RPN Intergrated Test VII (A)

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诺其-更胜一筹的旁路制剂——rFVIIa与PCC的10大不同

诺其-更胜一筹的旁路制剂——rFVIIa与PCC的10大不同
50-100 U/kg,每次使用劑量勿超過100 U/kg單日劑量最高不得超過200 U/kg3
注射用重组人凝血因子VIIa说明书.人凝血酶原复合物说明书-山东泰邦生物制品有限公司.“Baxter” FEIBA说明书.王兆钺, 凝血酶原复合物的临床应用. Chinese Journal of Thrombosis and Hemostasis 2008;14(5):238.
2.6-48OxfordUnits/mL
两组间无显著性差异
两个家庭在治疗失败后拒绝继续使用hFVIII,随后研究停止
5
Gompertset al.
RCT
Proplex 75 U/kg,Proplex SX 75 U/kg,最多2剂
21/51
膝、踝、腕或肘关节急性出血
≥2BU/mL
63%1剂,22%2剂给药后止血,17%需要其他治疗
1996~2013年,超过4,000,000次标准剂量 (90 µg/kg体重)注射
rFVIIa安全性数据——18年,仅169例病人发生TE
适应证
病人数≥1 TE
动脉血栓性事件数
静脉血栓性事件数
混合血栓性事件数*
血栓性事件总数
血友病伴抑制物
73
13
37
34
84
获得性血友病
50
21
12
21
54
先天性FVII缺乏症
2.FVIIa血药浓度不同
rFVIIa规格:1 mg (50,000 IU)/支复溶1 mL含1 mg/50,000 IU 1
PCC规格:300 IU/20 mL/瓶复溶20 mL含四种凝血因子2
品名
推荐用量
总量
rFVIIa

Rhinogen

Rhinogen

产品说明书Rhinogen®胰蛋白酶(测序级)Rhinogen® Trypsin (Sequencing Grade)重组生产,不含动物源性成分,纯度高,比活高,适用于蛋白质组学研究中肽谱图、肽指纹谱或蛋白序列分析产品货号:QIP-003-A,QIP-003-B目录产品信息 (2)试剂包装 (2)产品来源 (2)产品质量 (2)产品特性 (2)保藏条件 (2)产品综述 (3)背景 (3)概述 (3)应用 (3)特性 (3)操作方法 (4)试剂准备 (4)推荐使用方法 (4)操作说明 (4)相关产品 (5)参考文献 (6)产品文档及支持 (7)产品文档 (7)技术支持 (7)试剂包装Rhinogen® Trypsin (Sequencing Grade)包装规格如下:目录号规格QIP-003-A 20μgQIP-003-B 5×20μg产品来源Rhinogen® Trypsin (Sequencing Grade)是利用重组大肠杆菌系统表达生产并经过多步层析纯化得到的重组胰蛋白酶,其氨基酸序列与猪源胰蛋白酶序列同源,分子量大小约为25kDa。

产品质量SDS-PAGE分析,纯度≥95%;没有检测到污染的蛋白酶活性。

产品特性最适pH为7.0~8.0;比活(单位/mg)≥4500USP units/mg。

保藏条件采用冰袋运输,收到产品后请立即将酶置于-20℃,密封防潮。

使用50mM HAc或1mM HCl溶解后,-70℃存放;若溶解后存于2-8℃,需在24hr内使用完毕。

避免室温下长时间放置,避免反复冻融。

背景胰蛋白酶(Trypsin)属于丝氨酸蛋白酶家族,能特异性切割多肽链中赖氨酸和精氨酸残基中的羧基。

胰蛋白酶原由胰腺分泌,受肠激酶或胰蛋白酶的限制分解成为活化胰蛋白酶,是肽链内切酶。

它不仅起消化酶的作用,而且还能限制分解糜蛋白酶原、羧肽酶原、磷脂酶原等其它酶的前体,起活化作用,是特异性最强的蛋白酶。

海伦娜血小板聚集仪aggRAM新建Worklist步骤

海伦娜血小板聚集仪aggRAM新建Worklist步骤

Agg RAM TM血小板聚集仪新建Worklist步骤运行仪器控制软HemoRam后,点击file→worklist或图标入工作列表窗口在Worklist ID输入工作列表名称(如ADP+AA)Test Type栏中从下拉菜单中选择Platelet Aggregation血小板聚集检测,Test栏选择要做的诱导剂检测。

(如Test栏选择Adenosine Diphosphate则4个通道默认为Adenosine Diphosphate诱导剂检测血小板聚集;带*号的是组合检测,可以根据所组合项目选择*Screen2或3)Stir Speed 为磁珠转数,默认为600rpm。

每个通道可以在Reagent 栏中从下拉菜单选择不同的诱导剂,可将默认的诱导剂修改实际要选择的诱导剂类型,单项的不可修改。

Patient ID 栏输入要做检测的病人ID 号,如不填则仪器认为该通道不检测样本。

Unit of Measure 栏为所选择的诱导剂的浓度单位。

Concentration 栏为所选择的诱导剂的浓度。

Lot Number 栏为所选择的诱导剂的批号信息,可不填。

点击Save ,保存工作列表信息。

注意:每次在Worklist 中修改的诱导剂检测组合,并在Patient ID 输入了要检测标本的病人ID 号,则所做的组合检测项目修改将保存在工作列表中。

如在Patient ID 栏不输入病人ID 号,则下次在选择该Worklist 时,则将恢复为默认的诱导剂检测组合。

下表为各诱导剂的浓度单位和最终浓度试剂名称二磷酸腺苷肾上腺素胶原花生四稀酸花生四稀酸(2倍稀释)缩写ADP EPN COLL ACA ACA 最终浓度5umol /L75umol /L (0.075mmol/L )2.5ug /ml500ug /ml (0.5mg /ml))250ug /ml (0.25mg /ml))本文由心我飞羊根据aggAM 仪器操作手册编制,发布于百度文库,仅提供给仪器操作人员参考。

P1噬菌体转导敲除基因的实验步骤

P1噬菌体转导敲除基因的实验步骤

P1噬菌体转导基因敲除操作步骤实验室常用大肠杆菌基因敲除方法为P1噬菌体转导敲除。

大肠杆菌利用噬菌体为媒介,将供体细胞DNA转移给受体细胞,从而使受体细胞的基因型和表现型发生改变,这一过程称为转导。

P1噬菌体的DNA分子量为5.8×107Da,在复制过程中,头部包装时容易发生错误包装,可将其宿主菌(供体菌)的基因组DNA误包入蛋白质衣壳内。

当用这一噬菌体裂解液侵染新的宿主菌(受体菌)时,供体菌的DNA片段可随P1噬菌体进入受体菌内,并可以与受体菌基因进行同源重组,从而永久性的存在于受体菌基因组上。

P1转导的频率一般很低,需要选择性标记进行转导子筛选,本实验中为卡那霉素抗性基因(KmR)。

准备材料LB液体试管,LB半固体培养基,LB固体培养基,20%(w/v)葡萄糖溶液,1M CaCl2溶液,1M MgSO4溶液,氯仿,1M柠檬酸三钠溶液;效价109-1010(pfu/mL) P1噬菌体储液,大肠杆菌溶源性供体菌,大肠杆菌受体菌;TM buffer:10mM Tris-HCl(pH7.4)buffer加入10mM MgSO4;P1盐溶液:10mM CaCl2与5mM MgSO4的混合溶液;实验步骤平板培养法准备野生菌种子液自保存甘油管或划线培养平板上,转接到3mL LB液体试管中,37℃,250rpm,培养过夜(12hr)。

制备野生型P1噬菌体1.将野生型菌株的过夜种子液,按1%(v/v)转接5mL LB液体试管,摇床培养至对数中期(~2*108细胞/mL,OD600=0.2~0.3)。

2.加入5mM CaCl2,继续培养至菌体细胞OD600=1。

3.取出野生菌培养液,低温聚菌,加入2倍体积的TM buffer重悬菌体。

4.倒下层平板,LB固体培养基中加入5mM CaCl2。

5.取2.5mL半固体LB培养基,加入0.25mL重悬菌液,混合均匀后倒在下层平板上,轻柔地倾斜平板使其均匀地覆盖在下层培养基表面。

T7RNA聚合酶催化的荧光扩增技术检测结直肠癌循环肿瘤细胞hTERT

T7RNA聚合酶催化的荧光扩增技术检测结直肠癌循环肿瘤细胞hTERT

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酵母双杂交表达载体pGBKT7_NPR1与pGADT7_NPR1的构建及酵母菌的转化

酵母双杂交表达载体pGBKT7_NPR1与pGADT7_NPR1的构建及酵母菌的转化
s t r e a m g e n e s .I n t h i s s t u d y .C DS s e q u e n c e o f 1 wa s c l o n e d i n t o t wo y e a s t e x p r e s s i o n v e c t o r s p GB KT 7 a n d p GA DT 7,
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迈瑞试剂日立上机参数

迈瑞试剂日立上机参数

校准方法 Calib Type Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Logit-Log(5p) Logit-Log(5p) Logit-Log(4p) Linear Linear
1浓度 0 0 0 0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0 0.00 0 0 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0
2浓度 # # # # # # # # # # # # # # # # # # # # #
# #
CK CKMB Ca Mg P α -AMY PA IgA IgG IgM C3 C4 CRP hs-CRP ADA
50 50
50 75 100 100 50 100 75 50 150 90 90 40 40 50 40 50 150 60 30 60 40
18800 11700 32000 32000 32000 32000 32000 32000 32000 32000 32000 32000 32000 32000 32000 32000 32000 0 32000 32000 32000 0 5000 32000 32000 32000 32000 32000 32000
2-2 2-2 2-2 2-2 2-2 2-2 6-3 6-3 6-3 6-3 6-3 6-3 6-3 5-3 2-2 2-2 2-2 6-3 2-2 2-2 2-2 2-2 2-2 2-2 6-3 2-2 2-2 2-2 2-2

实时荧光定量PCR仪ViiA7操作步骤

实时荧光定量PCR仪ViiA7操作步骤

实时荧光定量PCR仪ViiA操作步骤7——以 RNase P示例实验为例一、定义384孔样品模块的实验属性打开电脑访问ViiA 7 软件,然后打开左侧仪器开关。

单击Experiment Setup 图标。

单击Experiment Properties 以访问 Experiment Properties 屏幕。

在 ViiA 7 软件中设计 RNase P实验示例时,请输入:二、使用Define 屏幕定义RNase P 示例实验的目标基因、样品。

1.单击 Define 以访问 Define 屏幕。

2.定义目标基因a.单击 New 以增加和定义目标基因。

b.在目标基因表中,单击 Target Name 列中的一个单元格,并输入:c.(可选)单击 Save 以便将新增或原有的正在编辑的目标基因保存到Target Library 。

d.单击 Add Saved 从目标基因库添加目标基因。

3.定义样品a.单击 New 以增加和命名样品。

b.在样品表中,单击 Sample Name 列中的一个单元格,并输入:c. (可选)单击Save 以将新增或原有的正在编辑的样品保存到Sample Library 。

d.单击 Add Saved 从样品库添加样品。

4.(可选)定义生物学平行测定a. 在 Define Biological Replicates Groups 表中,单击 New 以增加和命名生物学平行测定组。

b.从下拉菜单选择 Color 。

c.单击 Comments 列,以便为该生物学平行测定组添加注释。

注:实验示例不使用生物学平行测定组。

保留Biological Replicate Groups 空白。

5.选择用作参比荧光的染料ROX 。

三、分配目标基因、样品和生物学组利用 Assign(分配)屏幕将目标基因、样品和生物学平行测定组分配到RNase P 实验示例反应板内的各孔。

注:您可以在不进行分配的情况下开始运行,但在扩增曲线中,将不会有实时数据(只有在您已经对板进行设置后,才可以见到扩增曲线)。

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RPN – Integrated Practice Test VII (A):1.What assessment should the PN be alarmed for possibility of toxicity due to cardiac glycosides?a.K⁺ level of 3.1 mEq/L – hypokalemia is a predisposing factor to Digoxin toxicity and NOT a signs ofDigoxin toxicityb.Hearing lossc.Yellow vision, poor appetite – these are signs of Digoxin toxicityd.Dry mucous membranesAnswer: C – these are signs of Digoxin toxicity2.Twenty four hours post laparoscopy, the nurse noted bleeding in dressing. Important nursing action:a.assess for abdominal distentionb.check patient vital signsc.position the client to semi-fowler‘s positionrm the physicianAnswer: B – vital signs (hypotension and tachycardia) are additional assessment that will confirm bleeding3. A 9-month old baby had repair of the foot and medication for pain was given as ordered. The mother said,―Why is the drug not working on my baby?‖ What is the right response?a.call the doctor for analgesicb.explain the meds will have an effect this timec.re-assess the child for further complicationsd.give another dose of pain analgesicAnswer: C – following pain medication, some pain relief is expected, however, other factors (post op complications) may contribute to discomfort.4. A child who sustained burn was treated successfully. On discharge teaching, the P N will teach the child‘smother that infection can be prevented by:a.Holding the baby with sterile glovese bleach when doing the laundryc.Changing the diaper as necessaryd.F requent hand washingAnswer: D –hand washing is the single and most effective way of preventing infection5.Top priority to promote safe nursing action to newborn following delivery?a.perform APGAR scoring at 1-5 minute after birthb.warm the infantc.establish respiratory rate and heart rated.place in supine positionAnswer: C –keeping the newborn‘s airway is the top priority following birth. Suctioning, side lying, and trendelenburg positions are different ways of keeping the airway patent.6.Which diet is appropriate to a client with stage 4 ulcer in the posterior chest?a.ice cream; milk; fruitskshake using skim milk; green vegetables; fruitsc.diet high in roughage; milkshake; fruitsd.c itrus fruits; green vegetables; white chicken; milkAnswer: D – vitamins and minerals (from fruits and vegetables) along with protein (milk and chicken) promote tissue repair and healing7. A student nurse, supervised by the PN, has performed the wound dressing on a client with abdominal wound.When documenting the procedure in the progress note, thea.Student nurse should documentb.The RN should documentc.The teacher should documentd.The attending physician should documentAnswer: A – the student has performed the wound care and wound dressing and thus she/he is responsible for the documentation8.Which of the following foods will promote tissue repair?a.Vegetablesb.Roughagec.Carbohydratesd.N utsAnswer: D – nuts contain protein and this is good for tissue repair9.Thrombus formation is a danger for all post operative patients. The nurse should act independently toprevent this complication by:a.Applying elastic stockingsb.Massaging gently with lotionc.Encouraging in-bed exercisesd.Providing adequate fluids intakeAnswer: C –inactivity causes venous stasis, hypercoagulability, and external pressure against the veins, all of which lead to thrombus formation.10. The PN recognizes that a pacemaker is indicated when a patient is experiencing:a.Anginab.Chest painc.Heart blockd.TachycardiaAnswer: C – This is the primary indication for pacemaker because there is an interference with the electrical conduction system of the heart.11. An essential nursing function in the care of a patient with arterial insufficiency in the left foot caused by generalized arteriosclerosis should be to:a.Maintain elevation of the legs.b.Massage the legs when painful.c.Check arterial pulse frequently.d.Apply a hot water bag to the feet.Answer: C – an altered quality of a variety of pulses in the extremity is the earliest indication of limited circulation.A – would interfere with gravity.B- this can release an embolus into the circulation; it may also cause tissue trauma.D – altered sensation may limit sensitivity to heat, which could result in burns.12. A client can‘t take deep breath and refuses to get out of bed because his chest tube is causing himdiscomfort. To promote the cli ent‘s compliance with ambulation and deep breathing, the PN should:a.Administer pain medication before having the client deep breathe, cough or get out of bedb.Tell the client the importance of lung expansionc.Arrange a care schedule to provide rest periodd.Teach the client how to use an incentive spirometerAnswer: A – ambulation and deep breathing are causing discomfort to the patient. Pre-medication will help the patient to perform these activities more effectively13. A client is recovering from an ileostomy performed to treat inflammatory bowel disease. During dischargeteaching, the PN should stress:a.Increasing fluid intake to prevent dehydrationb.Wearing an appliance pouch only at bedtimec.Consuming a low protein, high fiber dietd.Taking only enteric-coated medicationsAnswer: A – ileostomy continuously drain liquid or very soft stools. Adequate and extra fluids must be taken by the patient14. A client with increasingly difficulty swallowing, weight loss and fatigue had just received a diagnosis ofesophageal cancer. Because this client has difficulty in swallowing, the PN should give the highest priority to which action?a.Helping the client to cope with body image changesb.Ensuring adequate nutritionc.Maintaining patent airway – this is not necessary for the affected part is the esophagusd.Preventing injuryAnswer: B – difficulty swallowing may prevent the patient to eat necessary amount of food needed by the body. Soft or pureed diet or foods via NGT ensure nutrition15. A client with heart failure develops pink frothy sputum, coarse crackles and restlessness. Which of thefollowing actions should the PN take first?a.C heck the client‘s BPb.Place the client in high fowler’s positionc.C alculate the client‘s fluid balanced.Notify the physicianAnswer: B – these manifestations are indication of congestion in the lungs. High fowl er‘s position will provide relief from breathing problem. The physician must be notified after.16.The PN is observing a one day-old neonate. Which assessment finding indicates that oxygen needs are notbeing met by current treatments?a.RR 54 bpm – the normal RR is 30 to 60 bpmb.Abdominal breathing – newborns are abdominal breathersc.Nasal flaringd.Acrocyanosis – this is normally observed in most newborns due to the developing peripheral vascularsystemAnswer: C – nasal flaring is a sign of breathing problem in newborn17. A 15-month-old toddler is admitted with a diagnosis of croup and was placed in a mist tent with oxygen.The PN checks the child when he‘s asleep and notes the heart rate has increased to 120 b pm and RR to 36.What should the nurse do?a.Notify the MD immediatelyb.Notify the ICU for possible admissionc.Check the child’s SaO2 level using pulse oxymetryd.Check the child‘s BPAnswer: C - the normal heart rate for 15 month old ranges from 90 to 110 bpm; normal RR should be less than 40 bpm; the child is in the croup tent and sound asleep with HR of 120 and RR of 36.18. A 3-week-old infant was admitted with possible diagnosis of pyloric stenosis. When caring for the baby,which nursing intervention would be most important?a.Place the infant on his back for sleepb.Weigh the infant Q 12 hoursc.Position the infant in an infant seatd.Take the infant‘s VS q 8 hoursAnswer: A – supine position is the safest to all newborn babies, however, following feeding, upright position is preferred to prevent aspiration specially if the newborn vomits. Side lying or prone positions are highly associated with SIDS.19. A 3-year-old is brought to the ER with fractured wrist and suspicious looking bruising on his arms. Thestep father claims that the boy fell out of bed. What is the most important criterion for the PN to considerwhen deciding to report suspected child abuse?a.Inappropriate parenteral concern for the degree of injuryb.Absence of parents for questioning about the injuryc.Inappropriate response of the child to the injuryd.Incompatibility between the history and injuryAnswer: D – in child abuse, the historical account is greater than the actual injury20. A 28-year-old was admitted in the ER with multiple laceration and bruises to her face. The PN is aware thatthe client has a history of being abuse. What is the role of the PN in domestic abuse?a.Document the findings and provide support to the victimb.Protect the client privacy by not documenting the abuse – documenting the finding is a legal responsibilityc.Provide counseling for the abuse –this is not the RN‘s roled.Provide counseling to the victim –this is not the RN‘s roleAnswer: A – reporting abuse, documenting the finding, and providing support to the victims are the primary responsibilities of the PN21.The PN is caring for a client with wound infection who’s on strict isolation. When leaving the room of theclient, which protective equipment should the PN remove first?a.capb.maskc.gownd.glovesAnswer: D – wound will prov ide the RN’s direct exposure to the patient’s wound. Clean gloves will afford protection to the PN22.One of the most important aspects of pre-operative care of a neonate born with myelomeningocele ispositioning. Which of the following position is most important?a.supine and flatb.side-lying and flatc.prone with hips elevatedd.prone with head elevatedAnswer: C – prone position prevent pressure on the sac in the spine23.After performing a physical examination and obtaining a health history on a newly admitted client, the PNshould document her finding. When documenting the information in a client medical record, the PN should do which of the following?a.Erase any errore # 2 lead pencilc.Leave one line blank before each new entryd.End each entry with the PN’s sign ature and titleAnswer: D –the legal aspect of nurse‘s documentation and each entry made by the RN include date, signature, and title of the nurse.24. A PN is preparing a client with AIDS for discharge to home. Which instruction should the PN be sure toinclude?a.apply disposable gloves before showeringb.sterilize all plates and utensils in boiling waterc.avoid sharing toothbrushes and razorsd.avoid eating foods from serving dishes shared by other family membersAnswer: C – insignificant bleeding from minor cuts may results with the use of toothbrush and razor blades. The cuts in the skin will serve as port of entry for HIV virus25.Mrs. H was admitted with severe dehydration. She was placed in an isolation room with a foley cath inplace. The RN noted no ―urine output‖ in the foley bag. What pertinent assessment performed by the PN is important?a.Check the tube placementb.Palpate the bladder–the patient was severely dehydrated and perhaps there was no urine form yetc.Remove the catheter and reinsertrm the physician26.The patient was admitted with dehydration. Which question asked by the PN can be used as basis inassessing the patient‘s hydration status?a.When was the last you peed?–this is an assessment of fluid in the bodyb.When was the last time you drink?c.When was the last you ate?d.When was the last time you moved your bowel?27.Seven days following a successful recovery from TURP, which of the following statement made by the PNfocuses on preventing possible post op complication?a.―You will expect your urine to have small amount of blood‖b.―You must continue doing kegal exercise‖c.―You may now resume sexual intercourse‖d.“You need to avoid strenuous exercise”–this may precipitate bleeding28.While you were doing your late afternoon rounds, you‘ve noticed fire coming from the nurse‘s lounge.What will be the most appropriate initial action?a.Pull the fire alarm–remember the acronym RACE when dealing with fireb.Close all doors in the unitc.Try to put out the fired.Start evacuating the patient to a safer place29. A nurse manager from the medical unit asks one of the staff to continue working after a 12 hour shiftbecause one of the staff was not able to come to work. What would be the staff appropriate response?a.Accept the offer if the hospital compensates fairlyb.Stay and ensure for an overtime payc.Ask the manager to call for a relief and continue working until the relief arrives–the welfare of thepatient should be the top priority when working extra shift; if the nurse is already tires, anarrangement like this should be doned.Stay and manage the complexity of the workload by distributing the load to the staff30.Your patient handed you an enveloped with large sum of money. What would you do?a.Take it as an appreciation to your excellent nursing careb.Take the money and inform your colleaguec.Instruct the patient to donate the funds to the hospital–this is appropriated.Take the money and keep it in the unitCase Study:Three days ago, Mr. Arnold is a 78-year-old ♂ patient, was admitted in the community hospital due to exacerbation of his emphysema and chronic bronchitis. In preparation for his discharge, he was referred to the respiratory therapist (RT) and was taught deep breathing and coughing exercises to improve his oxygenation.Questions 79 to 83 refer to this case31.Based on the progress‘ note entry made by the RT, Mr. Arnold was not performing deep breathing andcoughing exercises taught to him. In this situation, the most appropriate action the PN takes would be:a.Assess and identify if the patient has some learning issues.b.Investigate if the patient‘s discharge can be delayed until he learns the procedure.c.Emphasize the importance of deep breathing and coughing exercises to the patient.d.Refer the patient to the respiratory therapist.Answer: A – the most common cause of the inability of the patient to perform the coughing and breathing exercises taught to him was lack of understanding of the importance of the teaching. The nurse needs to validate this aspect before taking further steps.32.How would the PN ensure that Mr. Arnold adheres to the coughing and breathing exercises post-discharge?a.Set up a follow-up appointment.b.Provide thorough health teachings before discharge.c.Refer the patient to the visiting nurse.d.Refer the patient to the home care management.Answer: A – setting up a follow up appointment enables the nurse to assess the patient and his compliance with the health teaching.33.While still in the hospital, Mr. Arnold fell on the floor from his chair. The PN rushed to rescue and foundthe patient very pale, cold, and clammy. Which actions made by the PN is most appropriate?a.Call the doctor; perform CPR.b.Loosen the patient‘s shirt‘s buttons and tie.c.Call for assistance.d.Ask the nursing aide to stay with the patient; while the nurse gets the code blue cart.Answer: C – if appropriate, the nurse may need an assistance to carry the patient back to the bed.Case StudyFatima is a 75-year-old-♀ patient, origina lly came from Iran, was admitted in the medical cardiac unit. Questions 84 to 85 refer to this case34.She complains of chest pain although it was described as mild, she was given NTG SL. After the 3rddose, she claims that she was still feeling the mild chest pain. Which of the following actions taken by the PN is appropriate?rm the RNb.Monitor VSc.Ensure strict bedrestd.Give another NTGAnswer: A – the physician must be informed since the patient has already received the maximum doses of NTG. Usually if chest pain persists after the 3rd dose of NGT, a more complicated and serious heart problem (like MI-myocardial infarction) has occurred.35.Fatima‘s serial cardiac enzymes tests confirmed an MI complicated by an acute heart failure or congestiveheart failure. Upon learning the outcome, Fatima became very depressed. The PN has taken the mostappropriate course of action by doing which of the following?a.Arrange that the patient be transferred to a private room.b.Arrange that the patient be transferred to th e room nearest to the nurse’s station.c.Place the patient in an orthopneic position.d.Ensure that the patient strictly follows her prescribed diet.Answer: B –the patient is depressed and very prone to suicide attempts. A room closer to the nurses‘ statio n will allow the nurses and other staff to constantly see and/or observe the patient.Case Studyboratory tests are performed on a patient with diabetic ketoacidosis. The PN should expect the tests toreveal:a.Low serum glucose, increased acidity, high carbon dioxide.b.Low serum glucose, decreased acidity, low carbon dioxide.c.Elevated serum glucose, normal acidity, high carbon dioxide.d.Elevated serum glucose, increased acidity, low carbon dioxide.Answer: D – diabetic ketoacidosis literally means high blood glucose; the pathophysiologic process includes the production of ketones (causing acidity) as the liver unconventionally uses ketones from fatty acids for energy; CO2 is low because this involves a metabolic process.37.The family of the elderly, aphasic patient, complains that the PN failed to obtain a signed consent beforeinserting an indwelling catheter to measure hourly output. This is an example of:a. A catheter inserted for the patient‘s benefit.b. A treatment that does not need a separate consent form.c.Treatment without consent of the patient, which is an invasion of rights.d.Inability to obtain consent for treatment because the patient was aphasic.Answer: B – on admission, patients or their proxy signs consent for general, non-invasive procedure that are commonly performed. These include starting peripheral IV; venipuncture; IM injection; inserting foley cath, ect.38.The patient has a bronchoscopy in ambulatory surgery. To prevent laryngeal edema, the PN should:a.Place ice chips in the patient‘s mouth.b.Offer the patient liberal amounts of fluid.c.Keep the patient in the semi-fowler’s position.d.Tell the patient to suck on medicated lozenges.Answer: C- with the head elevated, rather than horizontal or dependent, fluid will not collect in the interstitial spaces around the trachea.39.Christy is a 75-year-old elderly female patient who has a history of controlled diabetes mellitus andosteoarthritis of her right arm. She lives alone in her apartment and able to manage her activities of dailyliving without assistance. Three days ago she was admitted in the hospital for a total hip replacement of the L hip. She is now on her 2nd day post op. Nursing care plan promoting circulation for Christy would include:a.Isometric quadriceps exercise.b.Range of motion exercise.c.Active and passive range of motion exercise.d.Place abduction pillows in between the legs.Answer: A – this is the best and safest exercise that promotes circulation and prevents hip dislocation post hip replacement post hip replacement. Post hip replacement, the affected hip should remain extended and abducted. Isometric Exercise, no joint movement but has muscle contraction.40.The incidence of anemia among young adults is progressively increasing. Which of the followingpredisposing factors may cause anemia among young adults?a.sleeping patternb.not eating meat & vegetablesc.WBC 9000d.drinking too much alcoholic beveragesAnswer: B – meat and vegetables are rich in iron. Iron is essential in RBC production.41.James is a young-13-year-old teen-ager who presented himself to the school nurse due to his acne andlesions noticeable in his forehead and chin. The boy claims that he is seriously bothered by his physicalappearance and that he needs help to clear up the lesions. Based on this assertion, the nurse will suggestthat the young boya.avoids exposing himself, especially his face, to the sunb.dries his face with 100% cotton towel following a warm shower.es mild soap for his face and face wash that contains the lowest concentration of alcohol andhydrogen peroxide.d.limits or avoids sweets, chocolate, and fried foods.Answer: D – foods traditionally blamed for acne breakouts include nuts, chocolate, sweets, fried foods and milk. Thus, the best way to teat acne is by changing the diet.42.Mr. Chu was admitted in the hospital with a diagnosis of kidney infection. On physical assessment, hecomplains of tenderness in his flank area. He said that he has been eating poorly for about 3 days nowand he is also nauseated. In v iew of the nurses‘ findings, an appropriate intervention for the patientwould be:a. To d rink at least 3 to 4 L of fluid per day.b. To take sitz baths 3 to 4 times per day for urethral burning.c. To void immediately after sexual intercourse.d. To avoid exposure to persons with upper respiratory infections.Answer: A - kidney infection occurs when bacteria gets access to the bladder. Large amount of fluids promote urination, empty and wash out bacteria in the bladder.43.Michael is a 33-year-old openly self- pronounced homosexual, was admitted for a pulmonary infection. Heis positive for HIV and an active user of illicit drug. He was treated with combinations of antibiotics. His recovery was uneventful and today he is being discharged to home. Which of the following patient‘sstatements indicate a thorough understanding of the discharge instructions?a.―Condom will not be required for u s during sex as my partner and myself are both HIV⁺.‖b.―I will start donating blood again as soon I‘m fully recovered.‖c.―I will keep a box of condom in my glove compartment and make them readily available.‖d.“I’ll see to it that a sharp container for my used needles is placed in a secured place at home.”Answer: D – sharp container for used needles promote safety not only for the patient but also for other people living with the patient.44.In evaluating the client‘s fluid balance, which of the following findings would most likely require anintervention:a.Intake is greater than outputb.Output is greater than intakec.Weight lossd.Weight gainAnswer: A – output is essentially desired to be greater than intake. Intake more than output can stress the heart especially in elderly patients and/or patients with heart and kidney problems45.Pritzy, a 21-year-old young lady, was raped by her boyfriend. Following an extensive investigation in theemergency department, Pritzy decided to withdraw charges against her boyfriend. The PN who is involved in the care of the patient will appropriately do which of the following?a.The PN states, ―It‘s really upsetting when people have different opinions.‖b.The PN states, “The client’s decision must be respected”.c.The PN agrees with the medical team that the patient should press charges against her boyfriend.d.The medical team should perform a more extensive investigation.Answer: B –the patient‘s decision must be respected even if it is against the health team‘s view.46.Candice is a young girl who was admitted in the ER due to an acute asthma attack. She was placed inthe ER holding area and waiting for a bed in the medical unit. She receives bronchodilating agents(Albuterol) via hand held nebulizer and was started on IV Solucortef. Which of the following changes in the patient‘s condition best indicates th at Candice is responding favorably to the medications?a.RR 22; wheezing decreased.b.HR 90; BP 100/64.c.Urine output 40 ml last 2 hours; BP 100/64.d.RR 18; HR 54.Answer: A – normal respiratory rate and decreased wheezing are signs of bronchodilation, a desired effect of the albuterol.47.Nursing assessment indicating effective fluid resuscitation on a patient who sustained 2nd and 3rd degreeburnsinclude:a.Urine output of 100 ml for the last 2 hours.b.Skin recoils when pinch.c.Hematocrit level higher than the previous reading.d.Increasing heart rate.Answer: A – increased urine output is a good indication that the fluids replaced in a burn patient was successful. This means that the kidneys are adequately perfused.rry is a 38 year-old male patient, HIV+ and has history of Hepatitis C, presents with 4-day history of earproblem. He also complains of dizziness, headache, and loss of balance when ambulating. He states, ‗I feel bugs crawling to my left ear, I need a flush‘. He has seen 4 different doctors about his ear problem but‗nothing was ever done‘ he claimed. Following otoscopic examination, the physician ordered earirrigation. The PN who performs the procedure can prevent transmission of HIV infection by which of thefollowing precautionary measures?a.Wear gloves when irrigating the patient’s ear.b.Wear mask and gown when irrigating the patient‘s ear.c.Isolate the patient during the ear irrigation procedure.d.Wash hands before and after the procedure.Answer: A – HIV virus can be transmitted through body fluids. Used of clean gloves when irrigating the ear of the patient who is HIV⁺ prevents the nurse from being directly exposed to the body fluids of the patient. This is usually sufficient protection.49.Viktoriya, a 74-year-old Russian immigrant, was being discharge to home following blood sugar controlfor her diabetes. She was diagnosed with type 1 DM when she was 8 years old. She sustainedpoor/impaired vision from her DM; had a mild stroke 5 years ago; heart failure; and well-controlledhypertension. She was prescribed different medications for maintenance of her blood pressure, heartproblem and other medical condition. An important discharge teaching for this patient who will be takingdifferent medications that come in tablets forms and different colors would be:a.Encourage the technique of color flagging.b.Encourage the patient to use magnifying glass.c.Instruct the patient to store the pills in different places in the house.cate the patient on the sizes and shapes of the pills.Answer: D – a patient who is visually impaired usually has a well-developed sense of touch (as well as other senses) to compensate for loss of good vision.50.Understanding that there is a need to protect susceptible persons from exposure to chickenpox during theacute phase, the PN should question the mother of a child with chickenpox about relatives or friends whoare receiving:a.Long-term anticonvulsant therapy.b.Prolonged topical antibiotic therapy.c.High doses of systemic steroid therapy.d.Therapeutic doses of vitamins and minerals.Answer: C – individuals taking steroids have lowered resistance and may become fatally ill if exposed to varicella virus.51.The patient weighed 210 pounds on admission to the hospital. After 2 days of diuretic therapy the patientweighs 205.5 pounds. The PN could estimate that the amount of fluid the patients has lost is:a.0.5 Lb. 1.0 Lc. 2.0 Ld. 3.5 LAnswer: C – one liter weighs approximately 2.2 pounds; therefore, a 4.5 pound weight loss equal approx 2 liters.52.When taking the BP of a patient who has AIDS the PN must:a.Wear clean gloves.e barrier techniques.c.Wear a mask and gown.d.Wash the hands thoroughly.Answer: D – blood and body fluid precautions require that hands be washed before and after patient care to minimize the risk of transmission.Case Study。

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