sciencell1001说明书
三洋科研医疗仪器产品说明书
血液保存箱
培养系统
MDF-U5411 MPR-214F / 414F / 414FS MPR-514 / 514R / 1014 / 1014R MPR-721 / 721R / 1410 / 1410R MBR-107D(H) / 506D(H) / 304D / 304DR / 304G / 304GR MBR-704G / 704GR / 1404G / 1404GR
家用空气净化器 ABC-VW24
商用空气净化器 VW-VF10BGC
电)。即买即用，而且充电完成后，即使在一年之后想用 时也能立即使用。
◎ 数码相机拍摄相片数量为干电池的4.4倍 eneloop比碱性干电池的电量更为持久。用数码相机拍摄 时，相片数量可达碱性干电池的4.4倍。除此之外，还可 用于遥控器和游戏机等，家庭中的各类使用电池的机器， 还可作为发生灾害时的备用电池，用途非常广泛。
◎ 抑制自然放电，即买即用 普通充电电池随着使用时间的变长，能量会逐渐减少， eneloop则大幅改善了一般充电电池的这一性质(自然放
印 该系列净化系统采用日常生活中的自来水配合三洋独
有的电解水技术实现洁净空气的目的。该系列产品针 对不同用户需求，开发商用及民用的专属型号，可为
司 日常生活提供安全与清新的空气。 公
2004
2005 2006
2007 2008
三洋医疗欧洲分部成立
技 术 特 点 提出GAIA概念
并入三洋能源与生态生物医疗部分,提出GAIA概念
贸 推出Cool Safe 压缩机技术
推出-150℃超低温保存箱MDF-C2156VAN -86℃超低温保存箱MDF-U53V/73V
国际 V.I.P. PLUS新技术的导入，推出全新V.I.P. PLUS技术的MDF-C8V(N)
EcoBlue 生物显微镜产品说明书
EC.1001MONOCULAR MODEL EC.1001355 (h) x 170 (w) x 220 (d) | 2.48 kg BINOCULAR MODEL EC.1152onocular, binocular and trinocular models with WF10x/18 mm he eyepieces of the binocular and trinocular models are suppliedI L L U M I N AT I O N• M onocular models are supplied with a 1 W LED illumination with integrated power supply• B inocular and trinocular models are supplied with an adjustable 1 W NeoLED™ illumination system for increased light output• M odels EC.1151, EC.1152, EC.1153, EC.1652 and EC.1653 are supplied with rechargeable batteries and an external 100-240 V battery charger/mains adapter• T he models for polarization are standard supplied with a 20 W halogen illumination for correct color rendering and an integrated 100-240 V power supply; on special requestThe models for polarization are supplied with H-LED illumination for correct color rendering (very similar to the halogen light spectrum) and an integrated 100-240 V power supply. However 20 W halogen illumination is also available on special requestPAC K AG I N G• S upplied with power cord or mains adapter-charger, dust cover, spare fuse, user manual and 5 ml immersion oil for models with an S100x objective • M odels for polarization with a halogen illumination are supplied with blue filter, models with LED illumination are supplied with white filter• All packed in a polystyrene box DIGI TAL MODEL SC A M E R A• A ll the EcoBlue digital microscopes are equipped with a 3.2 MP CMOS USB-2 camera• M aximum resolution 2048 ×1635, 24 bits color depth, up to 10 frames per second• D elivered with the ImageFocus Plus software, USB-2 cable and a micrometer 1mm/100 slide• Warranty for the camera is 2 yearsSO F T WA R E• Delivered with ImageFocus Plus for capturing of images and videos• T he capture and analysis ImageFocus Plus software allows to save images in .jpg, .tif or .bmp formats and videos in .avi format• I mages can be annotated and measurements can be performed on live or captured images• C ompatible with Windows 7, 8 and 10, both 32 and 64 bits configurations • A Mac OS version is also available• Free updates can be downloaded from our websiteEC.1001-P-HLEDEC.1152(1) Integrated mechanical stage with 70 x 27 mm X-Y travel range(2) With an external 100-240 V battery charger/mains adapter(3) P olarization models can also be supplied with S60x or S100x objectives;replace second digit of model number with resp. 6 or 1, e.g. EC.1602-P-HLED, EC.1103-P-HLED, etc ACCESSOR IES AND SPAR E PAR TSE Y E P I E C E SAE.5571 Wide field eyepiece WF 5x/18 mmEC.6010 HWF 10x/18 mm eyepieceEC.6010-M Wide field HWF 10x/18 mm eyepiece with micrometer EC.6015 HWF 15x/12 mm eyepieceEC.6110 HWF 10x/18 mm eyepiece with micrometerEC.6099 Pair of eyecupsO B J E C T I V E SEC.7004 Achromatic 4x/0.10 objective. Working distance 37.5 mm EC.7010 Achromatic 10x/0.25 objective. Working distance 6.8 mm EC.7020 Achromatic 20x/0.40 objective. Working distance 2.0 mm EC.7040 Achromatic S40x/0.65 objective. Working distance 0.6 mm EC.7060 Achromatic S60x/0.85 objective. Working distance 0.185 mm EC.7000 A chromatic S100x/1.25 oil immersion objective.Working distance 0.195 mmP O L A R I Z AT I O N AT TAC H M E N T SAE.5150 S imple polarization attachment. Polarizer 18 mm diameter mounted in eyepiece, analyzer 32 mm diameter placed infilter holderAE.5152 S imple polarization attachment. Analyzer mounted over eyepiece and analyzer 32 mm diameter in filter holder AE.5153 S imple polarization attachment. Analyzer mounted over eyepiece and 360° rotatable polarizer on table AE.5154 Polarizer in mount, to place over eyepieceAE.5155-P P olarizaton kit with analyzer in slider and small rotatable (100 x65 mm) stage with two clamps to be mounted on plain stage ofEcoBlue. Polarization filter to be mounted on the lamphouse AE.5158 P olarization kit with analyzer in slider and small rotatable(75 x 50 mm) stage with two clamps to be mounted on X-Y stageof EcoBlue. 360° Rotatable polarization filter to be mounted onthe lamphouseAE.5158-P P olarization kit with analyzer in slider and small rotatable(100 x 65 mm) stage with two clamps to be mounted on plainstage of EcoBlue. 360° Rotatable polarization filter to be mountedon the lamphouseC A M E R A ACC E SS O R I E SAE.5130 U niversal SLR camera adapter with 2x projection lens for23.2 mm tubes. Need T2 adapterAE.5025 T2 a dapter for Nikon D digital SLR camerasAE.5040 T2 adapter for Canon EOS digital SLR cameraOther T2 adapters on requestM I SC E L L A N E O U SEC.9500 A ttachable mechanical X-Y stage, translationrange 51 x 26 mm, with double Vernier andseparate horizontal adjustments knobsEuromexMicroscopenbv•Papenkamp20•6836BDArnhem•TheNetherlands•T+31(0)263232211•F+31(0)263232833•****************•EC.1005EC.9710 Frosted white filter EC.9981 1 W LED replacement unit EC.9991 1 W NeoLED™ replacement unit EC.9994 1 W H-LED replacement unit EC.9975 E xternal mains/charger adapter100-240 Vac/5 Vdc (50/60 Hz)AE.5511 Discussion head AE.5202 Blue filter, diameter 32 mm AE.5203 Yellow filter, diameter 32 mm AE.5204 Neutral grey filter, diameter 32 mm AE.5205 Green filter, diameter 32 mm AE.5207 Blue filter plexiglass, diameter 32 mm SL.1379 S pare halogen 20 W 12 V bulbfor polarization modelsAE.9918 Nylon microscope bag (25 x 39 x 19 cm)D I SP O SA B LE SPB.5157-W M icroscope slides 76 x 26 mm, ground edges.White frosted side, 50 pieces per packPB.5157-B M icroscope slides 76 x 26 mm, ground edges.Blue frosted side, 50 pieces per packPB.5155 Microscope slides 76 x 26 mm, ground edges, 50 pieces PB.5165 Cover glasses 18 x 18 mm, thickness 0.13-0.17 mm, 100 pieces PB.5168 Cover glasses 22 x 22 mm, thickness 0.13-0.17 mm, 100 pieces PB.5245 Lens cleaning paper, 100 sheets per pack PB.5255 Immersion oil, n = 1.482 (25 ml) PB.5274 Isopropyl alcohol 99% (200 ml)PB.5275 C leaning kit: lens cleaning fluid, lint free lens tissue, brush,air blower, cotton swabsPB.5276 M icroscope maintenance and servicing kit, 16pcs: cleaningbrush, 6 pcs screwdriver set, air blower, 3 pcs Allen key, 1.5, 2, 2.5 mm, lens cleaning fluid 20 ml, cleaning cloth 140 x 140 mm, 100 pcs Lens tissue sheets, tube of maintenance grease, 10 ml bottle of oil, packed in a nice toolbox。
sciencell内皮细胞培养基说明书
sciencell内皮细胞培养基说明书Sciencell内皮细胞培养基是一种特异性和高效性培养内皮细胞的基质，能够有效地维持和生长内皮细胞，并促进它们的分化和功能发挥。
以下是Sciencell内皮细胞培养基的详细说明：一、培养基配方Sciencell内皮细胞培养基主要包含以下成分：1. DMEM/F12培养基2. 10% FBS3. 内皮细胞生长因子（EGF、VEGF、FGF、呋喃丙酸等）4. 抗生素/抗菌素（penicillin和streptomycin）5. L-酪氨酸、谷氨酰胺和葡萄糖等。
二、适用范围Sciencell内皮细胞培养基适用于以下内皮细胞的培养：1. 人类内皮细胞（HEC、HUAEC、HPMEC等）2. 小鼠内皮细胞（MEC、MAEC等）3. 大鼠内皮细胞（REPEC、RAEC等）4. 其他动物内皮细胞。
三、培养条件1. 培养温度：37℃2. CO2浓度：5%3. 外源性添加物：Sciencell内皮细胞培养基中已经包含了必要的营养因子、生长因子和抗生素/抗菌素，不需要额外添加外源性添加物。
4. 培养密度：内皮细胞的培养密度应当根据不同细胞类型和研究需要适当调整，一般为1-3x10^4 cells/cm2。
5. 培养时间：培养时间应当根据不同细胞类型和实验需要适当调整，一般为4-7天。
四、保存条件Sciencell内皮细胞培养基应当在4℃低温保存，避免阳光直射和冻结。
保存期长达6个月，但是在保质期内使用会更好。
打开后，建议在一个月内使用完毕。
五、使用说明1. 移液操作：使用Sciencell内皮细胞培养基时应当采用无菌条件下的移液操作，并避免出现气泡和异物。
2. 细胞接种：将内皮细胞接种到预先涂覆的细胞培养底物上，并根据需要进行细胞划分和培养状态监测。
3. 细胞增殖：使用Sciencell内皮细胞培养基可促进内皮细胞的增殖和生长，建议每3-4天更换新的培养基。
4. 细胞检测：在细胞培养期间需监测细胞的培养状态、生长状态和增殖情况。
高精度血液分析仪产品说明书
MCV
50.00 – 150.00 fL
PLT
3.0 – 3,000.0 X 103 μL
NRBC
0.00 – 600.00/100 WBC
Retic
0.000 – 30.000 %
Data Management Computer Database Quality Assurance
Microsoft Windows** XP 40,000 Patient results with Graphics QC with Levy-Jennings Graph, XB/XM for Moving Averages, Daily Check, Intelligent Quality Monitoring
Blood Banking Centrifugation
Chemistry Flow Cytometry
Hematology Immunoassay Information Systems Lab Automation Molecular Diagnostics Rapid Diagnostics
Superior Quality of Results • Multi-Angle Flow Cytometric Digital Morphology
Weight and Dimensions Specimen Processing Module
Pneumatic Supply
Depth 78.7 cm (31.0 in) 51.0 cm (20.0 in)
Width 76.2 cm (30.0 in) 21.6 cm (8.5 in)
Height 96.5 cm (38.0 in) 36.8 cm (14.5 in)
奥林匹斯ESG-100E电子手术生成器产品说明书
ELECTROSURGICAL GENERATOROLYMPUS ESG-100A new dimension in electrosurgerySimply smart.Procedural solutions make the differenceInternational endoscopy specialists have assessed the clinical performance of daily electrosurgical appli c a t ions in gastroenterology and pneumology. Combined with Olympus’ Imaging and EndoTherapy products, this new electro s urgical generator offers procedural solutions that are suitable for state-of-the art endoscopies.→T heir conclusion: the Olympus ESG-100 is an easy-to-use and safe generator empowering existing and future EndoTherapy instruments.Simply smart.From the leader in flexible endoscopyRevolutionary imaging technologies such as HDTV and NBI emphasises Olympus‘ leadership in flexibleendoscopy for the gastrointestinal tract and respiratory systems. In order to offer the pro c edural solutionsthat today‘s endoscopists are looking for, Olympus has focused on delivering excellence in electrosurgery.In collaboration with a worldwide network of research centres, universities and hospitals, Olympus hasdeveloped the new Electrosurgical Generator ESG-100. This innovative product meets your needs as well asthe highest international quality standards. Its output power of up to 120 watts and a variety of monopolar andbipolar modes for cutting and coagulation support all electrosurgical procedures in flexible endoscopy.Simply smart.Highest safety meets outstanding effectivenessOlympus ESG-100 incorporates technical features that provide excellent effectiveness and highest safety incutting and coagulation during endoscopic procedures.Olympus’ Rapid Spark Technology TM features High Power Cut Support (HPCS) to achieve imme d iatespark ignition and Fast Spark Monitor (FSM) to optimise cutting procedures in various tissue structures.Always as much as necessary; never as much as possible.→T he results: reduced leakage currents, reproducible tissue effects and enhanced protectionof EndoTherapy instruments.Olympus’ Versatile Coagulation Technology TM enables fast and effective desiccation as well as soft anddeep tissue coagulation in monopolar and bipolar modes.→T he results: controlled haemostasis as well as effective coagulation of larger tissue volumeswith end-of-procedure detection and acoustic feedback.Simply smart.powerresistanceCutting modes to meet your needsMonopolar and bipolar continuous cutting modes with low, medium or high thermal effects in tissue.→T he innovation: monopolar intermittent cutting mode with two different speed settings.Coagulation modes to meet your needsOne monopolar soft coagulation mode with minimal sticking for controlled desiccation. Two monopolar forcedcoagulation modes for fast and effective haemostasis with low and high power capabilities.→T he innovation: two modes suitable for deep tissue denaturation. With acoustic feedbackand automatic end-of-procedure detection.powerHPCSCuttingHPCSCuttingCoagulationPulseCut slowPulseCut fastSpecifications Specifications Specifications, design, and accessories are subject to change without any notice or obligation on the part of the manufacturer.Postbox 10 49 08, 20034 Hamburg, Germany Wendenstrasse 14–18, 20097 Hamburg, Germany Phone: +49 40 23773-0, Fax: +49 40 Always a clear viewOlympus ESG-100 has been designed to work together with the new peristaltic pump AFU-100. The foot pedal of the generator can also be used to activate the pump. With its maximum flow of 600ml/min, this roller pump is useful to clean the treatment site through the endoscopes’ instrument channel and to flush through EndoTherapy instruments. The AFU-100 offers fast tube insertion, three programmable pre-set flow rates, as well as a variable flow rate and follows the easy-to-use concept of the ESG-100.One system made for youAn easy-to-use interface and an intuitive selection of output modes with the additional foot pedal button are clear advantages of the Olympus ESG-100. The result: ‘hands-free’ operation while performing advanced procedures requiring frequent changes of the output mode. The ergonomic design of every part of the system makes the Olympus ESG-100 very user friendly. Even suitable for beginners.The system fits well in all standard Olympus trolleys and carts. Its compact design meets the requirements for space saving medical devices and for an advanced workflow in today’s EndoSuites.Simply smart.Technical data Olympus ESG-100 High frequency 330 – 380 kHz Type, protection class CF, Class I Power supply 220–240V (WB991036), 100–120V (WB991046), 50/60Hz, 400VA Size (W ×D ×H) 295×375×115mm Weight, without footswitch 6.5kg Monopolar output Sockets ∅ 6mm A-cord Single/split neutral electrode 10mm 2-pins Cut 1/2/3 120W @ 500 Ohms PulseCut slow/fast 120W @ 500 Ohms SoftCoag 120W @ 100 Ohms ForcedCoag 1 50W @ 500 Ohms ForcedCoag 2 120W @ 500 Ohms Bipolar output Sockets 28.8mm 2-pins and ∅ 4/8 mm coaxial Cut 1/2/3 120W @ 500 OhmsSoftCoag 120W @ 100 Ohms RFCoag incl. RCAP 40W @ 100 OhmsOlympus AFU-100 Flow rate 10 – 270ml/min (Pump tube ID3.2mm) 20 – 600ml/min (Pump tube ID4.8mm)Pump head 4 rollers, detachable Type, protection class CF, Class I Power supply 100–240V, 50/60Hz, 60VA Size (W ×D ×H), incl. pump head 295×430×115mm Weight, without footswitch 5.6kg Ordering informationComponents Olympus ESG-100 (220–240V), including footswitch WB991036Olympus ESG-100 (100–120V), including footswitch WB991046Olympus AFU-100 (100–240V), including connection cable WB950167Footswitch for AFU-100 (optional) WB950242Pump tube ID3.2mm (15 pieces/box, sterile single use, length: 0.5m) WB920137Pump tube ID4.8mm (15 pieces/box, sterile single use, length: 0.5m) WB920138Order Number E 0429510 · 1.500 · 05/10 · P R。
拜科奇产品手册
目录
.1 . . . . . .1 .1.1 .1. .1. .1. .1. . ..1 .. .1 . . . . . . . 10 11
拜科奇的产品特性 ......................................................................................................... 质量保证........................................................................................................................ 产品纯度........................................................................................................................ 耐受性和安全性的临床前证据 ....................................................................................... 临床前免疫原性模型...................................................................................................... 临床前疗效研究............................................................................................................. 拜科奇临床前研究小结 .................................................................................................. 临床研究........................................................................................................................ 对既往接受过治疗的患者（PTPs）的研究.................................................................... 0 药代动力学 .................................................................................................................... 0 疗效............................................................................................................................... 临床免疫原性 ................................................................................................................ 外科手术中的应用 ......................................................................................................... 安全性 ........................................................................................................................... 对既往未接受过治疗（PUPs）或仅接受过最低限度治疗（MTPs）的患者的研究 ....... 拜科奇用于治疗PUPs/MTPs的安全性及疗效 ................................................................ PUPs/MTPs中抑制物的产生率...................................................................................... 重组人凝血因子VIII抑制物的形成.................................................................................. 1 抑制物形成的潜在标志物 .............................................................................................. 1 抑制物形成的发生率...................................................................................................... 抑制物分子 ................................................................................................................... 抑制物的特异性............................................................................................................. 对PTPs的临床免疫原性研究 ......................................................................................... PUPs/MTPs中抑制物发生率的比较 ............................................................................. 抑制物产生的累积风险 .................................................................................................. 0 小结：拜科奇与凝血因子VIII抑制物 .............................................................................. 1 免疫耐受诱导 (ITI) ........................................................................................................ 长期预防........................................................................................................................ 产品亮点总结 ................................................................................................................ 0 参考文献........................................................................................................................
可溶性血小板内皮细胞粘附分子1ELISA试剂盒说明书
可溶性血小板内皮细胞粘附分子1ELISA试剂盒说明书可溶性血小板内皮细胞粘附分子1ELISA试剂盒说明书上海樊克生物供应！产品规格：96T/48T供应商：上海樊克生物有限公司本产品只用于科研实验上海樊克生物供应！可溶性血小板内皮细胞粘附分子1ELISA试剂盒说明书试剂的准备：按试剂盒说明书的要求准备实验中需用的试剂。
ELISA中用的蒸馏水或去离子水，包括用于洗涤的，应为新鲜的和高质量的。
自配的缓冲液应用pH计测量较正。
从冰箱中取出的试验用试剂应待温度与室温平衡后使用。
试剂盒中本次试验不需用的部分应及时放回冰箱保存。
ELISA试剂盒的优势：全面——混合 8 种不同的常见抗原，对自身免疫性疾病进行全面筛查。
快速——检测时间短( ~2 小时)，确诊时间更早准确——ELISA 检测方法，灵敏度高，特异性强，适用性好。
质优——美国技术开发，高品质及高可靠性的分析性能。
价低——国内自生产，符合国内实情，降低生产成本。
可溶性血小板内皮细胞粘附分子1ELISA试剂盒说明书试剂盒组成1 30 倍浓缩洗涤液 20ml×1 瓶；2 酶标试剂 6ml×1 瓶3 酶标包被板 12 孔×8 条；4 样品稀释液 6ml×1 瓶5 显色剂A 液 6ml×1 瓶；6 显色剂B 液 6ml×1/瓶7 终止液 6ml×1 瓶；8 标准品（48ng/ml） 0.5ml×1 瓶9 标准品稀释液 1.5ml×1 瓶；10 说明书 1 份11 封板膜 2 张； 12 密封袋 1国产/进口Elisa试剂盒，96T/48TElisa试剂盒， 96T/48Telisakit价格可溶性血小板内皮细胞粘附分子1ELISA试剂盒说明书Operational matters needing attention1 reagents should be stored according to the label instructions, and return to room temperature before use. After thinning of the standard products should be discarded, can not be saved.2 in the experiment, the need for the slab should be immediately put back in the bag, sealed to avoid deterioration.3 other reagents should be packaged or covered. Do not mix different batches of reagents. Before use.4 use disposable suction head to avoid cross contamination, draw the termination solution and substrate A, B liquid, avoid using the belt with metal part of the sample.5 use a clean plastic container to configure the washing liquid. All samples of ingredients and mix well before use in the kit.6 wash the enzyme labeled plate should be fully dry, do not put water absorbent paper directly into the enzyme labeled reaction pore water.7 substrate A should be volatile, avoid long time to open the lid. Substrate B is sensitive to light and avoid prolonged exposure to light. Avoid hand contact, toxic. After completion of the experiment should be immediately read od.8 add reagents should be consistent in order to ensure that all reaction plate hole incubation time.9 according to the time specified in the instructions, the amount and the order of the increase in the temperature of the operation.可溶性血小板内皮细胞粘附分子1ELISA试剂盒说明书样品收集、处理及保存方法1、血清-----操作过程中避免任何细胞刺激。
希森美康血凝仪
CS-2100i/2000i根据SYSMEX在血栓与止血领域多年积累的经验，为全面满足各类实验室的需求率先开发CS-2100i/2000i。
四种方法学：凝固法/发色底物法/免疫比浊法/聚集法unique!多波长高精度光学检测：5种波长检测提供可靠数据新型智能监测：特有的HIL check 功能排除溶血/黄疸/脂血干扰更多新检测项目的选择：FXIII，vWF:Rco等更可靠的实验室质量保证：SNCS实时在线质控全面满足血栓与止血检测需求！CA-7000以全球最快的分析速度提供全面和高精准的止凝血项目检测结果，仪器集中了凝固法，发色底物法和免疫法于一体，设计高度人性化和智能化，操作简便，成为大规模实验室的首选。
● 多参数测试，500测试/小时高速分析能力● 操作简便，灵活对待各种需求● 优秀的试剂管理系统（SRS）● 安全、实用的系统设计● 大容量的数据管理能力，完整的质控系统CA-1500汇集了当今血栓/止血分析仪最新的各种先进功能于一身，是市场上少见的性能/价格比极高的一台仪器，是大型教学医院，综合医院实验室的首选。
它具有快速处理能力，最快180测试/小时，集多种检测功能于一身：凝固法、发色底物法、免疫法。
具有全能随机组合能力，两种方法测定纤维蛋白质，适合常规大量和急诊使用。
● 拥有高速处理能力、随机测试功能和自动再检查功能● 三种分析方式，包括多规则监视的广泛质控文件和平行线生物分析功能● 卓越的性能可以灵活适应实验室的多样化需求CA-500系列CA-500系列包含了六款机型，设计新颖、符合经济原则，是各中小型实验室开展血栓/止血实验的最佳选择，也是半自动升级到全自动的理想机型。
小型台式仪实用可靠,具备三种检测系统即凝固法、发色底物法、免疫法的自由组合用户可根据需要选择相应机型。
CA-50设计上完全沿用了全自动CA系列的检测原理，锁定人为误差因素的设计确保它有别于其他半自动血凝仪，达到全自动仪器的准确性与重复性效果，四通道即可批量检测又可单独检测，内置质控文件，适用于小标本量实验室使用。
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Endothelial Cell Medium
(ECM)
Catalog #1001 Product Description
Endothelial Cell Medium (ECM), when used with Endothelial Cell Growth Supplement (ECGS, Cat #1052) and 25 ml of fetal bovine serum (FBS) is a complete medium designed for optimal growth of normal endothelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal endothelial cells in vitro.
Components
ECM consists of 500 ml of basal medium, 25 ml of FBS (Cat. #0025), 5 ml of Endothelial Cell Growth Supplement (ECGS, Cat. #1052) and 5 ml of penicillin/streptomycin solution (P/S, Cat. #0503). Note: FBS, ECGS and P/S are not pre-mixed in ECM; they must be added separately to make the complete ECM.
Product Use
ECM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium at 4o C and the ECGS, FBS and P/S solution at -20o C. Protect from light. Shipping
Basal medium: room temperature. Supplements: dry ice.
Instructions for Use
Thaw ECGS, FBS and P/S solution at 37o C. Gently tilt the tubes several times to ensure the contents are completely mixed before adding to the medium. Spray the medium bottle and tubes with 70% ethanol, and wipe to remove excess liquid. In a sterile field, remove the caps without touching the interior threads with fingers. Add ECGS, FBS and P/S solution to the medium and mix well. Since several components are light-labile, the medium should not be exposed to light for extended periods. We do not recommend warming medium in a 37o C water bath prior to use. When stored in the dark at 4o C, the reconstituted medium is stable for one month.
Caution: If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.。