Seahorse Bioscience XFp Analyzer XFe96 XFe24 Seahorsebio 细胞外流量分析仪
海馬生物能量測定儀 XF24 實驗手冊
可以注意到，設定畫面中分為數個獨立的子頁 面，每個頁面分別用於紀錄實驗中各類參數的細 節，這些資訊用意在協助研究人員追蹤實驗的流 程，除了protocol此項之外其餘的內容均不會直 接影響實驗的結果，不過強烈建議使用者確實的 紀錄實驗的細節，以便後續的追蹤。
已編寫的流程一覽，執行指令時會由上向下執行，每當使用者新增指令 的時候，指令會新增在圖中藍色光棒的上方。
OCR (pmol/min) 25-50 50-100 100-200 200-400 400-800 800+
Mix Time 2 min. 2 min. 3 min. 4 min. 5 min. 5 min.
Wait 2 2 2 2 2 2 min. min. min. min. min. min.
探針校正
樣本分析
Day 2
結果輸出 最佳解決方案
實驗第一天 準備實驗分析所需要的細胞
XF生物能量測定儀專用培養盤 XF測定儀所使用的細胞培養盤與一般所使用的二 十四孔培養盤略有不同，原則上同樣具有4乘6的 格式，但是每個孔都具有類似漏斗的構造，底部 平坦的部分才是實際用於細胞培養的區域，其面 積與一般常用的九十六孔盤底面積相當。
預祝各位研究工作順利
David Li
尚博生物科技
海馬生物能量測定儀中文手冊
海馬生物能量測定儀實驗流程 貼附型細胞
Cell
Day 1
XF Analyzer
開機
訂定細胞數
分析培養基
背景校正設定 製備培養基 暖機
探針組
兩段式細胞培養 更換培養基 37 C無CO2 培養一小時
o
調整pH值
實驗流程設計
用于免疫学研究的安捷伦
* 摘自 Buck et al.(2017) Cell.Metabolic instruction of immunity, 169(4), 570-86
2500 寡霉素 FCCP + 抗霉素
2000
第 21 天
2500 2000
OCR (pmol/min)
谱系
300
寡霉素 FCCP * * * 鱼藤酮/抗霉素 A
200
*** * *
浆母细胞 活化的 B 细胞
100
0 0
***
** *
初始 B 细胞
20
40
60
80
100
时间 (min)
图 3. 安捷伦 Seahorse XF 细胞线粒体压力测试表明能量需求随着谱系定向而变化。 摘自 Price et al. (2018). Cell Rep. 23(11): 3152-9
免疫学研究人员使用安捷伦 Seahorse XF 细胞分析 技术来研究： – 免疫细胞生命周期、谱系和检查点 – 实时活化 – 代谢要求和燃料 – 代谢信号和通路 – 进行调控以控制结果
糖酵解与氧化磷酸化之比
效应 T 细胞
代谢活跃 糖酵解速率增加 备用呼吸能力降低 营养摄取增加 高度增殖
初始 T 细胞
4
代谢分析揭示了一组丰富的通路靶标，为免疫肿瘤学带 来新的治疗机会
了解干扰通路和控制免疫细胞反应的策略，带来治疗机会
PHD-4螺簇泄漏螺簇辅助仪器说明书
Advanced Test Equipment Rentals 800-404-ATEC (2832)®E s t a b l i s h e d 1981The Agilent AdvantageGlobal Application SupportExpertise When & Where You Need It• Thousands of portable SIPD sniffing heliumdetectors are in daily use worldwide • Helium leak testing is the preferred solution in a broad range of applications and industries • Native language application specialistsavailable locallyHigh Performance InstrumentsWide Range, PHD-4 Portable Helium Detector• High Sensitivity to Helium • Easy to Use • Truly Portable • Versatile • DependableIndustry Leading Service & SupportGet The Most From Your Investment• The system is designed to allow easy replacement ofsampling line components in the field• Exchange units are available for rapid field replacement•Support programs can be tailored to meet your most demanding needsMaximizing Productivity and UptimeFeatures and BenefitsVersatile - Suitable for many different applications• Wide range of uses: replaces or can be used with existing methods such as bubble test or pressure decay• Able to detect both very small and large leaks• Can operate either on battery power or connected to a mains power supply• Displayed messages can be viewed in several languages (English, French, German, Italian) •Standard Analog and RS232 Serial I/ODependable - Long term operation• Automatic backflow valve helps prevent helium saturation, ensuring fast recovery time aswell as long life of sensing element.•CE, CSA/US approved for global standardizationHigh Sensitivity to Helium - Can detect very small leaks• High Sensitivity (2 ppm) to helium, three orders of magnitude better than industry standard, due to SIPD (proprietary and patented Selective Ion Pump Detection)• Excellent selectivity for helium allows you to read helium leaks and ignore all other gases • Two levels of sensitivity are available for application dependent use•Autozero function allows leak detection even in unstable helium background environmentsEasy to Use - No training required• State-of-the-art microprocessor control allows great simplicity of operation • Fully automatic start-up with auto-diagnostics • Ready for test in less than 3 minutes • Intuitive display screen• Visual and audio indicators (standard headphone connection)• No tuning requiredTruly Portable - Compact and light• The PHD-4 weighs only 2,6 Kg (5.7 lbs) including the battery • Its compact size allows it to be easily carried anywhere• Its ergonomic design allows comfortable use for extended periodsThe New PHD-4 Portable Helium Detector 2133Large Vessels and BioreactorsThe PHD-4 offers unmatched accuracy and repeatability, presenting a unique solution that it is cost effective and very well suited for the leak range specifications of this application.Biotech and pharmaceutical industries used to rely on pressure decay and bubble test methods for finding leaks in their large bioreactors. The PHD-4 has established a new standard of quality, significantly increasing production yields.• Fermenters • Sterilizers •Freeze DryersUnderground Pipes and Storage TanksThe portability and light weight of the PHD-4 plays a major role in this application. Underground pipes and storage tanks (UST) are slightly pressurized with helium which, due to its high mobility, can escape through small leaks and migrate to the surface, where it can be easily detected by the PHD-4.The accuracy, portability and light weight of this unit greatly simplifies this process, particularly in difficult construction sites or rough terrain.• Gas distribution lines •Under and above ground containers and storage tanks •Telecommunication and high voltage underground cablesWater Heating and Cooling PipesThe PHD-4 allows leak location without interruption of the normal operation, by mixing helium with the water in the circuit. Until recently, the precise and rapid location of leaks in buried pipes has been very difficult.In the event of a leak, helium desorbs from the fluid and diffuses to the surface, where it is easily detected. Leaks in pipeline systems such as district heating systems, drinking or chilled water systems and steam pipe networks incur high costs due to losses and corrosion damage.• Heater exchangers and steam condensation lines •Water pipes •Radiant heating systemsAirplane Fuel Tanks and LinesPHD-4 technology is approved worldwide by airplane manufacturers and operators as the standard for the location of leaks in aircraft fuel tanks and in oxygen distribution lines. Agilent works with an exclusive distributor for aircraft applications. Please contact your local Agilent office for more information.•Fuel tanks •Oxygen distribution linesOther ApplicationsThe PHD-4 is in daily use in many other applications. Its portability makes it ideal for factory and field maintenance. Here is a partial list of other applications:• Components and systems for the Chemical and Petrochemical Industries • Compressed air components and delivery systems•Process gas delivery lines in Semiconductor fabrication industryCourtesy of Fraunhofer UMSICHT, GermanyApplicationThis information is subject to change without notice © Agilent Technologies, Inc. 2012Published February 29, 2012VPD-0112ENAgilent TechnologiesUSAAgilent Technologies 121 Hartwell Avenue,Lexington MA 02421, USA Tel: +1 781 861 7200Fax: +178****5437Toll free: +1 800 882 7426 ITALYAgilent Technologies Italia SpA via F.lli Varian 5410040 Leini, (Torino), Italy Tel: +39 011 9979 111Fax: +39 011 9979 350Toll free: 00 800 234 234 00BENELUXAgilent Technologies Netherlands B.V.Groenelaan 51186 AA Amstelveen Tel. +31 23 5377033Fax. +31 23 5382400Toll free: 00 800 234 234 00Agilent Technologies Belgium SA/NVPegasus Park, De Kleetlaan 5 bus 91831 Diegem - Belgium Tel. +31 23 5377033Fax +31 23 5382400Toll free: 00 800 234 234 00FRANCEAgilent Technologies France 7 avenue des TropiquesZ.A. de Courtaboeuf - B.P. 1291941 Les Ulis cedex, France Tel: +33 (0) 1 69 86 38 84Fax: +33 (0) 1 69 86 29 88Toll free: 00 800 234 234 00GERMANY and AUSTRIA Agilent TechnologiesSales & Services GmbH& Co. KG Lyoner Str. 2060 528 Frankfurt am Main, GERMANY Tel: +49 69 6773 43 2230Fax: +49 69 6773 43 2250Toll free: 00 800 234 234 00UK and IRELANDAgilent Technologies UK Ltd.6 Mead Road, Oxford Industrial Park Yarnton, Oxford OX5 1QU, UK Tel: +44 (0) 1865 291570Fax: +44 (0) 1865 291571Toll free: 00 800 234 234 00INDIAAgilent Technologies India Pvt. Ltd.G01. Prime corporate Park, 230/231,Sahar Rd., Opp. Blue Dart Centre,Andheri (East), Mumbai, 400 099 India Tel: +91 22 30648287/8200Fax: +91 22 30648250Toll free: 1800 113037CHINAAgilent Technologies (China) Co. LtdNo.3, Wang Jing Bei Lu,Chao Yang District,Beijing, 100102, China Tel: +86 (0)10 64397888Fax: +86 (0)10 64391318Toll free: 800 820 3278TAIWANAgilent Technologies Taiwan Limited20 Kao-Shuang Road Ping-Chen City, 32450Taiwan, R.O.C.Tel: +88 6 34959281Toll free: 0800 051 342JAPANAgilent Technologies Japan, Ltd.8th Floor Sumitomo Shibaura Building 4-16-36 Shibaura Minato-ku Tokyo 108-0023, Japan Tel: +81 3 5232 1253Fax: +81 3 5232 1710Toll free: 0120 655 040KOREAAgilent Technologies Korea Ltd.Shinsa 2nd Bldg. 1F 966-5 Daechi-dongKangnam-gu, Seoul, Korea 135-280Tel: +82 (0)2 2194 9449Fax: +82 (0)2 3452 3947Toll free: 080 222 2452SINGAPOREAgilent Technologies Singapore (Sales) Pte Ltd 1 Yishun Avenue 7Singapore 768923Tel : (65) 6377 1688DID: (65) 6215 8045Fax: (65) 6754 0574TollFree:180****2622SEAAgilent Technologies Sales (Malaysia) Sdn Bhd Unit 201 Level 2 Uptown 2,2 Jalan SS 21/37 Damansara Uptown 47400 Petaling Jaya, Selangor Malaysia.Tel: (60) 3 7727 8808Fax: (60) 3 7727 1209TollFree:180****2622The PHD-4 Portable Helium DetectorThe PHD-4 is a portable compact leak detector which includes a battery for autonomous use in the field and uses helium as a tracer gas. It allows detection of very small leaks in objects where a slight helium pressure has been introduced.Principle of operationThe PHD-4 principle of operation is based on a Varian patented technology, Selective Ion Pump Detection (SIPD).The sensor incorporates a quartz capillary tube maintained under high vacuum by an ion pump. The quartz tube is heated with a platinum filament and becomes permeable to helium. As the partial pressure of helium in the ion pump increases, so does the current drawn by the ion pump, proportional to the pressure, indicating the helium concentration present in the test probe of the PHD-4.WHY USE HELIUM AS A TRACER GAS?Helium is a superior choice as tracer gas for a number of reasons: • It is inert, non-toxic and non-flammable• It can pass easily through leaks due to its small atomic size, allowing the detection of very small leaks • It is present in the atmosphere at only 5 ppm, thus reducing the possibility of false readings • It is highly mobile, allowing rapid desorption and short measurement times•When used properly, it is the most economical and allows the highest sensitivity, of all trace gases。
flyer-xf-cell-mito-stress-test
Using the pre-calibrated, pre-tested reagents, the XF Cell Mito Stress Test Kit reveals the complete mitochondrial stress profile, while new algorithms automatically calculate the most important metrics, simplifying your work.Everything you need to measure mitochondrial function in cellsMeasurements of mitochondrial function are essential to understanding cancer, aging, and metabolic, cardiovascular, and neurodegenerative diseases. The XF Cell Mito Stress Test Kit makes it easy to measure the four key parameters of mitochondrial function in a microplate: basal respiration, ATP production, proton leak, and maximal respiration, revealing critical information not evident in basal metabolism measurements alone.Now you can confidently quantify cellular bioenergetics, identify mitochondrial dysfunction, and measure cells’ response to stress.Seahorse...the standard for measuring cell metabolism.“Without oxygen measurements, cellular bioenergetics would be in the dark ages.”—David Nicholls Buck Institute & Lund UniversityMito Stress Test ProfileBasal Respiration ATP ProductionProton Leak Maximal Respiration Spare Respiratory Capacity Non-mitochondrial RespirationTIME (minutes)O C R (p M o l e s /m i n )Mitochondrial Respiration& RotenoneNon-mitochondrial RespirationBasalRespiration ATP ProductionProton LeakMaximal RespirationSpareCapacityXF Cell Mito Stress Test Kit• Complete, easy-to-use kit for 6 microplates • Software wizard guides assay optimization,design, and interpretation• Designed for the XF e /XF AnalyzersXF Cell Mito Stress Test KitLearn more with our On-Demand Webinars at Profiling mitochondrial function has never been so easy: A tutorial on the new XF Cell Mito Stress Test KitDavid Ferrick, PhD, CSO, Seahorse BioscienceMitochondrial profiling in cells under physiological conditions and in response to stress Victor Darley-Usmar, PhD, University of Alabama at Birmingham Spare respiratory capacity and the bioenergetics of the nerve terminal David Nicholls PhD, FRSE, Buck Institute for Age Research & Lund University The rate and coupling efficiency of oxidative phosphorylation in mitochondria and cells Martin Brand, PhD, Buck Institute & MRC Dunn Nutrition UnitKit Contents• Pre-calibrated, pre-tested reagents:• Oligomycin (ATP Coupler)• FCCP (ETC Accelerator)• Antimycin A (Mito Inhibitor A)• Rotenone (Mito Inhibitor B)• DMSO (Solvent)Ordering InformationProduct: XF Cell Mito Stress Test Kit Part #: 101706-100Store at -20°CMeasure basal respiration, ATP production, proton leak, and spare respiratory capacity.Each XF Cell Mito Stress Test Kit eliminates the questions and concerns of where to source the reagents, what concentrations are optimal, and best storage conditions. Each convenient kit contains enough reagents to run sixmicroplates, allowing you to run a full mitochondrial profile in each well. With the reagents now ready at your fingertips, the XF Cell Mito Stress Test Kit is your ultimate tool for assay optimization.Primary Neonatal Rat Ventricular MyocytesRat Aortic Smooth Muscle CellsSpare Capacity Coupling Efficiency Proton LeakNon-Mitochondrial RespirationMito Stress Test Measures201202063Seahorse Bioscience Europe Symbion Science Park, Boks 22Fruebjergvej 32100 Copenhagen DK +45 31 36 98 78Seahorse Bioscience Asia 199 Guo Shou Jing Road Suite 207Pudong, Shanghai 201203 CN 0086 21 33901768Seahorse Bioscience Inc.16 Esquire RoadNorth Billerica, MA 01862 US +1-978-671-1600800-671-0633。
Seahorse使用前准备物品清单
glucose, L-glutamine, sodium pyruvate用于配制检测液，同时准备1N NaOH用于调pH值。
检测液配制方法见培训手册”Preparation of Assay Media for Use in XFp Assays”。
【注意】sodium pyruvate可以准备粉末，但我们更推荐购买sodium pyruvate 100mM 液体包装，使用方便。
注意购买后分装保存。
上述试剂配制都不需要无菌操作。
3.仪器和耗材：
无二氧化碳37度培养箱，pH计，细胞计数仪（或计数板），37度水浴锅，显微镜，
涡旋混合器，移液枪，0.22uM滤器，10ml灭菌管6个，1.5ml EP管若干。
【注意】请确认PH计是准确的好用的，提前使用校准液校准。
Seahorse实验要求精确测量PH值。
4.细胞：
我们曾经做过的效果比较好的细胞有C2C12，HepG2等。
请告知您实验室培养的细胞系有哪些，我们将采用贴壁牢的细胞进行培训及相关实验。
LCMS液质联用仪原理及基础知识介绍
L C /M S 原理及基础知识介绍沃特斯中国有限公司应用部W a t e r s C h i n a L t d .M S 的历史1910年世界上第一台现代意义质谱仪在英国剑桥 C a v e n d i s h 实验室出现 1917年电喷雾 (E l e c t r o s p r a y 物理现象被发现 (并非为了 M S1918年世界上第一台实际意义质谱仪在美国芝加哥大学实验室出现 (扇型磁场 M S1943年世界上第一台商业质谱仪 1953年四极杆质量分析器质谱仪 1955年飞行时间质量分析器质谱仪1960’ s 开发 G C /MS 60’ s -70’ s 大气压电离 (A P c I 源被发现 (但并未被广泛应用70’ s -80’ s 开始广泛研究 L C /MS 1979年传送带式 L C /M S 接口成为商业产品 1982年离子束 L C /M S 接口出现 1984年第一台电喷雾 M S 仪宣告诞生 1988年电喷雾离子源 M S 首次应用于蛋白质的分析… . . .W a t e r s C h i n a L t d . L C /M S 中的液相色谱H i g h P e r f o r m a n c e L i q u i dC h r o m a t o g r a p h y -高效液相色谱液质联用的前提和基础 =进样 +分离根据化合物的化学特性分离样品 (比如极性化合物 , 非极性化合物 , 酸性化合物 , 碱性化合物等等• 分离技术• 分离效率高• 流动相参与分离• 连续流出 , 峰宽有限• 有时需要使用缓冲盐提高分离度• 高压环境工作 (> 1000 p s iW a t e r s C h i n a L t d .L C /M S 中的质谱M a s s S p e c t r o m e t r y -质谱质量是物质固有特征之一 , 不同的物质有不同的质量谱分析物被转化为气相离子而被分析这些离子按其质荷比 (m /z 被分离而被检测质谱图即是相关的离子流对 m /z 的图• 灵敏度高• 定性 /定量本领高• 脉冲扫描采集数据• 高真空环境工作• 溶剂参与反应W a t e r s C h i n a L t d .L C /M S 数据形式G a m a b u f o t a l i n 保留时间W a t e r s C h i n a L t d .质 谱 的 种 类 及 相 关 联 用 技 术液质联用系统的常见部件离子化质子过滤器离子检测器色谱分离样品引入数据采集及控制W a t e r s C h i n a L t d . 从 L C 到 M S 的转变物态转变 : 液态 -气态带电状态: “ 中性” -离子真空变化 : 760 t o r r -10-5t o 10-8t o r rW a t e r s C h i n a L t d .L C /M S 系统构成E I 电离源样品引入A P I 电离源样品引入W a t e r s C h i n a L t d .传统的样品电离方式 :E I 源E I 源 :电子轰击电离源可得经典的质谱谱图可查询质谱库 , 质谱结果可用工业标准谱库检索 , 是绝对的化合物鉴定相对易得 , 耐受性好谱图可解析 , 阐明结构信息但是 , 电离效率较差 , 测定的分子量范围、流速范围有限 ; 可测的化合物类型有限样品灯丝集电极离子聚焦透镜子去析器-E I 源 L C /M S 系统 :W a t e r s I n t e g r i t y 系统E I 的 宝 贵 遗 产HO O G a m a b u f o t a l i n (I I IM -18+M +M -36+341402 38436691 107123145 175191 79201135M - 162 +24191 107 123145 175191 79201 34138436M -18 +M +M -36+M - 162+24102H i g h R e s M a s s S p e c , B r o w n , K a m a n o , P e t t i t , O r g . M a s s S p e c . , 1972W a t e r s I n t e g r i t y L C /M S S y s t e m , P f i z e r , 1996W a t e r s C h i n a L t d .大气压电离技术电喷雾电离 (E l e c t r o s p r a y I o n i z a t i o n - 大气压化学电离(A t m o s p h e r i c P r e s s u r e C h e m i c a l I o n i z a t i o n -相转化过程W a t e r s C h i n a L t d .3 离子从液滴表面蒸发出来1 含各种离子的液滴-++++-----+++--++++-----+++-2 随着液滴的挥发 , 电荷密度增大 , 离子集中到液滴表面++-+-+++-++-+-+++-W a t e r s C h i n a L t d .x xx H H +M1 电离产生起决定作用的溶剂离子2 样品分子与反应离子发生反应 , 形成离子簇M H +xx xxX H +xx H +M H +3 离子去簇后被传输到质谱的质量分析器X = 溶剂分子 , 比如 H 2O , N H 3等等一般适合分析挥发性化合物 , 也常分析从中性到极性的化合物纯气相离子化过程 , 只产生极少的添加离子谱图简单流速范围大 , 从 0.2 到 2. 0m L /m i n , 而不用分流W a t e r s C h i n a L t d . A P I 电 离 源 (E S I + 电 离 模 式 :软 电 离 产 生 [M +H ]+准 分 子 离 子 峰W a t e r s C h i n a L t d .A l l i a n c e Z Q L C /M S 系统W a t e r s C h i n a L t d . W a t e r s Z Q . . .W a t e r s C h i n a L t d .同 一 样 品 的 质 谱 图 可 能 并 不 一 样 !S a m p l e D e s c r i p t i o n80150 m /z 10S A L I C Y L A M I D E 1 (0. 067 C n (C e n , 2, 80. 00, A rS c a n E S + 1. 19e 8138. 01370121. 2100. 290. 18818293. 9. 8114. 0110. 22. 013303. 98. 9143. 0149. 32-H y d r o x y b e n z a m i d e M W : 137 F o r m : C 7H 7 N O 2 C A S R e g N o : 65-45-225225250162839658092120137C O N H 2O HF i g u r e 1. W i l e y L i b r a r y R e f e r e n c e S p e c t r u m o f S a l i c y l a m i d eW a t e r s C h i n a L t d .C ID 产生的过程A n a l y s i s o f S u l f a m e t h a z i n e b y E l e c t r o s p r a y1000, 000, 000, 000S u l f a m e t h a z i n e[M +H ]+2772782792802812822, 0004, 0006, 0008, 00010, 000000P a r e n t I o n R e g i o nM W 278m /zP o s i t i v e i o n m o d e , E S I , 2 u l /m i n , 50% M e O H :49% H 2O :1% H O A c, 00012, 0, 000, 000150200250300m /zW a t e r s C h i n a L t d .优异的 C I D 技术 (可编程A n a l y s i s o f S u l f a m e t h a z i n e b y E l e c t r o s p r a yS u l f a m e t h a z i n e 10002, 0004, 0006, 0008, 00010, 000[M +H ]+2, 0004, 0006, 0008, 00010, 000F r a g m e n I o n R e g i o nM W 186m /zC 6H 8N 3O 2S150200250300m /zW a t e r s C h i n a L t d .多种 C I D 方式 :一次进样得到全部质谱信息C 4H 9-W a t e r s C h i n a L t d .四极杆质量分析器W a t e r s C h i n a L t d .L C /M S 联用中的转变过程液相色谱的现实情况 :从高效液相色谱流出的化合物存在于大气压条件下的溶液之中质谱的现实情况 :在高真空工作条件下的质谱仅接受气相的离子因此液质联用的接口必须完成:去除溶剂保留样品电离化合物W a t e r s C h i n a L t d .L C /M S 面临的挑战W a t e r s C h i n a L t d .接口E I 电离源的液质联用接口 -热束接口 (T B 雾化 , 去溶剂然后电离E S I 和 A P C I 接口 -Z S p r a y 雾化 , 去溶剂和电离同时完成W a t e r s C h i n a L t d .热束接口原理 :喷雾作用和动量分离雾化室动量分离器 (第 1 & 2级压力为 5 T o r 并且 90% 的溶剂和载气(H e 被去除, 压力为 1. 5 T o r r 并且剩余的 1 0%溶剂和氦气被去除。
seahorse xf pro工作原理
Seahorse XF Pro是一款高端的代谷细胞分析仪，它采用了新颖的技术，可以实时监测细胞的代谢状态和功能。
Seahorse XF Pro的工作原理是基于代谷细胞的特异性代谷测定技术，它能够实时测定细胞内ATP产生速率和氧化磷酸化水平，从而揭示细胞的能量代谢和功能活性。
下面将逐步介绍Seahorse XF Pro的工作原理：1. 原理概述Seahorse XF Pro利用代谷细胞分析技术，通过测定细胞内ATP产生速率和氧化磷酸化水平来实时监测细胞的能量代谢和功能活性。
它采用了荧光探针技术和高通量检测系统，可以在短时间内对细胞进行多参数测定，为细胞代谷研究提供了重要的工具和技术支持。
2. 测定原理Seahorse XF Pro通过测定细胞内ATP产生速率和氧化磷酸化水平来实时监测细胞的代谢状态和功能活性。
它利用荧光探针技术，通过不同荧光信号的变化来反映细胞内代谷物质的浓度和动态变化。
Seahorse XF Pro采用了高通量检测系统，可以对多个样品进行同时测定，提高了测定效率和数据可靠性。
3. 适用范围Seahorse XF Pro主要应用于生物医学研究领域，可以用于细胞代谷、药物筛选、毒性评价和疾病模型研究等方面。
它可以对不同类型的细胞和生物样品进行分析，为科研人员和生物制药企业提供了一种快速、准确、可靠的细胞功能分析方法。
4. 工作流程Seahorse XF Pro的工作流程一般包括样品处理、实验设置、反应测定和数据分析四个步骤。
在样品处理阶段，需要对细胞进行适当的处理和预处理，以保证实验的准确性和可靠性。
在实验设置阶段，需要根据实验的要求进行合理的参数设置和实验方案设计。
在反应测定阶段，需要将样品加入到测定盘中，通过Seahorse XF Pro进行代谷测定实验。
在数据分析阶段，需要对测定结果进行分析和解读，得出实验结论和科研成果。
5. 应用前景Seahorse XF Pro作为一种新型的细胞分析仪器，具有广阔的应用前景和市场潜力。
安捷伦 Seahorse XF 底物氧化压力测试试剂盒说明书
一种通过底物氧化来评估线粒体代谢的创新方法安捷伦 Seahorse 提供了一套强大的新分析方法，通过评估活细胞中耗氧率 (OCR) 的变化来测定细胞底物氧化。
这些快速的非破坏性方法可以研究细胞如何氧化 3 种主要的线粒体底物：长链脂肪酸 (LCFA)、葡萄糖/丙酮酸和/或谷氨酰胺，从而揭示通路的特异性信息。
这些分析方法提供了直观的功能参数，这些参数建立在广受认可的 Seahorse XF 细胞线粒体压力测试基础上，无论是在基础条件下还是在响应升高的底物需求时，都非常适用于评估细胞的底物需求。
利用标准化的分析方案、经验证的试剂和基于云的 Seahorse Analytics ，您可以开始进行测试，并快速获得条理清晰的出色结果。
深入揭示影响细胞命运、功能和适应性的因素LCFA 、葡萄糖/丙酮酸和谷氨酰胺是线粒体代谢的 3 种主要底物。
许多领域（如癌症、免疫学、干细胞生物学）的研究人员已经证明了这些底物的氧化如何对细胞命运、功能和适应性产生深远影响。
许多发现不仅提供了新的生物学见解，而且揭示了用于干预和开发成功疗法的新方法。
Seahorse 底物氧化压力测试试剂盒能够快速评估活细胞中的底物氧化，有助于研究细胞如何改变或转变特定底物的氧化来执行激活、增殖和分化等重要的细胞功能，细胞在微环境中的生存，以及对遗传或药物干预的响应。
了解更多信息：/chem/discoverXF安捷伦 Seahorse XF 底物氧化压力测试– 揭示细胞对线粒体底物（LCFA 、葡萄糖/丙酮酸和/或谷氨酰胺）的依赖性– 了解哪些底物与特定的细胞功能和/或表型相关– 借助用于测定后分析的集成数据处理工具轻松实现测定– 经优化的一次性试剂可简化工作流程并降低分析复杂性了解更多信息/chem/discoverXF 全球技术支持********************************安捷伦客户服务中心免费专线：800-820-3278400-820-3278（手机用户）仅限研究使用。
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Seahorse Bioscience XFp
ico orce 米力光国际贸易有限公司
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Analyzer
XFe96
XFe24
Seahorsebio
Seahorse
Bioscience
XFp Analyzer 细胞外流量分析仪
The XFp Analyzer features fast and easy setup using built-in assay templates and a miniplate format, making it ideal for researchers who run just a few samples per day. Key applicati include metabolic phenotyping of rare cell types, patient-derived samples, and other precious cells. The XFp Analyzer is our most affordable XF platform and is suitable for performing our gold standard Stress Tests in a pairwise comparison format.
M
ico orce 米力光国际贸易有限公司
f
Key Applications for the XFp Analyzer
Seahorse XF technology delivers functional metabolic parameters that have become the gold standard for energy metabolism. With the XFp Analyzer it is easier than ever to complete your research story with these standard assays:
XFp FluxPaks and XFp Miniplates provide a compact format that can be set up quickly and easily. With six assay wells, the XFp Miniplate has the same well geometry as the XFe96 microplate, making the XFp convenient for pilot experiments and iterative assay design. The XFp Analyzer easily performs the Gold standard XF assays (metaboless Test and XF Glycolysis Stress Test) on small sample sets, making it ideal for: Pairwise comparisons Phenotyping Patient-derived samples
XFp Extracellular Flux Analyzer
Proven XF Technology, Accessible for Every Lab
Contact Me About XFp
XFp Analyzer
M
ico orce 米力光国际贸易有限公司
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XFp Miniplate and sensor cartridge For researchers analyzing precious, limited biomaterial or who just prefer to run a few samples per day, the XFp Analyzer provides powerful XF technology in a streamlined, easy-to-use platform. The XFp Extracellular Flux Analyzer is built on proven XF technology for monitoring the two major energy pathways of the cell: Oxidative Phosphorylation, measured by Oxygen Consumption Rate (OCR) and Glycolysis, measured by Extracellular Acidification Rat e (ECAR). Benefits of the new XFp Analyzer: Allows quick assessment of a few samples Runs standard assays without need for special bioenergetics expertise Affordable by individual labs
XF Glycolysis Stress Test Analyze Metabolic Reprogramming in Primary Immune Cells In order to respond to foreign antigens, immune cells must be able to increase their metabolism. In these mouse bone-marrow derived macrophages, overnight activation by LPS revved up the glycolytic machinery. The XFp detected an increase in both glycolysis (ECAR) and glycolytic reserve compared to quiescent cells.
XF Phenogram Detect metabolic switching in a single pairwise experiment
M
ico orce 米力光国际贸易有限公司
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Upon induction of certain signaling pathways, cells will shift their mode of energy production. Activation of HIF- 1α by CoCl2 induces a metabolic switch in SKOV-3 cancer cells. The XF Phenogram shows that OXPHOS (OCR) of the treated cells is reduced and glycolysis (ECAR) is increased relative to the control.
XF Cell Mito Stress Test Verify impact of gene expression changes on metabolicphenotype Oxidative phosphorylation (OXPHOS) requires the mitochondrial electron transport chain as well as access to substrates such as pyruvate. Inhibition of mitochondrial pyruvate carrier 1 (MPC1) by UK5099 has no impact on basal OXPHOS but spare respiratory capacity is abrogated in C2C12-derived myotubes.