dsc_18-6-1_-_outcome_of_mepc_64_and_fp_56_(secretariat)
Autodesk Nastran 2023 参考手册说明书

FILESPEC ............................................................................................................................................................ 13
DISPFILE ............................................................................................................................................................. 11
File Management Directives – Output File Specifications: .............................................................................. 5
BULKDATAFILE .................................................................................................................................................... 7
气泡混合轻质土使用规程

目次1总则 (3)2术语和符号 (4)2.1 术语 (4)2.2 符号 (5)3材料及性能 (6)3.1 原材料 (6)3.2 性能 (6)4设计 (8)4.1 一般规定 (8)4.2 性能设计 (8)4.3 结构设计 (9)4.4 附属工程设计 (10)4.5 设计计算 (10)5配合比 (13)5.1 一般规定 (13)5.2 配合比计算 (13)5.3 配合比试配 (14)5.4 配合比调整 (14)6工程施工 (15)6.1 浇筑准备 (15)6.2 浇筑 (15)6.3 附属工程施工 (15)6.4 养护 (16)7质量检验与验收 (17)7.1 一般规定 (17)7.2 质量检验 (17)7.3 质量验收 (18)附录A 发泡剂性能试验 (20)附录B 湿容重试验 (22)附录C 适应性试验 (22)附录D 流动度试验 (24)附录E 干容重、饱水容重试验 (25)附录F 抗压强度、饱水抗压强度试验 (27)附录G 工程质量检验验收用表 (28)本规程用词说明 (35)引用标准名录 (36)条文说明 (37)Contents1.General provisions (3)2.Terms and symbols (4)2.1 Terms (4)2.2 Symbols (5)3. Materials and properties (6)3.1 Materials (6)3.2 properties (6)4. Design (8)4.1 General provisions (8)4.2 Performance design (8)4.3 Structure design (9)4.4 Subsidiary engineering design (9)4.5 Design calculation (10)5. Mix proportion (13)5.1 General provisions (13)5.2 Mix proportion calculation (13)5.3 Mix proportion trial mix (14)5.4 Mix proportion adjustment (14)6. Engineering construction (15)6.1 Construction preparation (15)6.2 Pouring .............................................................. .. (15)6.3 Subsidiary engineering construction (16)6.4 Maintenance (17)7 Quality inspection and acceptance (18)7.1 General provisions (18)7.2 Quality evaluate (18)7.3 Quality acceptance (19)Appendix A Test of foaming agent performance (20)Appendix B Wet density test (22)Appendix C Adaptability test (23)Appendix D Flow value test.................................................................................. .. (24)Appendix E Air-dry density and saturated density test (25)Appendix F Compressive strength and saturated compressive strength test (27)Appendix G Table of evaluate and acceptance for quality (28)Explanation of Wording in this code (35)Normative standard (36)Descriptive provision (37)1总则1.0.1为规范气泡混合轻质土的设计、施工,统一质量检验标准,保证气泡混合轻质土填筑工程安全适用、技术先进、经济合理,制订本规程。
衰减全反射中红外光谱测定马铃薯中可溶性蛋白含量

衰减全反射中红外光谱测定马铃薯中可溶性蛋白含量陈美林;陈业;张玉婷;宋波涛;莫开菊【期刊名称】《中国粮油学报》【年(卷),期】2018(033)012【摘要】傅里叶变换衰减全反射红外光谱法是一种快速无损绿色的分析技术,结合化学计量学和数学建模可对混合物进行定性、定量分析.本研究利用衰减全反射中红外光谱建立测定马铃薯中可溶性蛋白含量的方法,以满足选育高蛋白质含量马铃薯的快速测定,提高快速鉴定育种品系品质的目的.采用平滑降噪和导数基线矫正的方法预处理数据,选择1700~ 600 cm-1波段作特征光谱,提取主成分并逐步回归建模.结果表明7点平滑后一阶导数处理数据,能较准确地测定马铃薯中可溶性蛋白的含量,对于验证集的预测,其中70.37%预测值偏差小于20%,对于鲜重蛋白质含量在5 mg/g以上的14个样品的预测,高达92.86%预测值偏差小于20%,说明能准确快速鉴定高蛋白品系.【总页数】9页(P118-126)【作者】陈美林;陈业;张玉婷;宋波涛;莫开菊【作者单位】湖北民族学院生物科学与技术学院,恩施 445000;湖北民族学院生物科学与技术学院,恩施 445000;华中农业大学园艺林学学院;农业部马铃薯生物学与生物技术重点实验室,武汉 430070;华中农业大学园艺林学学院;农业部马铃薯生物学与生物技术重点实验室,武汉 430070;湖北民族学院生物科学与技术学院,恩施445000【正文语种】中文【中图分类】O657.33【相关文献】1.基于中红外衰减全反射光谱的氮同位素标记硝态氮的快速测定 [J], 邵艳秋;杜昌文;申亚珍;马菲;周健民2.应用拉曼和中红外衰减全反射光谱测定溶液和土壤中的硝酸盐 [J], 邵艳秋;杜昌文;申亚珍;马菲;周健民3.衰减全反射-中红外光谱法定量测定小麦粉中的石灰类添加物 [J], 王冬;王纪华;马智宏;潘立刚;韩平;赵柳4.衰减全反射中红外光谱法快速测定车用汽油中共轭二烯烃和烯烃含量 [J], 吕玉平;陈鸿雁;闻环;王荣华;林志生;徐玲5.衰减全反射中红外光谱法快速测定柴油十六烷值和十六烷指数 [J], 吕玉平;程秀;闻环;徐玲;李荣健;邓小媞因版权原因,仅展示原文概要,查看原文内容请购买。
过表达及敲除细胞系建立及所需材料详细

293T
NC shRNA1 2
3
4
5
X actin
72kd
293T
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+
Flag-X
Flag-X actin
Step3: stable transfection and verification: 6-10days
1.利用浓度梯度法寻找最适筛选浓度:将待筛选细胞按照60%-80%左右融合度 种于六孔板中,设置筛选药物如下浓度:0,0.25, 0.5, 0.75, 1, 2µg/ml,加入 已贴壁细胞中,每24小时观察细胞活率,3-5天内细胞全部死亡的最低浓度为最 适浓度。 2.包装病毒步骤: (1)第一天种293T于六孔板中,每孔约60%-80%融合度; (2)利用gag-pol、vsv-g包装质粒,用Xtreme-gene9转入细胞中,37度细 胞培养箱培育,24小时后换液,48小时收病毒上清; (3)收病毒前一天种待感染细胞于六孔板中,每孔60%-80%融合度; (4)用0.45µm的滤头过滤病毒上清后,将病毒上清加入待感染细胞中,加入 polybrene使其终浓度为8µg/ml,1500g,22-27度,90min离心; (5)离心后加入2.5ml培液稀释polybrene,放入37度培养箱培养; (6)48小时后,加入最适浓度抗生素药筛5-7天,同时设置对照组参考。
2 ×SDS(甘油,DTT,Tris 6.8,SDS配置);Bradford(康为);溴酚蓝(ameresico);电 泳液(甘氨酸、SDS、Tris配置10 × ,使用时稀释成1 × );转膜液(甘氨酸、Tris配置10 × , 使用时稀释成1 × 并加入甲醇) marker(thermo);antibody(abcam、CST、MBL、 Sigma);SDS(sigma)PAGE、Tris6.8、8.8(生工);APS、TEMED(ameresico);0.22 及0.45µmPVDF膜(minipore)
基于QuEChERS_样品前处理的间接竞争酶联免疫分析法快速检测薏苡仁中黄曲霉毒素B1

广东药科大学学报Journal of Guangdong Pharmaceutical University Nov,2023,39(6)基于QuEChERS 样品前处理的间接竞争酶联免疫分析法快速检测薏苡仁中黄曲霉毒素B 1张磊1,2,3,关凯仪1,2,3,黄雨心1,2,3,王淑美1,2,3(1.广东药科大学中药学院,广东广州510006;2.国家中医药管理局中药数字化质量评价技术重点研究室,广东广州510006;3.广东省中药质量工程技术研究中心,广东广州510006)摘要:目的解决间接竞争酶联免疫分析法(indirect competitive enzyme-linked immunosorbent assay ,ic-ELISA )检测薏苡仁中黄曲霉毒素B 1(Aflatoxin B 1,AFB 1)的基质干扰问题,建立一种简便的高通量检测方法,用于薏苡仁中AFB 1的快速筛查。
方法以ic-ELISA 的显色值和抑制率为评价指标优化样品前处理过程,重点考察QuEChERS 萃取净化技术、不同稀释溶剂和稀释方式消除基质效应的效果,并对建立的方法进行方法学考察,最后应用于薏苡仁实际样品检测,检测结果用液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry ,LC-MS/MS )进行确证。
结果建立了一种无需任何校正,可用溶剂标准曲线进行定量分析的ic-ELISA 。
方法的半数抑制浓度为0.074ng/mL ,线性范围为1.37~20.0μg/kg ,加标回收率在75.12%~84.46%之间,RSD 小于6%。
122批薏苡仁及其相关样品中AFB 1的阳性率为20.5%,阳性样品的超标率为24.0%,污染水平为1.76~27.56μg/kg 。
ic-ELISA 与LC-MS/MS 检测结果的相关系数(r )为0.98。
结论QuEChERS 萃取净化技术能够有效去除薏苡仁中的干扰成分,对快速样品前处理方法的建立具有重要应用价值,结合本研究所建立的ic-ELISA ,为薏苡仁中AFB 1的检测和监测提供简便有效的技术手段。
Freescale - MC56F8006DEMO-T-PROMO 数字信号控制器(DSC)评估、演

Freescale - MC56F8006DEMO-T-PROMO -MC56F8006DEMO-T-PROMO Demo KitProduct Overview:MC56F8006DEMO-T-PROMO is a cost-effectiveboard targeting quick digital signal controller (DSC)evaluation, demonstration and debugging of theFreescale MC56F8006VLF digital signal controller.The board is equipped to handle USB communicationright out of the box. MC56F8006 demo board has aseries of 6 leds connected to PWM channels in orderto monitor signals and has a Reset and 2 IRQswitches.Kit Contents:The MC56F8006DEMO-TOPROMO contains the hardware essentials you will need to use it. The items in the Development Kit and their use are as follows.∙DVD Getting Started With MC56F8006/2Free CodeWarrior Development Studio for 56F800/E DSC, Special Edition (32KB code size limit)Device and Board Resources7 different MC56F8006 Demo Labs including LED flashing demonstration, USB Serial , Freemaster and FIR Labs.TAP∙ USBCable∙ USB∙MC56F8006DEMO-T Quick Start GuideCard∙ FreescaleWarrantyKey Features:∙Complete pin-out available including a 40 pin header compatible with all 56F80xx boards∙ Supply voltage options from USB connector, direct power supply in J1 and using standard power jack∙ MC56F8006 Demo board with USB connectivity ∙ JTAG control and debug of MC56F8006 ∙ BDM control and debug of MC9S08JM60 ∙ Serial Communications port ready for RS-232 ∙6 LEDs, connected to PWM channelsOrdering Information: Products:Part NumberManufacturerFarnell P/N Newark P/NMC56F8006DEMO-T-PROMO Freescale1758255NAAssociated Products:Part Number Manufacturer DescriptionFarnell P/N Newark P/N MC56F8006VLFFreescaleDigital Signal Controller IC171835811R0875 MC9S08JM60CGT Freescale MCU, 8-BIT, S08, 60KFLASH, QFN48 156286494M8314 USBMLCF FreescaleUSB ColdFire Multilink 1535884 01M6486 MAX3232CDBE4 TI True RS-232 Transceivers 1287435 26M0603 SN74LVC125ADTIQuad buffer driver110297594B6303 MIC2026-1BM MICREL Dual-Channel Power Distribution Switch 446196405C4401 LM1117DT-ADJ/NOPB NS Linear Voltage Regulator IC 1364485 41K3426 MC56F8006VLF Freescale Digital Signal Controller IC 1718358 11R0875 MC9S08JM60CLDFreescale8-Bit Microcontroller IC156286454M7190Similar Products:Part NumberManufacturerDescriptionSupport DeviceFarnell P/NNewark P/NMC56F8006DEMO FreescaleMC56F8006DSC Board MC56F8006 1748988 40P5046MC56F8006DEMO-T FreescaleMC56F8006DSC BoardMC56F8006 1748989 26R6765Document List:Datasheets:Part Number Description SizeMAX3232 3.0V to 5.5V, Low-Power, up to 1Mbps, True RS-232 Transceivers 272KB74LVC125 Quad buffer/line driver with 5 V tolerant input/outputs; 3-state 108KBMIC2026 Dual-Channel Power Distribution Switch 180KBLM1117 LM1117 800mA Low-Dropout Linear Regulator 532KBMC56F8006 MC56F8006 DSC Datasheet 1.82MBMC9S08JM60 MC9S08JM60 Datasheet 10.6MBMC56F8006 MC56F8006 Reference Manual 4MBApplication Notes:File Name SizeImplementing a Modular High Brightness RGB LED Network 1.82MBMC56F8006DEMO User Guide 3.19MBSingle Phase Two-Channel Interleaved PFC Converter Using MC56F8006 344KBHardware & Software:File Name SizeMC56F8006DEMO_FFT_lab 716KBMC56F8006DEMO_FIR_lab 2.17MBMC56F8006DEMO_Freemaster_lab 2.29MBMC56F8006DEMO_hall_motor_lab 796KBMC56F8006DEMO_LED_lab 232KBMC56F8006DEMO_PGA_lab 1MBMC56F8006DEMO_serial_lab 3.46MBMC56F8006DEMOLAB 64KB。
敲低MKL1通过激活自噬流抑制心肌成纤维细胞活化与纤维化表型的作用研究

敲低MKL1通过激活自噬流抑制心肌成纤维细胞活化与纤维化表型的作用研究魏俊萍;符达佳;孟庆雯;林道飞;林燕仔【期刊名称】《转化医学杂志》【年(卷),期】2024(13)1【摘要】目的探究敲低巨核细胞白血病因子1(MKL1)对心肌成纤维细胞(CFs)活化与纤维化表型的影响及作用机制。
方法选取人CFs,敲低CFs中MKL1表达,检测敲低效果;将CFs分为对照组、血管紧张素Ⅱ(AngⅡ)组、shNC+AngⅡ组、shMKL1+AngⅡ组,shNC+AngⅡ组与shMKL1+AngⅡ组分别转染shRNA NC、shRNA MKL1,AngⅡ组、shNC+AngⅡ组及shMKL1+AngⅡ组CFs再使用AngⅡ刺激建立心肌纤维化模型;处理结束后,检测各组CFs增殖活性,测定各组CFs 细胞培养上清液中Ⅰ型胶原(COL-Ⅰ)、Ⅲ型胶原(COL-Ⅲ)水平,观察各组CFs中α-平滑肌肌动蛋白(α-SMA)表达,检测各组CFs中α-SMA、COL-Ⅰ、COL-Ⅲ蛋白表达水平。
观察各组CFs自噬流,检测各组CFs中LC3Ⅱ/LC3Ⅰ蛋白比值、Beclin1蛋白表达水平。
结果转染shMKL1的CFs中MKL1 mRNA相对表达量与蛋白相对表达量均低于未转染的CFs和转染shNC的CFs(P<0.05);与对照组比较,AngⅡ组同一时间下CFs增殖活性升高(P<0.05),上清液中COL-Ⅰ、COL-Ⅲ水平升高(P<0.05),CFs中α-SMA荧光染色强度增加,α-SMA、COL-Ⅰ、COL-Ⅲ蛋白相对表达量上调(P<0.05),自噬溶酶体与自噬体减少,LC3Ⅱ/LC3Ⅰ蛋白比值下降(P<0.05),Beclin1蛋白相对表达量下调(P<0.05);与AngⅡ组、shNC+AngⅡ组比较,shMKL1+AngⅡ组同一时间下CFs增殖活性降低(P<0.05),上清液中COL-Ⅰ、COL-Ⅲ降低(P<0.05),CFs中α-SMA荧光染色强度减弱,α-SMA、COL-Ⅰ、COL-Ⅲ蛋白相对表达量下调(P<0.05),CFs中自噬溶酶体与自噬体增加,LC3Ⅱ/LC3Ⅰ蛋白比值升高(P<0.05),Beclin1蛋白相对表达量上调(P<0.05)。
胰岛素样生长因子-1对大鼠心肌缺血再灌注损伤的保护作用研究

胰岛素样生长因子-1 对大鼠心肌缺血再灌注损伤的保护作用研究发布时间:2021-08-30T05:58:45.110Z 来源:《医师在线》2021年21期作者:林梦飞[导读] 目的:探索胰岛素样生长因子-1 对大鼠心肌缺血再灌注损伤模型的保护作用林梦飞茂名市人民医院心血管内一科 525000【摘要】目的:探索胰岛素样生长因子-1 对大鼠心肌缺血再灌注损伤模型的保护作用。
方法:采用结扎冠脉前降支30 分钟后再予以灌注的方法建立雄性SD 大鼠心肌缺血/再灌注损伤模型。
建模成功后24 只大鼠模型随机平均分为IGF-1 1d 组、IGF-1 3d 组、PBS(安慰剂) 1d 组、PBS 3d 组4 个组,每组6 只,分别予安慰剂PBS 和IGF-1 处理1d 及3d 后观察以下指标:1.用DCFDA 荧光探针流式检测各组心肌细胞活性氧簇(Reactive oxygen species,ROS)水平;2.用TUNEL 染色法观察各组心肌细胞凋亡数;用流式检测各组心肌细胞凋亡率。
结果:1.IGF-1 1d 组与PBS 1d 组相比心肌ROS 阳性细胞比例、心肌细胞ROS平均荧光强度减少;2. IGF-1 3d 组与PBS 3d 组相比心肌细胞凋亡减少。
结论:IGF-1 可能通过减少缺血再灌注损伤大鼠模型心肌细胞氧自由基产生,减少心肌缺血再灌注损伤大鼠模型心肌细胞凋亡,对其有保护作用。
【关键词】IGF-1;大鼠心肌缺血再灌注损伤;病理损伤;细胞凋亡;氧自由基随着冠心病诊疗技术进步而提高的心肌缺血区血运再通率所带来次联级危害激活之一──心肌缺血再灌注损伤,作为临床上急性心梗溶栓疗法、外科冠脉搭桥术及经皮穿刺冠状动脉腔内血管成形术后等心律失常和心功能不全的病理基础,严重影响了冠心病患者的预后[1,2]。
因此积极寻找保护、改善心肌缺血再灌注损伤的策略,能进一步提高冠心病的防治水平、改善患者的预后。
探索IGF-1对缺血再灌注损伤大鼠模型的保护作用,可能为寻找心肌缺血再灌注损伤防治策略的提供未来新方向。
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I:\DSC\18\6-1.doc
E
SUB-COMMITTEE ON DANGEROUS GOODS, SOLID CARGOES AND CONTAINERS
18th session
Agenda item 6
DSC 18/6/1
7 March 2013 Original: ENGLISH
DEVELOPMENT OF AMENDMENTS TO THE IMSBC CODE AND SUPPLEMENTS, INCLUDING EVALUATION OF PROPERTIES OF SOLID BULK CARGOES
Outcome of MEPC 64 and FP 56
Note by the Secretariat
I MPLEMENTATION OF REVISED MARPOL A NNEX V
1 The Sub-Committee, at its seventeenth session, with regard to the implementation of the revised MARPOL Annex V, considered the views of the E&T 17 (DSC 17/4/2) that the development of a new section for environmentally hazardous substances is necessary within the IMSBC Code, together with document DSC 17/4/9 (Canada), suggesting that including a list of solid bulk materials in the Code, which fall under the revised MARPOL Annex V, could be helpful and expressing their concern over the difficulty of implementing the revision of MARPOL Annex V. Having noted that MEPC 64 would consider the above matters in relation to the revised MARPOL Annex V, DSC 17 agreed, in general, to have a new section in the future IMSBC Code relating to the revised MARPOL Annex V.
2 MEPC 64 instructed the Sub-Committee to consider how the long-term implementation of the provisions of MARPOL Annex V, concerning cargo residues, could be facilitated by amendments to the IMSBC Code.
DSC 18/6/1
Page 2
3 In this context, MEPC 64, having considered the report of the drafting group (MEPC 64/WP.13), agreed to the provisional classification of solid bulk cargoes under the revised MARPOL Annex V (MEPC.1/Circ.791).
F IRE EXTINGUISHIN
G ARRANGEMENTS IN CARGO SPACES (IMSBC C ODE)
4 FP 56 noted that DSC 17 had forwarded a draft interpretation to the IMSBC Code on fire-extinguishing arrangements in cargo spaces (DSC 17/WP.5, annex 6) to FP 56 for its expert consideration and advice to DSC 18.
5 In this regard, FP 5
6 noted that DSC 1
7 had deleted the last sentence of the IACS Unified Interpretation SC 250 (DSC 17/4/1/Rev.1, annex), which was used as the basis for the draft interpretation agreed at the session, on fixed gas fire-extinguishing systems or inert gas systems dedicated for the protection of spaces other than cargo spaces that should not be used for the purposes of preventing the consequences of self-heating of certain solid bulk cargoes.
6 In this connection, FP 56 concluded that the addition of the word "exclusively" after "dedicated" in the last sentence of IACS Unified Interpretation SC 250, would exclude from the text of the interpretation those fixed gas systems which are intended to protect just one space (e.g. engine-room), thus removing any ambiguities, and agreed that the sentence should be reinstated, as modified, in the text of the interpretation prepared at DSC 17. The FP Sub-Committee invited DSC 18 to consider the above view and take action accordingly.
A CTION REQUESTED OF THE S UB-C OMMITTEE
7 The Sub-Committee is invited to note the information provided and take action, as appropriate.
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