Pizotifen malate_5189-11-7_DataSheet_MedChemExpress
Pepperl+Fuchs NJ5-18GK-SN 漏Magnetic感应传感器说明书
17-01-24 15:36D a t e o f i s s u e : 2017-01-24250929_e n g .x m lInstructionManual electrical apparatus for hazardous areas Device category 1Gfor use in hazardous areas with gas, vapour and mist EC-T ype Examination CertificateCE marking ATEX marking ¬ II 1G Ex ia IIC T6…T1 G aThe Ex-related marking can also be printed on the enclosed label.Standards EN 60079-0:2012+A11:2013 EN 60079-11:2012 Ignition protection "Intrinsic safety"Use is restricted to the following stated conditions Appropriate typeNJ 5-18GK-SN...Effective internal inductivity C i ≤ 120 nF ; a cable length of 10 m is considered.Effective internal inductance L i≤ 200 µH ; a cable length of 10 m is considered.G eneralThe apparatus has to be operated according to the appropriate data in the data sheet and in this instruction manual. The EU-type examination certificate has to beobserved. The special conditions must be adhered to! The ATEX directive and there-fore the EU-type-examination certificates generally apply only to the use of electrical apparatus under atmospheric conditions.The device has been checked for suitability for use at ambient temperatures of > 60 °C by the named certification authority. The surface temperature of the device remains within the required limits.For the use of apparatus outside of atmospheric conditions, a reduction of the per-missible minimum ignition energies may need to be considered.Ambient temperatureDetails of the correlation between the type of circuit connected, the maximum per-missible ambient temperature, the temperature class, and the effective internal reac-tance values can be found on the EC-type examination certificate. Note: Use the temperature table for category 1 The 20 % reduction in accordance with EN 1127-1 has already been applied to the temperature table for category 1.Installation, commissioningLaws and/or regulations and standards governing the use or intended usage goal must be observed. The intrinsic safety is only assured in connection with an appro-priate related apparatus and according to the proof of intrinsic safety. The associated apparatus must satisfy the requirements of category ia. Because of the risk of igni-tion, which can occur due to faults and/or transient currents in the equipotential bonding system, galvanic isolation is preferable in the supply and signal circuits. Associated apparatus without electrical isolation can only be used if the correspond-ing requirements of IEC 60079-14 are satisfied. If the Ex-related marking is printed only on the supplied label, then this must be attached in the immediate vicinity of the sensor. The sticking surface for the label must be clean and free from grease. The attached label must be legible and indelible, including in the event of possible chem-ical corrosion.Maintenance No changes can be made to apparatus, which are operated in hazardous areas.Repairs to these apparatus are not possible.Special conditionsProtection from mechanical dangerWhen using the device in a temperature range of -60 °C to -20 °C, protect the sensor against the effects of impact by installing an additional enclosure. The information regarding the minimum ambient temperature for the sensor as provided in the datasheet must also be observed.Electrostatic chargeWhen used in group IIC non-permissible electrostatic charges should be avoided on the plastic housing parts. Avoid electrostatic charges that can cause electrostatic dis-charge when installing or operating the device. Information on electrostatic hazards can be found in the technical specification IEC/TS 60079-32-1.Degree of protection required when installing connecting componentsThe connecting parts of the sensor must be set up in such a way that degree of pro-tection IP20, in accordance with lEC 60529, is achieved as a minimum.R e l e a s e d a t e : 2017-01-24 15:36D a t e o f i s s u e : 2017-01-24250929_e n g .x m lInstructionManual electrical apparatus for hazardous areas Device category 2Gfor use in hazardous areas with gas, vapour and mist EC-T ype Examination CertificateCE marking ATEX marking ¬ II 1G Ex ia IIC T6…T1 G aThe Ex-related marking can also be printed on the enclosed label.Standards EN 60079-0:2012+A11:2013 EN 60079-11:2012 Ignition protection "Intrinsic safety"Use is restricted to the following stated conditions Appropriate typeNJ 5-18GK-SN...Effective internal inductivity C i≤ 120 nF ; a cable length of 10 m is considered.Effective internal inductance L i ≤ 200 µH ; a cable length of 10 m is considered.G eneralThe apparatus has to be operated according to the appropriate data in the data sheet and in this instruction manual. The EU-type examination certificate has to beobserved. The special conditions must be adhered to! The ATEX directive and there-fore the EU-type-examination certificates generally apply only to the use of electrical apparatus under atmospheric conditions.The device has been checked for suitability for use at ambient temperatures of > 60 °C by the named certification authority. The surface temperature of the device remains within the required limits.For the use of apparatus outside of atmospheric conditions, a reduction of the per-missible minimum ignition energies may need to be considered.Maximum permissible ambient temperature T amb Details of the correlation between the type of circuit connected, the maximum per-missible ambient temperature, the temperature class, and the effective internal reac-tance values can be found on the EC-type examination certificate.Installation, commissioningLaws and/or regulations and standards governing the use or intended usage goal must be observed. The intrinsic safety is only assured in connection with an appro-priate related apparatus and according to the proof of intrinsic safety. If the Ex-related marking is printed only on the supplied label, then this must be attached in the imme-diate vicinity of the sensor. The sticking surface for the label must be clean and free from grease. The attached label must be legible and indelible, including in the event of possible chemical corrosion.Maintenance No changes can be made to apparatus, which are operated in hazardous areas.Repairs to these apparatus are not possible.Special conditionsProtection from mechanical dangerWhen using the device in a temperature range of -60 °C to -20 °C, protect the sensor against the effects of impact by installing an additional enclosure. The information regarding the minimum ambient temperature for the sensor as provided in the datasheet must also be observed.Degree of protection required when installing connecting componentsThe connecting parts of the sensor must be set up in such a way that degree of pro-tection IP20, in accordance with lEC 60529, is achieved as a minimum.17-01-24 15:36D a t e o f i s s u e : 2017-01-24250929_e n g .x m lInstructionManual electrical apparatus for hazardous areasDevice category 3G (nA) for use in hazardous areas with gas, vapour and mistX CE marking ATEX marking ¬ II 3G Ex nA IIC T6 GcThe Ex-related marking can also be printed on the enclosed label.Standards EN 60079-0:2012+A11:2013, EN 60079-15:2010 Ignition protection category "n"Use is restricted to the following stated conditionsG eneralThe apparatus has to be operated according to the appropriate data in the data sheet and in this instruction manual. The data stated in the data sheet are restricted by this operating instruction! The special conditions must be observed!Installation, commissioningLaws and/or regulations and standards governing the use or intended usage goal must be observed. If the Ex-related marking is printed only on the supplied label, then this must be attached in the immediate vicinity of the sensor. The sticking surface for the label must be clean and free from grease. The attached label must be legible and indel-ible, including in the event of possible chemical corrosion.MaintenanceNo changes can be made to apparatus, which are operated in hazardous areas.Repairs to these apparatus are not possible.Special conditionsMinimum series resistance R V A minimum series resistance R V is to be provided between the power supply voltage and the proximity switch in accordance with the following list. This can also be assured by using a switch amplifier.Maximum operating voltage U BmaxThe maximum permissible operating voltage UB max is restricted to the values in the following list. T olerances are not permissible.Maximum permissible ambient temperature T Umax Values can be obtained from the following list, depending on the max. operating voltage Ub max and the minimum series resistance Rv. at U Bmax =9 V , R V =562 Ω58 °C (136.4 °F) using an amplifier in accordance with EN 60947-5-6 58 °C (136.4 °F)Protection from mechanical danger The sensor must not be exposed to ANY FORM of mechanical danger.Protection from UV lightThe sensor and the connection cable must be protected from damaging UV-radiation. This can be achieved when the sensor is used in internal areas.Protection of the connection cable The connection cable must be prevented from being subjected to tension and torsional loading.Protection against transients Ensure transient protection is provided and that the maximum value of the transient pro-tection (140% of 85 V) is not exceeded.Material selection accessoriesWhen selecting accessories, ensure that the material allows the temperature of the enclosure to rise to up to 70 °C.R e l e a s e d a t e : 2017-01-24 15:36D a t e o f i s s u e : 2017-01-24250929_e n g .x m lInstructionManual electrical apparatus for hazardous areas Device category 3G (ic) for use in hazardous areas with gas, vapour and mistXCE marking ATEX marking ¬ II 3G Ex ic IIC T6…T1 GcThe Ex-related marking can also be printed on the enclosed label.StandardsEN 60079-0:2012+A11:2013 EN 60079-11:2012 Ignition protection category "ic"Use is restricted to the following stated conditions Effective internal inductivity C i≤ 120 nF ; a cable length of 10 m is considered.Effective internal inductance L i≤ 200 µH ; A cable length of 10 m is considered.G eneralThe apparatus has to be operated according to the appropriate data in the data sheet and in this instruction manual. The data stated in the data sheet are restricted by this operating instruction! The special conditions must be observed! The ATEX Directive applies only to the use of apparatus under atmospheric conditions.If you use the device outside atmospheric conditions, consider that the permissible safety parameters should be reduced.Installation, commissioningLaws and/or regulations and standards governing the use or intended usage goal must be observed. The sensor must only be operated with energy-limited circuits, which satisfy the requirements of IEC 60079-11. The explosion group complies with the connected, supplying, power limiting circuit. If the Ex-relevant identification is printed exclusively on the adhesive label provided, this label must be affixed in the immediate vicinity of the sensor! The background surface to which the adhesivelabel is to be applied must be clean and free from grease! The applied label must be dura-ble and remain legible, with due consideration of the possibility of chemical corro-sion!Maintenance No changes can be made to apparatus, which are operated in hazardous areas.Repairs to these apparatus are not possible.Special conditionsfor Pi=34 mW, Ii=25 mA, T6 70 °C (158 °F) for Pi=34 mW, Ii=25 mA, T5 85 °C (185 °F) for Pi=34 mW, Ii=25 mA, T4-T1 100 °C (212 °F) for Pi=64 mW, Ii=25 mA, T6 69 °C (156.2 °F) for Pi=64 mW, Ii=25 mA, T5 84 °C (183.2 °F) for Pi=64 mW, Ii=25 mA, T4-T1 100 °C (212 °F) for Pi=169 mW, Ii=52 mA, T6 51 °C (123.8 °F) for Pi=169 mW, Ii=52 mA, T5 66 °C (150.8 °F) for Pi=169 mW, Ii=52 mA, T4-T1 80 °C (176 °F) for Pi=242 mW, Ii=76 mA, T6 39 °C (102.2 °F) for Pi=242 mW, Ii=76 mA, T5 54 °C (129.2 °F) for Pi=242 mW, Ii=76 mA, T4-T1 61 °C (141.8 °F)Protection from mechanical danger The sensor must not be mechanically damaged.When used in the temperature range below -20 °C the sensor should be protected from knocks by the provision of an additional housing.Connection partsThe connection parts are to be installed, such that a minimum protection class of IP20 is achieved, in accordance with IEC 60529.17-01-24 15:36D a t e o f i s s u e : 2017-01-24250929_e n g .x m lInstructionManual electrical apparatus for hazardous areas Device category 1Dfor use in hazardous areas with combustible dust EC-T ype Examination CertificateCE marking ATEX marking ¬ II 1D Ex ia IIIC T135°C DaThe Ex-related marking can also be printed on the enclosed label.Standards EN 60079-0:2012+A11:2013 EN 60079-11:2012Ignition protection "Intrinsic safety" Use is restricted to the following stated condi-tionsAppropriate typeNJ 5-18GK-SN...Effective internal inductivity C i ≤ 120 nF ; a cable length of 10 m is considered.Effective internal inductance L i≤ 200 µHA cable length of 10 m is considered.G eneralThe apparatus has to be operated according to the appropriate data in the data sheet and in this instruction manual. The EU-type examination certificate has to beobserved. The ATEX directive and therefore the EU-type-examination certificates generally apply only to the use of electrical apparatus under atmospheric conditions.The device has been checked for suitability for use at ambient temperatures of > 60 °C by the named certification authority. The surface temperature of the device remains within the required limits.For the use of apparatus outside of atmospheric conditions, a reduction of the per-missible minimum ignition energies may need to be considered.Permissible ambient temperature rangeDetails of the correlation between the type of circuit connected, the maximum per-missible ambient temperature, the surface temperature, and the effective internal reactance values can be found on the EC-type-examination certificate. The maxi-mum permissible ambient temperature of the data sheet must be noted, in addition, the lower of the two values must be maintained.Installation, commissioningLaws and/or regulations and standards governing the use or intended usage goal must be observed. The intrinsic safety is only assured in connection with an appro-priate related apparatus and according to the proof of intrinsic safety. If the Ex-related marking is printed only on the supplied label, then this must be attached in the imme-diate vicinity of the sensor. The sticking surface for the label must be clean and free from grease. The attached label must be legible and indelible, including in the event of possible chemical corrosion.Maintenance No changes can be made to apparatus, which are operated in hazardous areas.Repairs to these apparatus are not possible.Special conditionsProtection from mechanical dangerWhen using the device in a temperature range of -60 °C to -20 °C, protect the sensor against the effects of impact by installing an additional enclosure. The information regarding the minimum ambient temperature for the sensor as provided in the datasheet must also be observed.Electrostatic chargeAvoid electrostatic charges that can cause electrostatic discharge when installing or operating the device. Information on electrostatic hazards can be found in the techni-cal specification IEC/TS 60079-32-1. Do not attach the nameplate provided in areas where electrostatic charge can build up.Degree of protection required when installing connecting componentsThe connecting parts of the sensor must be set up in such a way that degree of pro-tection IP20, in accordance with lEC 60529, is achieved as a minimum.R e l e a s e d a t e : 2017-01-24 15:36D a t e o f i s s u e : 2017-01-24250929_e n g .x m lInstructionManual electrical apparatus for hazardous areas Device category 3D for use in hazardous areas with combustible dust CertificateCE marking ATEX marking ¬ II 3D Ex tc IIIC T80°C DcThe Ex-related marking can also be printed on the enclosed label.Standards EN 60079-0:2012+A11:2013, EN 60079-31:2014Protection by enclosure "tc" Some of the information in this instruction manual is more specific than the information provided in the datasheet.G eneralThe corresponding datasheets, declarations of conformity, EC-type examination certifi-cates, certifications, and control drawings, where applicable (see datasheets), form an integral part of this document. These documents can be found at . The maximum surface temperature of the device was determined without a layer of dust on the apparatus. Some of the information in this instruction manual is more specific than the information provided in the datasheet.Installation, commissioningLaws and/or regulations and standards governing the use or intended usage goal must be observed. The adhesive label provided must be affixed in the immediate vicinity of the sensor! The surface to which the label is applied must be clean, flat and free from grease! The affixed adhesive label must be readable and durable, taking account of the possibility of chemical corrosion!MaintenanceNo changes can be made to apparatus, which are operated in hazardous areas.Repairs to these apparatus are not possible.Special conditionsMinimum series resistance R V A minimum series resistance RV is to be provided between the power supply voltage and the proximity switch in accordance with the following list. This can also be assured by using a switch amplifier.Maximum operating voltage U BmaxThe maximum permissible operating voltage UBmax must be restricted to the values given in the following list. T olerances are not permitted.Maximum permissible ambient temperature T Umax Values can be obtained from the following list, depending on the max. operating voltage Ub max and the minimum series resistance Rv. at U Bmax =9 V, R V =562 Ω58 °C (136.4 °F) using an amplifier in accordance with EN 60947-5-6 331 KProtection from mechanical danger The sensor must not be exposed to ANY FORM of mechanical danger.Protection from UV lightThe sensor and the connection cable must be protected from damaging UV-radiation. This can be achieved when the sensor is used in internal areas.Protection of the connection cable The connection cable must be prevented from being subjected to tension and torsional loading.Electrostatic chargeAvoid electrostatic charges that can cause electrostatic discharge when installing or operating the device. Information on electrostatic hazards can be found in the technical specification IEC/TS 60079-32-1. Do not attach the nameplate provided in areas where electrostatic charge can build up.。
RayBio
RayBio® Mouse RANTES IQELISAKitCatalog #: IQM-RANTESUser ManualLast revised August 23, 2021Caution:Extraordinarily useful information enclosedISO 13485 Certified3607 Parkway Lane, Suite 100Norcross, GA 30092 Tel: 1-888-494-8555 (Toll Free) or 770-729-2992, Fax:770-206-2393Web: , Email: *******************RayBiotech, Inc.________________________________________RayBio® Mouse RANTES IQELISA Kit ProtocolTable of ContentsSection Page # I.Introduction3II.Reagents3III.Storage3IV.Additional Materials Required4V.Reagent Preparation4VI.Assay Procedure5VII.Assay Procedure Summary7VIII.Calculation of ResultsA. Typical DataB. Sensitivity and Recovery 8 9 9IX.Troubleshooting Guide10I. INTRODUCTIONThe RayBio®I mmuno Q uantitative E nzyme L inked I mumuno S orbent A ssay (IQELISA) is an innovative new assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of lower sample volumes while also providing more sensitivity. The RayBio® Mouse RANTES IQELISA Kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of Mouse RANTES in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Mouse RANTES coated on a 96-well PCR plate. Standards and samples are pipetted into the wells and RANTES present in a sample is bound to the wells by the immobilized antibody. The wells are washed and a detection affinity molecule is added to the plates. After washing away unbound detection affinity molecule, primers and a PCR master mix are added to the wells and data is collected using qPCR. C t values obtained from the qPCR are then used to calculate the amount of antigen contained in each sample, where lower C t values indicate a higher concentration of antigen.II. REAGENTS1.RANTES PCR Plate: 96-well PCR plate coated with anti-Mouse RANTES2.PCR Plate film3.Wash Buffer I Concentrate (20x): 25 ml of 20x concentrated solution4.Standards: 2 vials of recombinant Mouse RANTES5.Assay Diluent A: 30 ml diluent buffer, 0.09% sodium azide as preservative.6.Assay Diluent B: 15 ml of 5x concentrated buffer.7.Detection Antibody for RANTES: 2 vials of a concentrated solution of anti-MouseRANTES affinity reagent8.IQELISA Detection Reagent: 1.4ml of a 10x concentrated stock9.Primer Solution: 1.7ml vial10.PCR Master Mix: 1.2ml vial11.PCR Preparation buffer: 1ml vial of 10x concentrated buffer12.Final Wash Buffer: 10 ml vial of 10x concentrated bufferIII. STORAGEMay be stored for up to 6 months at 2°to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.IV. ADDITIONAL MATERIALS REQUIRED1.Real-time PCR instrument, Bio-Rad recommended2.Precision pipettes to deliver 2µl to 1 ml volumes.3.Adjustable 1-25 ml pipettes for reagent preparation.4.100 ml and 1 liter graduated cylinders.5.Absorbent paper.6.Distilled or deionized water.7.Log-log graph paper or computer and software for data analysis.8.Tubes to prepare standard or sample dilutions.9.Heating block or water bath capable of 80°CV. REAGENT PREPARATION1.Bring wash buffer, samples, assay diluents, and PCR plate to room temperature (18 - 25°C) before use. PCR master mix and Primer solution should be kept at 4°C at all times.2.Sample dilution: If your samples need to be diluted, 1x Assay Diluent B should be usedfor dilution of serum/plasma samples. Assay Diluent A maybe used in place if significant matrix affects are seen.Suggested dilution for normal serum/plasma: 20 fold*.*Please note that levels of the target protein may vary between different specimens.Optimal dilution factors for each sample must be determined by the investigator.3.Assay Diluent B should be diluted 5-fold with deionized water.4.Briefly spin the Detection Antibody vial before use. Add 25 µl of 1X Assay Diluent B intothe vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4°C for 5 days). This concentrate should be diluted 80-fold with 1X Assay Diluent B and used in step 4 of the Assay Procedure.5.PCR preparation buffer should be transferred to a 15mL tube and diluted with 9mL ofdeionized or distilled water before use.6.Final Wash Buffer should be transferred to a 15mL tube and diluted with 9mL ofdeionized or distilled water for every 1mL of 10x concentrate used before use.7.Preparation of standard: Preparation of standard: Briefly spin a vial of Standard. Add400 µl 1x Assay Diluent into the vial of Standard to prepare a 50 ng/ml standard.Dissolve the powder thoroughly by a gentle mix. Add 4 µl RANTES standard (50 ng/ml) from the vial of Standard, into a tube with 996 µl 1x Assay Diluent B to prepare a 200 pg/ml standard solution. Pipette 300 µl 1x Assay Diluent B into each tube. Use the 200 pg/ml standard solution to produce a dilution series (shown below). Mix each tubethoroughly before the next transfer. 1x Assay Diluent B serves as the zero standard (0 pg/ml).996 µl + 4 µl100 µl+ 300 µl100 µl+ 300 µl100 µl+ 300 µl100 µl+ 300 µl100 µl+ 300 µl100 µl+ 300 µl2000 pg/ml500pg/ml125pg/ml31.25pg/ml7.813pg/ml1.953pg/ml0.488pg/mlpg/ml8.If the Wash Buffer Concentrate (20x) contains visible crystals, warm to roomtemperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.9.Prepare the IQELISA detection reagent by calculating how much will be needed. Thismay be accomplished by multiplying the number of wells to be assayed by the volume you plan to use per well. Once the volume of IQELISA detection reagent is known,prepare the reagent by diluting it 1:10 with deionized water and mixing thoroughly.VI. ASSAY PROCEDURE1.Bring all reagents and samples to room temperature (18 - 25°C) before use. It isrecommended that all standards and samples be run in triplicate. Partial plate runs may be accomplished by cutting the PCR plate into the desired number of strips using a pair of sturdy scissors, wire cutters, or shears. The remainder may be saved and used for a later date. If this is done, the PCR Plate Film should also be cut to a suitable size.2.Add 10-25µl of each standard (see Reagent Preparation step 2) and sample intoappropriate wells. Volumes should be consistent between all wells, samples, andstandards. As little as 10µL can be used if sample volume is limited, however thisincreases the chance of technical error. Ensure there are no bubbles present at thebottom of the wells. Dislodge any bubbles with gentle tapping or with a pipette tip being careful not to contact the sides or bottom of the well. Cover well and incubate for 2.5hours at room temperature or overnight at 4°C with gentle shaking.3.Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each wellwith Wash Buffer (100 µl) using a multi-channel Pipette or autowasher. Completeremoval of liquid at each step is essential to good performance. After the last wash,remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.4.Add 25 µl of prepared Detection Antibody (Reagent Preparation step 4) to each well.Incubate for 1 hour at room temperature with gentle shaking.5.Discard the solution. Repeat the wash as in step 3.6.Add 25µL of prepared IQELISA detection reagent and incubate 1 hour with rocking(Reagent Preparation step 9)7.Discard the solution. Repeat the wash as in step 3.8.Add 100µL of Final wash buffer to each well and incubate for 5 minutes with rocking.Remove the solution from each well and repeat an additional 2x.9.Add 100µL of 1x PCR preparation buffer to each well and incubate with rocking for 5minutes before removing the buffer. Blot the plate after the buffer is removed to ensure complete removal of the buffer.10.Add 15µL of the Primer solution to each well of the plate. At this stage the plate can becovered and stored at -20°C for use the next day if needed.11.Add 10µL of PCR Master Mix to each well and pipette thoroughly to mix the well (atleast 3x up and down).12.Cover the plate with the supplied PCR Plate Film, taking care to insure the film iscompletely and even pressed onto the plate, creating an air tight seal around each well of the plate.13.Place the plate into a real-time PCR instrument using a FITC compatible wave length fordetection with the following settings for cycling1.3 minute activation at 95°C2.10 seconds 95°C denaturation3.25 seconds 62°C annealing/extension4.Repeat steps 2 and 3 29xVII. ASSAY PROCEDURE SUMMARY1.Prepare all reagents, samples and standards as instructed.2.Add 25µl standard or sample to each well.Incubate 2.5 hours at room temperature or overnight at 4°C.3.Add 25µl Detection Antibody to each well.Incubate 1 hour at room temperature.4.Add 25µL of IQELISA Detection Reagent to each well. Incubate 1 hour5.Add 15µl Primer solution and 10µL of PCR master mix to each well6.Run real-time PCRVIII. CALCULATION OF RESULTSThe primary data output of the IQELISA kit is C t values. These values represent the number of cycles required for a sample to pass a fluorescence threshold. As the DNA is amplified additional fluorescent signal is produced, with each cycle resulting in an approximate doubling of the DNA. Therefore, higher levels of DNA (directly related to the amount of antigen in the sample) result in lower C t values.Calculate the mean C t for each set of triplicate standards, controls and samples. Subtract the C t value of each sample from the control to obtain the difference between the control and sample (Delta C t). Plot the values of the standards on a graph using a log scale for concentration on the x axis. This graph is the quickest way to visualize results, although not the most accurate. If this method is used the concentration of unknown samples can be estimated using a logarithmic line of best fit.The line of best fit will have an equation y = mln(x)+b, where y is the Delta C t value and x is the concentration. It may be helpful to use 5 significant figures for m and b to minimize rounding errors. To calculate the concentration of unknown sample this can be entered into Excel in the following format=EXP((y-b)/m))Where y is the Delta C t obtained during the assay, and b and m are obtained from the line of best fitAlternatively, for a more accurate representation linear regression may be used. Both the Delta C t and Concentration can be transformed using a log base of 10, plotted on a graph as described above, along with a line of best fit (using a linear model). The equation of this line may be used to calculate the antigen concentration of unknown samples. This is the method used for the analysis spreadsheet for IQELISA available online.A. TYPICAL DATAThese data are for demonstration only. A standard curve must be run with each assay.B. SENSITIVITY and RECOVERYThe minimum quantifiable dose of RANTES is typically 0.48 pg/ml, however levels as lower than 0.48 pg/ml may be detected outside of the quantification range.Serum spike tests show recovery is 97% with a range from 87% to 112%ntraplate CV is below 10% for all samples and Interplate CV is below 15%X. TROUBLESHOOTING GUIDEProblem Cause SolutionPoor standard curve Inaccurate pipettingImproper standard dilutionCheck pipettesBriefly centrifuge standards anddissolve the powder thoroughly bygently mixingLow signal Too brief incubation timesInadequate reagentvolumes or improperdilutionEnsure sufficient incubation time.Assay procedure step 2 may bedone overnightCheck pipettes and ensure correctpreparationLarge CV Uneven pipettingBubbles present in wellsCheck pipettesLightly tap or use pipette tip todislodge from bottom of wellHigh background Plate is insufficientlywashedContaminated washbufferImproper TmReview the manual for proper wash.If using a plate washer, ensure thatall ports are unobstructed.Make fresh wash bufferCheck run parameters and calibrateinstrumentLow sensitivity Improper storage of theIQELISA kitImproper TmStore your standard at <-20°C afterreconstitution, others at 4°C.Check run parameters and calibrateinstrumentThis product is for research use only.©2019 RayBiotech, Inc11。
捷尼科比亚 慢病毒表达包装试剂盒 使用手册说明书
Lenti-Pac ™ HIV Expression Packaging KitFor optimized production of recombinant lentivirusCat. No. HPK-LvTR-20 (20 transfections) Cat. No. HPK-LvTR-40 (40 transfections)User Manual Version VGeneCopoeia, Inc.9620 Medical Center Drive, #101 Rockville, MD 20850 USA301-762-0888 or 866-360-9531***********************© 2009 GeneCopoeia, Inc.G C n p o eiae e o TMExpressway to DiscoveryUSER MANUALLenti-Pac™ HIV Expression Packaging KitI. IntroductionII. Kit Contents and StorageIII. Additional Materials Required or RecommendedIV. Getting StartedV. Lentivirus ProductionVI. Lentivirus Titer Estimation by TransductionVII. Transduction of Target Cells with LentivirusesVIII. Limited Use License and WarrantyI. IntroductionGeneCopoeia has multiple sets of over 40,000 human and mouse ORF expression clones as well as multiple sets of small hairpin RNAi (shRNA) clones against genome-wide target genes from human, mouse, rat, and other mammals in HIV-based lentiviral vector systems. HIV- (human immunodeficiency virus) based vectors are currently the most popular lentiviral-based expression systems and are very effective at transducing genes into a wide variety of dividing and non-dividing mammalian cells, both in vitro and in vivo. The lentiviral expression vectors can integrate into the genome of the target cells, resulting in the stable expression of transgenes. The GeneCopoeia third generation HIV-based lentivector systems meet Biosafety Level 2 (BSL-2) requirements based on the criteria published by the Centers for Disease Control.The GeneCopoeia Lenti-Pac™ HIV Expression Packaging System includes an optimized lentiviral packaging plasmid mix, an eGFP positive control plasmid, a new transfection reagent, EndoFectin™optimized for virus production and TiterBoost™ reagent that further increases the titers 5-10 fold. When combined with GeneCopoeia HIV-based lentiviral constructs the results are high titers and robust expression levels. The Lenti-Pac HIV Expression Packaging System safely ensures efficient expression of recombinant transcripts in mammalian cells.A dvantages of OmicsLink™ Lentiviral ORF Expression Clones and shRNA Clones∙High efficiency of gene delivery to virtually all mammalian cell types in vitro as well in vivo∙High expression levels of delivered genes (ORF expression clones)∙High knockdown efficiency against target mRNA transcripts (shRNA clones)∙Self-inactivation and no unwanted viral replicationII. Contents and Shipping/ StorageContents and storage recommendations for the Lenti-Pac HIV Expression Packaging Kits(Cat. No. HPK-LvTR-20/HPK-LvTR-40) are provided in the following table.III. Additional Materials Required or Recommended1. GeneCopoeia GCI-L3 chemically competent cells (GeneCopoeia Cat No. STK300-10). Alternatively, Stbl3™chemically competent cells (Invitrogen Cat No. C7373-03) may be used.2. GeneCopoeia 293Ta Lentiviral packaging cell line(GeneCopoeia Cat No. Clv-PK-01). Alternatively, theHEK 293T/17 cell line (ATCC Cat No. CRL-11268) can also be used.3. H1299 cell line (ATCC Cat No.CRL-5803), or HT-1080 cell line (ATCC Cat No. CCL-121) for lentivirus titerestimation. H1299 cells are preferred over HT-1080 cells for lentivirus titration.4. DMEM with glucose, L-glutamine and sodium pyruvate (Mediatech Cat No. 10-013-CV)5. Fetal bovine serum (Thermo Scientific Cat No. SH300700.02)6. Opti-MEM® I Reduced-Serum Medium (Invitrogen Cat No. 31985-062/31985-070).7. Polybrene(Sigma-Aldrich Cat No. H9268): 10 mg/ml solution dissolved in 150 mM NaCl and sterile-filtered.Store usable aliquots at -20o C. Working stock can be stored at 4o C for up to two months.8. Crystal Violet (Sigma-Aldrich Cat No. C3886): 0.5% (W/V) solution dissolved in 25% methanol.9. Penicillin-Streptomycin for mammalian cell culture (Sigma-Aldrich Cat No. P4333)10. Antibiotics for selecting stably transduced cells: puromycin (Invivogen Cat No. ant-pr-1/ant-pr-5), hygromycinB (Invivogen Cat No. ant-hm-1/ant-hm-5), Neomycin (G-418) (Invivogen Cat No. ant-gn-1/ant-gn-5)11. BD Falcon® 5-ml or 14-ml Tubes (BD Falcon Cat No. 352053/352059).IV. Getting StartedUpon receipt of a new lentiviral expression plasmid, it is recommended to transform the plasmid into GeneCopoeia GCI-L3 Chemically Competent E. coli Cells (GeneCopoeia Cat No. STK300-10), or any Stbl3™-equivalent competent cell strain, and to prepare plasmid DNA with a proven plasmid DNA purification method. GeneCopoeia lentiviral ORF expression or shRNA constructs contain the ampicillin resistance gene.Quality of plasmidIt is critical to use plasmid of the highest quality. Determine the DNA concentration by reading the absorption at 260 nm. DNA purity is measured by using the 260 nm / 280 nm ratio (the ratio should be in the range of 1.8 to 2.0). Check the integrity of the plasmid by agarose gel electrophoresis.Condition of cellsAlways use healthy cells that are well maintained and passaged regularly. Make sure the culture is free from bacteria, fungi, or Mycoplasma contamination. If the cells are from a recent liquid nitrogen stock, passage the cells at least 2 times before transfection.V. Lentivirus ProductionThe GeneCopoeia HIV-Based Lentiviral Expression System is a modified version of the third generation self-inactivating (SIN) lentiviral vector system which incorporates enhanced bio-safety features and is optimized for production of high viral titers. In this system, recombinant lentiviral particles are generated by co-transfecting an HIV-based lentiviral expression plasmid together with the GeneCopoeia Lenti-Pac HIV Expression Packaging Kit into GeneCopoeia 293Ta lentiviral packaging cells (GeneCopoeia Cat No. CLv-PK-01).The lentiviral ORF/shRNA expression plasmid (lentiviral transfer vector) contains the elements required for packaging, transduction and stable integration of the viral expression construct into genomic DNA leading to expression of the ORF or shRNA hairpin. However, it lacks the elements essential for transcription and packaging of an RNA copy of the ORF/shRNA expression plasmid into recombinant pseudoviral particles. These elements are provided by the Lenti-Pac HIV packaging mix, an optimized mixture of plasmids that express the structural, regulatory, and replication genes required to produce lentivirus. See figure 1 below for schematics of this process. The lentivirus generated with this system is pseudotyped with vesicular stomatitis virus-G protein (VSV-G) which exhibits wide cell tropism and generates high titers.In addition to Lenti-Pac HIV packaging mix, this kit also includes a positive control lentiviral transfer vector that expresses the eGFP protein, EndoFectin Lenti Reagent (Cat No. EFL1001-01), and TiterBoost reagent. The EndoFectin Lenti transfection reagent is optimized for transferring plasmids into packaging cells. It guarantees higher transfection efficiency and lower cell toxicity compared to other commercially available transfection reagents. The unique TiterBoost reagent from GeneCopoeia enhances the production of lentivirus particles.Figure 1. Schematics of Lentivirus production and infection of target cellsThe following procedure provides optimized steps for lentivirus production in 293Ta packaging cells. The yield of recombinant lentiviral particles typically produced under these optimized conditions is 10 ml of 1–10 x107 infection units (ifu) per ml of un-concentrated supernatant from one 10-cm culture dish for eGFP or mCherry positive controls. This amount of pseudoviral particles is generally sufficient to infect 1–10 x108 target cells at a MOI (multiplicity of infection) equal to 1. The titers of lentivirus decrease as the size of insert increases. Actual lentivirus titers for your gene of interest will vary accordingly.Caution: Following this protocol results in the production of pseudoviral particles capable of infecting mammalian cells. The recommended guidelines for working with BSL-2 safety class must be adhered to.1. Plate packaging cellsTwo days before transfection, plate 1.3–1.5 x106of the GeneCopoeia 293Ta lentiviral packaging cells or comparable cells in a 10-cm dish in 10 ml of DMEM supplemented with 10% heat-inactivated fetal bovine serum so that the cells are 70–80% confluent at the moment of transfection. Incubate the cells at 37°C with 5% CO2.Note: Plating the packaging cells 2 days prior to transfection significantly increases the titer of lentivirus.Use heat-inactivated fetal bovine serum for lentivirus production. Heat-inactivated serum can be purchased from other vendors or prepared by incubating thawed serum for 30 minutes at 56°C with gentle shaking.2. Prepare DNA/EndoFectin Lenti complexIn a sterile polypropylene tube, dilute 2.5 µg of lentiviral ORF/shRNA expression plasmid and 5.0 µl (0.5 µg/µl) of Lenti-Pac HIV mix into 200 µl of Opti-MEM® I (Invitrogen). In a separate tube, dilute 15 µl of EndoFectin Lenti into 200 µl of Opti-MEM I. Add diluted EndoFectin Lenti reagent drop-wise to the DNA solution while gently vortexing the DNA-containing tube. Do not reverse the addition sequence. Use round-bottom polypropylene tubes such as Falcon® 5-ml or 14-ml tubes (BD) for larger volumes. Incubate the mixture for 10–25 minutes atroom temperature to allow the DNA-EndoFectin complex to form.Note: The DNA-EndoFectin complex must be formed in the absence of proteins even though the complex is able to transfect cells in the presence of proteins such as 10% serum. Opti-MEM I is recommended for diluting both DNA and EndoFectin Lenti reagent. Serum-free DMEM can be used in place of Opti-MEM I but the transfection efficiency will be compromised. The ratio of 3.0 µl of EndoFectin Lenti per 1 µg of plasmid has been found to be optimal. Increasing the ratio does not further improve transfection efficiency.3. Transfect packaging cellsAdd the DNA-EndoFectin Lenti complex directly to each dish and gently swirl the dish to distribute the complex.Incubate the cells in a CO2 incubator at 37°C overnight (8–14 hours). Replace the overnight culture medium with fresh DMEM medium supplemented with 2–5% heat-inactivated fetal bovine serum and penicillin-streptomycin. Add 1/500 volume of the TiterBoost reagent to the culture medium and continue incubation in the CO2 incubator at 37°C.Note:a. Replace the culture medium that contains the DNA-EndoFectin Lenti complex within 16 hours post-transfection.b. TiterBoost reagent at working concentration (1×) typically boosts the titer of lentivirus products 5-10 fold.This reagent is readily removed during commonly used lentivirus concentration/purification procedures such as ultracentrifugation, ultrafiltration and other chromatographic methods. If crude lentiviral particles are used directly to transduce target cells, the volume of lentiviral particles should not exceed 1/10 of that of culture medium; otherwise some adverse effects may occur. TiterBoost at 1/20× or lower concentrations has negligible effects on commonly used mammalian cell lines. It's advised to test the effects of TiterBoost on your target cells beforehand if large volumes of crude lentiviral particles are used.4. Harvest lentivirusCollect the pseudovirus-containing culture medium in sterile capped tubes 48 hours post transfection and centrifuge the tubes at 500 x g for 10 minutes to get rid of cell debris. Following centrifugation, filter the supernatant through 0.45 µm polyethersulfone (PES) low protein-binding filters.Note:a. Peak virus production is normally achieved 24–48 hours post transfection. Alternatively, lentivirus-containing medium may be collected multiple times at 36, 48 and 60 hours post-transfection. Lentiviral containing supernatants should be replaced with fresh DMEM supplemented with 2–5% heat-inactivated fetal bovine serum, penicillin-streptomycin and TiterBoost reagent.b. Do not use nitrocellulose filters as nitrocellulose is known to bind lentivirus and reduce titers.The supernatant containing lentiviral particles can be used directly to determine the titer and to transduce target cells in vitro as long as the target cells can survive in conditioned medium. Lentiviral stocks should be aliquoted and stored at -80°C. Expect significant loss of viral titer with each freeze/thaw cycle.VI. Lentivirus Titer Estimation by TransductionAt this point it is recommended that the pseudoviral stock is titered to ensure it is viable and to test what fraction of target cells can be transduced. This enables the number of copies of viral construct per target cell to be controlled. There are several methods to determine the titer of pseudovirus stock. The procedure below is based on the transduction of H1299 or HT-1080 cells. Different cells can also be used but titers may vary up to several orders of magnitude.Day 1: Plate H1299 or HT-1080 cells1. Plate ~5 x 104 of the H1299 or HT-1080 cells per well in a 24-well plate 24 hours prior to viral infection. Use 0.5 ml of DMEM supplemented with 10% heat-inactivated fetal bovine serum and penicillin-streptomycin for each well. Incubate the cells at 37°C with 5% CO2 overnight.Day 2: Transduce H1299 or HT-1080 cells2. Dilute Polybrene to 10 µg/ml with DMEM containing 5% heat-inactivated fetal bovine serum and penicillin-streptomycin.3. Remove old culture medium from each well. Add 0.25 ml of Polybrene (from Step 2). The final concentration of Polybrene will be 5 µg/ml after adding diluted lentivirus. For each pseudoviral stock, use five wells.3a. For lentivirus containing a fluorescent marker and to be analyzed with flow cytometry (step 5a): Infect H1299 or HT-1080 cells by adding 0.1 μl of lentivirus (10 μl of 100-fold diluted viral stock) into the first well, 0.5 μl of lentivirus (50 μl of 100-fold diluted viral stock) into the second well, 2.0 μl into the third well, 10 μl into the fourth well, and 50 μl into the fifth well. Add appropriate amount of DMEM containing 5% heat-inactivated serum and penicillin-streptomycin so that the final volume reaches 0.5 ml per well.3b. For lentivirus to be analyzed by drug selection and colony counting (step 5b-10): Infect H1299 or HT-1080 cells by adding 0.001 μl of lentivirus (10 μl of 10,000-fold diluted viral stock) into the first well, 0.01 μl of lentivirus (10 μl of 1,000-fold diluted viral stock) into the second well, 0.1 μl of lentivirus (10 μl of 100-fold diluted viral stock) into the third well, 0.5 μl of lentivirus (50 μl of 100-fold diluted viral stock) into the fourth well, and 2.0 μl into the fifth well. Add appropriate amount of DMEM containing 5% heat-inactivated serum and penicillin-streptomycin so that the final volume reaches 0.5 ml per well.Place the plates for 2 hours at 4-8°C; then transfer the plates to a 37°C incubator with 5% CO2 and incubate cells overnight.Note:a. Dilute lentivirus with only culture medium. Do not use water or other buffers.b. The optimal concentration of Polybrene depends on cell type and may need to be empirically determined,but is usually in the range of 2–10 µg/ml. Prepare enough for an extra well as a negative control.c. Excessive exposure to Polybrene (>12 hr) can be toxic to some cells.Day 3: Replace medium/split cell culture4. Trysinize and transfer the cells to 6-well plates (each 6-well plate will be used for one lentivirus with one control well of non-transduced cells). Incubate in DMEM supplemented with 10% fetal bovine serum and penicillin-streptomycin for additional 48 hours.Day 5: Determine titer by fluorescence analysis (step 5a) or drug selection (5b)5a. The fraction of eGFP fluorescent cells can be counted by FACS (fluorescent activated cell sorting). Alternatively the eGFP fluorescence may be visualized under a fluorescent microscope. Normally 10 random fields of view are used to estimate the overall fraction of fluorescing cells in each well. The cells are then trypsinized, suspended with complete DMEM, and the total number of cells in each well is determined by using a hemocytometer. The averaged fraction of fluorescent cells is multiplied by the corresponding total cell numbers, then divided by the actual volume of added lentivirus supernatant (in ml, e.g. 0.1 μl equals 10–4 ml) to determine the titer of the pseudovirus in the supernatant.5b. For HT-1080 or H1299 cells transduced with lentiviral stocks lacking a fluorescent marker, replace the old medium with fresh complete DMEM containing an appropriate selection drug. (Note: The concentration of the selection drug should be determined empirically beforehand.) Then follow steps 6–10 below:Days 6-14: Select stably transduced cells and count colonies (continued from step 5b)6. Replace medium with fresh complete medium containing the appropriate selection drug every 3–4 days until drug-resistant colonies become visible (generally 7–14 days after selection). There should not be any colonies in the mock well control.7. Remove medium and wash dishes twice with cold PBS.8. Add enough 10% formalin to cover each dish. Incubate for 5 minutes at room temperature to fix the cells.9. Decant 10% formalin, add 0.5% crystal violet and incubate for 10 minutes at room temperature.10. Remove the crystal violet solution and wash the dishes with tap water until there is no background staining. Count the blue colonies and calculate the titer of lentiviral stock.Note:Viral titers determined by drug selection/colony counting are significantly lower than those determined by FACS.VII. Transduction of Target Cells with LentivirusesThe transduction efficiency depends upon the target cells and experimental procedure. It is recommended that the titrated pseudoviral stock containing the positive control eGFP is used to determine the concentration of pseudoviral particles required for the desired MOI of the target cells. After these test transductions are performed, it should be possible to determine the optimum concentration of pseudoviral particles for transduction based on eGFP fluorescence.Day 1: Plate cells1. Plate 2–10 x 104 of the target cells per well in a 24-well plate 24 hours prior to viral infection. Use 0.5 ml of DMEM supplemented with 5% heat-inactivated serum and penicillin-streptomycin for each well. Incubate the cells at 37°C with 5% CO2 overnight.Day 2: Transduce target cells2. For each well, prepare 0.5 ml of virus suspension diluted in complete medium with Polybrene at a final concentration of 5–8 µg/ml.Note:Use several dilutions of pseudoviral stock (0.1 μl to 100 μl). In addition, we recommend including a transduction with the eGFP control and other appropriate positive and negative controls. Mix the virus with the medium gently by rotation or inversion. Do not vortex.3. Infect the target cells by removing the old culture medium and replacing it with 0.5 ml of diluted viral supernatant. For one well (mock well control), add 0.5 ml of complete DMEM with Polybrene. Place the plates in a 37°C incubator with 5% CO2 and incubate cells overnight. (Optional: Place the plates for 2 hours at 4-8°C; then transfer the plates to a 37°C incubator with 5% CO2 and incubate cells overnight.)Note:Incubating cells with lentivirus for 2 hours at low temperatures can significantly increase the transduction efficiency. But it may be omitted if the cells cannot tolerate low temperatures.Day 3: Replace medium/Split cell culture4. Remove the culture medium and replace with 0.5 ml of complete medium (without Polybrene). Or split the cells 1:3 to 1:5 depending on the type of cells, and continue incubating for 48 hours in DMEM.Day 5: Analyze transduced cells or start drug selection of stably transduced cells5a. The infected target cells can be analyzed for transient expression of transgenes using an appropriate biological assay. If you have used an internal eGFP control, determine the percentage of infected cells by counting fluorescing cells by flow cytometry or with a fluorescent microscope.5b. To select stably transduced cells, replace old medium with fresh complete medium containing the appropriate selection drug every 3–4 days until drug-resistant colonies become visible (generally 7–14 days after selection).VIII. Limited Use License and WarrantyLimited Use LicenseFollow ing terms and conditions apply to use of all OmicsLink™ ORF Expression Clones in all lentiviral vectors and Packaging Kit (the Product). If the terms and conditions are not acceptable, the Product in its entirety must be returned to GeneCopoeia within 5 calendar days. A limited End-User license is granted to the purchaser of the Product. The Product shall be used by the purchaser for internal research purposes only. The Product is expressly not designed, intended, or warranted for use in humans or for therapeutic or diagnostic use. The Product must not be resold, repackaged or modified for resale, or used to manufacture commercial products without prior written consent from GeneCopoeia. This Product should be used in accordance with the NIH guidelines developed for recombinant DNA and genetic research. Use of any part of the Product constitutes acceptance of the above terms.Limited WarrantyGeneCopoeia warrants that the Product meets the specifications described in the accompanying Product Datasheet. If it is proven to the satisfaction of GeneCopoeia that the Product fails to meet these specifications, GeneCopoeia will replace the Product. In the event a replacement cannot be provided, GeneCopoeia will provide the purchaser with a refund. This limited warranty shall not extend to anyone other than the original purchaser of the Product. Notice of nonconforming products must be made to GeneCopoeia within 30 days of receipt of the Product. GeneCopoe ia’s liability is expressly limited to replacement of Product or a refund limited to the actual purchase price. GeneCopoeia’s liability does not extend to any damages arising from use or improper use of the Product, or losses associated with the use of additional materials or reagents. This limited warranty is the sole and exclusive warranty. GeneCopoeia does not provide any other warranties of any kind, expressed or implied, including the merchantability or fitness of the Product for a particular purpose.GeneCopoeia is committed to providing our customers with high-quality products. If you should have any questions or concerns about any GeneCopoeia products, please contact us at (301)-762-0888.© 2009, GeneCopoeia, Inc.GeneCopoeia, Inc.9620 Medical Center Drive, #101Rockville, Maryland 20850Tel: 301-762-0888 Fax: 301-762-8333Email:***********************Web: GeneCopoeia Products are for Research Use Only Copyright © 2009 GeneCopoeia, Inc.。
基尔斯特 5015a 电荷计用户手册说明书
Page 1/6Electronics & SoftwareCharge MeterUniversally Applicable for Piezoelectric Measuring Technology5015A _000-297e -01.21© 2010 ... 2021 Kistler Group, Eulachstrasse 22, 8408 Winterthur, Switzerland . Kistler Group products are This information corresponds to the current state of knowledge. Kistler reservesthe right to make technical changes. Liability for consequential damage resulting Type 5015A...This instrument can be used wherever mechanical quantities are measured with piezoelectric sensors. Piezoelectric sensors produce an electric charge which varies in direct proportion to the load acting on the sensor.• Single-channel charge amplifier • Piezotron input (option)• Measure-jump compensated• Liquid crystal display (128x128 pixels)• Menu-driven operation • Direct signal evaluation• Flexible adjustment of high-pass and low-pass filters • Compatible with Charge Amplifier Type 5011B...• PC-Software and Virtual Instrument Driver for LabVIEW DescriptionThe Type 5015A… is not only a charge amplifier but an uni-versal Charge Meter with a graphical liquid crystal display. However, the 19"-r ack module is also suitable for measurements in an industrial environment. It can display instantaneous, peak and average values as well as reference deviations. State-of-the art technology allows the naturally occurring interference to be almost entirely eliminated. The instrument is distinguished first-ly by its excellent technical data and secondly by its extremely simple operation.ApplicationThe instrument has been designed for use in research, devel-opment and the laboratory.OperationPage 2/65015A _000-297e -01.21© 2010 ... 2021 Kistler Group, Eulachstrasse 22, 8408 Winterthur, Switzerland Tel.+41522241111,****************,. Kistler Group products are This information corresponds to the current state of knowledge. Kistler reserves the right to make technical changes. Liability for consequential damage resulting Drift, meas. mode voltage DC (long) (@ Range 10 V FS; Gain = 1) at 25 °C, max. relative mV/s <±0,03 humidity RH of 60 % (non-condensing)at 50 °C, max. relative mV/s <±0,3 humidity RH of 50 % (non-condensing)Max. common mode voltage V <±30 between input and output ground Overload %F S ≈±105Piezotron mode Supply current mA 4 ±10 % Input voltage swing V 0 (20)Voltage Output Connector Type BNC neg.Output range FS V ±10/±5/±2,5/±2Output current mA <±2Output impedance Ω ≈10Measure-jump Compensated Measure-jump (Long) mV <±3 Correction time, inclusive reed-relay delay time ms <15 1)Zero errors mV <±2Output interference (0,1 Hz ... 1 MHz), Type 5015Axxx0 Range FS, LP filter off 2,000 ... 9,999 pC mVpp <140 ... <40 10,00 ... 99,99 pC mVpp <30 ... <10 1) 100,0 ... 999,9 pC mVpp <15 ... <7 1) ... mVpp <15 ... <7 1) 0,220 ... 2,200 µC mVpp <15 ... <7 1) Range FS, LP filter 30 kHz 2,000 ... 9,999 pC mVpp <60 ... <20 10,00 ... 99,99 pC mVpp <20 ... <7 1) ... mVpp <10 ... <5 1) 0,220 ... 2,200 µC mVpp <10 ... <5 1)Output interference (0,1 Hz ... 1 MHz), Type 5015Axxx1 Range FS, LP filter off 2,000 ... 9,999 pC, mV mVpp <220 ... <50 10,00 ... 99,99 pC, mV mVpp <50 ... <12 1) 100,0 ... 999,9 pC, mV mVpp <20 ... <7 1) ... mVpp <20 ... <7 1) 0,220 ... 2,200 µC mVpp <20 ... <7 1) Range FS, LP filter 30 kHz 2,000 ... 9,999 pC, mV mVpp <180 ... <50 1) 10,00 ... 99,99 pC, mV mVpp <30 ... <10 1) 100,0 ... 999,9 pC, mV mVpp <10 ... <5 1) ... mVpp <10 ... <5 1) 0,220 ... 2,200 µC mVpp <10 ... <5 1) 1)Values valid from MCC version V2.xxFrequency Response DC (Long), LP-filter off Bandwidth (–3 dB) kHz ≈0 ... 200 Group delay µs ≈10High-pass Filter (1st order)Analog high-pass filter DC (Long)Range FS Charge, (Voltage) 2 pC, (mV) s 10 000 1 000 pC, (mV) s 100 000Time constants Medium s 1/10/100/220 Short s 0,1/1/10/220Tolerance % <±20Digital high-pass filter computed by DSPTime constantsRange FS Charge, (Voltage) 2 pC, (mV) s 0,01/0,1/1 100 pC, (mV) s 0,01/0,1/1/10 ≥1 000 pC, (mV) s 0,01/0,1/1/10/100Tolerance % <±20Cutoff frequencies –3 dB Hz 16/1,6/0,16/0,016/ 0,0016 –10 % Hz 30/3/0,3/0,03/0,003 –5 % Hz 50/5/0,5/0,05/0,005 –1 % Hz 100/10/1/0,1/0,01Low-pass FilterDigital low-pass filter computed by DSP Filter Type IIR, linear phase Order 2. or 5.Cutoff frequency (–3 dB) Hz 5, 10, 20, 30, 50, 100, 200, 300, 500 kHz 1, 2, 3, 5, 10, 20, 22, 30, (LP off)Tolerance % <±10Page 3/65015A _000-297e -01.21© 2010 ... 2021 Kistler Group, Eulachstrasse 22, 8408 Winterthur, Switzerland Tel.+41522241111,****************,. Kistler Group products are This information corresponds to the current state of knowledge. Kistler reserves the right to make technical changes. Liability for consequential damage resulting Signal Evaluation Sample rates LP-filter on ksps 400 LP-filter offksps 1 000Minimum pulse width for peak-peak value detection LP-filter 5 Hz ... 30 Hz µs >2 500 LP-filter 50 Hz ... 300 Hz µs >250 LP-filter 500 Hz ... 3 kHz µs >25 LP-filter 5 kHz ... 30 kHz µs >2,5 LP-filter offµs >1Max. integration time for mean value min <75Integration time for the updating rate of the liquid crystal display Instant value ms 300 Characteristic values ms 300 Bar graph ms 17,5Remote Control Connector Type MiniDin round socked Pin allocationInputs with internal pull-up resistor Pin 4 (input) Window (remote) Pin 5 (input) Measure (remote) Pin 6 DGND Input voltagelogic inactive or input open V 3,5 ... 30 logic active V(mA) 0 ... 1 (0 ... 4)Delay timeWindow (remote) ms <0,5 Measure (remote) ms <15Digital Measuring Data TransferThe instrument provides a continuos measuring data transfer via the serial interface to a PC. For this the PC software (Windows) of the VI driver (LabVIEW) is required. This feature is not available on the IEEE-488 interface. Sampling rates ksps0,1/0,25/0,5RS-232C Interface (Electrically Separated)EIA-standard RS-232C Connector Type DB-9S (D-Sub)Pin allocation Pin 2 RxD Pin 3 TxD Pin 5 SG Max. cable length at 9 600 bps m <15 19 200 bps m <15 38 400 bps m <12 57 600 bps m <10 115 200 bps m <5Max. input voltage,continues V <±20Max. voltage between signal ground and protective ground V RMS <20Baud rates bps 1 200/9 600/ 19 200/38 400/ 57 600/115 200Data-bit 8Stop-bit 1Parity none SW handshake noneIEEE-488 Interface (Option)Standard IEEE-488.1-1987 Connector Type Microribbon series 57 (24-pole)Max. distance between devices m 2Max. bus length m 20Max. number of devices 15Adress range 0 ... 30Functions Listener and Talker Interface functions SH1, AH1, L4, LE0, T6, TE0, SR1, RL2, PP0,DC1, DT1, C0, E1Multiline commands DCL, SDC, GET, UNL, UNT, SPE, SPD Uniline commands IFC, REN, EOI, SRQ, ATNPage 4/65015A _000-297e -01.21© 2010 ... 2021 Kistler Group, Eulachstrasse 22, 8408 Winterthur, Switzerland Tel.+41522241111,****************,. Kistler Group products are This information corresponds to the current state of knowledge. Kistler reserves the right to make technical changes. Liability for consequential damage resultingPower Supply ConnectionPower plug (2P+E, protect. class I) IEC 320C14Supply voltage setable V~ 115/230Supply voltage tolerance % –22, +15Supply frequency Hz 48 ... 62Consumption VA ≈20Voltage between Signal ground and protective ground V RMS <50FusesF1 (slow) mA 100 F2 (slow) mA 100Remaining DataIP-Degree of protection IP40, IEC 60529Operating temperature °C 0 ... 50Storage temperature °C –10 (70)Rel. humidity, not condensing %10 (80)Vibration steadiness(20 Hz ... 2 kHz, duration 16 min, cycle 2 min.) g <10Shock steadiness (1 ms) g <200Housing dimensions with frame (wxhxd) mm 105,3x142x253,15 without frame (wxhxd) mm71,12x128,7x230Front panel (accordingDIN 41494, part 5/IEC 60297) HE/TE 3/14Weightkg ≈2,3Fig. 1: Block Diagram of charge meter Type 5015A…Page 5/65015A _000-297e -01.21© 2010 ... 2021 Kistler Group, Eulachstrasse 22, 8408 Winterthur, Switzerland Tel.+41522241111,****************,. Kistler Group products are This information corresponds to the current state of knowledge. Kistler reserves the right to make technical changes. Liability for consequential damage resultingFig. 2: Desktop Type 5015A1… (stackable)Fig. 3:19"-Rack plug-in Type 5015A0…Page 6/65015A _000-297e -01.21© 2010 ... 2021 Kistler Group, Eulachstrasse 22, 8408 Winterthur, Switzerland Tel.+41522241111,****************,. Kistler Group products are This information corresponds to the current state of knowledge. Kistler reserves the right to make technical changes. Liability for consequential damage resulting Included AccessoriesCharge Meter Type 5015A... with • Country-specific power cord • Plug for 'Remote Control'• Self-adhesive label with supply voltage details • Flash-Loader with current firmware• Demo-Program for visualization of the display on a PC • PC-Software and VI-Driver for LabVIEW for the equip-ment configuration and measured data acquisition • Instruction manual • Calibration sheetOptional Accessories Type/Art. No.• RS-232C cable, l = 5 m, null-modem, 1200A27 DB-9P/DB-9S• or PC-link cable RS-232C, l = 3 m, 1465A3 DB-25P/DB-9S• with suitable D-Sub adapter , 1479 DB-9P/DB-25SInstrument ConfigurationsThe complete type designation of the Charge Meter is made up of the basic type designation Type 5015A... and four ad-ditional digits.The basic type contains a single-channel Charge Meter (with charge input for piezoelectric sensors) with display unit and RS-232C interface in the following versions:Ordering Key Type 5015ASize/Measuring Range19" rack module version according to 0 DIN 41494; width 14 TE and height 3 HE Desktop version with support bracket 1Without interface option0With IEEE-488 interface (option) 1Adjusted in the factory to 230 V~; 0switching to 115 V~supply by the user possible at any time Adjusted in the factory to 115 V~; 1switching to 115 V~supply by the user possible at any time Without voltage input0With voltage input for sensors with 1integrated Piezotron circuitry (option)Windows is a registered trademark of Microsoft bVIEW is a registered trademark of National Instruments Corporation.。
艾法迪产品介绍0422
请按照标签推荐用药， 选择合适的施药时间， 减少抗药性或耐药性 害虫的扩散
艾法迪与现有所有杀虫剂无交互抗性
氨基甲酸酯类－西维因、灭多威、拉维因、呋喃丹 有机磷类－甲胺磷、乙酰甲胺磷、谷硫磷 拟除虫菊酯类－顺式氰戊菊酯、三氟氯氰菊酯、溴氰菊酯
氯离子通道激活剂－阿维菌素、甲维盐
苯甲酰脲类－除虫脲、灭幼脲、氟铃脲 茚虫威－安打
是抗性治理的绝佳选择
交替使用可以延缓对所有杀虫剂的抗性发展 替代代谢抗性已经产生的产品
持效活性
全新作用机制
无需代谢激活
通过胃毒及有限的触杀活性来杀虫
无交互抗性
对抗性害虫也有效
高效杀虫
对各龄期的靶标害虫幼虫均有效
对作物高度安全
在植物体内没有内吸传导性
产品简介 作用机制 适用作物及害虫防治 安全性 抗性管理 产品总结
适用作物
中国登记情况 全球登记情况 叶菜类 已登记 水稻 登记中
杀虫活性
对咀嚼式口器的昆虫种类有明显杀虫效果
对半翅目盲蝽类害虫有很好的防治效果
可用于防治蚂蚁、白蚁、 红火蚁、蝇及蟑螂
产品简介 作用机制 适用作物及害虫防治 安全性 抗性管理 产品总结
作物的安全性
良好的作物安全性
如菜心、菜花、花椰菜、白菜、油菜、芥菜、 莴苣、茄子、 西红柿、辣椒、甜辣、马铃薯、 韭菜、胡萝卜、 草莓、西瓜、豆类、朝鲜蓟、 棉花、甜菜、大麦、水稻、苹果、葡萄、橄榄、柑桔等作物。
对有益节肢动物的影响
小花蝽 、大眼长蝽、姬蝽 在紫花苜蓿上试验2次
平均益虫数
200g有效成分/公顷
有效成分/公顷
34g有效成分/公顷
田间试验表明艾法迪对对紫花苜蓿上的天敌影响较小
PulsaFeeder
Suction and discharge tubing: 1/4” I.D. x 3/8”O.D.
Injection/check valve assy: 1/2” MNPT connection
Power requirements: 115 VAC, 50/60 Hz, 1 phase
ETL, ETL-Canada, ETL-Sanitation, CE
Table 1 – Nalco Pump Part Numbers
Nalco Part Number 121-PJ3010.88 121-PJ3011.88 121-PJ3012.88 121-PJ3013.88 121-PJ3014.88 121-PJ3015.88
• High-quality solenoid and heat dissipating
enclosure ensure reliable performance even with continuous duty operation
• Straight flow path and ample clearance between the
Liquid End Materials
Capacity Max. (gpd/gph) psi
PP/PTFE/CE 12 / 0.5
250
PVDF/PTFE/CE 12 /E 33 / 1.38 150
PVDF/PTFE/CE 33 / 1.38 150
PP/PTFE/CE 58 / 2.42 100
Specifications
Delivery rate: See Table 1 Maximum pressure limits: See Table 1
Fluke 902 HVAC Clamp Meter Users Manual
®PN 2547887 May 2006 Rev. 1, 3/07© 2006-2007 Fluke Corporation. All rights reserved. Printed in China.All product names are trademarks of their respective companies.902HVAC Clamp MeterUsers ManualLIMITED WARRANTY AND LIMITATION OF LIABILITY This Fluke product will be free from defects in material and workmanship for three years from the date of purchase. This warranty does not cover fuses, disposable batteries, or damage from accident, neglect, misuse, alteration, con-tamination, or abnormal conditions of operation or handling. Resellers are not authorized to extend any other warranty on Fluke’s behalf. To obtain service during the warranty period, contact your nearest Fluke authorized service cen-ter to obtain return authorization information, then send the product to that Service Center with a description of the problem.THIS WARRANTY IS YOUR ONLY REMEDY. NO OTHER WARRANTIES, SUCH AS FITNESS FOR A PARTICULAR PURPOSE, ARE EXPRESSED OR IMPLIED. FLUKE IS NOT LIABLE FOR ANY SPECIAL, INDIRECT, INCIDEN-TAL OR CONSEQUENTIAL DAMAGES OR LOSSES, ARISING FROM ANY CAUSE OR THEORY. Since some states or countries do not allow the exclusion or limitation of an implied warranty or of incidental or consequential dam-ages, this limitation of liability may not apply to you.Fluke CorporationP.O. Box 9090 Everett, WA 98206-9090 U.S.A. Fluke Europe B.V. P.O. Box 1186 5602 BD Eindhoven The Netherlands11/99Table of ContentsTitle Page Introduction (1)Contacting Fluke (2)Safety Information (3)Symbols (5)Getting Acquainted with the Meter (6)Using the Meter (10)AC and DC Voltage Measurement (10)Resistance and Continuity (11)Microamps µA Measurement (12)Temperature (13)Capacitance (16)AC Current Measurement (16)Backlight (18)MIN MAX Recording Mode (18)Display HOLD (19)Auto Off (19)Maintenance (20)Cleaning the Meter (20)Battery Replacement (21)Specifications (23)Electrical Specifications (23)General Specifications (24)902Users ManualList of TablesTable Title Page1. 902 HVAC Clamp Meter Features (7)2. Display Features (9)List of FiguresFigure Title Page1. 902 HVAC Clamp Meter Features (6)2. Display Features (8)3. Testing a Flame Rod (13)4. Temperature Measurement (15)5. Proper AC Current Measurement (17)6. Battery Replacement (22)902Users Manual902 IntroductionThe Fluke 902 is a hand-held battery-operated HVAC Clamp Meter (“the Meter”) that measures:•AC current•DC current (up to 200 µA for flame rod testing)•AC and DC voltages•Capacitance•Resistance•Continuity•Temperature in both Celsius (°C) and Fahrenheit (°F) The Meter comes with:•Two AA alkaline batteries (installed)•Users Manual•Soft carrying case•TL75 Test Leads (one pair)•80BK Integrated DMM Temperature Probe902Users ManualContacting FlukeTo contact Fluke, call one of the following telephone numbers:USA: 1-888-99-FLUKE (1-888-993-5853)Canada: 1-800-36-FLUKE (1-800-363-5853)Europe: +31 402-675-200Japan: +81-3-3434-0181Singapore: +65-738-5655Anywhere in the world: +1-425-446-5500Or visit Fluke’s Web site at: . Register the Meter at: HVAC Clamp MeterFlukeContacting Safety InformationA “XW Warning” statement defines hazardous conditions and actions that could cause bodily harm or death.A “W Caution” statement identifies conditions and actionsthat could damage the Meter or the equipment under test.XW Read First: Safety InformationTo ensure safe operation and service of theMeter, follow these instructions:•Read the Users Manual before use andfollow all safety instructions.•Use the Meter only as specified in theUsers Manual; otherwise, the Meter'ssafety features may be impaired.•Avoid working alone so assistance can berendered.•Never use the Meter on a circuit withvoltages higher than 600 V or a frequencyhigher than 400 Hz fundamental. The Metermay be damaged.•Never measure ac current while the testleads are inserted into the input jacks.•Do not use the Meter or test leads if theylook damaged.•Use extreme caution when working aroundbare conductors or bus bars. Contact withthe conductor could result in electricshock.902Users Manual•Use caution when working with voltages above 60 V dc or 30 V ac rms or 42 V acpeak. Such voltages pose a shock hazard.•Clean the case with a damp cloth and mild detergent only. Do not use abrasives orsolvents.•To avoid false readings that can lead to electrical shock and injury, replace thebatteries as soon as the low batteryindicator (B)appears. As the Meter getsto the point where the low batteries affectthe readings, the Meter locks and nomeasurements can be made until thebatteries are changed.•Do not hold the Meter anywhere beyond the tactile barrier, see Figure 1.•Adhere to local and national safety codes.Individual protective equipment must beused to prevent shock and arc blast injurywhere hazardous live conductors areexposed.HVAC Clamp MeterSymbolsSymbolsThe following symbols are found on the Meter or in this manual.,May be used on hazardous live conductorsW Risk of danger. Important information. See UsersManual.X Hazardous voltage. Risk of electric shock.T Double insulationB Battery)Complies with Canadian and US StandardsP Conforms to relevant European Union directivesJ Earth groundF DC (Direct Current)B AC (Alternating Current)~Do not dispose of this product as unsorted municipalwaste. Contact Fluke or a qualified recycler fordisposal.;Conforms to relevant Australian standardsN10140s Inspected and licensed by TÜV Product Services902Users ManualGetting Acquainted with the Meter Refer to Figures 1 and 2 and Tables 1 and 2 to become more acquainted with the Meter’s features.Figure 1. 902 HVAC Clamp Meter FeaturesHVAC Clamp MeterGetting Acquainted with the Meter Table 1. 902 HVAC Clamp Meter FeaturesNumber DescriptionA Backlight ButtonB Jaw ReleaseC Tactile BarrierD JawsE Hold ButtonF Rotary Switch:V DC and AC voltageP Resistance and continuityG DC microampsF Degrees Fahrenheit / degrees CelsiusL CapacitanceK AC currentG LCDH Min Max ButtonI AC/DC, °F/°C ButtonJ Input Terminals902Users ManualFigure 2. Display FeaturesHVAC Clamp MeterGetting Acquainted with the MeterTable 2. Display FeaturesNumber IndicationA Battery indicator -The batteries are low and need to be changed. XW Warning: To avoid false readings, which could lead to possible electric shock or personal injury, replace the batteries as soon as the battery indicator appears.B Indicates the presence of high voltageC Indicators for minimum and maximum recording modeD Display Hold is activeE VoltsF AmpsG °F - Degrees Fahrenheit °C - Degrees Celsius e - OhmsM - MicroampsN - MicrofaradsDC - Direct CurrentAC - Alternating Current902Users ManualUsing the MeterAC and DC Voltage MeasurementTo measure AC or DC voltage:1.Insert the test leads into the Meter.2.Turn the rotary switch to V.3.Press A to choose AC or DC voltage. The displayreflects the chosen voltage mode.e the test leads to take the measurement. The Meterreading appears on the display.NoteWhen a measured voltage is above 30 V,Z appears on the display. When the voltage dropsbelow 30 V, Z disappears.HVAC Clamp MeterUsing the MeterResistance and ContinuityTo measure resistance or continuity:XW WarningTo avoid false readings that can lead toelectrical shock and injury, de-energize thecircuit before taking the measurement.1.Insert the test leads into the Meter.2.Turn the rotary switch to P.3.Take the measurement. The resistance readingappears on the display.•If the resistance is shorted, the Meter beeps and shows a reading < 30 Ω.•If the resistance is open or exceeds the Meter’s range, the display reads OL.902Users ManualMicroamps µA MeasurementThe µA dc (G) function on the Meter is primarily for HVAC flame rod testing. To test a heating system flame rod (refer to Figure 3):1.Turn the heating unit off and locate the wire betweenthe gas-burner controller and the flame rod.2.Break this connection.3. Turn the rotary switch on the Meter to G.ing alligator clips, connect test leads between theflame sensor probe and control-module wire.5.Turn heating unit on and check the reading on theMeter.6.Refer to the heating unit documentation for what thedesired reading should be.HVAC Clamp MeterUsing the MeterFigure 3. Testing a Flame RodTemperatureThe Meter measures temperature in either Celsius (°C) or Fahrenheit (°F).To measure temperature (refer to Figure 4):902Users Manual1.Connect the 80BK Integrated DMM TemperatureProbe to the input jacks noting correct polarity of theprobe.2.Turn the rotary switch to F.3.Press A to select °C or °F. The display reflects thechosen temperature mode.4.Position the probe to take the measurement. Thereading appears on the display.NoteTo meet stated accuracy, the 80BK and Metermust be at the same temperature.XW WarningTo avoid possible electric shock DO NOT applythe probe tip to any conductor that is greaterthan 30 V ac, 42 V peak or 60 V dc to earth.Using the MeterFigure 4. Temperature MeasurementUsers ManualCapacitanceTurn off circuit power, then disconnect and discharge the capacitor before measuring capacitance. Turn the Meter’s rotary switch to capacitance (L).If the capacitor requires more discharging, diSC is displayed while the capacitor discharges. When measuring, be sure to note the correct polarity of the capacitor.AC Current MeasurementXW WarningTo avoid electrical shock and injury:•Remove Test Leads before making current measurements.•Do not hold the Meter anywhere beyondthe tactile barrier, see Figure 1.Turn the rotary switch to AC current (K). When measuring AC current, it is necessary that the measured wire be properly seated within the clamp jaws. The wire being measured should be centered within the jaws, below the horizontal line located on the clamp. Also note that currents moving in different directions will cancel each other out, so one wire must be measured at a time for a correct measurement (see Figure 5).Using the MeterFigure 5. Proper AC Current MeasurementUsers ManualBacklightPress C to toggle the backlight on and off. The backlight automatically turns off after 2 minutes.To disable the automatic 2-minute backlight timeout, hold down C while turning the Meter on.MIN MAX Recording ModeThe MIN MAX recording mode captures the minimum and maximum input values. When a new high or low is detected, the Meter beeps.To use this feature:1.Put the Meter into the desired measurement functionand range.2.Press J to enter MIN MAX Mode. MAX is displayedand the highest reading detected since entering MINMAX is displayed.3.Press J to step through the minimum (MIN) andpresent readings.4.To pause MIN MAX recording without erasing storedvalues, press I. K is displayed.5.To resume MIN MAX recording, press I again.6.To exit and erase stored readings, press J for atleast two seconds.Using the MeterDisplay HOLDXW WarningTo avoid possible electric shock or personalinjury, when Display HOLD is activated, beaware that the display will not change whenyou apply a different voltage.In the Display HOLD mode, the Meter freezes the display. The Meter also beeps every 4 seconds and H flashes to remind the user.Press I to activate Display HOLD; H is displayed and the reading is captured.To exit and return to normal operation, press I.Auto OffThe Meter automatically turns off after 20 minutes. The rotary switch must be turned to “OFF” and then turned back on for the Meter to restart. Auto Off is disabled during Min Max mode. To disable Auto Off, hold J when turning the Meter on.Users ManualMaintenanceXW WarningTo avoid possible electric shock or personalinjury, repairs or servicing not covered in thismanual should be performed only by qualifiedpersonnel.Cleaning the MeterXW WarningTo avoid electrical shock, remove any inputsignals before cleaning.W CautionTo avoid damaging the Meter, do not usearomatic hydrocarbons or chlorinated solvents for cleaning. These solutions will react with the plastics used in the Meter.Clean the instrument case with a damp cloth and mild detergent.MaintenanceBattery ReplacementXW WarningTo avoid false readings that could lead topossible electric shock or personal injury,replace the batteries as soon as the lowbattery indicator (B) appears.Disconnect the test leads before replacing thebatteries.To replace the batteries (refer to Figure 6):1.Turn the rotary switch to “OFF” and remove the testleads from the terminals.e a Phillips screwdriver to loosen the batterycompartment door screw, and remove the door fromthe case bottom.3.Remove the batteries.4.Replace the batteries with two new AA batteries.5.Reattach the battery compartment door to the casebottom and tighten the screw.Users ManualFigure 6. Battery ReplacementSpecifications SpecificationsElectrical SpecificationsFunction Range Resolution Accuracy Voltage DC 0 – 600 V 0.1 V 1 % ± 5 countsVoltage AC (True Rms) 0 – 600 V 0.1 V1 % ± 5 counts(50/60 Hz)Current AC (True Rms) 0 – 600 A 0.1 A2.0 % ± 5 counts(50/60 Hz)Current DC 0 - 200 µA 0.1µA 1.0 % ± 5 countsResistance 0 – 999 Ω0 – 9999 Ω0.1 Ω1.0 Ω1.5 % ± 5 countsContinuity <30ΩTemperature -10 to 400 °C 0.1 °C 1 % ± 0.8 °CCapacitance 1-100 µF100-1000 µF0.1 µF1 µF1.9 % ± 2 countsUsers ManualGeneral SpecificationsOperating Temperature -10 °C to +50 °CStorage Temperature -40 °C to +60 °COperating Humidity Non condensing (< 10 °C)90 % RH (10 °C to 30 °C)75 % RH (30 °C to 40 °C)45 % RH (40 °C to 50 °C)(Without Condensation) Operating Altitude 2500 meters above mean sealevelStorage Altitude 12,000 meters above meansea levelIP Rating IP 30 per IEC 60529 Vibration Requirements MIL-PRF-28800F Class 2random vibrationEMI, RFI, EMC EMI: instrument unspecified foruse in EMC field • 0.5 V /MeterEMC: Meets all applicablerequirements in EN61326-1Temperature Coefficients 0.1 x (specified accuracy)/ °C (<18 °C or >28 °C)HVAC Clamp Meter Specifications25Size (H X W X L) 9.1 x 3.8 x 1.7 inches (240 x 80 x 40 mm) Weight1.1 lb (310 g)Design Standards and Compliance IEC 61010, IEC 61010-2-032,CEAgency ApprovalsP ) ; sN10140Over-voltage Category600 V, CAT III per IEC 1010-1CAT III equipment is designed to protect against transients in equipment in fixed-equipment installations, such asdistribution panels, feeders and short branch circuits, and lighting systems in large buildings.Power Requirements Two AA Batteries , NEDA 15 A, IEC LR61.888.610.7664**************************Fluke -Direct .com902Users Manual26**************************1.888.610.7664。
钢材标准对照表
sigla UNI vecchia/nuova
25Cr.Mo4 34Ni.Cr.Mo16 34Ni.Cr.Mo16 bonificato 35Cr.Mo4 35Cr.Mo4 bonificato 35Cr.Mo4 ricotto 38Ni.Cr.Mo4 39Ni.Cr.Mo3 bonificato 39Ni.Cr.Mo3 bonif./bonder. 39Ni.Cr.Mo3-Pb 39Ni.Cr.Mo3-Pb bonif. 40Cr.Mo4 42Cr.Mo4 bonificato AISI-02 (90Mn.Cr.V8) Api-5L/5LX AVP (Fe37A-Pb) C5+1%Ni C10 C15 C20 C30 C35 C40 C40 bonificato C40 bonderizzato C40 cromato C43 C45 C45 cromato C50 C50 temprato C60 C67 C67 temprato C75 C75 classe B C75 temprato C85 C90 C90 classe C C100 C100 classe D DH (C100 classe D) Domex* E235 (Fe35-1/Fe360) E255 (Fe45-1) E295 (Fe490) E355 (Fe510) ETG-100* (44S.Mn28)
norma UNI vecchia
7845 7845 7845 7845-7874-8787 7845-7874-8787 7845-7874-8787 5332/64 6403-7845-7874-ecc… 6403-7845-7874-ecc... 7845 7845 7845-8787 7845-8787 2955/3 Ansi-B36.10 (Usa) 468 Astm-A217 (Usa) 7846-8550 7846-8550 7874 7845-7874 7845 7231-7845 7845-7847-7874-8787 7845-7847-7874-8787 7231-7845 7845-7847-7874-8787 7847 7231-7845 7845-8551-Iso683/1 7845-8551-Iso683/1 7845 7845 7845 7064 7064 8893 7064 3545/68 8893 8893 7064 7064 (C85) 3545/68 7064 3545/68 (Uni-En-10270-1 nuova) non esiste (Uni-En-10297-1 nuova) (Uni-En-10305-1 nuova) (Uni-En-10025 nuova) (Uni-En-10297-1 nuova) non esiste varie
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Product Name:
Pizotifen malate CAS No.:
######Cat. No.:
HY-B0115A
Product Data Sheet
MWt:
429.53Formula:
C23H27NO5S Purity :>98%
Solubility:
DMSO 21 mg/mL; Water <1 mg/mL
Mechanisms:Biological Activity:
Pizotifen malate is a highly selective 5-HT receptor blocking agent which is a benzocycloheptane
Pathways:GPCR/G protein; Target:5-HT Receptor
Pathways:Neuronal Signaling; Target:5-HT Receptor Pizotifen malate is a highly selective 5HT receptor blocking agent, which is a benzocycloheptane
based drug.
Target: 5-HT receptor Pizotifen, malate is a benzocycloheptane based drug used as a medicine, primarily as a
preventative to reduce the frequency of recurrent migraine headaches [1]. Pizotifen malate proved to be a highly selective 5-HT receptor blocking agent both in vivo (on saphenous veins) and in vitro (on
saphenous veins and basilar arteries) from dogs and humans [2].Three groups of pregnant mice were treated daily with oral doses (0.24, 0.6 and 1.2 mg/kg) of
pipethiadene and pizotifen malate from day 4 to day 16 of gestation. The following parameters were References:
[1]. Stark RJ, et al. Management of migraine in Australian general practice. Med J Aust. 2007 Aug
6;187(3):142-6.[2]. Müller-Schweinitzer E, Pizotifen, an antimigraine drug with venoconstrictor activity in vivo. J investigated: reproductive health of the dams, external, skeletal and visceral malformations of
fetuses and frequencies of micronuclei and chromosome aberrations in bone marrow cells of dams.Oral admi...
[],,g g y
Cardiovasc Pharmacol. 1986 Jul-Aug;8(4):805-10.[3]. Ujházy E, et al. Teratological and cytogenetical evaluation of two antihistamines (pipethiadene
and pizotifen maleate) in mice. Agents Actions. 1988 Apr;23(3-4):376-8.Caution: Not fully tested. For research purposes only
Medchemexpress LLC
18W i l k i n s o n W a y , P r i n c e t o n , N J 08540,U S A
E m a i l : i n f o @m e d c h e m e x p r e s s .c o m W e b : w w w .m e d c h e m e x p r e s s .c o m。