犬钩虫(Ancylostoma caninum)天冬氨酸蛋白酶基因的克隆和在大肠杆菌中的表达

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重庆荣昌犬钩虫ITS及5.8S rDNA的克隆及序列分析

录间隔区，包括ＩＴＳｌ和ＩＴＳ一２两段序列．由于ＩＴＳ区（内转录间隔区）在生物进化过程中显示了种的特征，
种内具有高度保守性，不同种间又有不同程度的变异，因此受到了普遍关注．许多研究表明，ＩＴＳ序列在寄生虫进化机制和系统重建研究上是一种有效的分子标记［７．本研究通过对犬钩虫ｒＤＮＡ的ＩＴＳ进行克隆、测序及序列分析，旨在为以后犬钩虫病的分子流行病学调查以及对于本病的防治进行更深入的研究收稿日期：２Ｏ１
３１１７２３１３）；西南大学博士基金资助项目（１１ＢＳｒ０４）基金项目：国家自然科学基金资助项目（作者简介：黄
丽（１９９１一），女，重庆荣昌人，本科生，主要从事畜禽寄生虫病学研究．
文献标志码：Ａ
中图分类号：Ｑ９６８．９
犬钩虫病是由钩口科钩口属的犬钩虫Ａｎｃｙｌｏｓｔｏｍａｃａｎｉｎｕｍ引起的一类线虫病，主要寄生于犬的小
肠，尤其是十二指肠，以犬贫血、胃肠功能紊乱以及营养不良为主要特征＿１］．国内董文阳等在南京流浪犬体内亦发现有此虫，其感染率达５４．９１，平均感染强度（ＥＰＧ）为２．６３ ×１０。；张玲等对洛阳市９０只患肠道疾病的宠物犬进行钩虫感染情况调查，发现犬钩虫感染率为１８．８９．澳大利亚曾报道犬钩虫感染率

华支睾吸虫组织蛋白酶D样天冬氨酸蛋白酶基因的克隆表达和重组蛋白的免疫学分析

进一步研究该蛋白的功能奠定隆；织蛋白酶Ｄ；冬氨酸蛋白酶；疫原性华基组天免
中图分类号：３３２文献标识码：Ｒ８．Ａ
ＣｌｎｎｇａｒｋｒｏｉｘｅｓｏｆｃｔｅｓｎＤｉｓｒｉｏｅｓｅｅｏｉｎｄｐｏａｙｔｃｅｐｒｓｉｎｏａｈｐｉｌｋｅａｐａｔｃｐｒｔａｅｇｎｏｏｏｃｉｉｅｓｓａｄａａｙｉｆｉｕｏｅｉｉｙｏｈｅｏｉａｔｐｏｅｎｆＣｌｎｒｈｓｓｎｎｉｎｎｌｓｓｏｍｍｎｇｎｃｔｆｔｅｒｃｍｂｎｎｒｔｉ
维普资讯
２００８，２（）４１
中国人兽共患病学报
ＣｈｉｓｏｕｎａｆＺｏｏｓｓｎｅｅＪｒｌｏｏｎｅ５
文章编号：０２６４２０）１０５４１０ —２９（０８０ —００ —０
ＺＨＡＯｕ — ｏｇ，ＪｎｈｎＨＵ — ｈ，ＸｕｃｕＸＵｉＨＵｎ－ｕ，Ｊｎ，ＦｅｇｙＺＨＯＵｎ－ｕｎ，ＨｏｇｊａＨＵｉｉ，Ｈｕ— ａｘ
ＺＨＥＮＧａ —ｉＸｉｏｌｎｇ，ｎ— ｎ，ＬＩＹａｗｅＹＵｎｂｉＸｉ — ｎ（ｐｒｍｅｔｆＰａａｉｏｏｙ．ｈｎｓａｄｉａｃｏｌｕｔｓｎＵｎｖｒｉｙ，ＤｅａｔｎｒｓｔｌｇＺｏｇｈｎＭｅｃｌＳｈｏ，ＳｎＹａ—ｅｉｅｓｔｏＫｅｂｒｔｒｏｏｉａｓａｅＣｎｒｌａｄＰｒｖｎｉｎｏｈｎｓｒｆＥｄｕａｉｎ，Ｃｕｎｈｕ５０８ｙＬａｏａｏｙｆｒＴｒｐｃｌＤｉｅｓｏｔｏｎｅｔｏｆｔｅＭｉｉｔｙｏｅｃｔｏｒａｇｚｏ００，Ｃｉａ１ｈｎ）

天冬氨酸蛋白酶在寄生虫感染与免疫中的重要作用

Open Journal of Nature Science 自然科学, 2019, 7(2), 41-44Published Online March 2019 in Hans. /journal/ojnshttps:///10.12677/ojns.2019.72007The Important Role of Aspartate Protease in Parasitic Infection and ImmunityYuexin Sun1, Shaojie Wang1, Yuning Mao1, Yingying Bai1, Xiaojing Sun21Department of Medical Technology, Xi’an Medical University, Xi’an Shaanxi2Department of Pathogenic Biology, School of Basic Medicine Science, Xi’an Medical University, Xi’an ShaanxiReceived: Jan. 30th, 2019; accepted: Feb. 11th, 2019; published: Feb. 18th, 2019AbstractAspartate protease is a kind of important alkaline proteolytic enzymes, which widely exists in a variety of animals and plants, and participates in the metabolism and immune regulation of the body. In this paper, we briefly discussed the structure and function of aspartate protease and its important role in parasitic infection and immunity, so as to lay a foundation for the study of its potential therapeutic targets and candidate vaccines.KeywordsAspartate Protease, Parasite, Immunity天冬氨酸蛋白酶在寄生虫感染与免疫中的重要作用孙悦馨1，王少杰1，毛玉宁1，白萤萤1，孙晓敬21西安医学院医学技术系，陕西西安2西安医学院基础医学部，病原生物学教研室，陕西西安收稿日期：2019年1月30日；录用日期：2019年2月11日；发布日期：2019年2月18日摘要天冬氨酸蛋白酶是一类重要的碱性蛋白水解酶，广泛存在于多种动植物体内，参与机体的新陈代谢和免疫调节。

whonet5[1].4中英文菌名及其代码

1 ORG ORGANISM2 saj Abiotrophia (Strep.) adiacens/毗邻乏氧菌3 sdf Abiotrophia (Strep.) defectiva/缺陷乏氧菌4 abi Abiotrophia sp./乏氧菌属5 acf Absidia corimbifera/伞枝犁头霉6 abs Absidia sp./犁头霉属7 acn Acanthamoeba sp./棘阿米巴属8 avb Acetivibrio sp./醋弧菌属9 ace Acetobacterium sp./醋杆菌属10 acp Acholeplasma sp./无胆甾原体属11 ach Achromobacter sp./产碱菌属12 ade Achromobacter (Alc.) xylosoxidans ss. denitrifican13 axy Achromobacter (Alc.) xylosoxidans ss. xylosoxidans14 afb Acid fast bacilli/抗酸杆菌15 afe Acidaminococcus fermentans/发酵氨基酸球菌16 acd Acidaminococcus sp./氨基酸球菌属17 adf Acidovorax delafieldii/德氏食酸菌18 afc Acidovorax facilis/敏捷食酸菌19 acx Acidovorax sp/假单胞菌属20 atm Acidovorax temperans/温和假单胞菌21 aan Acinetobacter anitratus/无硝不动杆菌22 aba Acinetobacter baumanii/鲍曼不动杆菌23 aca Acinetobacter calcoaceticus/醋酸钙不动杆菌24 aha Acinetobacter haemolyticus/溶血不动杆菌25 ajo Acinetobacter johnsonii/约氏不动杆菌26 aju Acinetobacter junii/琼氏不动杆菌27 alw Acinetobacter lwoffii/洛菲不动杆菌28 ac- Acinetobacter sp./不动杆菌属29 acr Acremonium (Cephalo.) sp./支顶孢属30 aff Acremonium falciforme/镰状支顶孢菌31 aki Acremonium kiliense/基利支顶孢菌32 arf Acremonium recifei/瑞塞菲支顶孢菌33 apl Actinobacillus (Haem.) pleuropneumoniae/大叶性肺炎放线杆菌34 aur Actinobacillus ureae/脲放线杆菌35 hac Actinobacillus actinomy/伴放线放线杆菌36 aeq Actinobacillus equuli/马驹放线杆菌37 ali Actinobacillus lignieresii/李氏放线杆菌38 acb Actinobacillus sp./放线杆菌属39 asu Actinobacillus suis/猪放线杆菌40 amu Actinomadura madurae/足肿马杜拉放线菌41 acm Actinomadura sp./马杜拉放线菌属42 apy Actinomyces (Coryn.) pyogenes/化脓隐秘杆菌43 abe Actinomyces bernardiae/伯尔德隐秘杆菌44 abo Actinomyces bovis/牛型隐秘杆菌45 agg Actinomyces georgiae/乔氏隐秘杆菌46 age Actinomyces gerencseriae/戈氏隐秘杆菌47 ais Actinomyces israelii/衣氏隐秘杆菌48 ame Actinomyces meyeri/麦尔隐秘杆菌49 ane Actinomyces naeslundii/内氏隐秘杆菌50 aat Actinomyces neuii ss. Anitratus/纽氏隐秘杆菌无硝亚种51 anu Actinomyces neuii ss. neuii/纽氏隐秘杆菌纽氏亚种52 aod Actinomyces odontolyticus/龉齿隐秘杆菌53 apk Actinomyces pyogenes-like bacteria/化脓隐秘杆菌54 act Actinomyces sp./隐秘杆菌属55 avs Actinomyces viscosus/粘性隐秘杆菌56 aec Aerococcus sp./气球菌属57 aun Aerococcus urinae/脲气球菌58 avi Aerococcus viridans/浅绿气球菌59 acv Aeromonas caviae/豚鼠气单胞菌60 aeu Aeromonas eucrenophila/嗜矿泉气单胞菌61 aeh Aeromonas hydrophila/嗜水气单胞菌62 aja Aeromonas jandaei/简达气单胞菌63 amd Aeromonas media/中间气单胞菌64 apn Aeromonas punctata/斑点气单胞菌65 apn Aeromonas punctata ss. punctata/斑点气单胞菌斑点亚种66 asa Aeromonas salmonic ida/杀鲑气单胞菌67 ash Aeromonas schubertii/舒氏气单胞菌68 aso Aeromonas sobria/温和气单胞菌69 aer Aeromonas sp./气单胞菌属70 atr Aeromonas trota/脆弱气单胞菌71 ave Aeromonas veronii/威隆气单胞菌72 asb Aeromonas veronii biovar sobria/维罗纳气单胞菌温和生物变种73 avv Aeromonas veronii biovar veronii/维罗纳气单胞菌维氏生物变种74 afo Afipia broomeae/布氏阿菲波菌75 afd Afipia clevelandensis/克利夫兰阿菲波菌76 apf Afipia felis/猫阿菲波菌77 afi Afipia sp./阿菲波菌属78 atu Agrobacterium radiobacter/放射土壤杆菌79 agr Agrobacterium sp./土壤杆菌属80 atu Agrobacterium tumefaciens (radiobacter) (CDC Vd-3)81 ajc Ajellomyces capsulatus/荚膜阿耶罗菌82 aje Ajellomyces sp./阿耶罗菌属83 afa Alcaligenes faecalis (odorans) (CDC VI)/粪产碱杆菌84 afa Alcaligenes odorans/粪产碱杆菌85 api Alcaligenes piechaudii/皮氏产碱杆菌86 alc Alcaligenes sp./产碱杆菌属87 axy Alcaligenes xylosoxidans/木糖产碱杆菌88 ade Alcaligenes xylosoxidans ss.denitrificans(Vc)/ 木糖氧化产碱菌反硝化亚种89 axy Alcaligenes xylosoxidans ss.xylosoxidans(Ⅲa,b)/ 木糖氧化产碱菌木糖氧化亚种90 aoi Alloiococcus otitidis/耳炎差异球菌91 alo Alloiococcus sp./差异球菌属92 aal Alternaria alternata/链格孢93 alt Alternaria sp./铰链孢属94 shn Alteromonas hanedai/羽田交替单胞菌（羽田希瓦菌)95 alm Alteromonas sp./交替单胞菌属96 aly Alysiella sp./小链菌属97 anc Anaerobic gram negative cocci/厌氧革兰阴性球菌98 anb Anaerobic gram negative coccobacilli/厌氧革兰阴性99 an- Anaerobic gram negative organisms/厌氧革兰阴性菌100 anr Anaerobic gram negative rods/厌氧革兰阴性杆菌101 apc Anaerobic gram positive cocci/厌氧革兰阳性球菌102 apb Anaerobic gram positive coccobacilli/厌氧革兰阳性103 an+ Anaerobic gram positive organisms/厌氧革兰阳性菌104 apr Anaerobic gram positive rods/厌氧革兰阳性杆菌105 avc Anaerobic gram variable cocci/厌氧革兰变异球菌106 avr Anaerobic gram variable rods/厌氧革兰变异杆菌107 agb Anaerobic gram variable coccobacilli/厌氧革兰变异球杆菌108 ana Anaerobic organisms/厌氧菌109 ans Anaerobic streptococci/厌氧链球菌110 anp Anaerobiospirillum sp./厌氧螺菌属111 asn Anaerobiospirillum succiniciproducens/产琥珀酸厌氧螺菌112 afr Anaerorhabdus (Bacter.) furcosus/叉形棍状厌氧菌113 arh Anaerorhabdus sp./棍状厌氧菌属114 anv Anaerovibrio sp./厌氧弧菌属115 abr Ancylostoma braziliensis116 acu Ancylostoma caninum/犬钩虫117 adu Ancylostoma duodenale/十二指肠钩虫118 any Ancylostoma sp./钩虫属119 acs Angiostrongylus cantonensis/广州管圆线虫120 aco Angiostrongylus costaricensis/脊形管圆线虫121 ang Angiostrongylus sp./管圆线虫属122 ank Anisakis sp./异尖属123 afn Aphanoascus fulvescens/黄褐隐囊菌124 aph Aphanoascus sp./隐囊菌属125 ppr Arachnia propionica/丙酸蛛网菌126 ahe Arcanobacterium (Coryn.) haemolyticum/溶血隐秘杆菌127 apy Arcanobacterium (Coryn., Actino.) pyogenes/化脓隐秘杆菌128 arb Arcanobacterium sp./隐秘杆菌属129 abu Arcobacter butzleri/布氏弓形菌130 acy Arcobacter cryaerophilus/嗜低温弓形菌131 ant Arcobacter nitrofigilis/硝化弓形菌132 arc Arcobacter sp./弓形菌属133 ari Arizona sp./亚利桑那菌属134 abl Arthroderma borellii/皮肤分节真菌135 acj Arthroderma cajetani/凯耶节皮菌136 ack Arthroderma cookiellum/库克节皮菌137 acc Arthroderma corniculatum/角突节皮菌138 afm Arthroderma fulvum/粉节皮菌139 agu Arthroderma grubyi/格鲁比节皮菌140 agy Arthroderma gypseum/石膏样节皮菌141 ain Arthroderma incurvatum/内弯节皮菌142 aob Arthroderma obtusum/钝节皮菌143 aot Arthroderma otae/太田节皮菌144 ape Arthroderma persicolor/桃色节皮菌145 ara Arthroderma racemosum/总状节皮菌146 ard Arthroderma sp./节皮菌属147 aka Arthrographis kalrae148 art Arthrographis sp./节图霉属149 alu Ascaris lumbricoides/似蚓蛔线虫(人蛔虫)150 asc Ascaris sp./蛔虫属151 acl Aspergillus clavatus/棒曲霉152 afs Aspergillus fischerianus/灰绿曲霉153 afv Aspergillus flavipes/黄柄曲霉154 afl Aspergillus flavus/黄曲霉菌155 afu Aspergillus fumigatus/烟曲霉156 afp Aspergillus fumigatus var. phialiseptus157 agl Aspergillus glaucus/灰绿曲霉158 agl Aspergillus glaucus group/灰绿曲霉群159 and Aspergillus nidulans/构巢曲霉160 ani Aspergillus niger/黑色曲菌161 asp Aspergillus sp./曲菌属162 asy Aspergillus sydowii/聚多曲霉163 ate Aspergillus terreus/土曲霉164 avl Aspergillus versicolor/杂色曲霉165 lmi Atopobium (Lacto.) minutum/小陌生物菌166 apv Atopobium (Strep.) parvulum/极小陌生物菌167 arm Atopobium rimae/龈裂陌生物菌168 ato Atopobium sp./陌生物菌属169 aub Aureobacterium sp./金杆菌属170 apu Aureobasidium pullulans/出芽短梗霉菌171 are Aureobasidium sp./短梗霉属172 aus Aureobasidium sp./短梗霉属173 bbs Babesia sp./巴倍虫属174 bal Bacillus alvei/蜂窝芽胞杆菌175 ban Bacillus anthracis/炭疽芽孢杆菌176 bbe Bacillus brevis/短芽胞杆菌177 bce Bacillus cereus/蜡样芽胞杆菌178 bci Bacillus circulans/环状芽胞杆菌179 bcg Bacillus coagulans/凝固芽胞杆菌180 bfi Bacillus firmus/坚强芽孢杆菌181 blt Bacillus laterosporus/侧胞芽胞杆菌182 bln Bacillus lentus/迟缓芽孢杆菌183 bli Bacillus licheniformis/地衣芽胞杆菌184 bmc Bacillus macerans/浸麻芽胞杆菌185 bmg Bacillus megaterium/巨大芽胞杆菌186 bmy Bacillus mycoides/蕈状芽胞杆菌187 bpn Bacillus pantothenticus/泛酸芽胞杆菌188 bpo Bacillus polymyxa/多粘芽胞杆菌189 bpm Bacillus pumilus/短小芽胞杆菌190 bcs Bacillus sp./芽胞杆菌属191 bsh Bacillus sphaericus/球形芽胞杆菌192 bsr Bacillus stearothermophilus/嗜热芽胞杆菌193 bsl Bacillus subtilis/枯草芽胞杆菌194 btu Bacillus thuringiensis/苏云金芽胞杆菌195 paa Bacteroides asaccharolyticus/不解糖拟杆菌196 bbi Bacteroides bivius/双向拟杆菌（二路普雷沃菌）197 pbu Bacteroides buccae/颊拟杆菌(颊普雷沃菌)198 pbc Bacteroides buccalis/口腔拟杆菌(口腔普雷沃菌)199 bcc Bacteroides caccae/粪拟杆菌200 bcp Bacteroides capillosus/多毛拟杆菌201 bco Bacteroides coagulans/凝固拟杆菌202 pco Bacteroides corporis/人体拟杆菌(人体普雷沃菌)203 bde Bacteroides denticola/牙拟杆菌(牙普雷沃菌)204 bdi Bacteroides disiens/解糖胨拟杆菌(解糖胨普雷沃菌) 205 bds Bacteroides distasonis/吉氏拟杆菌206 beg Bacteroides eggerthii/埃氏拟杆菌207 peo Bacteroides endodontalis/牙龈拟杆菌(牙龈卟啉单胞菌) 208 bfo Bacteroides forsythus/福赛斯拟杆菌209 bfr Bacteroides fragilis/脆弱拟杆菌210 bfg Bacteroides fragilis group/脆弱拟杆菌群211 afr Bacteroides furcosus/叉状假杆菌212 pgi Bacteroides gingivalis/龈拟杆菌213 bgr Bacteroides gracilis/纤细弯曲杆菌214 pin Bacteroides intermedius/中间型拟杆菌215 ble Bacteroides levii/里夫拟杆菌216 plo Bacteroides loescheii/罗斯拟杆菌217 bme Bacteroides melaninogenicus/产黑色素拟杆菌218 bmr Bacteroides merdae/屎拟杆菌219 boc Bacteroides ochraceus/黄褐二氧化碳嗜纤维菌220 pol Bacteroides oralis/口拟杆菌221 poi Bacteroides oris/口腔拟杆菌222 bov Bacteroides ovatus/卵圆拟杆菌223 dpn Bacteroides pneumosintes/侵肺拟杆菌224 bpu Bacteroides putredinis/腐败拟杆菌225 pru Bacteroides ruminicola/栖留胃拟杆菌226 prb Bacteroides ruminicola ss. Brevis/栖瘤胃普雷沃菌短亚种227 pru Bacteroides ruminicola ss. Ruminicola/栖瘤胃普雷沃菌栖瘤胃亚种228 bac Bacteroides sp./拟杆菌属229 bsp Bacteroides splanchnicus/内脏拟杆菌230 bst Bacteroides stercoris/便拟杆菌231 bte Bacteroides tectum/隐蔽拟杆菌232 bth Bacteroides thetaiotaomicron/多形拟杆菌233 bun Bacteroides uniformis/单形拟杆菌234 bur Bacteroides ureolyticus/解脲拟杆菌235 bvu Bacteroides vulgatus/普通拟杆菌236 bcl Balantidium coli/结肠小袋纤毛虫237 bat Balantidium sp./小袋纤毛虫属238 bba Bartonella bacilliformis/杆状巴尔通体239 bez Bartonella elizabethae/伊丽沙白巴尔通体240 bhe Bartonella henselae/横塞巴尔通体241 bqu Bartonella quintana/五日热巴尔通体242 bar Bartonella sp./巴尔通体属243 bvi Bartonella vinsonii/文森巴尔通体244 bra Basidiobolus ranarum/蛙粪霉245 bas Basidiobolus sp./蛙粪霉菌246 bea Beauveria sp./白僵菌属247 wzo Bergeyella (Weeksella) zoohelcum (CDC IIj)/ 动物溃疡伯杰菌248 ber Bergeyella sp./伯杰菌属249 bad Bifidobacterium adolescentis (CDC E)/青春双歧杆菌250 bbf Bifidobacterium bifidum/双歧双歧杆菌251 bbv Bifidobacterium breve/短双歧杆菌252 bct Bifidobacterium catenulatum/链状双歧杆菌253 bcr Bifidobacterium coryneforme/棒状双歧杆菌254 bdn Bifidobacterium dentium/齿双歧杆菌255 bin Bifidobacterium infantis/婴儿双歧杆菌256 blo Bifidobacterium longum/长双歧杆菌257 bmn Bifidobacterium magnum/大双歧杆菌258 bps Bifidobacterium pseudolongum/假长双歧杆菌假长亚种259 bif Bifidobacterium sp./双歧杆菌属260 bil Bilophila sp./嗜胆菌属261 bwa Bilophila wadsworthia/沃氏嗜胆菌262 bau Bipolaris australiensis/澳大利亚双极霉263 bha Bipolaris hawaiiensis264 bip Bipolaris sp./双极霉属265 bsi Bipolaris spicifera/长穗双极菌266 bho Blastocystis hominis/人酵母菌267 bls Blastocystis sp./酵母菌属268 bdr Blastomyces dermatitidis/皮炎芽生菌269 bla Blastomyces sp./芽生菌270 gca Blastoschizomyces (Geo.,Trich.) capitatus271 bsz Blastoschizomyces sp./芽生裂殖菌属272 bav Bordetella avium/鸟鲍特菌273 bbr Bordetella bronchiseptica (CDC IV a)/支气管炎鲍特菌274 bhz Bordetella hinzii275 bhm Bordetella holmesii (CDC NO-2)/霍氏鲍特菌276 bpa Bordetella parapertussis (CDC IVb)/副百日咳鲍特菌277 bpe Bordetella pertussis/百日咳鲍特菌278 bd- Bordetella sp./鲍特菌属279 e26 Bordetella-like species (CDC EF-26)280 baf Borrelia afzelii/阿氏疏螺旋体281 bbu Borrelia burgdorferi/布氏疏螺旋体282 bdu Borrelia duttonii/中非回归热螺旋体283 bga Borrelia garinii/嘎氏疏螺旋体284 bhr Borrelia hermsii/西北美回归热螺旋体285 bpk Borrelia parkeri/扁虱疏螺旋体（包柔氏螺旋体）286 brc Borrelia recurrentis/回归热螺旋体287 bol Borrelia sp./疏螺旋体属288 btr Borrelia turicatae/特里蜱疏螺旋体289 baa Brachyspira aalborgii290 brs Brachyspira sp./短螺菌属291 bca Branhamella catarrhalis/粘膜炎布兰汉菌(卡他莫拉菌) 292 mo- Branhamella sp./布兰汉菌属(莫拉菌属)293 bay Brevibacterium acetylicum/乙酰短杆菌294 bvc Brevibacterium casei/乳酪短杆菌295 bep Brevibacterium epidermidis/表皮短杆菌296 bmb Brevibacterium mcbrellneri/迈克布瑞德短杆菌297 bre Brevibacterium sp. (CDC B1,B3)/短杆菌属298 pdi Brevundimonas (Pseudo.) diminuta (CDC Ia)/ 缺陷短波单胞菌299 pve Brevundimonas (Pseudo.) vesicularis/泡囊短波单胞菌300 brv Brevundimonas sp./短波单胞菌属301 bro Brochothrix sp./索丝菌属302 bab Brucella abortus (melitensis)/流产（牛）布鲁菌303 bcn Brucella canis (melitensis)/狗布鲁菌304 bmt Brucella melitensis/马耳他（羊）布鲁菌305 br- Brucella sp./布鲁菌属306 bsu Brucella suis (melitensis)/猪布鲁菌307 bml Brugia malayi/马来丝虫308 brg Brugia sp./布鲁[丝虫]属309 bti Brugia timori/帝汶布鲁丝虫310 baq Budvicia aquatica/水生布戴约维采菌311 bud Budvicia sp./布戴约维采菌属312 pce Burkholderia (Pseudo.) cepacia (CDC EO-1, gen. I)313 bgl Burkholderia (Pseudo.) gladioli/唐菖蒲伯克霍尔德菌314 bma Burkholderia (Pseudo.) mallei/鼻疽伯克霍尔德菌315 ppi Burkholderia (Pseudo.) pickettii (CDC V a-1,2,3)/ 皮氏伯克霍尔德菌316 ppm Burkholderia (Pseudo.) pseudomallei/假鼻疽伯克霍尔德菌317 bam Burkholderia ambifaria (genomovar VII)318 bah Burkholderia anthina (genomovar VIII)319 bnc Burkholderia cenocepacia (genomovar III)320 bcx Burkholderia cepacia complex321 bdo Burkholderia dolosa (genomovar VI)322 bmu Burkholderia multivorans (genomovar II)323 bpy Burkholderia pyrrocinia (genomovar IX)324 buk Burkholderia sp./伯克霍尔德菌属325 btb Burkholderia stabilis (genomovar IV)326 bub Burkholderia ubonensis (genomovar X)327 bvt Burkholderia vietnamiensis (genomovar V)328 bua Buttiauxella sp./布丘菌属329 but Butyrivibrio sp./丁酸弧菌属330 cgr Calymmatobacterium granulomatis/肉芽肿鞘杆菌331 cmb Calymmatobacterium sp./鞘杆菌属332 bgr Campylobacter (Bact.) gracilis/纤细弯曲杆菌333 ccu Campylobacter (Wol.) curvus/曲型弯曲杆菌334 cre Campylobacter (Wol.) rectus/直肠弯曲杆菌335 cco Campylobacter coli/大肠弯曲杆菌336 ccn Campylobacter concisus (CDC EF-22)/简明弯曲杆菌337 hfn Campylobacter fennelliae/芬纳尔弯曲菌（螺杆菌）338 cfe Campylobacter fetus/胚胎弯曲杆菌339 cfe Campylobacter fetus ss. Fetus/胚胎弯曲杆菌胎儿亚种340 cfv Campylobacter fetus ss. V enerealis/胚胎弯曲杆菌性341 chy Campylobacter hyointestinalis/豚肠弯曲杆菌342 caj Campylobacter jejuni/空肠弯曲菌343 cj1 Campylobacter jejuni biotype 1/空肠弯曲杆菌生物I型344 cj2 Campylobacter jejuni biotype 2/空肠弯曲杆菌生物II345 cjd Campylobacter jejuni ss. Doylei/空肠弯曲杆菌德莱亚346 caj Campylobacter jejuni ss. Jejuni/空肠弯曲杆菌空肠亚347 clr Campylobacter lari/红嘴鸥弯曲杆菌348 clr Campylobacter laridis/海鸟弯曲杆菌349 cmu Campylobacter mucosalis/粘膜弯曲杆菌350 csh Campylobacter showae/昭和弯曲杆菌351 cam Campylobacter sp.弯曲杆菌属352 csp Campylobacter sputorum/唾液弯曲杆菌353 cbb Campylobacter sputorum ss. Bulbulus/唾液弯曲杆菌牛生物变种354 csp Campylobacter sputorum ss. Sputorum/唾液弯曲杆菌唾生物变种355 cup Campylobacter upsaliensis/乌普萨拉弯曲杆菌356 cal Candida albicans/白色假丝酵母菌357 cct Candida catenulata/链状假丝酵母菌358 cci Candida ciferrii/西弗假丝酵母菌359 cdu Candida dubliniensis/都柏林假丝酵母菌360 tca Candida famata/无名假丝酵母菌361 cgl Candida glabrata/光滑假丝酵母菌362 cgu Candida guilliermondii/季也蒙假丝酵母菌363 tha Candida haemulonii364 chu Candida humicola365 cin Candida intermedia366 cps Candida kefyr (pseudotropicalis)/乳酒假丝酵母菌367 ckr Candida krusei/克柔假丝酵母菌368 clm Candida lambica/郎比可假丝酵母菌369 clp Candida lipolytica/解脂假丝酵母菌370 clu Candida lusitaniae/葡萄牙假丝酵母菌371 cpa Candida parapsilosis/近平滑假丝酵母菌372 cal Candida paratropicalis/副热带假丝酵母菌373 cnp Candida pelliculosa/菌膜假丝酵母菌374 cps Candida pseudotropicalis/伪热带假丝酵母菌375 cpl Candida pulcherrima/铁红假丝酵母菌376 cct Candida ravauti377 cru Candida rugosa/皱褶假丝酵母菌378 can Candida sp./假丝酵母菌（念珠菌）属379 csl Candida stellatoidea/类星型假丝酵母菌380 ctn Candida tenuis381 ctr Candida tropicalis/热带假丝酵母菌382 cut Candida utilis/产朊假丝酵母菌383 cvn Candida vini/酸酒假丝酵母菌384 cvw Candida viswanathii/维斯假丝酵母菌385 cze Candida zeylanoides/涎沫假丝酵母菌386 cxa Candida, not albicans/非白色假丝酵母菌387 chp Capillaria hepatica/肝毛细线虫388 cph Capillaria philippinensis/菲律宾毛细线虫389 cai Capillaria sp./毛细线虫属390 boc Capnocytophaga (Bact.) ochracea (CDC DF-1)/黄褐二氧化碳嗜纤维菌391 ccm Capnocytophaga canimorsus (CDC DF-2)/ 狗咬二氧化碳嗜纤维菌392 ccy Capnocytophaga cynodegmi (CDC DF-2-like)/ 犬咬二氧化碳嗜纤维菌393 cgi Capnocytophaga gingivalis (CDC DF-1)/ 牙龈二氧化碳嗜纤维菌394 cgn Capnocytophaga granulosa/颗粒二氧化碳嗜纤维菌395 chc Capnocytophaga haemolytica/溶血二氧化碳嗜纤维菌396 cap Capnocytophaga sp./二氧化碳嗜纤维菌属397 cas Capnocytophaga sputigena (CDC DF-1)/ 生痰二氧化碳嗜纤维菌398 bac Capsularis sp./荚膜菌属399 cho Cardiobacterium hominis (CDC IId)/人类心杆菌400 car Cardiobacterium sp./心杆菌401 cay Caryophanon sp./显核菌属402 cmo Catonella morbi/疾病卡托菌属403 cat Catonella sp./卡托菌属404 abe CDC Coryneform Group 1405 abe CDC Coryneform Group 2406 dho CDC Coryneform Group 3407 dho CDC Coryneform Group 5408 mib CDC Coryneform Group A-4409 mib CDC Coryneform Group A-5410 caf CDC Coryneform Group ANF-1411 bre CDC Coryneform Group B-1412 bre CDC Coryneform Group B-3413 cd2 CDC Coryneform Group D-2414 bad CDC Coryneform Group E415 cf1 CDC Coryneform Group F-1416 ci2 CDC Coryneform Group F-2417 cmc CDC Coryneform Group G-1418 cg2 CDC Coryneform Group G-2419 ci1 CDC Coryneform Group I-1420 ci2 CDC Coryneform Group I-2421 cjk CDC Coryneform Group JK 422 aat CDC Coryneform-like Group 1 423 cap CDC DF-1424 ccm CDC DF-2425 ccy CDC DF-2-like426 cd3 CDC DF-3427 c4d CDC EF-1428 vho CDC EF-13429 ctg CDC EF-19430 ccn CDC EF-22431 e26 CDC EF-26432 vvu CDC EF-3433 ef4 CDC EF-4434 e4a CDC EF-4a435 e4b CDC EF-4b436 pdm CDC EF-5437 vfl CDC EF-6438 tpt CDC EF-9439 evu CDC Enteric Group 1440 efe CDC Enteric Group 10441 ehe CDC Enteric Group 11442 cda CDC Enteric Group 15443 vme CDC Enteric Group 16444 eas CDC Enteric Group 17445 lad CDC Enteric Group 41446 vho CDC Enteric Group 42447 yre CDC Enteric Group 45448 mwi CDC Enteric Group 46449 lgr CDC Enteric Group 57450 c58 CDC Enteric Group 58451 c59 CDC Enteric Group 59452 c60 CDC Enteric Group 60453 c63 CDC Enteric Group 63454 c64 CDC Enteric Group 64455 c68 CDC Enteric Group 68456 c69 CDC Enteric Group 69457 ehm CDC Enteric Group 75458 kas CDC Enteric Group 8459 tgu CDC Enteric Group 90460 pce CDC EO-1461 eo2 CDC EO-2462 eo3 CDC EO-3464 hap CDC HB-2 465 hac CDC HB-3 466 hac CDC HB-4 467 pbe CDC HB-5 468 pma CDC I 469 pdi CDC Ia 470 spf CDC Ib-1 471 spf CDC Ib-2 472 fme CDC IIa 473 fin CDC IIb 474 c2c CDC IIc 475 cho CDC IId 476 c2e CDC IIe 477 wvi CDC IIf 478 c2h CDC IIh 479 c2i CDC IIi 480 axy CDC IIIa 481 axy CDC IIIb 482 wzo CDC IIj 483 spa CDC IIk-1 484 sml CDC IIk-2 485 ssv CDC IIk-3 486 bbr CDC IV a 487 bpa CDC IVb 488 c4c CDC IVc 489 4c2 CDC IVc-2 490 c4d CDC IVd 491 oul CDC IV e 492 kki CDC M-1 493 mpp CDC M-2 494 mat CDC M-3 495 our CDC M-4 496 fod CDC M-4f 497 nwe CDC M-5 498 nni CDC M-6 499 bhm CDC NO-2 500 kde CDC TM-1 501 ppi CDC V a-1 502 ppi CDC V a-2 503 ppi CDC V a-3 504 psz CDC Vb-1506 cvb CDC Vb-3507 ade CDC Vc508 oan CDC Vd-1509 oan CDC Vd-2510 atu CDC Vd-3511 clt CDC V e-1512 for CDC V e-2513 afa CDC VI514 cda Cedecea davisae/戴氏西地西菌515 cle Cedecea lapagei/拉氏西地西菌516 cnt Cedecea neteri/奈氏西地西菌517 ced Cedecea sp./西地西菌属518 ce3 Cedecea sp. 3519 cnt Cedecea sp. 4520 ce5 Cedecea sp. 5521 otu Cellulomonas (Oersk.) turbata/浑浊纤维单胞菌522 cce Cellulomonas cellulans (O. xanthineolytica)/ 纤维化纤维单胞菌523 cel Cellulomonas sp./纤维单胞菌属524 cpo Centipeda periodontii/牙周百足菌(牙周蜈蚣菌)525 cen Centipeda sp./蜈蚣菌属526 acr Cephalosporium sp./头孢属527 cha Chaetomium sp./毛壳菌属528 cme Chilomastix mesnili/迈氏唇鞭毛虫529 chx Chilomastix sp./后辰鞭毛虫属530 chl Chlamydia sp./衣原体属531 cht Chlamydia trachomatis/沙眼衣原体532 cpn Chlamydia pneumoniae/肺炎衣原体533 cpt Chlamydia psittaci/鹦鹉热衣原体534 chd Chlamydia sp./衣原体属535 chm Chromobacterium sp./色素杆菌属536 cvi Chromobacterium violaceum/紫色色杆菌537 fgl Chryseobacterium (Flavo.) gleum (CDC IIb)/粘金黄杆菌538 fin Chryseobacterium (Flavo.) indologenes (CDC IIb)/ 产吲哚金黄杆菌539 fme Chryseobacterium (Flavo.) meningosepticum (IIa)/脑膜脓毒性金黄杆菌540 chb Chryseobacterium sp./金黄杆菌属541 clt Chryseomonas (Pseudo.) luteola (CDC V e-1)/ 浅黄金色单胞菌542 chr Chryseomonas sp./华丽单胞菌属543 ccr Chrysosporium parvum var. crescens/短小金孢子菌新月金孢544 cpr Chrysosporium parvum var. parvum/短小金孢子菌短小金孢子545 chs Chrysosporium sp./金孢子菌属546 cir Circinella sp./卷霉属547 cml Citrobacter amalonaticus/无丙二酸柠檬酸杆菌548 cbk Citrobacter braakii/布氏柠檬酸杆菌549 cdi Citrobacter diversus/差异柠檬酸杆菌550 cdl Citrobacter diversus-levinea551 cfa Citrobacter farmeri/法氏柠檬酸杆菌552 cfr Citrobacter freundii/弗氏柠檬酸杆菌553 c10 Citrobacter genomospecies 10554 c11 Citrobacter genomospecies 11555 ci9 Citrobacter genomospecies 9556 cdi Citrobacter koseri (diversus)/柯氏柠檬酸杆菌557 csk Citrobacter sedlakii/塞氏柠檬酸杆菌558 ci- Citrobacter sp./柠檬酸杆菌属559 cwe Citrobacter werkmanii/魏氏柠檬酸杆菌560 cyo Citrobacter youngae/杨氏柠檬酸杆菌561 cbn Cladophialophora bantiana/斑替枝孢霉562 cbp Cladophialophora boppii563 crr Cladophialophora carrionii564 cde Cladophialophora devriesii565 cld Cladophialophora sp./支孢瓶霉属566 cla Cladosporium sp./芽枝霉属567 cmg Clavibacter michiganense ss. Michiganense/密执安棍状杆菌密执安亚种568 clv Clavibacter sp./棍状杆菌属569 hci Clo-1570 hfn Clo-2571 csi Clonorchis sinensis/华支睾吸虫572 clc Clonorchis sp./支睾吸虫属573 cau Clostridium absonum/不同梭菌574 cab Clostridium acetobutylicum/丙酮丁醇梭菌575 cav Clostridium aminovalericum/氨基戊酸梭菌576 cag Clostridium argentinense/阿根廷梭菌577 cba Clostridium barati/霸氏梭菌578 cbe Clostridium beijerinckii/拜氏梭菌579 cbi Clostridium bifermentans/双酶梭菌580 cbo Clostridium botulinum/肉毒梭菌581 cbu Clostridium butyricum/丁酸梭菌582 ccd Clostridium cadaveris/尸毒梭菌583 cca Clostridium carnis/肉梭菌584 cch Clostridium chauvoei/气肿疽梭菌585 ccl Clostridium clostridiiforme/梭形梭菌586 ccc Clostridium cochlearium/匙形梭菌587 cdf Clostridium difficile/难辨梭菌588 cfx Clostridium fallax/谲诈梭菌589 cgh Clostridium ghoni/戈氏梭菌590 cgc Clostridium glycolicum/乙二醇梭菌591 che Clostridium haemolyticum/溶血梭菌592 chf Clostridium hastiforme/矛形梭菌593 chi Clostridium histolyticum/溶组织梭菌594 cid Clostridium indolis/吲哚梭菌595 cic Clostridium innocuum/无害芽胞梭菌596 cig Clostridium irregularis/不规则梭菌597 cli Clostridium limosum/泥渣梭菌598 cma Clostridium malenominatum/恶名梭菌599 cnx Clostridium nexile/系结梭菌600 cno Clostridium novyi/诺维梭菌601 cna Clostridium novyi type a/诺维梭菌a型602 cnb Clostridium novyi type b/诺维梭菌b型603 cou Clostridium oroticum/乳清酸梭菌604 cba Clostridium paraperfringens/副产气荚膜梭菌605 cpp Clostridium paraputrificum/类腐败梭菌606 cpe Clostridium perfringens/产气荚膜梭菌607 cpf Clostridium piliforme/毛状梭菌608 clf Clostridium putrefaciens/腐化梭菌609 cpu Clostridium putrificum/腐败梭菌610 cra Clostridium ramosum/多枝梭菌611 csa Clostridium sartagoformum/煎盘梭菌612 csc Clostridium scatologenes/粪味梭菌613 cse Clostridium septicum/败毒梭菌614 cso Clostridium sordellii/索氏梭菌615 clo Clostridium sp./梭菌属616 csn Clostridium sphenoides/楔形梭菌617 csg Clostridium sporogenes/产芽胞梭菌618 css Clostridium sporosphaeroides/球孢梭菌619 csu Clostridium subterminale/近端梭菌620 csy Clostridium symbiosum/共生梭菌621 ctt Clostridium tertium/第三梭菌622 cte Clostridium tetani/破伤风梭菌623 cim Coccidioides immitis/粗球孢子菌624 coc Coccidioides sp./球孢子菌属625 cac Comamonas (Pseudo.) acidovorans/食酸丛毛假单胞菌626 cts Comamonas (Pseudo.) testosteroni/睾丸酮丛毛单胞菌627 com Comamonas sp./丛毛单胞菌属628 ctg Comamonas terrigena/土生丛毛单胞菌629 cbc Conidiobolus coronatus/冠状耳霉630 cod Conidiobolus sp./耳霉属631 con Contaminated specimen632 cot Contracaecum sp./对盲囊[线虫]属633 cpc Coprococcus sp./粪球菌属634 cao Corynebacterium accolens/拥挤棒杆菌635 caf Corynebacterium afermentans/非发酵棒杆菌636 ci2 Corynebacterium amycolatum (CDC F-2, I-2)/ 无枝菌酸棒杆菌637 caq Corynebacterium aquaticum/水生棒杆菌638 cbv Corynebacterium bovis/牛棒杆菌639 crc Corynebacterium cystitidis/膀胱炎棒杆菌640 cdt Corynebacterium diphtheriae/白喉棒状杆菌641 req Corynebacterium equi/马红球菌642 cgm Corynebacterium glutamicum/谷氨酸棒杆菌643 ahe Corynebacterium haemolyticum/溶血隐秘杆菌644 cjk Corynebacterium jeikeium (CDC JK)/JK棒状杆菌645 cku Corynebacterium kutscheri/库氏棒杆菌646 cmc Corynebacterium macginleyi/麦氏棒杆菌647 cmt Corynebacterium matruchotii/马氏棒杆菌648 cmg Corynebacterium michiganense/密执安棍状杆菌649 cmi Corynebacterium minutissimum/极小棒杆菌650 cmy Corynebacterium mycetoides/类真菌棒杆菌651 cpi Corynebacterium pilosum/多毛棒杆菌652 cpq Corynebacterium propinquum (CDC ANF-3)/接近棒杆菌653 cpd Corynebacterium pseudodiphtheriticum/假白喉棒状杆菌654 cpb Corynebacterium pseudotuberculosis/假结核棒状杆菌655 apy Corynebacterium pyogenes/化脓隐秘杆菌656 crn Corynebacterium renale/肾棒杆菌657 cor Corynebacterium sp./棒状杆菌属658 cdp Corynebacterium sp. (diphtheroids)659 cst Corynebacterium striatum/纹带棒杆菌660 cul Corynebacterium ulcerans/溃疡棒杆菌661 cd2 Corynebacterium urealyticum (CDC D-2)/解脲棒杆菌662 cxe Corynebacterium xerosis/干燥棒杆菌663 cbt Coxiella burnetii/伯氏考克斯体(立克次体)664 cox Coxiella sp./考克斯体属665 cri Cristispira sp./脊螺旋体属666 caa Cryptococcus albidus var. albidus667 cad Cryptococcus albidus var. diffluens668 cae Cryptococcus ater669 cyr Cryptococcus cereanus670 cga Cryptococcus gastricus/胃隐球菌671 slt Cryptococcus lactativorus672 cln Cryptococcus laurentii/罗伦隐球酵母菌673 cll Cryptococcus luteolus/淡黄隐球菌674 mym Cryptococcus melibiosum675 cne Cryptococcus neoformans/新型隐球菌676 cgt Cryptococcus neoformans var. gattii/新生隐球酵母盖替变种677 cry Cryptococcus sp./隐球菌属678 ctu Cryptococcus terreus/地生隐球菌679 cug Cryptococcus uniguttulatus/指甲隐球菌680 cpv Cryptosporidium parvum/小球隐孢子虫681 coo Cryptosporidium sp./隐孢子虫属682 cbr Cunninghamella bertholletiae/灰色小克银汉霉683 cun Cunninghamella sp./小坎宁安霉属684 cur Curvularia sp./弯孢(霉)属685 ccs Cyclospora cayetanensis/圆孢子球虫686 cyc Cyclospora sp./环孢子菌属687 cyn Cylindrocarpon cyanescens688 clh Cylindrocarpon lichenicola/苔藓柱孢689 cyl Cylindrocarpon sp./柱孢属690 dei Deinococcus sp./异常球菌属691 den Dendrostilbella sp.692 dho Dermabacter hominis/人皮杆菌693 der Dermabacter sp./皮杆菌属694 drm Dermacentor sp./革蜱属695 det Dermatophilus sp./嗜皮菌属696 dep Dermatophyte/皮肤癣菌697 deb Desulfobacter sp./脱硫菌属698 del Desulfobulbus sp./脱硫叶菌属699 dec Desulfococcus sp./脱硫球菌属700 dpg Desulfomonas pigra/惰性脱硫单胞菌701 dem Desulfomonas sp./脱硫单胞菌属702 des Desulfosarcina sp./脱硫八叠球菌属703 dae Desulfovibrio desulfuricans ss. aestuarii704 dde Desulfovibrio desulfuricans ss. desulfuricans705 dev Desulfovibrio sp.脱硫弧菌属706 dpn Dialister (Bact.) pneumosintes/侵肺戴阿李斯特杆菌707 dia Dialister sp./戴阿李斯特杆菌属708 dno Dichelobacter nodosus/节瘤偶蹄形菌709 dic Dichelobacter sp./偶蹄形菌属710 dfr Dientamoeba fragilis/脆双核阿米巴711 die Dientamoeba sp./双核阿米巴属712 cdp Diphtheroides/类白喉菌713 dla Diphyllobothrium latum/阔节裂头绦虫714 dpb Diphyllobothrium sp./裂头绦虫715 dca Dipylidium caninum/犬绦虫716 dpd Dipylidium sp./复孔绦虫属717 dim Dirofilaria immitis/犬恶丝虫718 dir Dirofilaria sp./恶丝虫属719 dpi Dolosigranulum pigrum/懒惰狡诈球菌720 dol Dolosigranulum sp./狡诈球菌属721 dme Dracunculus medinensis/麦地那龙线虫722 dra Dracunculus sp./龙线虫属723 dre Drechslera sp./德氏霉属724 egr Echinococcus granulosus/细粒棘球绦虫725 emu Echinococcus multilocularis/多房棘球绦虫726 ech Echinococcus sp./棘球绦虫属727 eil Echinostoma ilocanum/伊族棘口吸虫728 ecs Echinostoma sp./棘口吸虫属729 eho Edwardsiella hoshinae/保科爱德华菌730 eic Edwardsiella ictaluri/鲶鱼爱德华菌731 edw Edwardsiella sp./爱德华菌属732 eta Edwardsiella tarda/迟钝爱德华菌733 ehc Ehrlichia canis/犬埃里希氏体734 ehf Ehrlichia chaffeensis/恰菲埃里希体735 ehs Ehrlichia sennetsu/腺热埃里希体736 ehr Ehrlichia sp./埃里希体属737 ecr Eikenella corrodens (CDC HB-1)/啮蚀艾肯菌738 eik Eikenella sp./艾肯菌属739 ebr Empedobacter (Flavo.) brevis/短稳杆菌740 emp Empedobacter sp./稳杆菌属741 enc Encephalitozoon sp./脑胞内原虫属742 ena Endolimax nana/微小内蜒阿米巴743 end Endolimax sp./内蜒阿米巴属744 eci Entamoeba coli/结肠内阿米巴745 edp Entamoeba dispar/不相称内阿米巴(迪斯帕内阿米巴) 746 egi Entamoeba gingivalis/龈内阿米巴747 eha Entamoeba hartmanni/哈特曼内阿米巴748 ehi Entamoeba histolytica/溶组织内阿米巴749 ehd Entamoeba histolytica/dispar溶组织/不相称内阿米巴750 epo Entamoeba polecki/波氏内阿米巴751 enm Entamoeba sp./内阿米巴属752 eae Enterobacter aerogenes/产气肠杆菌753 eag Enterobacter agglomerans/聚团肠杆菌(成团泛菌) 754 eam Enterobacter amnigenus/河生肠杆菌755 ea1 Enterobacter amnigenus 1/河生肠杆菌生物1型756 ea2 Enterobacter amnigenus 2/河生肠杆菌生物2型。

钩虫课件

3. 易感人群：旱地作物种植者，婴幼儿等
主界面形态生活史致病诊断流行防治
1. 普查普治，消灭传染源：驱虫（阿苯哒唑、赛特斯片等）；用铁剂纠正贫血。 2. 加强粪管和粪便无害化处理，改良耕作方式。
3. 注意个人防护：不赤脚下地，手足皮肤
可涂抹1.5%左旋咪唑硼酸酒精或15%噻苯咪唑软膏。
不同发育程度的钩虫卵：
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可寄生人体丝虫共有八种，中国只有两种：
班氏吴策线虫 Wuchereria bancrofti (班氏丝虫）
马来布鲁线虫
Brugia malayi (马来丝虫）
均寄生于人淋巴系统，引起淋巴丝虫病。
临床表现有淋巴管炎、乳糜尿、象皮肿等
动画
成虫： 1. 乳白色，细丝线状，体表光滑，长
犬钩口线虫( A. Caninum)：偶可感染人体
巴西钩口线虫( A. Braziliense)：引起皮肤幼虫移行症
主菜单
形态
生活史
致病
诊断
流行
防治
成虫： 1 2
体长1cm左右，半透明，肉红色头端向背面仰曲，具有头腺1对，咽腺3个，顶端有发达的角质口囊，内
腹侧缘有钩齿2对或板齿1对
3
雌虫尾端尖，雄虫尾端膨大为交合伞
区别点
十二指肠钩虫
美洲钩虫
大小体态口囊交合伞背肋分支交合刺
稍大
8－13mm
较小 7-11mm 呈“Ｓ“形腹侧有1对板齿展开为扁圆形近端分支2×2 末端合拢成虫虫卵
呈“Ｃ“形腹侧前缘有2对钩齿展开为圆形远端分支2×3 长鬃状，末端分开返回
虫体前端的三种单细胞腺
1 头腺：一对，分泌抗凝血酶、乙酰

钩虫、蛲虫

2、成虫所致病变
成虫为主要致病阶段贫血(anemia)：慢性失血，（1）贫血(anemia)：慢性失血，铁和蛋白质不断耗损，再加上患者营养不良，质不断耗损，再加上患者营养不良，血红蛋白合成速度比细胞新生速度慢，红蛋白合成速度比细胞新生速度慢，则使红细胞体积变小、着色变浅，使红细胞体积变小、着色变浅，引起低色素小细胞性贫血。贫血症状：色素小细胞性贫血。贫血症状：皮肤蜡粘膜苍白、眩晕、乏力甚至心慌等。黄、粘膜苍白、眩晕、乏力甚至心慌等。偶尔会出现消化道大出血。偶尔会出现消化道大出血。
5 ）此外钩蚴有 “ 迁延移行现象 ” ：指十二指肠此外钩蚴有“ 迁延移行现象” 钩虫的钩蚴经皮肤感染人体后，钩虫的钩蚴经皮肤感染人体后，部分在进入小肠之前，可滞留于某些组织中达很长时间，肠之前，可滞留于某些组织中达很长时间，此虫体发育缓慢或“暂停发育” 时，虫体发育缓慢或“暂停发育”，在受到某些刺激后，才陆续到达小肠发育成熟。些刺激后，才陆续到达小肠发育成熟。犬钩虫也存在上述情况，也存在上述情况，但未发现美洲钩虫迁延移行现象。现象。 6）还有试验证明某些动物（猪、狮、犬、灵猫、还有试验证明某些动物（灵猫、猩猩、犀牛）可作为钩虫的转续宿主转续宿主。猴、猩猩、犀牛）可作为钩虫的转续宿主。
三、致病
人体感染钩虫后是否出现临床症状与钩蚴侵入皮肤的数量、成虫在小肠寄生的数量有关，肤的数量、成虫在小肠寄生的数量有关，也与人体的健康状况、营养条件及免疫力有关。体的健康状况、营养条件及免疫力有关。钩虫感染（ infection）钩虫感染（ hookworm infection）：在粪便中可检获虫卵，但无任何临床症状。检获虫卵，但无任何临床症状。钩虫病（ disease）既有钩虫感染, 钩虫病（hookworm disease）：既有钩虫感染,又有临床症状和体征。有临床症状和体征。钩虫病的临床表现可分三个时期，钩虫病的临床表现可分三个时期，即由幼虫引起皮肤（或粘膜）侵袭期、肺部移行期移行期和成虫在肠皮肤（或粘膜）侵袭期、肺部移行期和成虫在肠寄生期（此期危害最严重）。道寄生期（此期危害最严重）。

寄生虫 (3)

1
1
幼虫所致病变及症状
钩蚴性皮炎 dermatitis caused by hookworm larvae 感染期蚴钻入皮肤后数十分钟内患者局部皮肤即可有针刺烧灼和奇痒感, 出现 1-2日内出现红肿及水疱充血斑点或丘疹搔破后可有浅黄色液体流出若有继发细菌感染则形成脓疱最后经结痂脱皮而愈俗称为“粪毒”或“地痒疹”.能自愈病程2-3周 2 呼吸道症状(symptoms in respiratory system) 急性钩蚴感染钩蚴移行至肺穿破微血管可引起出血及炎症细胞浸润患者出现阵发性咳嗽血痰及哮喘
2.成虫所致病变及症状成虫为主要致病阶段 1 贫血(anemia) 慢性失血铁和蛋白质不断耗损再加上患者营养不良血红蛋白合成速度比细胞新生速度慢则使红细胞体积变小着色变浅引起低色素小细胞性贫血贫血症状皮肤蜡黄粘膜苍白眩晕乏力甚至心慌等
引起慢性失血的原因 a. 吸血以钩齿或板齿咬附肠粘膜吸血 b.渗血钩虫吸血时头腺分泌抗凝素使伤口血液不易凝固自咬附部位粘膜伤口渗出的血液量与吸血量大致相当 c.虫体不断更换咬附部位原伤口在凝血前仍可继续渗出少量血液 d.虫体活动造成组织血管的损伤也可引起血液的流失
毛首鞭形线虫
Trichuris trichiura
毛首鞭形线虫简称鞭虫成虫寄生于人体盲肠引起鞭虫病 trichuriasis
一. 形态 1.成虫外形似马鞭前端细长约占虫体长的3/5 后端明显膨大雌虫尾端钝圆雄虫尾端向腹面呈环状卷曲末端有一根交合刺
虫卵 1 黄褐色纺锤形 2 卵壳较厚内有脂层中为壳质层外为卵黄膜两端各具一个透明的盖塞由称透明栓 3 卵内含有一个卵细胞

犬钩虫(Ancylostoma caninum)天冬氨酸蛋白酶基因的克隆和在大肠杆菌中的表达

犬钩虫(Ancylostoma caninum)天冬氨酸蛋白酶基因的克隆和在大肠杆菌中的表达杨玉荣;韦华【期刊名称】《中国人兽共患病学报》【年(卷),期】2007(23)12【摘要】目的对犬钩虫(Ancylostoma caninum)天冬氨酸蛋白酶Asp基因进行了克隆,鉴定,并在原核系统中进行表达.方法以犬钩虫总RNA为模板,用RT-PCR法对犬钩虫天冬氨酸蛋白酶Asp基因进行扩增,获得的Asp cDNA产物克隆进pMD-18T载体,用PCR筛选阳性克隆.碱裂解法进行质粒提取,单、双酶切进行鉴定并测序.将测序正确的阳性克隆进行双酶切,构建到表达载体pET-32a中,将此重组质粒先转化到E.coli DH5a内,提取质粒,酶切鉴定阳性,再转化入表达宿主 E.coli BL21(DE3)菌株内,对转化菌株用IPTG进行诱导培养.收集培养液,破菌、离心,上清进行SDS-PAGE,通过电转移将胶中蛋白转到硝酸纤维素膜上后进行免疫印迹分析.结果电泳发现转化了重组质粒的菌株有表达蛋白,所表达蛋白相对分子量为40kDa,抗体检测有特异条带大小为40kDa.结论成功进行了犬钩虫天冬氨酸蛋白酶Asp 基因的克隆表达.【总页数】4页(P1222-1225)【作者】杨玉荣;韦华【作者单位】厦门大学生命科学学院寄生动物研究室,厦门,361005;厦门大学生命科学学院寄生动物研究室,厦门,361005【正文语种】中文【中图分类】R383【相关文献】1.犬钩虫(Ancylostoma caninum)daf-1基因的克隆和表达鉴定 [J], 乔莹;杨玉荣2.NADPH依赖的乙酰乙酰辅酶A还原酶基因phbB的克隆和在大肠杆菌中的表达[J], 袁辉;华子春3.酵母pac-1基因的克隆、序列分析和在大肠杆菌中的高效表达及活性测定 [J], 步威;孙洁霖;杨希才4.人内皮抑素基因的克隆和在大肠杆菌中的表达 [J], 谢月英;孙大兴5.白念珠菌天冬氨酸蛋白酶基因SAP2在大肠杆菌中的表达及产物纯化 [J], 李蕾;仇萌;邹先彪因版权原因，仅展示原文概要，查看原文内容请购买。

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ｌｙｉｎｔｏｐＭＤ－１８Ｔｖｅｃｔｏｒ．ＡｆｔｅｒＰＣＲｓｅｌｅｃｔｉｏｎ，ｅｎｚｙｍｅｄｉｇｅｓｔｉｏｎ，ａｎｄｓｅｑｕｅｎｃｉｎｇ．Ｔｈｅｐｏｓｉｔｉｖｅａｎｄｒｉｇｈｔｓｅｑｕｅｎｃｅｏｆｐｌａｓｍｉｄ
关键词：犬钩虫；天冬氨酸蛋白酶；克隆；表达中图分类号：Ｒ３８３文献标识码：Ａ
Ｃｌｏｎｉｎｇ，ｅｘｐｒｅｓｓｉｏｎａｎｄｉｄｅｎｔｉｆｉｃａｔｉｏｎｏｆａｓｐａｒｔｉｃｐｒｏｔｅａｓｅｇｅｎｅ
方法以犬钩虫总ＲＮＡ为模板，用ＲＴ — ＰＣＲ法对犬钩虫天冬氨酸蛋白酶Ａｓｐ基因进行扩增，获得的ＡｓｐｃＤＮＡ产物克隆进
ｐＭＤ－１８Ｔ载体，用ＰＣＲ筛选阳性克隆。碱裂解法进行质粒提取，单、双酶切进行鉴定并测序。将测序正确的阳性克隆进行双酶切，构建到表达载体ｐＥＴ一３２ａ中，将此重组质粒先转化到Ｅ．ｃｏｌｉＤＨ５ａ内，提取质粒，酶切鉴定阳性，再转化入表达宿主ＥｃｏｌｉＢＬ２１（ＤＥ３）菌株内，对转化菌株用ＩＰＴＧ进行诱导培养。收集培养液，破菌、离心，上清进行ＳＤＳ－ＰＡＧＥ，通过电转移将胶中蛋白转到硝酸纤维素膜上后进行免疫印迹分析。结果电泳发现转化了重组质粒的菌株有表达蛋白，所表达蛋白相对分子量为４０ｋＤａ，抗体检测有特异条带大小为４０ｋＤａ。结论成功进行了犬钩虫天冬氨酸蛋白酶Ａｓｐ基因的克隆表达。
维普共ｌ患病ｏ学ｓ报ＣｈｎｅｅＪｏｕｎａｏｆＺｏｎｏｅｓ
２００７，２３（１２）
文章编号：１００２ —２６９４（２００７）１２ —１２２２ —０４
ＡＢＳＴＲＡＣＴ；Ｃｌｏｎｉｎｇ，ｅｘｐｒｅｓｓｉｎｇａｎｄｉｄｅｎｔｉｆｙｔｈｅａｓｐａｒｔｉｃｐｒｏｔｅａｓｅｇｅｎｅｆｒｏｍＡｎｃｙｌｏｓｔｏｍａｃａｎｉｎｕｍｆｏｒｔｈｅｆｕｒｔｈｅｒｓｔｕｄ — ｙｉｎｇｉｎｔｈｅａｐｐｌｉｃａｔｉｏｎｉｎｔｈｅｄｉａｇｎｏｓｉｓｏｆＡｎｃｙｌｏｓｔｏｍａａｓｗｅｌｌａｓｆｏｒｔｈｅｄｅｖｅｌｏｐｍｅｎｔｏｆａｎｔｉ－ｈｏｏｋｗｏｒｍｖａｃｃｉｎｅ．Ｔｈｅｇｅｎｅｅｎｃｏ — ｄｉｎｇａｓｐａｒｔｉｃｐｒｏｔｅａｓｅｗａｓａｍｐｌｉｆｉｅｄｆｒｏｍｔｈｅｔｏｔａｌＲＮＡｂｙｕｓｉｎｇＲＴ— ＰＣＲｔｅｃｈｎｉｑｕｅ．Ｔｈｅａｍｐｌｉｆｉｅｄｐｒｏｄｕｃｔｗａｓｃｌｏｎｅｄｉｎｉｔｉａｌ —
犬钩虫（Ａｎｃｙｌｏｓｔｏｍａｃａｎｉｎｕｍ）天冬氨酸蛋白酶基因的克隆和在大肠杆菌中的表达
杨玉荣，韦华
摘要：目的对犬钩虫（Ａｎｃｙｌｏｓｔｏｍａｃａｎｉｎｕｍ）天冬氨酸蛋白酶Ａｓｐ基因进行了克隆，鉴定，并在原核系统中进行表达。
ｗａｓｄｉｇｅｓｔｅｄｂｙｅｎｚｙｍｅａｎｄｌｉｇａｔｅｄｗｉｔｈｄｉｇｅｓｔｅｄｅｘｐｒｅｓｓｉｏｎｖｅｃｔｏｒｐＥＴ一３２ａｂｙｌｉｇａｓｅ，ａｎｄｔｈｅｎｔｒａｎｓｆｏｒｍｅｄｔｈｅｃｏｎｓｔｒｕｃｔｉｎｔｏ
ｏｆＡｎｃｙｌｏｓｔｏｍａｃａｎｉｎｕｍｉｎＥ．ｃｏｌｉ
ＹＡＮＧＹｕ — ｒｏｎｇ．ＷＥＩＨｕａ
（ＰａｒａｓｉｔｅＲｅｓｅａｒｃｈＬａｂ，ＬｉｆｅＳｃｉｅｎｃｅＳｃｈｏｏｌ，ＸｉａｍｅｎＵｎｉｖｅｒｓｉｔｙ，Ｘｉａｍｅｎ３６１００５，Ｃｈｉｎａ）