Axiovision软件的默认参数
激光共聚焦系统使用说明 Imager Vario LSM
共聚焦显微镜操作入门指南(仅供内部使用)文档作者：李宇(liyu@) 日期：2012-12-04文档校对：李宇日期：2012-12-04卡尔蔡司中国版权所有不得复制目录1开机 (1)1 .1接通总电源 (1)1 .2打开激光器 (1)1 .3打开控制器、主控电脑 (1)2使用激光共聚焦扫描软件Zen 2010 (2)2 .1打开软件 (2)2 .2切换到明场观察模式（目镜筒） (2)2 .3放入样品并在明场模式下找到焦平面 (3)2 .4切换到共聚焦扫描模式 (6)2 .5设置激光扫描参数，找到样品最亮的焦平面位置 (6)2 .6设置Z-stack扫描上下限 (8)2 .7开始扫描 (10)2 .8分析扫描结果，进行三维观测 (11)3关机 (15)4附：目镜中，使用明场、暗场和偏光模式观察样品 (16)4 .1明场模式 (16)4 .2暗场模式 (17)4 .3偏光模式 (17)5附：使用相机（CCD）拍摄明场、暗场和偏光图 (18)5 .1拍摄明场图 (18)5 .2拍摄暗场图 (19)5 .3拍摄偏光图 (19)1 开机1 .1接通总电源图 1 从左至右依次为：墙体总电源、稳压器电源、激光器和电脑电源1 .2打开激光器图 2 转动激光器钥匙，打开激光器，LED指示灯亮1 .3打开控制器、主控电脑图 3 依次打开左图显微镜主机控制器电源、右图电脑主机电源提示：当仅使用CCD拍图，或者长时间不用机器时，建议关闭激光器，以延长其寿命。
2 使用激光共聚焦扫描软件Zen 20102 .1打开软件双击图标，然后点击“Start System ”进入软件。
2 .2切换到明场观察模式（目镜筒）2 .2.1 在共聚焦软件中切换为明场观察模式：点击“Locate ”标签，选择“Online ”，点击“BF ”（Bright Field 的缩写）。
此时系统打开卤素灯，并将明场光学模块转入光路。
图 4 切换为明场观察模式提示：如果出现硬件通讯问题，软件左下角会弹出信息对话框，此时一般的解决方法是：1）重启Zen 软件；2）如果仍无效，关闭整个系统，过5分钟后再重启系统。
蔡司扫描仪软件版本查询指南说明书
What Version of Zen am I running?This quick-guide is intended to help you find specific software version Information we often need to best assist you.NOTE- In some cases is possible that you have both Zen Blue and Black software packages installed.Our Laser Scanning System’s Software is Zen Black for all modern systems,EXCEPT the LSM 800 which uses a specialized Zen Blue 2.1 software.Our Camera based widefield systems , Spinning disk, TIRF systems may use Zen Blue OR Zen Core (A specialized Materials focused Workflow-Based version of Zen Blue) Zen Blue has 2 different info tabs we need information fromThe directions that follow are where to locate the key information for most Zen VersionsZen Black has a gray icon and when launched and has a prominent gray bar at the top of the application window.Zen Black has one information area in the about areaThe one key area with specific version info in Zen Black is foundby going to the Help>About Menu.You can take a note, picture or do a screen grab to send theresulting About window information to support if requested todo so.The About info dialog that comes up should appear similar toshown:The key information we need is:Version #ConfigurationDongle NumberZen Core has a Square Blue (dark blue) icon on your desktop and the top of the software will probably look gray when launched. There are 2 key areas to get the information required. 1.)To get specific version info go to the top right Workspace customization icon and click it.In the resulting dialog simply click on About………from here follow from Zen Blue Step 3… next page.For Zen BlueZen Blue has a round Blue (dark blue) icon on your desktop and the top of the software should have a Blue color. There are 2 key areas to get the information required.2.)To get specific version info go to Help>About Zen3.)In the About Zen window > Choose - Show Zen Information at the bottom.4.)In the resulting Application Information window, navigate to the Software tab and note the version value.5.)Then go to the Service Packs tab to see what hotfixes have been applied.Send both the Software version and Service Pack (hotfixes Info.The key information is:Version Value (from Software tab)HotFix Names (from Service Packs tab)NEVER install/update a newer version of Zen/AxioVision software on an actual microscope system’s PC without our assistance. You could easily and unintentionally uninstall an existing good installation!If you have questions or comments please contact your local Zeiss sales consultant, application specialist, service technicianorourapplicationsupportteamat:1-800-509-3905/*****************。
XVID 1.0 参数详解21b
XVID 1.0关键参数详解 2.1b目录：1.前言 (1)2.主界面 (2)3.Profile标签 (5)4.2nd PASS设定 (7)5.Motion标签 (8)6.Quantization标签 (9)7.DEBUG面板 (10)8.后记 (10)9.附录 (11)10.更新记录 (15)前言本文参照的XviD版本是XviD1.1.0 Beta12.1版修订说明：最近XviD更新到了1.1版，界面个功能都有了较大的改进。
本次更新将文章中的截图更新，按照功能的变动微调了文章结构，并且将一些功能的解释按照官方的说法进行了修正。
广告时间本人的小站开张了！地址是：/nmm，论坛是：/nmmbbs。
主题当然是MPEG4相关的啦，会经常发表一些我原创的或者我搜集来的好文章~各位有时间多去捧捧场~谢谢！一．主界面主界面Profile @ Level——这个选项包含一些预设值，与分辨率和码率有关，按旁边的“more...”按钮再切换到Level标签可以看到预设的数值。
因为我们制作的视频并不在其它视频播放设备（比如具备MPEG4解码能力的DVD播放机）中播放，无须考虑设备兼容性，所以这个选项一般可选“不限制”（unrestricted）或者默认的“AS @ L5”。
Zones——XviD1.0以前的版本都有一个参数叫Credit，Zones继承并加强了以前的Credit功能，使之可以控制片中任意一段视频。
大家知道，电影、动画片尾（或片头）都有制作人员名单（即Credit，下同），而且一般没人会看。
而老版XviD中Credit选项的作用就是，允许用户使用较低的质量制作Credit部分，让出更多的码率分配给正文部分，以提高正文部分的质量。
而在最新的XviD1.0中，这个Credit选项却消失了，取而代之的是一个新的参数——Zones。
在这里，我们可以把Zones理解成“区间”、或者说“片断”。
也就是说，我们可以在逻辑上将片子分成许多段，并给这些段落一些“特殊待遇”。
激光共聚焦系统使用说明 Imager Vario LSM
共聚焦显微镜操作入门指南(仅供内部使用)文档作者：李宇(liyu@) 日期：2012-12-04文档校对：李宇日期：2012-12-04卡尔蔡司中国版权所有不得复制目录1开机 (1)1 .1接通总电源 (1)1 .2打开激光器 (1)1 .3打开控制器、主控电脑 (1)2使用激光共聚焦扫描软件Zen 2010 (2)2 .1打开软件 (2)2 .2切换到明场观察模式（目镜筒） (2)2 .3放入样品并在明场模式下找到焦平面 (3)2 .4切换到共聚焦扫描模式 (6)2 .5设置激光扫描参数，找到样品最亮的焦平面位置 (6)2 .6设置Z-stack扫描上下限 (8)2 .7开始扫描 (10)2 .8分析扫描结果，进行三维观测 (11)3关机 (15)4附：目镜中，使用明场、暗场和偏光模式观察样品 (16)4 .1明场模式 (16)4 .2暗场模式 (17)4 .3偏光模式 (17)5附：使用相机（CCD）拍摄明场、暗场和偏光图 (18)5 .1拍摄明场图 (18)5 .2拍摄暗场图 (19)5 .3拍摄偏光图 (19)1 开机1 .1接通总电源图 1 从左至右依次为：墙体总电源、稳压器电源、激光器和电脑电源1 .2打开激光器图 2 转动激光器钥匙，打开激光器，LED指示灯亮1 .3打开控制器、主控电脑图 3 依次打开左图显微镜主机控制器电源、右图电脑主机电源提示：当仅使用CCD拍图，或者长时间不用机器时，建议关闭激光器，以延长其寿命。
2 使用激光共聚焦扫描软件Zen 20102 .1打开软件双击图标，然后点击“Start System ”进入软件。
2 .2切换到明场观察模式（目镜筒）2 .2.1 在共聚焦软件中切换为明场观察模式：点击“Locate ”标签，选择“Online ”，点击“BF ”（Bright Field 的缩写）。
此时系统打开卤素灯，并将明场光学模块转入光路。
图 4 切换为明场观察模式提示：如果出现硬件通讯问题，软件左下角会弹出信息对话框，此时一般的解决方法是：1）重启Zen 软件；2）如果仍无效，关闭整个系统，过5分钟后再重启系统。
Axio Vision
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生成的图像显示在图像区，在 这里可以用播放器对进行操作。 该图象还未被保存，这时可以 看见图象名字是*号。保存图象。
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多色荧光拍摄
15. Channel Pool 中默认设置为自动
曝光。如果需要手动设定各通
道的曝光时间，在Exposure下，
选择Fixed模式。
点击Close Pool关闭 Channel Pool窗口。
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多色荧光拍摄
11. 注意：软件设置中会保留激活 属性页时形成的第一个空白通 道，请激活标记为1的属性页， 点击remove删除此不必要的通道。 在最后一个荧光通道的After acquisition…下拉菜单中选择FL off，在实验结束后关闭激发光。
– 荧光图像通常向下移动曲线；可见光图像通常向上移动曲线
拍摄荧光图像的调节
• 调节完成后，打开彩色模式。
• 系统提供多种伪彩色，可自由选择
添加比例尺
• 在工具栏点击比例尺快捷键，在图像上拖出所需 长度即可
图片叠加
• 如需进行多张荧 光图像的叠加， 可在Processing菜 单中选择Utilities 下的Add Channels。
图像的保存与输出
• 图像拍摄完成后，建 议保存为原始*.zvi格 式。
• 经过处理的tif或jpg图 像，如调节灰度，建 议另存为*.tif或jpg图像。 • 也可以使用export，输 出成所需格式。在 export对话框中，可以 完成批处理。
多色荧光拍摄 ——图像叠加
• 在工具栏添加通道快捷方式中叠加两幅图像即可。
AXIS-Q6035E基本调试说明
axis（安讯士）Q6035-E IPC快球摄像机软件IPUtility 可以快速访问在线IPC 默认登录用户名：root 密码：pass 亦可以直接通过WEB方式直接登录IPC点击右侧上方“Setup”链接进入调试界面：如图所示：左侧树形目录常用选项：Basic Setup（基本设定）TCP/IP 为IPC设定IP地址Date & Time 时间设定Video Stream 图像基本参数其中：Overlay Settings 选项中为画面叠加设定：Include date勾选即为显示日期，Include time勾选即为显示时间Include text: 填写显示内容（目前摄像机位英文版本，不支持汉字，填入#i 可以显示本机IP，#B可以显示实时码流，如#i #B可以同时显示以上内容，建议加入空格键以便区分，不可以加入标点。
）Video（图像）Camera Settings 为IPC画面详细设定：Color level:（色彩）建议55Brightness:（亮度）建议55Sharpness:（明锐度）建议50Wide Dynamic Range（WDR）宽动态设定可以根据实际情况选择不同级别宽动态，注意设定宽动态增加IPC码流。
对白天效果亦会产生影响。
需要精心调试。
Exposure Settings除非特殊情况，最好默认设定，包含Max exposure time曝光Max gain:增益数值越大画面越亮，夜间适当调高可以增加画面亮度，但遇到强光可能出现曝光。
需要适当调节。
其中IR cut filter（滤光片）选项开启夜视会转为彩色，但噪点会增加。
Live View Config中Stream Profile选项选择H.264 则WEB访问IPC时为H.264，PTZ（控制）Preset Positions 选项中为预设位设定，把画面调到适当位置，在Current position:中命名位置名称，并勾选Use current position as Home，点击add按钮即可设定完毕，此项完成后可以实现掉电复位。
AxioVision 4.X.X Scalings说明书
The purpose of this document is to introduce the concept of Scalings and guide the user through creating and using Scalings with AxioVision 4.X.X. Any questions or concerns can be directed to the ZEISS Product and Application Support group at 1-800-509-3905 or *****************What are Scalings?The term Scalings describes the information the software needs to determine the width and height of individual pixels (“picture elements”) in a digital microscope image. In the case of Z-stack images, there is an additional scaling dimension: the “thickness” of an individual slice and the “voxels” ("volume elements”) have a third dimension in addition to width and height, i.e. thickness.Why are Scalings needed?Scaling information allows you to calculate the size of an image. For example the image width equals the number of pixels in x-direction [pixels] multiplied by the pixel size in x-direction [μm / pixel] and the image height equals the number of pixels in y-direction [pixels] multiplied by the pixel size in y-direction [μm / pixel].In addition to calculating the overall size of an image, scaling information allows the user to measure structures in an image and to assign scale bars.Without the pixel sizes, AxioVision is unable to acquire MosaiX images since the motorized scanning stage needs accurate information on the size of acquired images in order to scan and correctly align the images of a given area.Finally, scaling information is required for deconvolution processing.What are the system components that determine Scalings?The pixel size of a digital microscope image is determined by the following microscope components and camera parameters:1)The magnification of the objective used.2)The magnification factor of the optovar/tube lens or the optovar cube in the reflector turret.3)The magnification factor of the camera adapter(s) used.4)The pixel size of the digital camera’s CCD sensor.5)The binning mode used on the digital microscope camera.6)And finally, in the case of a stereomicroscope, the zoom factor.How do I acquire Measured Scalings?Using the AxioVision Scalings Wizard, you can acquire images of a stage micrometer and calculate the sizes of the pixels for each of the images, for all available objectives, optovar / tube lens or zoom settings, and camera modes. Each of these definitions can be saved as a Scalings file. Scalings generated this way are referred to as Measured Scalings.Start by creating images of a stage micrometer using all of the different system component configurations for which you wish to create Scalings. You can save these images in a dedicated folder or leave them open in AxioVision.1.Select Measure and Scalings from the menu bar, or select Scalings and Advanced fromthe Workarea.2. The Scalings Control window displays all the available Scalings for your user account. SelectNew to start the Scalings Wizard.3. If you have a saved micrometer image taken using a system component configuration forwhich you wish to create Scalings, click File to select the image. If the image is open inAxioVision, click Gallery . Once the appropriate image is loaded, hit Next.Each division = .01mm4.Select the Single Distance method in the next window. Hit Next.5.Draw a line between two points on the image and enter that known distance in Assignrange: X-Direction. AxioVision will calculate the pixel length using that information.Each division = .01mmProperly drawn lineImproperly drawn line6.Click Finish. Enter a name for the Scaling in the window that pops up and click OK.7.Repeat Steps 1-6 for all other system component configurations for which you want Scalings. Scalings are saved in the Scaling folder (C:\Users\Username\Documents\Carl Zeiss\Data\Scaling) as files with file extension .zvsc. You can make a Scaling available for all users by moving the file to the C:\Users\All Users\Documents\Carl Zeiss\Data\Scaling folder. Each user will then be able to see a list of these Scalings in his or her Scalings Control window.How do I acquire Theoretical Scalings?AxioVision always checks whether a valid Measured Scaling file is available and, if so, uses this file. If you have no Measured Scalings, for certain configurations or no Measured Scalings altogether, you can still apply Scalings to your images. AxioVision can extrapolate Scalings from existing Measured Scalings and/or calculate them using theoretical data.1.If your system components are motorized and/or encoded, AxioVision can automaticallycalculate Theoretical Scalings, provided all the system components that determine Scalings (page 2) are configured in MTB20XX Configuration X.X.X.X (MTB).2.If some/all the system components that determine Scalings (page 2) are manual, configureMTB with an Other Stand and all the relevant system components.3.In the case that you have created a Measured Scaling for a certain system configuration butwant to use a somewhat different configuration without creating a new Measured Scaling(e.g. adding camera binning or changing an objective to a system configuration that isotherwise identical), AxioVision can extrapolate a Theoretical Scaling (e.g. for that objective magnification or the generated binned image). The Measured Scaling off which anextrapolated Theoretical Scaling is created is marked with a green dot in the ScalingsControl window to indicate that it is a “parent” of a dependent Scaling.How can I enable Automatic Scalings?AxioVision can automatically apply the appropriate Measured Scaling or Theoretical Scaling to a given system configuration. This is referred to as Automatic Scaling.1.If your system components are motorized and/or encoded and configured correctly in MTB,AxioVision can automatically apply the appropriate Scaling to a given configuration. Enable the Automatic Scaling option through Measure from the menu bar or through theAutomatic scaling checkbox in the Scalings Control window.2.If AxioVision does not automatically recognize all system parameters and/or if some/all thesystem components that determine Scalings (page 2) are manual, you can manually apply a Scaling to a given configuration. Click on the appropriate Scaling and hit Activate selection in the Scalings Control window. The green checkmark move to the selected Scaling toindicate that this Scaling is the active Scaling that will be applied to all subsequent image acquisitions.3.You can apply a Measured Scaling to an image that has already been acquired. Thisoverwrites the image’s previous Scaling but does not affect the Automatic scaling orActivate selection settings. This is not advisable if you are not certain about theconfiguration used to acquire the image and that the Scaling applied accurately reflects this configuration.Open the image and navigate back to the Scalings Control window. Hit Apply selection to image.How can I change the unit of measurement?Select Measure and Scalings from the menu bar. Select the appropriate unit of measurement from the drop down menu at the bottom of the Scalings Control window. Click Apply selection to image to apply the change to the active image; click Active selection to apply the change to subsequent image acquisitions.Note: You can only change the unit of measurement of an image with this method when using the same system computer used to acquire the image. If an image is opened in AxioVision on a different system computer or on an offline workstation, you must use Offline Scalings to change the unit of measurement.How can I generate Offline Scalings?If you acquire an image without Scalings, you can assign Scalings afterwards provided you know all the system components that determine Scalings (page 2) that were used during acquisition. Please note that this method is not recommended, especially for systems with third party components.1.Download:ftp:///PUBLIC/Application_Support/Offline_Scaling_Calculator.xlsxThe username and password should be in the email in which this document was sent.2.Follow the steps for acquiring Measured Scalings (pages 3-7), but load the image for whichyou wish to create offline scalings (step 3), draw a line of arbitrary length in the image (step5), and enter “1” in the Distance field.e the Excel spreadsheet to determine the pixel size for the image by selecting theappropriate camera, camera adapter, objective lens, Optovar, and camera binning. Dividethe number calculated from the spreadsheet (μm/pixel) by the Factor (Micrometer/Pixel)displayed in the Scalings Wizard (step 5). Type the result in the Distance field and hit Enter.The Factor now displayed in the scaling wizard should equal the one calculated using thespreadsheet. Finish step 6.4.The created scaling will now appear on the Scalings Control window and can be applied tothe image by selecting the Scaling and clicking Apply selection to image.Why does AxioVision throw a scaling-related error upon startup?If the Automatic Scaling function is enabled, AxioVision monitors the system settings and displays this message if a valid Scaling is missing. This occurs when Scalings components are missing from or improperly configured in MTB; when AxioVision is started before the microscope is powered on; when the microscope does not communicate with MTB and, therefore, with the software. Check that the microscope is powered on, and that it is communicating with the software by selecting the microscope stand in MTB and clicking Check Connection. Restart AxioVision.When this happens, a Scaling file with the name “Pixel” becomes active in the Scalings Control window.。
AxioVision简易操作说明
德国蔡司AxioVision图像拍摄及处理软件
简易操作说明
1.在显微镜目镜中找到需要拍摄的样品，聚焦清晰。
2.将显微镜转到相机拍摄模式。
3.点击
4.点击菜单中
5.点击
6.点击
7. 点击
8. 放大倍数。
Snap拍摄图像。
10. 右键点击图像菜单中Auto Zoom可将图片调整至充满整个窗口。
11. 点击菜单中可
右键点击图像，在Peoperties菜单下Attributes中可更改标尺及文本框格式。
12. 点击保存图像。
保存为ZVI格式可保存所有图像信息，且附加的标尺及文本信息可以修改。
保存为其它格式且勾选Burn-in annotations时，附加信息将覆盖原始图像信息。
不勾选Burn-in annotations时保存原图，无附加信息。