Tofacitinib_citrate_NP-HPLC_10581_MedChemExpress

Crizotinib_hydrochloride_RP-HPLC_10558_MedChemExpress

VWD1 A, Wavelength=220 nm (D:\CHEM32\DATA\20151013\20151013 2015-10-13 08-25-43\030-1501.D) mAU 600 500 400 300 200 100 0 0 2 4 6 8 10 12 14 min 6.513 6.789 5.756
Data File D:\CHEM32\DATA\20151013\20151013 2015-10-13 08-25-43\001-1401.D Sample Name: CPK2015-O12-10558_0 ===================================================================== Acq. Operator : CYL(HPLC-09) Seq. Line : 14 Acq. Instrument : Instrument 1 Location : Vial 1 Injection Date : 10/13/2015 12:10:54 PM Inj : 1 Inj Volume : 5.000 µl Acq. Method : D:\CHEM32\DATA\20151013\20151013 2015-10-13 08-25-43\10-80A,220NM.M Last changed : 10/13/2015 12:09:32 PM by CYL(HPLC-09) (modified after loading) Analysis Method : D:\CHEM32\METHODS\RP-HPLC\10-80A(210NM).M Last changed : 10/13/2015 12:52:26 PM by CWQ(HPLC-09) (modified after loading) Catalog No : HY-50878A Batch#10558 A-RP-93 Additional Info : Peak(s) manually integrated

Tofacitinib_CP-690550_Tasocitinib_JAK STAT_JAK_CAS号477600-75-2说明书_AbMole中国

分子量312.37溶解性（25°C）DMSO 62 mg/mL分子式C H N O Water <1 mg/mLCAS号477600-75-2Ethanol <1 mg/mL储存条件3年 -20°C 粉末状生物活性Tofacitinib (CP-690550,Tasocitinib)是一种新型JAK3抑制剂，IC50为1 nM，作用于JAK2和JAK1选择性低20到100倍。

CP-690550 是特定的口服JAK3抑制剂, 作用于JAK2 和 JAK1,效果分别低20和100倍。

CP-690550 对30种其他激酶没有作用效果，平均IC50 > 3000 nM。

CP-690,550抑制IL-2诱导的增殖，比作用于GM-CSF诱导的增殖效果强30倍。

实验操作来自于公开的文献，仅供参考细胞实验细胞系cytokine-dependent NK92 cell line方法(A) After cytokine starvation for 24 hours, NK92 cells were stimulated by the addition of human IL-2 for 48 hours with and without the addition of serially increasing concentrations of tofacitinib. 3H-thymidine was added during the last 6 hours of the cultures. Cells werethen harvested and analyzed for 3H-thymidine incorporation. (B) Cytokine-starved NK92 cells were stimulated with human IL-2,combined IL-6/IL- 6R, or IL-12 for 48 hours with and without serially increasing concentrations of tofacitinib. (C) The NK92 cells weretreated as those in panel B with and without a single 50nM dose of tofacitinib. Data are presented as means ±SD (A-C) and arerepresentative of 3 independent experiments. (D) After cytokine starvation for 24 hours, NK92 cells were stimulated with 30 ng/mL ofIL-2, 100 ng/mL of combined IL-6/IL-6R, or 100 ng/mL of IL-12 for 1 hour with and without the addition of tofacitinib. The cell lysateswere immunoblotted with an anti–phospho-STAT5 monoclonal antibody and an anti-STAT5 antibody. ß-Actin was used as an inputcontrol. Data are representative of 3 independent experiments.浓度0~400 nM处理时间48 h动物实验动物模型IL-15–transgenic CD8 T-cell leukemia–bearing mice配制dissolved in polyethylene glycol 300 (PEG300; VWR Scientiﬁc Products)剂量50 mg/mL给药处理continuously administered via a subcutaneous mini-osmotic pump (ALZET)不同实验动物依据体表面积的等效剂量转换表（数据来源于FDA指南）小鼠大鼠兔豚鼠仓鼠狗重量 (kg)0.020.15 1.80.40.0810体表面积 (m)0.0070.0250.150.050.020.5K系数36128520动物 A (mg/kg) = 动物 B (mg/kg) × 动物 B的K系数动物 A的K系数Tofacitinib 目录号M1820化学数据162062mmm例如，依据体表面积折算法，将白藜芦醇用于小鼠的剂量22.4 mg/kg 换算成大鼠的剂量，需要将22.4 mg/kg 乘以小鼠的K系数（3），再除以大鼠的K系数m m （6），得到白藜芦醇用于大鼠的等效剂量为11.2 mg/kg。

Ilginatinib-NS-018-DataSheet-MedChemExpress

Ilginatinib (NS-018) (12.5, 25, 50, 100 mg/kg, p.o.) potently prolongs the survival of mice and reduces splenomegaly in a mouse Ba/F3-JAK2V617F disease model. Ilginatinib (NS-018) (25, 50 mg/kg, p.o.) significantly reduces leukocytosis, hepatosplenomegaly and extramedullary hematopoiesis, improves nutritional status, and prolongs survival in JAK2V617F transgenic mice [1].
REFERENCES
[1]. Nakaya Y, et al. Efficacy of NS-018, a potent and selective JAK2/Src inhibitor, in primary cells and mouse models of myeloproliferative neoplasms. Blood Cancer J. 2011 Jul;1(7):e29. [2]. Kuroda J, et al. NS-018, a selective JAK2 inhibitor, preferentially inhibits CFU-GM colony formation by bone marrow mononuclear cells from high-risk myelodysplastic syndrome patients. Leuk Res. 2014 May;38(5):619-24.

Tamoxifen_Citrate_LCMS_10538_MedChemExpress

=====================================================================Acq. Operator : Yang Qian(LCMS-02) Seq. Line : 7Acq. Instrument : HY-LCMS-02 Location : P1-B-05Injection Date : 10/15/2015 9:18:18 AM Inj : 1Inj Volume : 3.000 µlAcq. Method : D:\AGLIENT 1260\DATA\20151015\20151015 2015-10-15 08-52-17\100-1000MS+3MIN- 1.5_(0.02%FA).MLast changed : 10/15/2015 8:52:17 AM by Yang Qian(LCMS-02)Analysis Method : D:\AGLIENT 1260\DATA\20151012\20151012 2015-10-12 13-29-27\100-1000MS+3MIN- 1.5_(0.02%FA).M (Sequence Method)Last changed : 10/15/2015 1:10:06 PM by Yang Qian(LCMS-02) (modified after loading)Method Info : Postive,MS:100-1000,Column ID:A-RP-132,40℃&DWDORJ 1R +< %DWFK A-RP-134Additional Info : Peak(s) manually integratedmin0.511.522.53mAU 0100200300400500600700 DAD1 B, Sig=214,4 Ref=off (D:\AGLIENT...0\DATA\20151015\20151015 2015-10-15 08-52-17\CPK2015-O14-10538.D)2.1022.1842.470===================================================================== Area Percent Report =====================================================================Sorted By : Signal Multiplier : 1.0000Dilution : 1.0000Do not use Multiplier & Dilution Factor with ISTDsSignal 1: DAD1 B, Sig=214,4 Ref=offPeak RetTime Type Width Area Height Area # [min] [min] [mAU*s] [mAU] %----|-------|----|-------|----------|----------|--------| 1 2.102 MM 0.0391 1.96836 8.38880e-1 0.0470 2 2.184 MM 0.0913 4178.75977 762.85535 99.8669 3 2.470 MM 0.0459 3.60108 1.30880 0.0861Totals : 4184.32920 765.00302===================================================================== *** End of Report ***=====================================================================Acq. Operator : Yang Qian(LCMS-02) Seq. Line : 7Acq. Instrument : HY-LCMS-02 Location : P1-B-05Injection Date : 10/15/2015 9:18:18 AM Inj : 1Inj Volume : 3.000 µlAcq. Method : D:\AGLIENT 1260\DATA\20151015\20151015 2015-10-15 08-52-17\100-1000MS+3MIN- 1.5_(0.02%FA).MLast changed : 10/15/2015 8:52:17 AM by Yang Qian(LCMS-02)Analysis Method : D:\AGLIENT 1260\DATA\20151012\20151012 2015-10-12 13-29-27\100-1000MS+3MIN- 1.5_(0.02%FA).M (Sequence Method)Last changed : 10/15/2015 1:10:53 PM by Yang Qian(LCMS-02) (modified after loading)Method Info : Postive,MS:100-1000,Column ID:A-RP-132,40℃&DWDORJ 1R +< %DWFK A-RP-134Additional Info : Peak(s) manually integratedmin0.511.522.532000004000006000008000001000000 MSD1 TIC, MS File (D:\AGLIENT 1260\DATA\20151015\20151015 2015-10-15 08-52-17\CPK2015-O14-10538.D) ES-API, Pos, S2.198MS Signal: MSD1 TIC, MS File, ES-API, Pos, Scan, Frag: 50 Spectra averaged over upper half of peaks. Noise Cutoff: 1000 counts.Reportable Ion Abundance: > 10%.Retention Mol. Weight Time (MS) MS Area or Ion2.198 ******** 373.30 I 372.30 Im/z10020030040050060070080020406080100*MSD1 SPC, time=2.145:2.291 of D:\AGLIENT 1260\DATA\20151015\20151015 2015-10-15 08-52-17\CPK2015-O14-10538.D ES-API Max: 640220373.3372.3*** End of Report ***。

Tofacitinib_LCMS_10656_MedChemExpress

=====================================================================Acq. Operator : CLJ_701 Seq. Line : 12Acq. Instrument : LCMS-02 Location : P2-D-04Injection Date : 5/15/2017 10:23:16 Inj : 1 Inj Volume : 1.000 µl Acq. Method : D:\CHEM32\1\DATA\20170515\20170515 2017-05-15 09-34-01\1-POS-3MIN.M Last changed : 5/15/2017 9:34:01 by CLJ_701Analysis Method : D:\CHEM32\1\METHODS\1-POS-3MIN.M Last changed : 5/15/2017 11:53:08 by CLJ_701 (modified after loading)Method Info : Mobile Phase: A: water(0.01%TFA) B:ACN(0.01%TFA) Gradient: 5% to 95%B within 1.3 min Flow Rate :1.8ml/min Column :SunFire C18, 4.6*50mm,3.5um A-RP-186 Oven Temperature : 45C Catalog No : HY-40354 Batch#10656 A-RP-186 Additional Info : Peak(s) manually integrated min*0.51 1.52 2.53mAU 0250500750100012501500*DAD1 B, Sig=214,4 Ref=off (D:\CHEM32\1\DATA\20170515\20170515 2017-05-15 09-34-01\CPK2017-515-10656.D)0.589 0.618 1.2971.362 1.613 1.630min*0.51 1.52 2.53mAU 0100200300400*DAD1 C, Sig=254,4 Ref=off (D:\CHEM32\1\DATA\20170515\20170515 2017-05-15 09-34-01\CPK2017-515-10656.D)0.593 1.297 1.630 2.207 ===================================================================== Area Percent Report ===================================================================== Sorted By : Signal Multiplier : 1.0000Dilution : 1.0000Do not use Multiplier & Dilution Factor with ISTDs Signal 1: DAD1 B, Sig=214,4 Ref=off Signal has been modified after loading from rawdata file! Peak RetTime Type Width Area Height Area # [min] [min] [mAU*s] [mAU] %----|-------|----|-------|----------|----------|--------| 1 0.589 MM 0.0127 1.49623 1.96115 0.0721 2 0.618 MM 0.0216 2.27961 1.76002 0.1099 3 1.297 MF 0.0179 2067.12207 1919.35962 99.6184 4 1.362 FM 0.0127 1.25917 1.65057 0.0607 5 1.613 MF 0.0173 1.43455 1.38101 0.0691Peak RetTime Type Width Area Height Area# [min] [min] [mAU*s] [mAU] %----|-------|----|-------|----------|----------|--------|6 1.630 FM 0.0152 1.44920 1.59072 0.0698Totals : 2075.04082 1927.70309Signal 2: DAD1 C, Sig=254,4 Ref=offSignal has been modified after loading from rawdata file!Peak RetTime Type Width Area Height Area# [min] [min] [mAU*s] [mAU] %----|-------|----|-------|----------|----------|--------|1 0.593 MM R 0.0188 1.43692 1.75123 0.29652 1.297 MM R 0.0179 481.81653 455.73083 99.41913 1.630 MM R 0.0362 5.84385e-1 5.14519e-1 0.12064 2.207 MM R 0.0225 7.94062e-1 8.08860e-1 0.1638Totals : 484.63190 458.80544===================================================================== *** End of Report ***=====================================================================Acq. Operator : CLJ_701 Seq. Line : 12Acq. Instrument : LCMS-02 Location : P2-D-04Injection Date : 5/15/2017 10:23:16 Inj : 1 Inj Volume : 1.000 µl Acq. Method : D:\CHEM32\1\DATA\20170515\20170515 2017-05-15 09-34-01\1-POS-3MIN.M Last changed : 5/15/2017 9:34:01 by CLJ_701Analysis Method : D:\CHEM32\1\METHODS\1-POS-3MIN.M Last changed : 5/15/2017 11:53:34 by CLJ_701 (modified after loading)Method Info : Mobile Phase: A: water(0.01%TFA) B:ACN(0.01%TFA) Gradient: 5% to 95%B within 1.3 min Flow Rate :1.8ml/min Column :SunFire C18, 4.6*50mm,3.5um A-RP-186 Oven Temperature : 45C Catalog No : HY-40354 Batch#10656 A-RP-186 Additional Info : Peak(s) manually integratedmin0.51 1.52 2.501000020000300004000050000MSD1 TIC, MS File (D:\CHEM32\1\DATA\20170515\20170515 2017-05-15 09-34-01\CPK2017-515-10656.D) ES-API, Pos, Scan, F 1.330MS Signal: MSD1 TIC, MS File, ES-API, Pos, Scan, Frag: 70 Spectra averaged over upper half of peaks. Noise Cutoff: 1000 counts. Reportable Ion Abundance: > 10%. Retention Mol. Weight Time (MS) MS Area or Ion 1.330 309228 314.15 I 313.15 Im/z 100200300400500600700800020406080100*MSD1 SPC, time=1.303:1.389 of D:\CHEM32\1\DATA\20170515\20170515 2017-05-15 09-34-01\CPK2017-515-10656.D ES-API, PoMax: 35402 314.1 313.1 *** End of Report ***。

Ruxolitinib_S_enantiomer_NP-HPLC_11547_MedChemExpress

===================================================================== *** End of Report ***
Instrument 1 12/08/2016 1:15:55 PM GTT
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Data File D:\CHEM32\1\DATA\20161208\20161208 2016-12-08 09-04-23\L0004-020-004A.D Sample Name:ቤተ መጻሕፍቲ ባይዱL0004-020-004A ===================================================================== Acq. Operator : GTT Seq. Line : 7 Acq. Instrument : Instrument 1 Location : Vial 50 Injection Date : 12/08/2016 12:28:45 PM Inj : 1 Inj Volume : 5.0 µl Acq. Method : D:\CHEM32\1\DATA\20140425\20161208 2016-12-08 09-04-23\OD,80,220NM.M Last changed : 12/08/2016 12:21:41 PM by GTT (modified after loading) Analysis Method : E:\AGILENT\METHODS\OD,65A,220NM.M Last changed : 12/08/2016 1:12:50 PM by GTT (modified after loading) Catalog No : HY-50856A Batch#11547 C-NP-13 Additional Info : Peak(s) manually integrated