DIP_Pratt_P6
载体及其上下游引物
BGH rev/c-mycrev
pcDNA3.1/V5-His(_A,_B,_C)
T7/CMV-Profor
BGH rev/V5rev
pcDNA3.1/Hygro(+,-)
T7/CMV-Profor
BGH rev/V5rev
pcDNA3.1/Zeo(+.-)
T7
BGHrev
pCAMBIA1301(1300)
pCAMBIA1301-5'(ECORI)/M13R
pCAMBIA1301-3'(HindIII)
pCAMBIA1304
pCAMBIA1301-5'(ECORI)
pCAMBIA1301-3'(HindIII)
pCAMBIA2300
M13R(-48)
M13F(-47)/M13F(-49)
pACT2
GAL4 ADfor
pGAL4-ADrv
pACT2
p17110
p12584
pACYC184(BamHI-site)
PBRforBam
PBRrevBam
pACYCDuet-1(MCSI)
ACYCDuetUP1 Primer
DuetDOWN1
pACYCDuet-1(MCSII)
DuetUp2
T7-Terminator
pDEST26
TREfor/CMV-Profor
T7/M13for
pDisplay
T7/CMV-Profor
c-mycrev
pDNR-LIB(MCS_A)
M13for,T7
pSG5-3,M13R
pDonar
M13F
SN74AHCT1G125 单路总线缓冲器 线驱动器说明书
SN74AHCT1G125SINGLE BUS BUFFER GATE WITH 3ĆSTATE OUTPUTSCLS378L − AUGUST 1997 − REVISED JUNE 2005D Operating Range of 4.5 V to 5.5 V D Max t pd of 6 ns at 5 V D Low Power Consumption, 10-µA Max I CCD ±8-mA Output Drive at 5 VD Inputs Are TTL-Voltage Compatible D Latch-Up Performance Exceeds 250 mA Per JESD 1732451OE V CC Y A GND DBV PACKAGE(TOP VIEW)DCK PACKAGE(TOP VIEW)32451OEV CC YAGND 32451OE V CC Y A GND DRL PACKAGE (TOP VIEW)See mechanical drawings for dimensions.description/ordering informationThe SN74AHCT1G125 is a single bus buffer gate/line driver with 3-state output. The output is disabled whenthe output-enable (OE) input is high. When OE is low, true data is passed from the A input to the Y output.To ensure the high-impedance state during power up or power down, OE should be tied to V CC through a pullupresistor; the minimum value of the resistor is determined by the current-sinking capability of the driver.ORDERING INFORMATIONT A PACKAGE †ORDERABLE PART NUMBER TOP-SIDE MARKING ‡Reel of 3000SN74AHCT1G125DBVR SOT (SOT-23) − DBVReel of 250SN74AHCT1G125DBVT B25_°°Reel of 3000SN74AHCT1G125DCKR −40C to 85C SOT (SC-70) − DCKReel of 250SN74AHCT1G125DCKT BM_SOT (SOT-553) − DRL Reel of 4000SN74AHCT1G125DRLR BM_†Package drawings, standard packing quantities, thermal data, symbolization, and PCB design guidelines areavailable at /sc/package.‡The actual top-side marking has one additional character that designates the assembly/test site.FUNCTION TABLEINPUTSOUTPUT OEA Y LH H LL L H X Zlogic diagram (positive logic)A YOE124Copyright 2005, Texas Instruments IncorporatedPRODUCTION DATA information is current as of publication date.Please be aware that an important notice concerning availability, standard warranty, and use in critical applications ofTexas Instruments semiconductor products and disclaimers thereto appears at the end of this data sheet.SN74AHCT1G125SINGLE BUS BUFFER GATEWITH 3ĆSTATE OUTPUTSCLS378L − AUGUST 1997 − REVISED JUNE 2005absolute maximum ratings over operating free-air temperature range (unless otherwise noted)†Supply voltage range, V CC−0.5 V to 7 V. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Input voltage range, V I (see Note 1) −0.5 V to 7 V. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Output voltage range, V O (see Note 1) −0.5 V to V CC+ 0.5 V. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Input clamp current, I IK(V I< 0) −20 mA. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Output clamp current, I OK(V O< 0 or V O > V CC) ±20 mA. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Continuous output current, I O(V O= 0 to V CC) ±25 mA. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Continuous current through V CC or GND ±50 mA. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Package thermal impedance, θJA (see Note 2):DBV package 206°C/W. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .DCK package 252°C/W. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .DRL package 142°C/W. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Storage temperature range, T stg −65°C to 150°C. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .†Stresses beyond those listed under “absolute maximum ratings” may cause permanent damage to the device. These are stress ratings only, and functional operation of the device at these or any other conditions beyond those indicated under “recommended operating conditions” is not implied. Exposure to absolute-maximum-rated conditions for extended periods may affect device reliability.NOTES: 1.The input and output voltage ratings may be exceeded if the input and output current ratings are observed.2.The package thermal impedance is calculated in accordance with JESD 51-7.recommended operating conditions (see Note 3)MIN MAX UNITV CC Supply voltage 4.5 5.5VV IH High-level input voltage2VV IL Low-level input voltage0.8VV I Input voltage0 5.5VV O Output voltage0V CC VI OH High-level output current−8mAI OL Low-level output current8mA∆t/∆v Input transition rise or fall rate20ns/V T A Operating free-air temperature−4085°C NOTE 3:All unused inputs of the device must be held at V CC or GND to ensure proper device operation. Refer to the TI application report, Implications of Slow or Floating CMOS Inputs, literature number SCBA004.electrical characteristics over recommended operating free-air temperature range (unless otherwise noted)CC T A = 25°CPARAMETER TEST CONDITIONS VMIN TYP MAXMIN MAX UNITV I OH = −50 m A 4.4 4.5 4.4OH IOH = −8 mA 4.5 V3.94 3.8VV I OL = 50 m A0.10.1OL IOL = 8 mA 4.5 V0.360.44VI I V I = 5.5 V or GND0 V to 5.5 V±0.1±1m AI OZ V O = V CC or GND 5.5 V±0.25±2.5m AI CC V I = V CC or GND,I O = 0 5.5 V110m A∆I CC‡One input at 3.4 V,Other input at V CC or GND 5.5 V 1.35 1.5mAC i V I = V CC or GND 5 V41010pFC o V O = V CC or GND 5 V10pF ‡This is the increase in supply current for each input at one of the specified TTL voltage levels, rather than 0 V or V CC.SN74AHCT1G125SINGLE BUS BUFFER GATE WITH 3ĆSTATE OUTPUTSCLS378L − AUGUST 1997 − REVISED JUNE 2005switching characteristics over recommended operating free-air temperature range,V CC = 5 V ±0.5 V (unless otherwise noted) (see Figure 1)FROM TO LOAD T A = 25°C PARAMETER(INPUT)(OUTPUT)CAPACITANCE MIN TYP MAX MIN MAX UNIT t PLH3.8 5.51 6.5t PHLA Y C L = 15 pF 3.8 5.51 6.5ns t PZH3.6 5.116t PZLOE Y C L = 15 pF 3.6 5.116ns t PHZL 4.6 6.818t PLZOE Y C = 15 pF 4.6 6.818ns t PLH5.37.518.5t PHLA Y C L = 50 pF 5.37.518.5ns t PZH5.17.118t PZLOE Y C L = 50 pF 5.17.118ns t PHZL 6.18.8110t PLZ OE Y C = 50 pF 6.18.8110nsoperating characteristics, V CC = 5 V, T A = 25°CPARAMETERTEST CONDITIONS TYP UNIT C pd Power dissipation capacitance No load, f = 1 MHz 14pFSN74AHCT1G125SINGLE BUS BUFFER GATE WITH 3ĆSTATE OUTPUTSCLS378L − AUGUST 1997 − REVISED JUNE 2005PARAMETER MEASUREMENT INFORMATION50% V CC 3 V3 V 0 V0 Vt h t suVOLTAGE WAVEFORMSSETUP AND HOLD TIMESData Inputt PLH t PHL t PHL t PLH V OH V OH V OL V OL 3 V 0 V 50% V CC50% V CC Input Out-of-Phase Output In-Phase Output Timing Input 50% V CC VOLTAGE WAVEFORMSPROPAGATION DELAY TIMESINVERTING AND NONINVERTING OUTPUTS OutputControlOutput Waveform 1S1 at V CC (see Note B)OutputWaveform 2S1 at GND(see Note B)V OL V OH t PZLt PZHt PLZ t PHZ ≈V CC 0 V 50% V CCV OL + 0.3 V 50% V CC ≈0 V 3 V VOLTAGE WAVEFORMS ENABLE AND DISABLE TIMES LOW- AND HIGH-LEVEL ENABLINGt PLH /t PHL t PLZ /t PZLt PHZ /t PZHOpen Drain Open V CC GND V CCTEST S13 V 0 V t w VOLTAGE WAVEFORMS PULSE DURATION Input NOTES: A.C L includes probe and jig capacitance.B.Waveform 1 is for an output with internal conditions such that the output is low, except when disabled by the output control.Waveform 2 is for an output with internal conditions such that the output is high, except when disabled by the output control.C.All input pulses are supplied by generators having the following characteristics:PRR ≤ 1 MHz, Z O = 50 Ω, t r ≤3 ns, t f ≤ 3 ns.D.The outputs are measured one at a time, with one input transition per measurement.E.All parameters and waveforms are not applicable to all devices.From Output Under Test C L (see Note A)LOAD CIRCUIT FOR 3-STATE AND OPEN-DRAIN OUTPUTSS1V CC R L = 1 k ΩGND From OutputUnder TestC L (see Note A)Test Point LOAD CIRCUIT FORTOTEM-POLE OUTPUTS OpenV OH − 0.3 V 1.5 V 1.5 V 1.5 V1.5 V 1.5 V 1.5 V 1.5 V 1.5 V 1.5 V Figure 1. Load Circuit and Voltage WaveformsPACKAGING INFORMATION(1) The marketing status values are defined as follows:ACTIVE: Product device recommended for new designs.LIFEBUY: TI has announced that the device will be discontinued, and a lifetime-buy period is in effect.NRND: Not recommended for new designs. Device is in production to support existing customers, but TI does not recommend using this part in a new design. PREVIEW: Device has been announced but is not in production. Samples may or may not be available.OBSOLETE: TI has discontinued the production of the device.Addendum-Page 1(2) Eco Plan - The planned eco-friendly classification: Pb-Free (RoHS), Pb-Free (RoHS Exempt), or Green (RoHS & no Sb/Br) - please check /productcontent for the latest availability information and additional product content details.TBD: The Pb-Free/Green conversion plan has not been defined.Pb-Free (RoHS): TI's terms "Lead-Free" or "Pb-Free" mean semiconductor products that are compatible with the current RoHS requirements for all 6 substances, including the requirement that lead not exceed 0.1% by weight in homogeneous materials. Where designed to be soldered at high temperatures, TI Pb-Free products are suitable for use in specified lead-free processes.Pb-Free (RoHS Exempt): This component has a RoHS exemption for either 1) lead-based flip-chip solder bumps used between the die and package, or 2) lead-based die adhesive used between the die and leadframe. The component is otherwise considered Pb-Free (RoHS compatible) as defined above.Green (RoHS & no Sb/Br): TI defines "Green" to mean Pb-Free (RoHS compatible), and free of Bromine (Br) and Antimony (Sb) based flame retardants (Br or Sb do not exceed 0.1% by weight in homogeneous material)(3) MSL, Peak Temp. - The Moisture Sensitivity Level rating according to the JEDEC industry standard classifications, and peak solder temperature.(4) There may be additional marking, which relates to the logo, the lot trace code information, or the environmental category on the device.(5) Multiple Device Markings will be inside parentheses. Only one Device Marking contained in parentheses and separated by a "~" will appear on a device. If a line is indented then it is a continuation of the previous line and the two combined represent the entire Device Marking for that device.(6) Lead/Ball Finish - Orderable Devices may have multiple material finish options. Finish options are separated by a vertical ruled line. Lead/Ball Finish values may wrap to two lines if the finish value exceeds the maximum column width.Important Information and Disclaimer:The information provided on this page represents TI's knowledge and belief as of the date that it is provided. TI bases its knowledge and belief on information provided by third parties, and makes no representation or warranty as to the accuracy of such information. Efforts are underway to better integrate information from third parties. TI has taken and continues to take reasonable steps to provide representative and accurate information but may not have conducted destructive testing or chemical analysis on incoming materials and chemicals. TI and TI suppliers consider certain information to be proprietary, and thus CAS numbers and other limited information may not be available for release.In no event shall TI's liability arising out of such information exceed the total purchase price of the TI part(s) at issue in this document sold by TI to Customer on an annual basis.OTHER QUALIFIED VERSIONS OF SN74AHCT1G125 :•Automotive: SN74AHCT1G125-Q1NOTE: Qualified Version Definitions:•Automotive - Q100 devices qualified for high-reliability automotive applications targeting zero defectsAddendum-Page 2TAPE AND REEL INFORMATION*All dimensions are nominal Device Package Type Package DrawingPinsSPQ Reel Diameter (mm)Reel Width W1(mm)A0(mm)B0(mm)K0(mm)P1(mm)W (mm)Pin1Quadrant SN74AHCT1G125DBVR SOT-23DBV 53000180.09.2 3.17 3.23 1.37 4.08.0Q3SN74AHCT1G125DBVR SOT-23DBV 53000178.09.2 3.3 3.2 1.55 4.08.0Q3SN74AHCT1G125DBVR SOT-23DBV 53000180.08.4 3.23 3.17 1.37 4.08.0Q3SN74AHCT1G125DBVT SOT-23DBV 5250178.09.0 3.23 3.17 1.37 4.08.0Q3SN74AHCT1G125DBVT SOT-23DBV 5250178.09.2 3.3 3.2 1.55 4.08.0Q3SN74AHCT1G125DCKR SC70DCK 53000180.09.2 2.3 2.55 1.2 4.08.0Q3SN74AHCT1G125DCKR SC70DCK 53000178.09.2 2.4 2.4 1.22 4.08.0Q3SN74AHCT1G125DCKT SC70DCK 5250178.09.0 2.4 2.5 1.2 4.08.0Q3SN74AHCT1G125DCKT SC70DCK 5250180.09.2 2.3 2.55 1.2 4.08.0Q3SN74AHCT1G125DCKT SC70DCK 5250178.09.2 2.4 2.4 1.22 4.08.0Q3SN74AHCT1G125DRLR SOTDRL 54000180.08.4 1.98 1.780.69 4.08.0Q3SN74AHCT1G125DRLR SOT DRL 54000180.09.5 1.78 1.780.69 4.08.0Q3*All dimensions are nominalDevice Package Type Package Drawing Pins SPQ Length(mm)Width(mm)Height(mm) SN74AHCT1G125DBVR SOT-23DBV53000205.0200.033.0SN74AHCT1G125DBVR SOT-23DBV53000180.0180.018.0SN74AHCT1G125DBVR SOT-23DBV53000202.0201.028.0SN74AHCT1G125DBVT SOT-23DBV5250180.0180.018.0SN74AHCT1G125DBVT SOT-23DBV5250180.0180.018.0SN74AHCT1G125DCKR SC70DCK53000205.0200.033.0SN74AHCT1G125DCKR SC70DCK53000180.0180.018.0SN74AHCT1G125DCKT SC70DCK5250180.0180.018.0SN74AHCT1G125DCKT SC70DCK5250205.0200.033.0SN74AHCT1G125DCKT SC70DCK5250180.0180.018.0SN74AHCT1G125DRLR SOT DRL54000202.0201.028.0SN74AHCT1G125DRLR SOT DRL54000184.0184.019.0IMPORTANT NOTICETexas Instruments Incorporated and its subsidiaries(TI)reserve the right to make corrections,enhancements,improvements and other changes to its semiconductor products and services per JESD46,latest issue,and to discontinue any product or service per JESD48,latest issue.Buyers should obtain the latest relevant information before placing orders and should verify that such information is current and complete.All semiconductor products(also referred to herein as“components”)are sold subject to TI’s terms and conditions of sale supplied at the time of order acknowledgment.TI warrants performance of its components to the specifications applicable at the time of sale,in accordance with the warranty in TI’s terms and conditions of sale of semiconductor products.Testing and other quality control techniques are used to the extent TI deems necessary to support this warranty.Except where mandated by applicable law,testing of all parameters of each component is not necessarily performed.TI assumes no liability for applications assistance or the design of Buyers’products.Buyers are responsible for their products and applications using TI components.To minimize the risks associated with Buyers’products and applications,Buyers should provide adequate design and operating safeguards.TI does not warrant or represent that any license,either express or implied,is granted under any patent right,copyright,mask work right,or other intellectual property right relating to any combination,machine,or process in which TI components or services are rmation published by TI regarding third-party products or services does not constitute a license to use such products or services or a warranty or endorsement e of such information may require a license from a third party under the patents or other intellectual property of the third party,or a license from TI under the patents or other intellectual property of TI.Reproduction of significant portions of TI information in TI data books or data sheets is permissible only if reproduction is without alteration and is accompanied by all associated warranties,conditions,limitations,and notices.TI is not responsible or liable for such altered rmation of third parties may be subject to additional restrictions.Resale of TI components or services with statements different from or beyond the parameters stated by TI for that component or service voids all express and any implied warranties for the associated TI component or service and is an unfair and deceptive business practice. TI is not responsible or liable for any such statements.Buyer acknowledges and agrees that it is solely responsible for compliance with all legal,regulatory and safety-related requirements concerning its products,and any use of TI components in its applications,notwithstanding any applications-related information or support that may be provided by TI.Buyer represents and agrees that it has all the necessary expertise to create and implement safeguards which anticipate dangerous consequences of failures,monitor failures and their consequences,lessen the likelihood of failures that might cause harm and take appropriate remedial actions.Buyer will fully indemnify TI and its representatives against any damages arising out of the use of any TI components in safety-critical applications.In some cases,TI components may be promoted specifically to facilitate safety-related applications.With such components,TI’s goal is to help enable customers to design and create their own end-product solutions that meet applicable functional safety standards and requirements.Nonetheless,such components are subject to these terms.No TI components are authorized for use in FDA Class III(or similar life-critical medical equipment)unless authorized officers of the parties have executed a special agreement specifically governing such use.Only those TI components which TI has specifically designated as military grade or“enhanced plastic”are designed and intended for use in military/aerospace applications or environments.Buyer acknowledges and agrees that any military or aerospace use of TI components which have not been so designated is solely at the Buyer's risk,and that Buyer is solely responsible for compliance with all legal and regulatory requirements in connection with such use.TI has specifically designated certain components as meeting ISO/TS16949requirements,mainly for automotive use.In any case of use of non-designated products,TI will not be responsible for any failure to meet ISO/TS16949.Products ApplicationsAudio /audio Automotive and Transportation /automotiveAmplifiers Communications and Telecom /communicationsData Converters Computers and Peripherals /computersDLP®Products Consumer Electronics /consumer-appsDSP Energy and Lighting /energyClocks and Timers /clocks Industrial /industrialInterface Medical /medicalLogic Security /securityPower Mgmt Space,Avionics and Defense /space-avionics-defense Microcontrollers Video and Imaging /videoRFID OMAP Applications Processors /omap TI E2E Community Wireless Connectivity /wirelessconnectivityMailing Address:Texas Instruments,Post Office Box655303,Dallas,Texas75265Copyright©2014,Texas Instruments Incorporated。
SpicaTM Gen2 PAM4 DSP for 800G Optical Module Appl
Product Brief Spica TM Gen2 PAM4 DSP for 800G Optical Module ApplicationsPart No.MV-CD822Product Type100G PAM4 DSPMarket SegmentsInside Data CentersApplications800G QSFP-DD/OSFPSingle-Mode Fiber TransceiversMulti-Mode Fiber TransceiversFeatures•8 x 100Gbps Optical PAM4 DSP Retimer •Support for 1x800G, 2x400G, 8x100G Ethernet traffic with breakout•5nm Low power•25% power savings enabling <12-Watt 800G•CMIS compliant with advanced diagnostic features•Integration of enhanced optical modulator driver DescriptionThe Marvell Spica Gen2 PAM4 DSP is a next generation solution for cloud data center, high-performance computing, and AI optical transceivers. It is an octal 100G/channel PAM4 DSP retimer that supports EML, silicon photonics and VCSEL applications.Spica Gen2 is manufactured with advanced 5nm process technology that delivers industry-leading power efficiency resulting in greater than 25% power savings compared to the previous generation of Spica PAM4 DSPs.The direct drive capabilities of the DSP combined with high performance receivers make Spica Gen2 ideal for 800GDR8/2xFR4/LR8 QSFP-DD/OSFP optical module applications.Highly integrated Spica Gen2 family of products minimize the components in the optical transceiver module and reduce overall system cost.Spica Gen2 also integrates advanced diagnostic features that make testing and building modules easy.Fully interoperable and compliant with the latest IEEE and CMIS standards, Spica Gen2 is optimized for high volume deployment within the data center.To deliver the data infrastructure technology that connects the world, we’re building solutions on the most powerful foundation: our partnerships with our customers. Trusted by the world’s leading technology companies for 25 years, we move, store, process and secure the world’s data with semiconductor solutions designed for our customers’ current needs and future ambitions. Through a process of deep collaboration and transparency, we’re ultimately changing the way tomorrow’s enterprise, c loud, automotive, and carrier architectures transform—for the better.Copyright © 2022 Marvell. All rights reserved. Marvell and the Marvell logo are trademarks of Marvell or its affiliates. Please visit for a completelist of Marvell trademarks. Other names and brands may be claimed as the property of others.Marvell_MV-CD822_PB Revised: 12/22。
【电子技术应用】_嵌入式系统设计_期刊发文热词逐年推荐_20140726
节能技术 节能意识 航迹仪 航向指示器 自适应阈值 自适应滤波 自适应backstepping滑模控制 自整角机 自恢复效应 联网设备 网络模型 网关 编解码器 编码/解码 纳斯达克 系统集成 系统模块 系统开销 系统开发 系统工程师 系统 管理设计 移液器 科技 神经网络 磁盘阵列 硬件实时操作系统 电量监测 电池非理想特性 电池组 电弧焊 电子差速技术 生物发酵 现场可编程门阵列 环境友好 片上可编程系统 温度控制 混合信号ic 混合信号 液晶屏 测试系统 永磁直线同步电机 模块 检测系统 检修机械臂 核酸提取 标签 条码识别 机械臂 机器人 有线网络 智能电话 智能变送器 显微图像处理
53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106
推荐指数 9 7 5 4 3 3 3 3 3 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
2009年 序号 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
科研热词 fpga 嵌入式 嵌入式系统 低功耗 嵌入式linux 单片机 μ c/os-ⅱ dsp arm 远程抄表 超低功耗 设计创新 解决方案 自动抄表 系统集成平台 电子元器件 模糊控制 无线通信 无线传感器网络 数字信号处理器 德州仪器 平台 市场发展趋势 嵌入式系统设计 多dsp嵌入式系统 可编程逻辑 可编程 卡尔 半导体 zigbee vxworks tms320dm642 tms320c6416t sopc msp430 linux arm7 高速缓存 高速公路 高性能 验证平台 非周期性 集成电路 速度控制 软件解码器 软件平台 超限报警 赛灵思公司 说话人识别 设计竞赛 设计应用 节能设计
迈瑞试剂日立上机参数
校准方法 Calib Type Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Linear Logit-Log(5p) Logit-Log(5p) Logit-Log(4p) Linear Linear
1浓度 0 0 0 0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0 0.00 0 0 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0
2浓度 # # # # # # # # # # # # # # # # # # # # #
# #
CK CKMB Ca Mg P α -AMY PA IgA IgG IgM C3 C4 CRP hs-CRP ADA
50 50
50 75 100 100 50 100 75 50 150 90 90 40 40 50 40 50 150 60 30 60 40
18800 11700 32000 32000 32000 32000 32000 32000 32000 32000 32000 32000 32000 32000 32000 32000 32000 0 32000 32000 32000 0 5000 32000 32000 32000 32000 32000 32000
2-2 2-2 2-2 2-2 2-2 2-2 6-3 6-3 6-3 6-3 6-3 6-3 6-3 5-3 2-2 2-2 2-2 6-3 2-2 2-2 2-2 2-2 2-2 2-2 6-3 2-2 2-2 2-2 2-2
PAN3512DK_Preliminary Spec_V01_B
VDDA VDDQ
VOLTAGE REFERENCE
BL BUTTON BM BR USB INTERFACE D+ DDEBOUNCE
Internal RC USB MOUSE CONTROLLER
Figure 3. Block Diagram
The PAN3512DK-TJZA supports X, Y, Z three axes, and L, R, M three buttons under USB mode. It is a CMOS process optical mouse sensor single chip with USB interface that serves as a non-mechanical motion estimation engine for implementing a computer mouse. The PAN3512DK-TJZA is in a 12-pin optical package and comes with the resolution of 1000 counts per inch (CPI) and the rate of motion up to 28 inches per second. It includes USB interface so that no mouse controller is needed to interface through USB. The PAN3512DK-TJZA can receive command and echo status or data format, both complete Universal Serial Bus® spec V1.1 and USB HID spec V1.11 compatibility. It is also a cost effective solution to support USB Mouse.
PUB10706 3A3771 型号多功能6口端子套件用户手册说明书
El kit contiene:Kit de terminales de 6 puertos, para múltiples cables, para usar en Protector Compacto de Circuito, 400 A, y en portafusibles CUBEFuse de 400 A. Número de catálogo CCP2-MW6-6Cubierta para terminalesTornillos de doble rosca(Hi-Lo)TerminalTornillo de montajeTornillo de placaPlaca antigiroFigura 1ContenidoDescripciónPág...........................1Instrucciones de ensamble (2)El kit contiene: 3 Terminales de 6 puertos 3 Placas antigiro6 Tornillos #6 para placa antigiro 3 Tornillos de montaje 1 Cubierta para terminales2 Tornillos de doble rosca (Hi-Lo) #4 x 1/2"1 Etiqueta de “Advertencia” e información del par de apriete 1 Placa de cubiertaRango de calibre del cable: 1/0-14 AWG (6 cables, máx.), cobre o aluminioEste kit está Listado UL (archivo E64983) para instalación en campo en el lado de carga del Protector Compacto de Circuito (CCP), de 400 A y en portafusibles CUBEFuse de 400 A.Nota: El propósito de estas terminales es distribuir alimentación a más de una carga. Deben instalarse en el lado de carga únicamente.CCP2-MW6-6Manual de instalación PUB107063A3771, Rev. BJulio de 202110 a 14 (6 a 2.5) 3.9 (35)8 (10)4.5 (40)4 a 6 (25 a 16)5.0 (45)1/0 a 2 (50 a 35)5.6 (50)Medio 1-3/8 a 1-1/2Inferior 2 a 2-1/8ADVERTENCIA:LOS CONDUCTORES DIMENSIONADOS PARA CORRIENTES DE CARGA INFERIORES A LA CLASIFICACIÓN DEL FUSIBLE NO ESTARÁN PROTEGIDOS POR EL FUSIBLE.CADA CABLE DE CARGA DEBE ESTAR PROTEGIDO POR UN DISPOSITIVO DE PROTECCIÓN CONTRA SOBRECORRIENTE, INDIVIDUAL, Y CUMPLIR CON LOS REQUISITOS ADICIONALES CORRESPONDIENTES DEL NEC.Instrucciones de ensambleSi el CCP o el portafusible CUBEFuse ya está instalado en el equipo, debe desmontarlo del equipo para instalar este kit. El kit está diseñado para usarse únicamente en el lado de carga. Vea la Figura 1.1. Quite y deseche las terminales para cable existentes en el lado de carga del CCP o del portafusible CUBEFuse, si es necesario.2. Coloque la placa antigiro en la terminal y asegúrela con los tornillos#6. Aplique un par de apriete de 0.6 N•m (5 lb-pulg).NOTA. Utilice únicamente los tornillos de montaje que vienen con el kit. No los sustituya, pues podría no cumplir con la separación eléctrica.3. Ensamble la terminal en la parte superior, como se muestra, y apriétela rmemente con el tornillo de montaje. Aplique un par de apriete de 22.0 N•m (200 lb-pulg).4. Use la placa de la cubierta (con sello de alambre únicamente).En este momento, el CCP o el portafusible CUBEFuse puedeinstalarse en el equipo. Después de instalarlo, coloque la cubierta de terminales con los tornillos de doble rosca (Hi-Lo). Vea la Figura 1.CABLEADO EN CAMPONota. Podría no ser posible instalar cables de mayor calibre en los puertos de terminales adyacentes debido al grosor del aislamiento del cable. Utilice únicamente conexiones quepermitan insertar los cables sin interferencia de aislamiento entre cables en la terminal. Cuando se inserta completamente en la terminal, el aislamiento del cable debe estar a menos de 1/8". Retire el aislamiento de los cables las longitudes que se indican a continuación.i K tC a n t i d a d 1C C P S E R I E B U S S M A N NC C P 2-M W 6-6P a r a i n t e r r u p t o r d e 400 A y p o r t a f u s i b l e C U B E F u s e d e 400 AT E R M I N A L E S P A R A M ÚL T I P L E S C A B L E SP A R A C C P D E 400 A Y P O R T A F U S I B L E C U B E F U S E D E 400 A ,S E R I E B U S S M A N NH e c h o e n E s t a d o s U n i d o sEaton y Bussmann son marcas comerciales de Eaton, registradas en Estado Unidos y otros países. No se permite el uso de las marcas comerciales de Eaton sin el previo consentimiento por escrito de Eaton.Estas instrucciones se publican únicamente con propósitos informativos y no deben considerarse como exhaustivas.Para mayor información, consulte a Ingeniería de Aplicaciones en el teléfono 800-8-387369, lunes a viernes, de 8:00 a.m. a 6:00 p.m., Hora del Centro, o envíe un correo electrónico a ********************La venta del producto que se presenta en este documento estásujeta a los términos y condiciones descritos en las políticas de venta correspondientes de la División Bussmann y otros acuerdoscontractuales entre las partes. Este documento no pretende ampliar ni agrega algún contrato de este tipo. El contrato entre el comprador y la División Bussmann es el único documento que rige los derechos del comprador de este equipo.LA INFORMACIÓN, LAS RECOMENDACIONES Y LASDESCRIPCIONES CONTENIDAS EN EL PRESENTE DOCUMENTO NO CONSTITUYEN GARANTÍA ALGUNA, EXPRESA O IMPLÍCITA, INCLUIDAS LAS GARANTÍAS DE IDONEIDAD PARA UNPROPÓSITO DETERMINADO O DE COMERCIABILIDAD, Y LAS GARANTÍAS QUE SURJAN DURANTE EL TRANSCURSO DE LA NEGOCIACIÓN O DE LOS USOS COMERCIALES.En ningún caso la División Bussmann será responsable ante elcomprador o usuario por contrato, por agravio (incluida la negligencia), por responsabilidad estricta o de otra manera por cualquier daño o pérdida especial, indirecta, incidental o consecuente, incluido pero no limitado a daño o pérdida de uso de equipo, instalaciones, planta o sistema de energía, costo de capital, pérdida de energía, gastos adicionales en el uso de instalaciones de energía existenteso reclamaciones contra el comprador o usuario por parte de susclientes como resultado del uso de la información, recomendaciones y descripción aquí contenidas.0517********Eaton1000 Eaton Boulevard Cleveland. OH 44122United States División Bussmann Poniente 148 núm. 933Industrial VallejoCiudad de México, 02300Eaton.mx/bussmannseries© 2021 EatonTodos los derechos son reservados.Impreso en México.Publicación Núm. PUB10706-spanish 3A3771Julio de 2021。
电源监控器手册说明书
Supply:Supply:Supply:Mounting Output Frequency 208 to 240 VAC 380 to 415 VAC 380 to 480 VAC DIN-rail 2 x SPDT 50 - 60 Hz DPC 71 D M23DPC 71 D M48Plug-in2x SPDT50 - 60 HzPPC 71 D M23PPC 71 D M48Product Description•TRMS 3-phase over and under voltage,phase sequence, phase loss, asymmetry and tolerance monitoring relay•Detect when all 3 phases are present and have the correct sequence•Detect if all the 3-phase-phase or phase-neutral voltages are within the set limits•Detect if asymmetry and tolerance are within the set value•Separately adjustable setpoints•Separately adjustable delay functions (0.1 to 30 s)•Output: 2 x 5 A relay SPDT NE•For mounting on DIN-rail in accordance withDIN/EN 50 022 (DPC71) or plug-in module (PPC71)•35.5 mm Euronorm housing (DPC71) or 35.5 mm plug-in module (PPC71)•LED indication for relays, alarm and power supply ONType Selection3-phase or 3-phase+neutral line voltage monitoring relay for phase sequence, phase loss, asymmetry, tolerance,over and under voltage (sep-arately adjustable set points)with built-in time delay func-tion.Supply ranges from 208 to 480 VAC covered by two multivoltage relays.DPC71PPC71Input SpecificationsOutput SpecificationsMonitoring RelaysTrue RMS 3-Phase, 3-Phase+N, Multifunction Types DPC71, PPC71DPC71, PPC71Supply SpecificationsGeneral SpecificationsMode of OperationAsymmetry definition.Asymmetry is an indicator of the mains quality and it is defined as the absolute val-ue of the max imum devia-tion among the mains volt-ages, divided by the nominal voltage of the 3-phase sys-tem. The definition changes according to the voltage ref-erence:1)in case of measuring phase-phase voltages:max |∆V PH-PH |V ∆NOM2)in case of measuring phase-neutral voltages:max |∆V PH-N |VNOMTolerance definition.T olerance is another indicator of the mains quality and it is definied as the absolute val-ue of the maximum deviation of the mains voltages from the nominal voltage, divided by the nominal voltage of the 3-phase system. The defini-tion changes according to the voltage reference:1)in case of measuring phase-phase voltages:max |V ∆NOM -V PH-PH |V ∆NOM2)in case of measuring phase-neutral voltages:max |VNOM -V PH-N |VNOMx 100x 100x 100x 100General Specifications (cont.)DPC71, PPC71Mode of Operation (cont.)Connected to the 3 phases (and neutral) DPC71 and PPC71 operate when all 3 phases are present at the same time and the phase sequence is correct. It can be decided whether to mon-itor upper and lower voltage level of each phase or their asymmetry and tolerance. Voltage level monitoring:if one or more phase-phase or phase-neutral voltage ex ceed the upper set level or drop below the lower set level, the red LED starts flashing 2 Hz and the respective output relay releases after the set time period.Asymmetry and tolerancemonitoring:if one or more phase-phaseor phase-neutral voltageexceed the set levels the redLED starts flashing 2 Hz andthe respective output relayreleases after the set timeperiod.Note:For both functions, ifthe phase sequence iswrong or one phase is lost,both output relays releaseimmediately. Only 200 msdelay occurs. The failure isindicated by the red LEDflashing 5 Hz during thealarm condition.Example 1(Mains monitoring - over andunder phase-phase voltage)The relay monitors over andunder voltage, phase lossand correct phasesequence.Example 2(Motor monitoring - startingand operating load - asym-metry and tolerance ofphase-neutral voltage)DPC71 and PPC71 ensurecorrect starting and operat-ing conditions. They monitorthe voltage level, phasesequence (correct directionof the motor rotation) andasymmetry.Frequent failures are fuseblowing and incorrect volt-age level. In case of fuseblowing the motor regener-ates a voltage in the inter-rupted phase. The relaydetects the failure andreacts due to e x cessiveimbalance among the phas-es.Function/Range/Level/Time SettingAdjust the input range set-ting the DIP-switches 3 and 4. Select the desired func-tion setting the DIP-switches 5and 6 as shown below. To access the DIP-switches open the plastic cover using a screwdriver as shown below.Upper knobs: Setting of upper ()and lower () level or setting of asymmetry (ASY) and toler-ance ( ) on relative scale. Lower knobs:Setting of delay on alarm times (DELAY 1, DELAY 2) on absolute scale:0.1 to 30s.DPC71, PPC71Operation DiagramsOver and undervoltage monitoring (2 x SPDT relays)Asymmetry and tolerance monitoring (2 x SPDT relays)DPC71, PPC71Operation Diagrams (cont.)Wiring DiagramsDPC71, PPC71 Dimensions。
超特克DN2535低阈值去极模式（常开）双极性MOS晶体管说明书
Supertex inc.DN2535L = Lot NumberYY = Year Sealed WW = Week Sealed= “Green” PackagingFeatures►High input impedance ►Low input capacitance ►Fast switching speeds ►Low on-resistance►Free from secondary breakdown►Low input and output leakageApplications►Normally-on switches ►Solid state relays ►Converters►Linear amplifiers►Constant current sources ►Power supply circuits►TelecomGeneral DescriptionThe Supertex DN2535 is a low threshold depletion mode (normally-on) transistor utilizing an advanced vertical DMOS structure and Supertex’s well-proven silicon-gate manufacturing process. This combination produces a device with the power handling capabilities of bipolar transistors and with the high input impedance and positive temperature coefficient inherent in MOS devices. Characteristic of all MOS structures, this device is free from thermal runaway and thermally-induced secondary breakdown.Supertex’s vertical DMOS FETs are ideally suited to a wide range of switching and amplifying applications where high breakdown voltage, high input impedance, low input capacitance, and fast switching speeds are desired.N-Channel Depletion-Mode Vertical DMOS FETsAbsolute Maximum Ratings are those values beyond which damage to the device may occur. Functional operation under these conditions is not implied. Continuous operation of the device at the absolute rating level may affect device reliability. All voltages are referenced to device ground.Pin ConfigurationProduct Marking3-Lead TO-2203-Lead TO-92YY = Year Sealed WW = Week Sealed= “Green” PackagingSi DN 2535 Y Y W W3-Lead TO-2203-Lead TO-92Package may or may not include the following marks: Si orPackage may or may not include the following marks: Si orGATESOURCEDRAINGATESOURCEDRAINDRAIN-G denotes a lead (Pb)-free / RoHS compliant package. Contact factory for Wafer / Die availablity.Devices in Wafer / Die form are lead (Pb)-free / RoHS compliant.† I D (continuous) is limited by max rated T j .ONotes:1. All D.C. parameters 100% tested at 25O C unless otherwise stated. (Pulse test: 300µs pulse, 2% duty cycle.)2. All A.C. parameters sample tested.Switching Waveforms and Test CircuitOUTPUTINPUT OUTPUT0VVDD0V-10VTypical Performance Curves0.50.40.30.20.1V GS = 1.0V0.5V 0V-0.5V-1.0V0.50.40.30.20.1I D (a m p e r e s )G F S (s e i m e n s )V DS (volts)t p (seconds)I D (a m p e r e s )Typical Performance Curves (cont.)1.101.051.000.950.900.8520015010050Q G (nanocoulombs)BV Variation with TemperatureB V D S S (n o r m a l i z e d )C (p i c o f a r a d s )I D (a m p e r e s )V DS (volts)JEDEC Registration TO-92.* This dimension is not specified in the JEDEC drawing.† This dimension differs from the JEDEC drawing.Drawings not to scale.Supertex Doc.#: DSPD-3TO92N3, Version E041009.Supertex inc. does not recommend the use of its products in life support applications, and will not knowingly sell them for use in such applications unless it receives an adequate “product liability indemnification insurance agreement.” Supertex inc. does not assume responsibility for use of devices described, and limits its liability to the replacement of the devices determined defective due to workmanship. No responsibility is assumed for possible omissions and inaccuracies. Circuitry and specifications are subject to change without notice. For the latest product specifications refer to the Supertex inc. (website: http//)©2013 Supertex inc. All rights reserved. Unauthorized use or reproduction is prohibited.Supertex inc.(The package drawing(s) in this data sheet may not reflect the most current specifications. For the latest package outline information go to /packaging.html .)JEDEC Registration TO-220, Variation AB, Issue K, April 2002.* This dimension is not specified in the JEDEC drawing.† This dimension differs from the JEDEC drawing.Drawings not to scale.Supertex Doc. #: DSPD-3TO220N5, Version C041009.View B。
CellTiter Glo Luminescent Cell Viability Assay Protocol
Promega Corporation ·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·1.Description (1)2.Product Components and Storage Conditions (4)3.Performing the CellTiter-Glo ®Assay (5)A.Reagent Preparation (5)B.Protocol for the Cell Viability Assay (6)C.Protocol for Generating an ATP Standard Curve (optional) (7)4.Appendix (7)A.Overview of the CellTiter-Glo ®Assay..............................................................7B.Additional Considerations..................................................................................8C.References............................................................................................................11D.Related Products. (12)1.DescriptionThe CellTiter-Glo ®Luminescent Cell Viability Assay (a–e)is a homogeneous method to determine the number of viable cells in culture based on quantitation of the ATP present, which signals the presence of metabolically active cells. The CellTiter-Glo ®Assay is designed for use with multiwell-plate formats, making it ideal for automated high-throughput screening (HTS) and cell proliferation and cytotoxicity assays. The homogeneous assay procedure (Figure 1) involves adding a single reagent (CellTiter-Glo ®Reagent) directly to cells cultured in serum-supplemented medium. Cell washing, removal of medium or multiple pipetting steps are not required.The homogeneous “add-mix-measure” format results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present (Figure 2).The amount of ATP is directly proportional to the number of cells present in culture in agreement with previous reports (1). The CellTiter-Glo ®Assay relies on the properties of a proprietary thermostable luciferase (Ultra-Glo™ Recombinant Luciferase), which generates a stable “glow-type” luminescent signal and improves performance across a wide range of assay conditions. The luciferase reaction for this assay is shown in Figure 3. The half-life of the luminescent signal resulting from this reaction is greater than five hours (Figure 4). This extended half-life eliminates the need for reagent injectors and provides flexibility for continuous or batch-mode processing of multiple plates. The unique homogeneous format reduces pipetting errors that may be introduced during the multiple steps required by other ATP-measurement methods.CellTiter-Glo ®Luminescent Cell Viability AssayAll technical literature is available on the Internet at: /protocols/ Please visit the web site to verify that you are using the most current version of this Technical Bulletin. Please contact Promega Technical Services if you have questions on useofthissystem.E-mail:********************Figure 1. Flow diagram showing preparation and use of CellTiter-Glo ®Reagent.Promega Corporation ·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·3170M A 12_0ACellTiter-Glo CellTiter-Glo MixerLuminometer®System Advantages•Homogeneous:“Add-mix-measure” format reduces the number of plate-handling steps to fewer than that required for similar ATP assays.•Fast:Data can be recorded 10 minutes after adding reagent.•Sensitive:Measures cells at numbers below the detection limits of standard colorimetric and fluorometric assays.•Flexible:Can be used with various multiwell formats. Data can be recorded by luminometer or CCD camera or imaging device.•Robust:Luminescent signal is very stable, with a half-life >5 hours,depending on cell type and culture medium used.•Able to Multiplex:Can be used with reporter gene assays or other cell-based assays from Promega (2,3).Figure 3. The luciferase reaction.Mono-oxygenation of luciferin is catalyzed byluciferase in the presence of Mg 2+, ATP and molecular oxygen.Promega Corporation ·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·3171M A 12_0A L u m i n e s c e n c e (R L U )Cells per Well10,00060,00020,00030,00040,00050,0000R² = 0.9990.5 × 1061.0 × 1061.5 × 1062.0 × 1062.5 × 1063.0 × 1063.5 × 1064.0 × 106r² = 0.99020,00010,00030,00040,00050,000r² = 0.9900100200300400HO SN S N O S N S N OCOOH +ATP+O 2Ultra-Glo™ Recombinant Luciferase +AMP+PP i +CO 2+LightBeetle Luciferin OxyluciferinMg 2+0Figure 2. Cell number correlates with luminescent output.A direct relationship exists between luminescence measured with the CellTiter-Glo ®Assay and the number of cells in culture over three orders of magnitude. Serial twofold dilutions of HEK293cells were made in a 96-well plate in DMEM with 10% FBS, and assays wereperformed as described in Section 3.B. Luminescence was recorded 10minutes after reagent addition using a GloMax ®-Multi+ Detection System. Values represent the mean ± S.D. of four replicates for each cell number. The luminescent signal from 50HEK293 cells is greater than three times the background signal from serum-supplemented medium without cells. There is a linear relationship (r 2= 0.99)between the luminescent signal and the number of cells from 0to 50,000 cells per well.Figure 4. Extended luminescent half-life allows high-throughput batchprocessing.Signal stability is shown for three common cell lines. HepG2 and BHK-21cells were grown and assayed in MEM containing 10% FBS, while CHO-K1 cells were grown and assayed in DME/F-12 containing 10% FBS. CHO-K1, BHK-21 and HepG2 cells, at 25,000 cells per well, were added to a 96-well plate. After an equal volume of CellTiter-Glo ®Reagent was added, plates were shaken and luminescence monitored over time with the plates held at 22°C. The half-lives of the luminescent signals for the CHO-K1, BHK-21 and HepG2 cells were approximately 5.4, 5.2 and5.8hours, respectively.2.Product Components and Storage ConditionsProduct Size Cat.#CellTiter-Glo ®Luminescent Cell Viability Assay 10ml G7570Substrate is sufficient for 100 assays at 100µl/assay in 96-well plates or 400 assays at 25µl/assay in 384-well plates. Includes:• 1 × 10mlCellTiter-Glo ®Buffer • 1 vial CellTiter-Glo ®Substrate (lyophilized)Product Size Cat.#CellTiter-Glo ®Luminescent Cell Viability Assay 10 × 10ml G7571Each vial of substrate is sufficient for 100 assays at 100µl/assay in 96-well plates or 400 assays at 25µl/assay in 384-well plates (1,000 to 4,000 total assays). Includes:•10 × 10mlCellTiter-Glo ®Buffer •10 vials CellTiter-Glo ®Substrate (lyophilized)Promega Corporation ·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·R e l a t i v e L u m i n e s c e n c e (%)Time (minutes)CHO-K101020304050607080901003173M A 12_0AProduct Size Cat.# CellTiter-Glo®Luminescent Cell Viability Assay100ml G7572 Substrate is sufficient for 1,000 assays at 100µl/assay in 96-well plates or 4,000assays at 25µl/assay in 384-well plates. Includes:•1 × 100ml CellTiter-Glo®Buffer• 1 vial CellTiter-Glo®Substrate (lyophilized)Product Size Cat.# CellTiter-Glo®Luminescent Cell Viability Assay10 × 100ml G7573Each vial of substrate is sufficient for 1,000 assays at 100µl/assay in 96-well plates or4,000 assays at 25µl/assay in 384-well plates (10,000to 40,000 total assays). Includes:•10 × 100ml CellTiter-Glo®Buffer•10 vials CellTiter-Glo®Substrate (lyophilized)Storage Conditions:For long-term storage, store the lyophilized CellTiter-Glo®Substrate and CellTiter-Glo®Buffer at –20°C. For frequent use, the CellTiter-Glo®Buffer can be stored at 4°C or room temperature for 48hours without loss of activity. See product label for expiration date information. ReconstitutedCellTiter-Glo®Reagent (Buffer plus Substrate) can be stored at room temperaturefor up to 8hours with <10% loss of activity, at 4°C for 48hours with ~5% lossof activity, at 4°C for 4days with ~20% loss of activity or at –20°C for 21weekswith ~3% loss of activity. The reagent is stable for up to ten freeze-thaw cycles,with less than 10% loss of activity.3.Performing the CellTiter-Glo®AssayMaterials to Be Supplied by the User•opaque-walled multiwell plates adequate for cell culture•multichannel pipette or automated pipetting station for reagent delivery•device (plate shaker) for mixing multiwell plates•luminometer, CCD camera or imaging device capable of reading multiwell plates •optional:ATP for use in generating a standard curve (Section 3.C)3.A.Reagent Preparation1.Thaw the CellTiter-Glo®Buffer, and equilibrate to room temperature priorto use. For convenience the CellTiter-Glo®Buffer may be thawed andstored at room temperature for up to 48hours prior to use.2.Equilibrate the lyophilized CellTiter-Glo®Substrate to room temperatureprior to use.Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·3.A.Reagent Preparation (continued)3.Transfer the appropriate volume (10ml for Cat.# G7570 and G7571, or 100mlfor Cat.# G7572 and G7573) of CellTiter-Glo ®Buffer into the amber bottlecontaining CellTiter-Glo ®Substrate to reconstitute the lyophilizedenzyme/substrate mixture. This forms the CellTiter-Glo ®Reagent.4.Mix by gently vortexing, swirling or inverting the contents to obtain ahomogeneous solution. The CellTiter-Glo ®Substrate should go intosolution easily in less than 1minute.3.B.Protocol for the Cell Viability AssayWe recommend that you perform a titration of your particular cells todetermine the optimal number and ensure that you are working within thelinear range of the CellTiter-Glo ®Assay. Figure 2 provides an example of sucha titration of HEK293 cells using 0 to 50,000 cells per well in a 96-well format.1.Prepare opaque-walled multiwell plates with mammalian cells in culturemedium, 100µl per well for 96-well plates or 25µl per well for 384-wellplates.Multiwell plates must be compatible with the luminometer used.2.Prepare control wells containing medium without cells to obtain a value forbackground luminescence.3.Add the test compound to experimental wells, and incubate according toculture protocol.4.Equilibrate the plate and its contents at room temperature forapproximately 30 minutes.5.Add a volume of CellTiter-Glo ®Reagent equal to the volume of cell culturemedium present in each well (e.g., add 100µl of reagent to 100µl of mediumcontaining cells for a 96-well plate, or add 25µl of reagent to 25µl ofmedium containing cells for a 384-well plate).6.Mix contents for 2 minutes on an orbital shaker to induce cell lysis.7.Allow the plate to incubate at room temperature for 10 minutes to stabilizeluminescent signal.Note:Uneven luminescent signal within standard plates can be caused bytemperature gradients, uneven seeding of cells or edge effects in multiwellplates.8.Record luminescence.Note:Instrument settings depend on the manufacturer. An integration timeof 0.25–1 second per well should serve as a guideline.Promega Corporation ·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·3.C.Protocol for Generating an ATP Standard Curve (optional)It is a good practice to generate a standard curve using the same plate onwhich samples are assayed. We recommend ATP disodium salt (Cat.# P1132,Sigma Cat.# A7699 or GE Healthcare Cat.# 27-1006). The ATP standard curveshould be generated immediately prior to adding the CellTiter-Glo®Reagentbecause endogenous ATPase enzymes found in sera may reduce ATP levels.1.Prepare 1µM ATP in culture medium (100µl of 1µM ATP solution contains10–10moles ATP).2.Prepare serial tenfold dilutions of ATP in culture medium (1µM to 10nM;100µl contains 10–10to 10–12moles of ATP).3.Prepare a multiwell plate with varying concentrations of ATP standard in100µl medium (25µl for a 384-well plate).4.Add a volume of CellTiter-Glo®Reagent equal to the volume of ATPstandard present in each well.5.Mix contents for 2 minutes on an orbital shaker.6.Allow the plate to incubate at room temperature for 10 minutes to stabilizethe luminescent signal.7.Record luminescence.4.Appendix4.A.Overview of the CellTiter-Glo®AssayThe assay system uses the properties of a proprietary thermostable luciferase toenable reaction conditions that generate a stable “glow-type” luminescentsignal while simultaneously inhibiting endogenous enzymes released duringcell lysis (e.g., ATPases). Release of ATPases will interfere with accurate ATPmeasurement. Historically, firefly luciferase purified from Photinus pyralis(LucPpy) has been used in reagents for ATP assays (1,4–7). However, it hasonly moderate stability in vitro and is sensitive to its chemical environment,including factors such as pH and detergents, limiting its usefulness fordeveloping a robust homogeneous ATP assay. Promega has successfullydeveloped a stable form of luciferase based on the gene from another firefly,Photuris pennsylvanica(LucPpe2), using an approach to select characteristics thatimprove performance in ATP assays. The unique characteristics of this mutant(LucPpe2m) enabled design of a homogeneous single-reagent-addition approachto perform ATP assays with cultured cells. Properties of the CellTiter-Glo®Reagent overcome the problems caused by factors, such as ATPases, thatinterfere with ATP measurement in cell extracts. The reagent is physicallyrobust and provides a sensitive and stable luminescent output.Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·4.A.Overview of the CellTiter-Glo®Assay (continued)Sensitivity and Linearity:The ATP-based detection of cells is more sensitivethan other methods (8–10). In experiments performed by Promega scientists,the luminescent signal from 50HEK293 cells is greater than three standarddeviations above the background signal from serum-supplemented mediumwithout cells. There is a linear relationship (r2= 0.99) between the luminescentsignal and the number of cells from 0 to 50,000 cells per well in the 96-wellformat. The luminescence values in Figure 2 were recorded after 10minutes ofincubation at room temperature to stabilize the luminescent signal as describedin Section3.B. Incubation of the same 96-well plate used in the experimentshown in Figure 2 for 360minutes at room temperature had little effect on therelationship between luminescent signal and number of cells (r2= 0.99).Speed:The homogeneous procedure to measure ATP using the CellTiter-Glo®Assay is quicker than other ATP assay methods that require multiple steps toextract ATP and measure luminescence. The CellTiter-Glo®Assay also is fasterthan other commonly used methods to measure the number of viable cells(such as MTT, alamarBlue®or Calcein-AM) that require prolonged incubationsteps to enable the cells’ metabolic machinery to convert indicator moleculesinto a detectable signal.4.B.Additional ConsiderationsTemperature:The intensity and decay rate of the luminescent signal from theCellTiter-Glo®Assay depends on the luciferase reaction rate. Environmentalfactors that affect the luciferase reaction rate will change the intensity andstability of the luminescent signal. Temperature is one factor that affects therate of this enzymatic assay and thus the light output. For consistent results,equilibrate assay plates to a constant temperature before performing the assay.Transferring eukaryotic cells from 37°C to room temperature has little effect onATP content (5). We have demonstrated that removing cultured cells from a37°C incubator and allowing them to equilibrate to 22°C for 1–2 hours hadlittle effect on ATP content. For batch-mode processing of multiple assayplates, take precautions to ensure complete temperature equilibration. Platesremoved from a 37°C incubator and placed in tall stacks at room temperaturewill require longer equilibration than plates arranged in a single layer.Insufficient equilibration may result in a temperature gradient effect betweenwells in the center and at the edge of the plates. The temperature gradientpattern also may depend on the position of the plate in the stack.Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·Chemicals:The chemical environment of the luciferase reaction affects theenzymatic rate and thus luminescence intensity. Differences in luminescenceintensity have been observed using different types of culture media and sera.The presence of phenol red in culture medium should have little impact onluminescence output. Assaying 0.1µM ATP in RPMI medium without phenolred resulted in ~5% increase in luminescence output (in relative light units[RLU]) compared to assays in RPMI containing the standard concentration ofphenol red, whereas assays in RPMI medium containing twice the normalconcentration of phenol red showed a ~2% decrease in luminescence.Solvents for the various test compounds may interfere with the luciferasereaction and thus the light output from the assay. Interference with theluciferase reaction can be detected by assaying a parallel set of control wellscontaining medium without cells. Dimethylsulfoxide (DMSO), commonly usedas a vehicle to solubilize organic chemicals, has been tested at finalconcentrations of up to 2% in the assay and only minimally affects light output.Plate Recommendations:We recommend using standard opaque-walledmultiwell plates suitable for luminescence measurements. Opaque-walledplates with clear bottoms to allow microscopic visualization of cells also maybe used; however, these plates will have diminished signal intensity andgreater cross talk between wells. Opaque white tape may be used to decreaseluminescence loss and cross talk.Cellular ATP Content:Different cell types have different amounts of ATP,and values reported for the ATP level in cells vary considerably (1,4,11–13).Factors that affect the ATP content of cells may affect the relationship betweencell number and luminescence. Anchorage-dependent cells that undergocontact inhibition at high densities may show a change in ATP content per cellat high densities, resulting in a nonlinear relationship between cell numberand luminescence. Factors that affect the cytoplasmic volume or physiology ofcells also will affect ATP content. For example, oxygen depletion is one factorknown to cause a rapid decrease in ATP (1).Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·4.B.Additional Considerations (continued)Mixing:Optimal assay performance is achieved when the CellTiter-Glo®Reagent is mixed completely with the cultured cells. Suspension cell lines (e.g., Jurkat cells) generally require less mixing to achieve lysis and extract ATP than adherent cells (e.g., L929 cells). Tests were done to evaluate the effect ofshaking the plate after adding the CellTiter-Glo® Reagent. Suspension cellscultured in multiwell plates showed only minor differences in light outputwhether or not the plates were shaken after adding the CellTiter-Glo®Reagent.Adherent cells are more difficult to lyse and show a substantial differencebetween shaken and nonshaken plates.Several additional parameters related to reagent mixing include the force ofdelivery of CellTiter-Glo®Reagent, sample volume and dimensions of the well.All of these factors may affect assay performance. The degree of reagent mixing required may be affected by the method used to add the CellTiter-Glo®Reagent to the assay plates. Automated pipetting devices using a greater or lesser force of fluid delivery may affect the degree of subsequent mixing required.Complete reagent mixing in 96-well plates should be achieved using orbitalplate shaking devices built into many luminometers and the recommended2-minute shaking time. Special electromagnetic shaking devices that use aradius smaller than the well diameter may be required to efficiently mixcontents of 384-well plates. The depth of medium and geometry of themultiwell plates may have an effect on mixing efficiency. We recommend that you take these factors into consideration when performing the assay andempirically determine whether a mixing step is necessary for the individualapplication.LuminometersFor highly sensitive luminometric assays, the luminometer model and settings greatly affect the quality of data obtained. Luminometers from differentmanufacturers will vary in sensitivities and dynamic ranges. We recommend the GloMax®products because these instruments do not require gainadjustments to achieve optimal sensitivity and dynamic range. Additionally, GloMax®instruments are preloaded with Promega protocols for ease of use.If you are not using a GloMax®luminometer, consult the operating manual for your luminometer to determine the optimal settings. The limits should beverified on each instrument before analysis of experimental samples. The assay should be linear in some portion of the detection range of the instrument used.For an individual luminometer there may be different gain settings. Werecommend that you optimize the gain settings.4.C.References1.Crouch, S.P. et al.(1993) The use of ATP bioluminescence as a measure of cellproliferation and cytotoxicity. J. Immunol. Methods160, 81–8.2.Farfan, A.et al.(2004) Multiplexing homogeneous cell-based assays. Cell Notes10, 2–5.3.Riss, T., Moravec, R. and Niles, A. (2005) Selecting cell-based assays for drugdiscovery screening. Cell Notes13, 16–21.4.Kangas, L., Grönroos, M. and Nieminen, A.L. (1984) Bioluminescence of cellular ATP:A new method for evaluating cytotoxic agents in vitro. Med. Biol.62, 338–43.5.Lundin, A. et al.(1986) Estimation of biomass in growing cell lines by adenosinetriphosphate assay.Methods Enzymol. 133, 27–42.6.Sevin, B.U. et al.(1988) Application of an ATP-bioluminescence assay in human tumorchemosensitivity testing. Gynecol. Oncol.31, 191–204.7.Gerhardt, R.T.et al.(1991) Characterization of in vitro chemosensitivity ofperioperative human ovarian malignancies by adenosine triphosphatechemosensitivity assay. Am. J. Obstet. Gynecol. 165, 245–55.8.Petty, R.D. et al.(1995) Comparison of MTT and ATP-based assays for themeasurement of viable cell number. J. Biolumin. Chemilumin.10, 29–34.9.Cree, I.A. et al.(1995) Methotrexate chemosensitivity by ATP luminescence in humanleukemia cell lines and in breast cancer primary cultures: Comparison of the TCA-100assay with a clonogenic assay. AntiCancer Drugs6, 398–404.10.Maehara, Y. et al.(1987) The ATP assay is more sensitive than the succinatedehydrogenase inhibition test for predicting cell viability. Eur. J. Cancer Clin. Oncol.23, 273–6.11.Stanley, P.E. (1986) Extraction of adenosine triphosphate from microbial and somaticcells. Methods Enzymol.133, 14–22.12.Beckers, B. et al.(1986) Application of intracellular ATP determination in lymphocytesfor HLA-typing. J. Biolumin. Chemilumin.1, 47–51.13.Andreotti, P.E. et al.(1995) Chemosensitivity testing of human tumors using amicroplate adenosine triphosphate luminescence assay: Clinical correlation forcisplatin resistance of ovarian carcinoma. Cancer Res. 55, 5276–82.4.D.Related ProductsCell Proliferation ProductsProduct Size Cat.# ApoLive-Glo™ Multiplex Assay10ml G6410 ApoTox-Glo™ Triplex Assay10ml G6320 CellTiter-Fluor™ Cell Viability Assay (fluorescent)10ml G6080 CellTiter-Blue®Cell Viability Assay (resazurin)20ml G8080 CellTiter 96®AQ ueous One SolutionCell Proliferation Assay (MTS, colorimetric)200 assays G3582 CellTiter 96®AQ ueous Non-RadioactiveCell Proliferation Assay (MTS, colorimetric)1,000 assays G5421 CellTiter 96®AQ ueous MTS Reagent Powder1g G1111 CellTiter 96®Non-RadioactiveCell Proliferation Assay (MTT, colorimetric)1,000 assays G4000 Additional sizes available.Cytotoxicity AssaysProduct Size Cat.# CytoTox-Glo™ Cytotoxicity Assay (luminescent)*10ml G9290Mitochondrial ToxGlo™ Assay*10ml G8000 MultiTox-Glo Multiplex Cytotoxicity Assay(luminescent, fluorescent)*10ml G9270 MultiTox-Fluor Multiplex Cytotoxicity Assay(fluorescent)*10ml G9200 CytoTox-Fluor™ Cytotoxicity Assay (fluorescent)*10ml G9260 CytoTox-ONE™ Homogeneous MembraneIntegrity Assay (LDH, fluorometric)*200–800 assays G7890 CytoTox-ONE™ Homogeneous MembraneIntegrity Assay, HTP1,000–4,000 assays G7892 CytoTox 96® Non-Radioactive Cytotoxicity Assay1,000 assays G1780 (LDH, colorimetric)*GSH-Glo™ Glutathione Assay10ml V691150ml V6912 GSH/GSSG-Glo™ Assay10ml V661150ml V6612 *Additional sizes available.LuminometersProduct Size Cat.# GloMax®-Multi+ Detection System with Instinct™ Software:Base Instrument with Shaking 1 each E8032 GloMax®-Multi+ Detection System with Instinct™ Software:Base Instrument with Heating and Shaking 1 each E9032 GloMax®-Multi+ Luminescence Module 1 each E8041Apoptosis ProductsProduct Size Cat.# Caspase-Glo®2 Assay*10ml G0940 Caspase-Glo®6 Assay*10ml G0970 Caspase-Glo®3/7 Assay* 2.5ml G8090 Caspase-Glo®8 Assay* 2.5ml G8200 Caspase-Glo®9 Assay* 2.5ml G8210Apo-ONE®Homogeneous Caspase-3/7 Assay1ml G7792 DeadEnd™ Fluorometric TUNEL System60 reactions G3250 DeadEnd™ Colorimetric TUNEL System20 reactions G7360Anti-ACTIVE®Caspase-3 pAb50µl G7481Anti-PARP p85 Fragment pAb50µl G7341Anti-pS473Akt pAb40µl G7441 Caspase Inhibitor Z-VAD-FMK, 20mM50µl G7231125µl G7232*Additional sizes available.(a)U.S. Pat. Nos. 6,602,677 and 7,241,584, European Pat. No. 1131441, Japanese Pat. Nos. 4537573 and 4520084 and other patents pending(b)U.S. Pat. No. 7,741,067, Japanese Pat. No. 4485470 and other patents pending.(c)U.S. Pat. No. 7,700,310, European Pat. No. 1546374 and other patents pending.(d)U.S. Pat. Nos 7,083,911, 7,452,663 and 7,732,128, European Pat. No. 1383914 and Japanese Pat. Nos. 4125600 and 4275715.(e)The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.© 2001–2012 Promega Corporation. All Rights Reserved.Anti-ACTIVE, Apo-ONE, Caspase-Glo, CellTiter 96, CellTiter-Blue, CellTiter-Glo, CytoTox 96 and GloMax are registered trademarks of Promega Corporation. ApoTox-Glo, ApoLive-Glo, CellTiter-Fluor, CytoTox-Fluor, CytoTox-Glo, CytoTox-ONE, DeadEnd, GSH-Glo, GSH/GSSG-Glo, Instinct, Mitochondrial ToxGlo and Ultra-Glo are trademarks of Promega Corporation. alamarBlue is a registered trademark of Trek Diagnostic Ssystems, Inc.Products may be covered by pending or issued patents or may have certain limitations. Please visit our Web site for more information.All prices and specifications are subject to change without prior notice.Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the most up-to-date information on Promega products.。