨询电话(带值班时间)::供应商/ 制造商:安捷伦科技(上海)有限公司中国(上海)外高桥自由贸易试验区英伦路412号(邮编:200131)电话号码: 800-820-3278传真号码: 0086 (21) 5048 2818SureSelect Target Enrichment RNA Reagent Kit - HSQ化学品的推荐用途和限制用途5190-4396 / 5190-4404 / 5190-44125190-4397 / 5190-4405 / 5190-4413SureSelect Hyb 45190-4398 / 5190-4406 / 5190-4414SureSelect Binding Buffer 5190-4399 / 5190-4407 / 5190-4415SureSelect Wash Buffer 15190-4400 / 5190-4408 / 5190-4416SureSelect Wash Buffer 25190-4401 / 5190-4409 / 5190-4417SureSelect Elution Buffer 5190-4402 / 5190-4410 / 5190-4418SureSelect Neutralization Buffer 5190-4403 / 5190-4411 / 5190-4419SureSelect RNase Block 5190-4383 / 5190-4386SureSelect Hyb 35190-4382 / 5190-4385SureSelect Block 25190-4381 / 5190-4384SureSelect Indexing Block 15190-4427 / 5190-4428SureSelect Indexing Block 35190-4445 / 5190-4446SureSelect ILM Indexing Post Capture Forward PCR Primer5190-4441 / 5190-4442SureSelect ILM Index Pre Capture PCR Reverse Primer5190-4443 / 5190-4444SureSelect Adaptor Oligo Mix 5190-4932 / 5190-3619SureSelect Primer 5190-4933 / 5190-3620PCR Primer Index 1-16各种各样的*部件号:物质用途:0.4 ml(毫升) - 12 ml(毫升)0.096 ml(毫升) - 1.25 ml(毫升)SureSelect Hyb 40.208 ml(毫升) - 6.25 ml(毫升)SureSelect Binding Buffer 13.2 ml(毫升) - 400 ml(毫升)SureSelect Wash Buffer 18 ml(毫升) - 240 ml(毫升)SureSelect Wash Buffer 224 ml(毫升) - 720 ml(毫升)SureSelect Elution Buffer0.96 ml(毫升) - 29 ml(毫升)SureSelect Neutralization Buffer 0.96 ml(毫升) - 29 ml(毫升)SureSelect RNase Block 0.016 ml(毫升) - 0.096 ml(毫升)SureSelect Hyb 30.16 ml(毫升) - 0.96 ml(毫升)SureSelect Block 20.045 ml(毫升) - 0.24 ml(毫升)SureSelect Indexing Block 10.045 ml(毫升) - 0.24 ml(毫升)SureSelect Indexing Block 30.012 ml(毫升) - 0.058 ml(毫升)SureSelect ILM Indexing Post Capture Forward PCR Primer0.045 ml(毫升) - 0.27 ml(毫升)SureSelect ILM Index Pre Capture PCR Reverse Primer0.045 ml(毫升) - 0.27 ml(毫升)SureSelect Adaptor Oligo Mix 1.2 ml(毫升) (16 反应)SureSelect Primer0.3 ml(毫升) (16 反应)PCR Primer Index 1-160.0125 ml(毫升) - 0.025 ml(毫升)部件号(化学品试剂盒):G9601A, G9601B, G9601C 注解 *:* PCR Primer Index 1-16: 5190-3021, 5190-3037, 5190-3022, 5190-3038, 5190-3023,5190-3039, 5190-3024, 5190-3040, 5190-3025, 5190-3041, 5190-3026, 5190-3042,5190-3027, 5190-3043, 5190-3028, 5190-3044, 5190-3029, 5190-3045, 5190-3030,5190-3046, 5190-3031, 5190-3047, 5190-3032, 5190-3048, 5190-4423, 5190-4468,5190-4424, 5190-4469, 5190-4425, 5190-4470, 5190-4426, 5190-4471安全技术说明书根据 GB/ T 16483-2008 和 GB/ T 17519-2013物质或混合物的分类根据 GB13690-2009 和 GB30000-2013紧急情况概述SureSelect Hyb 1液体。
安捷伦产品目录

15
Real-Time PCR
16
Mx3000P QPCR System
17
Brilliant III Ultra-Fast SYBR Green QPCR and QRT-PCR Reagents
18
Brilliant III Ultra-Fast QPCR and QRT-PCR Reagents
Agilent / STRATAGENE
Agilent website: /genomics
Welgene | Agilent Stratagene
威健股份有限公司 | Stratagene 總代理
Table of Content
Table of Contents
/ XL1-Red Competent Cells SoloPack Gold Supercompetent Cells
/ TK Competent Cells Specialty Cells
/ Classic Cells / Fine Chemicals For Competent Cells
適用於 UNG 去汙染或 bisulphite
sequencing
適用於 TA Cloning
最高敏感性
取代傳統 Taq 的好選擇
-
2
威健股份有限公司 | Stratagene 總代理
PCR Enzyme & Instrument
Agilent SureCycler 8800
市場上領先的 cycling 速度和 sample 體積 10 ~ 100 μL 簡易快速可以選擇 96 well 和 384 well 操作盤 優秀的溫控設備讓各個 well 都能保持溫度的穩定 七吋的高解析度觸控螢幕讓操作上更為簡便 可以透過網路遠端操控儀器及監控儀器 Agilent 專業的技術支援可以幫助您應對各種 PCR 的問題