酶切位点表
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GACGCGTC CGACGCGTCG
CCCATGGG CATGCCATGGCATG
CCATATGG CCCATATGGG CGCCATATGGCG GGGTTTCATATGAAACCC GGAATTCCATATGGAATTCC GGGAATTCCATATGGAATTCCC
GGCTAGCC CGGCTAGCCG CTAGCTAGCTAG
GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA
CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA
CGGATCCG CGGGATCCCG CGCGGATCCGCG
CAGATCTG GAAGATCTTC GGAAGATCTTCC
GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA
50
>90
>90
>90
>90
>90
>90
>90
>90
>90
>90
TTGCGGCCGCAA
>90
>90
Hind III Kpn I Mlu I Nco I Nde I
Nhe I 酶 Not I
Nsi I Pac I Pme I
CAAGCTTG CCAAGCTTGG CCCAAGCTTGGG
GGGTACCC GGGGTACCCC CGGGGTACCCCG
0
50
AGCTTTGTTTAAACGGCGCGCCGG
75
>90
Pst I
GCTGCAGC
0
0
TGCACTGCAGTGCA
10
10
AACTGCAGAACCAATGCATTGG
>90
>90
AAAACTGCAGCCAATGCATTGGAA
>90
>90
CTGCAGAACCAATGCATTGGATGCAT
CCTCGAGG CCCTCGAGGG CCGCTCGAGCGG
CCCCGGGG CCCCCGGGGG CCCCCCGGGGGG TCCCCCCGGGGGGA
0
0
>90
>90
75
>90
75
>90
0
0
10
25
10
75
0
0
25
75
50
>90
>90
>90
10
>90
10
>90
0
50
0
50
Sph I
GGCATGCC CATGCATGCATG ACATGCATGCATGT
0
0
0
25
10
50
Stu I
AAGGCCTT
>90
>90
GAAGGCCTTC
>90
>90
AAAAGGCCTTTT
>90
>90
Xba I Xho I Xma I
CTCTAGAG GCTCTAGAGC TGCTCTAGAGCA CTAGTCTAGACTAG
GGGGCCCC AGCGGCCGCT
切割率%
2 hr
20 hr
0
0
0
0
0
0
0
0
>90
>90
>90
>90
>90
>90
>90
>90
>90
>90
50
>90
>90
>90
>90
>90
10
25
>90
>90
>90
>90
0
0
75
>90
25
>90
0
0
0
0
50
>90
0
10
0
0
25
50
25
>90
0
0
0
0
>90
>90
50
0
0
Pvu I
CCGATCGG ATCGATCGAT TCGCGATCGCGA
0
0
10
25
0
10
Sac I
CGAGCTCG
10
10
Sac II
GCCGCGGC TCCCCGCGGGGA
0
0
50
>90
Sal I
GTCGACGTCAAAAGGCCATAGCGGCCGC
0
0
GCGTCGACGTCTTGGCCATAGCGGCCGCG 10
GGGT(A/T)ACCC
AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT
CATCGATG GATCGATC CCATCGATGG CCCATCGATGGG
GGAATTCC CGGAATTCCG CCGGAATTCCGG
50
G
10
75
ACGCGTCGACGTCGGCCATAGCGGCCGCG
GAA
Sca I
GAGTACTC AAAAGTACTTTT
10
25
75
75
Sma I
CCCGGG CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA
0
10
0
10
10
50
>90
>90
Spe I
GACTAGTC GGACTAGTCC CGGACTAGTCCG CTAGACTAGTCTAG
寡核苷酸序列
TTGCGGCCGCAA ATTTGCGGCCGCTTTA AAATATGCGGCCGCTATAAA ATAAGAATGCGGCCGCTAAACTAT AAGGAAAAAAGCGGCCGCAAAAGGAAAA
TGCATGCATGCA CCAATGCATTGGTTCTGCAGTT
TTAATTAA GTTAATTAAC CCTTAATTAAGG
GTTTAAAC GGTTTAAACC GGGTTTAAACCC
0
0
0
0
10
75
0
0
>90
>90
>90
>90
0
0
25
50
0
Fra Baidu bibliotek
0
50
75
0
0
0
0
0
0
0
0
75
>90
75
>90
0
0
10
25
10
50
切割率%
2 hr
20 hr
0
0
10
10
10
10
25
90
25
>90
10
>90
>90
>90
0
0
0
25
0
>90
0
0
0
25
酶 Acc I Afl III Asc I Ava I BamH I Bgl II BssH II BstE II BstX I Cla I
EcoR I Hae III
寡核苷酸序列
GGTCGACC CGGTCGACCG CCGGTCGACCGG
CACATGTG CCACATGTGG CCCACATGTGGG
CCCATGGG CATGCCATGGCATG
CCATATGG CCCATATGGG CGCCATATGGCG GGGTTTCATATGAAACCC GGAATTCCATATGGAATTCC GGGAATTCCATATGGAATTCCC
GGCTAGCC CGGCTAGCCG CTAGCTAGCTAG
GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA
CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA
CGGATCCG CGGGATCCCG CGCGGATCCGCG
CAGATCTG GAAGATCTTC GGAAGATCTTCC
GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA
50
>90
>90
>90
>90
>90
>90
>90
>90
>90
>90
TTGCGGCCGCAA
>90
>90
Hind III Kpn I Mlu I Nco I Nde I
Nhe I 酶 Not I
Nsi I Pac I Pme I
CAAGCTTG CCAAGCTTGG CCCAAGCTTGGG
GGGTACCC GGGGTACCCC CGGGGTACCCCG
0
50
AGCTTTGTTTAAACGGCGCGCCGG
75
>90
Pst I
GCTGCAGC
0
0
TGCACTGCAGTGCA
10
10
AACTGCAGAACCAATGCATTGG
>90
>90
AAAACTGCAGCCAATGCATTGGAA
>90
>90
CTGCAGAACCAATGCATTGGATGCAT
CCTCGAGG CCCTCGAGGG CCGCTCGAGCGG
CCCCGGGG CCCCCGGGGG CCCCCCGGGGGG TCCCCCCGGGGGGA
0
0
>90
>90
75
>90
75
>90
0
0
10
25
10
75
0
0
25
75
50
>90
>90
>90
10
>90
10
>90
0
50
0
50
Sph I
GGCATGCC CATGCATGCATG ACATGCATGCATGT
0
0
0
25
10
50
Stu I
AAGGCCTT
>90
>90
GAAGGCCTTC
>90
>90
AAAAGGCCTTTT
>90
>90
Xba I Xho I Xma I
CTCTAGAG GCTCTAGAGC TGCTCTAGAGCA CTAGTCTAGACTAG
GGGGCCCC AGCGGCCGCT
切割率%
2 hr
20 hr
0
0
0
0
0
0
0
0
>90
>90
>90
>90
>90
>90
>90
>90
>90
>90
50
>90
>90
>90
>90
>90
10
25
>90
>90
>90
>90
0
0
75
>90
25
>90
0
0
0
0
50
>90
0
10
0
0
25
50
25
>90
0
0
0
0
>90
>90
50
0
0
Pvu I
CCGATCGG ATCGATCGAT TCGCGATCGCGA
0
0
10
25
0
10
Sac I
CGAGCTCG
10
10
Sac II
GCCGCGGC TCCCCGCGGGGA
0
0
50
>90
Sal I
GTCGACGTCAAAAGGCCATAGCGGCCGC
0
0
GCGTCGACGTCTTGGCCATAGCGGCCGCG 10
GGGT(A/T)ACCC
AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT
CATCGATG GATCGATC CCATCGATGG CCCATCGATGGG
GGAATTCC CGGAATTCCG CCGGAATTCCGG
50
G
10
75
ACGCGTCGACGTCGGCCATAGCGGCCGCG
GAA
Sca I
GAGTACTC AAAAGTACTTTT
10
25
75
75
Sma I
CCCGGG CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA
0
10
0
10
10
50
>90
>90
Spe I
GACTAGTC GGACTAGTCC CGGACTAGTCCG CTAGACTAGTCTAG
寡核苷酸序列
TTGCGGCCGCAA ATTTGCGGCCGCTTTA AAATATGCGGCCGCTATAAA ATAAGAATGCGGCCGCTAAACTAT AAGGAAAAAAGCGGCCGCAAAAGGAAAA
TGCATGCATGCA CCAATGCATTGGTTCTGCAGTT
TTAATTAA GTTAATTAAC CCTTAATTAAGG
GTTTAAAC GGTTTAAACC GGGTTTAAACCC
0
0
0
0
10
75
0
0
>90
>90
>90
>90
0
0
25
50
0
Fra Baidu bibliotek
0
50
75
0
0
0
0
0
0
0
0
75
>90
75
>90
0
0
10
25
10
50
切割率%
2 hr
20 hr
0
0
10
10
10
10
25
90
25
>90
10
>90
>90
>90
0
0
0
25
0
>90
0
0
0
25
酶 Acc I Afl III Asc I Ava I BamH I Bgl II BssH II BstE II BstX I Cla I
EcoR I Hae III
寡核苷酸序列
GGTCGACC CGGTCGACCG CCGGTCGACCGG
CACATGTG CCACATGTGG CCCACATGTGGG