Borg-Marchenko-type Uniqueness Results for CMV Operators

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蛋白质二级结构预测软件

蛋白质二级结构预测软件

通过EMAIL进行序列检索 当网络不是很畅通时或并不急于得到较多数量的蛋白质序列时, 可采用EMAIL方式进行序列检索。 蛋白质基本性质分析 蛋白质序列的基本性质分析是蛋白质序列分析的基本方面,一 般包括蛋白质的氨基酸组成,分子质量,等电点,亲水性,和 疏水性、信号肽,跨膜区及结构功能域的分析等到。蛋白质的 很多功能特征可直接由分析其序列而获得。例如,疏水性图谱 可通知来预测跨膜螺旋。同时,也有很多短片段被细胞用来将 目的蛋白质向特定细胞器进行转移的靶标(其中最典型的例子 是在羧基端含有KDEL序列特征的蛋白质将被引向内质网。 WEB中有很多此类资源用于帮助预测蛋白质的功能。
特殊结构或结构预测 COILS http://ulrec3.unil.ch/software/COILS_ form.html MacStripe /matsudaira/m acstripe.html
与核酸序列一样,蛋白质序列的检索往往是进行相 关分析的第一步,由于数据库和网络技校术的发展, 蛋白序列的检索是十分方便,将蛋白质序列数据库 下载到本地检索和通过国际互联网进行检索均是可 行的。 由NCBI检索蛋白质序列 可联网到: “:80/entrz/qu ery.fcgi?db=protein”进行检索。 利用SRS系统从EMBL检索蛋白质序列 联网到:/”,可利用EMBL 的SRS系统进行蛋白质序列的检索。
跨膜区域 TMpred: /software/TMPRED_form.ht ml 预测蛋白质的跨膜区段和在膜上的取向,它根据来自SWISSPROT的跨膜蛋白数据库Tmbase,利用跨膜结构区段的数量、 位置以及侧翼信息,通过加权打分进行预测。Tmpred的Web 界面十分简明。用户将单字符序列输入查询序列文本框,并可 以指定预测时采用的跨膜螺旋疏水区的最小长度和最大长度。 输出结果包含四个部分:可能的跨膜螺旋区、相关性列表、建 议的跨膜拓扑模型以及代表相同结果的图。

GS_97034-2_Oberflaechenpruefung_von_Kfz-Innenraummaterialien_Fingernageltest_2015_09

GS_97034-2_Oberflaechenpruefung_von_Kfz-Innenraummaterialien_Fingernageltest_2015_09

BMW Group Standard GS97034--22015--09Deskriptoren:Abrieb,Fingernageltest,Innenraum,Material,Oberfläche Ersatz fürGS97034--2:2007--05Descriptors:Abrasion,finger nail test,interior,material,surface Replacement forGS97034--2:2007--05Oberflächenprüfung von Kfz-Innenraummaterialien FingernageltestSurface test of motor vehicle interior materialsFinger nail testAusdrucke unterliegen nicht demÄnderungsdienst.Print-outs are not subject to the change service.Fortsetzung Seite2bis5Continued on pages2to5BMW AG Normung:80788MünchenE BMW AG interleaf-doc Alle Rechte vorbehalten/All rights reserved Bearbeiter/Editor:Norbert MaierSeite/Page2GS97034--2:2015--09In case of dispute the German wording shall be valid. Vorwort ForewordDieser Group Standard wurde mit den verantwortlichen Bereichen der BMW Group abgestimmt.This Group Standard has been coordinated with the responsible departments of the BMW Group.Für die in der Norm zitierten nationalen Normen wird in der folgenden Tabelle auf die entsprechenden internationalen Normen hingewiesen:For the national standards quoted in the subject standard,the following table refers to the corresponding international standards:Nationale Normen National standards Internationale Normen International standardsDIN EN ISO291ISO291Änderungen AmendmentsGegenüber GS97034--2:2007--05wurden folgendeÄnde-rungen vorgenommen:The following amendments have been made to GS97034--2:2007--05:--zitierte Normen aktualisiert;--cited standards updated;--Norm komplettüberarbeitet.--standard completely revised. Frühere Ausgaben Previous editions2007--051Anwendungsbereich und Zweck1Scope and purposeDieser Group Standard beschreibt den Fingernageltest mit einem Kunststoff-Prüfkörper,er gilt für lackierte,beschich-tete,unbeschichtete und kaschierte Oberflächenmaterialien.This Group Standard describes the finger nail test using a plastic test object.It applies to painted,coated,uncoated and laminated surface materials.Dieses Prüfverfahren dient zur Bestimmung der Wider-standsfähigkeit von Oberflächenmaterialien im KFZ-Innen-raum gegen durch Fingernägel verursachte Beschädigun-gen.This test procedure is used for determining the resistance of surface materials in the passenger compartment against damage caused by finger nails.Zweck dieses Group Standards ist die Festlegung einer ein-heitlichen Prüfung zur Eignung des Materials.The purpose of this Group Standards is the definition of a standardized test for material suitability.Die Festlegung der zu prüfenden Teile ist mit den betroffenen Fachstellen abzustimmen.The components to be tested shall be defined in agreement with the concerned specialist departments.Die für das Oberflächenmaterial gültigen Anforderungen wer-den in den entsprechenden Regeln z.B.Technische Liefer-vorschrift,Group Standard oder Lastenheft für Werkstoffe und Bauteile festgelegt.The requirements applicable to the surface materials are defined in the respective regulations,e.g.Technical Delivery Specifications,Group Standards or Requirements Specifications for materials and components.2Normative Verweisungen2Normative referencesDiese Norm enthält Festlegungen aus anderen Publikatio-nen.Diese normativen Verweisungen sind an den jeweiligen Stellen im Text zitiert und die Publikationen sind nachstehend aufgeführt.Es gilt die letzte Ausgabe der in Bezug genomme-nen Publikation.This Standard incorporates provisions from other publications.These normative references are cited at the appropriate places in the text and the publications are listed hereafter.The respective latest edition of the publication is applicable.AA--0546Universalkratzprüfstand(UKP)AA--0546Universal scratch testerAA--0635Bewertungsschema nach diversenOberflächenprüfungen von KFZ--In-nenraummaterialien AA--0635Evaluation scale after different surfacetests of motor vehicle interior materialsDIN EN ISO291Kunststoffe;Normalklimate für Kondi-tionierung und Prüfung DIN EN ISO291Plastics;Standard atmosphere forconditioning and testingSeite/Page3GS97034--2:2015--09 3Prüfung3Test3.1Prüfverfahren 3.1Test procedureVor der Prüfung sind die Proben24h bei Normalklima nach DIN EN ISO291--23/50(Klasse2)zu konditionieren.Prior to the test,the samples shall be conditioned for24hours under standard atmosphere according to DIN EN ISO291--23/50(class2).Die Proben werden bei Normalklima nach DIN EN ISO291--23/50(Klasse2)geprüft.The samples are tested under standard atmosphere according to DIN EN ISO291--23/50(class2).Der vorgegebene Prüfkörper wird relativ zur Prüffläche mit konstanter Prüfkraft und Geschwindigkeit bewegt.The specified test specimen is moved relatively to the test surface with a constant test pressure and at constant speed.Die Prüfkraft wird in Etappen gesteigert.Hierbei wird die zu prüfende Oberfläche für jede Prüfkraft einmalig belastet.The test force is increased in steps.Each test force is applied once only to the surface to be tested.Bevorzugt sind die Prüfungen an ebenen Proben durchzufüh-ren.The tests shall be preferably performed on flat samples. Der Prüfweg soll60mm bis100mm betragen.The test path should amount to60mm to100mm.Für die Durchführung der Prüfung ist grundsätzlich jeder Prüf-aufbau geeignet,welcher eine Prüfung im Rahmen der aufge-führten Parameter ermöglicht.Every test setup permitting a test within the scope of the listed parameters is suitable for test execution.Beispiele geeigneter Prüfaufbauten sind unter Abschnitt6ge-nannt.Examples of suitable test setups can be found in section6.3.2Prüfparameter 3.2Test parametersFür Folien,Häute,Leder,Kunstleder und dekorative Oberflä-chen:For foils,skins,leather,artificial leather and decorative surfaces:Prüfgeschwindigkeit:(200±20)mm/s Test speed:(200±20)mm/sPrüfkraft:5N,10N,15N,20N Test force:5N,10N,15N,20N Für Spritzgussoberflächen:For injection molded surfaces:Prüfgeschwindigkeit:(700±20)mm/s Test speed:(700±20)mm/sPrüfkraft:2N,4N,6N,8N,10N Test force:2N,4N,6N,8N,10NSeite/Page4GS97034--2:2015--093.3Prüfkörper 3.3Test specimenBezeichnung:Fingernageltester Kunststoff Designation:Finger nail tester plastic Werkstoff:PMMA Material:PMMAAbmessung:Durchmesser16mmDicke1mmRundungsradius vom Scheibenrand0,5mm Dimension:Diameter16mmThickness1mmRounding radius of disk rim0.5mmHärte:Shore D85Hardness:Shore D85Einbaulage:längs zur Prüfrichtung Installation orienta-tion:longitudinally to testing directionOberfläche:eine definierte Rauigkeit ist durchAbziehen mit Schleifpapier Körnung500zu erzielen Surface:a defined roughness shall beachieved by sanding using500-gritsandpaperDie Bezugsquelle kann bei der Fachstelle“Labortechnik Standort München”erfragt werden.The source can be requested from the specialist department “Laboratory TechnologyMunich”.Bild1Prüfrichtung Figure1Testingdirectionr=0,5mmBild2Prüfkörper Figure2Test specimenUm reproduzierbare Ergebnisse zu erhalten,darf pro Versuchnur einmal die gleiche Oberfläche des Prüfkörpers verwendetwerden.Um dies sicherzustellen ist der Prüfkörper nach je-dem Versuch um45°zu drehen.To achieve reproducible results,the same surface of the testspecimen shall be used only once per test.For this reason,thetest specimen shall be rotated after every test by45°.Sind alle8Flächensegmente verbraucht,wird die Prüfkörpe-roberfläche durch Abdrehen mittels Abstechmeißel und kur-zes Anschleifen mit Schleifpapier der Körnung500nachbear-beitet.Dies kann solange wiederholt werden bis der Durch-messer des Prüfkörpers15mm beträgt.Once all8area segments are used up,the test specimen isreconditioned via turning using a lathe tool and brief sandingusing500-grit sandpaper.This can be repeated,until the testspecimen diameter amounts to15mm.Seite/Page5GS97034--2:2015--09 3.4Probe 3.4SampleProbengeometrie:Sample geometry:Probenlänge:≥90mm Sample length:≥90mmProbenbreite:≥40mm Sample width:≥40mm3.5Allgemeines 3.5GeneralIn Schiedsfällen sind die Prüfungen an einem Gerät im Hause BMW durchzuführen.In the case of ambiguous results,the test shall be performed on equipment at BMW.Vor der Prüfung sind die Proben mit einem mit Laborspülmittel (Mucasol3%ig in VE-Wasser)benetzten Mikrofasertuch ohne Druck zu reinigen.Prior to the test,the samples shall be cleaned without applying pressure using a microfiber cloth wetted with laboratory rinsing agent(3%Mucasol in DI water).Die Probe ist im Originalaufbau auf den Probenträger aufzu-bringen.Abweichungen sind im Prüfbericht zu dokumentie-ren.The sample(original structure)shall be installed on the sample carrier.Deviations shall be documented in the test report.4Auswertung4EvaluationDie geprüften Proben sind mit einem mit VE-Wasser benetz-ten Mikrofasertuch ohne Druck zu reinigen.The tested samples shall be cleaned using a microfiber cloth wetted with DI water without applying pressure.Die Beurteilung erfolgt nach einer Erholungszeit von24h.Evaluation takes place after a recovery period of24h.Die Auswertung erfolgt gemäßBewertungsschema nach AA--0635.Evaluation follows the evaluation matrix according to AA--0635.5Angaben im Prüfbericht5Specifications in the test reportIm Prüfbericht sind unter Hinweis auf diese Norm die Art,die Bezeichnung und der Aufbau des geprüften Materials und der Prüfaufbau anzugeben.The test report shall indicate the type,designation and structure of the tested material and the test setup,including reference to this standard.Von dieser Norm abweichende Prüfparameter sind im Prüfbe-richt zu dokumentieren.Test parameters deviating from this standard shall be documented in the test report.6Beispiel geeigneter Prüfaufbauten6Example of suitable test setups 6.1ABREX-Prüfstand 6.1ABREX test rigAbriebprüfstand ABREX der Fa.Innowep mit Erweiterung ”Dynamisches Fingernageltestmodul Automotive”.Nähere Informationen zum Prüfstand können bei der Fachstelle “Labortechnik Standort München”eingeholt werden.Abrasion test bench ABREX by Innowep with”Dynamic Fingernail Test Module Automotive”extension.Further information to the test bench can be obtained from the specialist department“Laboratory Technology Munich”.6.2Universalkratzprüfstand 6.2Universal scratch test rigDer Universalkratzprüfstand ist eine Eigenentwicklung der Fa.BMW.Nähere Informationen zum Prüfstand können bei der Fachstelle“Labortechnik Standort München/Werkstoff-technik Nichtmetall”oder bei der Fa.Erichsen eingeholt wer-den.The universal scratch test bench is an in-house development by BMW.Further information to the test bench can be obtained from specialist department“Laboratory Technology Munich/Materials Technology Non Metal”or company Erichsen.Das Prüfgerät ist in der AA--0546beschrieben.The test device is described in AA--0546.。

代谢组学ppt课件

代谢组学ppt课件
簇类分析(Hierarchical Cluster Analysis ,HCA) 簇类的独立软模式分类法(Soft In- dependent Modeling of Class Analogy, SIMCA)、PLS-DA (PLSDiscriminant Analysis) 、人工神经网络 (Artificial Neural Network,ANN) 等
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2 代谢组学的概念
在新陈代谢的动态进程中,系统地研究代 谢产物的变化规律,揭示机体生命活动代 谢本质的科学。
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Metabonomics ---英 Jeremy K.Nicholson 20世纪
90年代
生命体系对病理生理刺激或遗传改造所产生的 动态,多指标代谢响应的定量测定.
Metabolomics ---Oliver Fiehn需要,可将 组织行甲醇除蛋白、庚烷除脂肪及冻干等处 理),加至质谱仪,经历汽化,离子化、加速分离 及检测分析后即可得出相应代谢产物或是代谢 组的图谱。图谱中每个峰值对应着相应的分子 量,结合进一步的检测分析可以部分鉴定出化 学成分以及半定量关系。
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(3)常用的其他一些分析技术
代谢产物的检测、分析与鉴定是代谢组学 技术的核心部分,最常用的方法是有两种
NMR 质谱(MS)
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(1)核磁共振技术
原理
核磁共振技术核磁共振是原子核的磁矩在恒定 磁场和高频磁场同时作用,且满足一定条件时 所发生的共振吸收现,是一种利用原子核在磁 场中的能量变化来获得关于核信息的技术. 生 命科学领域中常用的有三种
气相色谱(GC) 高效液相色谱仪(HPLC) 高效毛细管电泳(HPCE)
往往与NMR或MS技术联用,进一步增加其灵敏性。 敏感性及分辨率提高,“假阳性”率也就越大

D2-40

D2-40

D2-40D2-40: An Overview of a Unique Immunohistochemical MarkerIntroductionImmunohistochemistry (IHC) is a widely used technique in the field of pathology that allows for the visualization of specific target antigens within tissues. One such marker is D2-40, which has gained significant attention due to its unique properties and potential diagnostic implications. In this article, we will discuss the characteristics of D2-40, its applications, and its relevance in various pathological conditions.D2-40: A Brief BackgroundD2-40, also known as Podoplanin, is a protein encoded by the PDPN gene. Initially identified as a podocyte membrane antigen, it was shown to play a crucial role in the development of lymphatic vessels. D2-40 is characterized by its expression in endothelial cells of lymphatic vessels, certain subsets of macrophages, and some neoplastic cells of mesothelial and epithelial origin.Expression Patterns and LocalizationD2-40 has shown a consistent pattern of expression in lymphatic endothelial cells throughout various organs and tissues. By utilizing D2-40 immunohistochemistry, these lymphatic vessels can be easily identified and distinguished from blood vessels, which are negative for D2-40 expression. The use of D2-40 has proven to be particularly helpful in studying lymphatic metastasis and lymphatic invasion in various cancers, such as breast, lung, and colorectal carcinomas.Applications in Tumor Diagnosis and PrognosisOne of the most significant clinical applications of D2-40 is in tumor diagnosis and prognosis. Research has shown that D2-40 expression is associated with lymphatic vessel density and lymph node metastasis in several types of cancers. In breast cancer, for example, the presence of D2-40-positive tumor cells in the lymphatic vessels has been correlated with an increased risk of nodal metastasis. Similarly, in lung cancer, D2-40 expression has been associated with an unfavorable prognosis.D2-40 as a Diagnostic MarkerThe distinctive expression pattern of D2-40 has made it a valuable diagnostic marker, especially in tumors where lymphatic invasion is an important factor. In malignant mesothelioma, D2-40 has been shown to be highly sensitive and specific for differentiating it from reactive mesothelial hyperplasia. The absence of D2-40 staining in reactive mesothelial cells helps in ruling out the possibility of malignancy, making it a useful tool for accurate diagnosis.Distinctive Features of D2-40Apart from its diagnostic significance, D2-40 also possesses some unique features that make it an interesting marker to study. Research has shown that D2-40 is involved in the regulation of cell migration, invasion, and metastasis. It has been demonstrated that D2-40 interacts with the C-type lectin-like receptor 2 (CLEC-2), leading to platelet aggregation and lymphatic vessel remodeling. These findings suggest potential therapeutic implications for targeting D2-40 in metastatic disease.ConclusionD2-40, or Podoplanin, is a unique immunohistochemical marker with specific expression in lymphatic endothelial cells and some neoplastic cells. It has become an important diagnostic tool in various cancers, aiding in the identification of lymphatic invasion and predicting prognosis. Additionally, its distinct features and involvement in cellular processes make D2-40 an intriguing marker with potential therapeutic applications. Further research and studies are needed to fully explore the role of D2-40 in various pathological conditions and its potential as a therapeutic target.。

小鼠genotyping步骤

小鼠genotyping步骤

小鼠genotyping步骤小鼠基因分型是一种用于确定小鼠特定基因型的实验方法。

这种方法通过检测小鼠体内的特定基因的突变来确定基因型。

基因分型对于研究小鼠的遗传特性、基因功能以及治疗疾病的效果都非常重要。

在本文中,我们将详细介绍小鼠基因分型的步骤。

步骤一:DNA提取首先,需要从小鼠体内提取DNA。

可以使用不同的方法来提取DNA,比如裂解细胞膜并分离DNA。

常用的DNA提取试剂盒也可以用于此目的。

提取出的DNA可以通过测量吸光度来确定浓度和纯度。

步骤二:PCR反应接下来,使用聚合酶链反应(PCR)扩增特定基因的DNA片段。

PCR反应需要以下成分:模板DNA,引物对(包括特异性引物和控制引物),聚合酶和dNTPs。

反应条件(温度、时间)需要根据使用的引物和PCR仪的要求进行优化。

步骤三:凝胶电泳PCR反应后,将反应产物进行凝胶电泳以检测DNA片段的大小和纯度。

通常使用琼脂糖凝胶进行电泳分析。

在电泳前,需要将PCR产物与DNA标记物(如DNA分子量标记物)混合,并注入琼脂糖槽中。

然后,通过通电来分离DNA片段。

通过与标准DNA片段比较,可以确定PCR产物的大小。

步骤四:基因型分析通过对凝胶电泳结果进行显微镜观察并与标准DNA标记物比较,可以确定小鼠的基因型。

凝胶电泳可以显示不同基因型的特征带。

例如,如果小鼠是纯合子型(两个等位基因完全相同),则只会显示一个特定大小的带。

如果小鼠是杂合子型(两个等位基因不同),则会显示两个大小不同的带。

步骤五:验证和文献回顾在确定基因型后,应该验证结果的准确性。

可以选择一部分样本进行重复测试,以确认结果的一致性。

此外,还可以参考相关文献以确保基因型的准确性和合理性。

注意事项:1.PCR反应条件需要根据实验需要进行优化。

特异性引物和控制引物的设计非常重要,以确保PCR反应的特异性和敏感性。

2.在进行凝胶电泳之前,确保琼脂糖凝胶被充分固化,并根据需求进行样品槽的制作。

3.在进行凝胶电泳时,要小心不要损坏琼脂糖凝胶或让凝胶与电极接触。

常用酿酒酵母菌株基因型

常用酿酒酵母菌株基因型

常⽤酿酒酵母菌株基因型Commonly used strainsinformation include:used lab strainsidentity between common lab strainsS288CGenotype:MATαSUC2 gal2 mal mel flo1 flo8-1 hap1 ho bio1 bio6Notes: Strain used in the systematic sequencing project, the sequence stored in SGD. S288C does not form pseudohyphae. In addition, since it has a mutated copy of HAP1, it is not a good strain for mitochondrial studies. It has an allelic variant of MIP1 which increases petite frequency. S288C strains are gal2- and they do not use galactose anaerobically.The S288C genome was recently resequenced at the Sanger Institute.References:Mortimer and Johnston (1986) Genetics 113:35-43.BY4743Genotype:MAT a/αhis3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15 ura3Δ0/ura3Δ0Notes: Strain used in the systematic deletion project, generated from a cross between BY4741 and BY4742, which are derived from S288C. As S288c, these strains have an allelic variant of MIP1 which increases petite frequency. See Brachmann et al. reference for details.References:Brachmann et al. (1998) Yeast 14:115-32.FY4Genotype:MAT aNotes: Derived from S288C.References:Winston et al. (1995) Yeast 11:53-55.FY1679Genotype:MAT a/αura3-52/ura3-52 trp1Δ63/TRP1 leu2Δ1/LEU2 his3Δ200/HIS3 GAL2/GALNotes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.References:Winston et al. (1995) Yeast 11:53-55.AB972Genotype:MATα X2180-1B trp10 [rho 0]Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. AB972 is an ethidium bromide-induced rho- derivative of the strain X2180-1B-trp1.References:Olson MV et al. (1986) Proc. Natl. Acad. Sci. USA 83:7826-7830.A364AGenotype:MAT a ade1 ade2 ura1 his7 lys2 tyr1 gal1 SUC mal cup BIONotes: Used in the systematic sequencing project, the sequence stored in SGD.References:Hartwell (1967) J. Bacteriol. 93:1662-1670.XJ24-24aGenotype:MAT a ho HMa HMα ade6 arg4-17 trp1-1 tyr7-1 MAL2Notes: Derived from, but not isogenic to, S288CReferences:Strathern et al. (1979) Cell 18:309-319DC5Genotype:MAT a leu2-3,112 his3-11,15 can1-11Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.References:Broach et al. (1979) Gene 8:121-133X2180-1AGenotype:MAT a SUC2 mal mel gal2 CUP1Notes:S288c spontaneously diploidized to give rise to X2180. The haploid segregants X2180-1a and X2180-1b were obtained from sporulated X2180YNN216Genotype:MAT a/αura3-52/ura3-52 lys2-801amber/lys2-801amber ade2-101ochre/ade2-101ochreNotes: Congenic to S288C (see Sikorski and Hieter). Used to derive YSS and CY strains (see Sobel and Wolin). References:Sikorski RS and Hieter P (1989) Genetics 122:19-27.YPH499Genotype:MAT a ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1Notes: Contains nonrevertible (deletion) auxotrophic mutations that can be used for selection of vectors. Notethat trp1-Δ63, unlike trp1-Δ1, does not delete adjacent GAL3 UAS sequence and retains homology to TRP1 selectable marker.gal2-, does not use galactose anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.References:Sikorski RS and Hieter P (1989) Genetics 122:19-27.YPH500Genotype:MATαura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1Notes:MATα strain isogenic to YPH499 except at mating type locus. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C. References:Sikorski RS and Hieter P (1989) Genetics 122:19-27.YPH501Genotype:MAT a/MATαura3-52/ura3-52 lys2-801_amber/lys2-801_amber ade2-101_ochre/ade2-101_ochretrp1-Δ63/trp1-Δ63 his3-Δ200/his3-Δ200 leu2-Δ1/leu2-Δ1Notes:a/α diploid isogenic to YPH499 and YPH500. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.References:Sikorski RS and Hieter P (1989) Genetics 122:19-27.Sigma 1278BNotes: Used in pseudohyphal growth studies. Detailed notes about the sigma strains have been kindly provided by Cora Styles.Sigma1278B background contain a nonsense mutation in RIM15, a G-to-T transversion at position 1216 that converts a Gly codon to an opal stop codon. This rim15 mutation interacts epistatically with mutations in certain other genes to affect colony morphology.Annotation of the Sigma1278b genome and information about the systematic deletion collection can be found here. SK1 Genotype:MAT a/α HO gal2 cup S can1R BIONotes: Commonly used for studying sporulation or meiosis. Canavanine-resistant derivative.The SK1 genome was sequenced at the Sanger Institute.References:Kane SM and Roth J. (1974) Bacteriol. 118: 8-14CEN.PK (aka CEN.PK2)Genotype:MAT a/α ura3-52/ura3-52 trp1-289/trp1-289 leu2-3_112/leu2-3_112 his3 Δ1/his3 Δ1 MAL2-8C/MAL2-8CSUC2/SUC2Notes: CEN.PK possesses a mutation in CYR1 (A5627T corresponding to a K1876M substitution near the end of the catalytic domain in adenylate cyclase which eliminates glucose- and acidification-induced cAMP signalling and delaysReferences:van Dijken et al. (2000) Enzyme Microb Technol 26:706-714W303Genotype:MAT a/MATα {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15} [phi+]Notes: W303 also contains a bud4 mutation that causes haploids to bud with a mixture of axial and bipolar budding patterns. In addition, the original W303 strain contains the rad5-535 allele. As S288c, W303 has an allelic variantof MIP1 which increases petite frequency.The W303 genome was sequenced at the Sanger Institute.References: W303 constructed by Rodney Rothstein (see detailed notes from RR and Stephan Bartsch).bud4 info: Voth et al. (2005) Eukaryotic Cell, 4:1018-28.rad5-535 info: Fan et al. (1996) Genetics 142:749W303-1AGenotype:MAT a {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15}Notes: W303-1A possesses a ybp1-1 mutation (I7L, F328V, K343E, N571D) which abolishes Ybp1p function, increasing sensitivity to oxidative stress.References: W303 constructed by Rodney Rothstein (see detailed notes from RR and Stephan Bartsch).ybp1-1 info: Veal et al. (2003) J. Biol. Chem. 278:30896-904.W303-1BGenotype:MATα {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15}References: W303 constructed by Rodney Rothstein (see detailed notes from RR and Stephan Bartsch).W303-K6001Genotype:MAT a; {ade2-1, trp1-1, can1-100, leu2-3,112, his3-11,15, GAL, psi+, ho::HO::CDC6 (at HO), cdc6::hisG,ura3::URA3 GAL-ubiR-CDC6 (at URA3)}References: K6001 was developed by Bobola et al in Kim Nasmyth's lab (PMID: 8625408), and has become a common model in yeast aging research (PMID: 15489200). Its genome has been sequenced by Timmermann et al (PMID: 20729566) D273-10BGenotype:MATαmalNotes: Normal cytochrome content and respiration; low frequency of rho-. This strain and its auxotrophic derivatives were used in numerious laboratories for mitochondrial and related studies and for mutant screens. Good respirer that's relatively resistant to glucose repression.References:Sherman, F. (1963) Genetics 48:375-385.FL100Genotype:MAT aReferences:Lacroute, F. (1968) J. Bacteriol. 95:824-832.Sources: ATCC: 28383SEY6210/SEY6211Genotype:MAT a/MATαleu2-3,112/leu2-3,112 ura3-52/ura3-52 his3-Δ200/his3-Δ200 trp1-Δ901/trp1-Δ901ade2/ADE2 suc2-Δ9/suc2-Δ9 GAL/GAL LYS2/lys2-801Notes: SEY6210/SEY6211, also known as SEY6210.5, was constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers, good growth properties and good sporulation.References:Robinson et al. (1988) Mol Cell Biol 8(11):4936-48SEY6210Genotype:MATαleu2-3,112 ura3-52 his3-Δ200 trp1-Δ901 suc2-Δ9 lys2-801; GALNotes: SEY6210 is a MATalpha haploid constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers and good growth properties.References:Robinson et al. (1988) Mol Cell Biol 8(11):4936-48SEY6211Genotype:MAT a leu2-3,112 ura3-52 his3-Δ200 trp1-Δ901 ade2-101 suc2-Δ9; GALNotes: SEY6211 is a MATa haploid constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers and good growth properties.References:Robinson et al. (1988) Mol Cell Biol 8(11):4936-48JK9-3dThere are a, alpha and a/alpha diploids of JK9-3d with the following genotypes:Genotypes: JK9-3da MAT a leu2-3,112 ura3-52 rme1 trp1 his4JK9-3dα has the same genotype as JK9-3da with the exception of the MAT locusJK9-3da/α is homozygous for all markers except mating typeNotes: JK9-3d was constructed by Jeanette Kunz while in Mike Hall's lab. She made the original strain while Joe Heitman isolated isogenic strains of opposite mating type and derived the a/alpha isogenic diploid by mating type switching. It has in its background S288c, a strain from the Oshima lab, and a strain from the Herskowitz lab. It was chosen because of its robust growth and sporulation, as well as good growth on galactose (GAL+) (so that genes under control of the galactose promoter could be induced). It may also have a SUP mutation that allows translation through premature STOP codons and therefore produces functional alleles with many point mutations.88(5):1948-52RM11-1aGenotype:MAT a leu2Δ ura3Δ ho::KanNotes: RM11-1a is a haploid derivative of Bb32(3), a natural isolate collected by Robert Mortimer from a California vineyard, as in Mortimer et al., 1994. It has high spore viability (80–90%) and has been extensively characterized phenotypically under a wide range of conditions. It has a significantly longer life span than typical lab yeast strains and accumulates age-associated abnormalities at a lower rate. It displays approximately 0.5–1% sequence divergence relative to S288c. More information is available at the Broad Institute website.References:Brem et al. (2002) Science 296(5568):752-5Y55Genotype:MAT a /MAT alpha HO/HONotes: Y55 is a prototrophic, homothallic diploid strain that was originally isolated by Dennis Winge. Many auxotrophic mutant derivatives have been created by John McCusker by using ethidium bromide treatment to eliminate。

单细胞转录组中基因集打分

单细胞转录组中基因集打分

单细胞转录组中基因集打分英文回答:Gene Set Scoring in Single-Cell Transcriptomics.Gene set scoring (GSS) is a widely used method for analyzing single-cell transcriptomics data. GSS allows researchers to identify biological pathways or gene sets that are enriched or depleted in specific cell populations or experimental conditions.There are several different approaches to GSS, but the most common is the "Kolmogorov-Smirnov test". This test compares the distribution of expression values for a gene set to the distribution of expression values for all genes in the dataset. A Kolmogorov-Smirnov statistic is calculated, which measures the maximum difference between the two distributions. A low Kolmogorov-Smirnov statistic indicates that the gene set is not enriched or depleted in the cell population or experimental condition, while a highKolmogorov-Smirnov statistic indicates that the gene set is enriched or depleted.GSS can be used to identify biological pathways that are associated with different cell types, disease states, or experimental conditions. It can also be used to identify gene sets that are differentially expressed between different cell populations or experimental conditions.中文回答:单细胞转录组中的基因集打分。

统计学随机分组(实验动物含小鼠)-PPT

统计学随机分组(实验动物含小鼠)-PPT
[4] 赵伟,孙国志. 常用实验动物随机分组方法,实验动物,2009年 第4期
[5]万霞, 刘建平. 临床试验中的随机分组方法. 中医杂志2007 年第 48 卷第3 期
阳性对照组:通常是给予疾病模型动物已知应 该有效的药物或者其他有效因素的处理;目的 是对比某受试药物与阳性药物对比,是否有效; 举例:已知阿霉素对乳腺癌肿瘤生长有抑制作 用,因此我们可以选用阿霉素作为阳性对照, 来判断此受试药物的治疗效果。
问题二: 每组几只合适?
动物实验设计应遵循实行“3R原则”,包括实验 动物的替代、减少和优化原则,其中减少即指 尽量减少实验动物的数量。查阅文献,并未发 现对实验动物数目有绝对要求,但在减少的同 时,一定要满足统计学要求。统计学上要求一 般至少每组有6个可用数据,才有意义。
[2] Festing M F, Altman D G. Guidelines for the Design and Statistical Analysis of Experiments Using Laboratory Animals[J]. Ilar Journal, 2002, 43(4): 244-258.
一般小鼠的每组一般不少于10只; 一般大鼠每组不少于6只; 大动物等级越高,价格越贵,根据情况可适当
减少,但一般不能少于4-5只。
问题三: 如何进行随机分配?
根本不同的实验目的,实验对象,分组时常用的 方法有:完全随机设计、随机区组设计等[3-4]。 实现随机分组时需要利用随机数字表以及随机数 余数分组法。
优势:区间分组将保证了实验组间的生物差异减到了 最小,遵循了一致性原则与随机性原则,使实验结果 更具有统计学意义。
注:有时候我们会碰到多个因素,则需要依次分区, 如:40只体重差异较大的小鼠,雌雄不一,此时我们 不能只按照体重区组分配,需要先把雌雄分开,然后 各自按体重分层。即它要求随机先按某一因素分成相 等的几组,再在组内按另一因素分成几个区。
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BORG–MARCHENKO-TYPE UNIQUENESS RESULTS FOR CMV OPERATORS
STEPHEN CLARK, FRITZ GESZTESY, AND MAXIM ZINCHENKO Dedicated with great pleasure to Pavel Exner on the occasion of his 60th birthday Abstract. We prove local and global versions of Borg–Marchenko-type uniqueness theorems for half-lattice and full-lattice CMV operators (CMV for Cantero, Moral, and Vel´ azquez [15]). While our half-lattice results are formulated in terms of Weyl–Titchmarsh functions, our full-lattice results involve the diagonal and main off-diagonal Green’s functions.
R
dρ(λ)[(λ − z )−1 − λ(1 + λ2 )−1 ],
z ∈ C\ R,
(1.2)
Theorem 1.2 is equivalent to the following statement: Denote by dρj the spectral measures of Hj , j = 1, 2. Then dρ1 = dρ2 implies V1 = V2 a.e. on [0, ∞). (1.3)
arXiv:0803.3175v1 [math.SP] 21 Mar 2008
1. Introduction To set the stage, we briefly review the history of Borg–Marchenko-type uniqueness theorems. Apparently, it all started in connection with Schr¨ odinger operators on half-lines, and so we turn to that case first. d2 1 Let Hj = − dx 2 + Vj , Vj ∈ L ([0, R]; dx) for all R > 0, Vj real-valued, j = 1, 2, be two self2 adjoint operators in L ([0, ∞); dx) which, just for simplicity, have a Dirichlet boundary condition at x = 0 (and possibly a self-adjoint boundary condition at infinity). Let mj (z ), z ∈ C\R, be the Weyl-Titchmarsh m-functions associated with Hj , j = 1, 2. Then the celebrated Borg–Marchenko uniqueness theorem, in this particular context, reads as follows: Theorem 1.1. Suppose m1 (z ) = m2 (z ), z ∈ C\R, then V1 (x) = V2 (x) for a.e. x ∈ [0, ∞). (1.1) This result was published by Marchenko [47] in 1950. Marchenko’s extensive treatise on spectral theory of one-dimensional Schr¨ odinger operators [48], repeating the proof of his uniqueness theorem, then appeared in 1952, which also marked the appearance of Borg’s proof of the uniqueness theorem [11] (apparently, based on his lecture at the 11th Scandinavian Congress of Mathematicians held at Trondheim, Norway in 1949). As pointed out by Levitan [43] in the Notes to Chapter 2, Borg and Marchenko were actually preceded by Tikhonov [68] in 1949, who proved a special case of Theorem 1.1 in connection with the string equation (and hence under certain additional hypotheses on Vj ). Since Weyl–Titchmarsh functions m(z ) are uniquely related to the spectral measure dρ of a self-adjoint (Dirichlet) Schr¨ odinger d2 2 + V in L ([0 , ∞ )) by the standard Herglotz representation operator H = − dx 2 m(z ) = Re(m(i)) +
1
2
S. CLARK, F. GESZTESY, AND M. ZINCHENKO
In fact, Marchenko’s proof takes the spectral measures dρj as the point of departure while Borg focuses on the Weyl–Titchmarsh functions mj . We emphasize at this point that Borg and Marchenko also treat the general case of non-Dirichlet boundary conditions at x = 0 (in which equality of the two m-functions also identifies the two boundary conditions); moreover, Marchenko also simultaneously discussed the half-line and the finite interval case. For brevity we chose to illustrate the simplest possible case only. To the best of our knowledge, the only alternative approaches to Theorem 1.1 are based on the Gelfand–Levitan solution [20] of the inverse spectral problem published in 1951 (see also Levitan and Gasymov [44]) and alternative variants due to M. Krein [41], [42]. For over 45 years, Theorem 1.1 stood the test of time and resisted any improvements. Finally, in 1998, Simon [59] proved the following spectacular result, a local Borg–Marchenko theorem (see part (i) below) and a significant improvement of the original Borg–Marchenko theorem (see part (ii) below): Theorem 1.2. (i) Let a > 0, 0 < ε < π/2 and suppose that |m1 (z ) − m2 (z )| along the ray arg(z ) = π − ε. Then V1 (x) = V2 (x) for a.e. x ∈ [0, a]. (ii) Let 0 < ε < π/2 and suppose that for all a > 0, |m1 (z ) − m2 (z )| along the ray arg(z ) = π − ε. Then V1 (x) = V2 (x) for a.e. x ∈ [0, ∞). (1.7)
1991 Mathematics Subject Classification. Primary 34E05, 34B20, 34L40; Secondary 34A55. Key words and phrases. CMV operators, orthogonal polynomials, finite difference operators, Weyl–Titchmarsh theory, Borg–Marchenko-type uniqueness theorems. Based upon work supported by the US National Science Foundation under Grants No. DMS-0405526 and DMS0405528.
|z |→∞
1/2
|z |→∞
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