The millimetre variability of M81 -- Multi-epoch dual frequency mm-observations of the nucl
翻译 1

Mg-Al-Zn合金组织的晶粒尺在摩擦搅拌的依赖性弱搅拌处理的Y.N.王,a,b C.I.张,a C.J.李,a H.K.林a,c和黄祚芊a,*a材料科学与工程学院;纳米科学中心和纳米技术,国立中山大学圣大学,高雄804,台湾,中华民国b材料科学与工程学院,大连理工大学,大连116024,中国研究所c工研院南,工业技术研究院,台南县734,台湾,中华民国收到2006年4月25日;经修订的2006年5月18日;接受二零零六年六月七日可在网上二零零六年七月五日摘要在Mg-Zn-Al系合金热挤压加工通常表现出较强的粒度屈服应力的依赖。
然而,相同的摩擦搅拌处理的Mg-Zn-Al系合金的样品表现出弱得多的晶粒尺寸的依赖。
高施密特因子摩擦搅拌处理的样品在0.3左右,负责在的Hall-Petch关系的低参数。
关键词:镁合金;搅拌摩擦加工;纹理;晶粒尺寸的依赖镁合金已经吸引了运输车辆制造越来越大的兴趣,因为它们可以提供一个相当大的重量减少的结构。
然而,他们的延展性较差,由于在室温下的六边形结构可滑移系的数量有限,可能会限制其广泛应用。
另一方面,在镁合金的晶粒尺寸强化效率比以Al和其它合金高得多[1],这意味着晶粒细化镁合金是更有利。
大量的研究集中在镁合金的微结构上的修改已经进行,以提高和控制的机械性能[2-12]。
在镁合金中,存在基础和非基础滑移系之间的临界剪切应力(CROSS)有很大差异[13],这引起了严重的各向异性的机械性能。
其结果是,当变形镁合金具有强的晶体学织构在其微观结构,它们的机械性能显著由质地除了晶粒尺寸的影响[4-7,12,14]。
最近,研究搅拌摩擦加工(FSP)已经证明,有效的微观组织均匀化和细化可在镁基合金可以实现为严重的塑性变形和动态再结晶的结果。
已经发现该纹理具有强烈的不均匀分布沿着焊接工具的销柱表面基面的积累也带出,在搅拌区[8,15]。
有大量的报道[6,16-18]对晶粒尺寸和镁合金的基础上的Hall-Petch关系机械性能的关系之间的相关性。
人教版四年级语文下册《读课文 3 新奇的纳米技术(2018年1月安徽第1次印刷)》导读课_1

1 m
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红血球:200-300nm
细菌: 200-600nm
胃幽门 螺杆菌
病毒: 30-100nm
纳米粒子与病毒大小相当
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2、什么是纳米科技?
最早提出纳米尺度上科学和技术问题的 是美国著名物理学家、诺贝尔奖金获得者 理查德·费曼(Richard P Feynman)。
• 1959年费曼在一次题为《在底部还有很大空间》(“There is Plenty of Room at the Bottom. ”)著名的演讲中提出 “如果有 一天能按人的意志安排一个个原子和分子,将会产生什么样的奇 迹呢?”并预言,说人类可以用新型的微型化仪器制造出更小的 机器,最后人们可以按照自己的意愿从单个分子甚至单个原子开 始组装,制造出最小的人工机器来。可以说这些都是纳米技术的 最早的动意/梦想。
“纳”(nano)来自于希腊文,本意 是“矮子”或”侏儒”(dwarf)的意思。
纳米结构: 通常是指尺寸在100纳米以下(1-100nm) 的微小结构。
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一纳米有多小?
Human Hair
100 m
Take 1 slice
Human Hair
100 slices
Take 1 slice
1nm 1000 slices
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纳米科技的最终目的:是按人的意愿,操纵单个原子、分子, 构建纳米级的具有一定功能的器件或产品。
纳米科技研究的技术路线:可分为“自上而下”和“自下而上” 两种方式。
“自上而下(top-down)” —— 是指通过微加工或固态技术, 不断在尺寸上将人类创造的功能产品微型化 (传统技术);
传统的生产模式,是从大到小,从上到下的,比如造一个橱,要从粗木 到木材到成品;如造电脑,从半导体硅片,经过切、割、刻、蚀、渗杂 等许多步骤,变成电子元器件,这个过程,原材料浪费大、能耗大。
+Development of an intracavity-sumed multiple-wavelength Nd YAG laser for a rugged, solid-state sodi

Development of an intracavity-summedmultiple-wavelength Nd:YAG laser for a rugged,solid-state sodium lidar systemR.W.Farley and P.D.DaoA single-cavity solid-state laser that is resonant with sodium D2absorption is reported.Simultaneous1.06-and1.32-µm emission from a Q-switched Nd:YAG laser is summed with an intracavity type II KTPcrystal.A single-intracavity e´talon is sufficient to provide a time-averaged linewidth of1.7GHz at589nm.Key words:Sodium lidar.1.IntroductionSimultaneous lasing at multiple wavelengths with a common active medium has been reported by numer-ous investigators,using both solid-state and dye laser materials.1–3Bethea reported4summing the1.32-and1.06-µm emissions of a single Nd:YAG crystal that was simultaneously lasing at these wavelengths, producing589-nm output.His arrangement used overlapping,collinear cavities for the two wave-lengths,a common Q switch,and an extracavity KD*P crystal to mix the outputs.Jeys5subse-quently reported a summed Nd:YAG laser capable of resonantly exciting the atomic sodium D2transition. This laser used separate cavities and gain media for the production of synchronously mode-locked beams, which were externally summed.Yellow output was obtained from an intracavity-summed Nd:YAG by researchers at Lincoln labs,6but details were unpub-lished.In this paper we present results of frequency-summed emission obtained with a single cavity and gain medium.Development of a Nd:YAG laser with a single crystal,cavity,and Q switch that could provide a rugged,compact solid-state device capable of reso-nantly exciting atomic sodium appears promising.A reliable,easily aligned,transportable solid-state laser for sodium lidar is desired to facilitate investiga-tions of high-altitude atmospheric dynamics for im-proved neutral atmospheric modeling.Frequency mixing with a nonlinear optical crystal inside the oscillator exploits higher intracavity intensities and ensures a high degree of spatial overlap between the beams.The high damage threshold,acceptance angles,and nonlinear coefficients of KTP,which make it the material of choice for intracavity-doubled,Q-switched Nd:YAG lasers,suggested its use for this application.2.ExperimentAflash-lamp-pumped Nd:YAG crystal in a Q-switched oscillator that incorporates an intracavity KTP crys-tal produces589-nm output that is resonant with the absorption of atomic sodium.The oscillator is tuned by means of a thick13-mm2fused silica intracavity e´talon and through control of the cooling-water tem-perature,7yielding a stable,time-averaged linewidth of1.7GHz.Resonance with the sodium transition is established by observation of the optogalvanic output of a sodium hollow-cathode lamp.The0.635cm310.16cm Nd:YAG crystal is pumped by means of aflooded,close-coupled,diffuse-reflecting cavity provided by Big Sky Laser1Bozeman, Mont.2.The pump cavity is water cooled by a recircu-lating chiller.A heating coil spliced into the coolant return line shifts the summed emission into reso-nance with sodium.The optimum coolant tempera-ture is approximately50°C,although this value is not critical.The dual xenonflash lamps are simmered, and the main discharge is provided by a single-mesh pulse-forming network charged with an Analog Mod-ules controller.R.W.Farley is with PhotoMetrics Inc.,4Arrow Drive,Woburn, Massachusetts01801-2040.P.D.Dao is with Phillips Laboratory, OL-AA@GPIM,29Randolph Road,Bedford,Massachusetts 01731-3010.Received10June1994;revised manuscript received11October 1994.0003-6935@95@214269-05$06.00@0.r1995Optical Society of America.An 8mm 38mm 35mm 1height 3width 3length 2hydrothermally grown KTP crystal cut at u 578°,f 50°was provided by Philips Components 1Saugerties,N.Y.2.This device permits type II critical phase matching for 1.064µm 1o 2and 1.319µm 1e 2,producing 589nm 1o 2.A high effective nonlinear coefficient 8of d eff 53.5pm @V ,and relatively small 1.1°walkoff angle of the 1.319-µm beam from the phase-matching direction are expected.A single electro-optic Q switch suffices to hold off lasing during the flash-lamp pump pulse.The switch subsequently opens,providing low losses at all operat-ing sing is first obtained at 1.06µm,with subsequent emission at 1.32µm,in 25-ns and 45–65-ns FWHM pulses,respectively.An 18-ns pulse at 589nm is obtained during the overlap of the IR emissions.At the summed frequency,only forward-propagating emission is observed.In addition to 1.32µm,lasing is simultaneously obtained at the higher-gain 1.34-µm line.As the corresponding phase match angle is only 0.2°less,this line may also be summed with 1.06µm if the KTP crystal is slightly tilted,resulting in 592-nm output that peaks 230ns after the Q switch fires.This is 20ns earlier than the 589-nm output under identical pumping condi-tions.The optical configuration is shown in Fig.1.The rear mirror is a 18-m radius-of-curvature high reflec-tor at both 1.32and 1.06µm.The Q switch consists of a Glan–Taylor polarizer and a KD*P Pockels cell,which is maintained at the quarter-wave voltage for maximum hold off at 1.06µm.A high-voltage field-effect transistor switch grounds the Pockels cell in less than 10ns to open the Q switch.Without the high voltage,the laser operates in long-pulse mode at 1.34µm only,and rough alignment of the cavity isaccomplished by minimization of the buildup time for the first relaxation oscillation spike.Temporal per-formance at 1.3µm was monitored with a germanium photodiode,and a silicon photodiode was used for all other wavelengths.The KTP crystal is located as close to the plano 1r 5`2output coupler and,consequently,as close to the beam waist as possible.The z –x plane is tilted 45°from vertical so the fundamentals propagate as both o and e components,thus only half of each fundamental may contribute to the type IIsummedFig.1.Optical layout of the simultaneous multiple-wavelength,intracavity-summed Nd:YAG laser.The stable resonator oscillator is composed of a 18-m rear reflector and a flat output coupler that reflects IR and transmits the summed emission.A dichroic reduces feedback at 1.06µm to make oscillation at 1.32µm possible.The locations of both alternative wave plates are indicated in the schematic,although only one was used at a time,and only data collected with the quarter-wave plate are presented.HR,high reflector;PH,pinhole.Fig.2.Normalized,integrated optogalvanic response of the sodi-um–neon hollow-cathode lamp as a function of e ´talon tilt.The squares represent lamp output for a given e ´talon position averaged over 16laser shots.The solid curve represents the Doppler profile of 300°K sodium atoms.Despite considerable scatter,the data indicate resonance with and a laser linewidth comparable with or narrower than the Doppler-broadened absorption.output.To compensate for depolarization by KTP when it is used in this orientation,the optic axis of a quartz wave plate,l@4at both1.064and1.319µm,is subsequently aligned parallel to the plane of polariza-tion of the fundamentals.9This effectively inter-changes the o and e components so that the depolariza-tion by the KTP is precisely undone on the reverse pass through the crystal.A45°dichroic mirror rejects approximately90%of the1.06-µm beams and less than2%of the1.3-µm beams per pass.The cavity is terminated by a 1.32-µm high reflector, which reflects approximately35%of the 1.06-µm beam and serves as the589-nm output coupler.An aperture just before this mirror provides a degree of transverse-mode selection.The589-nm output crosses a45° 1.06-µm high reflector and an80-Åinterferencefilter before it is analyzed by the hollow-cathode lamp,Burleigh pulsed laser spectrum analyzer,or monitor e´talon.The final pulse energy after the interferencefilter1nomi-nal50%transmission2is30–80µJ,depending primar-ily on lamp energy and timing of the Q-switch open-ing.To suppress feedback at1.06µm and unintentional e´talon effects,all intracavity surfaces were antireflec-tion coated for both1.06and1.32µm,except forthe Fig.3.Spectral analysis of laser performance for each of the wavelengths produced in this study,measured with a200-MHz resolution Fabry–Perot e´talon.The589-nm and592-nm signals were produced intracavity with KTP,and the others were externally doubled with KD*P.For each wavelength,a single-shot and a smoother200-shot average are superimposed.e´talon,mirrors,and Pockels cell,which is coated for only1.06µm.Once the operating temperature is attained,the frequency of the summed output is adjusted by one’s angle tuning3-mm fused silica e´talon with coatings that provide low reflectivity at 1.3µm and70%reflectivity at1.06µm.To facilitate analysis,an external type II KD*P crystal doubled each IR wavelength when it was tuned to the appropri-ate angle,producing more readily analyzed532-, 660-,and669-nm outputs.The doubler accepted reflections off the dichroic,which attenuated the 1.06-µm transition.3.ResultsAlthough the laser may typically be tuned several gigahertz by one’s tilting the e´talon,this process significantly varies walkoff losses,effective gain,and consequently the buildup time for the1.06-µm oscilla-tion.No such temporal variation is observed for the 1.3-µm emissions,although both doubled outputs at 660and669nm are spectrally shifted with the e´talon position.Thus tilting the e´talon not only tunes the emissions but also varies the relative timing between them.Ultimately no overlap between1.06and1.32µm is obtained,and no summed output is observed. This result may be compensated by slight variation of theflash-lamp energy162%2or cavity losses at1.06µm.The latter approach may be readily effected by the use of a wave plate that is l@1at1.32µm and l@2 at1.06µm and placed between the Nd:YAG and KTP crystals.When the polarization of the1.06-µm fun-damental is rotated nearly90°,the KTP crystal can be mounted normally,instead of at45°,as is required for type II interactions when the input polarizations are parallel.This arrangement allows the full intensi-ties of the fundamentals to contribute to the summed output.It also eliminates depolarization losses,and thus the need for the quarter-wave plate compensator. Slight rotation of the half-wave plate has a negligible effect on the1.32-µm radiation but greatly affects losses at 1.06µm,readily compensating for those induced when the e´talon is tilted.Although slightly lower lamp energies are required,no other significant differences were detected as a result of which method was used.The data presented in this paper were obtained with the quarter-wave plate compensator, with the KTP crystal mounted at45°.The optogalvanic response of a hollow-cathode lamp demonstrates resonance with the sodium D2 absorption.A plot of lamp output versus e´talon tilt is presented in Fig.2.The squares in Fig.2repre-sent normalized integrated lamp output averaged over16laser shots for each e´talon angle.The theoretical curve corresponds to the Doppler line-width for300°K sodium,which is considered a lower limit for the actual temperature of sodium atoms in the lamp.For each e´talon tilt,theflash-lamp energy was adjusted for maximum output at589nm.The data show reasonable agreement with the Doppler profile,which suggests only a relatively small contri-bution from the laser linewidth.Errors are primar-ily shot-to-shot intensityfluctuations at589nm and uncertainties in e´talon position that are due to the limited resolution of its mount.A more accurate relative measurement of laser spectral performance is provided by a wavemeter 1Burleigh PLSA-35002that incorporates230-GHz Fizeau and10-GHz free-spectral-range Fabry–Perot e´talons with enhanced silver coatings and CCD detec-tors.The latter e´talon provides200-MHz resolution, which is adequate for all laser outputs reported in this study.Wavelengths were computed with the Fizeau wedge,and linewidths and line shapes were mea-sured from the Fabry–Perot fringe patterns.Results of spectral analyses are presented in Fig.3for both single-shot and200-shot averages.The intracavity e´talon has a35-GHz free-spectral range and a reflectivefinesse of9at1.06µm,provid-ing a single-pass resolution of3.9GHz.This results in a360-MHz linewidth at532nm.By contrast the e´talon has a reflectivefinesse less than1at1.3µm. Compared with532nm,the660-nm and669-nm emissions are successively broader,resulting in summed linewidths in the same order,with589-nm output narrower than the output at592nm.Clearly the broad 1.3-µm emission poses the more severe limitation on the spectral performance of this system. Also,despite the antireflective coatings,strong modu-lation of the1.3-µm emissions is observed because of slight reflections from the ends of the Nd:YAG rod. 4.ConclusionsThe wavemeter and hollow-cathode lamp analyses demonstrate resonance with and a linewidth compa-rable with the Doppler-broadened sodium absorption. The laser provides a compact,stable,solid-state source for resonant excitation of sodium,which is tuned by one knob.The only other required adjustments are optimization of summing crystal orientation andflash-lamp power.Currently the energy obtained at589 nm represents only0.1–0.2%of the total output. The low summing efficiency could be greatly en-hanced by improvement of temporal overlap between the summed transitions and by better spectral control of the1.3-µm emission.For this laser to be part of a practical sodium lidar system,kilohertz repetition rates or at least an order of magnitude higher pulse energies are required.The summed linewidth is comparable with and limited by the1.32-µm emission.Seeding this tran-sition would greatly reduce the589-nm linewidth, discriminate against1.338-µm emission,and reduce the Q-switch buildup time for better overlap with 1.06-µm emission.Addition of a thin e´talon instead may also significantly improve performance,with much less additional bulk and complexity.The sim-plest modification,the use of mirrors with lower reflectivity at1.338µm,could suppress this parasitic transition and increase efficiency but would provide no improvement in linewidth at589nm.Varying the Pockels cell voltage to open the Q switch more slowly may also improve spectral quality and reduce intensityfluctuations of the589-nm emis-sion.Although the16-shot averages used for spec-tral analysis were relatively stable,shot-to-shot en-ergyfluctuations of630%were typical.This largely results from jitter in the timing of the 1.06-µm emission,which affects the overlap between the funda-mentals and thus the summed intensity.Both the temporal profile and intensity at1.3µm are very stable,essentially insensitive to e´talon position,and only slowly varying withflash-lamp energy. Although more sophisticated control of the Q switch requires only modest additional electronic complex-ity,it may prove a dubious modification unless addi-tional discrimination is provided for the1.338-µm transition.Although the mirrors are highly reflec-tive for1.32µm,the Q switch favors longer wave-lengths.As the Pockels cell is maintained at the quarter-wave potential for1.06µm,discrimination is highest for this wavelength and progressively less for 1.32and 1.34µm.Were the Pockels cell to be opened slowly,this could further favor the higher-gain,longer-wavelength emission,enhancing592-nm instead of589-nm production.This work was supported under U.S.Air Force contract F19628-92-C-0160.References1.R. A.Morgan, F. A.Hopf,and N.Peyghambarian,‘‘Dual-frequency Nd:YAG laser for the study and application of nonlin-ear optical crystals,’’Opt.Eng.26,1240–1244119872.2.H.Y.Shen,Y.P.Zhou,W.X.Lin,Z.D.Zeng,R.R.Zeng,G.F.Yu,C.H.Huang,A.D.Jiang,S.Q.Jia,and D.Z.Shen,‘‘Secondharmonic generation and sum frequency mixing of dual wave-length Nd:YALO3laser influx grown KTiOPO4crystal,’’IEEE J.Quantum Electron.28,48–51119922.3.Y.Saito,T.Teramura,A.Nomura,and T.Kano,‘‘Simulta-neously tunable three-wavelength dye laser,’’Appl.Opt.24, 2477–2478119852.4. C.G.Bethea,‘‘Megawatt power at1.318µin Nd31:YAG andsimultaneous oscillation at both1.06and1.318µ,’’IEEE J.Quantum Electron.QE-9,254119732.5.T.H.Jeys,‘‘Development of a mesospheric sodium laser beaconfor atmospheric adaptive optics,’’Lincoln Lab.J.4,133–149 119912.6.P.Schultz,MIT Lincoln Laboratories,Lexington,Mass.021731personal communication,19932.7.J.Marling,‘‘1.05–1.44µm tunability and performance of the cwNd31:YAG laser,’’IEEE J.Quantum Electron.QE-14,56–62 119782.8.H.Vanherzeele and J.D.Bierlein,‘‘Magnitude of the nonlinear-optical coefficients of KTiOPO4,’’Opt.Lett.17,982–984119922.9.K.C.Liu and M.G.Cohen,‘‘High-power Nd:YAG laser at532nm using intracavity type11KTP,’’in Digest of Conference on Lasers and Electro-Optics1Optical Society of America,Washing-ton,D.C.,19862,p.110.。
(3)--2010级细胞生物学期末试卷-参考答案-20130116

中山大学生科院细胞生物学期末试卷(A卷)(2010级生物科学、生物技术、生技应用、生态学、逸仙班等共280人)任课教师:王金发王宏斌考试时间:2013年1月16日上午9:30~11:30考场:东校区E101,E201课室参考答案一、填空题(每空2分,共10分)1、在M期可能有一种是染色体压缩包装的“打包工”的存在。
2、透明、细胞数量少,便于绘制细胞谱系图。
3、条件致死突变,在非致死条件下得到的突变体只影响分裂而不致死。
4、志愿者。
5、“心中有数”。
6、没有提升到假说及理论高度。
7、Truth is not a citadel of certainty to be defended against error; it is a shady spotwhere one eats lunch before tramping on.8、“天网恢恢疏而不漏”。
9、排球队中的一流二传手!10、“疏导有方”。
二、判断以下各题是否正确, 若正确, 用T表示, 不正确用F表示,并给出简要说明,每题1分,共5分1. Ans:True.The barriers formed by tight junction proteins restrict the flow ofmolecules between cells and the diffusion of proteins(and lipids)from the apical to the basolateral domain and vice versa.2. Ans:False.During transcytosis,vesicles that form from either the apical or basolateralsurface first fuse with early endosomes, then move to recycling endosomes,where they are sorted into transport vesicles bound for the opposite surface.3. Ans: True. All the core histones are rich in lysine and arginine, whichhave basic—positively charged—side chains that can neutralize thenegatively charged DNA backbone.4. Ans: True. When ATP in actin filaments (or GTP in microtubules) is hydrolyzed,much of the free energy released by cleavage of the high-energy bond is storedin the polymer lattice, making the free energy of the ADP-containing polymerhigher than that of the ATP-containing polymer. This shifts the equilibriumtoward depolymerization so that ADP-containing actin filaments disassemblemore readily than ATP-containing actin filaments.5. Ans: False. Although cyclin–Cdk complexes are indeed regulated byphosphorylation and dephosphorylation, they can also be regulatedby the binding of Cdk inhibitor (CKI) proteins. Moreover, the ratesof synthesis and proteolysis of the cyclin subunits are extremelyimportant for regulating Cdk activity.三、选择题(请将所选答案字母填入括号,简要说明选择理由,每题1分,共5 分)1、答:B2、答: B3、答: A4、答:B5、答:C四、简答题(每题5 分,共25分)1.Ans: The specificity for both the transport pathway and the transported cargo come notfrom the clathrin coat but from the adaptor proteins that link the clathrin to the transmembrane receptors for specific cargo proteins.The several varieties of adaptor proteins allow different cargo receptors,hence different cargo proteins,to be transported along specific transport pathways.Incidentally, humans are different from most other organisms in that they have two heavy-chain genes.Like other mammals,they also have two lightchain genes. In addition,in the neurons of mammals the light-chain transcripts are alternativelyspliced.Thus,there exists the potential in humans for additional complexity ofclathrin coats;the functional consequences of this potential variability are notclear.2.Ans: The building blocks—soluble subunits—of the three types of filaments are the basis for their polarity differences. The building blocks for actin filamentsB (an actinmonomer) and microtubules (ab-tubulin) have polarity—distinct ends—and thusform a polymer with distinct ends when they are linked together. By contrast, thebuilding block of intermediate filaments is a symmetric tetramer with identical ends.Thus, when these subunits are linked together, the ends of the resulting filament are also identical.3.Ans: Soluble ER proteins that are destined to reside in other membrane organelles or to be secreted are bound by transmembrane cargo receptors.The cytosolic domains ofthese cargo receptors bind to the COPII coats on the vesicles that form on the ERmembrane, incorporating the cargo receptors, along with their cargo, intoCOPII-coated vesicles.4.Ans: Because programmed cell death occurs on a large scale in both developing and adult tissues, it is important that it does not trigger the alarm reactions normallyassociated with cell injury. In tissue injury, for example, signals are released thatcan cause a destructive inflammatory reaction. Moreover, the release of intracellularcontents could elicit an immune response against molecules that are normally notencountered by the immune system. In normal development, such reactions would be self-defeating, even dangerous, if they occurred in response to programmed cell death.5、答:不能,如①核定位信号是永久性信号,需要反复使用,而通过ER的信号肽通常是临时性信号,如果核定位信号被切除,不利于有丝分裂后核的重建。
果蝇体内SOD和MDA随增龄变化及其与寿命的关系_张欣文

果蝇体内SOD和MDA随增龄变化及其与寿命的关系上海铁道大学医学院预防医学教研室(200070)张欣文徐思红厉曙光提要每日观察记录果蝇生存数和死亡数;将不同天龄雌雄果蝇分别制成匀浆,测定匀浆中SOD活力和M DA含量。
结果显示果蝇体内SO D活力在40、50天龄以前是随增龄而上升,进入老龄后开始下降;M DA含量随增龄而增加;各天龄雄蝇的SOD均高于雌蝇(P<0101);40天龄后雄蝇的存活率明显低于雌蝇(P<0.05或P<0101)。
表明果蝇体内SOD活力与M DA 含量的平衡失调可能是引起衰老和死亡的原因之一。
关键词黑腹果蝇SO D M DA寿命Eff ect of Age-dependent Variation of Superoxide Dismutse Activ-ity and Malonaldehyde Contents on Lif e-span in Drosophila Zhang X inwen,et al.D ep t.of Pr eventive M edicine,M edical College,Shanghai T iedao Univer sity(Shanghai200070) T o study the age-dependent var iation of sup er ox ide dis-mutse(SOD)activity and malonaldehyde(MDA)contents in Dr osop hila melanogaster and the corr elationship between the v ar i-ation and lif e-sp an.T he number s of liv ing and died f lies w er e recor ded daily.Flies of diff er ent ages(days)w er e homogenated sep arately.T he ex amining the SOD activ ity and M DA contents in the homogenates have been made.A ges of D rosop hila being y ounger than40、50days,the S OD activ ity increased with ad-vancing age,w hile that decr eased in aged Dr osop hila.T he levels of M DA incr eased signif icantly w ith a ging.T he S OD activity of male f lies is signif icant higher than that of f emale f lies in ev-ery group.T he p er cent sur v iv al of male f lies is signif icant low er than that of f emale f lies af ter40days.T he unbalance betw een SOD and MD A could be one of the f actor s causing aging and death.Key words Dr osophila melanogaster Superox ide dismutse Malonaldebyde Life-span果蝇虽属昆虫,但其许多代谢途径、生理学功能和发育阶段同哺乳动物基本相似,故常被用于衰老实验研究。
微卫星单碱基的英语

微卫星单碱基的英语英文回答:Microsatellites are short, repetitive sequences of DNA that are composed of a single nucleotide, such as A, C, G, or T. These sequences are found throughout the genome and are often located in non-coding regions. Microsatellites are highly polymorphic, meaning that they vary in length between individuals. This polymorphism makes them usefulfor a variety of genetic applications, such as population genetics, forensic science, and paternity testing.The most common type of microsatellite is the dinucleotide repeat, which consists of two nucleotides repeated in tandem. Dinucleotide repeats are typically named after the two nucleotides that they are composed of, such as AC, AG, or AT. Other types of microsatellites include trinucleotide repeats, tetranucleotide repeats, and pentanucleotide repeats.Microsatellites are thought to arise through a process called replication slippage. This process occurs when DNA polymerase pauses during replication and repeats the same nucleotide several times. Microsatellites can also be created or lengthened through a process called unequal crossing-over. This process occurs when two homologous chromosomes misalign during meiosis and exchange genetic material.Microsatellites are highly polymorphic because they are prone to mutations. These mutations can occur through a variety of mechanisms, including replication slippage, unequal crossing-over, and gene conversion. Microsatellite mutations can have a number of different effects, including changes in gene expression, changes in protein structure, and changes in disease susceptibility.Microsatellites are a valuable tool for a variety of genetic applications. They are used in population genetics to study the genetic diversity of populations and to track the flow of genes between populations. Microsatellites are also used in forensic science to identify individuals andto link suspects to crime scenes. Microsatellites are also used in paternity testing to determine the biologicalfather of a child.中文回答:微卫星是长度较短的重复性 DNA 序列,由单个核苷酸组成,如A、C、G 或 T。
常用酿酒酵母菌株基因型

常⽤酿酒酵母菌株基因型Commonly used strainsinformation include:used lab strainsidentity between common lab strainsS288CGenotype:MATαSUC2 gal2 mal mel flo1 flo8-1 hap1 ho bio1 bio6Notes: Strain used in the systematic sequencing project, the sequence stored in SGD. S288C does not form pseudohyphae. In addition, since it has a mutated copy of HAP1, it is not a good strain for mitochondrial studies. It has an allelic variant of MIP1 which increases petite frequency. S288C strains are gal2- and they do not use galactose anaerobically.The S288C genome was recently resequenced at the Sanger Institute.References:Mortimer and Johnston (1986) Genetics 113:35-43.BY4743Genotype:MAT a/αhis3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15 ura3Δ0/ura3Δ0Notes: Strain used in the systematic deletion project, generated from a cross between BY4741 and BY4742, which are derived from S288C. As S288c, these strains have an allelic variant of MIP1 which increases petite frequency. See Brachmann et al. reference for details.References:Brachmann et al. (1998) Yeast 14:115-32.FY4Genotype:MAT aNotes: Derived from S288C.References:Winston et al. (1995) Yeast 11:53-55.FY1679Genotype:MAT a/αura3-52/ura3-52 trp1Δ63/TRP1 leu2Δ1/LEU2 his3Δ200/HIS3 GAL2/GALNotes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.References:Winston et al. (1995) Yeast 11:53-55.AB972Genotype:MATα X2180-1B trp10 [rho 0]Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. AB972 is an ethidium bromide-induced rho- derivative of the strain X2180-1B-trp1.References:Olson MV et al. (1986) Proc. Natl. Acad. Sci. USA 83:7826-7830.A364AGenotype:MAT a ade1 ade2 ura1 his7 lys2 tyr1 gal1 SUC mal cup BIONotes: Used in the systematic sequencing project, the sequence stored in SGD.References:Hartwell (1967) J. Bacteriol. 93:1662-1670.XJ24-24aGenotype:MAT a ho HMa HMα ade6 arg4-17 trp1-1 tyr7-1 MAL2Notes: Derived from, but not isogenic to, S288CReferences:Strathern et al. (1979) Cell 18:309-319DC5Genotype:MAT a leu2-3,112 his3-11,15 can1-11Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.References:Broach et al. (1979) Gene 8:121-133X2180-1AGenotype:MAT a SUC2 mal mel gal2 CUP1Notes:S288c spontaneously diploidized to give rise to X2180. The haploid segregants X2180-1a and X2180-1b were obtained from sporulated X2180YNN216Genotype:MAT a/αura3-52/ura3-52 lys2-801amber/lys2-801amber ade2-101ochre/ade2-101ochreNotes: Congenic to S288C (see Sikorski and Hieter). Used to derive YSS and CY strains (see Sobel and Wolin). References:Sikorski RS and Hieter P (1989) Genetics 122:19-27.YPH499Genotype:MAT a ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1Notes: Contains nonrevertible (deletion) auxotrophic mutations that can be used for selection of vectors. Notethat trp1-Δ63, unlike trp1-Δ1, does not delete adjacent GAL3 UAS sequence and retains homology to TRP1 selectable marker.gal2-, does not use galactose anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.References:Sikorski RS and Hieter P (1989) Genetics 122:19-27.YPH500Genotype:MATαura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1Notes:MATα strain isogenic to YPH499 except at mating type locus. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C. References:Sikorski RS and Hieter P (1989) Genetics 122:19-27.YPH501Genotype:MAT a/MATαura3-52/ura3-52 lys2-801_amber/lys2-801_amber ade2-101_ochre/ade2-101_ochretrp1-Δ63/trp1-Δ63 his3-Δ200/his3-Δ200 leu2-Δ1/leu2-Δ1Notes:a/α diploid isogenic to YPH499 and YPH500. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.References:Sikorski RS and Hieter P (1989) Genetics 122:19-27.Sigma 1278BNotes: Used in pseudohyphal growth studies. Detailed notes about the sigma strains have been kindly provided by Cora Styles.Sigma1278B background contain a nonsense mutation in RIM15, a G-to-T transversion at position 1216 that converts a Gly codon to an opal stop codon. This rim15 mutation interacts epistatically with mutations in certain other genes to affect colony morphology.Annotation of the Sigma1278b genome and information about the systematic deletion collection can be found here. SK1 Genotype:MAT a/α HO gal2 cup S can1R BIONotes: Commonly used for studying sporulation or meiosis. Canavanine-resistant derivative.The SK1 genome was sequenced at the Sanger Institute.References:Kane SM and Roth J. (1974) Bacteriol. 118: 8-14CEN.PK (aka CEN.PK2)Genotype:MAT a/α ura3-52/ura3-52 trp1-289/trp1-289 leu2-3_112/leu2-3_112 his3 Δ1/his3 Δ1 MAL2-8C/MAL2-8CSUC2/SUC2Notes: CEN.PK possesses a mutation in CYR1 (A5627T corresponding to a K1876M substitution near the end of the catalytic domain in adenylate cyclase which eliminates glucose- and acidification-induced cAMP signalling and delaysReferences:van Dijken et al. (2000) Enzyme Microb Technol 26:706-714W303Genotype:MAT a/MATα {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15} [phi+]Notes: W303 also contains a bud4 mutation that causes haploids to bud with a mixture of axial and bipolar budding patterns. In addition, the original W303 strain contains the rad5-535 allele. As S288c, W303 has an allelic variantof MIP1 which increases petite frequency.The W303 genome was sequenced at the Sanger Institute.References: W303 constructed by Rodney Rothstein (see detailed notes from RR and Stephan Bartsch).bud4 info: Voth et al. (2005) Eukaryotic Cell, 4:1018-28.rad5-535 info: Fan et al. (1996) Genetics 142:749W303-1AGenotype:MAT a {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15}Notes: W303-1A possesses a ybp1-1 mutation (I7L, F328V, K343E, N571D) which abolishes Ybp1p function, increasing sensitivity to oxidative stress.References: W303 constructed by Rodney Rothstein (see detailed notes from RR and Stephan Bartsch).ybp1-1 info: Veal et al. (2003) J. Biol. Chem. 278:30896-904.W303-1BGenotype:MATα {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15}References: W303 constructed by Rodney Rothstein (see detailed notes from RR and Stephan Bartsch).W303-K6001Genotype:MAT a; {ade2-1, trp1-1, can1-100, leu2-3,112, his3-11,15, GAL, psi+, ho::HO::CDC6 (at HO), cdc6::hisG,ura3::URA3 GAL-ubiR-CDC6 (at URA3)}References: K6001 was developed by Bobola et al in Kim Nasmyth's lab (PMID: 8625408), and has become a common model in yeast aging research (PMID: 15489200). Its genome has been sequenced by Timmermann et al (PMID: 20729566) D273-10BGenotype:MATαmalNotes: Normal cytochrome content and respiration; low frequency of rho-. This strain and its auxotrophic derivatives were used in numerious laboratories for mitochondrial and related studies and for mutant screens. Good respirer that's relatively resistant to glucose repression.References:Sherman, F. (1963) Genetics 48:375-385.FL100Genotype:MAT aReferences:Lacroute, F. (1968) J. Bacteriol. 95:824-832.Sources: ATCC: 28383SEY6210/SEY6211Genotype:MAT a/MATαleu2-3,112/leu2-3,112 ura3-52/ura3-52 his3-Δ200/his3-Δ200 trp1-Δ901/trp1-Δ901ade2/ADE2 suc2-Δ9/suc2-Δ9 GAL/GAL LYS2/lys2-801Notes: SEY6210/SEY6211, also known as SEY6210.5, was constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers, good growth properties and good sporulation.References:Robinson et al. (1988) Mol Cell Biol 8(11):4936-48SEY6210Genotype:MATαleu2-3,112 ura3-52 his3-Δ200 trp1-Δ901 suc2-Δ9 lys2-801; GALNotes: SEY6210 is a MATalpha haploid constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers and good growth properties.References:Robinson et al. (1988) Mol Cell Biol 8(11):4936-48SEY6211Genotype:MAT a leu2-3,112 ura3-52 his3-Δ200 trp1-Δ901 ade2-101 suc2-Δ9; GALNotes: SEY6211 is a MATa haploid constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers and good growth properties.References:Robinson et al. (1988) Mol Cell Biol 8(11):4936-48JK9-3dThere are a, alpha and a/alpha diploids of JK9-3d with the following genotypes:Genotypes: JK9-3da MAT a leu2-3,112 ura3-52 rme1 trp1 his4JK9-3dα has the same genotype as JK9-3da with the exception of the MAT locusJK9-3da/α is homozygous for all markers except mating typeNotes: JK9-3d was constructed by Jeanette Kunz while in Mike Hall's lab. She made the original strain while Joe Heitman isolated isogenic strains of opposite mating type and derived the a/alpha isogenic diploid by mating type switching. It has in its background S288c, a strain from the Oshima lab, and a strain from the Herskowitz lab. It was chosen because of its robust growth and sporulation, as well as good growth on galactose (GAL+) (so that genes under control of the galactose promoter could be induced). It may also have a SUP mutation that allows translation through premature STOP codons and therefore produces functional alleles with many point mutations.88(5):1948-52RM11-1aGenotype:MAT a leu2Δ ura3Δ ho::KanNotes: RM11-1a is a haploid derivative of Bb32(3), a natural isolate collected by Robert Mortimer from a California vineyard, as in Mortimer et al., 1994. It has high spore viability (80–90%) and has been extensively characterized phenotypically under a wide range of conditions. It has a significantly longer life span than typical lab yeast strains and accumulates age-associated abnormalities at a lower rate. It displays approximately 0.5–1% sequence divergence relative to S288c. More information is available at the Broad Institute website.References:Brem et al. (2002) Science 296(5568):752-5Y55Genotype:MAT a /MAT alpha HO/HONotes: Y55 is a prototrophic, homothallic diploid strain that was originally isolated by Dennis Winge. Many auxotrophic mutant derivatives have been created by John McCusker by using ethidium bromide treatment to eliminate。
薛定谔—麦克斯韦尔方程径向解的存在性和多重性(英文)

In 1887, the German physicist Erwin Schrödinger proposed a radial solution to the Maxwell-Schrödinger equation. This equation describes the behavior of an electron in an atom and is used to calculate its energy levels. The radial solution was found to be valid for all values of angular momentum quantum number l, which means that it can describe any type of atomic orbital.The existence and multiplicity of this radial solution has been studied extensively since then. It has been shown that there are infinitely many solutions for each value of l, with each one corresponding to a different energy level. Furthermore, these solutions can be divided into two categories: bound states and scattering states. Bound states have negative energies and correspond to electrons that are trapped within the atom; scattering states have positive energies and correspond to electrons that escape from the atom after being excited by external radiation or collisions with other particles.The existence and multiplicity of these solutions is important because they provide insight into how atoms interact with their environment through electromagnetic radiation or collisions with other particles. They also help us understand why certain elements form molecules when combined together, as well as why some elements remain stable while others decay over time due to radioactive processes such as alpha decay or beta decay.。
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a r X i v :a s t r o -p h /0611844v 1 28 N o v 2006Astronomy &Astrophysics manuscript no.6383cESO 2008February 5,2008The millimetre variability of M81*Multi-epoch dual frequency mm-observations of the nucleus of M81R.Sch¨o del 1,M.Krips 2,S.Marko ff3,R.Neri 4,and A.Eckart 11I.Physikalisches Institut,Universit¨a t zu K¨o ln,Z¨u lpicher Str.77,D-50937K¨o ln,Germanye-mail:rainer@ph1.uni-koeln.de,eckart@ph1.uni-koeln.de2Harvard-Smithsonian Center for Astrophysics,SMA project,645North A’ohoku Place,Hilo,HI 96720e-mail:mkrips@3Sterrenkundig Instituut “Anton Pannekoek ”,Universiteit van Amsterdam,Kruislaan 403,1098SJ Amsterdam,The Netherlands e-mail:sera@science.uva.nl4Institut de Radio Astronomie Millim´e trique,300rue de la Piscine,Domaine Universitaire,38406Saint Martin d’H`e res,France e-mail:neri@iram.frReceived September ;acceptedABSTRACTAims.There are still many open questions as to the physical mechanisms at work in Low Luminosity AGN that accrete in the extreme sub-Eddington regime.Simultaneous multi-wavelength studies have been very successful in constraining the properties of Sgr A*,the extremely sub-Eddington black hole at the centre of our Milky Way.M81*,the nucleus of the nearby spiral galaxy M81,is an ideal source to extend the insights obtained on Sgr A*toward higher luminosity AGN.Here we present observations at 3and 1mm that were obtained within the framework of a coordinated,multi-wavelength campaign on M81*.Methods.The continuum emission from M81*was observed during three epochs with the IRAM Plateau de Bure Interferometer simultaneously at wavelengths of 3and 1mm.Results.We present the first flux measurements of M81*at wavelengths around 1mm.We find that M81*is a continuously variable source with the higher variability observed at the shorter wavelength.Also,the variability at 3and 1mm appears to be correlated.Like Sgr A*,M81*appears to display the strongest flux density and variability in the mm-to-submm regime.There remains still some ambiguity concerning the exact location of the turnover frequency from optically thick to optically thin emission.The observed variability time scales point to an upper size limit of the emitting region of the order 25Schwarzschild radii.Conclusions.The data show that M81*is indeed a system with very similar physical properties to Sgr A*and an ideal bridge toward high luminosity AGN.The data obtained clearly demonstrate the usefulness and,above all,the necessity of simultaneous multi-wavelength observations of LLAGN.Key words.LLAGN –mm-observations –galactic nucleus1.IntroductionAt high accretion rates onto black holes,the infall of gas and dissipation of energy via mostly thermal processes in a thin disk are fairly well understood in general terms.In con-trast,there is still great debate about which physical mech-anisms are dominant in objects which are accreting at well below the Eddington rate.The most extremely sub-Eddington source currently accessible to observations that allows still rea-sonable statistics for modelling the accretion /emission mech-anisms is the central supermassive black hole of our Galaxy,Sagittarius A*(Sgr A*).Its low luminosity has perplexed theo-rists for decades,and stimulated the re-emergence of radiatively ine fficient accretion flow models (RIAFS,see Melia &Falcke 2001;Quataert 2003),including the advection dominated ac-cretion flow model (ADAF,e.g.Narayan &Yi 1994)and its derivatives.These models are characterised by low e fficiency in converting thermal energy into electromagnetic radiation,but di ffer in terms of the inner boundary conditions such as the presence of strong winds (e.g.Blandford &Begelman 1999)and /or convection (Quataert &Gruzinov 1999),eachof2R.Sch¨o del et al.:The millimetre variability of M81* as contenders.Recently,Eckart et al.(2004,2006)were suc-cessful in obtaining thefirst simultaneous measurements of theemission from Sgr A*at X-ray/NIR wavelengths(with quasi-simultaneous sub-mm observations).These and ongoing coordi-nated multi-wavelengths campaigns deliver the decisive obser-vations for constraining/eliminating weak accretion models fur-ther.The nearby spiral galaxy M81(NGC3031)is an Sb spiralgalaxy similar to the Milky Way.It is located at a distance of3.63±0.34Mpc(Freedman et al.1994).Devereux et al.(2003)used spectroscopic measurements of the Hα+[N II]emission,probably emitted from a rotating gas disk inclined at an angle of14◦±2◦,to infer a mass of7.0+2−1×107M⊙for the central blackhole in M81.The nucleus of M81,termed M81*,shows typical signs of AGN activity.It has a power-law,variable X-ray continuum (Ishisaki et al.1996;Page et al.2004).The X-rayflux from M81 is highly variable,at scales from days to years(La Parola et al. 2004).The nucleus of M81displays double peaked,broad Hαemission lines(Bower et al.1996).However,the overall lumi-nosity and AGN characteristics of M81*are rather weak and the galaxy is classified as a LINER(low-ionisation nuclear emission-line region,e.g.Ho et al.1996).With a luminosity of the order of1037erg s−1in the radio and1040erg s−1in the optical/X-ray domains(see,e.g.,compilations by Ho et al.1996; Ho1999),its luminosity is<10−5times the Eddington luminos-ity in any wavelength regime.M81*is therefore counted among the low-luminosity AGN(LLAGN).It shows the typical spectral energy distribution(SED)of this class of sources,that is char-acterised by the absence of the so-called big blue bump,the ul-traviolet excess found commonly in the higher power AGN(e.g. Ho1999).At cm-wavelengths,M81*shows large-amplitude variations (factors up to two at2cm)with timescales of a few months and weaker changes of theflux density on timescales≤1day (Ho et al.1999).Multi-epoch VLBI observations of M81*at ∼0.01pc resolution at20epochs over4.5yr reveal a stationary core with a variable(on timescales of∼1yr)one-sided jet of length1mas(3600AU)towards the northeast(Bietenholz et al. 2000).As for the polarisation properties of M81*,circular po-larisation was detected at4.8,8.4(Brunthaler et al.2001),and 15GHz(Brunthaler et al.2006),while linear polarisation ap-pears to be absent at these frequencies.This is an intriguing sim-ilarity to Sgr A*,where circular polarisation also dominates over linear polarisation at these wavelengths(Bower et al.1999a,b,c; Aitken et al.2000;Bower et al.2003,2005).Sakamoto et al.(2001)present observations of the central kiloparsec of M81at a wavelength of3mm in the CO J=1−0 line and continuum at100pc resolution.They detect molecular gas in a pseudoring or spiral arm at about500pc,but no giant molecular cloud within about300pc of the nucleus.Theyfind significant intraday variation of the continuum emission from M81*,suggesting an emitting region of∼100AU.Reuter&Lesch(1996)obtained a spectrum of M81*from the radio to the mm-regime.Theyfind an inverted spectrum up to100GHz.Itsflux density can be described well by the law Sν∝ν1/3exp−(ν/νc)with a turnover frequency ofνc= 200GHz.They point out the remarkable similarity between the spectrum of M81*and Sgr A*and conclude that the same physi-cal mechanisms might operate in both galactic nuclei.Therefore Reuter&Lesch(1996)suggest that M81*may be a by∼104up-scaled version of Sgr A*.Thus M81*is a unique source for comparison with Sgr A* and more powerful AGN,and constitutes the next logical step after the successful multi-wavelength observations of Sgr A*. With this aim,a coordinated,multi-wavelength campaign took place in thefirst half of2005,involving instruments from the X-ray to the radio domain:the Chandra X-ray observatory,the Lick telescope(NIR),the SMA,the Plateau de Bure Interferometer (PdBI),the VLA,and the GMRT.A compilation of all observa-tions and the interpretation of the multi-wavelength data is pre-sented by Markoffet al.(in preparation).A detailed description of all instruments and the related data reduction would overload the multi-wavelength paper.Therefore,some papers are dedi-cated to the observations with specific instruments.The X-ray emission lines measured in the Chandra observations are dis-cussed by Young et al.(in preparation).In this paper we focus on the three epochs of mm-observations of M81*that were ob-tained with the PdBI during this campaign.2.Observations and data reductionM81*was observed with the PdBI on24February,14-15July, and19-20July2005.Six antennae were used for the February observations in the B configuration of the PdBI,which provides typical beam sizes around1.5′′at∼100GHz and around0.8′′at ∼200GHz.The July observations were done withfive antennae in the more compact D configuration,with typical beam sizes around5′′at∼100GHz and2.5′′at∼200GHz.All observations are listed in Table1.The observations aimed to detect continuum emission from M81*.However,due to good conditions the receivers were tuned to the12CO J=1−0and J=2−1transitions at115.3and 230.5GHz in the February observations.Thus,it was possible to search for compact CO-emission within∼20′′of the nucleus, while the continuum could be extracted from the line-free chan-nels(The results of the CO-line imaging will be discussed in a forthcoming paper).For greater phase stability in the July obser-vations,an epoch during which the atmosphere at the site con-tains more water vapour,the receivers were tuned to frequencies of80.5and86.2GHz at3mm and241.4and218.2GHz at1mm.The sources3C273(February),3C454.3(July14-15)and 1741-038(July19-20)were used for bandpass calibration. Phase calibration was performed with the sources1044+719and 0836+710.Primaryflux calibrators to determine the efficiencies of the antennae were the sources1044+719for February23-24(1.6Jy at3mm/1.1Jy at1mm),1044+719(1.8Jy at3mm) and2200+420(8.7Jy at1mm)for July14/15,and MWC349 (1.0Jy at3mm)and3C454.3(33.0Jy at1mm)for July20.The phase calibrators1044+719and0836+710were used tofit the time-dependentfluctuations of the amplitude for all baselines. Various tests were performed for estimating the uncertainty of theflux calibration,ing different primaryflux calibrators and comparing the resultingfluxes of all observed sources.As an additional test,from a comparison of the calibratedfluxes of the sources0836+710,1044+719,and MWC349between the three epochs we estimated the uncertainty of the absoluteflux calibra-tion.To provide a quantitative measurement of the data quality, Table2lists the ranges of the rms values of the phase and am-plitude that were obtained during calibration of these values for the different baselines for each observing epoch.The table also provides values for the estimated uncertainty of the absoluteflux calibration at3mm and1mm.As can be seen,the quality of the February data is highest.The data from20July are clearly better than the data from14/15July.Individual scans of20min duration were extracted from the calibrated data.Subsequently,a point source wasfitted to the re-sulting UV tables in order to determine theflux of M81*and ofR.Sch¨o del et al.:The millimetre variability of M81*3 Table1.Observations of M81*with the PdBI during2005.ν3mm is the exact frequency used around3mm,ν1mm the one at a wavelength of1mm.N Ant is the number of antennae,“Config”refers to the antenna configuration used.Start[UT]End[UT]ν3mm[GHz]ν1mm[GHz]N Ant ConfigTable2.Data quality:The table lists the ranges of the rms values of the phase and amplitude between all baselines for each observing epoch and wavelength.Also,the estimated systematic error of the absoluteflux calibration is indicated.3mm1mm1mmphase rms[deg]absolute absolute10-20≤10%≤15% 14/15-JUL-20058-1140-6025-3012-28≤15%≤20%4R.Sch¨o del et al.:The millimetre variability ofM81*Fig.1.Light curves of M81*and of the calibrators 1044+719and 0836+710from the PdBI observations at 3and 1mm.The x-axes show UT in hours.The fluxes were all scaled to an average value of 1,with the curves of the calibrators shifted for bettercomparison.The vertical lines in the top panels indicate sections for which the average flux and the standard deviation of the individual measurements have been calculated.The corresponding values are indicated.a sinusoidal pattern due to polarisation were observed at 3mm,one would expect to observe a similar pattern at 1mm,shifted by several hours.We do not detect the expected sinusoidal pattern in the light curves nor are the light patterns shifted by several hours in time relative to each other.b)Recent observations of M81*with the BIMA array by Brunthaler et al.(2006)indicateabsence of or very low upper limits of a few percent on linear and circular polarisation of M81*at 86and 230GHz.R.Sch¨o del et al.:The millimetre variability of M81*5 Fig.2.Light curves of M81*and of the calibrators1044+719and0836+710from the PdBI observations at3and1mm on February 24.The light curves were corrected for possible systematic variations by forcing the calibrator sources to have constantflux(onaverage).Table3.Averageflux densities of M81*,S3mm and S1mm,measured atν3mm andν1mm during the three epochs of the campaign. Themean and standard deviation(not the error of the mean)were calculated from the unweighted individual measurements shown in Fig.3.The last column lists the average spectral indices and their standard deviation(not the error of the mean)calculated from all individual measurements as shown in the right panels of Fig.4.Start[UT]End[UT]ν3mm[GHz]S3mmν1mm[GHz]S1mmαFig.3.Flux density of M81*on24February,14/15July,and20July2005.The black boxes mark theflux density at3mm and the green(gray)crosses mark theflux density at1mm.The exact frequencies are indicated in the titles of the individual panels.The error bars indicate relative,not absolute,uncertainties.4.DiscussionTheory and observations indicate that sub-Eddington black holesare jet-dominated(Falcke&Markoff2000;Falcke et al.2004;Yuan et al.2002;Fender et al.2003).A compact,variable jet hasin fact been detected in VLBI observations of M81*(Bietenholzet al.,2000;see also Markoffet al.,in preparation).Falcke(1996)demonstrate that a compact jet can explain the observedspectrum of M81*very well.In the jet model,the turnover fre-quency from aflat(or slightly inverted)radio spectrum to anoptically thin power-law occurs at a frequencyνt.In a simplifiedmodel this frequency depends on the jet power,Q j,and mass ofthe black hole,M BH,asνt∝Q2/3jM−1BH(Falcke et al.2004).InLLAGN the turnover occurs generally at(sub)mm wavelengths,while it is located in the near-infrared/optical regime for XRBsin the low/hard state(see Corbel&Fender2002;Markoffet al.2003;Falcke et al.2004).The simultaneous observations of M81*at3and1mm pre-sented in this work are consistent with a turnover of the syn-chrotron emission from a jet in M81*into the optically thinpart between3mm and1mm(with the exception of thefirst∼4hours of the24February light curve that would indicate ahigher turnover frequency).This is in good agreement with thedata presented by Reuter&Lesch(1996)that were,however,notacquired simultaneously.6R.Sch¨o del et al.:The millimetre variability ofM81*Fig.4.Spectral index of M81*on24February,14/15July,and20July2005.The spectral index is defined by Sν∝να,where Sνis theflux density at a given frequencyν.The absolute uncertainties(see Tab.2)of theflux densities at3and1mm have been taken into account for this plot.Since we use upper limits on the absolute uncertainties,the plotted error bars are conservative.The evidence for the location of the turnover frequency be-tween3and1mm is ambiguous,however.The multi-frequency radio data from the coordinated campaign indicate that bumps are present in the radio spectrum(Markoffet al.,in prep.).These bumps are located at different frequencies at different epochs. Therefore,in spite of the presented mm-data,it may well be pos-sible that there is a submm-bump present in M81*.Observations with the SMA at345Ghz that were obtained during the coordi-nated campaign on M81*indicate that theflux density of M81* increases toward the submm regime,in agreement with theo-retical predictions on the existence of a submm-bump(Markoffet al.,in preparation).Unfortunately,there was only one epoch (24February2005),where measurements with the PdBI and the SMA were simultaneous.Forfine-tuning the models of the emission of M81*it is im-portant to know the exact turnover frequency and whether and how it varies with activity of the source.Therefore there is an urgent need for more observational data for comparison with theory in order to understand sub-Eddington accretion and emis-sion.It is essential that the observations across the wavelength regimes are simultaneous.Due to the gap of several months there is probably no corre-lation between the February and July observations because the light curves at2cm presented by Ho et al.(1999)indicate that radio outbursts of M81*have generally timescales<5months. Also,Sakamoto et al.(2001)have found that M81*showsflux variations of factors≤2at a wavelength of3mm on time scales <10days.However,the decaying light curve from July19may be related to the same event as the light curve on July14.The spectral index during the two epochs is negative and of similar absolute value(see Tab.3and Fig.4).This behaviour is con-sistent with a decaying light curve from a low-peakingflare in the model by Valtaoja et al.(1992)that describes variability phe-nomena in AGN by a growth/decay of shocks in a jet(see also Marscher&Gear1985).An estimate of the size scales of the relevant processes can be obtained from the time scales of the observed variability.The drop-offbetween07:00UT and12:00UT in the highest quality data set from24February2006shows that a 5σvariability of theflux at3mm occurs on a time scale of5hours(see also the intraday variability at3mm found by Sakamoto et al.2001).A similarly rapid change of theflux can be seen at1mm.Since no signal can travel faster than at the speed of light this cor-responds to an upper limit on the size of the source of merely ∼25Schwarzschild radii,when a mass of7.0×107M⊙is as-sumed for the black hole in M81*.5.SummaryWe present three epochs of simultaneous1and3mm contin-uum observations of the LLAGN M81*that were obtained in the framework of a coordinated,multi-wavelength campaign (Markoffet al.,in preparation).The observations of M81*with the PdBI at mm-wavelengths confirm that M81*is a continuously varying radio contin-uum source as has been found previously,mostly at larger wavelengths(see Ho et al.1999;Bietenholz et al.2000; Sakamoto et al.2001).The measurements present thefirst un-ambiguous detection of M81*at1mm and moreover show that the source is continuously variable at this wavelength as well. The amplitude of the variability is observed to be generally larger at1mm than at3mm by a factor of roughly1.5.This agrees well with the trend found by Ho et al.(1999)that the am-plitude of the variability increases with frequency in LLAGN.A similar behaviour is found in the far weaker source Sgr A*(e.g. Herrnstein et al.2004;Miyazaki et al.2004).The shortest variability time scales of our observations give upper limits on the size of the emitting region of ∼25Schwarzschild radii,assuming a black hole mass of7.0×107M⊙.The decaying light curves observed on14/15and on20July 2005may have been related to the same radio outburst.They are consistent with a generalized shock-in jet model(Valtaoja et al. 1992).The simultaneous measurements of M81*at3and1mm are consistent with a turnover of theflux in the mm-to-submm regime as predicted by models for a jet dominated source.Some ambiguity remains concerning the exact peak frequency and the related interpretation of variability events.The observations confirm previousfindings that there are many similarities between M81*and Sgr A*,the source re-lated to the supermassive black hole at the center of the Milky Way.This underlines the similarity between LLAGN despite of several orders of magnitudes of difference between their lumi-nosities.M81*can serve as a bridge from the extremely sub-Eddington Sgr A*toward higher luminosity LLAGN.The radio emission from M81*is apparently dominated by a compact jet. 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