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纳米零价铁材料的表面修饰及其检测典型环境污染物

纳米零价铁材料的表面修饰及其检测典型环境污染物

Surface modification of nanoscale zerovalent iron particles and their applications in enrichment and determination of typicalenvironmental pollutantsABSTRACTSafarikova and Safarik first proposed magnetic solid phase extraction (MSPE) in 1999, and introduced it into environmental sample pre-treatment field. As a new mode of SPE. Magnetic solid phase extraction has merits such as simplicity, rapidness, high enrichment factor, short extraction time and low consumption of organic solvents. Nanoscale zero-valent iron (NZVI) has been widely used in environmental engineering field due to its high reactivity, large specific surface area, and abundant reactive sites, but there are very few reports on its applications in environmental analytical chemistry due to the fact that bare NZVI particles are easily oxided and agglomerated. Functional modification is a good way to enhance the various applicabilities for various pollutants and dispersities, and which make NZVI have great application prospect in the field of environmental analytical chemistry.In this paper, NZVI particles are modified with several functional materials. The new synthesized magnetic nanoparticles are investigated as the magnetic solid phase extraction adsorbents for preconcentration and determination of typical environmental pollutants prior to high performance liquid chromatography coupled with variable wavelength detection. The main contents are as follows:(1) Fe@MgAl-LDH magnetic nanoparticles were synthesized and characterized with TEM, XRD techniques, which was investigated to develop a novel method for proconcentration and determination of bisphenol A, 4-octylphenol and 4-nonylphenol from environmental water samples. Under the optimal conditions, linear ranges were obtained in the range of 0.5-200 μg·L-1, the detection limits were in the range of 0.24-0.34 μg·L-1, and the spiked recoveries were achieved in the range of 96.0-99.3%.(2) Fe@MOF-5 magnetic nanoparticles were prepared and characterized with TEM, EDS, XRD techniques. This new magnetic material was used as the adsorbent todevelop a new method for enrichmment and determination of carbazole, 9-methylcarbazole, dibenzothiophene, 4-methyldibenzothiophene and 4,6-dimethyldibenzothiophene from environmental water samples. Under the optimal conditions, there were good linearship between the concentration and the peak area in the range of 0.05-200 μg·L-1, the detection limits were in the range of 0.025-0.033 μg·L-1. Real water samples were used to validate the applicability of the proposed method, the spiked recoveries were in the range of 92.6-97.3%.(3) Fe@MIL-101(Cr) magnetic nanoparticles were prepared and characterized with TEM, EDS, XRD techniques, which was used to establish a magnetic solid phase method for proconcentration and determination of naphthalene, anthracene, fluoranthene and pyrene from environmental water samples. Under the optimal conditions, linear ranges were achieved in the range of 0.1-500 μg·L-1, the detection limits were in the range of 0.044-0.064 μg·L-1, and the spiked recoveries were in the range of 85.7-97.3%.(4) Fe@Ag@DMB magnetic nanoparticles were synthesized and characterized with TEM, EDS techniques. This new material was used to develop a novel method for simultaneous proconcentration and determination of Pb(II), Cd(II) and Hg(II) ions from environmental water samples. Under the optimal conditions, linear ranges were in the range of 0.05-200 μg·L-1, the detection limits were in the range of 0.011-0.031 μg·L-1, and the spiked recoveries were in the range of 97.5-103.2%.(5) Magnetic and thermos dual-responsive Fe@SiO2@p(NIPAM-co-DMMA) nanoparticles were prepared and characterized with TEM, XRD techniques, which was used as the adsorbent for developing a novel method for proconcentration and determination of four Sudan Red dyes from environmental water samples. Under the optimal conditions, linear ranges were obtained in the range of 0.05-500 μg·L-1, the detection limits were in the range of 0.005-0.013 μg·L-1, and the spiked recoveries were in the range of 97.4-102.6%.Keywords:nanoscale zero-valent iron core-shell materials;phenols;polycyclic aromatic hydrocarbons;heavy metal ions;Sudan Red dyes目录硕士学位论文独创性声明 (I)硕士学位论文版权使用授权书 (I)摘要 (II)ABSTRACT (IV)第1章绪论 (1)1.1 磁性固相萃取技术 (1)1.2 磁性纳米颗粒表面修饰及功能化 (2)1.2.1 碳材料 (2)1.2.2 聚合物 (3)1.2.3 金属有机骨架材料 (4)1.2.4 氧化物 (5)1.3 磁性材料在环境污染物检测中的应用 (6)1.3.1 多环芳烃 (6)1.3.2 重金属离子 (7)1.3.3 染料 (8)1.3.4 农药 (8)1.3.3 酚类化合物 (9)1.4 论文构思及研究内容 (10)1.4.1 研究内容 (10)1.4.2 技术路线 (10)1.4.3 研究总工作量 (11)第2章磁性固相萃取-高效液相色谱法测定环境水样中的双酚A、4-辛基酚和4-壬基酚 (12)2.1 实验部分 (12)2.1.1 试剂和仪器 (12)2.1.2 Fe@MgAl-LDH的制备 (13)2.1.3 磁性固相萃取(MSPE)过程 (13)2.1.4 高效液相色谱仪(HPLC)分析 (14)2.2 结果与讨论 (14)2.2.1 材料表征 (14)2.2.2 实验条件的优化 (15)2.2.3 材料可重复利用性研究 (22)2.2.4 方法评价和实际水样分析 (23)2.3 小结 (25)第3章金属有机骨架材料MOF-5修饰纳米零价铁磁性固相萃取富集检测痕量含氮及含硫多环芳烃 (26)3.1 实验部分 (26)3.1.1 试剂和仪器 (26)3.1.2 Fe@MOF-5的制备 (27)3.1.3 磁性固相萃取(MSPE)过程 (28)3.1.4 高效液相色谱仪(HPLC)分析 (29)3.2 结果与讨论 (29)3.2.1 材料表征 (29)3.2.2 实验条件的优化 (31)3.2.3 材料可重复利用性研究 (37)3.2.4 方法评价和实际水样分析 (37)3.3 小结 (40)第4章金属有机骨架材料修饰纳米零价铁磁性固相萃取富集检测环境水样中痕量多环芳烃 (41)4.1 实验部分 (41)4.1.1 试剂和仪器 (41)4.1.2 Fe@MIL-101(Cr)的制备 (42)4.1.3 磁性固相萃取(MSPE)过程 (43)4.1.4 高效液相色谱仪(HPLC)分析 (43)4.2 结果与讨论 (43)4.2.1 材料表征 (43)4.2.2 实验条件的优化 (45)4.2.3 材料可重复利用性研究 (52)4.2.4 方法评价和实际水样分析 (53)4.3 小结 (55)第5章Fe@Ag@DMB磁性固相萃取高效液相色谱联用同时检测水中Pb(II)、Cd(II)和Hg(II) (56)5.1 实验部分 (56)5.1.1 试剂和仪器 (56)5.1.2 Fe@Ag@DMB的制备 (57)5.1.3 磁性固相萃取(MSPE)过程 (57)5.1.4 高效液相色谱仪(HPLC)分析 (58)5.2 结果与讨论 (58)5.2.1 材料表征 (58)5.2.2 实验条件的优化 (59)5.2.3 材料可重复利用性研究 (64)5.2.4 方法评价和实际水样分析 (64)5.3 小结 (66)第6章Fe@SiO2@p(NIPAM-co-DMMA) 磁性温度双重响应材料富集检测环境水样中的苏丹红染料 (67)6.1 实验部分 (67)6.1.1 试剂和仪器 (67)6.1.2 Fe@SiO2@p(NIPAM-co-DMMA)的制备 (68)6.1.3 磁性固相萃取(MSPE)过程 (68)6.1.4 高效液相色谱仪(HPLC)分析 (69)6.2 结果与讨论 (69)6.2.1 材料表征 (69)6.2.2 实验条件的优化 (71)6.2.3 材料可重复利用性研究 (78)6.2.4 方法评价和实际水样分析 (78)6.3 小结 (80)第7章结论 (81)参考文献 (83)致谢 (95)攻读硕士期间发表论文情况 (96)第1章绪论目前环境污染是全世界共同关注的问题。

高密度脂蛋白胆固醇测定试剂盒(直接法-过氧化氢酶清除法)产品技术要求meigaoyi

高密度脂蛋白胆固醇测定试剂盒(直接法-过氧化氢酶清除法)产品技术要求meigaoyi

高密度脂蛋白胆固醇测定试剂盒(直接法-过氧化氢酶清除法) 适用范围:用于体外定量检测人血清中高密度脂蛋白胆固醇浓度。

1.1包装规格a) 试剂1:2×60ml,试剂2:2×20ml;b) 试剂1:4×60ml,试剂2:4×20ml;c) 试剂1:3×60ml,试剂2:3×20ml;d) 试剂1:2×45ml,试剂2:2×15ml;e)试剂1:1×45ml,试剂2:1×15ml;f)试剂1:2×16.8ml,试剂2:2×5.6ml;g)试剂1:2×300ml,试剂2:2×100ml。

1.2主要组成成分试剂1主要组成成分试剂2主要组成成分2.1 外观和性状2.1.1 试剂盒各组分应齐全、完整、液体无渗漏;外包装完好、无破损,标签完好、字迹清晰。

2.1.2 试剂1应为无色或淡黄色透明溶液;试剂2应为无色或淡黄色透明溶液。

2.2 净含量应不低于试剂瓶标示装量。

2.3 试剂空白吸光度测定试剂空白吸光度,应<0.05。

2.4 分析灵敏度测试1.0mmol/L样本时,吸光度变化(△A)应大于0.04。

2.5 准确度用参考物质(GBW09178-GBW09180)对试剂(盒)进行测试,测定值与靶值相对偏差不超过±10%。

2.6 精密度2.6.1重复性在重复性条件下,测试浓度正常值和高值的样本,各重复测定不少于10次,变异系数(CV)应不超过4%。

2.6.2批间差抽取3个不同批号试剂,测试同一浓度的样品进行重复检测,每个批号试剂检测3次,批间相对极差应不大于10%。

2.7 线性试剂盒线性在(0.05,3.90)mmol/L范围内:2.7.1线性回归的相关系数应不低于0.995;2.7.2 (0.05,1.00]mmol/L区间内,线性偏差不超过±0.1mmol/L2.7.3(1.00,3.90)mmol/L区间内,线性偏差不超过±10%。

GLP-1诱导表达的原理

GLP-1诱导表达的原理

GLP-1诱导表达的原理三磷酸鸟苷环化水解酶(GTPCH)Ⅰ是四氢生物蝶呤(BH4)合成的关键限速酶,BH4是内皮一氧化氮合酶(eNOS)的辅助因子。

糖尿病等病理条件下,BH4水平下降促使eNOS脱偶联,导致内皮功能异常。

胰高糖素样肽(GLP-1)可增强内皮eNOS的磷酸化、增加NO、发挥内皮保护作用。

该课题组前期研究显示,同型半胱氨酸可抑制内皮BH4水平、诱导eNOS脱偶联,损害患者冠脉内皮功能。

预实验显示,GLP-1受体激动剂可促进内皮细胞eNOS表达、抑制氧化应激、增加NO。

该研究分别在内皮细胞及apoE敲除小鼠整体水平,探讨GLP-1受体激动剂能否通过上调血管内皮GTPCH1水平、增加BH4、抑制氧化应激、促使eNOS复偶联等机制,发挥血管内皮保护作用,并在临床水平进一步验证。

实验结果显示GLP-1受体激动剂艾塞那肽可以改善内皮细胞氧化损伤,可能是通过激活AMPK途径增加GTPCH1表达,从而上调BH4水平,进而促进eNOS复偶联,NO产生增加,最终达到改善内皮功能的作用。

在ApoE小鼠中持续应用艾塞那肽(8周)能够显著提高主动脉中GTPCH-1的表达水平和BH4的含量,并且使得主动脉eNOS的磷酸化水平相应增高,提高eNOS的活性,使之能够保持并较好地发挥催化生成血管内皮舒张因子NO的功能,最终增加血管内NO的生成同时减少NO的损失,改善血管内皮依赖性舒张功能,从而达到保护内皮功能的作用。

临床研究显示艾塞那肽不仅能降低2型糖尿病患者的血糖,改善胰岛细胞功能,降低患者的体重、体重指数、腹围,调节血脂代谢,还可具有改善血管内皮功能,提高冠脉内血流发挥血管内皮保护作用,减少糖尿病患者心血管不良事件的发生。

GLP-1受体激动剂,通过上调内皮细胞GTPCH1表达、增加BH4水平、促使eNOS复偶联、增加NO,进而改善血管内皮功能;并可通过激活AMPK,反转被抑制的内皮细胞GTPCH1表达,增加BH4水平,促使eNOS复偶联,改善内皮依赖的血管功能,发挥血管保护作用。

Solarbio BH3 结构域凋亡诱导蛋白 ELISA 试剂盒说明书

Solarbio BH3 结构域凋亡诱导蛋白 ELISA 试剂盒说明书

人BH3结构域凋亡诱导蛋白ELISA试剂盒产品编号:SEKH-0104该试剂盒用于定量检测血清、血浆或细胞上清等样本中人BID的浓度仅供研究,不用于临床诊断订购热线:400-968-6088﹡技术支持邮箱:公司官网:目录背景介绍 (1)检测原理 (1)检测原理图 (2)注意事项 (2)技术要点 (3)试剂盒组成及储存 (4)自备实验器材(不提供,可代购) (4)样本收集及储存 (5)试剂准备 (6)检测步骤 (8)操作流程图 (10)结果计算 (11)典型数据 (11)常见问题及解决方法 (14)相关产品 (15)发表优秀文献 (15)BH3相互作用结构域死亡激动剂(BID)是Bcl-2蛋白家族的促凋亡成员,该家族仅包含BH3结构域,它与Bcl-2家族蛋白相互作用并具有促死亡活性是必需的。

BID对这些蛋白酶介导的细胞死亡很重要,因此是蛋白酶介导的死亡信号的前哨。

最近的研究进一步表明,Bid可能不仅仅是一个杀伤分子,它还可能通过参与有丝分裂检查点来维持基因组的稳定性。

BID是内在死亡通路的整合关键调控因子,可放大caspase 依赖和caspase不依赖神经元凋亡的执行。

因此,药理学抑制BID为程序性细胞死亡突出的神经系统疾病提供了一种有前途的治疗策略。

Fas/TNF-R1死亡受体激活后,BID被Caspase8激活。

被激活的BID被转移到线粒体并诱导细胞色素c释放,从而激活下游的半胱天冬酶。

BID 的作用已被提出涉及其截断形式tBid的线粒体重新定位,以促进细胞色素c等凋亡蛋白的释放。

检测原理Solarbio(Solarbio®)ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。

将抗人BID单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的人BID会与酶标板上的包被抗体充分结合;洗板后加入HRP酶标记的抗人BID抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的人BID发生特异性结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的人BID,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450nm(OD=450nm)处测定反应孔样品吸光度(OD),样本中的人BID浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中人BID的浓度。

SDSE所有详细试剂配方

SDSE所有详细试剂配方

SDSE所有详细试剂配方SDSE(Sodium dodecyl sulfate-electrolyte system)是一种常用的聚丙烯酰胺凝胶电泳缓冲液,主要用于蛋白质电泳分离。

SDSE的制备是根据一定的配方进行的,下面是SDSE的详细试剂配方:1.氯化钠(NaCl):18.3g溶解在1L去离子水中2.磷酸二氢钠(NaH2PO4):3.5g溶解在1L去离子水中3. 溴酚蓝(Bromophenol Blue):0.25g溶解在10mL水中4. SDS(Sodium Dodecyl Sulfate):10g溶解在1L去离子水中5. 甘油(Glycerol):10mL溶解在100mL去离子水中以上是SDSE的基础配方,下面是其制备步骤:1.将NaCl和NaH2PO4溶解在分别容量为1L的去离子水中,用磁力搅拌器搅拌均匀。

2.分别将溶解好的NaCl和NaH2PO4溶液倒入大容量烧杯中,混合均匀。

3.在pH计的帮助下调节缓冲液的pH值,使其在6.8-7.0之间。

4.加入溴酚蓝溶液,此时溴酚蓝的浓度为0.0025%,用磁力搅拌器搅拌均匀。

5.加入SDS溶液,用磁力搅拌器搅拌均匀。

注意,加入SDS时要小心,避免溅出。

6.最后加入甘油溶液,搅拌均匀。

甘油的添加主要是为了增加样品的密度,使其在凝胶中下沉更快。

SDSE的配方比较简单,主要成分包括NaCl、NaH2PO4、溴酚蓝、SDS和甘油。

其中,NaCl和NaH2PO4是缓冲液的主要组成部分,用于维持适当的离子强度和pH值;溴酚蓝用于着色样品,方便观察电泳结果;SDS主要起到断开蛋白质的二级结构和线性化作用;甘油则用于增加样品密度,使其在凝胶中下沉更快。

通过恰当的配方和制备步骤,可以获得高质量的SDSE缓冲液,用于蛋白质电泳分离和分析。

但需要注意的是,不同实验目的和要求可能需要微调配方中一些成分的浓度,因此在具体实验过程中,可以根据需要进行相应的调整。

核酸提取裂解液的成分 -回复

核酸提取裂解液的成分 -回复

核酸提取裂解液的成分-回复核酸提取裂解液是一种常用于生物学实验中的溶液,用于裂解细胞并释放出细胞内的核酸。

它的成分通常由不同的试剂组成,每种试剂都有其特定的作用和功能。

在核酸提取裂解液中,最常见的成分是细胞裂解剂。

这些裂解剂有助于破坏细胞膜和核膜,使细胞内的核酸暴露在溶液中。

常见的细胞裂解剂包括胰酶、蛋白酶K和SDS(十二烷基硫酸钠)。

胰酶和蛋白酶K可以降解蛋白质,从而破坏细胞膜和核膜。

SDS可以破坏细胞膜,并使细胞内的蛋白质变为可溶性。

另一个常见的成分是蛋白酶抑制剂。

蛋白酶是一种能够降解蛋白质的酶类,而核酸提取实验中需要保护细胞内的蛋白质,避免其被蛋白酶降解。

因此,蛋白酶抑制剂可以抑制蛋白酶的活性,保护细胞内的蛋白质。

常见的蛋白酶抑制剂包括苯甲烷磺酰氟化合物(PMSF)、乙锡硫酰胺(DTT)和EDTA (乙二胺四乙酸)。

PMSF可以抑制广谱的蛋白酶,DTT可以还原氧化的蛋白质,而EDTA则可以结合金属离子,抑制某些金属离子依赖的蛋白酶。

此外,核酸提取裂解液中还常常添加缓冲液。

缓冲液可以调整溶液的酸碱度,使其维持在适宜的范围内。

常见的缓冲液有Tris-HCl缓冲液、PBS(磷酸盐缓冲液)和TE缓冲液(Tris-EDTA缓冲液)。

这些缓冲液可以维持细胞裂解液的pH值稳定,确保核酸提取的成功。

另外,核酸提取裂解液中还可以添加辅助剂,以增加核酸的提取效率。

辅助剂可以包括盐、酒精和有机溶剂等。

盐可以调节离子强度,有助于核酸的溶解和沉淀。

酒精和有机溶剂可以用于沉淀核酸,使其从溶液中析出。

最后,核酸提取裂解液中可能还会添加其他的试剂,例如胆固醇、RNA酶抑制剂和DNA稳定剂等。

这些试剂可以根据实验需要进行调整,以增加裂解效果或保护核酸的稳定性。

总之,核酸提取裂解液的成分多种多样,不同的试剂组合可以根据实验的需要进行调整。

这些试剂共同作用,能够有效地裂解细胞并释放出细胞内的核酸,为后续的核酸提取和分析提供可靠的基础。

BH3类似物促细胞凋亡机制及临床研究进展

BH3类似物促细胞凋亡机制及临床研究进展

BH3类似物促细胞凋亡机制及临床研究进展张岳;张伊佳;李学军【期刊名称】《中国药理学与毒理学杂志》【年(卷),期】2017(031)001【摘要】Targeting cell apoptosis is currently the most promising therapy for cancer treatment. The BH3-only protein, which is a member of Bcl-2 family, can bind to the pro-survival members of the family and neutralize their functional activities to induce apoptosis (ie, to isolate pro-apoptotic members of the Bcl-2 family). BH3 mimetics, a kind of small molecule compounds, has the ability to mimic the BH3-only protein to induce apoptosis. The prototype of BH3 mimetics is ABT-737, who can selectively targets on BCL-XL, BCL-2 and BCL-W (but not MCL-1 and A1). ABT-263, a derivative of ABT-737, has a better performance of inducing apoptosis and inhibiting the growth of tumor in clinical trials. At this stage, some presumably BH3 mimetics has entered the clinical stage, while a large part of them is still being characterized and tested. Basing on the mechanism of BH3-only protein, this review summarize a variety of BH3 mimetics which have been widely recognized, and show the latest developments of newly diagnosed BH3 mimetics in the field.%靶向细胞凋亡是目前癌症治疗中最具发展前景的治疗方法之一。

碧云天生物技术碘化丙啶产品说明书

碧云天生物技术碘化丙啶产品说明书

碧云天生物技术/Beyotime Biotechnology订货热线:400-168-3301或800-8283301订货e-mail:******************技术咨询:*****************网址:碧云天网站微信公众号Propidium Iodide/碘化丙啶产品编号产品名称包装ST512 Propidium Iodide/碘化丙啶20mg产品简介:Propidium Iodide简称PI,中文名为碘化丙啶。

分子式为C27H34I2N4,分子量为668.40,纯度>95%。

进口分装,常用于细胞凋亡(apoptosis)或细胞坏死(necrosis)的检测,常用于流式细胞仪分析。

包装清单:产品编号产品名称包装ST512 Propidium Iodide/碘化丙啶20mg—说明书1份保存条件:4ºC避光保存。

注意事项:本产品对人体有刺激性,操作时请小心,并注意适当防护以避免直接接触人体或吸入体内。

本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。

为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用本产品的文献:1.Wu G, Liu ZS, Qian Q, Jiang CQ. Effects of Berberine on the Growth ofHepatocellular Carcinoma Cell lines. Medical Journal of Wuhan University. 2008 Jan;29(1):102-105.2.Liu Y, Sheng Z, Liu H, Wen D, He Q, Wang S, Shao W, Jiang RJ, An S,Sun Y, Bendena WG, Wang J, Gilbert LI, Wilson TG, Song Q, Li S.Juvenile hormone counteracts the bHLH-PAS transcription factors MET and GCE to prevent caspase-dependent programmed cell death in Drosophila. Development. 2009 Jun;136(12):2015-25.3.Li DL, Liu JJ, Liu BH, Hu H, Sun L, Miao Y, Xu HF, Yu XJ, Ma X, RenJ, Zang WJ. Acetylcholine inhibits hypoxia-induced tumor necrosis factor-α production via regulation of MAPKsphosphorylation in cardiomyocytes. J Cell Physiol. 2011 Apr;226(4):1052-9.4.Cao X, Deng W, Wei Y, Su W, Yang Y, Wei Y, Yu J, Xu X. Encapsulationof plasmid DNA in calcium phosphate nanoparticles: stem cell uptake and genetransfer efficiency. Int J Nanomedicine. 2011;6:3335-49.5.Meng LY, Liu HR, Shen Y, Yu YQ, Tao X. Cochinchina momordica seedextract induces G2/M arrest and apoptosis in human breast cancerMDA-MB-231 cells by modulating the PI3K/Akt pathway. Asian Pac J Cancer Prev. 2011;12(12):3483-8.6.Zhao Q, Xue Y, Wang JF, Li H, Long TT, Li Z, Wang YM, Dong P, XueCH. In vitro and in vivo anti-tumour activities of echinoside A and ds-echinoside A from Pearsonothuriagraeffei. J Sci Food Agric. 2012 Mar 15;92(4):965-74.7.Tu Z, Ma Y, Tian J, Li H, Akers W, Achilefu S, Gu Y. Estrogen receptor βpotentiates the antiproliferative effect of raloxifene and affects the cellmigration and invasion in HCT-116 colon cancer cells. J Cancer Res Clin Oncol. 2012 Jul;138(7):1091-103.8.Zhou Z, Wan Y, Zhang Y, Wang Z, Jia R, Fan Y, Nie H, Ying S, Huang P,Wang F. Follicular development and expression of nuclear respiratory factor-1 and peroxisome proliferator-activated receptor γ coactivator-1 alpha in ovaries of fetal and neonatal doelings. J Anim Sci. 2012 Nov;90(11):3752-61.9.Jun Fang, Meihu Ma, Yongguo Jin, Ning Qiu, Chan Wang, Guodong Renand Xin Huang. Assessment of Salmonella enteritidis Viability in Egg White during Early Incubation Stages by Fluorescent Staining Method.Asian Journal of Animal and Veterinary Advances. 7: 556-67.10.Zhen-Jun S, Yuan-Yuan Z, Ying-Ying F, Shao-Ju J, Jiao Y, Xiao-Wei Z,Jian C, Yao X, Li-Ming Z.β,β-Dimethylacrylshikonin exerts antitumoractivity via Notch-1 signaling pathway in vitro and invivo. Biochem Pharmacol. 2012 Aug 15;84(4):507-12.11.Tu Z, Li H, Ma Y, Tang B, Tian J, Akers W, Achilefu S, Gu Y. Theenhanced ant iproliferative response to combined treatment of trichostatin A with raloxifene in MCF-7 breast cancer cells and its relevance to estro gen receptor β expression. Mol Cell Biochem. 2012 Jul;366(1-2):111-22.12.Feng C, Xu Z, Li Z, Zhang D, Liu Q, Lu L. Down-regulation of Wnt10aby RNA interference inhibits proliferation and promotes apoptosis in mouse embryonic palatal mesenchymal cells throu gh Wnt/β-catenin signaling pathway. J Physiol Biochem. 2013 Dec;69(4):855-63.13.Hou Y, Chu M, Du FF, Lei JY, Chen Y, Zhu RY, Gong XH, Ma X, Jin J.Recombinant disintegrin domain of ADAM15 inhibits the proliferation and migration of Bel-7402 cells. Biochem Biophys Res Commun. 2013 Jun 14;435(4):640-5.14.Li Q, Zhou X, Shi Y, Li J, Zheng L, Cui L, Zhang J, Wang L, Han Z, HanY, Fan D. In vivo tracking and comparison of the therapeutic effects of MSCs and HSCs for liver injury. PLoS One. 2013 Apr 30;8(4):e62363. 15.Zhou R, Huang W, Yao Y, Wang Y, Li Z, Shao B, Zhong J, Tang M,Liang S, Zhao X, Tong A, Yang J.CA II, a potential biomarker by proteomic analysis, exerts significant inhibitory effect on the growth of colorectal cancer cells. Int J Oncol. 2013 Aug;43(2):611-21.16.Wang Y, Jiang XL, Peng SW, Guo XY, Shang GG, Chen JC, Wu Q, ChenGQ. Induced apoptosis of osteoblasts proliferating on polyhydroxyal kanoates. Biomaterials. 2013 May;34(15):3737-46.17.Yang F, Huang W, Li Y, Liu S, Jin M, Wang Y, Jia L, Gao Z. Anti-tumoreffects in mice induced by survivin-targeted siRNA delivered through polysaccharide nanoparticles. Biomaterials. 2013 Jul;34(22):5689-99.18.Zhou S, Wu H, Zeng C, Xiong X, Tang S, Tang Z, Sun X. ApolipoproteinE protects astrocytes from hypoxia and glutamate-induced apoptosis.FEBS Lett. 2013 Jan 16;587(2):254-8.19.Li R, Luo X, Li L, Peng Q, Yang Y, Zhao L, Ma M, Hou Z. TheProtective Effects of Melatonin Against Oxidative Stress and Inflammation Induced by AcuteCadmium Exposure in Mice Testis. Biol Trace Elem Res. 2016 Mar;170(1):152-64.Version 2016.12.08。

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Inhibitors, Agonists, Screening LibrariesSafety Data Sheet Revision Date:Oct.-01-2018Print Date:Oct.-01-20181. PRODUCT AND COMPANY IDENTIFICATION1.1 Product identifierProduct name :BH3I-1Catalog No. :HY-100383CAS No. :300817-68-91.2 Relevant identified uses of the substance or mixture and uses advised againstIdentified uses :Laboratory chemicals, manufacture of substances.1.3 Details of the supplier of the safety data sheetCompany:MedChemExpress USATel:609-228-6898Fax:609-228-5909E-mail:sales@1.4 Emergency telephone numberEmergency Phone #:609-228-68982. HAZARDS IDENTIFICATION2.1 Classification of the substance or mixtureGHS Classification in accordance with 29 CFR 1910 (OSHA HCS)Acute toxicity, Oral (Category 4), H302Acute toxicity, Inhalation (Category 4), H332Acute toxicity, Dermal (Category 4), H3122.2 GHS Label elements, including precautionary statementsPictogramSignal word WarningHazard statement(s)H302 + H312 + H332 Harmful if swallowed, in contact with skin or if inhaledPrecautionary statement(s)P261 Avoid breathing dust ⁄ fume ⁄ gas ⁄ mist ⁄ vapours ⁄ spray.P264 Wash skin thoroughly after handling.P270 Do not eat, drink or smoke when using this product.P271 Use only outdoors or in a well-ventilated area.P280 Wear protective gloves ⁄ protective clothing.P301 + P312 IF SWALLOWED: Call a POISON CENTER ⁄doctor if you feel unwell.P302 + P352 IF ON SKIN: Wash with plenty of soap and water.P304 + P340 IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.P312 Call a POISON CENTER ⁄doctor if you feel unwell.P322 Specific measures (see supplemental first aid instructions on this label).P330 Rinse mouth.P363 Wash contaminated clothing before reuse.P501 Dispose of contents ⁄ container to an approved waste disposal plant2.3 Other hazardsNone.3. COMPOSITION/INFORMATION ON INGREDIENTS3.1 SubstancesSynonyms:BHI1;BHI-1;BHI 1;BH 3I1Formula:C15H14BrNO3S2Molecular Weight:400.31CAS No. :300817-68-94. FIRST AID MEASURES4.1 Description of first aid measuresEye contactRemove any contact lenses, locate eye-wash station, and flush eyes immediately with large amounts of water. Separate eyelids with fingers to ensure adequate flushing. Promptly call a physician.Skin contactRinse skin thoroughly with large amounts of water. Remove contaminated clothing and shoes and call a physician.InhalationImmediately relocate self or casualty to fresh air. If breathing is difficult, give cardiopulmonary resuscitation (CPR). Avoid mouth-to-mouth resuscitation.IngestionWash out mouth with water; Do NOT induce vomiting; call a physician.4.2 Most important symptoms and effects, both acute and delayedThe most important known symptoms and effects are described in the labelling (see section 2.2).4.3 Indication of any immediate medical attention and special treatment neededTreat symptomatically.5. FIRE FIGHTING MEASURES5.1 Extinguishing mediaSuitable extinguishing mediaUse water spray, dry chemical, foam, and carbon dioxide fire extinguisher.5.2 Special hazards arising from the substance or mixtureDuring combustion, may emit irritant fumes.5.3 Advice for firefightersWear self-contained breathing apparatus and protective clothing.6. ACCIDENTAL RELEASE MEASURES6.1 Personal precautions, protective equipment and emergency proceduresUse full personal protective equipment. Avoid breathing vapors, mist, dust or gas. Ensure adequate ventilation. Evacuate personnel to safe areas.Refer to protective measures listed in sections 8.6.2 Environmental precautionsTry to prevent further leakage or spillage. Keep the product away from drains or water courses.6.3 Methods and materials for containment and cleaning upAbsorb solutions with finely-powdered liquid-binding material (diatomite, universal binders); Decontaminate surfaces and equipment by scrubbing with alcohol; Dispose of contaminated material according to Section 13.7. HANDLING AND STORAGE7.1 Precautions for safe handlingAvoid inhalation, contact with eyes and skin. Avoid dust and aerosol formation. Use only in areas with appropriate exhaust ventilation.7.2 Conditions for safe storage, including any incompatibilitiesKeep container tightly sealed in cool, well-ventilated area. Keep away from direct sunlight and sources of ignition.Recommended storage temperature:Powder-20°C 3 years4°C 2 yearsIn solvent-80°C 6 months-20°C 1 monthShipping at room temperature if less than 2 weeks.7.3 Specific end use(s)No data available.8. EXPOSURE CONTROLS/PERSONAL PROTECTION8.1 Control parametersComponents with workplace control parametersThis product contains no substances with occupational exposure limit values.8.2 Exposure controlsEngineering controlsEnsure adequate ventilation. Provide accessible safety shower and eye wash station.Personal protective equipmentEye protection Safety goggles with side-shields.Hand protection Protective gloves.Skin and body protection Impervious clothing.Respiratory protection Suitable respirator.Environmental exposure controls Keep the product away from drains, water courses or the soil. Cleanspillages in a safe way as soon as possible.9. PHYSICAL AND CHEMICAL PROPERTIES9.1 Information on basic physical and chemical propertiesAppearance Light yellow to yellow (Solid)Odor No data availableOdor threshold No data availablepH No data availableMelting/freezing point No data availableBoiling point/range No data availableFlash point No data availableEvaporation rate No data availableFlammability (solid, gas)No data availableUpper/lower flammability or explosive limits No data availableVapor pressure No data availableVapor density No data availableRelative density No data availableWater Solubility No data availablePartition coefficient No data availableAuto-ignition temperature No data availableDecomposition temperature No data availableViscosity No data availableExplosive properties No data availableOxidizing properties No data available9.2 Other safety informationNo data available.10. STABILITY AND REACTIVITY10.1 ReactivityNo data available.10.2 Chemical stabilityStable under recommended storage conditions.10.3 Possibility of hazardous reactionsNo data available.10.4 Conditions to avoidNo data available.10.5 Incompatible materialsStrong acids/alkalis, strong oxidising/reducing agents.10.6 Hazardous decomposition productsUnder fire conditions, may decompose and emit toxic fumes.Other decomposition products - no data available.11.TOXICOLOGICAL INFORMATION11.1 Information on toxicological effectsAcute toxicityClassified based on available data. For more details, see section 2Skin corrosion/irritationClassified based on available data. For more details, see section 2Serious eye damage/irritationClassified based on available data. For more details, see section 2Respiratory or skin sensitizationClassified based on available data. For more details, see section 2Germ cell mutagenicityClassified based on available data. For more details, see section 2CarcinogenicityIARC: No component of this product present at a level equal to or greater than 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.ACGIH: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by ACGIH.NTP: No component of this product present at a level equal to or greater than 0.1% is identified as a anticipated or confirmed carcinogen by NTP.OSHA: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by OSHA.Reproductive toxicityClassified based on available data. For more details, see section 2Specific target organ toxicity - single exposureClassified based on available data. For more details, see section 2Specific target organ toxicity - repeated exposureClassified based on available data. For more details, see section 2Aspiration hazardClassified based on available data. For more details, see section 212. ECOLOGICAL INFORMATION12.1 ToxicityNo data available.12.2 Persistence and degradabilityNo data available.12.3 Bioaccumlative potentialNo data available.12.4 Mobility in soilNo data available.12.5 Results of PBT and vPvB assessmentPBT/vPvB assessment unavailable as chemical safety assessment not required or not conducted.12.6 Other adverse effectsNo data available.13. DISPOSAL CONSIDERATIONS13.1 Waste treatment methodsProductDispose substance in accordance with prevailing country, federal, state and local regulations.Contaminated packagingConduct recycling or disposal in accordance with prevailing country, federal, state and local regulations.14. TRANSPORT INFORMATIONDOT (US)This substance is considered to be non-hazardous for transport.IMDGThis substance is considered to be non-hazardous for transport.IATAThis substance is considered to be non-hazardous for transport.15. REGULATORY INFORMATIONSARA 302 ComponentsNo chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.SARA 313 ComponentsThis material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis)reporting levels established by SARA Title III, Section 313.SARA 311/312 HazardsAcute Health Hazard16. OTHER INFORMATIONCopyright 2018 MedChemExpress. The above information is correct to the best of our present knowledge but does not purport to be all inclusive and should be used only as a guide. The product is for research use only and for experienced personnel. It must only be handled by suitably qualified experienced scientists in appropriately equipped and authorized facilities. The burden of safe use of this material rests entirely with the user. MedChemExpress disclaims all liability for any damage resulting from handling or from contact with this product.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。

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