迈瑞试剂作业指导书
迈瑞BS-400使用说明书（二）
图2-1分析部背面⏹DEIONIZED WATER：仪器后部进水口，进水管由此进入仪器；⏹WASTE1：高浓度废液排出口，高浓度废液管由此引出；⏹WASTE2：低浓度废液排出口，低浓度废液管由此引出；⏹COM：仪器主机（分析部）同控制计算机通信的串口；⏹WAN：仪器主机（分析部）同控制计算机通信的网口。
1.1.1.1 样本盘系统样本盘系统主要包括样本盘（含样本盘盖）。
样本盘是装载样本试管的支架，将每个样本试管转到样本针的吸样位，等待吸样。
如图2-2所示。
样本盘仅作逆时针旋转，将指定的样本送至样本针吸样位置。
图2-2 样本盘样本盘分为内、中、外三圈，每圈30个样本位，共90个样本位，其中：⏹常规位置为1~60；⏹急诊位置为E1~E10（76-85）；⏹定标位置为S1~S10（61-70）；⏹质控位置位C1~C5（71-75）；⏹其他位置：一个去离子水位W（蒸馏水清洗液，90），一个酸清洗剂位D1（ISE清洗液，87），一个碱清洗剂位D2（酸清洗液，88），一个电解质（ISE）清洗液D3（碱清洗液，89）。
稀释好的尿液选择放置在样本盘的常规样本位中任意一个，由用户使用过程中自定义。
虚拟样本盘：允许最大设置6个虚拟样本盘，默认一个样本盘；样本盘可放置原始采血管、离心管、塑料试管以及微量样品杯，支持手持式或固定式条码扫描。
样本盘兼容以下样本容器：⏹微量样本杯：Φ12×37mm、Φ14×25mm ；⏹原始采血管：Φ12×68.5 mm ，Φ12×99 mm ，Φ12.7×75 mm ，Φ12.7×100 mm ，Φ13 X 75 mm ，Φ13 X 95 mm ，Φ13 X 100 mm ；⏹塑料试管：Φ12×68.5 mm ，Φ12×99 mm ，Φ12.7×75 mm ，Φ12.7×100 mm ，Φ13 X 75 mm，Φ13 X 95 mm ，Φ13 X 100 mm 。
迈瑞BC2800血常规SOP
血液常规检验标准操作规程一．目的:检测分析血液中红细胞、白细胞、血小板、血红蛋白的数量和质量，对感染、炎症、血液系统疾病等进行辅助诊断、监测治疗效果等。
2．检测项目:红细胞计数、血红蛋白、红细胞比积、平均红细胞体积、平均红细胞二．检测项目:红细胞计数、血红蛋白、红细胞比积、平均红细胞体积、平均红细胞血红蛋白含量、平均红细胞血红蛋白浓度、白细胞计数、白细胞分类、血小板计数。
三．标本的采集与运送1 .标本类型：静脉血或手指末梢血。
2.标本要求：标本用EDTA-K2 抗凝；静脉血标本量应达到2ml；末梢血20µl。
3.标本运送：室温运送，4小时内完成检测。
如不需PLT、MCV、和白细胞三分群的分析结果，可在2-8摄氏度的冰箱中保存24小时。
经过冷藏的样本应在室温下放置30分钟后才能进行分析。
4.标本拒收标准：严重溶血、凝固、血量少、无条码、无标识的血液标本不能进行检测。
四.标本的检测仪器及试剂1 .仪器：深圳mindrary公司BC—2800全自动血液细胞分析仪。
试剂：由mindrary公司提供包括LH溶血剂、LEO(Ⅰ)溶血剂、LBA溶血剂、LEO(Ⅱ)溶血剂、清洁液、稀释液、探头清洁液等配套试剂。
2.检测原理采用流式细胞技术，通过检测光学信号进行白细胞计数及白细胞分类测定；双鞘流电阻原理进行红细胞与血小板测定；HiCN-HGB法检测血红蛋白。
3.操作步骤3.1打开仪器电源开关，电源指示灯亮，屏幕显示Intializing,分析仪进入初始化，初始化结束后，系统自动进入“计数”界面。
注：在分析仪报“本底异常”故障时进行分析，将得到不可靠的结果，3.2在仪器进入样本分析前，每日需进行质控分析，以确保分析仪得到可靠的分析结果。
3.3全血-全参数模式a. 在仪器主界面点击“计数”，进入计数界面。
b. 点击计数界面“模式”，在弹出的对话框中选择“全血模式”。
录入样本信息，输入分析样本编号。
c. 上下颠倒试管将试管内容物充分混匀，轻轻取下盖子，防止血液溅出。
迈瑞BS-300
迈瑞-BS300上机操作：
1．仪器共反应10分钟，读50个点，12 s /点(BS-200是16s/点)。
孵育时间是指加入样本之后到加入R2试剂读点数，检测时间是
指以加入R2后为起点，初始读光点和结束读点。
终点法一般需
要从0点开始扣除空白吸光度，或者是从-1，-2开始，速率法
代表读点的时间段。
2．在输入新项目的参数后，需要在同一对话框的不同列中设置好参考范围，计算项目等等
3．试剂注册过程中，在试剂状态的右列选择需要设置新项目的R1,R2试剂位，然后一定要选择相应的试剂瓶型号，一般都是迈
瑞试剂大瓶，然后在左边对话框中选择新的项目和R1或是R2,
记得选择的试剂盘号码，不知道的话就向科室老师问问，或是
看看他们别的项目是怎么设置的。
4．在定标品的设置中，需要注意很多医院会把定标品专门设置一个试剂盘。
这样，对于位置号就要选择相应的试剂盘这一行，
如果仪器本身提供的标准试剂位不够，可以使用其它，然后在
下拉表中找到欲放置的位置。
然后是定标品浓度的设置，项目
的设置，该款仪器允许操作者手工设置复合定标品。
5．该款仪器在定标过程中，如果个别点的定标失败，仪器停下来后会有个提示，这时候，不需要重新定标，可以点击上行的运
行，再选择针对部分定标品重新定标，这样就避免了已完成的
定标点重新定标的麻烦。
6．定标时，在样本位的W位是默认的水位，不必在定标品的设置中专门再放置一个水位，不管有没有设置W位的浓度，系统均会有一个水的空白定标。
如Loggit4P的定标模式中，只需要设置4个含有浓度的定标品浓度。
迈瑞 SED035Mu 96 检测 酶联免疫吸附试剂盒 说明书
SED035Mu96TestsEnzyme-linked Immunosorbent Assay KitFor Syntaxin1A,Brain(STX1A)Organism Species:Mus musculus(Mouse)Instruction manual FOR IN VITRO AND RESEARCH USE ONLYNOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES11th Edition(Revised in July,2013)[INTENDED USE]The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of STX1A in mouse tissue homogenates and other biological fluids.[REAGENTS AND MATERIALS PROVIDED]Reagents Quantity Reagents Quantity Pre-coated,ready to use96-well strip plate1Plate sealer for96wells4Standard2Standard Diluent1×20mL Detection Reagent A1×120μL Assay Diluent A1×12mL Detection Reagent B1×120μL Assay Diluent B1×12mL TMB Substrate1×9mL Stop Solution1×6mL Wash Buffer(30×concentrate)1×20mL Instruction manual1 [MATERIALS REQUIRED BUT NOT SUPPLIED]1.Microplate reader with450±10nm filter.2.Precision single or multi-channel pipettes and disposable tips.3.Eppendorf Tubes for diluting samples.4.Deionized or distilled water.5.Absorbent paper for blotting the microtiter plate.6.Container for Wash Solution[STORAGE OF THE KITS]1.For unopened kit:All the reagents should be kept according to the labels on vials.The Standard,DetectionReagent A,Detection Reagent B and the96-well strip plate should be stored at-20o C upon receipt while the others should be at4o C.2.For opened kit:When the kit is opened,the remaining reagents still need to be stored according to theabove storage condition.Besides,please return the unused wells to the foil pouch containing the desiccant pack,and reseal along entire edge of zip-seal.Note:It is highly recommended to use the remaining reagents within1month provided this is within the expiration date of the kit.For the expiration date of the kit,please refer to the label on the kit box.All components are stable until this expiration date.[SAMPLE COLLECTION AND STORAGE]Tissue homogenates-The preparation of tissue homogenates will vary depending upon tissue type.For this assay,tissues were rinsed in ice-cold PBS(0.01mol/L,pH7.0-7.2)to remove excess blood thoroughly and weighed before homogenization.Minced the tissues to small pieces and homogenized them in5-10mL of PBS with a glass homogenizer on ice(Micro Tissue Grinders woks,too).The resulting suspension was sonicated with an ultrasonic cell disrupter or subjected to two freeze-thaw cycles to further break the cell membranes.After that,the homogenates were centrifugated for5minutes at5000×g.Remove the supernate and assay immediately or aliquot and store at≤-20o C.Other biological fluids-Centrifuge samples for20minutes at1000×g.Remove particulates and assay immediately or store samples in aliquot at-20o C or-80o C for later use.Avoid repeated freeze/thaw cycles. Note:1.Samples to be used within5days may be stored at4o C,otherwise samples must be stored at-20o C(≤1month)or-80o C(≤2months)to avoid loss of bioactivity and contamination.2.Sample hemolysis will influence the result,so hemolytic specimen should not be detected.3.When performing the assay,bring samples to room temperature.[REAGENT PREPARATION]1.Bring all kit components and samples to room temperature(18-25o C)before use.2.Standard-Reconstitute the Standard with1.0mL of Standard Diluent,kept for10minutes at roomtemperature,shake gently(not to foam).The concentration of the standard in the stock solution is40ng/mL.Please firstly dilute the stock solution to10ng/mL and the diluted standard serves as the highest standard (10ng/mL).Then prepare7tubes containing0.5mL Standard Diluent and use the diluted standard to producea double dilution series according to the picture shown below.Mix each tube thoroughly before the nexttransfer.Set up7points of diluted standard such as10ng/mL,5ng/mL,2.5ng/mL,1.25ng/mL,0.625ng/mL,0.312ng/mL,0.156ng/mL,and the last EP tubes with Standard Diluent is the blank as0ng/mL.Tube123456789 ng/mL40105 2.5 1.250.6250.3120.15603.Detection Reagent A and Detection Reagent B-Briefly spin or centrifuge the stock Detection A andDetection B before use.Dilute to the working concentration with Assay Diluent A and B,respectively (1:100).4.Wash Solution-Dilute20mL of Wash Solution concentrate(30×)with580mL of deionized or distilled waterto prepare600mL of Wash Solution(1×).5.TMB substrate-Aspirate the needed dosage of the solution with sterilized tips and do not dump the residualsolution into the vial again.Note:1.Making serial dilution in the wells directly is not permitted.2.Prepare standard within15minutes before assay.Please do not dissolve the reagents at37o C directly.3.Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction,and avoid foaming and mix gently until the crystals are completely dissolved.To minimize imprecision caused by pipetting,use small volumes and ensure that pipettors are calibrated.It is recommended to suck more than10μL for once pipetting.4.The reconstituted Standards,Detection Reagent A and Detection Reagent B can be used only once.5.If crystals have formed in the Wash Solution concentrate(30×),warm to room temperature and mix gentlyuntil the crystals are completely dissolved.6.Contaminated water or container for reagent preparation will influence the detection result.[SAMPLE PREPARATION]1.Cloud-Clone Corp.is only responsible for the kit itself,but not for the samples consumed during the assay.The user should calculate the possible amount of the samples used in the whole test.Please reserve sufficient samples in advance.2.Please predict the concentration before assaying.If values for these are not within the range of the standardcurve,users must determine the optimal sample dilutions for their particular experiments.3.If the samples are not indicated in the manual,a preliminary experiment to determine the validity of the kit isnecessary.4.Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results dueto the impacts from certain chemicals.5.Due to the possibility of mismatching between antigen from other origin and antibody used in our kits(e.g.,antibody targets conformational epitope rather than linear epitope),some native or recombinant proteins from other manufacturers may not be recognized by our products.6.Influenced by the factors including cell viability,cell number or sampling time,samples from cell culturesupernatant may not be detected by the kit.7.Fresh samples without long time storage is recommended for the test.Otherwise,protein degradation anddenaturalization may occur in those samples and finally lead to wrong results.[ASSAY PROCEDURE]1.Determine wells for diluted standard,blank and sample.Prepare7wells for standard,1well for blank.Add100μL each of dilutions of standard(read Reagent Preparation),blank and samples into the appropriate wells.Cover with the Plate sealer.Incubate for2hours at37o C.2.Remove the liquid of each well,don’t wash.3.Add100μL of Detection Reagent A working solution to each well.Incubate for1hour at37o C after coveringit with the Plate sealer.4.Aspirate the solution and wash with350μL of1×Wash Solution to each well using a squirt bottle,multi-channel pipette,manifold dispenser or autowasher,and let it sit for1~2minutes.Remove the remaining liquid from all wells completely by snapping the plate onto absorbent paper.Totally wash3times.After the last wash,remove any remaining Wash Buffer by aspirating or decanting.Invert the plate and blot it against absorbent paper.5.Add100μL of Detection Reagent B working solution to each well.Incubate for30minutes at37o C aftercovering it with the Plate sealer.6.Repeat the aspiration/wash process for total5times as conducted in step4.7.Add90μL of Substrate Solution to each well.Cover with a new Plate sealer.Incubate for15-25minutes at37o C(Don't exceed30minutes).Protect from light.The liquid will turn blue by the addition of Substrate Solution.8.Add50μL of Stop Solution to each well.The liquid will turn yellow by the addition of Stop solution.Mix theliquid by tapping the side of the plate.If color change does not appear uniform,gently tap the plate to ensure thorough mixing.9.Remove any drop of water and fingerprint on the bottom of the plate and confirm there is no bubble on thesurface of the liquid.Then,run the microplate reader and conduct measurement at450nm immediately. Note:1.Assay preparation:Keep appropriate numbers of wells for each experiment and remove extra wells frommicroplate.Rest wells should be resealed and stored at-20o C.2.Samples or reagents addition：Please use the freshly prepared Standard.Please carefully add samplesto wells and mix gently to avoid foaming.Do not touch the well wall.For each step in the procedure,total dispensing time for addition of reagents or samples to the assay plate should not exceed10minutes.This will ensure equal elapsed time for each pipetting step,without interruption.Duplication of all standards and specimens,although not required,is recommended.To avoid cross-contamination,change pipette tips between additions of standards,samples,and reagents.Also,use separated reservoirs for each reagent. 3.Incubation:To ensure accurate results,proper adhesion of plate sealers during incubation steps isnecessary.Do not allow wells to sit uncovered for extended periods between incubation steps.Once reagents are added to the well strips,DO NOT let the strips DRY at any time during the assay.Incubation time and temperature must be controlled.4.Washing:The wash procedure is plete removal of liquid at each step is essential for goodperformance.After the last wash,remove any remaining Wash Solution by aspirating or decanting and remove any drop of water and fingerprint on the bottom of the plate.Insufficient washing will result in poor precision and false elevated absorbance reading.5.Controlling of reaction time:Observe the change of color after adding TMB Substrate(e.g.observationonce every10minutes),if the color is too deep,add Stop Solution in advance to avoid excessively strong reaction which will result in inaccurate absorbance reading.6.TMB Substrate is easily contaminated.Please protect it from light.7.The environment humidity which is less than60%might have some effects on the final performance,therefore,a humidifier is recommended to be used at that condition.[TEST PRINCIPLE]The microtiter plate provided in this kit has been pre-coated with an antibody specific to STX1A.Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to STX1A.Next,Avidin conjugated to Horseradish Peroxidase(HRP)is added to each microplate well and incubated.After TMB substrate solution is added,only those wells that contain STX1A,biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of450nm±10nm.The concentration of STX1A in the samples is then determined by comparing the O.D.of the samples to the standard curve.[CALCULATION OF RESULTS]Average the duplicate readings for each standard,control,and samples and subtract the average zero standard optical density.Construct a standard curve by plotting the mean O.D.and concentration for each standard and draw a best fit curve through the points on the graph or create a standard curve on log-log graph paper with STX1A concentration on the y-axis and absorbance on the ing some plot software,for instance,curve expert1.30,is also recommended.If samples have been diluted,the concentration read from the standard curve must be multiplied by the dilution factor.[TYPICAL DATA]In order to make the calculation easier,we plot the O.D.value of the standard(X-axis)against the known concentration of the standard(Y-axis),although concentration is the independent variable and O.D.value is the dependent variable.However,the O.D.values of the standard curve may vary according to the conditions of assay performance(e.g.operator,pipetting technique,washing technique or temperature effects),plotting log of the data to establish standard curve for each test is recommended.Typical standard curve below is provided for reference only.Typical Standard Curve for STX1A,Mouse ELISA.[DETECTION RANGE]0.156-10ng/mL.The standard curve concentrations used for the ELISA’s were10ng/mL,5ng/mL,2.5ng/mL,1.25ng/mL,0.625ng/mL,0.312ng/mL,0.156ng/mL.[SENSITIVITY]The minimum detectable dose of STX1A is typically less than0.059ng/mL.The sensitivity of this assay,or Lower Limit of Detection(LLD)was defined as the lowest protein concentration that could be differentiated from zero.It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration. [SPECIFICITY]This assay has high sensitivity and excellent specificity for detection of STX1A.No significant cross-reactivity or interference between STX1A and analogues was observed.Note:Limited by current skills and knowledge,it is impossible for us to complete the cross-reactivity detection between STX1A and all the analogues,therefore,cross reaction may still exist.[PRECISION]Intra-assay Precision(Precision within an assay):3samples with low,middle and high level STX1A were tested 20times on one plate,respectively.Inter-assay Precision(Precision between assays):3samples with low,middle and high level STX1A were tested on3different plates,8replicates in each plate.CV(%)=SD/meanX100Intra-Assay:CV<10%Inter-Assay:CV<12%[STABILITY]The stability of ELISA kit is determined by the loss rate of activity.The loss rate of this kit is less than5%within the expiration date under appropriate storage condition.To minimize extra influence on the performance,operation procedures and lab conditions,especially room temperature,air humidity,incubator temperature should be strictly controlled.It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.[ASSAY PROCEDURE SUMMARY]1.Prepare all reagents,samples and standards;2.Add100μL standard or sample to each well.Incubate2hours at37o C;3.Aspirate and add100μL prepared Detection Reagent A.Incubate1hour at37o C;4.Aspirate and wash3times;5.Add100μL prepared Detection Reagent B.Incubate30minutes at37o C;6.Aspirate and wash5times;7.Add90μL Substrate Solution.Incubate15-25minutes at37o C;8.Add50μL Stop Solution.Read at450nm immediately.[IMPORTANT NOTE]1.Limited by the current condition and scientific technology,we can't completely conduct the comprehensiveidentification and analysis on the raw material provided by suppliers.So there might be some qualitative and technical risks to use the kit.2.The final experimental results will be closely related to validity of the products,operation skills of the endusers and the experimental environments.Please make sure that sufficient samples are available.3.Kits from different batches may be a little different in detection range,sensitivity and color developing time.Please perform the experiment exactly according to the instruction attached in kit while electronic ones from our website is only for information.4.Do not mix or substitute reagents from one kit lot to e only the reagents supplied by manufacturer.5.Protect all reagents from strong light during storage and incubation.All the bottle caps of reagents should becovered tightly to prevent the evaporation and contamination of microorganism.6.There may be some foggy substance in the wells when the plate is opened at the first time.It will not haveany effect on the final assay results.Do not remove microtiter plate from the storage bag until needed.7.Wrong operations during the reagents preparation and loading,as well as incorrect parameter setting for theplate reader may lead to incorrect results.A microplate plate reader with a bandwidth of10nm or less and an optical density range of0-3O.D.or greater at450±10nm wavelength is acceptable for use in absorbance measurement.Please read the instruction carefully and adjust the instrument prior to the experiment.For more information,please refer to the operation video(/homepage/operate.htm). 8.Even the same operator might get different results in two separate experiments.In order to get betterreproducible results,the operation of every step in the assay should be controlled.Furthermore,a preliminary experiment before assay for each batch is recommended.9.Each kit has been strictly passed Q.C test.However,results from end users might be inconsistent with ourin-house data due to some unexpected transportation conditions or different lab equipments.Intra-assay variance among kits from different batches might arise from above factors,too.10.Kits from different manufacturers with the same item might produce different results,since we haven’tcompared our products with other manufacturers.11.The instruction manual also suits for the kit of48T,but all reagents of48T kit are reduced by half. [PRECAUTION]The Stop Solution suggested for use with this kit is an acid solution.Wear eye,hand,face,and clothing protection when using this material.[TROUBLE SHOOTING]Problem Possible Source Correction ActionPoor Standard Curve Improper standard curve preparation Ensure accurate operation of the dilution Incomplete washing and aspiration Adequate washing and adequate aspiration Inaccurate Pipetting Check and Calibrate pipettesPoor Precision Incomplete washing of wells Ensure sufficient washingInadequate mixing and aspiration reagents Adequate aspiration and mixing reagentsReused pipette tips,containers and sealers Change and use new pipette tips,containers and sealers Inaccurate Pipetting Check and Calibrate pipettesLow O.D Values Inadequate reagent volumes added to wells Calibrate pipettes and Add adequate reagentsIncorrect incubation times Ensure sufficient incubation timesIncorrect incubation temperature Reagents balanced to room temperatureConjugate or substrate reagent failure Mix conjugate&substrate,color should develop immediately No stop solution added Follow the assay protocol in the kit manualRead beyond suggested reading time Read within the time recommended in the manualSample Values Improper Sample Storage Store the sample properly and use the fresh sample Improper sample collection and preparation Take proper sample collection and preparation method Low quantity of analyte in samples Use new sample and repeat assay。
迈瑞铁蛋白检测试剂盒说明书
Manual ProcedureAutomated procedure on requestTest PrincipleFerric ions (Fe +3) are released from transferring under acidicconditions and reduced to ferrous state (Fe +2) by a strong reducing agent. Ferrous ions then react with Ferrozine to form a coloredcomplex which can be measured photometrically. The intensity of the color produced is proportional to the iron concentration in the sample. Lipemic samples are clarified by the detergent.Transferrin-Fe-complex apotransferrin + Fe 3+Fe 3+ Fe 2+Ferrozine + Fe 2+ colored complexStability and preparation of working reagentBuffer Reagent B-R1: liquid Powder Reagent P-R1: powder. Reagent R2: liquid, ready to useWorking ReagentAdd 10 ml reagent Buffer B-R1 to one vial powder reagent P-R1 andmix gently for 15 minutes before use.Stability: 2 weeks at 20 – 25 °C1 month at2 – 8 °CAll reagents are stable up to expiry date given on label when storedat 2 – 8 °CNote : All reagents should be clear solutions. Don’t use if the reagent is turbid. Specimen Collection and Handling1. Non-hemolyzed serum is the specimen of choice.2. Serum should be separated from blood clot as soon as possible.3. Heparinized plasma could only be used, other anticoagulantsshould not be used.4. Serum iron is reported to be stable for 4 days at 20 – 25°C and7 days at 2 – 8 °C.CalibratorMediCal U Cat. No. 15011Iron STD. Cat. No. 16131Quality controlMeditrol N Cat. No. 15171 Meditrol P Cat. No. 15181Iron (Ferrozine)Colorimetric testPowder liquid reagentsCalculationConc.Iron = ⨯ Conc. Standardμμ g /dlLinearity Up to 1000 μg/dl (179 μmol/L); If result exceeds 1000 μg/dl, repeat test using diluted sample (1+1) with sodium chloride solution (0.9 %) and multiply result by 2.Interference 1. Certain drugs and other substances are known to influencecirculating iron levels. 2. To make tubes, pipettes, etc. iron free, they must be washed with diluted (1+2) hydrochloric or nitric acid followed by several rinsings with iron free deionized or distilled water.Precautions 1. The reagent is toxi c, don’t pipette by mouth, avoid all contacts. 2. Use only disposable plastic containers or iron free tubes andcuvettes. Avoid any contamination by the use of clean laboratory material.Cat. No. 12881 B-R1 1 Buffer 50mlFor 50 testsP-R1 5 powder for 10 mlR2 1 x 10 ml(A S -A SB ) SampleA standardGuanidinium PH=4.5 Ascorbic acidIron ferrozine methodReference rangeReferences1. Garoc A., Clin. Chem. Acta 94, 115 (1979).2. Brivio et coll., La Ricerca Clin. Lab. 18, 523 (1986).3. Young, DS., Effects of Drugs on Clinical Laboratory Tests, fifthedition 2000, AACC Press, Washington, D.C.。
(二)迈瑞5300操作规程MicrosoftWord文档
迈瑞BC-5300 型血细胞分析仪操作维护规程1．仪器原理及分析参数1.1原理：采用流式细胞技术，通过检测光学信号进行白细胞计数及白细胞分类测定；双鞘流电阻原理进行红细胞与血小板测定；SLS -HGB法检测血红蛋白。
1.2分析参数：WBC, RBC, HGB, HCT, MCV, 1 ．仪器原理及分析参数1.1原理：采用流式细胞技术，通过检测光学信号进行白细胞计数及白细胞分类测定；双鞘流电阻原理进行红细胞与血小板测定；SLS -HGB法检测血红蛋白。
1.2分析参数：WBC, RBC, HGB, HCT, MCV, MCH, MCHC, PLT,NEUT%,LYMPH%M, ONO%E,O%,BASO%,NEUT#, LYMPH#,MONO#,EO#, BASO#, RDW-SD, RDW-CV, PDW, MPV2.仪器性能参数2.1仪器运行环境环境温度：15-30 C （23 C最适）相对湿度：30 %〜85 %2.2技术规格电源供给：主机220-240V±10%(50/60)Hz保护类型：绝缘协调：II类电压，2度保护显示范围：WBC 0.00〜999.99( X 109/L)RBC 0.00 〜99.99( X 1012/L)HGB 0.0 〜300.0(g/L)PLT 0〜9999(X109/L)分析范围： WBC 0.00~100.00 (109/L)RBC 0.00~10.00 (1012/L)HGB 0〜250(g/L) PLT 0〜1000 (109/L)仪器模式： 全血模式 分析仪将吸取 20卩L （ CBC+DIFF 模式）或15卩L （ CBC 模式）的全血样本。
预稀释模式 分析仪将吸入80卩L （ CBC+DIFF 模式）或40 卩L （ CBC 模式）的稀释样本。
3. 试剂及贮存条件3.1 试剂：由 mindrary 公司提供包括 M-53LH 溶血剂、M-50LEO （n ）溶血剂、M-53 清洁液、M-53 稀释液的配套试剂。
迈瑞BS300操作
然后到测试状态下选定样品盘号选中定标测试编译，点击全部选中编译测试开始。
注意：
1.定标模式若选Spline、Log4p、5p，定标时一定要有0点，且0点的浓度只能是0。
2.新试验项目必须做试剂空白
3.试验最好使用仪器厂商配套的比色杯
迈瑞BS-300
1、工作设置项目管理增加项目：设置参数
试剂管理：设置R1/R2位置
定标设置：
2、定标设置定标液管理增加定标液设置定标液浓度
设置样本位（定标液的位置）
5号样品盘“其他”选定位置（S1-S6）
测试设置测试申请参数
参与测试定标液
3、日常操作试剂空白样本盘（选定定标液放置的盘号）点击项目申请然后到测试状态下选定样品盘号选中空白测试编译，点击全部选中编译测试开始。
迈瑞BS-400使用说明书(一)
迈瑞BS-400使用说明书(一)BS-400全自动生化分析仪Chemistry AnalyzerOperation Manual知识产权本使用说明书及其对应产品的知识产权属于深圳迈瑞生物医疗电子股份有限公司(以下简称“迈瑞公司”)。
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篇一：化学试剂管理作业指导书篇二：化学试剂及标准滴定溶液的配制和标定作业指导书化学试剂及标准滴定溶液的配制和标定分析过程中，只应使用蒸馏水或同等纯度的水；所有试剂应为分析纯或优级纯试剂；用于标定与配制标准溶液的试剂，除另有说明外应为基准试剂。
除另有说明外，%表示"%（m/m）"。
本标准使用的市售浓液试剂具有下列密度（ρ）（20℃,单位g/cm3）或%（m/m）--盐酸（hcl） 1.18～1.19 g/cm3或36%～38%--氢氟酸（hf）1.13 g/cm3或40%--硝酸（hno3）1.39～1.41 g/cm3或65%-68%--硫酸（h2so4）1.84 g/cm3或95%-98%--冰醋酸（ch3cooh）1.049 g/cm3或99.8%--磷酸（h3po4）1.68 g/cm3或85%--氨水（nh3〃h2o） 0.90～0.91 g/cm3或25%-28%在化学分析中，所用酸或氨水，，凡未注明浓度者均指市售的浓酸或氨水。
用体积比表示试剂稀释程度，例如：盐酸（1+2）表示：1份体积的浓盐酸与2份体积的水相混合。
1.1 盐酸 (1+1);（1+2）；（1+11）；（1+5）;(3+97)1.2 硫酸ｈqkqｉ；（1+2）；(1+4);（1+9）1.3 氨水（1+1）；（1+2）1.4 氢氧化钠（naoh）1.5 氢氧化钾（koh）1.6 氯化铵（nh4cl）1.7 氢氧化钾溶液（200g/l）：将200g氢氧化钾溶于水中，加水稀释至1l，贮存于塑料瓶中。
1.8 硝酸银溶液（5g/l）：将5g硝酸银（agno3）溶于水中，加10ml硝酸（hno3），水稀释至1l。
1.9 焦硫酸钾（k2s2o7）：将市售焦硫酸钾在瓷蒸发皿中加热熔化，待气泡停止发生后，冷却、砸碎、贮存于磨口瓶中。
1.10 氯化钡溶液（100g/l）：将100g二水氯化钡（bacl2〃2h2o）溶于水中，加水稀释至1l。
1.11 碳酸铵溶液（100g/l）：将10g碳酸铵[（nh4）2co3]溶于100ml水中。
1.12 edta-铜：按[c（edta）=0.015mol/l]标准滴定溶液与[c（cuso4）=0.015mol/l]硫酸铜标准滴定溶液体积比，准确配制成等浓度的混合溶液。
1.13 ph3的缓冲溶液：将3.2g无水乙酸钠（ch3coona）溶于水中，加120ml冰乙酸（ch3coona）用水稀释至1l，摇匀。
1.14 ph4.3的缓冲溶液：将42.3g无水乙酸钠（ch3coona）溶于水中，加80ml冰乙酸（ch3cooh），用水稀释至1l，摇匀。
1.15 ph10缓冲溶液：将67.5g氯化铵（nh4cl）溶于水中，加570ml氨水，加水稀释至1l，摇匀。
1.16 无水碳酸钠。
1.17 氢氧化钠溶液（10g/l:）将10g氢氧化钠（naoh）溶于水中，加水稀释至1l，贮存于塑料瓶中。
1.18 三乙醇胺[n（ch2ch2oh）3]：（1+2）1.19 酒石酸钾钠溶液（100g/l）：将100g酒石酸钾钠（c4h4kna〃4h2o）溶于水中，稀释至1l。
1.20 氯化钾(kcl)：颗料大时，应研细后使用。
1.21 氟化钾溶液（150g/l）：称取150g氟化钾（kf〃2h2o）溶于水中，稀释至1l，贮存于塑料瓶中。
1.22 氟化钾溶液（20g/l）：称取20g氟化钾（kf〃2h2o）溶于水中，稀释1l，贮于塑料瓶中。
1.23 氯化钾溶液（50g/l）：将50g氯化钾(kcl)溶于水中，用水稀释至1l。
1.24 氯化钾-乙醇（50g/l）：将5g氯化钾(kcl)溶于50ml水中，加入50ml95%（v/v）乙醇（c2h5oh）,混匀。
1.25 氯化锶溶液（锶50g/l）：将152.2g氯化锶（srcl2〃6h2o）溶解于水中，用水稀释至1l，必要时过滤。
1.26 阳离子交换树脂：001×7（旧型号为732）苯乙烯型强酸性阳离子交换树脂（1×12）。
1.27 硝酸铵溶液（20g/l）：将20g硝酸铵溶于水中，加水稀释至1l，摇匀。
1.28 氧化钾（k2o）、氧化钠（na2o）标准溶液1.28.1 氧化钾标准溶液的配制称取0.792g已于130～150℃烘过2h的氯化钾(kcl)，精确至0.0001g，置于烧杯中，加水溶解后，移入1000ml容量瓶中，用水稀释至标线，摇匀。
贮存于塑料瓶中。
此标准溶液每毫升相当于0.5mg氧化钾。
1.28.2 氧化钠标准溶液的配制称取0.943g已于130～150℃烘过2h的氯化钠（nacl），精确至0.0001g，置于烧杯中，加水溶解后，移入1000ml容量瓶中，用水稀释至标线，摇匀。
贮存于塑料瓶中。
此标准溶液每毫升相当于0.5mg氧化钠。
1.28.3 工作曲线的绘制1.28.3.1 用于火焰光度法的工作曲线的绘制吸取按4.37.1配制的每毫升相当于0.5mg氧化钾的标准溶液0、 1.00、2.00、4.00、6.00、8.00、10.00、12.00m和按4.27.2配制的每毫升相当于0.5mg氧化钠的标准溶液0、1.00、2.00、4.00、6.00、8.00、10.00、12.00，以一一对应的顺序，分别放入100ml容量瓶中，用水稀释至标线，摇匀。
使用火焰光度计按仪器使用规程进行测定。
用测得的检流计读数作为相对应的氧化钾和氧化钠含量的函数，绘制工作曲线。
1.29 碳酸钙基准溶液[c（caco3）=0.024mol/l]称取0.6（m1）已于105～110℃烘过2h的碳酸钙（caco3），精确至0.0001g，置于400ml烧杯中，加入约100ml水，盖上表面皿，沿杯口滴加盐酸（1+1）至碳酸钙全部溶解，加热煮沸数分钟将溶液冷至室温，移入250ml容量瓶中，用水稀释至标线，摇匀。
edta标准滴定溶液[c（edta）=0.015mol/l]1.30.1 标准滴定溶液的配制称取5.6g（乙二胺四乙酸二钠盐）置于烧杯中，加约200ml水，加热溶解，过滤，用水稀释至1l。
1.31.2 标准滴定溶液浓度的标定吸取25.00ml碳酸钙基准溶液于400ml烧杯中，加水稀释至约200ml加入适量cmp混合指示剂，在搅拌下加入氢氧化钾溶液到出现绿色荧光后再过量2～3ml，以edta标准滴定溶液滴定至绿色荧光消失并呈现红色。
edta标准滴定溶液的浓度按下式计算：m×25×1000c（edta）=￣────────────250×v4×100.09式中：c（edta）-edta标准滴定溶液的浓度，mol/lv4-滴定时消耗edta标准滴定溶液的体积，mlm1-按4.41配制碳酸钙基准溶液的碳酸钙的质量，g100.09-caco3的摩尔质量，g/mol1.30.3 edta标准滴定溶液对各氧化物滴定度的计算edta标准滴定溶液对三氧化二铁、三氧化二铝、氧化钙、氧化镁的滴定度分别按下式计算：tfe2o3=c（edta）×79.84tal2o3=c（edta）×50.98篇三：迈瑞bs-480全自动生化分析仪标准操作规程迈瑞bs-480全自动生化分析仪标准操作规程1 bs-480全自动生化分析仪标准操作规程（开/关机程序）1.1 开机1.1.1 依次打开总电源、ups电源、分析部主电源、分析部电源、操作部显示器电源、操作部主机电源；1.1.2 开启操作部主机后启动操作软件，在对话框中输入用户名：serviceuser 密码；#bs8a#seu(1) 若只关闭分析部电源保持试剂盘制冷，则要依次打开分析部电源、操作部显示器电源、操作部主机电源；(2) 若使用仪器睡眠功能，则只需在对话框中输入用户名与密码，重新登陆；1.2 分析前准备1.2.1 观察各压力表是否在绿色标线之内；1.2.2 检查去离子水是否足够、废液管道有否堵塞，废液桶是否清空；1.2.3 检查高浓度清洗桶是否有足够高浓度清洗液；1.2.4 确认试剂盘的d1号位置已放置碱清洗液，d2号位置已放置酸清洗液，w号位置已放置去离子水。
1.2.5 检查样本针，确认无污物，无弯折。
如有污物，清洗样本针。
如有弯折，更换样本针。
1.2.6 检查试剂针，确认无污物，无弯折。
如有污物，清洗样本针。
如有弯折，更换样本针。
1.2.7 检查搅拌杆，确认搅拌杆表面无污物，杆无弯折。
如有污物，清洗搅拌杆。
1.3 关机1.3.1 仪器处于"空闲"状态时，可以点击【退出】按钮，按提示选择【关机】或【退出操作软件】进行关机操作。
依次关闭操作部主机电源，操作部显示器电源，分析部电源，分析部主电源，ups电源、总电源。
此时需要取走试剂盘内试剂放到冰箱或冷库保存。
1.3.2 如保留试剂制冷功能，则不需要关闭分析部主电源、ups电源和总电源。
1.3.3 如需切换不同操作者，仪器处于"空闲"状态时，可点击【退出】按钮选择【注销】后重新以新用户登陆。
1.3.4 如需进行休眠功能，仪器处于"空闲"状态时，可点击【退出】按钮选择【休眠】，仪器进入休眠状态。
1.3.5 清理取走样本盘所有标本。
2 bs-480全自动生化分析仪标准操作规程分析参数设置程序2.1 在主界面下选择【应用】→【项目设置】，选择相应位置，点击【设置f1】，在弹出对话框中选择生化项目，点击【确定】，进行参数设置；2.1.1 项目设置；(1) 项目简称：项目的简要名称，由英文字母、数字、下划线、+、-、*、/ 组成。
(2) 项目全称：项目的完整名称，可以为空。
(3) 样本类型：在下拉菜单中选择该项目要测试的样本类型（血清、血浆、尿液、脑脊液、其他）。
(4) 分析方法：在下拉菜单中选择该项目的测定方法（动力学法、固定时间法、终点法）。
(5) 反应方向：下拉菜单选择"上升"、"下降"。
为必须输入内容。
(6) 主波长：下拉菜单选择测试所用的主波长。
为必须输入内容。
(7) 次波长：下拉菜单选择测试所用的次波长，默认为空。
(9) 结果位数：表示报告结果的有效数字，下拉菜单选择测试结果的精度。
为必须输入内容。
(10) 空白时间：表示参与计算的试剂空白的测光点区间，其中，对于单试剂选择范围5-12，对于双试剂选择范围14-49；第一个不能大于第二个输入框的值。
终点法为必需输入内容，速率法与固定时间法可不输入。
注意，其区间不能与反应时间区间交叉或重叠，更不能大于反应时间的起始数据点。
(11) 反应时间：反应时间对于终点法是指开始反应到反应结束的时间跨度。
对于动力学法和固定时间法是指反应稳定到停止对反应监测的时间跨度。