Tree-Penguin Interference and Tests for $cosgamma 0$ in Rare $Bto PP$, $PV$ and $VV$ Decay
生活大爆炸第三季S3E10 中英文对照剧本

他说的很多话本是故意为了幽默一下的 A lot of what he says is intended as humor. 是啊 但我一点也不觉得有趣 Yeah, well, I don't think it's very funny. 我也是 但是我一笑他就灿烂了 Me neither, but he just lights up when I laugh. 霍华德 不能让她跑了 Howard, never let her go. 莱纳德 霍华德说你正在进行 So, Leonard, Howard says you're working on 一些量子力学的基本测试 fundamental tests of quantum mechanics. 没错 I am. 你对物理感兴趣吗 Are you interested in physics? 我觉得很很吸引人 Oh, I find it fascinating. 如果我没有选择微生物学的话 If I hadn't gone into microbiology, 我也许就会进军物理了 I probably would have gone into physics. 或者冰舞 Or ice dancing. 事实上 我对于阿哈伦诺夫-博姆的 Actually, my tests of the Aharonov-Bohm 量子干涉效应实验已经到达了一个很有趣的阶段 quantum interference effect have reached an interesting point. 现在 我们正在测试基于电势的 Right now, we're testing the phase shift 相位偏移 due to an electric potential. 真是太棒了 That's amazing. 那是 莱纳德的工作几乎就跟 Yes. Leonard's work is nearly as amazing 三年级小学生用湿毛巾种青豆一样棒 as third graders growing lima beans in wet paper towels. 虽然我很欣赏你的 "喔 又损人了" While I appreciate the "Oh, snap," 但你那温湿的口气飘进我的耳中令我很不舒服 I'm uncomfortable having your moist breath in my ear. 你会用 Are you going to try
FluorCam叶绿素荧光成像文献 2009 Feeding enhances photosynthetic efficiency

Feeding enhances photosynthetic efficiency in the carnivorous pitcher plantNepenthes talangensisAndrej Pavlovicˇ*,Lucia Singerova ´,Viktor Demko and Ja ´n Huda ´k Department of Plant Physiology,Faculty of Natural Sciences,Comenius University,Mlynska´dolina B-2,SK-84215,Bratislava,Slovak RepublicReceived:10March 2009Returned for revision:30March 2009Accepted:2April 2009Published electronically:19May 2009†Background and Aims Cost–benefit models predict that carnivory can increase the rate of photosynthesis (A N )by leaves of carnivorous plants as a result of increased nitrogen absorption from prey.However,the cost of car-nivory includes decreased A N and increased respiration rates (R D )of trapping organs.The principal aim of the present study was to assess the costs and benefits of carnivory in the pitcher plant Nepenthes talangensis ,leaves of which are composed of a lamina and a pitcher trap,in response to feeding with beetle larvae.†Methods Pitchers of Nepenthes grown at 200m mol m 22s 21photosynthetically active radiation (PAR)were fed with insect larvae for 2months,and the effects on the photosynthetic processes were then assessed by simul-taneous measurements of gas exchange and chlorophyll fluorescence of laminae and pitchers,which were corre-lated with nitrogen,carbon and total chlorophyll concentrations.†Key Results A N and maximum (F v /F m )and effective quantum yield of photosystem II (F PSII )were greater in the fed than unfed laminae but not in the fed compared with unfed pitchers.Respiration rate was not significantly affected in fed compared with unfed plants.The unfed plants had greater non-photochemical quenching (NPQ)of chlorophyll fluorescence.Higher NPQ in unfed lamina did not compensate for their lower F PSII ,result-ing in lower photochemical quenching (QP)and thus higher excitation pressure on PSII.Biomass and nitrogen and chlorophyll concentration also increased as a result of feeding.The cost of carnivory was shown by lower A N and F PSII in pitchers than in laminae,but R D depended on whether it was expressed on a dry weight or a surface area basis.Correlation between nitrogen and A N in the pitchers was not found.Cost–benefit analysis showed a large beneficial effect on photosynthesis from feeding as light intensity increased from 200to 1000m mol m 22s 21PAR after which it did not increase further.All fed plants began to flower.†Conclusion Feeding pitchers with insect larvae increases A N of leaf laminae,due to higher nutrient acquisition,with strong correlation with nitrogen concentration,but A N of pitchers does not increase,despite increased nitro-gen concentration in their tissue.Increased A N improves growth and reproduction and is likely to increase the competitive advantage of carnivorous over non-carnivorous plants in nutrient-poor habitats.Key words:carnivorous plants,chlorophyll fluorescence,Nepenthes talangensis ,nitrogen,pitcher plant,photosynthetic rate,photosystem II,respiration rate.INTRODUCTIONCarnivorous pitcher plants of the genus Nepenthes are largely found in south-east Asia,principally Borneo,Sumatra,Java and peninsular Malaysia,with scattered populations in India,Sri Lanka,Australia,New Caledonia,Madagascar and the Seychelles.Nepenthes leaves are differentiated into a photo-synthetically active lamina and a pitcher trap,which has evolved to attract,trap and digest prey.The pitchers usually consist of different structural and functional zones:lid,peri-stome,and upper waxy and lower glandular zones within the pitcher (Clarke,1997,2001).Carnivorous plants grow terrestrially in sunny,nutrient-poor and permanently moist habitats.Cost–benefit models of car-nivory predict that in a well-lit environment the nutritional benefits gained from captured prey exceed the costs of modify-ing leaves into photosynthetically inefficient traps (Givnish et al.,1984).Generally,costs of carnivory include energetic demands for growth of traps and their function:either increased rate of dark respiration (R D )as a result of extraenergy requirements for attracting,capturing and digesting the prey,or decreased photosynthetic rate (A N )as a result of leaf adaptation for carnivory,or both.Three potential benefits resulting from increased mineral absorption from prey have been proposed.First,carnivory may increase a plant’s rate of photosynthesis (A N )through improved nutrient supply,particu-larly nitrogen status,although other nutrients (principally phosphate and potassium)may be important.Second,carniv-ory may results in an increased seed production through improved mineral acquisition;and third,carnivory may replace autotrophy partly by heterotrophy (Givnish et al.,1984).Givnish et al.(1984)considered the second benefit as a part of the first,as increased A N should lead to increased seed production.Several authors have dismissed the third benefit,as experimental findings suggest that carnivorous plants do not obtain substantial amounts of carbon from prey and carnivory could not replace autotrophy at low light inten-sity (Chandler and Anderson,1976).However,Rischer et al.(2002)found that Nepenthes incorporated carbon from carniv-ory into organic substances,which raises a question about the importance of facultative heterotrophy.The growth of some*For correspondence.E-mail pavlovic@fns.uniba.sk#The Author 2009.Published by Oxford University Press on behalf of the Annals of Botany Company.All rights reserved.For Permissions,please email:journals.permissions@Annals of Botany 104:307–314,2009doi:10.1093/aob/mcp121,available online atby guest on March 15, 2012/Downloaded fromspecies of carnivorous plants is partly dependent on organiccarbon uptake from prey,as revealed by increased growth without increasing A N (Adamec,1997,2008).With regard to the costs of carnivory,it has been observed that photosynthetic rates of traps are lower than those ofleaves (Knight,1992;Adamec,2006;Pavlovicˇet al.,2007).With regard to the benefits,around 30studies have tested whether the growth of carnivorous plants is enhanced by carnivory.Ellison (2006)concluded that there is a significant positive effect (P ¼0.02)of adding prey on plant growth among different carnivorous genera,supporting the hypothesis that there is a benefit to carnivory.However,he pointed out that this is only indirect evidence,because the cost–benefit model expresses benefits in terms of photosynthetic rates,not in terms of growth.Only three studies have examined the effect of prey capture on A N of terrestrial carnivorous plants directly,and these gave very different results.Convincing evidence that prey availability increased absolute A N in Sarracenia has been provided by Farnsworth andEllison (2008).However,Me´ndez and Karlsson (1999)and Wakefield et al.(2005)did not find any changes in A N in Pinguicula vulgaris and Sarracenia purpurea in response to the capture of prey.Either of two possible results can be expected from feeding prey to carnivorous plants.First,fed plants will have greater biomass but nitrogen (N)concentration (mg N g 21d.wt)will not be affected,despite total N (mg)per plant increasing.This was observed by Moran and Moran (1998)in Nepenthes rafflesiana .In extreme cases,N concentrations per unit dry matter might even decrease due to dilution by increased growth (Karlsson and Carlsson,1984;Adamec,2008).Because N concentration is positively correlated with A N in carnivorous plants (Ellison and Farnsworth,2005;Pavlovicˇet al.,2007),it can be expected that A N will not be enhanced,as found by Me´ndez and Karlsson (1999)in Pinguicula vulgaris .In this case,the cost–benefit model must be considered in terms of growth rate partly due to direct uptake of organic compounds from prey.The second possibility is that fed plants will have higher leaf N concentrations and,therefore,higher A N as predicted by Givnish et al.(1984).This was found by Farnsworth and Ellison (2008)in Sarracenia .The principal aim of the present study was to assess the costs and benefits of carnivory in Nepenthes ,which provides a good experimental model for the study of the cost–benefit model of carnivory because the leaves are divided into photo-synthetically active laminae and a pitcher trap.We assumed that increased photosynthesis is the most important benefit of carnivory,and tested the hypothesis that prey availability would result in increased A N ,photosystem II (PSII)efficiency,biomass,and nutrient and chlorophyll concentrations and that the cost of carnivory would include increased R D and decreased A N and PSII efficiency in the pitcher sensu stricto according to the Givnish hypothesis (Givnish et al.,1984).Tests were made on the pitcher plant Nepenthes talangensis ,a rare,endemic species from Sumatra.This is the first detailed study of photosynthesis in a carnivorous plant,with and without experimental addition of prey,using simultaneous measurements of gas exchange and chlorophyll fluorescence by the saturation pulse method.MATERIALS AND METHODSPlant material and culture conditionsThe pitcher plant Nepenthes talangensis Nerz and Wistuba (1994)grows in mossy forest and stunted upper mountain forest near the summit of Gunung Talang (1800–2500m alt.)in Sumatra (Nerz and Wistuba,1994;Clarke,2001).Its pitchers are light green to yellow in colour with red spots,lack a waxy zone (glandular region covers entire inner surface)and the pitcher fluid is extremely viscous (Clarke,2001).Five-year-old plants,propagated from seeds,were 20–30cm tall,with three or four pitchers up to 5cm long.During the experiments,ten plants were grown under con-trolled conditions in a growth chamber with a photoperiod of 12h dark/12h light [200m mol m 22s 21photosynthetically active radiation (PAR),day/night temperatures of 25/178C and high humidity (80–100%)].They were grown in a Sphagnum /perlite/bark/moss mixture substrate.To prevent entry of prey into pitchers they were plugged,without dama-ging them,with wads of cotton wool moistened in distilled water.Any newly opened pitchers during experiments were treated in the same way.The wads were removed from five plants after 6months.The remaining five plants served as unfed controls.The fed plants were supplied with one live meal worm (Tenebrio molitor )for each pitcher each week for 8weeks (total 2.46+0.05g f.wt worms per plant over 8weeks,N concentration in worms ¼8.7%,78mg N per plant over 8weeks).Fed plants were also able to catch natural prey,mostly sciarid flies,but the contribution of these to the nutrition of fed plants was negligible (,2%of a worm’s weight).Simultaneous measurement of CO 2assimilation and chlorophyll fluorescenceTo assess whether feeding enhanced photosynthetic effi-ciency,we analysed five youngest fully developed laminae (one per plant)with un-formed pitchers that had developed during the 8-week feeding period (‘young lamina without pitcher’),and five older laminae carrying the pitcher (separ-ated into ‘older lamina with pitcher’and ‘pitcher’),which had developed before the feeding experiment had started.Rates of photosynthesis (A N )and chlorophyll fluorescence were measured simultaneously with a CIRAS-2(PP-Systems,Hitchin,UK)and a fluorcam FC 1000-LC (Photon Systems Instruments,Brno,Czech Republic)attached to an infrared gas analyser.Prior to measurements,the plants were dark-adapted overnight to achieve fully relaxed non-photochemical quenching (NPQ).Thereafter,the middle part of the lamina and the lid in the case of the pitcher (2.5cm 2)were enclosed in the leaf cuvette (PLC6,PP-Systems).Once stabilization (15min)was achieved the respiration rate (R D )was recorded.Then the chlorophyll fluorescence was measured.Minimal fluorescence (F 0)and then maximal fluorescence (F m )were measured using a saturation pulse (4000m mol m 22s 21PAR,800-ms duration):maximal quantum yield of PSII (F v /F m )was calculated as F m –F 0/F m .An induction curve of 15min duration was then obtained by switching on an actinic light of 200m mol m 22s 21PAR.For analysis of the quenching mechanism,ten saturation pulses were triggered.Pavlovicˇet al.—Photosynthetic response to feeding in Nepenthes 308 by guest on March 15, 2012/Downloaded fromSimultaneously,stable A N was recorded at a CO 2concentrationof 360m mol mol 21,leaf temperature 23+18C,relative air humidity 65–70%and water vapour deficit 700–1000Pa.Effective quantum yield of photosystem II (F PSII ),photoche-mical quenching (QP)and NPQ were calculated (Maxwell and Johnson,2000).The saturation irradiance(1200m mol m 22s 21PAR)was applied for 15min to allow adaptation,and light response curves were determined.The light intensity was decreased stepwise with irradiation periods of 3min and subsequent saturation pulses were applied until 40m mol m 22s 21PAR was reached.The appar-ent quantum yield of CO 2fixation (F CO2)was determined as the slope of the light response curve between 40and 150m mol m 22s 21PAR (Farquhar et al.,1980).Light response curves of A N ,F PSII and NPQ were recorded simul-taneously.All measurements were taken between 0900and 1200h.Chlorophyll,nitrogen and carbon determinationThe leaves from five fed and unfed plants were removed.Parts of the leaves were dried at 708C for 5days to determine percentage dry weight.Chlorophyll concentrations were deter-mined on the same types of leaves on which A N had been measured.Samples of leaves from young laminae without pitchers,older laminae carrying pitchers and pitchers them-selves were ground in a mortar and pestle with small amount of sand and extracted with 80%(v/v)chilled acetone with MgCO 3to avoid acidification and phaeophytinization of pig-ments.The samples were centrifuged at 8000g for 5min at 48C.Chlorophyll a þb (chl a þb )in supernatant were deter-mined spectrophotometrically (Jenway 6400,London,UK):chl a at 663.2nm,chl b at 646.8nm.Chlorophyll concen-tration (mg L 21)was calculated according to Lichtenthaler (1987)and re-expressed as mg chl a þb g 21d.wt.Leaf tissues from photosynthetic measurements were dried at 708C for 5days and N and C were determined using an EA 1108CHN analyser (Fisons Instruments,Milan,Italy).Nepenthes pitchers were washed using distilled water before drying and analysing to avoid contamination with nitrogen from prey.After N determination,photosynthetic nitrogen use efficiency (PNUE)was calculated for each type of leaf as:PNUE (m mol CO 2mol N 21s 21)¼A Nmax (m mol CO 2g 21d.wt s 21)/N (mol N g 21d.wt).Statistical analysisPrior to statistical tests,data were analysed for normality and homogeneity of variance.When non-homogeneity was present,a t -test was employed with the appropriate corrected degrees of freedom.To evaluate the significance of the data between fed and unfed plants [leaf dry weight,R D ,A Nmax ,sto-matal conductance (g s ),F v /F m ,F PSII ,F CO2,QP,NPQ,C,N,PNUE,chl a þb ,chl a /b ]a t -test was used.Paired data (com-parison between the lamina and the pitcher within the same old leaf carrying the pitcher)were statistically evaluated by a two-tailed paired t -test.The results are expressed as the mean of five replicated measurements.ANCOV A (StatistiXL ver.1.7for Microsoft Excel)was used to test the homogeneity ofslopes of the relationships between A N and N content for lamina and pitcher.RESULTSFeeding the pitchers of Nepenthes with beetle larvae increased the dark and light reactions of photosynthesis.In the laminae,A N increased almost linearly with increasing irradiance at irra-diances less than about 160m mol photon m 22s 21PAR and reached saturation under an irradiance of about 1000m mol photon m 22s 21PAR (Fig.1A,B).The A N of the young fed lamina without pitcher was significantly higher than the unfed control (Table 1).The A Nmax of laminae from unfed plants was about 50%that of fed lamina at saturating irradi-ance (Fig.1A).Consistent with this,effective quantum yield of PSII (F PSII )and apparent quantum yield of CO 2fixation (F CO2)were also higher in young laminae from plants that had been fed (Table 1).F PSII decreased with increasing irradi-ance (Fig.1D).Fed laminae had F v /F m values of about 0.800,significantly greater than those of laminae from unfed plants.The primary electron acceptor from PSII (plastoquinone A,Q A )was more reduced in unfed plants,based on the higher value of QP in fed plants (Table 1).NPQ increased with increasing irradiance:the higher NPQ in laminae from unfed plants suggests greater heat dissipation via the xanthophyll cycle (Fig.1G).Chlorophyll concentrations and chlorophyll a/b ratios were greater in fed plants than in unfed plants (Tables 1and 2),indicating an increased proportion of light-harvesting complexes (LHC II)to reaction centres (RC II)in PSII in unfed plants.This is consistent with higher values of F 0in unfed plants (data not shown).Lamina dry weight,nitro-gen concentration and PNUE were significantly higher in fed plants.Respiration rate was not significantly different,but there was a trend towards slightly greater R D in fed plants in all tissues studied (Table 1).Differences in measured photosynthetic characteristics between fed and unfed plants in the older laminae carrying the pitcher were similar to those of young laminae except their dry weight (Table 2,Fig.1B,E,H).This is not surprising given that the older laminae were fully developed before the feeding experiment started,whereas the young laminae were developing during the feeding experiment.There were no sig-nificant differences in PNUE between the older laminae of fed and unfed plants.In the pitchers,A N was very low and increased linearly with increasing irradiance at irradiances less than about 50m mol photon m 22s 21PAR and reached saturation only at 100m mol photon m 22s 21(Fig.1C).In contrast to laminae,there were no statistical differences between pitchers of fed and unfed plants in A Nmax ,F v /F m ,F PSII ,F CO2,QP,g s or chlor-ophyll concentration (Table 2).However,unfed pitchers had higher NPQ than fed pitchers and lower nitrogen concen-trations,similar to the pattern in young and old laminae.Almost all photosynthetic parameters were significantly lower in pitchers than in laminae (Table 2).The primary acceptor of PSII,Q A ,was maintained at more than 70%oxi-dized in the lamina,but in the pitcher it was only 25–35%oxidized.NPQ was similar in pitchers and laminae,but was strongly dependent on whether the plants were fed or unfed.However,at higher irradiance,laminae had higher NPQPavlovicˇet al.—Photosynthetic response to feeding in Nepenthes 309by guest on March 15, 2012/Downloaded from(Fig.1H).The NPQ was saturated at 300m mol photon m 22s 21PAR in the pitcher (Fig.1I).Stomatal conductance,PNUE,and nitrogen,carbon and chlorophyll concentrations were also significantly lower in the pitcher.The R D per unit surface area was higher in the lamina.By contrast,R D per unit mass was higher in the pitcher (Table 2).Figure 2summarizes the relationship between nitrogen con-centration and A N in lamina and trap separately.It is obvious that there is a strong correlation between nitrogen concen-tration and A N in laminae (P ,0.01)but not in pitchers (P ¼0.06).Furthermore,the relationships do not have similar slopes (P ¼0.013).One year after the feeding experiment all fed plants,but no unfed plants,had begun to flower.DISCUSSIONThe effects of feeding pitchers with beetle larvae on the photo-synthetic activity of the pitcher plant Nepenthes talangensis was investigated,using simultaneous measurement of gas exchange and chlorophyll fluorescence,and relating them to nitrogen and chlorophyll content of the laminae and pitchers.Nepenthes is a good experimental genus for studying the21034500·10·20·30·40·50·60·7–2–101243567PAR (µmol m –2 s –1)N P QφP S I IA N (µm o l C O 2 m –2 s –1)PAR (µmol m –2 s –1)PAR (µmol m –2 s –1)20004006008001000120002004006008001000120020040060080010001200F IG .1.Rate of net photosynthesis (A N ;A–C),effective quantum yield of PSII (F PSII ;D–F)and non-photochemical quenching (NPQ;G–I)in response to irradiance in young lamina without pitcher (A,D,G),older lamina (B,E,H)and pitcher (C,F,I).Fed and unfed plants as indicated,values are means +s.e.PAR,photosynthetically active radiation.T ABLE 1.Leaf biomass,chlorophyll fluorescence,gas exchange,chlorophyll,nitrogen and carbon concentration,and photosynthetic nitrogen use efficiency in young lamina withoutpitcherParameterUnfed Fed Leaf dry weight (mg)104.5+13.7162.0+12.2*R D (m mol CO 2m 22s 21)1.30+0.161.58+0.15ns R D (nmol CO 2g 21d.wt s 21)8.9+2.211.5+2.2ns A Nmax (m mol CO 2m 22s 21)2.8+0.26.0+0.4**A Nmax (nmol CO 2g 21d.wt s 21)16.9+0.6046.1+7.6**g s (mmol m 22s 21)62.1+4.5152.0+8.2**F v /F m 0.758+0.0110.800+0.003*F PSII0.41+0.020.52+0.01**F CO2(mol CO 2mol quanta 21)0.014+0.0010.023+0.002**QP 0.71+0.010.77+0.01**NPQ1.28+0.130.84+0.06*C (mg g 21d.wt)459.8+2.4468.4+3.1*N (mg g 21d.wt)10.6+1.018.6+2.2*PNUE (m mol CO 2mol N 21s 21)23.7+1.632.5+2.4*Chl a þb (mg g 21d.wt)1.21+0.112.72+0.22**Chl a /b1.91+0.32.15+0.03**See Appendix for definitions.Values shown are means +s.e.,n ¼5.Significantly different values (t -test)are indicated:*P ,0.05,**P ,0.01;ns,non-significant differences.Pavlovicˇet al.—Photosynthetic response to feeding in Nepenthes 310by guest on March 15, 2012/Downloaded fromcost–benefit model of carnivory with leaves composed of photosynthetically active laminae and a pitcher trap.The laminae of fed N.talangensis had a greater N concentration as a result of nitrogen absorption from prey (Tables 1and 2).In their natural environment,Nepenthes species are N-limited,and have evolved the pitcher to assist in their uptake of N (Osunkoya et al.,2007).The average N acquired from insects is high:61.5,53.8and 68.1%of the total for N.mirabilis ,N.rafflesiana and N.albomarginata ,respectively (Schulze et al.,1997;Moran et al.,2001).Chlorophyll concen-tration,A N and maximum and effective quantum yield of PSII were higher in fed plants.Two consequences of this are (1)an increase in biomass of new formed laminae and (2)flowering of plants after feeding (Table 1).Because about 50–80%of foliar N is incorporated in photosynthetic proteins (Evans,1989),we suggest that the lower A N of unfed plants is due to lower N and Rubisco concentrations and thus lower capacity for CO 2fixation.The smaller A N was accompanied by a smaller stomatal conductance (g s )in unfed compared with fed plants but intercellular CO 2concentration (C i )was statisti-cally unchanged (data not shown).This indicates that reduced A N was due to reduced carboxylation efficiency rather than to stomatal limitation.Lower F PSII is a secondary consequence of impaired CO 2assimilation.When carbon fixation is inhib-ited,F PSII is often down-regulated to match the reduced requirement for electrons and to minimize the production of reactive oxygen species (Golding and Johnson,2003).Maximum quantum yield of PSII of dark-adapted leaves,which is proportional to the quantum yield of O 2evolution,was slightly lower in unfed plants,reflecting that potentional quantum yields for photochemistry in PSII were also nega-tively affected in prey-deprived plants (Tables 1and 2).When nutrient stress restricts carboxylation,even moderate light may become excessive and may result in destructive photo-oxidative reactions.In the first line of defence against photo-oxidation,xanthophylls transform excessive excitation energy to heat,measured as NPQ of chlorophyll fluorescence (Krause and Jahns,2004).In laminae,NPQ values were higher in unfed plants as a consequence of less light energy being used in photochemistry and through greater heat dissipa-tion by the xanthophyll cycle.This suggests that increased thermal dissipation by the xanthophyll cycle slightly compen-sates for the lower F PSII in unfed lamina,but probably not sufficiently.The decline of F PSII in unfed laminae was not offset by thermal dissipation,leading to a lower QP and higher excitation pressure on PSII and thus higher susceptibility to photoinhibition in unfed plants.The unfed N.talangensis plants exhibited similar symptoms to plants under nitrogen stress.Huang et al.(2004)found lower A N ,g s ,F v /F m ,F PSII ,QP,chl and chl a /b in nitrogen-deprived rice.012345678N concentration (%)A N (µm o l C O 2 m –2 s –1)F IG photosynthetic rate (A N )in relation to leaf nitrogen concentration in lamina and pitcher,as indicated.The lines have different slopes (P ¼0.013);no relationship was found between A N and N in the pitcher (P ¼0.06)but a significant relationship was found in the lamina (P ,0.01).T ABLE 2.Leaf biomass,chlorophyll fluorescence,gas exchange,chlorophyll,nitrogen and carbon concentration,and photosynthetic nitrogen use efficiency in old lamina carrying the pitcherLaminaPitcherParameterUnfed Fed Unfed FedLeaf dry weight (mg)104.7+15.8120.5+19.6ns 141.3+5.2††199.5+47.8ns ††R D (m mol CO 2m 22s 21)0.97+0.021.15+0.06ns 0.43+0.02††0.60+0.1ns ††R D (nmol CO 2g 21d.wt s 21)5.8+0.57.0+0.21ns 6.5+0.4††9.6+1.7ns ††A Nmax (m mol CO 2m 22s 21)3.1+0.36.0+0.5**0.10+0.04††0.15+0.03ns ††A Nmax (nmol CO 2g 21d.wt s 21)19.4+2.037.8+5.4*2.0+0.9††3.5+1.4ns ††g s (mmol m 22s 21)57.0+6.492.7+4.6**37.7+4.4††31.3+1.6ns ††F v /F m 0.770+0.0100.823+0.011*0.715+0.012†0.740+0.008ns ††F PSII0.41+0.020.59+0.02**0.18+0.01††0.16+0.01ns ††F CO2(mol CO 2mol quanta 21)0.011+0.0020.021+0.001**0.001+0.000††0.001+0.000ns ††QP 0.73+0.020.82+0.01**0.34+0.04††0.25+0.03ns ††NPQ1.64+0.140.79+0.03**1.66+0.14ns 0.68+0.14**ns C (mg g 21d.wt)464.2+1.9477.5+5.8*457.1+0.9††462.2+1.2*††N (mg g 21d.wt)11.3+0.420.0+2.8*7.8+0.2††17.0+1.5**†PNUE (m mol CO 2mol N 21s 21)20.7+1.524.8+4.1ns 4.4+1.2††2.7+0.8ns ††Chl a þb (mg g 21d.wt)1.30+0.012.51+0.34**0.93+0.09††1.02+0.05ns ††Chl a /b1.94+0.022.15+0.06**††0.96+0.041.45+0.07**††See Appendix for definitions.Values shown are means +s.e.,n ¼parisons were made (t -test)between fed and unfed lamina or pitcher at *P ,0.05,**P ,0.01,and between fed pitcher and fed lamina or unfed pitcher and unfed lamina respectively at †P ,0.05,††P ,0.01(paired t-test);ns,non-significant differences.Pavlovic ˇet al.—Photosynthetic response to feeding in Nepenthes 311by guest on March 15, 2012/Downloaded fromAll photosynthetic parameters were significantly lower in pitchers than in laminae (Table 2).Nepenthes pitchers have digestive functions and the absence of a positive correlation between N and A N (Table 2,Figs 1and 2)suggests thatfactors other than N limit A N .Pavlovicˇet al.(2007)suggest high diffusional resistance for CO 2uptake in the Nepenthes pitcher due to very low stomatal density and compact meso-phyll.Low PNUE in the pitchers found in this and in our pre-vious study (Pavlovicˇet al.,2007)indicates either high resistance for CO 2uptake or increased allocation of N to struc-tural materials rather than to photosynthetic machinery (Osunkoya et al.,2007,2008).Lower QP (Table 2)and satur-ation of NPQ at relatively low irradiance in the pitchers (Fig 1I)result in increase excitation pressure on PSII and higher susceptibility to photoinhibition.This may explain the reduced longevity of pitchers relative to laminae,which is well documented (Osunkoya et al.,2008).All the above demonstrate a strong adaptation of pitchers to the carnivorous,but not to the assimilation,function.The only study to date that has quantified the effects of nutrient stress in Nepenthes is that of Moran and Moran (1998),who examined foliar reflectance in nutrient-starved N.rafflesiana .They observed no significant differences in root or leaf N concentration,which is inconsistent with the present results.However,N content (concentration Âbiomass)was lower in their prey-deprived plants.They suggested that increased growth upon feeding is a primary adaptation because under conditions of resource limitation,plants are able to maintain critical foliar nutrient concentrations by a reduction in growth rate.Increased A N after feeding was found by Farnsworth and Ellison (2008)in the genus Sarracenia.The well-fed plants had slightly higher foliar N concentration,chlorophyll content and F v /F m .However,these differences were only found in young unfed Sarracenia leaves that were produced subsequent to feeding.Wakefield et al.(2005)found no changes of A N measured on fed leaves of Sarracenia purpurea .This agrees with the findings of Butler and Ellison (2007),who demonstrated that nutrients captured by older pitchers are rapidly translocated to newly formed leaves.In contrast,we found enhanced A N in older lamina carrying the fed pitcher,although the pitcher itself did not increase in photosynthetic efficiency (Fig.2C,F).The results of Schulze et al.(1997)show that not only young developing leaves carrying closed pitchers obtain a high portion of N from captured prey,but also that older fully developed leaves carrying open pitchers also obtain more than 50%of their nitrogen from prey in Nepenthes .Ellison and Gotelli (2002)showed an increase in A N following addition of inorganic nitrogen to Sarracenia purpurea ,but this response resulted from plants producing non-carnivorous phyllodes,which are more efficient in photosynthesis than the carnivorous pitcher.S.purpurea produces trapless phyllodes only during drought,under shade or with increased nutrient availability.In contrast to Nepenthes ,Sarracenia does not have leaves that are differentiated into a photosynthetically active lamina and a pitcher trap,but usually have only a rosette of pitchers that must function in both photosynthesis and prey capture to achieve positive carbon gain,and their photosynthetic efficiency is closer to Nepenthes lamina than to the Nepenthes pitcher(Pavlovicˇet al.,2007).The rate of photosynthesis was not increased as a result ofprey capture in the carnivorous butterwort Pinguicula vulgaris (Me´ndez and Karlsson,1999).However,supplementary feeding in situ increased both rosette size and reproduction,through an increase in flowering frequency and seed pro-duction (Thore´n and Karlsson,1998).Me ´ndez and Karlsson (1999)also concluded that the benefits from capturing prey are larger in reproductive terms than in terms of photosyn-thesis.We also found accelerated flowering in fed Nepenthes plants,but propose that this is an indirect effect of increased A N rather than a direct effect of feeding.Adamec (2008)observed two different responses to feeding in the aquatic carnivorous plants Utricularia australis and Aldrovanda vesi-culosa .Both species,when fed,produced longer shoots and had smaller N concentrations in comparison with control plants.Photosynthetic rate was higher in fed Aldrovanda but lower in fed Utricularia .It was suggested that tissue N in fed plants was diluted by growth processes much more than in unfed controls,so the main physiological effect of catching prey was not based on enhancement of A N ,as was suggested by Givnish et al.(1984),but on providing N and P (and prob-ably C)for essential growth processes.The present study results contrasted with this,as there was a positive correlation between N and A N in lamina (Fig.2),in agreement with the study of Ellison and Farnsworth (2005),but not of Wakefield et al.(2005).The contradictory results concerning feeding experiments discussed above might lie in genotypic differ-ences among plant species.The Givnish model considers not only the ability of carniv-ory to enhance A N ,but also the costs associated with carniv-ory.The costs include a reduced A N and higher R D .The first was confirmed in this and in our previous study of N.alataand N.mirabilis (Pavlovicˇet al.,2007),but the second is prob-ably species-specific.Different results were obtained here depending on the units of measurements.Area-based R D was higher in the lamina,and mass-based R D was higher in the pitcher.This discrepancy is due to different leaf mass area (LMA was higher in lamina)of these two distinct organs.Differences in leaf thickness between lamina and pitcher are well documented in six Nepenthes species (Osunkoya et al.,2007).It appears that the result is influenced more by leaf structure than by specialization for carnivorous or photosyn-thetic function.Reduced A N and higher R D was found in Utricularia bladder.Photosynthetic rate in leaves of six aquatic Utricularia species exceed that in bladders seven-to ten-fold and R D of bladders was 75–200%greater than in leaves (Adamec,2006).The high R D of bladders in Utricularia is consistent with specific amino acid changes (Leu113,Ser114replaced by Cys113,Cys114)in Utricularia cytochrome c-oxidase,the rate-limiting enzyme in the respirat-ory cycle,which accelerates the rate of respiration.These amino acid changes were not confirmed for Nepenthes (Jobson et al.,2004).According to Givnish,there is a trade-off between photosyn-thetic costs and benefits that could lead to the evolution of car-nivory.Enhancement of A N resulting from the addition of nutrients as a result of carnivory should be more rapid in high-light than in shady environments (Givnish et al.,1984;Ellison and Gotelli,2001).However,convincing evidence for this is lacking.From the present data we can calculate the benefit ofPavlovicˇet al.—Photosynthetic response to feeding in Nepenthes 312 by guest on March 15, 2012/Downloaded from。
托福TPO听力高频词(持续添加版)

新托福听力场景汇总之LECTURE篇1. 生物学antibody抗体toxin毒素immunity免疫immunology免疫学vaccine疫苗fungus真菌bacteria细菌fermentation发酵inflection传染/ 感染microorganism / microbe微生物virus 病毒disfection消毒sterilization灭菌biology生物学marine biology海洋生物学entomology 昆虫学ornithology鸟类学microbiology微生物学genetics遗传学speciology物种学parasitology寄生虫学paleontology古生物学paleontologist古生物学家dinosaur恐龙die out / extinction灭绝mammal哺乳动物carnivore食肉动物rodent啮齿类动物underwater 水下的marine 海洋的scuba 水下呼吸器diving潜水/ 跳水one-celled organism单细胞有机体tissue(动植物细胞的)组织protective camouflage保护色predator捕猎者oceanic snail蜗牛animal adaptation动物适应性survival of the fittest适者生存origin of species物种起源wild environment野生环境insecticide杀虫剂prenatal care 产后护理habitat栖息地tentacle触须prey捕食navigate导航tiny receptor接收器nerve / specimen物种amphibian两栖类动物decline in the number数量减少gene基因genetic基因的,遗传的endangered species濒危动物survival活着的transition转变/过渡microbe微生物yeast 酵母(菌)bacteria 细菌single-cell单细胞reptile爬行类动物hatch孵化incubation孵化nest巢offspring子孙chew up咀嚼unfertilized eggs未受精卵nutrient营养品nourishment营养品/ 食物feed喂养cannibalism同类相食respiration呼吸ingestion摄食digestion消化digestive enzyme消化酶cell细胞nucleus细胞核cytoplasm细胞质plasma lemma / cell membrane细胞膜cell wall细胞壁protein蛋白质amino acid核酸plankton浮游生物heredity遗传mutation of species物种变异chromosome染色体genetic engineering 遗传工程solitary独居social群居bio-diversity生物多样性metamorphosis变态/变形mutation变种variation变异2. 动物学zoology动物学Darwinism达尔文学说natural selection自然选择phylum门class纲order 目suborder亚目family科genus属species 种invertebrate无脊椎动物vertebrate脊椎动物aquatic life 水生动物reptile爬行动物amphibian/amphibious animal两栖动物protozoa原生动物rodent啮齿动物ruminant反刍动物parasitic animal寄生动物primate 灵长动物plankton浮游生物mollusk软体动物coelenterate腔肠动物(如水母、海蜇、珊瑚等)herbivore食草动物mammal哺乳动物homotherm恒温动物cold-blooded animal冷血动物poikilotherm变温动物scavenger食腐动物carnivorous食肉的herbivorous食草的omnivorous杂食的bird鸟类camouflage伪装hibernate冬眠;蛰伏regeneration再生predatory / carnivore食肉的predator捕食者prey捕食hordes/swarms(昆虫等)群flock (牛、羊等)群community动物的群落或人的部落population种群herd兽群hygiene卫生sanitation公共卫生;卫生设施monogamous一夫一妻的/一雌一雄的polygamous一夫多妻的/一雄多雌的polyandrous一妻多夫的/一雌多雄的nomadic游牧的;流浪的trapper诱捕动物者niche小生态环境vestige 退化器fertilizer使受精metabolism新陈代谢breed(名词)品种;(动词)繁殖multiply / reproduce繁殖spawn(鱼、虾、蛙等)孵anatomy解剖学appetite食欲creature生物scales鳞feathers羽毛armor甲spinal cord脊椎digestive system消化系统excretory system排泄系统reproductive system生殖系统circulatory system循环系统respiratory system呼吸系统hormonal system内分泌系统digestive duct消化管esophagus食管stomach胃small intestine小肠large intestine大肠anus肛门digestive gland消化腺salivary gland 唾液腺liver肝gallbladder胆pancreas胰squirrel 松鼠marten貂bat蝙蝠squeak(老鼠等)吱吱otter水獭antelope羚羊gorilla大猩猩chimpanzee黑猩猩baboon狒狒hyena鬣狗moose驼鹿beaver海狸elk麋鹿reindeer驯鹿giraffe长颈鹿rhinoceros犀牛hippo河马sloth树懒slothful懒惰的frog青蛙tadpole蝌蚪salamander蝾螈scorpion蝎子turtle龟lizard蜥蜴chameleon变色龙caymen / crocodile 鳄鱼centipede蜈蚣robin知更鸟owl猫头鹰barnacle北极鹅penguin企鹅canary金丝雀chirp(鸟、虫的叫声)vulture秃鹫crane鹤stork 鹳ptarmigan雷鸟migrate迁移plumage 羽体camouflage伪装wing翅膀bill(鸟)嘴beak(鹰等的)嘴insect昆虫wasp黄蜂hornet 大黄蜂spider蜘蛛pest害虫worm虫/蠕虫cicada蝉mantis螳螂cockroach蟑螂earthworm 蚯蚓antenna / tentacle触须larva幼虫3. 海洋生物学jellyfish水母nettlefish海蜇coral珊瑚dolphin海豚whale鲸鱼shrimp小虾prawn对虾lobster龙虾crab螃蟹mussel贻贝;蚌类clam蛤蜊oyster牡蛎sponge海绵starfish海星squid鱿鱼;乌贼burro / octopus章鱼sole鳎;鳎目鱼plaice鲽,红斑比目鱼4. 植物学botany植物学botanical / botanic植物学的horticulture园艺学aquatic plant水生植物parasite plant寄生植物root根canopy 树冠层/顶棚foliage / leaf叶leaflet小叶rosette(叶的)丛生stem茎stalk杆leafstalk叶柄shoot / sprout嫩芽/抽枝flower 花bud花蕾petal 花瓣peel / skin果皮shell(硬)果壳husk(干)果壳/(玉米)苞叶trunk树干bark 树皮branch树枝bough大或者粗的树枝twig小树枝jungle 丛林lawn草坪meadow草地/牧场prairie 大草原mosses苔藓shrub / bush灌木cluster一簇(灌木)fern蕨类植物horsetails木贼类植物club mosses 石松类植物herb 草photosynthesis 光合作用chlorophyll 叶绿素symbiosis共生symbiotic 共生的wither / shrivel / fade凋谢blossom花pollen花粉pollinate传授花粉petal花瓣nectar花蜜tissue组织organ器官system系统seeds 种子everlasting永久的crossbreed杂交root pressure根压bore腔/肠cohesion-tension凝聚压力column花柱necrosis坏死barren贫瘠的;不生育的futile 无用的carbohydrate (starch) 碳水化合物(淀粉)glucose葡萄糖starch淀粉fat脂肪protein蛋白质vitamin 维他命malnourished 营养不良的nutrition 营养perennial多年一生的annual一年一生的verdant 绿油油的,嫩绿的,翠绿的evergreen常青树conifer tree 针叶树larch落叶松pine松树spruce云杉juniper 刺柏;杜松sequoia红杉elm榆树walnut核桃树redwood 红木树plum blossom梅花orchid兰花chrysanthemum菊花water lily荷花/莲花rhododendron 杜鹃花rose 玫瑰carnation康乃馨lily 百合jasmine茉莉花helianthus / heliotrope / sunflower向日葵camellia茶花corn / maize / mealie玉米pumpkin南瓜tomato 番茄lettuce莴苣cabbage 卷心菜wheat 小麦rye 黑麦barley大麦oats燕麦5. 气象学meteorology气象meteorologist气象学家meteorological station气象站forecast / predict 预报climate气候atmosphere大气层troposphere对流层stratosphere平流层mesosphere 中间层ionosphere电离层exosphere逸散层cold front冷锋warm air mass热气团current(气)流moisture潮湿,水气spell某种天气持续一段时间vapor蒸汽evaporate蒸发damp / moist / humid潮湿humidity 湿度moisture潮湿/ 水分saturate饱和dew 露frost 霜fog / mist 雾smog 烟雾droplet 小水condense浓缩crystal水晶体sheet (水、冰、雪的)一层downpour / torrential rain大雨tempest (storm) / torrential rain暴风雨drizzle细雨shower阵雨hail冰雹blizzard / snowstorm暴风雪avalanche / snow slide 雪崩precipitation(雨、露、雪等)降水thunder 雷breeze微风sandstorm 沙暴monsoon季风gale大风whirlwind 旋风typhoon台风hurricane飓风tornado / twister / cyclone龙卷风wind scale风级tsunami / seismic sea wave海啸tidal wave潮汐;浪潮upper atmosphere上层大气funnel漏斗云disaster / calamity / catastrophe灾难devastation破坏submerge 淹没drought 旱灾convection对流wind velocity风速wind direction 风向long-range forecast长期预报numerical weather prediction数值天气预报nephanalysis云层分析;卫星云图6. 地质学crust地壳mantle 地幔core地核continental crust大陆地壳oceanic crust海洋地壳layer / stratum地层stratigraphy 地层学fault 断层fault plane断层面fault zone断层带rift / crack / split断裂disintegration / decomposition分解erosion腐蚀fossil化石igneous rock 火成岩sedimentary rock沉积岩metamorphic rock 变质岩limestone 石灰岩granite花岗岩marble大理石lithosphere岩石圈magma / molten lava岩浆quartz石英mineral矿物ore矿石deposit矿床rubble 碎石debris残骸platinum白金/铂金silver银copper黄铜aluminum铝tin锡lead 铅zinc锌nickel镍mercury汞/水银sodium 钠gem宝石diamond钻石emerald 绿宝石ruby红宝石glacier 冰川glacial冰川的glacial epoch / age / period冰川期glacial drift冰渍moraines冰碛iceberg冰山volcano 火山active volcano 活火山extinct volcano死火山dormant volcano 休眠火山(sloping) shield volcano盾状火山(平缓)(steep-sided) cone volcano锥状火山(陡峭)eruption火山喷发crater火山口caldera (开口较大的)火山口depression洼地,凹陷处;盆地lava火山岩浆volcanic dust 火山尘volcanic ash火山灰geyser间歇喷泉hot spring 温泉earthquake / quake / tremor / seism地震seismic地震的seismology地震学magnitude震级seismic intensity scale震烈度seismic wave 地震波transverse wave横波longitudinal wave纵波epicenter 震中epicentral distance震中距aftershock 余震cataclysm灾变tsunami / tidal / force海啸undersea landslide 海底山崩melt global warming 全球逐渐变暖aquifer 蓄水层swamp沼泽peat bog泥炭沼泽Great Canyon大峡谷Nile River尼罗河Colorado river 科罗拉多河crumples zones地质缓冲地带bedrock岩床bulge凸起物7. 考古学archaeology考古学paleontology古生物学anthropolog人类学archaeologist人类学家pale-anthropologist古人类学家ecological anthropologist生态人类学家psychological anthropologist心理人类学家originate起源于ancestor祖先hominid 人(科)homogeneous 同以种族(种类)的tribe 部落clan氏族excavation挖掘excavate / unearth挖掘ruins 遗迹/废墟remains遗产/遗骸artifact手工艺品relic遗物/文物antique 古物/古董antiquity古代/古老Stone Age 石器时代Bronze Age 青铜器时代Iron Age铁器时代Paleolithic旧石器时代的Mesolithic 中石器时代的Neolithic新石器时代的morphology形态学skull颅骨cranial颅骨的fossil化石ancient civilization古代文明cave man山顶洞人cultural relics文物rock painting岩画8. 地理学hemisphere 半球meridian 子午线/ 经线parallel 平行圈latitude纬线longitude经线/ 经度elevation海拔altitude高度/ 海拔horizon地平线equator赤道temperature latitudes 温带地区tropics 热带地区Arctic / the North Pole北极Antarctic / Antarctica南极the Antarctic Continent 南极洲the Antarctic Circle南极圈the Arctic Circle北极圈aurora极光tropics of Cancer 北回归线tropics of Capricorn 南回归线international date line国际日期变更线time difference 时差time zone时区topography 地形/ 地形学plain平原plateau / highland 高地lowland 低地basin 盆地oasis绿洲enclave飞地peak山峰cordillera / ranges山脉carven / cave洞穴terrain地域subterranean地底下coastland 沿海地区coastline海岸线watershed分水岭upper reaches上游lower reaches下游tributary 支流deposit沉积spring / fountain泉水iceberg 冰山riverbed河床gulf / bay海湾waterfall 瀑布cascade小瀑布;喷流reef暗礁tide湖水torrent水的急流tropical rain forest热带雨林continental island 大陆岛volcanic island 火山岛coral island珊瑚岛islet小岛peninsular 半岛archipelago群岛delta三角洲landlocked area内陆inland waterway 内陆河subcontinent 次大陆cliff山崖valley山谷hillside / mountain slope山坡continental shelf 大陆架canyon / gorge峡谷channel / strait 海峡remote-sensing遥感的terrestrial 地球的/陆地的terrestrial heat / geothermal 地热terrestrial magnetism 地磁continental drift 大陆漂移学sea-floor spreading 海床扩展evaporation蒸发salinity含盐度ocean bottom 海床sediment沉积物tropical热带的temperate 温带的frigid 寒带的frost heaving 冻胀现象tundra苔原,冻原fieldstone卵石the Mediterranean Sea地中海the primeval forest原始森林Scandinavia斯堪的纳维亚(半岛)(瑞典、挪威、丹麦、冰岛的泛称)fjord峡湾coral reef珊瑚礁Chalk白垩纪cataclysm大洪水ridge山脊;分水岭abyss 深渊territory 版图;领土地域Pyrenees比利牛斯山脉Carpathians喀尔巴阡山脉Vesuvius维苏威火山Pompeii庞贝precipice悬崖eon世;纪;代glacier冰河Pangaea盘古大陆dune 沙丘Lagoon 咸水湖9. 天文学astronomy天文学astronomical observatory天文台planetarium天文馆astrophysics 天文物理学astrology占星学pseudoscience伪科学cosmos / universe 宇宙cosmology 宇宙infinite无限的cosmic宇宙的cosmic radiation宇宙辐射cosmic rays宇宙射线celestial 天体的celestial body / heavenly body天体celestial map / sky atlas天体图celestial sphere 天球dwarf / dwarf star矮星quasar类星体constellation 星座galaxy / Milky Way银河系star cluster星团asterism星群solar system太阳系solar corona日冕solar eclipse日食solar radiation 太阳辐射planet行星planetoid / asteroid 小行星revolve旋转twinkle闪烁naked eye肉眼Mercury水星Venus金星Earth地球Mars火星Jupiter木星Saturn 土星Uranus天王星Neptune 海王星Pluto 冥王星orbit 轨道spin旋转satellite卫星lunar月球的meteor流星meteor shower流星雨star恒星meteoroid流星体meteorite陨石comet 彗星space / outer space太空,外层空间spacecraft / spaceship宇宙飞船space shuttle 航天飞机space telescope空间望远镜astronaut / spaceman 宇航员space suit 宇航服stellar 恒星的intergalactic星系间的interstellar 恒星间的interplanetary行星间的asteroid小行星nebula 星云space debris 太空垃圾ammonia氨photosphere光球;光球层chromospheres 色球;色球层日冕层sunspot太阳黑子(发生在光球层)flare耀斑(发生在色球层)solar prominence日珥(发生在色球层)convection zone对流层vacuum真空infrared ray红外线absolute magnitude绝对量级emission发射/散发high-resolution 高清晰度interferometer 干扰仪,干涉仪illusive object 幻影体faint 微弱的image影像gravitational force吸引力molten融化的leap year闰年rotation 自传revolution公转black hole 黑洞ultraviolet ray紫外线luminosity光度light year光年10. 环保相关ecology 生态学ecosystem生态系统balance of nature自然界生态平衡fauna动物群flora 植物群rain forest雨林food chain 食物链acid rain酸雨greenhouse温室效应infrared radiation红外线辐射ozone layer / ozonosphere臭氧层ultraviolet radiation紫外辐射pollution control污染控制air pollution 空气控制water pollution 水污染noxious / toxic 有毒的fumes(有毒的)废气waste 废物solid waste固体废物sewage / wastewater 污水sewage purification污水净化swage disposal污水处理decibel(噪音)分贝11. 能源相关fossil fuel矿物燃料process of photo synthesis光合作用solar energy太阳能nonrenewable 不可再生的energy conservation保护能源resource资源energy source能源资源tidal energy 潮汐能fuel-efficient节能型的rush hour高峰期zero emission零辐射wildness野生/天然preservation保护atmosphere大气carbon碳dioxide 二氧化物burning of coal and oil煤油燃烧global warming全球变暖greenhouse effect温室效应rise in sea level海平面上升long-term climatic change长期的气候变化environmental recycling center再循环利用中心litter/trash garbage垃圾pollutant污染物desertification沙漠化deforest 滥伐森林drought干旱water shortage 水源缺乏offshore spillage 近海岸溢出carbon dioxide release 二氧化碳排放industrial sewage工业污水recycling再循环purify 净化deteriorate恶化acid rain酸雨sewage disposal污水处理environment protection环境保护ozone layer臭氧层waste disposal废物处理emission(汽车废气的)排放soot烟尘El Niño 厄尔尼诺现象12. 新技术发明相关13. 人类学artist艺术家choreographer舞蹈编排家critic 批评家satirist讽刺作家inventor发明家biographer自传作家sculptor雕塑家feminist 女权主义者humanitarian人道主义者imagist 意象派诗人philanthropist 慈善家proprietor业主mortal 犯人precursor先驱figurehead 名誉领袖disciple 学徒apprentice学徒mechanic机械工minimalist简单抽象派艺术家avant-garde前卫派territory领域genre风格/体裁eccentric古怪的odd怪诞的/奇数的erratic奇怪的weird怪异的/不可思议的romantic浪漫的innocent天真的/无罪的lovelorn相思病苦的emotional情绪的/情感的sentimental感伤的/多愁善感的cheerless无精打采的/无生命力的patriarchal 家长的/族长的rigid僵化的spare简朴的clumsy笨拙的zigzag曲折的contemporary当代的acclaimed受欢迎的preeminent 杰出的versatile (人)多才多艺的/(物)多功能的household家庭的/家喻户晓的genuine真正的authentic 逼真的/原汁原味的symbolic象征性的immortal不朽的/神nostalgia怀旧主义/思乡emotive 感人的prodigious巨大的classic 经典的posthumous死后的14. 发展史文学pose散文diary日记autobiography 传记editorial 社论narrative prose叙述性descriptive prose 描写性essay随笔poetry 诗歌ballad民谣lullaby催眠曲fiction小说allegory寓言fairy tale童话legend传说proverb 谚语model人物原型leading character主人公main plot主要情节prelude序曲prologue序言epilogue 尾声literary criticism文学批评literary studies文学研究schools of literature文学流派comparative literature比较文学realism现实主义surrealism超现实主义futurism未来主义modernism现代主义aestheticism唯美主义音乐musical instrument乐器orchestra(管弦)乐队shook rattle摇拨浪鼓pound dru 击鼓foot beat跺脚note 音符score乐谱movement 乐章fanatical狂热的hillbilly music乡村音乐folk music民间音乐pop music流行音乐classical music古典音乐Jazz爵士乐symphony交响乐rock and roll摇滚乐band music / wind music管乐string弦乐violin小提琴viola中提琴cello大提琴harp 竖琴horn号;喇叭clarinet单簧管oboe双簧管keyboard instrument键盘乐器percussion震荡/打击乐器vocal music声乐concerto协奏曲sonata奏鸣曲serenade小夜曲solo独奏/独唱duet二重唱conducting指挥podium指挥台accompaniment伴奏quality音质volume音量chord和弦harmony和声rest 休止time节拍lullaby催眠曲prelude 序曲epilogue尾声政治经济subsistence存活,生活subsistence wage刚够养家糊口的工资subsistence level 收支平衡的生活水平kinship亲属关系,血缘关系commodity商品check支票;收据;账单bank loan 银行贷款interest 利息withdraw从银行账户中提款honor (a cheque/bill/draft)承兑a run on a bank挤兑the Great Depression大萧条consumerism消费主义(认为高消费对个人和社会有利的看法);保护消费者权益主义affluence富裕,富足sophisticated世故的,老练的;复杂的,尖端的建筑architecture建筑学architect建筑学家construct结构wing 辐楼/侧楼design设计elevator 电梯skyscraper摩天大楼design element设计元素log structure原木结构cabin小木屋beam梁prototype原型building technique建筑工艺new material新材料metal-frame金属结构repair person修理工planetarium天文馆aquarium水族馆archives档案馆office building 写字楼cathedral大教堂mosque清真寺the statue of liberty自由女神像the triumphal arch凯旋门sphinx狮身人面像pyramid 金字塔castle城堡美术fine arts美术oil painting油画water color水彩画tempera 蛋彩画sketch速写/素描pastel 彩色蜡笔画poster海报/招贴画charcoal drawing木炭画mural painting / fresco壁画engraving版画lithograph石板画landscape painting风景画still life静物画portrait肖像画caricature漫画pigment颜色,色素canvas油画布brush 画笔drawing board画板perspective透视画法original 原作copy临本reproduction / replica复制品genuine真的fake假的gallery美术馆autograph真迹panorama 全景画calligraphy书法paste裱糊impressionistic style印象派风格framing装框sculpture 雕塑sculptor 雕塑家figurine小雕像bust 半身雕塑像statue塑像unique唯一的/独特的animator漫画家saxophonist 萨克斯风管吹奏者indigo靛蓝purple紫色。
抗病和感病香蕉品种根系内生细菌群落结构与多样性

·365·抗病和感病香蕉品种根系内生细菌群落结构与多样性张艳1,曾凤花1,农倩1,覃丽萍1,窦同心2,邱美莎1,谢玲1*(1广西农业科学院植物保护研究所/农业农村部华南果蔬绿色防控重点实验室/广西作物病虫害生物学重点实验室,广西南宁530007;2广东农业科学院果树研究所,广东广州510640)摘要:【目的】分析抗病和感病香蕉品种根系内生细菌群落结构与多样性,探究香蕉抗枯萎病能力与香蕉根系微生物组的关联,为发掘利用香蕉枯萎病土著生防微生物组提供理论依据。
【方法】以香蕉枯萎病感病品种威廉斯B6和抗病品种中蕉9号为材料,在枯萎病发病初期(营养生长旺盛期)和发病严重期(孕蕾期)采集香蕉根系,采样前均调查香蕉发病情况;分别提取抗病和感病香蕉植株根系DNA ,利用高通量测序技术对香蕉根系内生细菌16S rRNA 基因V3~V4区进行测序分析,通过Fastp 和Flash 软件对原始测序序列进行质控、拼接,利用RDP classifier 和Silva 数据库对序列进行比对、注释,采用Excel 2010对数据进行整理统计,运用DPS 7.0进行差异显著性分析。
【结果】随着香蕉的生长发育和枯萎病的越发严重,抗病品种中蕉9号在生长过程中相对感病品种威廉斯B6表现出极显著(P <0.01)的抗病能力。
测序获得的2568个OTUs 分属于32门89纲220目369科673属1139种。
Alpha 多样性和物种组成分析结果表明,抗病品种中蕉9号根系细菌多样性高于感病品种威廉斯B6,但差异不显著(P >0.05)。
2个品种根系细菌群落在发病初期和严重期的优势菌门无差异,但相对丰度随着香蕉的生长发生显著变化(P <0.05,下同)。
在发病初期,感病和抗病香蕉植株根系细菌群落中主要优势菌属较单一;发病严重期细菌群落组成较丰富,其中抗病品种中有益微生物链霉菌属(Streptomyces )、拟无枝菌酸菌属(Amycolatopsis )和短杆菌属(Brevibacterium )的相对丰度显著增加,分析发现短杆菌属只在抗病品种香蕉根系中特异富集。
An increasing threat in hospitals_ multidrug-resistant Acinetobacter baumannii.

Intensive care units (ICUs) of hospitals harbour critically ill patients who are extremely vulnerable to infections. These units, and their patients, pro-vide a niche for opportunistic microorganisms that are generally harmless for healthy individuals but that are often highly resistant to antibiotics and can spread epidemically among patients. Infections by such organisms are difficult to treat and can lead to an increase in morbidity and mortality. Furthermore, their eradication from the hospital environment can require targeted measures, such as the isolation of patients and temporary closure or even reconstruction of wards. The presence of these organisms, therefore, poses both a medical and an organizational burden to health-care facilities.One important group of bacteria that is associated with these problems is the heterogeneous group of organisms that belong to the genus Acinetobacter. This genus has a complex taxonomic history. Since the 1980s, in parallel with the emergence of acinetobacters as noso-comial pathogens, the taxonomy of the genus has been refined; 17 named species have been recognized and 15 genomic species (gen.sp.) have been delineated by DNA–DNA hybridization, but these do not yet have valid names (TABLE 1). The species that is most commonly involved in hospital infection is Acinetobacter bauman-nii, which causes a wide range of infections, including pneumonia and blood-stream infections. Numerous studies have reported the occurrence of multidrug-resistant (MDR) A. baumannii in hospitals, and at some locations pandrug-resistant strains have been identified. Currently, A. baumannii ranks among the most important nosocomial pathogens. Additionally, the number of reports of community-acquired A. baumannii infection has been steadily increasing, although overall this type of infection remains rare. Despite the numerous publi-cations that have commented on the epidemic spread of A. baumannii, little is known about the mechanisms that have favoured the evolution of this organism to multi-drug resistance and epidemicity. In this Review, we dis-cuss the current state of knowledge of the epidemiology, antimicrobial resistance and clinical significance of acinetobacters, with an emphasis on A. baumannii. The reader is also referred to previous reviews of this organism that have been written by pioneers in the field1,2.Identification of Acinetobacter speciesIn 1986, a phenotypic system for the identification of Acinetobacter species was described3, which together with a subsequent simplified version4 has proven useful for the identification of most, but not all, Acinetobacter species. In particular, Acinetobacter calcoaceticus, A. baumannii, gen.sp. 3 and gen.sp. 13TU cannot be separated well by this system4. These species are also highly similar by DNA–DNA hybridization5 and it has therefore been proposed that they should be grouped together into the so-called A. calcoaceticus–A. baumannii (Acb) complex4. From a clinical perspective this might not be appropriate, as the complex combines three of the most clinically relevant species (A. baumannii, gen.sp. 3 and gen.sp. 13TU) with an environmental spe-cies (A. calcoaceticus). It is noteworthy that the perform-ance of commercial systems for species identification that are used in diagnostic microbiology is also unsatisfactory.*Department of Infectious Diseases C5‑P, Leiden University Medical Centre, Albinusdreef 2, P.O.BOX 9600, 2300 RC Leiden, the Netherlands.‡Centre of Epidemiology and Microbiology, National Institute of Public Health, Srobarova 48, 10042 Prague, Czech Republic.§Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Goldenfelsstrasse 19‑21, 50935 Cologne, Germany. Correspondence to L.D.e‑mail: l.dijkshoorn@lumc.nl doi:10.1038/nrmicro1789An increasing threat in hospitals: multidrug-resistant Acinetobacter baumanniiLenie Dijkshoorn*, Alexandr Nemec‡ and Harald Seifert§Abstract | Since the 1970s, the spread of multidrug-resistant (MDR) Acinetobacter strains among critically ill, hospitalized patients, and subsequent epidemics, have become an increasing cause of concern. Reports of community-acquired Acinetobacter infections have also increased over the past decade. A recent manifestation of MDR Acinetobacter that has attracted public attention is its association with infections in severely injured soldiers. Here, we present an overview of the current knowledge of the genus Acinetobacter, with the emphasis on the clinically most important species, Acinetobacter baumannii.DNA–DNA hybridizationDetermines the degree ofsimilarity between the genomicDNA of two bacterial strains;the gold standard to assesswhether organisms belong tothe same species.Pandrug-resistantIn this Review, refers toA. baumannii that are resistantto all available systemicanti-A. baumannii antimicrobialagents, except for polymyxins.NATURe RevIewS |microbiology vOlUMe 5 | DeCeMbeR 2007 |939©2007Nature Publishing GroupIsolateA population of bacterial cells in pure culture that is derived from a single ing these systems, the clinically relevant species ofthe Acb complex are frequently uniformly identified asA. baumannii and many other species are not identified6–8.These problems have led to the development of genotypicmethods for Acinetobacter species identification, some ofwhich are discussed in BOX 1(also see fIg. 1). Currently,precise species identification is not feasible in most labora-tories, except for a few Acinetobacter reference laboratories.In light of the difficulties in distinguishing A. baumannii,gen.sp. 3 and gen.sp. 13TU, in this Review these specieswill be referred to as A. baumannii (in a broad sense)unless otherwise stated.Epidemiology of clinical acinetobactersThe natural habitat of Acinetobacter species.MostAcinetobacter species have been found in clinicalspecimens (TABLE 1), but not all are considered to beclinically significant. One important question is wheredoes A. baumannii come from? Furthermore, are thereenvironmental or community reservoirs? As mentionedearlier, A. baumannii, gen.sp. 3 and gen.sp. 13TU are themost frequent species that are found in human clinicalspecimens5,9,10. Of these, gen.sp. 3 was the most prevalentspecies among clinical isolates in a Swedish study5. In2 european studies, Acinetobacter lwoffii was the most Table 1 | Classification of the genusAcinetobacterto label these species by the initials of their respective authors, Tjernberg and Ursing (TU)5 or Bouvet and Jeanjean (BJ)108.940 | DeCeMbeR 2007 | vOlUMe 5 /reviews/micro©2007Nature Publishing GroupNature Reviews | Microbiology predominant species to be found on the skin of healthy individuals, with carrier rates of 29% and 58%, whereas other Acinetobacter species, including Acinetobacter junii , Acinetobacter johnsonii , Acinetobacter radioresistens and gen.sp. 15bJ, were detected at lower frequencies 11,12. The carrier rates for A. baumannii (including gen.sp. 13TU) in these studies ranged from 0.5 to 3%, whereas for gen.sp. 3 the rates ranged from 2 to 6%11,12. The faecal carriage of A. baumannii among non-hospitalized individuals in the United Kingdom and the Netherlands was 0.9%13. The most predominant species in faecal samples from the Netherlands were A. johnsonii (17.5%) and gen.sp. 11 (4%)13. A. baumannii was also recovered from the bodylice of homeless people 14 and it was proposed that the organisms were associated with transient bacteraemia in these individuals. In a study in Hong Kong, the car-rier rates of A. baumannii , gen.sp. 3 and gen.sp. 13TU on the skin of healthy individuals were 4, 32 and 14%, respectively 15. Thus, the carrier rates for gen.sp. 3 and gen.sp. 13TU in that study were strikingly higher than in the european studies. These findings indicate that, at least in europe, the carriage of A. baumannii in the community is relatively low. Apart from its occurrence in humans, A. baumannii has also been associated with infection and epidemic spread in animals at aveterinary clinic 16.NATURe RevIewS | microbiologyvOlUMe 5 | DeCeMbeR 2007 | 941© 2007Nature Publishing Group908070605040302010100Acinetobacter grimontiiGen.sp. 15TUAcinetobacter junii Acinetobacter haemolyticus Gen.sp. 14BJGen.sp. 13TUA. baumannii Gen.sp. 3Gen.sp.‘close to 13TU’Acinetobacter venetianusAcinetobacter calcoaceticusGen. sp.‘between 1 and 3’Acinetobacter tjernbergiaeAcinetobacter towneriAcinetobacter ursingiiGen.sp. 13BJ or 14TU Gen.sp. 15BJ Gen.sp. 17A. baylyiAcinetobacter lwoffiiAcinetobacter schindleriAcinetobacter bouvetiiAcinetobacter gerneriGen.sp. 10Gen.sp. 11Acinetobacter johnsoniiAcinetobacter radioresistens Acinetobacter parvusPearson correlation Species Gen.sp. 16Gen.sp. 6Nature Reviews | Microbiology Acinetobacter tandoii EndemicThe constant presence of aninfectious agent in a givengeographical area or hospital.There are few available data on the environmental occurrence of A. baumannii , gen.sp. 3 and gen.sp. 13TU,but these species have been found in varying percent-ages in vegetables, fish, meat and soil 17,18. A. baumannii has also recently been found in aquacultures of fish and shrimp farms in Southeast Asia 19. However, it is not yet clear to what extent these findings are attributable to an environmental niche or to contact with humans or animals. A. baumannii has been described as a soil organism, but without the support of appropriate references 20. It was probably assumed that the wide occurrence of unspeciated acinetobacters in soil and water 21 is also applicable to A. baumannii . However, in fact, there is little evidence that A. baumannii is a typical soil resident. Taken together, the existing data indicate that A. baumannii has a low prevalence in the community and that its occurrence in the environment is rare.A. baumannii in hospitals. The most striking mani-festation of A. baumannii is the endemic and epidemic occurrence of MDR strains in hospitals. The closely related gen.sp. 3 and gen.sp. 13TU might have a similar role 22–24, and their involvement could have been under-estimated as these species are phenotypically difficult to discriminate from A. baumannii . Most investigations ofA. baumannii in hospitals have been ad hoc studies thatwere triggered by an outbreak. More in-depth studies of the prevalence of this species in hospitals, including antibiotic-resistant and antibiotic-susceptible strains, are required to better understand its true importance.Depending on the local circumstances, and the strain in question, the pattern of an outbreak can vary. Therecan be a common source or multiple sources and somestrains have a greater tendency for epidemic spread than others. epidemiological typing — mostly by genotypic methods, such as amplified fragment length polymor-phism (AFlP) analysis (BOX 1) — is an important tool that can distinguish an outbreak strain from other, concurrent strains, and assess the sources and mode oftransmission of the outbreak strain.A scheme that depicts the dynamics of epidemic A. baumannii on a hospital ward is provided in fIg. 2. An epidemic strain is most commonly introduced by apatient who is colonized. Once on a ward, the strain can then spread to other patients and their environment. A. baumannii can survive in dry conditions 25 and during outbreaks has been recovered from various sites in thepatients’ environment, including bed curtains, furnitureand hospital equipment 26. These observations, and thesuccess that cleaning and disinfecting patients’ rooms has had in halting outbreaks, emphasize the role of thehospital environment as a reservoir for A. baumanniiduring outbreaks. The bacteria can be spread throughthe air over short distances in water droplets and inscales of skin from patients who are colonized 27, but the most common mode of transmission is from the handsof hospital staff. Patients who are colonized or infected by a particular A. baumannii strain can carry this strain at different body sites for periods of days to weeks 28, and colonization can go unnoticed if the epidemic strain isnot detected in clinical specimens 2,29.Population studies of A. baumannii . Comparative typ-ing of epidemic strains from different hospitals has indicated that there can be spread between hospitals. For example, during a period of outbreaks in the Netherlands that involved eight hospitals, one common strain was found in three of these hospitals and another common strain was found in two others 26. Similar observations of interhospital spread of MDR strains in particular geographical areas have been made in the Czech Republic 30, the United Kingdom 31, Portugal 32and the United States 33. Highly similar, but distinguishable, strains have been found at different locations and at different time points,Figure 1 | Amplified fragment length polymorphism (AFlP) analysis of Acinetobacter strains. A condensed dendrogram of the AFLP (described in BOX 1)fingerprints of 267 Acinetobacter reference strains of 32 described genomic species. Allspecies are well separated at the 50% cluster-cut-off level, which emphasizes the powerof this method for the delineation and identification of Acinetobacter species.942 | DeCeMbeR 2007 | vOlUMe 5/reviews/micro© 2007Nature Publishing GroupNature Reviews |MicrobiologyClonesA group of bacteria that wereisolated independently fromdifferent sources in time andspace, but share so manyidentical traits that it is likelythat they evolved from acommon ancestor.T yping methodA tool that differentiatesbacterial strains below the species level.RibotypingA typing method in which chromosomal DNA is digested by restriction enzymes, fragments are separated by electrophoresis and, finally, particular fragments are detected by labelled rRNA probes to generate DNA-banding patterns, which allows the differentiation of bacterial isolates.without a direct epidemiological link. It is assumed thatthese strains represent particular lineages of descent(clones). examples are european clones I–III34–36, whichhave been delineated by a range of genotypic typing meth-ods, such as AFlP analysis (BOX 1a; fIg. 1), ribotyping,macrorestriction analysis by pulsed-field gel electrophore-sis and, most recently, multilocus sequence typing (seeboth MlST systems in Further information). Strainsthat belong to these clones are usually highly resistantto antibiotics, although within a clone there can bevariation in antibiotic susceptibility. Apparently, theseclones are genetically stable strains that are particularlysuccessful in the hospital environment and evolveslowly during their spread. whether these strains haveparticular virulence attributes or an enhanced ability tocolonize particular patients (discussed below) remainsto be established. Their wide spread might be explainedby the transfer of patients between hospitals and regionsover the course of time, although in many cases there isno evidence for this. It is also possible that they circulateat low rates in the community and are able to expand inhospitals under selective pressure from antibiotics. Sofar, their resistance to antimicrobial agents is the onlyknown selectively advantageous trait.Figure 2 | overview of the dynamics between patients, bacteria and the hospital environment. The possible modes of Acinetobacter baumannii entry into a ward are shown. Entrance through a colonized patient is the most likely mode. However, introduction through contaminated materials (such as pillows104) has also been documented. Notably, introduction by healthy carriers is also conceivable, although it is not known whether the rare strains that circulate inthe community have epidemic potential. Once on a ward, A. baumannii can spread from the colonized patient to the environment and other susceptible patients. The direct environment of the patient can become contaminated by excreta, air droplets and scales of skin. Interestingly, A. baumannii can survive well in the dry environment25, a feature it shares with staphylococci. Hence, the contaminated environment can become a reservoir from which the organism can spread. The acquisition of A. baumannii by susceptible patients can occur through various routes, of which the hands of hospital staff are thought to be the most common, although the precise mode of transmission is usually difficult to assess.NATURe RevIewS |microbiology vOlUMe 5 | DeCeMbeR 2007 |943©2007Nature Publishing GroupMacrorestriction analysisA typing method in which chromosomal DNA is digested with rare-cutting enzymes, so creating large fragments that are separated in an alternating electric field (pulsed-field electrophoresis) according to their size.OsteomyelitisAn infection of bone or bone marrow.Clinical impact of Acinetobacter infectionsNosocomial infections. Acinetobacters are opportun-istic pathogens that have been implicated in variousinfections that mainly affect critically ill patients inICUs. Hospital-acquired Acinetobacter spp.infectionsinclude: ventilator-associated pneumonia; skin andsoft-tissue infections; wound infections; urinary-tractinfections; secondary meningitis; and bloodstreaminfections. These infections are mainly attributed toA. baumannii, although gen.sp. 3 and gen.sp. 13TUhave also been implicated. Nosocomial infectionsthat are caused by other Acinetobacter species, suchas A. johnsonii, A. junii, A. lwoffii, Acinetobacterparvus, A. radioresistens, Acinetobacter schindleriand Acinetobacter ursingii, are rare and are mainlyrestricted to catheter-related bloodstream infec-tions8,37–40. These infections cause minimal mortalityand their clinical course is usually benign, althoughlife-threatening sepsis has been observed occasion-ally41. The rare outbreaks of some of these species (forexample, A. junii) have been found to be related tocontaminated infusion fluids41.The risk factors that predispose individuals to theacquisition of, and infection with, A. baumannii aresimilar to those that have been identified for otherMDR organisms. These include: host factors suchas major surgery, major trauma (in particular, burntrauma) and prematurity in newborns; exposure-related factors such as a previous stay in an ICU, thelength of stay in a hospital or ICU, residence in a unitin which A. baumannii is endemic and exposure tocontaminated medical equipment; and factors thatare related to medical treatment such as mechani-cal ventilation, the presence of indwelling devices(such as intravascular catheters, urinary cathetersand drainage tubes), the number of invasive proce-dures that are performed and previous antimicrobialtherapy42. Risk factors that are specific for a par-ticular setting have also been identified, such as thehydrotherapy that is used to treat burn patients andthe pulsatile lavage treatment that is used for wounddébridement43,44.The most frequent clinical manifestations of noso-comial A. baumannii infection are ventilator-associatedpneumonia and bloodstream infection, both of whichare associated with considerable morbidity and mor-tality, which can be as high as 52%45,46. Risk factorsfor a fatal outcome are severity-of-illness markers, anultimately fatal underlying disease and septic shockat the onset of infection. bacteraemic A. baumanniipneumonia has a particularly poor prognosis46. Acharacteristic clinical manifestation is cerebrospinal-shunt-related meningitis, caused by A. baumannii inpatients who have had neurosurgery47. wound infec-tions have been reported mainly in patients who havesevere burns or trauma, for example, soldiers who havebeen injured during military operations43,48. Urinary-tract infections related to indwelling urinary-tractcatheters usually run a more benign clinical courseand are more frequent in rehabilitation centres thanin ICUs49.The clinical impact of nosocomial A. baumanniiinfection has been a matter of continuing debate. Manystudies report high overall mortality rates in patientsthat have A. baumannii bacteraemia or pneumonia45,46.However, A. baumannii mainly affects patients withsevere underlying disease and a poor prognosis. It hastherefore been argued that the mortality that is observedin patients with A. baumannii infections is caused bytheir underlying disease, rather than as a consequenceof A. baumannii infection. In a case-control study, blotand colleagues50 addressed whether A. baumannii con-tributes independently to mortality and concluded thatA. baumannii bacteraemia is not associated with a sig-nificant increase in attributable mortality. Similar find-ings for A. baumannii pneumonia have been reportedby Garnacho and colleagues51. by contrast, in recentreviews of matched cohort and case-control studies,Falagas and colleagues52,53 concluded that A. baumanniiinfection was associated with an increase in attributablemortality, ranging from 7.8 to 23%. These contradictoryconclusions show that the debate on the clinical impactof A. baumannii is still ongoing.Community-acquired infections.A. baumannii isincreasingly recognized as an uncommon but impor-tant cause of community-acquired pneumonia. Mostof the reported cases have been associated withunderlying conditions, such as alcoholism, smoking,chronic obstructive pulmonary disease and diabetesmellitus. Community-acquired A. baumannii pneu-monia appears to be a unique clinical entity thathas a high incidence of bacteraemia, a fulminantclinical course and a high mortality that ranges from40 to 64%. It has been observed almost exclusivelyin tropical climates, in particular in Southeast Asiaand tropical Australia54,55. It is currently unclear, how-ever, if host factors or particular virulence factors areresponsible for these severe infections. Multidrugresistance in these organisms is uncommon55. Othermanifestations of community-acquired A. baumanniiinfections are rare.Infections associated with natural disasters and warcasualties. A characteristic manifestation of nosoco-mial A. baumannii is wound infection that is associ-ated with natural or man-made disasters, such as theMarmara earthquake that occurred in 1999 in Turkey,the 2002 bali bombing and military operations48,56,57.A strikingly high number of deep-wound infections,burn-wound infections and osteomyelitis cases havebeen reported to be associated with repatriated casual-ties of the Iraq conflict48. Isolates often had multidrugresistance. based on the common misconception thatA. baumannii is ubiquitous, it has been argued that theorganism might have been inoculated at the time ofinjury, either from previously colonized skin or fromcontaminated soil. However, recent data clearly indi-cate that contamination of the environment of fieldhospitals and infection transmission in health-carefacilities have had a major role in the acquisition ofA. baumannii58.944 | DeCeMbeR 2007 | vOlUMe 5 /reviews/micro©2007Nature Publishing GroupNature Reviews |MicrobiologyQuorum sensingThe phenomenon whereby the accumulation of signalling molecules enables a single cell to sense the number of bacteria (cell density) that are present, which allows bacteria to coordinate certain behaviours or actions.Epidemicity and pathogenicityThe fact that colonization with A. baumannii is morecommon than infection, even in susceptible patients,emphasizes that the pathogenicity of this species is gen-erally low. However, once an infection develops, it canbe severe. Studies on the epidemicity and pathogenicityfactors of A. baumannii are still at an elementary stage.A number of putative mechanisms that might have a rolein colonization, infection and epidemic spread are sum-marized in fIg. 3. Genetic, molecular and experimentalstudies are required to elucidate these mechanisms inmore detail.Recent DNA sequencing of a single A. baumanniistrain identified 16 genomic islands that carry putativevirulence genes that are associated with, for example,cell-envelope biogenesis, antibiotic resistance, autoin-ducer production, pilus biogenesis and lipid metabo-lism59. Resistance to desiccation, disinfectants25,60 andantibiotics is important for environmental survival. Theextraordinary metabolic versatility3 of A. baumanniicould contribute to its proliferation on a ward and inpatients. Pilus-mediated biofilm formation on glass andplastics has been demonstrated61. If formed on medicaldevices, such as endotracheal tubes or intravascularcatheters, these biofilms would probably provide a nichefor the bacteria, from which they might colonize patientsand give rise to respiratory-tract or bloodstream infec-tions. electron microscopy studies have demonstratedthat pili on the surface of acinetobacters interact withhuman epithelial cells62. In addition, thread-like connec-tions between these bacteria were suggestive of an earlyphase of biofilm formation. The pili and hydrophobicsugars in the O-side-chain moiety of lipopolysaccharide(lPS)63 might promote adherence to host cells as a firststep in the colonization of patients. Quorum sensing, thepresence of which has been inferred from the detectionof a gene that is involved in autoinducer production59,could control the various metabolic processes, includingbiofilm formation.Resistance to antibiotics, as well as the protectiveconditions of the skin (such as dryness, low pH, theresident normal flora and toxic lipids) and those ofthe mucous membranes (such as the presence of mucus,lactoferrin and lactoperoxidase and the sloughing ofcells) are prerequisites for bacterial survival in a host thatis receiving antibiotics. In vitro and animal experimentshave identified various factors that could have a rolein A. baumannii infection. For example, A. baumanniiouter membrane protein A (AbOmpA, previouslycalled Omp38) has been associated with the inductionof cytotoxicity64. Iron-acquisition mechanisms65 andserum resistance66 are attributes that enable the organ-ism to survive in the bloodstream. The lPS and lipid Aof one strain, at the time named A. calcoaceticus, hadbiological activities in animals that were similar to thoseof other enterobacteria67. These included lethal toxicity,pyrogenicity and mitogenicity for mouse-spleen b cells.More recently, A. baumannii lPS was found to be themajor immunostimulatory component that leads toa proinflammatory response during A. baumanniipneumonia68 in a mouse model.Taken together, the chain of events from environmen-tal presence to the colonization and infection of patientsdemonstrates the extraordinary ability of A. baumannii toadapt to variable conditions. This ability suggests that theorganism must possess, in addition to other factors, effec-tive stress-response mechanisms. Together with its resist-ance to antibiotics, these mechanisms might explain thesuccess of particular A. baumannii strains in hospitals.Figure 3 | The factors that contribute to Acinetobacter baumannii environmentalpersistence and host infection and colonization. Adherence to host cells, asdemonstrated in an in vitro model using bronchial epithelial cells62, is considered to be afirst step in the colonization process. Survival and growth on host skin and mucosalsurfaces require that the organisms can resist antibiotics and inhibitory agents and theconditions that are exerted by these surfaces. Outgrowth on mucosal surfaces andmedical devices, such as intravascular catheters and endotracheal tubes61, can result inbiofilm formation, which enhances the risk of infection of the bloodstream and airways.Quorum sensing59 might have a regulatory role in biofilm formation. Experimentalstudies have identified various factors that could have a role in A. baumannii infection, forexample, lipopolysaccharide has been shown to elicit a proinflammatory response inanimal models67,68. Furthermore, the A. baumannii outer membrane protein A has beendemonstrated to cause cell death in vitro64. Iron-acquisition mechanisms65 and resistanceto the bactericidal activity of human serum66 are considered to be important for survivalin the blood during bloodstream infections. Environmental survival and growth requireattributes such as resistance to desiccation25,60, versatility in growth requirements3, biofilm-forming capacity61 and, probably, quorum-sensing activity59. Finally, adequate stress-response mechanisms are thought to be required for adaptation to different conditions.NATURe RevIewS |microbiology vOlUMe 5 | DeCeMbeR 2007 |945©2007Nature Publishing Group。
基于深度学习的松材线虫病树检测算法研究

47基于深度学习的松材线虫病树检测算法研究叶莎1,丁峰2,彭宜生1(1.三峡大学计算机与信息学院,湖北宜昌443002;2.宜昌三峡大老岭自然保护区管理局,湖北宜昌443000;)摘要:松材线虫病是一种毁灭性森林病害,具有极强的传染性,松材线虫病树的有效防治对森林资源保护具有重要意义。
采用无人机获取森林中松树的航拍正射影像,同时基于深度学习算法对松材线虫病树进行检测是对松材线虫病进行监测、治理最为有效的途径。
文章基于深度学习技术,使用不同的目标检测算法在松材线虫病树识别上的应用进行总结,展示采用不同的目标检测算法在松材线虫病树识别上取得的效果,并分析总结了不同的目标检测算法在松材线虫病树上应用存在的问题。
关键词:松材线虫病;森林资源保护;深度学习技术;目标检测中图分类号:TP309文献标识码:A 文章编号:2096-9759(2023)03-0047-03Research on detection algorithm of pine wilt disease tree based on depth learningYE Sha 1,DING Feng 2,PENG Yisheng 1(1.College of Computer and Information Technology ,China Three Gorges University,Yichang Hubei 443002;2.Yichang Three Gorges Dalaoling Nature Reserve Administration,Yichang,Hubei 443000)Abstract:Pine wilt disease is a destructive forest disease with strong infectivity.Effective control of pine wood nematode dis-ease trees is of great significance to the protection of forest resources.The most effective way to monitor and control pine wood nematode disease is to use UA V to obtain aerial orthophoto images of pine trees in the forest and detect pine wood nematode disease trees based on depth learning algorithm.Based on the deep learning technology,this paper summarizes the application of different target detection algorithms in the identification of pine wood nematode diseased trees,shows the effects of different target detection algorithms in the identification of pine wood nematode diseased trees,and analyzes and summarizes the prob-lems of different target detection algorithms in the application of pine wood nematode diseased trees.Keywords:pine wilt disease;protection of forest resources;deep learning technology;object detection0引言森林资源是我国的重要资源,松材线虫病是一种由松材线虫所引发的松树传染病,它具有传染速度快、防治困难、破坏性强的特性,通常松树染病后最快40天即可造成整株松树枯死,3-5年即可摧毁整片森林,对我国生态环境和经济造成了严重损失[1]。
辣椒细胞质雄性不育系及其保持系的AFLP分析英文

A FL P A n a lys is o f C y top la sm ic M a le 2s te rile L in e a n d Its M a i n ta i n e r L i n e o f P ep p e rLUO Xia ng 2d o n g1,2,DA I L ia n g 2fa n g1,2,CH EN J i n 2fen g23,WAN G S h u 2b i n31.C o ll ege o f Ho rti cu l tu re,N an ji ng Ag ri cu lt u ra lU n i vers it y,N an ji ng 210095;2.Co ll eg e o fL i fe Sc i ence s,J i an gxi No r m al Un i ve rs i ty,N ancha ng 330022;3.Vege tab l e R es ea rch I n s ti tu t e ,J i an gs u Academ y o f Ag ricu lt u ra l Sc i ence s ,N an j ing 210014Ab s t ra ct [O bject i ve]The a i m o f t h is study wa s t o ana l yze the cyt o p l a s m i c m a l e s teri le l i n e 21A and its m ainta i ne r li n e 21B o f p ep pe rs by AF L P,an d l ay t he fo unda ti on fo r further s tudies o n m o l ecu l a r m echan is m o f t he cyt op l a sm i c m ale s te ril ity i n p epp ers .[M ethod ]C yt op l a sm i c m a l e s teril it y (CMS)li ne 21A and its m a i n t a i ne r li ne 21B w ere a nalyzed by AFLP t o o bta i n the s p ecific am p li fied fra gm en ts o f cyt op lasm i c m a l e s teri le li ne 21A,wh i le the sp ecifi c amp li fi ed fragm en ts w ere recove red o r sequ enced,and ana l yzed by BLAS Tn and TBLASTX i n GenB a nk o f NCB I .[Result ]A sp eci fi c fragm en t AA /CAG 169w as ob tained from cytop l am i c m al e ste rili ty (C M S )li ne 21A.Pa rti a l DNA s equen ce of AA /CAG 169w as t he se ries repe ated s equen ce i n AFLP m a rker seq uence related t o the sex and grow t h tra i t s o f Pen aeu s m ono do n.The re su l ts o f BLASTX and TBLASTX showed t ha t the tran s l a ti o n p r o duct s o f t h is sp eci fi c fragm ent had a hi g he r si m il a rity to So l anum dem is sum pu t a ti ve gag 2po l p ro tei n ,So l anum dem i s sum p u t a ti ve retro tra nspo so n p r o te i n and So l a num d em is sum p uta ti ve re tro tran spo so n gag p ro t e i n re s p ec ti vel y.[C on clu si on]The functi o n o f this spec i fic fragem ent AA /CAG 169is mo re li ke l y t o be cl o s el y rel a ted t o re tr o transp o son,wh i ch i nd i cate s tha t the sp eci a l fragm en t is po ss i b l y re l a t e d t o the s te ril ity trait .Key w o rds Pep pe r;cy t op l a s m i c m ale 2s t e ri lti y (C M S);AFLP ana l y sisR e ce i ve d:J a nua ry 11,2010 Accep t e d:Fe b rua ry 23,2010Suppo rted by Na ti o nal 863Prog ram i n t h e 11th Five 2ye a r P l an ni n g Pe ri o d (2006AA1001082322);Na ti ona l Na tural Science Fo unda ti o n (36;3);S T y j f x O ff f (G 5);Y G F f x N U y (3)32jf @j A s one of vege tab l e c rop s with the l a rge s t cu lti va ti on a re ai n C hi na ,p epp e r (Ca ps i cum a nnuu m L .)is a wo rl d w ide ki nd of vege ta bl e s and p roc e ssing condi m e nts .Acco rdi ng t o the sta tis ti c s,a nnua l c ultiva ti o n a rea of peppe rs i n C hi na ha s re ac he d abo ve 1400tho usa nd h m 2,whi ch m a ke s gre a t con 2tri buti o n to annua l a ve ra ge ba lance supply of vege ta bl e s inChina [1].W ith obvi o us adva nta ge s in p re 2m a turity,hi gh yi e ld a nd fru itw e i ght,peppe r hybri d s a re pop ul a r w it h m any fa r m 2e rs .It is expe nsive t o bree d hyb ri d see ds by a rti fic i a l em a scula ti on a nd po lli na ti on .I t is l a bou r 2sa vi ng t o ob t a i n hy 2bri d se ed s F 1by cytop l a sm i c m a le ste ril e li ne of peppe rs,which ca n e nsure the p urity o f se e ds and p ro t e c t the ri ghts of bree de rs,a nd is a lso the m o st e ffi c ient wa y t o re duce se e dco st a t p re se nt [2]o r the m a j o r direc ti o n fo r curre nt p epp e r bree ding .Thus,be side s e nha ncem en t o f the i de nti f i ca ti o n a nd b re ed i ng of c yt opl a s m i c m a l e ste ril e li ne o f peppe rs,stud 2i e s shoul d be ca rried out a t di ffe ren t l eve ls from b i o l ogy,which w ill p r o vi de a theo re tica l ba sis f o r furthe r effi c i e nt o r w i de u tili za ti on .R ul e s of inhe ritance in m o r p hol ogy,cyt o l ogy,POD isozy m e a nd m a l e 2ste ril e cha ra c t e rs ha ve be en a lrea dy dis 2cu ssed t ho roughl y i n studi e s o n t he m e cha nis m of cyt op l a sm i cm a le ste ril e li ne of peppe rs [3-4].Howe ve r,the re is little report on the m o l e cul a r m e chan is m of ste rility i n pe ppe rs,e spe c i a ll y se que nce fra g m e nts re la ted to cyt op l a s m ic m a l e s te ril ity of peppe rs .The re fo re ,ge nom ic DNA fr om c ytop l a s m i c m a le ste ril e l ine 21A a nd its m a i n ta i ne r l ine 21B o f peppe rwa s com 2pa ra ti ve l y ana lyze d by AFLP t o ob ta i n t he spe cifi c am p l ifi e d fragm en ts re l a ted to CM S i n this s tudy,wh i ch l a id a fo unda 2ti on fo r fu rthe r studi e s on mo l e cula r m e cha nis m of the cyt o 2pla sm ic m a l e ste rilit y in p epp e rs,a nd pro vide d a refe re nce fo r be tte r utili za ti o n of cyt op l a s m ic m a le ste rility i n peppe rs .M a te ria ls a n d M e th o d sMa te ria l sThe cyt op l a s m ic m a l e ste ril e line 21A w ith comp l e t e l y s ta 2ble ste rility tra its a nd its m a inta i ne r l ine 21B of peppe rs we re from Ve ge table Re se a rch I ns titute ,J i a ng su Aca dem y of Agri 2c ult u ra l Sc i e nce s .S ee dli ngs of 21A a nd 21B we re bred on the coo l ing bed i n e a rl y J a nua ry,w hil e i n l a te Ma rch,100p l a nts of 21A a nd 21B we re re spe c tive l y p l a nte d with conve nti ona l fie l d m a na gem e nt i n p l a s ti c gre e nho use s of Ve ge tab l e R e 2sea rch I ns titute ,J i a ng su Aca dem y of Ag ri cultura l S c i e nc e s .Me thod sA FL P R e fe rence d by Vo s [5]a nd LOU Q un 2feng [6],re stri c 2ti on fragm e nts a nd do uble 2stra nd DNA we re j o i nte d t oge the r w ith T 4li ga se through EcoR I and M se I doubl e di ge sti o n,w hil e re stri c ti o n e ndo nuc l e a se a nd T 4l iga se w e re a ll p urc ha se d from S ha ngha i S angon B i o l ogi c a l Engi ne e ri ng Te chno l o gy &S e rvi ce s Co .,Ltd .5μl d i ge sti o n p roduc t wa s used a s the tem p l a te f o r p re 2am p li fi ca ti on,w hil e the p ri m e rs of p re 2am p li fi 2c a ti on we re E +1a nd M +1.5μl p roduct of p re 2am p l ifi ca ti on wa s a na l yzed by 1%aga ro se ge l e l e c tropho re sis to e sti m a te the e ffi c iency of p re 2amp li fi c a ti on .Acco rding t o the te sti ng fo r p r o duc ts o f p re 2am p li fi c a ti on,the pro duc ts w e re dil ute d 10-30fo l d,a nd t he n u se d a s the tem pla te fo r se l e cti ve amp li fi c a 2ti on .F i na ll y,p roducts o f se lec tive amp li fica ti o n w e re sepa ra 2ted by 6%dena tured po l ya c ryl am i de ge l e lec tr opho re sis a nd dye d by s i lve r sta i ni ng .Re c yc ling o r c lon ing a nd se que nc e a na lys is of sp ec i f ic b and s D i ffe ren ti a l ba nds of cyt op l a sm i c m a le ste ril e li ne a nd its m a i nta ine r li ne we re re co ve re d a nd cut off a ccura te l y by the z f μ22S f 5f f 5μ2f 2Ag ri cu lt u ra l Science &Techno l o gy,2010,11(2):69-71C op yri gh t κ2010,I n f o r m a ti on Ins titute of HAAS.A l l right s re s erved.Agricultura lB i o techno l o gy0801200900781cience and e ch no l o g P ro ec t rom J i a ng i P ro vi nc i a l i ce o Educa ti on J J 0819o un g r ow thu nd o J i ang i o r m o l n i ve rs it 298.C o rre spo n di n g au tho r .E m ail :chen n au.e ste rili e d sca l pe l t o p ut i nto c en tri uga l tube s w ith 200l re d is till e d w a t e r .am p l e s w e re p l a ce d i n t o bo ili ng w a te r o r 1m i n a nd then cen tri uge d o r 10m i n w ith 12000rpm.l supe rna tan tw a s use d a s the temp l a t e o r com bina ti on t o the correspo nd i ng pri m e rs aga i n w it h the sam e am plifi ca ti o n .Afte r the m olec ul a r we i ght o f amp li fied p r o duc ts wa s ve ri fi e d,and t he n li ga ted t o pGE M Tea sy ve ctor .Com pe te nt E .co li JM 109wa s transfe rre d by the li ga ted p roduc ts fo r b l ue 2wh i te se l e c ti on.S ing l e 2co l ony p l a sm i d wa s e xtrac te d f o r e ndo nuc l e a se re a c 2ti on,and the n se nt to S ha ngha i I nvi troge n B i ote chno l o gy Co .,Ltd fo r DNA se que nc ing .The who l e sequence of j o i nts we re rem oved a fte r c l oning a nd se que nc i ng,a nd then subm i tted t o NCB I da ta ba se fo r BLAS T .R e s u lts a n d A n a lys isA FL P sp ec if i c f ra gm e n t s c ree n ing of g enom e from cyto 2p lasm ic m a le s te rile line 21A a nd its m a in ta in e r li n e 21BGenom ic DNA from cyt op l a sm ic m a l e ste ril e li ne 21A a nd its m a i nta ine r line 21B w e re am plifi e d w it h 32pa i rs o f AFLP pri m e r com bi na ti ons,a nd the re sults showe d tha t bands in AFLP m ap we re ri c h w ith 60bands i n ea ch l a ne.O nl y amp li 2fi e d p roduc ts from o ne pa i r of pri m e r had di ffe rence be t w e e n cyt op l a s m i c m a l e s te ri le li ne and its m a inta i ne r li ne i n 32p a irs of AFLP p ri m e rs,wh i ch indica te d tha t the re wa s l ittl e diffe r 2e nc e in ge nom i c DNA be t we en c yt op l a s m i c m a l e ste ril e li ne 21A a nd its m a i n ta i ne r li ne 21B.Genom ic DNA from cyt op l a sm ic m a l e ste ril e li ne 21A a nd its m a inta i ne r l ine 21B we re am p li fi e d a ga i n w ith p ri m e r com bi 2na ti on EcoR 2AA /M se I 2CAG scree ned by poly mo rphic pri m e r pa irs .The re wa s one spe c i fi c band w ith 200bp in cyt op l a sm i c m a le ste ril e li ne 21A (F ig .1),w hil e no ba nd wa s f o und i n its m a inta i ne r li ne 21B.Aft e r the repe a te d te st,the fra g m e ntswe re demo nstra te d to be sta bl e ,re li a bl e and a sp ec i fic fra g 2m e nt re la ted t o m a l e 2ste rile c ha rac ters.A:Am p li fi cati o n p rodu cts o f p ri m e r EAA /M C AG,and the arr ow show s the d i ffe ren ti a l fragm e nts;B :S i ng l e c l o ne i d entifi ca ti on o f d i ffere ntial fragm e nts .F i g.1 Am p l i fi ca ti o n of AFLP d i ffe ren ti a l fragm e nts o f ge nom i cDNA from s t e ril e l ine 21A and its m ai n tai n er l ine 21BRe c yc ling o r c lon ing a nd se qu enc i n g of A FL P d i ffe re n ti a l f ra gm e ntsF rom F i g .1-B ,re com bi nan tp la s m id PCR p roduc ts we re i den ti c a lw ith the si z e of ta rge t fra g m ents,which show ed tha t ta rge t fra g m e nts ha d a lre ady be en succ e ssfu l ly T 2A cl one d .Afte r ve cto r se que nce a nd AFLP p ri m e r com bi na ti o n AA /CAG sequence we re rem ove d,the se que nc i ng sequence w a s 169bp i n ful l length (F i g .2).Thu s,the spe c i fi c f ragm e nt i n c ytop la s m i c m a l e ste ril e li ne 21A wa s m a rked a s AA /CAG 169t o ana l yze co nvenientl y.The und erli ned pa rt s are AA /CAG se quence s of AFLP p ri m e r com b i na ti o n ,wh il e the do uble 2u nde rli ned pa rts are AF LP j o i nt s .F i g.2 Sp eci fi c fragm en t sequ ences o f s teri le li ne 21ABLA S Tn a na lys is of AA /CAG 169s equ en ce in sp ec ific f ra g 2m en tsAcco rdi ng t o BL AS T re sults,pa rti a l sequence s of AA /CAG 169(l oc a ted in 122-158bp ,AGA ATTGGT A C G 2CAGTC T AT G ATG AGT CC T G AGT A AC ,w ith 37bp i n tota l)had a h i ghe r si m il a ri ty t o seve ra l sequence s o f t he re l a te d AFLP m a rke r se que nc e s with the sex and grow th tra its in P ena eu s mo nodon (AFE549M 27.1).Furthe r ana l ys is re 2ve a led tha t 37bp se que nce s we re four se ri e s re pea ted se 2que nce s of t he re l a te d AFLP m a rke r se que nc e s w it h the se x a nd grow th tra its in Pe nae us m ono don (F i g .3),so the spe 2c i fi c fra g m ent AA /CAG 169w a s cl o se l y re l a te d t o its ste rilit y .BLA S TX a na lys is of AA /CAG 169s equ en ce in sp ec ific f ra g 2m en tsA A /CAG 169se que nc e wa s subm itt e d to GeneB a nk,a nd a na l yze d by BLASTX o f NCB I a nd p rote in da ta ba se s fo r ho 2mo l ogy sea rching .The re sults sugge sted tha t am ino a c i d se 2que nce deduce d by this fra g m ent had a hi ghe r s i m ila rit y t o pa rtia l se que nce s of So l a nu m dem i s sum p uta tive ga g 2po lp ro 2t e i n a nd So l a num dem issu m puta ti ve re tr o tra nsposon p r o te in re sp ec ti ve ly (F i g .4).T BL AS TX a na l ysis i ndica te d tha t am i no a c i d se que nce deduce d by this fragm en t ha d a lso a hi ghe r y q f S 2,S S ,2F i g.3 A li gnm en t of t he pa rti al seque nce s of AA /C AG 169andthe re l a t e d AFLP m arke r se quence s with the s ex andgrow th t raits i n Penaeu s m o no done nco de d by gag (g roup 2sp ec ifi c a ntige n )ge ne i n the struc 2tura l ce n t e r of p lan t re tr o tra nspo so n,a nd the n be cam e se v 2e ra l kind s of l e ukocyte p ro te i n s a fte r pos t 2tre a t m e nt .Gag 2po l p r o te i n wa s e ncoded by po l 2ga g i n com m on,a nd the n be 2,f 2z y T f ,f f f f 2G 6y y 07Ag ri cultura l Sc i ence &Tech no l o gy Vo l .11,No.2,2010si m il a rit t o pa rti a l se uence s o o l anum dem is sum pu t a ti ve gag po l p rote i n o lanu m dem issum puta ti ve re tro tra nspo so n pro te i n a nd o l a num dem issu m puta ti ve re tr o tra nsposon ga g pro te i n .Howe ve r gag p ro te i n wa s a m ulti p r o te in p re curso rc am e re ve rse tra nsc ri p t a se inte gra se a nd pro te a se a te r en m e di ge sti on.he re ore the unc ti o n o this spe c i ic ra ge m e n t AA /CA 19wa s m o re like l to be c l o se l re la te d t o re tro tra nspo son .F ig.4 B LASTX a nalys i s o f t he w ho le sequ ence o f C M S 21A 169D is c u s s io n sM e chanism of m a l e s te ril ity ge ne s ha s bee n w i de l y stud 2i ed a t hom e a nd a broa d,a nd it is ge ne ra lly conside red tha t the o ccu rrence of plant CMS i s c l ose ly re l a te d t o the struc ture a nd exp re s si o n of m i tocho ndria l ge nom e ,whil e the frequent re com bi na ti on o f intra 2m o l e cul a r a nd i nte r 2mo l e cula r m i tochon 2dri a l genom e is m o l e cul a r ba sis f o r the occu rre nce o f C M S.F rom a l a rge amo un t of m it o chondri a l DNA fragm e nts i n the i denti fi e d CM S ,the y a re a ll gene ra ll y ch i m e ri c ge ne s from pa rtia l sequence s o f se ve ra l i den ti fi e d ge ne s afte r m ulti 2re 2com bi na ti on a nd the un i de nti fi e d re a di ng fram e (urf),such a ska l e nap us [7],m a ize [8]a nd ri ce [9].The t o ta l ge nom i c DNA e xtrac te d by C T AB i s cons titute d by nuc lea r DNA,m t D NA a nd cpDNA,so the spec ifi c fra g m ent AA /CAG 169of ste ril e li ne 21A po ssibl y com e s fr om nuc l e a r DNA,and is a lso p rop e rl y from m t DNA o r cpDNA.The ope n re ad i ng fram e of the sp ec i fic fra g m e nt A A /CAG 169se que nce is found w ith the on l ine t oo l of O RF F i nde r in NCB I .Com bi ne d w it h BLAS T X and T BLASTX a na l ysis,the op en rea di ng fram e of the sp ec i fic fra g m e nt AA /CAG 169se que nce is pri m a ril y i denti fi e d to be -1encodi ng f ram e fo r 53am i no a c i ds.W he re a s,the true i de nti f i ca ti o n of t h i s re a di ng fram e is still unknown,a nd its re l a ti onshi p w i th ste ril e chi m e ri c ge ne s nee ds furthe r studi e s .BL AS Tn in this study re ve a ls tha t the spe c i fi c fra g m e nt AA /CAG 169is li ke l y t o be c l o se l y re l a te d t o ste rili ty .Howe ve r,BLAS T X and T BL AS T X a ll show tha t AA /CAG 169sequence is po ssibl y re l a te d t o re tro tra nspo son,which i ndica te s tha t c yt op l a s m i c m a l e ste rility o f peppe rs m a y be c l o se l y re l a te d t o re tr o tra nspo son .The si m il a r re sults a rea lso repo rted i n cyt op l a s m ic m a le ste rilit y of ca rro ts [10],whi c hl a ys a founda ti on fo r furthe r s t udie s on m o l ec ul a r m e cha nis mof cyt opla s m ic m a l e ste rilit y in peppe rs .R e fe re n c e s[1]F ANG R (方荣),CHEN XJ (陈学军),M I A O NS (缪南生),et a l.Adva nce s i n gene ti c r e sou rces and mo l ecular breed i ng o f pepp er (Cap s i cum spp.)(辣椒(Cap s i cum spp.)遗传资源与分子育种进展)[J ].Acta Ag ri cult u rae J i angxi (江西农业学报),2004,16(3):55-61.[2]WANG SB (王述彬),ZHAO HL (赵华仑),L I U J B (刘金兵),et a l.He t e r o s is util i za ti o n o f C MS li ne s i n ho t (swee t )p eppe rs and t e ch 2ni q ues fo r i ts hybri d seed p roducti o n (辣(甜)椒胞质雄性不育系杂种优势利用及其制种技术)[J ].J i ang su J ou rnal o f Ag ri cultura l Sci 2ences (江苏农业学报),2002,18(3):143-146.[3]DA ILF (戴亮芳),LUO XD (罗向东),WA N G S B (王述彬),et a l.I so zym e s anal y ses o f cyt op l a s m i c m a l e s teri l e (C M S )l i ne i n pep 2p er (C ap s i cum annuum L.)(辣椒细胞质雄性不育系的3种同工酶分析)[J ].Ac t a Bo tan i ca B o reali -Occi den t a li a Si nica (西北植物学报),2007,27(9):1772-1776.[4]LUO X D ,DAI LF ,WANG SB ,et a l .Ma l e g am ete deve l o pm entand ea rl y t ape t a l degene rati on in cyt op l a s m i c m a l e ste ril e pepp er (Cap s i cum annuum L.)i n ve st i g ated by m e i o ti c,ana t o m i ca l and ultras tructu r a l ana l yse s[J ].Pl an t B reed i ng,2006,125:395-399.[5]V O S P,H O GER R ,BLEEKER M.AF LP:a n ew techni qu e f o rDNA fi ngerp ri n ti ng [J ].Nucl e i c Ac i ds R es,1995,23:4407-4414.[6]LOU QF (娄群峰),CHEN J F (陈劲枫),JAHN M,et al .I den ti fica 2ti o n of AF LP and SC AR mo l ecu l ar m a rke rs li nked t o gyno eci ou s l o 2ci in Cucum i s s ati vus L.(黄瓜全雌性基因连锁的AF LP 和SCAR 分子标记)[J ].Acta Ho rti cult u rae Si n i ca (园艺学报),2005,32(2):256-261.[7]Y ANG J H,ZH AN G M F,Y U JQ.M it ochond ri a l n ad2gene is co 2tran scri p ted w i th CMS 2as soci a ted o rf B ge ne i n cyt op l a s m i c m al e 2s teri l e s tem m us t a rd (B ra s si ca j uncea)[J ].Mo l ecu l a rB i o l o gy Re 2po rts,2009,36:345-351.[8]GALLAGHER LJ ,B ETZ SK,CHASE CD.M it ochond ri a l R NA edi 2ti n g trunca t e s a ch i m eri c op en reading fram e as sociated w i th S m ale 2s t e ril it y i n m a i ze[J ].C urr Gene t,2002,42:179-184.[9]ZHANG H,L I SQ ,YIP,e t al .A Hon gli an C MS li ne of rice dis p l aysabe rran t F 0of F 0F 12ATPas e [J ].P l ant C e l l R epo rts,2007,26:1065-1071.[10]NAK AJ I MA Y,Y A MA MO TO T,MUR ANAK A T,et a l .Gene ti c var 2i ati o n o f p etal o i d m al e 2ste ri l e cyt op l a s m o f ca rro ts reveal ed by se 2que nce 2t agged s i tes (STS s )[J ].Theo r App l Gene t ,1999,99:837-843.R es p o n s i b le ed ito r:C H EN Xiu 2c h en R es p o n s i b le t ra n s la to r:L I J in g 2w e i R es p o n s i b le p ro o fread er:W U Xiao 2y an辣椒细胞质雄性不育系及其保持系的AFL P 分析(摘要)罗向东1,2,戴亮芳1,2,陈劲枫23,王述彬3 (1.南京农业大学园艺学院,江苏南京210095;2.江西师范大学生命科学学院,江西南昌330022;3.江苏省农业科学院蔬菜研究所,江苏南京210014)[目的]对辣椒细胞质雄性不育系及其保持系进行AFLP 分析,为进一步深入研究辣椒细胞质雄性不育的分子机制奠定基础。
保护麦夸里岛英语作文

保护麦夸里岛英语作文英文回答:Macquarie Island, a treasure among the vast expanse of the Southern Ocean, is a place of unparalleled beauty and natural wonder. Here, amongst the rugged cliffs and pristine waters, the call of the wandering albatross fills the air, echoing through time and space. It is a true testament to the power of nature, a living reminder of the urgent need for conservation.Every year, thousands of visitors flock to Macquarie Island, drawn by the magnetic pull of its pristine beaches and the fascinating wildlife that calls the island home. The island is a sanctuary for a variety of species, including the royal penguin, the southern elephant seal, and the endemic fur seal. However, with the charm and allure of this unique place, comes the responsibility to protect and preserve it.Tourism has significantly increased over the last decades, bringing with it many benefits, but also challenges. The delicate balance of the island's ecosystem is always at risk from human interference. As the number of visitors grows, so does the toll on the environment. It is our duty, as both residents and visitors, to be mindful of our actions and the potential impact on this fragile paradise.To safeguard this natural haven, stringent measures must be put in place. We must advocate for sustainable tourism practices, ensuring that the environmentalfootprint is minimal. This includes implementing eco-friendly policies, conducting impact assessments, and educating tourists on how to minimize their impact. It is only through collective effort that we can preserve the island's rich biodiversity.Furthermore, climate change poses a significant threat to Macquarie Island and its inhabitants. Rising sea levels and unpredictable weather patterns can have catastrophiceffects on the ecosystem. It is imperative that we address the root causes of climate change, working globally to reduce emissions and protect our planet for future generations.Our connection to Macquarie Island goes beyond mere fascination; it is a deep-seated bond, an intrinsic part of our humanity. It is the call of the wild, the longing for untouched corners of nature, the shared responsibility to ensure that places like Macquarie Island remain pristinefor years to come.In conclusion, the preservation of Macquarie Island is a call to action—a call for unity, awareness, and action. By taking proactive steps to protect this natural gem, we are not only safeguarding wildlife and the environment but also preserving our own humanity. Macquarie Island stands as a beacon of hope, a reminder that even in a world increasingly touched by human hands, there are still untouched places that resonate with purity and life. May we all heed this call, and in doing so, leave a lasting legacyfor generations to come.中文回答:麦夸里岛,是南冰洋广袤海域中的一颗珍宝,这里拥有无与伦比的美丽和自然奇观。
- 1、下载文档前请自行甄别文档内容的完整性,平台不提供额外的编辑、内容补充、找答案等附加服务。
- 2、"仅部分预览"的文档,不可在线预览部分如存在完整性等问题,可反馈申请退款(可完整预览的文档不适用该条件!)。
- 3、如文档侵犯您的权益,请联系客服反馈,我们会尽快为您处理(人工客服工作时间:9:00-18:30)。
II. THEORETICAL FRAMEWORK
The standard starting point is the effective ∆B = 1 weak Hamiltonian GF ∗ u u ∗ c c Vub c1 (µ)O1 (µ ) + c2 (µ )O 2 (µ) + Vcq Vcb c1 (µ)O1 (µ ) + c2 (µ )O 2 (µ ) Heff = √ Vuq 2
data than using Bauer-Stech-Wirbel (BSW) form factors [12]. We find further that some V V modes and the K ∗ η modes are promising. Processes that are basically pure T (e.g. ρ+ ρ0 ) or pure P (e.g. K (∗) φ) depend only weakly on γ , and thus offer direct tests of factorization. If large CP asymmetries (aCP ) are observed in the K (∗) φ modes, it could be a signal for new physics. The paper is organized as follows. In Sec. II a brief review of the theoretical framework is given. We then sketch how sensitivity to γ angle emerges. In Sec. III we discuss in detail the hints of negative cosγ from existing data. We show that the form factors from LC sum rules are preferred by data. Adopting LC sum rule form factors, in Sec. IV we study the V V modes as well as some other modes that can offer further tests for cos γ < 0 or the factorization hypothesis. Finally, the discussion and conclusion are presented in Sec. V.
Kwei-Chou Yang
of Physics, National Taiwan University, Taipei, Taiwan 10764, R.O.C. of Physics, Academia Sinica, Taipei, Taiwan 11529, R.O.C.
2 Institute 3 Department
Typeset using REVTEX 1
I. INTRODUCTION
Experimentally, a number of hadronic rare B decay modes have been observed [1–3] in the last two years. They may allow us access [4–7] to unitarity angles of the Kobayashi∗ Maskawa (KM) matrix such as γ (≡ arg(Vub ) in standard phase convention), by exploiting
O8(10) =
3 2
′ ′ qβ qα )V +A(V −A) (¯ qα bβ )V −A , eq′ (¯ q′
with O3−6 , O7−10 the QCD, electroweak penguin operators and (¯ q1 q2 )V ±A ≡ q ¯1 γµ (1 ± γ5 )q2 . The decay amplitude is computed by evaluating the hadronic matrix elements of Heff , i.e. ci (µ) Oi(µ) = ci (µ)gij (µ) Oj
∗ ). We update if, contrary to current fits, one has cos γ < 0 where γ ≡ arg(Vub
previous results with light cone sum rule form factors, which seem to work better. We then discuss various B → V V modes as well as the K ∗ η modes. Finding the pattern of ρ+ ω 0 < ρ+ ρ0 , K ∗+ ρ−,0 > K ∗0 ρ+ , K ∗+ ω 0 > K ∗0 ω 0 and K ∗+ η > K ∗0 η would strengthen the support for cos γ < 0. The electroweak penguin enhances (suppresses) the K ∗+ ρ0 (K ∗0 ρ0 ) rate by a factor of 2, and finding K ∗+ ρ0 ≃ K ∗+ ρ− would be strong evidence for the electroweak penguin. PACS numbers:
O3(5) =
q′
(¯ q ′ q ′ )V −A(V +A) (¯ qb)V −A , 3 2 q ′ q ′ )V +A(V −A) (¯ q b)V −A , eq′ (¯
q′
O4(6) =
q′
′ ′ (¯ qβ qα )V −A(V +A) (¯ qα bβ )V −A ,
(2.2)
O7(9) =
Tree-Penguin Interference and Tests for cos γ < 0 in Rare B → P P , P V and V V Decays
arXiv:hep-ph/9908202v1 2 Aug 1999
1 1 Department
Wei-Shu Hou and
2,3
interference between tree and penguin amplitudes in these modes. The presently observed decay processes can be catalogued into two classes. The first class, e.g. B → ρπ , is dominated by tree (T) level b → u transitions, but may have sizable penguin (P) contributions. The second class, e.g. B → Kη ′ , Kπ, and the newly observed K ∗+ π − mode, are penguin dominant processes which may have sizable T/P. Two-body decays of B mesons are usually studied under the factorization hypothesis. Based on this hypothesis, the decay amplitude is given in terms of a weak transition amplitude and the decay constant of a factorized final state meson. Nonfactorizable contributions are lumped into the effective number of colors Neff which may deviate from Nc = 3. The current fits of KM parameters give γ in the range of 60◦ − 70◦ [8], which heavily relies on the lower limit ∆mBs > 12.4 ps−1 from combining LEP, CDF and SLD data. With a little loosened limit ∆mBs > 10.2 ps−1 [9] at 95% C.L., some room is allowed for negative cos γ . If one adopts, however, the currently favored γ ≃ 60◦ − 70◦ , it is difficult to explain present data such as K + π − ∼ K 0 π + ∼ K + π 0 ∼ 1.5 × 10−5 , π + π − < 0.84 × 10−5 , and the strength of the newly observed ρ0 π + ∼ 1.5 × 10−5 and K ∗+ π − ∼ 2.2 × 10−5 [3]. All the data so far therefore seem to prefer cos γ < 0 if factorization holds [7], except the size of K + ω 0 ∼ 1.5 × 10−5 [2] which cannot be explained by factorization [See Note Added.]. However, all modes with branching ratios (Br) of order 10−5 or more will likely be updated or measured soon by CLEO and the B factories. It is thus of interest to explore any additional modes that can shed further light on γ . In this paper we extend Ref. [7] and study additional channels [10] for which the γ range can be probed. We update the B → P P and P V modes (P , V stand for pseudoscalar and vector mesons) with form factors from light-cone (LC) sum rules [11], which seem to give a better fit to 2