人转移因子(TF)ELISA试剂盒说明书,进口ELISA试剂盒
Human TNF-a ELISA Kit 操作手册说明书
Human TNF‑a ELISA KitCatalog Number KHC3011 (96 tests), KHC3012 (2 × 96 tests), and KHC3011C (5 × 96 tests)Pub. No. MAN0003931 Rev.5.0 (32)CAUTION! This kit contains materials with small quantities of sodium azide and Proclin™ 300. Sodium azide reacts with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a large volume of water to prevent azide accumulation. In case of contact, rinse affected area with plenty of water. Proclin™ 300 is toxic, corrosive, and a skin irritant. Avoid ingestion and contact with eyes, skin and mucous membranes. In case of contact, rinse affected area with plenty of water. Observe all federal, state, and local regulations for disposal.Note: For safety and biohazard guidelines, see the “Safety” appendix in the ELISA Technical Guide (Pub. no. MAN0006706). Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves.Product descriptionThe Invitrogen™ Human TNF-a ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA). This assay is designed to detect and quantify the level of human TNF-a in human serum, plasma, buffered solutions, or cell culture medium. The assay recognizes both natural and recombinant human TNF-a.Human tumor necrosis factor alpha (Hu TNF-a), also called cachectin, is a 157 AA non-glycosylated polypeptide cytokine produced by activated macrophages. Lipopolysaccharide (LPS) is a potent stimulus for TNF-a production in macrophages, and TNF-a is an important mediator of the in vivo effects of LPS. The biological activities of TNF-a may be classified as:- Immunomodulating and proinflammatory: TNF-a regulates the production of antibodies by B cells and stimulates cytotoxic T cells.- Metabolic: TNF-a strongly inhibits lipoprotein lipase and adipocyte gene expression.Contents and storageUpon receipt, store the kit at 2°C to 8°C.Materials required but not supplied•Distilled or deionized water•Microtiter plate reader with software capable of measurement at or near 450 nm•Plate washer–automated or manual (squirt bottle, manifolddispenser, or equivalent)•Calibrated adjustable precision pipettes and glass or plastic tubes for diluting solutions Before you beginIMPORTANT! Reagents are lot-specific. Do not mix or interchange different reagent lots from various kit lots.•Review the Procedural guidelines and Plate washing directions in the ELISA Technical Guide available at .•Allow reagents to reach room temperature before use. Mix toredissolve any precipitated salts.Prepare 1X Wash Buffer1.Dilute 16 mL of Wash Buffer Concentrate (25X) with 384 mL ofdeionized or distilled water. Label as 1X Wash Buffer.2.Store the concentrate and 1X Wash Buffer in the refrigerator. Usethe diluted buffer within 25 days.Sample preparation guidelines•Refer to the ELISA Technical Guide at for detailed sample preparation procedures.•Collect samples in pyrogen/endotoxin-free tubes.•Freeze samples after collection if samples will not be tested immediately. Avoid multiple freeze-thaw cycles of frozen samples. Thaw completely and mix well (do not vortex) prior to analysis.•Avoid the use of hemolyzed or lipemic sera. If large amounts of particulate matter are present in the sample, centrifuge or filter sample prior to analysis.Pre-dilute samplesSample concentrations should be within the range of the standard curve. Because conditions may vary, each investigator should determine the optimal dilution for each application.Perform sample dilutions with Standard Diluent Buffer (serum/plasma) or with the corresponding cell culture medium (cell culture supernatant). Dilute standardsNote: Use glass or plastic tubes for diluting standards.Note: This assay has been calibrated against the International Standard preparation (87/650) for Hu TNF-a (NIBSC, Hertforshire, UK, EN6 3QG). One microgram equals 40,000 International Units.1.Reconstitute Hu TNF-a Standard to 2000 pg/mL with Standard Dilution Buffer. Refer to the standard vial label for instructions. Swirl or mixgently and allow the contents to sit for 10 minutes to ensure complete reconstitution. Label as 2000 pg/mL human TNF-a. Use the standard within 1 hour of reconstitution.2.Add 300 µL Reconstituted Standard to one tube containing 300 µL Standard Diluent Buffer and mix. Label as 1000 pg/mL human TNF-a.3.Add 300 µL Standard Diluent Buffer to each of 7 tubes labeled as follows: 500, 250, 125, 62.5, 31.2, 15.6, and 0 pg/mL human TNF-a.4.Make serial dilutions of the standard as shown in the following dilution diagram. Mix thoroughly between steps.5.Discard all remaining reconstituted and diluted standards after completing assay. Return the Standard Diluent Buffer to the refrigerator.DiluentVolumeStd5Std4Std3Std2Std1Std6Std7Std0300 μL250 pg/mL1000 pg/mL500 pg/mL15.6 pg/mL31.2 pg/mL62.5 pg/mL125 pg/mL2000 pg/mL300 μL300 μL300 μL300 μL300 μL300 μL300 μL300 μL0 pg/mLPrepare 1X Streptavidin‑HRP solutionNote: Prepare 1X Streptavidin-HRP within 15 minutes of usage.The Streptavidin-HRP (100X) is in 50% glycerol, which is viscous. To ensure accurate dilution:1.For each 8-well strip used in the assay, pipet 10 µL Streptavidin-HRP (100X) solution, wipe the pipette tip with clean absorbent paper toremove any excess solution, and dispense the solution into a tube containing 1 mL of Streptavidin-HRP Diluent. Mix thoroughly.2.Return the unused Streptavidin-HRP (100X) solution to the refrigerator.Perform ELISA (Total assay time: 4 hours)IMPORTANT! Perform a standard curve with each assay.•Allow all components to reach room temperature before use. Mix all liquid reagents prior to use.•Determine the number of 8-well strips required for the assay. Insert the strips in the frames for use. Re-bag any unused strips and frames, andstore at 2°C to 8°C for future use.a.Add 50 µL of Incubation Buffer to wells for serum or plasma samples, standards, or controls; or 50 µLof Standard Diluent Buffer to the wells for cell culture samples. Leave the wells for chromogen blanksempty.b.Add 100 µL of standards, controls, or samples (see “Pre-dilute samples” on page 2) to the appropriatewells. Leave the wells for chromogen blanks empty.c.Tap the side of the plate to mix. Cover the plate with a plate cover and incubate for 2 hours at roomtemperature.d.Thoroughly aspirate the solution and wash wells 4 times with 1X Wash Buffer.1Bind antigenCulture media +Standard DiluentBufferStandards/Serum/Plasma/Controls +Incubation Buffera.Add 100 µL Hu TNF-a Biotin Conjugate solution into each well except the chromogen blanks.2Add Biotin ConjugateAdd Streptavidin‑HRPAdd Stabilized ChromogenAdd Stop Solution1.Read the absorbance at 450 nm. Read the plate within 2 hours after adding the Stop Solution.e curve-fitting software to generate the standard curve. A four parameter algorithm provides the best standard curve fit. Optimally, thebackground absorbance may be subtracted from all data points, including standards, unknowns and controls, prior to plotting.3.Read the concentrations for unknown samples and controls from the standard curve. Multiply value(s) obtained for sample(s) by theappropriate factor to correct for the sample dilution.Note: Dilute samples producing signals greater than the upper limit of the standard curve in Standard Diluent Buffer (serum/plasma) or with the corresponding cell culture medium (cell culture supernatant) and reanalyze. Multiply the concentration by the appropriate dilution factor. Performance characteristicsStandard curve exampleThe following data were obtained for the various standards over therange of 0−1000 pg/mL human TNF-a.Inter-assay precisionSamples were assayed 18 times in multiple assays to determineprecision between assays.Intra-assay precisionSamples of known human TNF-a concentration were assayed inreplicates of 16 to determine precision within an assay.Expected valuesHuman PBMCs or whole blood were stimulated from 4 to 72 hours with lipopolysaccharide (LPS), phytohaemagglutinin (PHA), or ionomycin and phorbol myristate acetate (PMA) then evaluated for the presence of human TNF-a in this assay. Whole blood samples were pre-diluted 10-fold for the assay.Linearity of dilutionHuman serum containing 806 pg/mL of measured human TNF-a was serially diluted in Standard Diluent Buffer over the range of the assay. Linear regression analysis of samples versus the expected concentration yielded a correlation coefficient of 0.99.RecoveryThe average recovery of Human TNF-a ELISA Kit added to a variety of samples is listed in the following table.SensitivityThe analytical sensitivity of ths assay is 1.7 pg/mL human TNF-a. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times. SpecificityBuffered solutions of The following panel of substances were assayed at 50 ng/mL and were found to have no cross-reactivity: human IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IFN-a, IFN-b, IFN-g, GM-CSF, OSM,MIP-1a, MIP-1b, LIF, MCP-1, G-CSF, TGF-b, RANTES; swine TNF-a; rat TNF-a; mouse TNF-a.Limited product warrantyLife Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the Life Technologies' General Terms and Conditions of Sale at /us/en/home/global/terms-and-conditions.html. If you have any questions, please contact Life Technologies at /support.Product label explanation of symbols and warningsManufacturer's address: Bender MedSystems GmbH | Campus Vienna Biocenter 2 | 1030 Vienna, AustriaThe information in this guide is subject to change without notice.DISCLAIMERTO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept the terms and conditions of all applicable Limited Use Label Licenses.©2019 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified./support | /askaquestion。
人血管内皮生长因子 ELISA 试剂盒说明书
产品说明书人血管内皮生长因子ELISA试剂盒（Human VEGF ELISA KIT）产品货号：H6139S, H6139M, H6139L产品规格：24T, 48T, 96T产品内容：注：终止液和显色剂具有腐蚀性，一旦接触到液体，请尽快用大量清水冲洗。
储存条件4℃避光保存，有效期见外包装。
开封后，保存温度详见说明书。
产品介绍Human VEGF ELISA Kit (Human Vascular Endothelial Cell Growth Factor Enzyme-Linked ImmunoSorbent Assay Kit) ，即人血管内皮生长因子ELISA试剂盒，可以定量检测人血清、血浆或细胞培养上清液等样本中的天然和重组VEGF浓度。
人VEGF是由两个相同亚基以二硫键交联组成的二聚体糖蛋白，基因定位于第6号染色体长臂，由8个外显子和7个内含子交替构成，约14 kb。
由于mRNA剪接方式不同，产生了单链分别含121，145，165，183，189和206个氨基酸的不同变异体。
VEGF 121分子量约为34-46 kDa，是可溶性分泌型蛋白质，以游离状态存在，不能与肝素结合；VEGF 165分子量为45 kDa，30-50%以可溶性形式分泌于细胞外基质，其余部分与细胞膜、基底膜或细胞外基质含有肝素的糖蛋白结合。
VEGF 145存在于胎盘细胞和女性生殖道肿瘤细胞。
VEGF 189及VEGF 206分泌后完全结合于细胞膜或基底膜含有肝素的糖蛋白上。
骨骼肌，心肌，肝细胞，成骨细胞，中性粒细胞，巨噬细胞，角质形成细胞，血小板，褐色脂肪组织，CD34+细胞，星形细胞，神经元和内皮细胞等多种细胞和组织均可产生VEGF。
血清和血浆样本均可检测到VEGF，由于血小板可释放VEGF，因此血清中VEGF含量较高。
VEGF是一种特异作用于血管内皮细胞的多功能细胞因子，可增加血管通透性、促进血管形成、引起细胞外基质成分改变等。
人脂肪炎症因子(Apelin)ELISA检测试剂盒简介说明
人脂肪炎症因子(Apelin)ELISA检测试剂盒简介说明人脂肪炎症因子(Apelin)ELISA检测试剂盒说明书检测原理试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）。
往预先包被脂肪炎症因子（Apelin /APLN）抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并彻di洗涤。
用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。
颜色的深浅和样品中的脂肪炎症因子（Apelin /APLN）呈正相关。
用酶标仪在450nm 波长下测定吸光度（OD 值），计算样品浓度。
样品收集、处理及保存方法1. 血清：使用不含热原和内毒素的试管，操作过程中避免任何细胞刺激，收集血液后，3000转离心10分钟将血清和红细胞迅速小心地分离。
2. 血浆：EDTA、柠檬酸盐或肝素抗凝。
3000转离心30分钟取上清。
3. 细胞上清液：3000转离心10分钟去除颗粒和聚合物。
4. 组织匀浆：将组织加入适量生理盐水捣碎。
3000转离心10分钟取上清。
5. 保存：如果样本收集后不及时检测，请按一次用量分装，冻存于20℃，避免反复冻融，在室温下解冻并确保样品均匀地充分解冻。
自备物品1. 酶标仪（450nm）2. 高精度加样器及枪头：0.510uL、220uL、20200uL、2001000uL3. 37℃恒温箱操作注意事项试剂盒保存在28℃，使用前室温平衡20分钟。
从冰箱取出的浓缩洗涤液会有结晶，这属于正常现象，水浴加热使结晶wan全溶解后再使用。
实验中不用的板条应立即放回自封袋中，密封（低温干燥）保存。
浓度为0的S0号标准品即可视为阴性对照或者空白；按照说明书操作时样本已经稀释5倍，最终结果乘以5才是样本实际浓度。
严格按照说明书中标明的时间、加液量及顺序进行温育操作。
所有液体组分使用前充分摇匀。
试剂盒组成名称 96孔配置 48孔配置备注微孔酶标板 12孔×8条 12孔×4条无标准品 0.3mL*6管 0.3mL*6管无样本稀释液 6mL 3mL 无检测抗体HRP 10mL 5mL 无20×洗涤缓冲液 25mL 15mL 按说明书进行稀释底物A 6mL 3mL 无底物B 6mL 3mL 无终止液 6mL 3mL 无封板膜 2张 2张无说明书 1份 1份无自封袋 1个 1个无注：标准品（S0S5）浓度依次为：0、0.5、1、2、4、8 ng/mL试剂的准备20×洗涤缓冲液的稀释：蒸馏水按1：20稀释，即1份的20×洗涤缓冲液加19份的蒸馏水。
biosharp 人IL-2 ELISA 试剂盒使用说明书
一、产品简介 ������������������� ���������二、检测原理 ������������������� ���������三、试剂盒组分 ������������������� ���������四、储存条件 ������������������ ����������五、注意事项 ������������������ ����������六、其它实验材料 ������������������� ��������七、使用说明 �������������������� �������7.1、样品收集、处理及保存方法 �������������������� � 7.2、试剂准备 �������������������� ������� 7.3、操作步骤 �������������������� ������� 7.4、操作流程图 �������������������� ������� 7.5、操作要点提示 �������������������� ���7.6、结果判断 �������������������� �������八、常见问题分析及解决 ���������� ������������� 2 2 3 3 3 4 4 4 4 5 6 6 6 8目 录全国统一热线：400-600-4213 Elisa试剂盒使用说明书本试剂盒用于定量检测人血清、血浆或细胞培养上清液等样本中天然和重组IL-2浓度。
使用前请仔细阅读说明书并检查试剂组分，如有任何疑问请与北京兰杰柯科技有限公司或经销商联系，公司将为您提供强有力的技术支持。
仅供研究，不用于临床诊断。
二、检测原理本实验采用双抗体夹心ELISA，用抗人IL-2单克隆抗体预包被酶标板，加入适度稀释的样本和标准品，其中IL-2会与其单抗结合，洗去游离成分；加入生物素化的抗人IL-2抗体，抗人IL-2抗体与结合在单抗上的人IL-2结合而形成免疫复合物，洗去游离的成分；加入辣根过氧化物酶标记的亲合素，生物素与亲合素特异性结合，洗去未结合的酶结合物；加入显色剂，若反应孔中有IL-2，辣根过氧化物酶会使无色的显色剂现蓝色；加终止液变黄。
人趋化因子elisa试剂盒使用说明书
人趋化因子（FK）elisa试剂盒使用说明书Elisa kit规格：48孔配置/96孔配置标准品稀释液：1.5ml X 1瓶酶标试剂：3 ml X 1 瓶（48）/6 ml X 1 瓶（96）【人趋化因子（FK）elisa试剂盒elisa试剂盒】本试剂仅供研究使用计算以标准物的浓度为横坐标，0D值为纵坐标，在坐标纸上绘出标准曲线，根据样品的0D值由标准曲线查出相应的浓度；再乘以稀释倍数；或用标准物的浓度与OD值计算出标准曲线的直线回归方程式，将样品的OD值代入方程式，计算出样品浓度，再乘以稀释倍数，即为样品的实际浓度。
试剂盒组成：封板膜:2片（48）/2片（96）说明书:1份密封袋:1个标准品：2700ng/L 0.5ml X 1 瓶0.5ml X 1 瓶2-8C保存酶标包被板：1X 481X 962-8 C保存样品稀释液：3ml X 1 瓶 6 ml X 1 瓶2-8 C保存显色剂A液:3ml X 1 瓶 6 ml X 1 瓶2-8 C保存显色剂B液:3ml X 1 瓶 6 ml X 1 瓶2-8 C保存终止液：3ml X 1 瓶6ml X 1 瓶2-8 C保存浓缩洗涤液：（20ml X 20倍）X 1瓶（20ml X 30倍）X 1瓶2-8C保存实验原理：本试剂盒应用双抗体夹心法测定标本中人趋化因子（FK）elisa试剂盒水平。
用纯化的人趋化因子（FK）elisa试剂盒抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入趋化因子（FK）elisa试剂盒，再与HRP标记的趋化因子（FK）elisa试剂盒抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。
TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。
颜色的深浅和样品中的趋化因子（FK）elisa试剂盒呈正相关。
用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中人趋化因子（FK）elisa 试剂盒浓度。
人(Human)轴突生长诱向因子1(Ntn1)ELISA试剂盒说明书
本试剂盒只能用于科学研究，不得用于医学诊断人（Human）轴突生长诱向因子1（Ntn1）ELISA检测试剂盒使用说明书检测原理试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）。
往预先包被轴突生长诱向因子1（Ntn1）抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并彻底洗涤。
用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。
颜色的深浅和样品中的轴突生长诱向因子1（Ntn1）呈正相关。
用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
样品收集、处理及保存方法1.血清：使用不含热原和内毒素的试管，操作过程中避免任何细胞刺激，收集血液后，3000转离心10分钟将血清和红细胞迅速小心地分离。
2.血浆：EDTA、柠檬酸盐或肝素抗凝。
3000转离心30分钟取上清。
3.细胞上清液：3000转离心10分钟去除颗粒和聚合物。
4.组织匀浆：将组织加入适量生理盐水捣碎。
3000转离心10分钟取上清。
5.保存：如果样本收集后不及时检测，请按一次用量分装，冻存于-20℃，避免反复冻融，在室温下解冻并确保样品均匀地充分解冻。
自备物品1.酶标仪（450nm）2.高精度加样器及枪头：0.5-10uL、2-20uL、20-200uL、200-1000uL3.37℃恒温箱操作注意事项1.试剂盒保存在2-8℃，使用前室温平衡20分钟。
从冰箱取出的浓缩洗涤液会有结晶，这属于正常现象，水浴加热使结晶完全溶解后再使用。
2.实验中不用的板条应立即放回自封袋中，密封（低温干燥）保存。
3.浓度为0的S0号标准品即可视为阴性对照或者空白；按照说明书操作时样本已经稀释5倍，最终结果乘以5才是样本实际浓度。
4.严格按照说明书中标明的时间、加液量及顺序进行温育操作。
5.所有液体组分使用前充分摇匀。
试剂盒组成名称96孔配置48孔配置备注微孔酶标板12孔×8条12孔×4条无标准品0.3mL*6管0.3mL*6管无样本稀释液6mL3mL无检测抗体-HRP10mL5mL无20×洗涤缓冲液25mL15mL按说明书进行稀释底物A6mL3mL无底物B6mL3mL无终止液6mL3mL无封板膜2张2张无说明书1份1份无自封袋1个1个无注：标准品（S0-S5）浓度依次为：0、5、10、20、40、80pg/mL试剂的准备20×洗涤缓冲液的稀释：蒸馏水按1：20稀释，即1份的20×洗涤缓冲液加19份的蒸馏水。
人恶性肿瘤特异性生长因子(TSGF)ELISA试剂盒说明每
人恶性肿瘤特异性生长因子（TSGF）ELISA试剂盒说明：每次实验的标准曲线一般设7个不同浓度，加上空白孔，标准曲线一共需要8个孔。
因此，一个48T 试剂盒zui多可以测定40个样本（不做重复且一次用完），一个96T试剂盒zui 多可以测定88个样本（不做重复且一次用完）。
可以根据实际样本数量和实验计划选择合适的产品规格。
操作注意事项：
1.实验中不用的板条应立即放回包装袋中，密封保存，以免变质。
2.试剂应按标签说明书储存，使用前恢复到室温。
稀稀过后的标准品应丢弃，不可保存。
3.使用一次性的吸头以免交叉污染，吸取终止液和底物A、B液时，避免使用带金属部分的加样器。
4.不用的其它试剂应包装好或盖好。
不同批号的试剂不要混用。
保质前使用。
5.洗涤酶标板时应充分拍干，不要将吸水纸直接放入酶标反应孔中吸水。
6.使用干净的塑料容器配置洗涤液。
使用前充分混匀试剂盒里的各种成份及样品。
7.加入试剂的顺序应一致，以保证所有反应板孔温育的时间一样。
8.按照说明书中标明的时间、加液的量及顺序进行温育操作。
9.底物A应挥发，避免长时间打开盖子。
底物B对光敏感，避免长时间暴露于光下。
避免用手接触，有毒。
实验完成后应立即读取OD值。
我司ELISA试剂盒五大优点：
一、高效、灵敏、特异的抗体；
二、稳定的重复性和可靠性；
三、吸附性能好，空白值低，孔底透明度高的固相载体；
四、适用血清、血浆、组织匀浆液、细胞培养上清液、尿液等等多种标本类型；
五、zui大限度的节省实验经费。
人可溶性转铁蛋白受体(sTfR)酶联免疫(ELISA)分析试剂盒使用说明书
人可溶性转铁蛋白受体（sTfR）酶联免疫(ELISA)分析试剂盒使用说明书樊克生物专业供应本试剂盒仅供研究使用。
检测范围：48T 25 ng/L -800 ng/L使用目的：本试剂盒用于测定人血清、血浆及相关液体样本可溶性转铁蛋白受体（sTfR）含量。
实验原理本试剂盒应用双抗体夹心法测定标本中人可溶性转铁蛋白受体（sTfR）水平。
用纯化的人可溶性转铁蛋白受体（sTfR）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入可溶性转铁蛋白受体（sTfR），再与HRP标记的可溶性转铁蛋白受体（sTfR）抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。
TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。
颜色的深浅和样品中的可溶性转铁蛋白受体（sTfR）呈正相关。
用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中人可溶性转铁蛋白受体（sTfR）浓度。
试剂盒组成1 20倍浓缩洗涤液20ml×1瓶7 终止液3ml×1瓶2 酶标试剂3ml×1瓶 8 标准品（1600ng/L）0.5ml×1瓶3 酶标包被板12孔×4条9 标准品稀释液1.5ml×1瓶4 样品稀释液3ml×1瓶 10 说明书1份5 显色剂A液3ml×1瓶 11 封板膜2张6 显色剂B液3ml×1/瓶12 密封袋1个标本要求1．标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。
若不能马上进行试验，可将标本放于-20℃保存，但应避免反复冻融2．不能检测含NaN3的样品，因NaN3抑制辣根过氧化物酶的（HRP）活性。
操作步骤1. 标准品的稀释：本试剂盒提供原倍标准品一支，用户可按照下列图表在小试管中进行稀释。
800 ng/L 5号标准品150μl的原倍标准品加入150μl标准品稀释液400 ng/L 4号标准品 150μl的5号标准品加入150μl标准品稀释液200 ng/L l 3号标准品150μl的4号标准品加入150μl标准品稀释液100 ng/L 2号标准品150μl的3号标准品加入150μl标准品稀释液50 ng/L 1号标准品150μl的2号标准品加入150μl标准品稀释液2. 加样：分别设空白孔（空白对照孔不加样品及酶标试剂，其余各步操作相同）、标准孔、待测样品孔。
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人转移因子(TF)ELISA试剂盒说明书，进口ELISA试剂盒
人转移因子(TF)ELISA试剂盒说明书，进口ELISA试剂盒
供应商：上海乔羽生物有限公司
ELISA试剂盒规格：
(1) 规格：96T 可以测90个样，5个标准孔，1个空白孔
(2) 规格：48T 可以测42个样，5个标准孔，1个空白孔
人转移因子(TF)ELISA试剂盒说明书，进口ELISA试剂盒Operational matters needing
attention:
The reagent should label storage instructions, return to room temperature before use. After
thinning of the standard products should be discarded, can not be saved.
No - in the experiment of the plate should be immediately put back the bag, sealed to prevent
spoilage.
- no other reagents should be packaged or covered. Do not mix different batches of reagents.
Before use.
] using disposable suction head to avoid cross contamination and draw the termination of the
liquid and the substrate of a and B liquid, avoid using a portion of the metal sample adding device.
Plastic container configuration - use clean washing liquid. All samples of ingredients and mix well
before use in the kit.
The A substrate should be volatile, avoid long time to open the lid. Substrate B is sensitive to light
and avoid prolonged exposure to light. Avoid hand contact, toxic. After completion of the
experiment should be immediately read od.
The addition of reagents should be consistent in order to ensure that all reaction plate hole
incubation time.
The temperature in accordance with the amount of liquid and the sequence of instructions
indicated time, sterile operation.
人转移因子(TF)ELISA试剂盒说明书，进口ELISA试剂盒试剂盒组成：
封口膜：2（48）/ 2片（96）
规格：1份
密封袋：1
标准：2700ng／L 0.5×0.5ml×1瓶1瓶存放在2-8
ELISA包被板：1 x 48 x 96 1 2-8存储在
样品稀释：3ml×1毫升×1瓶6瓶存放在2-8
显色剂：液3ml×1毫升×6瓶1瓶存放在2-8
显色剂B液3ml×1毫升×6瓶1瓶存放在2-8
终止液：3ml×6ml×1瓶1瓶存放在2-8
浓缩洗衣液：（2×20）×1瓶（20ml×30）×1瓶存放在2-8
elisa试剂盒价格，elisa试剂盒说明书，elisa检测试剂盒
人转移因子(TF)ELISA试剂盒说明书，进口ELISA试剂盒Operation precautions:
The reagent should label storage instructions, return to room temperature before
use. The standard should be discarded after thinning, can not be saved.
No - in the experiment of the plate should be immediately put back the bag, sealed
to prevent spoilage.
- no other reagents should be packaged or covered. Do not mix different batches of
reagents. Before use.
] using disposable suction head to avoid cross contamination and draw the termination
of the liquid and the substrate of a and B liquid, avoid using a portion of the metal
sample adding device.
Plastic container configuration - use clean washing liquid. All samples of
ingredients and mix well before use in the kit.
The A substrate should be volatile, avoid long time to open the lid. The B substrate
is sensitive to light, avoid long time exposure to light. Avoid hand contact, toxic.
After completion of the experiment should be immediately read od.
The addition of reagents should be consistent in order to ensure that all reaction
plate hole incubation time.
The temperature in accordance with the amount of liquid and the sequence of
instructions indicated time, sterile operation.
人转移因子(TF)ELISA试剂盒说明书，进口ELISA试剂盒上海乔羽生物有限公司专业的提
供elisa试剂盒，进口/国产elisa试剂盒，elisa试剂盒价格 ，elisa试剂盒说明书，专业
elisa试剂盒说明书，欢迎您的来电！
人转移因子(TF)ELISA试剂盒说明书，进口ELISA试剂盒Kit performance
1 sensitivity: the minimum concentration is less than 1 standard. Linearity of
dilution. Sample linear regression and the expected concentration correlation
coefficient R value is 0.990.
2 specificity: no reaction with other cytokines.
3 repeatability: the variation coefficient of the plate and the plate are all less than
10%.
Result judgment and analysis
1, the instrument value: Yu Bo 450nm ELISA od read the hole on the instrument
2, to the OD value as the ordinate, y, corresponding HLA-B27 standard
concentration as the abscissa, x, do the corresponding curve, HLA-B27 content in
the sample can be according to its OD value by standard curve conversion out
corresponding concentration.
3, value range: 0-8.0IU/ml
4, sensitivity: 0.01 IU/ml
人转移因子(TF)ELISA试剂盒说明书，进口ELISA试剂盒
友情提醒：公司经营的产品均为科研实验用，不可用于临床应用。