Wistar大鼠C6胶质瘤脑移植模型的应用

Wistar大鼠C6胶质瘤脑移植模型的应用
易林华;傅相平;李安民;闫润民
【期刊名称】《南方医科大学学报》
【年(卷),期】2011(31)2
【摘要】Objective To establish a rat model bearing brain glioma and investigate the optimal conditions for its experimental application. Methods C6 cells were implanted into the unilateral brain hemisphere of 20 Wistar rats. The growth behaviors of the brain tumor and behavioral changes of the rats were observed at different time points after the implantation. Results On day 3 after the implantation, only a slight increase of signal intensity was observed on T2-weighted images. By day 5, the tumor became visible in 15/18 of the rats in at least two sections. By day 11,16/ 18 of the rats showed space-occupying effect in the brain, and by day 14, the tumor occupied over 1/2 of the hemisphere in 14/18 of the rats. By day 20, 14/18 of the rats showed a tumor mass occupying over 2/3 of the hemisphere, and some tumor cells had migrated into the contralateral hemisphere. Conclusion In this model of brain glioma, the optimal time widow for experiment is between 14 and 18 days after the cell implantation. The cell density and viability for implantation and the site of implantation may also affect the experimental time widow.%目的通过观察C6胶质瘤大鼠脑移植模型,了解该模型的牛物学特点,以帮助实验中有效应用.方法Wistar大鼠20只,将活性较好的C6胶质瘤细胞微量移植人大鼠大脑半球,观察肿
瘤生长特点及行为学改变.结果肿瘤移植后第3天,TW2即可出现轻微高信号改变.15/18的大鼠在第5天至少能看到2个层面的信号改变.16/18的大鼠在第11天能看见实质性肿瘤.13/18的大鼠第14天生长至半侧大脑的1/2.14/18的大鼠第20大大部分肿瘤达到半侧大脑的2/3,部分生长至对侧半球.结论实验可能的最佳时间窗在14～18 d.同时,肿瘤移植的初始细胞浓度、细胞活性和移植部位可能也对实验时间窗会有较大影响.
【总页数】3页(P277-279)
【作者】易林华;傅相平;李安民;闫润民
【作者单位】解放军总医院第一附属医院神经外科,北京,100048;解放军总医院第一附属医院神经外科,北京,100048;解放军总医院第一附属医院神经外科,北
京,100048;解放军总医院第一附属医院神经外科,北京,100048
【正文语种】中文
【中图分类】R73-352
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