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Lactate Assay Kit II 产品说明书
Lactate Assay Kit II Catalog Number MAK065Storage Temperature –20 °CTECHNICAL BULLETINProduct DescriptionL -(+)-Lactate is a metabolic compound formed in animals by the action of the enzyme lactatedehydrogenase. Lactate is produced in proliferating cells and during anaerobic conditions such as exercise. Abnormally high concentrations of lactate have been related to pathological conditions such as cancer, diabetes, and lactate acidosis. L -(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in blood at levels of around 1–2 mmole/L.This kit provides a convenient means for detecting L -(+)-Lactate in biological samples such as in serum, cells, cell culture media, and fermentation media. Lactate concentration is determined by an enzyme assay, which results in a colorimetric (450 nm) product, proportional to the lactate present. There is no need of pretreatment or purification of samples. Typical detection range is 2–10 nmoles of lactate.ComponentsThe kit is sufficient for 100 assays in 96 well ctate Assay Buffer25 mL Catalog Number MAK065A Lactate Enzyme Mix1 vl Catalog Number MAK065B Lactate Substrate Mix1 vl Catalog Number MAK065CL -(+)-Lactate Standard, 100 mM0.1 mLCatalog Number MAK065DReagents and Equipment Required but Not Provided.• 96 well flat-bottom plate –It is recommended to useclear plates for colorimetric assays.• Spectrophotometric multiwell plate reader.• 10 kDa Molecular Weight Cut-Off (MWCO) SpinFilter (optional for serum-containing samples).Precautions and DisclaimerThis product is for R&D use only, not for drug,household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.Preparation InstructionsBriefly centrifuge vials before opening. Use ultrapure water for the preparation of reagents. To maintain reagent integrity, avoid repeated freeze/thaw ctate Assay Buffer –Allow buffer to come to roomtemperature before ctate Enzyme Mix –Reconstitute in 220 µL ofLactate Assay Buffer. Mix well by pipetting, then aliquot and store at –20 °C. Use within 2 months of reconstitution. Lactate Substrate Mix –Reconstitute in 220 µL ofLactate Assay Buffer. Mix well by pipetting, andstore at 4 °C. Use within 2 months of reconstitution. Storage/StabilityThe kit is shipped on wet ice and storage at –20 °C, protected from light, is recommended.2ProcedureAll samples and standards should be run in duplicate. Lactate Standards for Colorimetric DetectionDilute 10 µL of the 100 mM Lactate Standard with990 µL of the Lactate Assay Buffer to prepare a 1 mM standard solution. Add 0, 2, 4, 6, 8, 10 µL of the 1 mM standard solution into a 96 well plate, generating 0 (blank), 2, 4, 6, 8, and 10 nmole/well standards. Add Lactate Assay Buffer to each well to bring the volume to 50 µL.Sample PreparationColorimetric assays require 50 µL of sample for each reaction (well).Tissue or cells can be homogenized in 4 volumes of the Lactate Assay Buffer. Centrifuge the samples at13,000 ×g for 10 minutes to remove insoluble material. Samples should be deproteinized with a 10 kDa MWCO spin filter to remove lactate dehydrogenase. The soluble fraction may be assayed directly.Serum samples (0.5–10 µL/assay) can be assayed directly by adding in duplicate to 96-well plate. If lactate dehydrogenase activity is present, samples should be deproteinized with a 10 kDa MWCO spin filter.Bring samples to final volume of 50 µL/well with Lactate Assay Buffer.For unknown samples, it is suggested to test several sample volumes to make sure the readings are within the standard curve range.Note: Lactate Dehydrogenase (LDH) will degrade lactate. Samples containing LDH (such as culture medium or tissue lysate) should be kept –80 °C for storage, and filtered through a 10 kDa cut-off spin filter. Complete medium containing FBS should be deproteinized due to high LDH content.Assay Reaction1.Set up the appropriate Reaction Mixes according tothe scheme in Table 1. 50 µL of the appropriateReaction Mix is required for each reaction (well).Note: NADH or NADPH from cell or tissue extracts generates background for the lactate assay. Toremove the effect of NADH or NADPH background,a blank sample may be set up for each sample byomitting the Lactate Enzyme Mix. The blankreadings can then be subtracted from the samplereadings.Table 1.Reaction Mixes2.Add 50 µL of the appropriate Reaction Mix to eachof the wells. Mix well using a horizontal shaker orby pipetting, and incubate the reaction for30 minutes at room temperature. Protect the platefrom light during the incubation.3.Measure the absorbance at 450 nm (A450).The coloris stable for up to 4 hours.3 ResultsCalculationsThe background for the assay is the value obtained for the 0 (blank) lactate standard. Correct for the background by subtracting the blank value from all readings. Background values can be significant and must be subtracted from all readings. Use the values obtained from the appropriate lactate standards to plot a standard curve.Subtract the blank sample readings from the sample readings. The amount of lactate present in the samples may be determined from the standard curve.Note: A new standard curve must be set up each time the assay is run.Concentration of LactateS a/S v= CS a= Amount of lactate acid in unknown sample (nmole) from standard curveS v= Sample volume (µL) added into the wells.C = Concentration of lactate acid in sampleLactate molecular weight: 89.07 g/moleSample CalculationAmount of Lactate (S a) = 5.07 nmoleSample volume (S v) = 50 µLConcentration of lactate in sample5.07 nmole/50 µL = 0.101nmole/µL0.101 nmole/µL ×89.07 ng/nmole= 9.0 ng/µL4LS,MF,MAM 03/12-1©2012 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich Co. LLC, registered in the US and other countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at and/or on the reverse side of the invoice or packing slip.。
毕赤酵母表达知识归纳
毕赤酵母表达知识归纳1a.配制500×BIOTIN stock solution（0.02％）有这么3种方案：1、懒人是将Biotin直接溶在去离子水中，放过夜，基本就能溶；2、急性子是将溶液配成0.02N的NaOH，就很容易溶解了；3、水浴加热，温度不能高于50度。
D－生物素是具有生物活性的生物素，也就是vitaminH。
在毕赤酵母代谢过程中，作为多种酶的辅基起作用。
天然培养基中一般可以不单独添加，因为YNB中、酵母粉、蛋白胨中均含有一定量的生物素，但是做高密度发酵还是必须要添加的。
b.有几个比较迷惑的问题请教大家：(很典型的小问题）1、制感受态细胞，OD多少比较好？pyrimidine 战友的方法：取1mlGS115过夜培养物(OD约6-10) 分装到1.5ml EP管中。
说明书还有一些文献是说在1.3左右效率高，再高了效率会很低2、关于高效转化法，文献说用(LiAc)，而invitrogen的说明书说转化毕赤酵母用(LiAc)没用，要用LiCl。
Lithium acetate does not work with Pichia pastoris. Use only lithium chloride.3、YNB到底能高温灭么？有的说能有的说不能。
过滤灭菌的怎么操作？我是把滤器装好膜绑到瓶口用纱布盖上，报纸包上，瓶盖放烧杯里单灭。
然后把配好的溶液用注射器一点点推进去。
4、葡萄糖为什么在YPD里一起灭颜色很深，单灭则不会。
该115度还是121度灭？网上搜了下，都有人用！5、电转化参数用400欧还是200欧？有的用400，有的还专门说不是用400。
都是从园里看到的！电击参数：1.5KV，25uF，200欧姆（不是400）6、电转后，在MD平板上长的应该就是整合了目的基因的重组子了吧？如果不想筛高拷贝的，是否PCR验证一下即可？网友的回答：ynb最好不灭菌，我是0.22um过滤处理的。
invitrogen手册上可以灭菌的。
CelLyticTM系列蛋白裂解液
高效率 操作步骤简单,省时 高得率 得率远优于传统冻融或超声法 高活性 温和非变性条件下抽提活性蛋白 高兼容 与蛋白酶抑制剂、鳌合剂、离液剂等很好兼容 抽提的蛋白无需去除CelLyticTM 试剂即可进行下游实验： 亲和纯化 Western blot 凝胶迁移检测 报告基因检测……
566.28 2136.42 566.28 3758.04 2535.39 3938.22
501.93 3320.46
566.28 2084.94 3989.7
527.67 3629.34 5302.44
促销价¥
384.81 2375.80 685.97 2375.80 409.91 1505.79 2651.86 1054.05 1648.00 393.18 1187.90 217.50 711.07 4341.69 368.08 1271.56
哺乳动物细胞和组织裂解
C2978
CelLytic™ M Cell Lysis Reagent
C3228
CelLytic™ MT Cell Lysis Reagent
CE0500 NXTRACT
R0278
CelLytic™ MEM Protein Extraction Kit CelLytic™ NuCLEAR™ Extraction Kit For mammalian tissue or cultured cells RIPA Buffer
P8215
Protease Inhibitor Cocktail for use with fungal and yeast extracts
P8465
Protease Inhibitor Cocktail for use with bacterial cell extracts
MRS培养基(莫匹罗星锂盐和半胱氨酸盐酸盐改良MRS培养基基础)说明书
广东环凯微生物科技有限公司网址： 地址：广州市黄埔区科学城神舟路788号邮编：510663传真：860288778876产品说明书Product Manual【产品名称】通用名称：MRS 培养基（莫匹罗星锂盐和半胱氨酸盐酸盐改良MRS 培养基基础）英文名称：MRS Medium(Li-Mupirocin and Cysteine Hydrochloride Modified MRS Medium Base)【产品编号与包装规格】产品编号产品类型包装规格027315干粉250g/瓶【产品用途】用于乳酸菌的分离、计数和培养。
【检验原理】蛋白胨、牛肉膏粉、酵母膏粉提供氮源、维生素、生长因子;葡萄糖为可发酵糖类;磷酸氢二钾为酸碱缓冲剂;柠檬酸三铵、硫酸镁、硫酸锰、吐温-80和乙酸钠为培养各种乳酸菌提供生长因子，其成分还能抑制某些杂菌;琼脂是培养基的凝固剂。
【使用方法】称取本品64.3g，加入蒸馏水或去离子水1L，搅拌加热煮沸至完全溶解，121℃高压灭菌15min。
如需配置莫匹罗星锂盐和半胱氨酸盐酸盐改良MRS 培养基，待培养基冷却至45℃，每100mLMRS 培养基添加配套试剂SR0370莫匹罗星锂盐和半胱氨酸盐酸配套试剂各1支，混合均匀。
倾注无菌平皿，备用。
【质量控制】1、下列质控菌株接种于MRS 培养基上，在35～37℃培养48±2h ，观察结果如下表：2、下列质控菌株接种于莫匹罗星锂盐和半胱氨酸盐酸盐改良MRS培养基上，在35～37℃培养48±2h，观察结果如下表：贮存于避光、干燥处，用后立即旋紧瓶盖；贮存期三年。
【注意事项】1、称量时注意粉尘，佩戴口罩操作以避免引起呼吸道系统不适。
2、干粉培养基使用后立即旋紧瓶盖，避免吸潮结块。
贮存于避光、干燥处。
未开封产品保质期三年。
开封后根据存放条件的不同保质时间存在一定的差异。
3、质检报告可以登录环凯网站，打开“质检报告”页面，输入产品批号下载。
化妆品原料商品名与化学名对应
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Buriti Crodamol MLCrodamol MMCropure Evening Primrose Crodasinic OVolpo N10Crodafos N10AVolpo N2Volpo N20Volpo N3Crodafos N3AVolpo N5Crodafos N5ANovolCrodamazon Babassu Cropure Babassu Crodacid PD03161Crodet S100Cithrol 6MSEtocas 15Glycerox L15CrothixCrothix LiquidCithrol DEGMS N/ECithrol 10MSEtocas 29Incroquat HO-80PGEtocas 35Cithrol 2DOEtocas 40Croduret 40Crodet S40Croduret 50 Special Crovol BA70GCrovol PK70Etocas 5Glycerox 767Crovol A70Crodasol ACCrovol M70Crovol EP70Croduret 60Glycerox HECroduret 7 Special SolanECithrol 4DOGlycerox L8Cithrol 4MLCithrol 4MSSupermol LSupermol SCrodamol PTISCrester PRCrillet 1 SuperCrillet 2 SuperCrillet 3 SuperCrillet 4 SuperCrillet 45Procetyl 10Probutyl 14Prostearyl 15Crodamol PMPProcas H3Promyristyl PM3Crodamol PCCrodasinic LS95Adinol CT95Crill 6Crill 1Crill 4Crill 2Crill 43Crill 3Crill 45Crill 41Incromine SBVolpo S2Volpo S20Cromul EM1207Crodacol S95EPCrodesta F-10Crodaderm BCrodaderm SCrodesta F-160Syncrowax BB4Syncrowax HRC聚季铵盐-44十三烷醇聚醚硫酸钠PEG-80 失水山梨醇月桂酸酯PEG-10 聚二甲基硅氧烷PEG-120 二硬脂酸酯月桂醇聚醚-4苯氧乙醇乙酰胺 MEA禾大乙酰胺 MEA/乳酰胺 MEA禾大乙酰化羊毛脂禾大乙酰化羊毛脂禾大野杏仁氨基酸类禾大西曲氯铵禾大椰油酰胺丙基甜菜碱禾大椰油基二甲基铵羟丙基水解胶原禾大椰油基二甲基铵羟丙基水解角蛋白禾大椰油基二甲基铵羟丙基水解小麦角蛋白禾大椰油基二甲基铵羟丙基水解丝氨基酸类禾大胶原氨基酸类禾大胶原氨基酸类禾大胱氨酸双-PG-丙基硅烷三醇禾大水解胶原禾大水解弹性蛋白禾大水解角蛋白禾大水解角蛋白禾大水解角蛋白禾大水解角蛋白禾大水解燕麦禾大水解米糠蛋白禾大水解蚕丝禾大水解蚕丝禾大水解大豆蛋白禾大水解植物蛋白禾大水解小麦蛋白禾大水解小麦蛋白禾大水解小麦蛋白禾大水解小麦蛋白/水解小麦淀粉禾大水解小麦蛋白 PG-丙基硅烷三醇禾大水解小麦蛋白/PVP 交联聚合物禾大羟丙基三甲基氯化铵水解小麦蛋白禾大角蛋白/水解角蛋白禾大角蛋白氨基酸禾大月桂基二甲基铵羟丙基水解胶原禾大月桂基二甲基铵羟丙基水解角蛋白禾大月桂基二甲基铵羟丙基水解小麦蛋白禾大月桂基二甲基铵羟丙基水解小麦蛋白/月桂基二甲基铵羟丙基水解小麦淀粉禾大月桂基二甲基铵羟丙基水解大豆蛋白禾大PEG-60 月见草油甘油酯类禾大PG-羟乙基纤维素椰油基二甲基氯化铵禾大PG-羟乙基纤维素月桂基二甲基氯化铵禾大PG-羟乙基纤维素硬脂基二甲基氯化铵禾大月桂酰小麦氨基酸钾禾大蚕丝氨基酸禾大月桂酰肌氨酸钠禾大月桂酰肌氨酸钠禾大甲基椰油酰基牛磺酸钠禾大肉豆蔻酰肌氨酸钠禾大可溶胶原禾大可溶胶原禾大司拉氯铵/异丙醇/鲸蜡硬脂醇禾大硬脂酰胺丙基二甲胺乳酸盐禾大硬脂基二甲基铵羟丙基水解胶原禾大硬脂基二甲基铵羟丙基水解小麦蛋白禾大蔗糖椰油酸酯/乙醇禾大月桂酰基胶原氨基酸类TEA盐禾大小麦氨基酸禾大水解植物蛋白 PG-丙基硅烷三醇禾大木鲁星果棕（ASTROCARYUM MURUMURU）脂禾大山嵛基苄基二甲基氯化铵/鲸蜡醇禾大山嵛酸禾大山嵛基三甲基氯化铵/异丙醇禾大山嵛基三甲基铵甲基硫酸盐/鲸蜡醇/丁二醇禾大巴西果（BERTHOLLETIA EXCELSA）提取物禾大玻璃苣（BORAGO OFFICINALIS）禾大硬脂酸丁酯禾大C10-30 酸胆甾醇/羊毛甾醇混合酯禾大C12-13 链烷醇聚醚-23禾大C12-13 链烷醇聚醚-3禾大C12-13 链烷醇聚醚-4禾大C12-15 醇苯甲酸酯禾大C18-36 酸甘醇酯禾大C18-36 酸甘油三酯禾大辛酸/癸酸甘油三酯禾大苦油树（CARAPA GUAIANENSIS）籽油禾大红花（CARTHAMUS TINCTORIUS）籽油禾大无数据禾大鲸蜡硬脂醇聚醚-12禾大鲸蜡硬脂醇聚醚-2 磷酸酯禾大鲸蜡硬脂醇聚醚-20禾大鲸蜡硬脂醇聚醚-25禾大鲸蜡硬脂醇聚醚-6禾大鲸蜡硬脂醇禾大鲸蜡硬脂醇禾大山嵛基苄基二甲基氯化铵/鲸蜡硬脂醇禾大鲸蜡硬脂醇聚醚-20/鲸蜡硬脂醇禾大鲸蜡硬脂醇聚醚-20/鲸蜡硬脂醇禾大鲸蜡硬脂醇聚醚-20/鲸蜡硬脂醇禾大鲸蜡醇聚醚-20 磷酸酯/二鲸蜡醇磷酸酯/鲸蜡硬脂醇禾大鲸蜡醇聚醚-10 磷酸酯/二鲸蜡醇磷酸酯/鲸蜡硬脂醇禾大无数据禾大鲸蜡醇聚醚-10 磷酸酯/鲸蜡硬脂醇聚醚-20/鲸蜡硬脂醇禾大鲸蜡醇聚醚-5/鲸蜡硬脂醇聚醚-7禾大鲸蜡醇禾大鲸蜡醇/山嵛基三甲基铵甲基硫酸盐/季铵盐-33禾大合成鲸蜡禾大鲸蜡醇棕榈酸酯禾大鲸蜡醇磷酸酯禾大胆甾醇禾大椰油酰肌氨酸禾大二鲸蜡基二甲基氯化铵/异丙醇禾大己二酸二乙基己酯禾大琥珀酸二乙基己酯禾大己二酸二异丁酯禾大己二酸二异丙酯禾大二PPG-2 肉豆蔻油醇聚醚-10 己二酸酯禾大二PPG-3 肉豆蔻基醚己二酸酯禾大油酸乙酯禾大椰油酸乙基己酯禾大羟基硬脂酸乙基己酯禾大棕榈酸乙基己酯禾大壬酸乙基己酯禾大硬脂酸乙基己酯禾大甘油聚醚-26禾大甘油聚醚-26 磷酸酯禾大甘油禾大甘油油酸酯禾大甘油硬脂酸酯禾大甘油硬脂酸酯/PEG-100 硬脂酸酯禾大甘油硬脂酸酯 SE禾大乙二醇二硬脂酸酯禾大乙二醇硬脂酸酯禾大乙二醇硬脂酸酯/硬脂酰胺 AMP禾大氢化羊毛脂禾大水解胶原禾大水解胶原禾大水解胶原禾大水解胶原禾大水解角蛋白禾大水解牛奶蛋白禾大水解燕麦禾大羟丙基三甲基氯化铵水解胶原禾大羟丙基三甲基氯化铵水解胶原禾大羟丙基三甲基氯化铵水解胶原/羟丙基三甲基氯化铵水解小麦淀粉禾大异鲸蜡醇硬脂酸酯禾大羊毛脂酸异丙酯禾大肉豆蔻酸异丙酯禾大棕榈酸异丙酯禾大异十三醇异壬酸醇禾大乳酰胺 MEA禾大羊毛脂醇聚醚-n禾大羊毛脂禾大羊毛脂禾大羊毛脂禾大羊毛脂酸禾大羊毛脂醇禾大羊毛脂蜡禾大羊毛脂油禾大月桂酰肌氨酸禾大月桂醇乳酸酯禾大白池花（LIMNANTHES ALBA）籽油禾大毛瑞榈（MAURITIA FLEXUOSA）提取物禾大肉豆蔻醇乳酸酯禾大肉豆蔻醇肉豆蔻酸酯禾大月见草（OENOTHERA BIENNIS）油禾大油酰肌氨酸禾大油醇聚醚-10禾大油醇聚醚-10 磷酸酯禾大油醇聚醚-2禾大油醇聚醚-20禾大油醇聚醚-3禾大油醇聚醚-3 磷酸酯禾大油醇聚醚-5禾大油醇聚醚-5 磷酸酯禾大油醇禾大巴巴苏（ORBIGNYA OLEIFERA）籽油禾大巴巴苏（ORBIGNYA OLEIFERA）籽油禾大棕榈酸禾大PEG-100 硬脂酸酯禾大PEG-12 硬脂酸酯禾大PEG-15 蓖麻油禾大PEG-15 甘油月桂酸酯禾大PEG-150 季戊四醇四硬脂酸酯禾大PEG-150 季戊四醇四硬脂酸酯/PEG-6 辛酸/癸酸甘油酯类禾大PEG-2 硬脂酸酯禾大PEG-20 硬脂酸酯禾大PEG-29 蓖麻油禾大PEG-3 二油酰胺乙基甲基铵甲基硫酸盐/丙二醇禾大PEG-35 蓖麻油禾大PEG-4 二油酸酯禾大PEG-40 蓖麻油禾大PEG-40氢化蓖麻油禾大PEG-40 硬脂酸酯禾大PEG-45氢化蓖麻油/PEG-45氢化蓖麻油禾大PEG-42 巴巴苏甘油酯类禾大PEG-45 棕榈仁油甘油酯类禾大PEG-5 蓖麻油禾大PEG-6 辛酸/癸酸甘油酯类禾大PEG-60 杏仁甘油酯类禾大PEG-60 杏仁甘油酯类/禾大PEG-60 玉米油甘油酯类禾大PEG-60 月见草油甘油酯类禾大PEG-60氢化蓖麻油禾大PEG-7 甘油椰油酸酯禾大PEG-7氢化蓖麻油禾大PEG-75 羊毛脂禾大PEG-8 二油酸酯禾大PEG-8 甘油月桂酸酯禾大PEG-8 月桂酸酯禾大PEG-8 硬脂酸酯禾大季戊四醇异硬脂酸酯/癸酸酯/辛酸酯/己二酸酯禾大季戊四醇硬脂酸酯/癸酸酯/辛酸酯/己二酸酯禾大季戊四醇四异硬脂酸酯禾大聚甘油-3 聚蓖麻醇酸酯禾大聚山梨醇酯-20禾大聚山梨醇酯-40禾大聚山梨醇酯-60禾大聚山梨醇酯-80禾大聚山梨醇酯-85禾大PPG-10 鲸蜡基醚禾大PPG-14 丁醚禾大PPG-15 硬脂醇醚禾大PPG-2 肉豆蔻醇醚丙酸酯禾大PPG-3 氢化蓖麻油禾大PPG-3 肉豆蔻醇醚禾大丙二醇二辛酸酯/二癸酸酯禾大月桂酰肌氨酸钠禾大甲基椰油酰基牛磺酸钠禾大山梨坦异硬脂酸酯禾大山梨坦月桂酸酯禾大山梨坦油酸酯禾大山梨坦棕榈酸酯禾大山梨坦倍半油酸酯禾大山梨坦硬脂酸酯禾大山梨坦三油酸酯禾大山梨坦三硬脂酸酯禾大硬脂酰胺丙基二甲胺禾大硬脂醇聚醚-2禾大硬脂醇聚醚-20禾大硬脂醇聚醚-21禾大硬脂醇禾大蔗糖二硬脂酸酯禾大蔗糖多山嵛酸酯禾大蔗糖多大豆油酸酯禾大蔗糖硬脂酸酯禾大合成蜂蜡禾大三山嵛精禾大聚季铵盐-44十三烷醇聚醚硫酸钠PEG-80 失水山梨醇月桂酸酯PEG-10 聚二甲基硅氧烷PEG-120 二硬脂酸酯月桂醇聚醚-4苯氧乙醇。
MC1061感受态细胞使用说明书
MC1061感受态细胞基因组：写法1 araD139, Δ(ara, leu)7697, ΔlacX74, galU-, galK-, hsr-, hsm+, strA写法2 F−ΔlacX74 mcrB1 e14−(mcrA0) rpsL150(Str R) galE15 galK16 Δ(ara, leu)7697 araΔ139λ−hsdR2(r k−, m k+)spoT1写法3 F- araD139 (ara-leu)7696 galE15 galK16 (lac)X74 rpsL (Str r) hsdR2 (r k-m k+) mcrA mcrB1产品简介：good host for electroporation; defective for EcoK restriction butmodification-proficient; defective for McrA and McrBC restriction systems; Lac–(operon deleted, but I’m not sure whether lacI gene is functional); can’t metabolize arabinose; can’t metabolize galactose; streptomycin resistant; requires thiamine操作说明：1，收到本产品请直接冻存于-80℃冰箱备用。
2，取冻存于-80度冰箱的100μl感受态细胞于冰上融化。
3，加入1μl纯质粒或10ul连接产物，轻轻吹打混匀，冰浴30 min。
4，将菌液放入42℃水浴中热激90秒，立即放入冰浴中5 min。
5，加入800ul LB，于37℃恒温摇床上150rpm×45min温育。
6，将菌液4000rpm/min离心3min，留200μl上清将菌体打散，均匀涂布于含适当浓度的相应抗生素的琼脂平板表面，平板于37℃可先正放置1h,待液体吸收完毕，再倒置培养过夜。
Hyclone液体培养基大全 (查成分,货号,包装必看)
Ｈyｃlone液体培养基大全,由厚百商城整理编辑。
Ｈycloｎe培养基产品丰富,面对长长得培养基列表，同一个名称您能知道哪个就就是您在找得吗?这里厚百商城就为您列出各培养基及所含得主要成分,一眼瞧出区别,帮助您快速选择出在找得那瓶培养基。
货号标记说明：
“●”表示常规生产产品。
“○”表示按客户订单生产产品,有一定得最小定制量并需要一定时间。
“□”表示未储存类项目，需要一段时间供给。
货号后标有“B”得为赛默飞世尔生物化学制品(北京)有限公司生产,其她得为美国原产。
厚百商城提醒您:
所有液体培养基均经１00纳米无菌过滤与出厂严格检测,除非需要,请不要私自进行二次过滤与任何形式得无菌检测。
巴斯夫非离子表面活性剂luten...
巴斯夫⾮离⼦表⾯活性剂luten...详细介绍：⾮离⼦表⾯活性剂产品⽬录浊点活性物HLB应⽤产品名称化学结构Lutensol®A3N C12-14-51-54[E]1008.5乳化，中间体脂肪醇+ 3EOLutensol®A7N+ 7EO53-59[A]10012洗涤，乳化，脱脂Lutensol®A9N+ 9EO73-77[A]10013.5洗涤，乳化Lutensol®XP 30C10-脂30-33[E]1009乳化，中间体肪醇+ 3EOLutensol®XP 50+ 5EO54-57[E]10011.5乳化，中间体Lutensol®XP 70+ 7EO67-70[E]10013润湿，脱脂Lutensol®XP 79+ 7EO67-70[E]8513润湿，脱脂Lutensol®XP 80+ 8EO54-57[A]10014润湿，脱脂，洗涤Lutensol®XP 89+ 8EO54-57[A]8514润湿，脱脂，洗涤Lutensol®XP 90+ 9EO67-70[A]10014.5洗涤，脱脂Lutensol®TO 3C13-脂38-42[E]1009乳化，中间体肪醇+ 3EOLutensol®TO 5+ 5EO60-62[E]10010.5乳化，中间体Lutensol®TO 6+ 6EO69-72[D]10011乳化，脱脂Lutensol®TO 7+ 7EO69-71[E]10012乳化，脱脂，洗涤，润湿Lutensol®TO 8+ 8EO58-62[A]10013乳化，脱脂，洗涤，润湿Lutensol®TO 89+ 8EO58-62[A]9013乳化，脱脂，洗涤，润湿Lutensol®TO 12+ 12EO73-77[B]10014.5乳化，分散Lutensol®XL 40C13-脂45-48[E]10010.5乳化肪醇+ 4EOLutensol®XL 50+ 5EO58-61[E]10011.5乳化，润湿Lutensol®XL 60+ 6EO68-71[E]10012.5乳化，润湿，洗涤Lutensol®XL 70+ 7EO67-70[E]10012.5乳化，润湿，洗涤，脱脂Lutensol®XL 80+ 8EO54-57[A]10013乳化，润湿，洗涤，脱脂Lutensol®XL 90+ 9EO67-70[A]10014乳化，润湿，洗涤，脱脂Plurafac®LF 90037-41[E]100低泡润湿Plurafac®LF 90136-40[A]100低泡润湿Emulan®TO 2080C13-脂肪醇+ 20E8016乳化，分散，匀化OEmulan®TO 3070+ 30EO7017乳化，分散，匀化Emulan®TO 40+ 40EO10018乳化，分散，匀化Emulan®TO 4070+ 40EO7018乳化，分散，匀化Emulan®LVA85乳化剂Emulan®TXI100乳化剂Emulan®TXO100乳化剂PluronicPE610021-25[A]100乳化，低泡洗涤，分散，抑泡PluronicPE620031-35[A]100乳化，低泡洗涤，分散，抑泡PluronicPE640059-61[A]100乳化，低泡洗涤，分散PluronicPE810035-37[E]100乳化，低泡洗涤，分散，抑泡Pluriol®E 200分⼦量200100溶剂，中间体，保湿，润湿Pluriol®E 300分⼦量300100溶剂，中间体，保湿，润湿Pluriol®E 400分⼦量400100溶剂，中间体，保湿，起泡，润湿Pluriol®E 600分⼦量600100溶剂，中间体，保湿，起泡，分散Pluriol®E 1000分⼦量1000100溶剂，保湿，分散浊点测试⽅法（EN 1890）：[A]: 1g表⾯活性剂在100g去离⼦⽔中[B]: 1g表⾯活性剂在100g氯化钠溶液中（浓度为50g/l）[C]: 1g表⾯活性剂在100g氯化钠溶液中（浓度为100g/l)[D]: 5g表⾯活性剂在45g⼆⼄⼆醇丁醚溶液中（浓度为250g/l）[E]: 5g表⾯活性剂在25g⼆⼄⼆醇丁醚溶液中（浓度为250g/l）低泡环保乳化剂产品应⽤说明：产品技术参数：* EO=⼄氧基** 如果没有特别注明，产品含量为100%品名化学名⼄氧基含量Synative/Pluronic RPE1720(17R2)PO-EO 嵌段共聚物20%EOSynative/Pluronic RPE1740 (17R4)PO-EO 嵌段共聚物40%EOSynative/Pluronic RPE2520 (25R2)PO-EO 嵌段共聚物20%EOPluronic RPE2525PO-EO 嵌段共聚物25%EOPluronic RPE3110PO-EO 嵌段共聚物10%EOPluronic PE3100EO-PO 嵌段共聚物10%EOPluronic PE4200EO-PO 嵌段共聚物20%EOPluronic PE4300EO-PO 嵌段共聚物30%EOPluronic PE6100EO-PO 嵌段共聚物10%EOPluronic PE6200EO-PO 嵌段共聚物20%EOPluronic PE6400EO-PO 嵌段共聚物40%EOPluronic PE6800EO-PO 嵌段共聚物80%EOPluronic PE8100EO-PO 嵌段共聚物10%EOPluronic PE9200EO-PO 嵌段共聚物20%EOPluronic PE10100EO-PO 嵌段共聚物10%EOPluronic PE10300EO-PO 嵌段共聚物30%EOPluronic PE10400EO-PO 嵌段共聚物40%EOPluronic PE10500EO-PO 嵌段共聚物50%EOPluronic PE10500EO-PO 嵌段共聚物50%EOPluronic F108,F108NF EO-PO 嵌段共聚物80%EOPluronic L121EO-PO 嵌段共聚物10%EOPluronic P123EO-PO 嵌段共聚物30%EOPluronic F127,F127NF EO-PO 嵌段共聚物70%EOSynative X300EO-PO 嵌段共聚物Synative X310EO-PO 嵌段共聚物Synative X320EO-PO 嵌段共聚物Tetronic 904EO-PO-⼄烯基⼆元胺共聚物40%EOTetronic 1107EO-PO-⼄烯基⼆元胺共聚物70%EOPlurafac LF 901/Emulan TXI脂肪醇烷氧基化合物Plurafac LF 900/Emulan TXO脂肪醇烷氧基化合物Plurafac LF 120脂肪醇烷氧基化合物95%Plurafac LF 220脂肪醇烷氧基化合物95%Plurafac LF 221脂肪醇烷氧基化合物98%Plurafac LF 223脂肪醇烷氧基化合物Plurafac LF 224脂肪醇烷氧基化合物Plurafac LF 300脂肪醇烷氧基化合物Plurafac LF 305脂肪醇烷氧基化合物Plurafac LF 400脂肪醇烷氧基化合物Plurafac LF 401脂肪醇烷氧基化合物Plurafac LF 403脂肪醇烷氧基化合物Plurafac LF 404脂肪醇烷氧基化合物Plurafac LF 405脂肪醇烷氧基化合物95%Plurafac LF 500脂肪醇烷氧基化合物Plurafac LF 600脂肪醇烷氧基化合物Plurafac LF 700脂肪醇烷氧基化合物Plurafac LF 711脂肪醇烷氧基化合物Plurafac LF 1300脂肪醇烷氧基化合物Plurafac LF 031脂肪醇烷氧基化合物95%Plurafac LF 131耐碱脂肪醇烷氧基化合物Plurafac LF 132耐碱脂肪醇烷氧基化合物Plurafac LF 231耐碱脂肪醇烷氧基化合物Plurafac LF 431耐碱脂肪醇烷氧基化合物Plurafac LF 1430特种烷氧基化合物Plurafac SLF-18B4590% Synative AC PWM 2脂肪醇⼄氧基化合物Synative AC LS 4 L⽉桂醇⼄氧基化合物Dehypon LS 24脂肪醇C12-14+2EO+4PODehypon LS 36脂肪醇C12-14+3EO+6PODehypon LS 45脂肪醇C12-14+4EO+5PODehypon LS 54脂肪醇C12-14+5EO+4PODehypon 2574脂肪醇C12-14+6EO+8PODehypon G 162格尔伯特醇C16+2EODehypon OCP 502油醇/鲸蜡醇烷氧基化合物。
氰特湛新氨基树脂资料 AllnexAminosProductGuide
AMINO CROSSLINKERSPRODUCT GUIDE • CYMEL® Resins •WorldwideAll About Resins FACTS & FIGURESAbout usdimately3Amino ResinsAllnex's expertise in crosslinking technology is part of our corporate heritage and the foundation to our leadership in supplying the market for amino resins. This brochureprovides product characteristics, compositional information and brief general comments on CYMEL ® amino crosslinkers used in industrial coatings applications includingautomotive, Original Equipment Manufacturers (OEM),kitchen cabinets, metal food and beverage packaging, metal building products and general metal finishing.Our new CYMEL formaldehyde-free crosslinkers are ideal for applications where formaldehyde emissions are a concern.Amino crosslinkers are used with epoxy, polyester, acrylic and alkyd resins and provide the desired balance offlexibility, exterior durability, chemical resistance and film toughness.Allnex's Goals in Research and DevelopmentSeveral factors drive Allnex's research and development efforts. Allnex focuses on gaining a fundamentalunderstanding of the technical challenges encountered by our customers as they work towards improving their formulations. Allnex also focuses on offering solutions quickly and cost-effectively. Equally important is ourcommitment to developing new products that fulfill long-standing needs of the industries we serve. Our technical specialists routinely visit customer locations, worldwide, to assist them in resolving problems and accelerating development of better products.True Customer CommitmentWith our extensive portfolio of liquid resin & additive, radiation cured and powder coating resin & additive, and crosslinker technologies, we are ideally positioned to help customers find the solutions to all of their coating challenges. We are dedicated to delivering value through the development of innovative, market-leading, high quality products that offer enhanced performance, increased ease-of-use, environmental compliance and reduced cost.IntroductionHigh Solids Methylated Melamine Resins ...............4Highly Methylated Melamine Resins ....................... 4Methylated High Imino Melamine Resins ................5Partially Methylated Melamine Resins .. (5)High Solids Mixed Ether Melamine Resins ............. 6 Highly Alkylated Melamine Resins ........................... 6High Imino Melamine Resins (7)Butylated Melamine Resins ....................................... 8n-Butlyated Melamine Resins ................................... 8Highly n-Butylated Melamine Resins ........................8n-Butylated High Imino Melamine Resins .............. 9iso-Butylated Melamine Resins (9)High Solids Urea Resins ........................................... 10Methylated Resins .................................................... 10Butylated Resins . (10)Butylated Urea Resins .............................................. 12n-Butylated Urea Resins ........................................... 12iso-Butylated Urea Resins . (13)Benzoguanamine and Glycoluril Resins ............... 14Benzoguanamine Resins ......................................... 14Glycoluril Resins .. (15)Formaldehyde Free Resins ...................................... 16CYMEL NF 2000 ........................................................ 16CYMEL NF 3041 .. (16)Table of ContentsHighly Methylated Melamine ResinsHighly methylated melamine resins consist of commercial versions of hexamethoxymethylmelamine (HMMM). They differ primarily in their degree of alkylation and monomer content. All are efficient crosslinking agentsfor hydroxyl, carboxyl and amide functional polymers. The practical equivalent weight for most of the resins is 130-180. Advantages are low VOCs; high film flexibility and toughness when used with inherently flexible backbone resins; excellent formulation stability, especially in waterborne systems formulated at a pH of 8-9; good mar resistance; and good intercoat adhesion properties. As typical with melamine resins, all are low in color and color development, have excellent exterior durability and good heat resistance. Because of their high extent of alkylation, the resins in this series require the addition of a strong acid catalyst for acceptable cure response when bakedat 125-150°C. Typically, 0.2- 0.4% p-toluene sulfonic acid based on total binder solids is recommended. The optimum concentration of acid catalyst depends on the basicity of the other components in the formulation and should be determined experimentally. Using a blocking amine forthe acid catalyst and adding a stabilizing alcohol to the formulation can enhance formulation stability.High Solids Methylated Melamine Resins4Methylated High Imino Melamine ResinsMethylated high imino melamine resins are partially methylolated and highly alkylated. These characteristics result in resins containing a significant concentration of alkoxy/imino or high NH functionality. The advantagesare fast cure response at 120-150°C without the need for strong acid catalysts, fast cure response in waterborne formulations, high film hardness, and low formaldehyde release on cure. In addition to reacting with hydroxyl, carboxyl and amide functionality on polymers, these resins also self-condense readily. Therefore, their practical equivalent weight is typically 180-240. They too, can be stabilized by adding tertiary amine and stabilizing primary alcohol to the formulation. Compared to their highly alkylated counterparts, the imino resins usually result in slightly less flexible coatings and slightly higher VOCs when used in solvent-based systems.Partially Methylated Melamine ResinsPartially methylated melamine resins are highly methylolated and partially alkylated. They cure well at 125-150°C without the need to add a strong acid catalyst. The acidity of most polymers used in thermoset coatings is sufficient to catalyze their reaction. Their film performance properties are similar to those of the high imino resinsin the previous category. In addition to reacting with the hydroxyl, carboxyl and amide functionality of polymers they, too, can self-condense readily. Their practical equivalent weight is also 180-240. As with all melamine resins, they can be stabilized by adding amine and stabilizing alcohol to the formulation. The major limitation of these products is high formaldehyde release on baking, primarily due to their high free methylol content.Product availability can vary by usage location. Please contact your local Allnex representative regarding availability in specific countries and regions.5Highly Alkylated Melamine ResinsThe highly alkylated melamine resins in thiscategory are similar to the commercial versions of hexamethoxymethylmelamine (HMMM) except for the type of alkylation alcohol. The resins contain combinations of methoxy sites and longer chain length alkoxy sites (ethoxy, n-butoxy or iso-butoxy). They also differ from each other in their degree of alkylation and monomer content. Longer chain length alkoxy sites impart lower viscosity, improved flow and leveling, and intercoat adhesion. All of the resins in the series are efficient crosslinking agents for hydroxyl, carboxyl and amide functional polymers. The practical equivalent weight for most is 140-200. Other advantages are low VOCs; high film flexibility and toughness when used with inherently flexible backbone resins; excellent formulation stability, especially in waterborne systems at a pH of 8-9 and good mar resistance properties. Because of their high extent of alkylation, the resins in this series require the addition of a strong acid catalyst for acceptable cure response when baked at 125-150°C. Typically, 0.2-0.4% p-toluene sulfonic acid based on total binder solids is recommended. The optimum concentration of acid catalyst depends on the basicity of the other components in the formulation and should be determined experimentally. The use of a blocking amine for the acid catalyst and the addition of a stabilizing alcohol to the formulation should enhance formulation stability.High Solids Mixed Ether Melamine Resins6High Imino Melamine ResinsThe high imino melamine resins in this category are similar to those in the high imino methylated melamine series in that they are partially methylated and highly alkylated. They differ from methylated melamine resins in the type of alkylation alcohol, and they contain combinationsof methoxy sites and n-butoxy sites. The butoxy sites impart improved flow and leveling and intercoat adhesion properties. As in the methylated series, their composition contains primarily alkoxy/imino or alkoxy/NH functionality. The advantages are fast cure response at 120-150°C without the need for strong acid catalyst addition; fast cure response in waterborne formulations; high film hardness; and low formaldehyde release on cure. In addition to reacting with hydroxyl, carboxyl and amide functional polymers, the resins also self-condense readily. Therefore, their practical equivalent weight is typically 200-250. They, too, can be stabilized by amine and stabilizing alcohol addition to the formulation.Product availability can vary by usage location. Please contact your local Allnex representative regarding availability in specific countries and regions.7n-Butylated Melamine Resinsn-butylated resins in this category are very polymeric in nature. They differ in extent of methylolation, butylation and polymerization. In general, higher extents of methylolation and butylation result in more hydrophobic resins with lower viscosities, higher stability, slightly higher film flexibility, film gloss and adhesion but slower cure response. Higher extents of polymerization result in faster film property development but also in higher viscosities, and therefore higher VOCs. None of the resins in this category require the addition of a strong acid catalyst for adequate cure at 125-150°C. Usually, the acidity of the other resin components in the formulation results in sufficient catalysis. In addition to reacting with hydroxyl, carboxyl and amide functionalities on polymers, these resinsself-condense readily. Their practical equivalent weight is typically 220-280 on a solids basis, High concentrations of the melamine resins in the formulation result in high film hardness and improved exterior durability but, possibly, lower film flexibility and lower adhesion properties. They, too, can be stabilized by adding amines and stabilizing alcohol to the formulation.Highly n-Butylated Melamine ResinsHighly butylated melamine resins are similar to the commercial versions of hexamethoxymethylmelamine (HMMM), except they are n-butylated. They also are slightly more oligomeric than their methylated counterparts.The butylation or butoxy sites impart improved flow and leveling and intercoat adhesion properties. However,cure response is slower than that of the resins in the methylated category. Yet, highly butylated melamine resins, are efficient crosslinking agents for hydroxyl, carboxyl and amide functional polymers. The practical equivalent weight is 160-220. Other advantages are high film flexibility and toughness when used with inherently flexible backbone resins; excellent formulation stability; and good mar resistance properties. Because of their high extent of alkylation, the resins require the addition of a strong acid catalyst for acceptable cure response when baked at 125-150°C. Typically 0.2-0.4% p-toluene sulfonic acid based on total binder solids is necessary. The optimum concentration of acid catalyst depends on the basicity of the other components in the formulation and should be determined experimentally. The use of a blocking amine for the acid catalyst and the addition of a stabilizing alcohol to the formulation should enhance formulation stability.Butylated Melamine Resins(1) = Foil solids 45 min at 45°C (2) = Pan solids 60 min at 100°C (3) = Pan solids 120 min at 105°C (4) = Pan solids 120 min at 120°C8n-Butylated High Imino Melamine ResinsButylated high imino melamine resins are similar to those in the high imino methylated melamine series; they are partially methylolated and highly alkylated. They differ from the high imino methylated melamine resins in that they are n-butylated. The butoxy sites impart improved flow and leveling and intercoat adhesion properties.As in the methylated series, their composition contains primarily alkoxy/imino or alkoxy/NH functionality. The advantages are fast cure response at 120- 150°C without the need to add a strong acid catalyst; high film hardness; and low formaldehyde release on cure. In addition to reacting with hydroxyl, carboxyl and amide functionality on polymers, the resins self-condense readily. Therefore, their practical equivalent weight is typically 160-220. They can be stabilized by the addition of an amine and a stabilizing alcohol to the formulation. iso-Butylated Melamine ResinsThe iso-butylated melamine resins in this category are similar to polymeric n-butylated resins and differ only in that they are "iso" rather than "n"-butylated. It is generally believed that iso-butylated melamine resins cure faster than n-butylated resins, although differences in extentof methylolation, alkylation and polymerization are believed to be more significant factors with respect to cure response. The comments made previously concerning the n-butylated resins also apply to the iso-butylated resins.Product availability can vary by usage location. Please contact your local Allnex representative regarding availability in specific countries and regions.910Methylated ResinsMethylated urea resins were designed for waterborne and solvent-based formulations for interior and non-UV resistant applications. They differ from each otherprimarily in their extent of methylolation and methylation. As with other amino resins for coatings, higher extents of alkylation result in improved compatibility with most binders, improved stability; and better flow and leveling, but slower cure response. None of these resins require the addition of a strong acid catalyst for acceptable cure response at 125°C. The acidity of the other components in a formulation usually is sufficient for catalysis. For very low temperature cure systems, those for wood finishes, for example, a 2-component formulation is necessary. In such formulations, the acid catalyst is added to the fully-formulated system just prior to use. If the bake temperature is around 70°C, a concentration of 1-2% on total binder solids of a strong or weak acid is recommended. In general, urea resins react with thehydroxyl, carboxyl and amide functional sites on polymers, but they also have a high tendency for self-condensation. Their practical equivalent weight is in the range of 180- 260. The use of a blocking amine for the acid catalyst and the addition of a stabilizing alcohol to the formulation enhances formulation stability. The latter approach is recommended for 2-component formulations.Butylated resinsThe one resin in this category, CYMEL U-80 resin, issimilar in composition to several of the methylated resins, except that its alkylation alcohol is n-butanol. This resin is also recommended for interior and non-UV resistant coatings; it is used primarily in solvent-based systems. It is very hydrophobic and stable but slower curing than its methylated counterparts. A strong acid catalyst is recommended for acceptable cure response at 125°C. Other formulation details are the same as those given for methylated resins.High Solids Urea ResinsHigh Solids Urea ResinsProduct availability can vary by usage location. Please contact your local Allnex representative regarding availability in specific countries and regions.11n-Butylated Urea ResinsThe urea resins in this category are all partially n-butylated and very polymeric in nature. They differ in extent of methylolation, butylation, and polymerization. In general, higher extents of methylolation and butylation result in more hydrophobic resins with lower viscosities, higher stability, and slightly higher film toughness, film gloss and adhesion but slower cure response. Higher extents of polymerization result in faster film property development but also in higher viscosities and, therefore, higher VOCs. None of the resins require the addition of a strong acid catalyst for adequate cure at 125°C. Usually, the acidity of the other resin components in the formulation is sufficient for catalysis. In addition to reacting with hydroxyl, carboxyl and amide functional polymers, the resins also selfcondense readily. Their practical equivalent weight is typically 220-300 on a solids basis. High concentrations of the urea resins in the formulation result inhigh film hardness, but, possibly, lower film toughness and lower adhesion properties. They can be stabilized by amine and stabilizing alcohol addition to the formulation. As with other urea resins,they are recommended for interior, non-UV resistant applications. The most typical applications are interior container coatings andButylated Urea Resins(1) = Pan solids 90 min at 105°C (2) = Pan solids 120 min at 105°C (3) = Pan solids 120 min at 120°C(4) = Pan solids 60 min at 100°C122-component solvent-based wood finishes, In the latter application, the acid catalyst is added to the fully-formulated system just prior to use. A concentration of 1-2% on total binder solids of a strongor weak acid is recommended for systems that require low bake temperatures - typically less than 70°C.iso-Butylated Urea ResinsThe iso-butylated urea resins in this category are similar tothe polymeric n-butylated resins, except that they are iso-butylated rather than n-butylated. It is generally believed that iso-butylated urea resins cure faster than n-butylated resins, although differences in extent of methylolation, alkylation and polymerization are believed to be more significant factors with respect to cure response. iso-butylated urea resins performin much the same way as n-butylated urea resins. That is, high concentrations of these resins in the formulation result in high film hardness, but, possibly, lower film toughness and lower adhesion properties. iso-butylated resins can be stabilized by adding amine and stabilizing alcohol to the formulation. They are recommended for interior and non·UV resistant applications, as well.Product availability can vary by usage location. Please contact your local Allnex representative regarding availability in specific countries and regions.1314Benzoguanamlne ResinsBenzoguanamine resins are similar to melamine-based resins in that they, too, are triazine based, but in this instance, the triazine has a benzene group substitution. Therefore, they are less functional than melamine-based resins, and are not UV resistant. Benzoguanamine resins are noted for their enhanced film flexibility, or toughness, and for their chemical resistance. They are typically used in primers, container coatings, and appliance finishes. Usually, highly alkylated, monomeric benzoguanamine resins result in higher film flexibility than that which can be achieved with other types of resins, but they alsorequire the addition of a strong acid catalyst for adequate cure at temperatures greater than 125°C. Their practical equivalent weights are slightly higher than the practicalweights of their melamine counterparts and range from 160-220. The less alkylated polymeric resins only require the acidity of the other components in the formulation to cure adequately at greater than 125°C, but they have higher VOCs in a solvent-based formulation. Their practical equivalent weights are also slightly higher than those of their melamine counterparts and range from 200-260 on a solids basis. The use of a blocking amine and the addition of a stabilizing alcohol to the formulation should enhance formulation stability.(1) = Pan solids 120 min at 120°CBenzoguanamine and Glycoluril Resins(2) = Foil solids 45 min at 45°C(3) = Pan solids 120 min at 105°CGlycoluril ResinsGlycoluril resins are similar to their melamine resinscounterparts in that they, too, are based on a ring structure,glycoluril. Similar to benzoguanamine resins, they areless functional than melamine resins but are UV resistantand can be used in exterior coatings. Their advantagesare film toughness and flexibility, ability to adhere tometals, and low formaldehyde release on curing. There aretwo categories in the product line, unalkylated methylolglycoluril resins, and those which are highly alkylated.CYMEL 1172 is unalkylated, and was designed for water-based coatings with low temperature cure performanceproperties. The highly alkylated resin, CYMEL 1170, wasdesigned to replace HMMM where there is a desire toimprove film flexibility. The same comments concerninghighly alkylated monomeric melamine resins also apply tothe alkylated glycoluril resins.Product availability can vary by usage location. Please contact your local Allnex representative regarding availability in specific countries and regions.15CYMEL® NF 2000CYMEL NF 2000 crosslinking agent is a unique trifunctional melamine-based crosslinker containing reactive carbamate functionality. It can be used to crosslink hydroxy- and epoxy containing polymers to give highly durable, acid-resistant films that exhibit a favorable balance of hardness and flexibility. An important feature of CYMEL NF 2000is that it does not contain or emit formaldehyde on cure.It can be employed as the sole crosslinker in a coatingor ink formulation, or it may be used at lower levels in combination with other crosslinkers, such as conventional amino resins or isocyanates, in order to obtain a balance of properties. CYMEL NF 2000 is recommended for high quality durable finishes such as automotive topcoats, exterior can varnishes and coil coatings. CYMEL NF 2000 reacts with hydroxy functional backbone resins at >125°C bake schedules to form urethane linkages. Cure can typically be accomplished without use of a catalyst to yield films that exhibit excellent hardness, chemical resistance, exterior durability and environmental etch resistance. Addition of low levels of acid catalyst (e.g. 0.5% dodecyl benzene sulfonic acid on binder solids) has been found to improve humidity resistance properties in some systems. Both catalyzed and uncatalyzed formulations show good 1K package stability at 23°C. CYMEL NF 2000 will react with carboxy functional backbone resins, but require bake temperatures >150°C. CYMEL NF 3041CYMEL NF 3041 is a partially n-butylated crosslinker agent designed for 2 pack ambient and heat cured formulations for industrial wood and plastic applications. These systems have excellent early hardness, resistance properties, appearance and hot/cold cycle flexibility. CYMEL NF 3041 is a very effective crosslinking agent for alkyd, polyester and acrylic polymers containing primary hydroxyl functionality. CYMEL NF 3041 resin requires the addition of an acid catalyst to the formulation in order to obtain effective cure for both ambient and heat cured applications. It is recommended to use 2.0% CYCAT 500 based on weightof total binder solids. However, the acidity of other formulation components may affect the reaction rate and should be evaluated in combination with the catalyst. Catalyzed potlife can be extended by the addition of 10 to 20% primary alcohol on total binder solids. Methanol or ethanol is preferred to ensure early hardness development and sandability. The coatings demonstrate very good flow, gloss, early film hardness, early print resistance and chemical resistance.Formaldehyde Free Resins(1) = Foil solids 45 min at 45°C(2) = Pan solids 120 min at 105°C16Product availability can vary by usage location. Please contact your local Allnex representative regarding availability in specific countries and regions.1718All About ResinsNotice: Trademarks indicated with the ® or ™ are registered, unregistered or pending trademarks of Allnex Belgium SA or its directly orindirectly affiliated Allnex Group companies.Disclaimer: Allnex Group companies (“Allnex”) decline any liability with respect to the use made by anyone of the information containedherein. The information contained herein represents Allnex's best knowledge thereon without constituting any express or implied guaranteeor warranty of any kind (including, but not limited to, regarding the accuracy, the completeness or relevance of the data set out herein).Nothing contained herein shall be construed as conferring any license or right under any patent or other intellectual property rights of Allnexor of any third party. The information relating to the products is given for information purposes only. No guarantee or warranty is providedthat the product and/or information is adapted for any specific use, performance or result and that product and/or information do notinfringe any Allnex and/or third party intellectual property rights. The user should perform its own tests to determine the suitability for aparticular purpose. The final choice of use of a product and/or information as well as the investigation of any possible violation of intellectualproperty rights of Allnex and/or third parties remains the sole responsibility of the user.©2014 Allnex Group. All Rights Reserved.19XLR3001-EN-WW-0314 AFRICAASIAAUSTRALIAEUROPENORTH AMERICASOUTH AMERICAAllnex Global Operational HeadquartersSquare Marie Curie 111070 Anderlecht – BrusselsBELGIUM。
中天经纬代理美国G-Biosciences蛋白试剂盒
3ml 15ml 5x1 ml Col 10x0.2ml Col
3799.6 2560.6 500次/微孔2500次 1168.2 1000次/微孔5000次 1888 500ul 500 500ul 10g 10g 1 Liter 1 Liter 1 Liter 500 377.6 566.4 755 826 578.2
PVDF膜（0.45um）(milipore欲收购的品牌 迈博瑞) PVDF膜（0.22um）(milipore欲收购的品牌 迈博瑞) NC膜（0.45um） (milipore欲收购的品牌 迈博瑞) NC膜（0.22um） (milipore欲收购的品牌 迈博瑞) 灭菌聚醚砜针头滤器, 孔径:0.22μm, 直径:30mm (迈博瑞品牌) 灭菌聚醚砜针头滤器, 孔径:0.45μm, 直径:30mm (迈博瑞品牌) 灭菌聚偏氟乙烯针头滤器, 孔径:0.22μm直径:30mm (迈博瑞品牌) 灭菌聚偏氟乙烯针头滤器, 孔径:0.45μm直径:30mm (迈博瑞品牌) Swift 快速免疫印迹膜染色试剂(“丽春蓝”) 30s超快速染色 NAP-BLOCKER™非动物源的封闭剂 [2X] 封闭效果可达100% Tween® 20 Tween® 20 Tween® 80 Tween® 80 超级ECL化学发光底物(可检测到飞克级，超级灵敏) femtoLUCENT™ PLUS HRP Substrate only for 25 mini blots or 1,500cm2 支原体PCR检测试剂盒 支原体去除试剂 BIOMYC-3 100X (超级管用)复旦/中科院系统推荐 使用 支原体去除试剂 BIOMYC-1 100X (超级管用)交大系统推荐使用 支原体去除试剂 BIOMYC-2 100X (超级管用)交大系统推荐使用 二氧化碳培养箱水盘消毒液 100X (强烈推荐使用，无毒，不腐蚀) 水浴锅消毒液 500X (细胞房必备产品) Pharmacidal 支原体预防喷雾剂 1X (细胞房必备产品) 密理博发光液
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applicationregionitem no.price1p36 deletion syndrome1p36P147-025R€ 237,001p36 deletion syndrome1p36P147-050R€ 474,001p36 deletion syndrome1p36P147-100R€ 948,0022q1122q11P324-025R basic research€ 237,00 € 165,9022q1122q11P324-050R basic research€ 474,00 € 331,8022q1122q11P324-100R basic research€ 948,00 € 663,609q subtelomeric deletion syndrome (9qSTDS) orKleefstra syndrome.EHMT1 P340-025R€ 237,00
9q subtelomeric deletion syndrome (9qSTDS) orKleefstra syndrome.EHMT1 P340-050R€ 474,009q subtelomeric deletion syndrome (9qSTDS) orKleefstra syndrome.EHMT1 P340-100R€ 948,00ALL, iAMP21iAMP21, RUNX1, ERGP327-025R€ 237,00ALL, iAMP21iAMP21, RUNX1, ERGP327-050R€ 474,00ALL, iAMP21iAMP21, RUNX1, ERGP327-100R€ 948,00ALLXp22.33P329-025R€ 237,00ALLXp22.33P329-050R€ 474,00ALLXp22.33P329-100R€ 948,00ALLIKZF1, PAX5, ETV6, RB1, BTG1,EBF1, CDKN2A-CDKN2B,Xp22.33P335-025R€ 237,00
ALLIKZF1, PAX5, ETV6, RB1, BTG1,EBF1, CDKN2A-CDKN2B,Xp22.33P335-050R€ 474,00ALLIKZF1, PAX5, ETV6, RB1, BTG1,EBF1, CDKN2A-CDKN2B,Xp22.33P335-100R€ 948,00ARSACS (autosomal recessive spastic ataxia ofCharlevoix-Saguenay)3q12.12P441-025R€ 237,00ARSACS (autosomal recessive spastic ataxia ofCharlevoix-Saguenay)3q12.12P441-050R€ 474,00ARSACS (autosomal recessive spastic ataxia ofCharlevoix-Saguenay)3q12.12P441-100R€ 948,00Acute lymphoplastic leukemia (ALL), CMLIKZF1 7p12.2P202-025R€ 237,00Acute lymphoplastic leukemia (ALL), CMLIKZF1 7p12.2P202-050R€ 474,00Acute lymphoplastic leukemia (ALL), CMLIKZF1 7p12.2P202-100R€ 948,00Adult Polycystic Kidney Disease PKD1, PKD2P352-025R improved€ 237,00Adult Polycystic Kidney Disease PKD1, PKD2P352-050R improved€ 474,00Adult polycystic kidney disease PKD1P351-025R improved€ 237,00Adult polycystic kidney disease PKD1P351-050R improved€ 474,00Adult polycystic kidney disease PKD1P351-100R improved€ 948,00AgammaglobulinemiaBTK Xq21.3-q22P210-025R€ 237,00
www.MLPA.com - product list - complete probemix list2015-04-25 14:43copyright © 2015 MRC-Hollandpage 1 of 36AgammaglobulinemiaBTK Xq21.3-q22P210-050R€ 474,00Age-related macular degeneration (ARMD) CFH, CFHR3, CFHR1, CFHR2,1q23P236-025R basic research€ 237,00 € 118,50
Age-related macular degeneration (ARMD) CFH, CFHR3, CFHR1, CFHR2,1q23P236-050R basic research€ 474,00 € 237,00Age-related macular degeneration (ARMD) CFH, CFHR3, CFHR1, CFHR2,1q23P236-100R basic research€ 948,00 € 474,00Aicardi-Goutieres Syndrome RNASEH2A, B & C genes, TREX1,SAMHD1P388-100R€ 948,00Alagille Syndrome (AGS)JAG1 20p12.2P184-025R€ 237,00Alagille Syndrome (AGS)JAG1 20p12.2P184-050R€ 474,00Alagille Syndrome (AGS)JAG1 20p12.2P184-100R€ 948,00Albright hereditary osteodystrophy (AHO),Pseudohypoparathyroidism (PHP)GNAS 20q13.32ME031-025R€ 237,00
Albright hereditary osteodystrophy (AHO),Pseudohypoparathyroidism (PHP)GNAS 20q13.32ME031-050R€ 474,00Albright hereditary osteodystrophy (AHO),Pseudohypoparathyroidism (PHP)GNAS 20q13.32ME031-100R€ 948,00Alpha-Thalassemia/Mental RetardationATRX ; Xq13P013-025R€ 237,00Alpha-Thalassemia/Mental RetardationATRX ; Xq13P013-050R€ 474,00Alpha-Thalassemia/Mental RetardationATRX ; Xq13P013-100R€ 948,00Alport Syndrome02q36.3P439-025R€ 237,00Alport Syndrome02q36.3P439-050R€ 474,00Alport Syndrome02q36.3P439-100R€ 948,00Alport syndrome, Hereditary NephritisCOL4A5 Xq22P191-025R€ 237,00Alport syndrome, Hereditary NephritisCOL4A5 Xq22P191-050R€ 474,00Alport syndrome, Hereditary NephritisCOL4A5 Xq22P192-025R€ 237,00Alport syndrome, Hereditary NephritisCOL4A5 Xq22P192-050R€ 474,00Alveolar capillary dysplasia2p24.3 (MYCN) and 16q24.1(FOXF1; FOXC2; FOXL1)P431-025R€ 237,00
Alveolar capillary dysplasia2p24.3 (MYCN) and 16q24.1(FOXF1; FOXC2; FOXL1)P431-050R€ 474,00Alveolar capillary dysplasia2p24.3 (MYCN) and 16q24.1(FOXF1; FOXC2; FOXL1)P431-100R€ 948,00Alzheimer disease, early-onsetAPP 21q21.3P170-025R€ 237,00Alzheimer disease, early-onsetAPP 21q21.3P170-050R€ 474,00Alzheimer disease, early-onsetAPP 21q21.3P170-100R€ 948,00Alzheimer's disease (AD) PSEN1 14q24.2P254-025R€ 237,00Alzheimer's disease (AD) PSEN1 14q24.2P254-050R€ 474,00Alzheimer's disease (AD) PSEN1 14q24.2P254-100R€ 948,00Alzheimer's disease, Pick's disease, frontotemporaldementia, cortico-basal degeneration, progressivesupranuclear palsyMAPT 17q21P275-025R€ 237,00
www.MLPA.com - product list - complete probemix list2015-04-25 14:43copyright © 2015 MRC-Hollandpage 2 of 36