TaKaRa 1kbmarker使用说明书
TAKARA 实验室常规试剂配制方法
031*-2./4,+58679giaifi [`eg]\_debe^h \ZgZbe^edb`d]:mwwtYOOyyyNwioiuiNjsqNjr.1/{30,z +|2}-4*~Q c gunvM_\p Jt_WNTL WNVLXNPK:I JRNS7GDO :H:8b~:;:8:M JRNS QK :H 4^R U;8t 3L A99POg /}T8Yclw\1yU <8[?`~t 3-C9ix ;AD gQG O U=8u 1E j 2P :HU>8m >m B,{qY 7>]dUSF Z I61E |0P7>q N ijQG O Y H<JRNS 1E gQG O:>kg_ra G peY >k +5m:VY 1E gQG O e L vh989<nf =UQNU c gunvM_\pJt_XNXK:8>IJRNS7GDO :H:8b~:A:8@M JRNS QK :H 4^R U;8t 3L A99POg /}T8Yclw\1yU <8J-C9ixQG OP A8AU =8u 1E j 2P :HU>8m >m B,{qY 7>]dUSF Z I61E |0P7>q N ijQG OY H<JRNS1E gQG O:>kg_ra G peY >k +5m:VY 1E gQG O e L vh989<nf =UQP;g][xllku Jt_WNTL WNVL XNPK:99PI J RNS7GDO6:9PI FEJ B :H:8~.?*1E Y QK :H 4^R U;8@4^R t 3L A99POg /}T8Yz M soU <8u 1E j 2P :HqYm >m B,{U =87>]dUS c <EB Jt_UNRK031*-2./4,+5867:nrfrlr _ejnb`dijgjcq `^n^gjc jigeib;v ++}]WW ---V +rxr~rVs|zVs{k_m _,uut~57;SE FM@R062;SE C@R054SE FM 6BHG 806SE CB 6HG 85D52,8;;rr i m^5D h n p \62?h n p t b b <44SR B X4|z i /U{l _*\72Dt I H |y VB i F}k ;28i [ft bX4|z y _P G `k 5D\82]5]G F1f i q 5m :\yW j fx H?K?YOONW p7Mu K 4|i a DN i 2d K O p x /5SE @M@R 6b 429SE EP@R 6\YX h >KP54E 9|,544SR52,8;;25P 9|,m^544]644SR h n p i t b b 74SR B X4|z {l _*\62t X4|z y _P G `k 544SR\72n \42661S AR `A 31\82D W O3^q 5m :\yW j 9|,v\V U *i }T y H ]5]G F1\n~w *Ud`yJE<C52n \a :j <K ypu P h o T oV97d B i 7Dth >r 2\^U |i 9F l J \>]AP h o T-Ve d lj d i [q En \\/j O[q En \~-o T i ~-o T p Ed 5:4^J P,Bth >3X v a 0C L h*9i gAL h*92Z Q=|M /wBI <l@l JF>b AF>Bz 7C \Y 7io TB o 8J AF>[JF>B +W p 3tN i 6C 0v n \]o 8B o T ,B U |i 9F l J \62}~yW jo TZ k C p U iv 2Z Q It{e i}~j [=s *qPg /C \}]}~0[d.X2p ,B i _o T|4`B L Y {4[\<8zV :iv \S e b z :E i s \S 5\72o T N d j Y3d |C VB -x ;AF>U K ^]n >i Y 7n \o T T p E J o T ,B N d nP VB i ;A ;2<T f io T N d }~ON a ;j_P h o T [x :^164^i 7j B o T-~-z ~\8u _p W 1B o T t <d q 5;R m nP ;A q 5i [f d :<^z `p n o T/U ^*\`t b .m +*g<1IYQTURQTURh kr I H 425/\\hc 9o Qs i a r [JF>B B y 1Y Un rq +w4|B c -cr i /j Y P -j d i ]w^O r m t ]n >\a t b C ,oB 5E LWQX1B@RgVB<24Zi n\65{l R {l59U q i .m nP /UU ~f i 3Xf ~z >\b t [}~z u a\c t b C ,oB 425E LWQX1B@RgVB<24Zi n \65{l R {l59U q i .m nP /UU ~f i 3Xf ~z >\d t [}~z u ci g 2|?{U f ~z >\e n \VB r j Z]]n >B VB i <^;2<\f y o Tm^}d w 6O p 8^q ^m :\<C9GB9ML=SZ\]Z[]YT52{G j 8a |M M p 3X ?k o j ++n \o T 3@Q 3X85g69=68=5Z\@Q 2n ?k o s C v ]w^P>_]n >BU 4i L X85f]w^@30+`.p 1=B SJ \62K n ON j y LWQX1B@R N d o T _C ,oB @Q 3X85g68=5h kc 0c f i R b }d w 6O p 8^m :\YXR SpWoT mam031*-2./4,+58679aeZe_e TY^aVUX]^[^Wd USaS[^W^][Y]V:hrroRNNtttMrejepeMfnlMfmP ]]e^X*::A;7~}}HG~{x-0}HG .v P8LF ~}}R*}}HG 9y 4Y M T:A;71rj^M `xf 0V E u ~5[w 4Y J {PQ*{\-0E :A;7>?d N on 3b4YM VZnZqWQ +{a:A;7;/1rlV D.v P8p 1B e 2K ~}}HGQ ,{p 1BOCK9y 2+M lV 7<X_QPMQ ]XUk*{-:73G ~}}HG~{I /0{,HGc .v P8M n 3*~{.HGc *A:T ~~{.:PVs HmkQ *{7<X_QQ \]eUk-9:A3G ~8~{\-*1*{*E :A3G LF ~84Y M Vn 3G 0}}HGc .v P8lqW 1rQ *{n .v P8p 1B e 2K~8lV 6xg]>hQ +{i<i @}tlV,SX_QPOJ KcNbL Wksfnqg*}v w @|?x 6GOBJMD ~}}HG~{\-*}E 6GOBJMD LF ~}}R*}}HG 4Y M Vn 3G 0}HGc .v P8lVqW 1rQ *{n .v P8p 1B e 2K ~}}HGQ +{i <i @}tlV,SX_Q`nksrinm Q KG?DCEL*-H9>LFM z 73GTK70{}xy ~}H954>2y -}H96GOBJMD ~8~{x -=z 1B V LF ~84Y M Q*{i <i @}tlV,SX_Q+{5D,|-}HGc=JGONFJI RM n 3*HGc<:AMD 2T*}HE |HGPQ`nksrinm QQ KG?DCEL031*-2./4,+58679ce[eae TZ_cWUY^_\_Xd UScS\_X_^\Z^W:gppnRMMrrrLpeieoeLfmkLfl bmjqphml sssJG?DCEK{426-:u }4.1{.661}xx >=yw Md|fw\G 2.}xx >=rK 3Czw]q{xx >=mc 5x[_IoaC {w ]mc 5x^o +Up 1}xx >=C |w X{X *hb[G |EJQCNLP ]WVcS JnYQLNKxw}40/8-y 3ywMl y +~wy ;59z 0/8-D z 1z 6G 2.y 3rK 3C zw ]q /+xx >=Smc 5xGOV_IC {w -5961T`?101+wx F /zx ;5961BC 4,H?101+wx sG0/8-LjznoaC|w ]mc 5x^o +Up 1y 3C }w udVN}W[GX{X *hbC ~w v{JQCO ]Vccy 4/88zx >=yw Ml {wx ,;/88G]qR }x >=yec~YiCzw ]zx >=S xwxy 4596-:F?1}wz BGoa[t -xwzz 6>gkZgPbC {w udVN}W[G vzx EJQCON k]Sc`yx >4-87zx >=yw Ml yzy >;59z -87D {1z 6G]qR }x >=yec~YiC zw ]zx >=S z}>48@<A v 1.=F?1+wx BG_IoaC {w udVN}W[G vzx EJQC031*-2./4,+58679jncnhn \bfj_]aefdf`m ][j[df`fedbe_:s}}zZTT ***S}nun{nSoywSox /-=1;9486,5.:07<32WUO PlTkQ [o{+vnwtpqFC?eVBUfLX`b]W^\YZ D QDA p :[;O.g ie D Q I l l cEA \I l l/Gf ICC ^]v D9nR grz 1j E2cFA /D9nR0E_x Fh D Qg c CAGH r ^<B<Dgk c GA eoH@l Gdkscm`hm Zq i 6d+C v K4y ]gn 7T }?|YP g Apc6d-8]g >jMb t QS wc 5m Zq i X ygu ^b3L o p g aUd7=~dJ:v V5*q{cXUO PlTkQ [o{+vnwtpqGC?eVBUfLX`b]W^\YZ D QDA p :[;O.g ie D Q I l l cEA \I l l/Gf ICC ^]v D9nR grz 1j E2cFA /D9nR0E_x Fh D Qg c CAGH r ^<B<Dgk c GA eoH@l Gdkscm`hm Zq i 6d+C v K4y ]gn 7T }?|YP g Apc6d-8]g >jMb t QS wc 5m Zq i X ygu ^b3L o p g aUd7=~dJ:v V5*q{cVUO PlTkQBEHMY;j{t|R`v +otxq \~rrq{Pi^iRg[`_=JC<IQCADEH R T`\a@DAEH R O]bX\_Z@CAH?eVBUfSNS D QDA p :[;O.g ie D Q I l l cEA /Gf JCC ^]v D9nR g 1j E2cFA /D9nR0E_x Fh D Q ,g NW ksc031*-2./4,+58679Z\S\X\LRVZOMQUVTVP[MKZKTVPVUTRUO:bjjhJHHlllGj\d\i\G]geG]fI;YNYFWKPO Tg\^cfaLk``_ix ;:tq mu 3p *`F C=ts ;:.ok 5[E Cuq avk G,xe]Uy B x ;:Cvq 4XD xss J :r XEW F ]F =~0IRC wq }:tc ux J :T up ,~aSs 4X E Cxq az up ,~T+=459<8{A776?i @~03IR 7Z >H<Csqtn D3r 2Egr -nj/puxso uxn D2r 2E 9LP o tsn D2r 2E KQ /t +tq Mu t 8gr -nj/puxs F C=t +|J E Cuq mu uxs ;:T 9LPaz{+|J E FdHxeC vq az tss ;:TKQ /FdHf ?C wq az yxs ;:Tvk G,FdHf ?C xq :q A Ovhl 2D ]F~0IRCtsn D2r 2EQ /o xn D2r 2E 8P t +tq mu 3px 6F C=t +|J E Cuq NW^\]}:C031*-2./4,+58679TVOVSV INRTKJMQRPRLU JHTHPRLRQPNQK:X__]GFF```E_VYV^VEW\ZEW[fdjb~tes *nwqr i c gb~ses *J qo i |o h rc fdfjb~tes *quro gff yxgd EM^FW?+@S gff}hff yx 5W?Lj {hd Cf>;{9Qac[]TZHk {R,XJ8:6UQa=2>lk+3Uc/@J,X z ghZH {id .PTac_d_K+0b-4{fdffkb~tes *IGY c fdfhb~ses *AO g pgd 1E^FW?+ie g p W?Lj {hd @S g p NDmX<+`7>;QB {id V\-4{031*-2./4,+58679_aWa]a QV[_SRUZ[X[T`RP_PX[T[ZXVZS:elljOIInnnHlafakaHbigHbhMJ;_PS QmddckFjUNHMGv .3;7<q Q 1p utt 9.+*3}u -ur Mo 6p .dB JC u -~G L >vr 7~G L e|D |tt 98Uxm P0BNZhEzj>wr e|y{ru 98UQ 1BNZhE>xr exm P0gz =X{H u -`B -4FR>[`WcaZ_]T\UbV^dYXKJ;_QSQmddckFjUNHLGKJ;Y[\^Qmddckvtt 9..012p vt 9./40}5p ut 9.+*3}u -ur Mo xur|6.012B JC u -~G L >vr e D {tt 98*+1~Pn 0BhEzj>wr +B v //40,Vi:,GH {rt >xr F7z =L e|6p .d>yr B *+1~Pn 0gz =X{H u ->zr B trxy e 9qs^qOx EK >{r -4I]FR>N Cz =f]c[4rk`bJa>Ja *<l +B BSJ_*K ;+B>ut 96s 98S@W utt 98ur Mo u 6S@WBe|T utt 98{w L >vr e|xm P0utt 98BNZhE /8*2yzj S@W>wr gz =O?HQ }v L B -4I]FR>xr S@WU\R tY 3try e 6s 98>NA C S@W ,>MI D 5K BH :89L R >031*-2./4,+5867:npipmp bhlnecgkljlfo canajlf lkjhke;w **}`\\,,,[*pyp~p[r|z[r{52g d {^-.PL d R Q -H y J a 4r z 429_G>A K 5_G=A[]62<sd R^LdRe2c m o u ^l ].7c Ov{+-lC h f ]72Ov K 0^-.PHy J a oJ1^n Lt ?lC h-94.t^[]k d QU .P -H yJ B QUd R-H y J k F5K ]82XlC h-hY8w i =c cl X cw [A@X c pD X8m `f OqrFl ].]Oq?w f \+s J 50D c k*w3q~/c @BG 1lC h c yl ].x 6V W r F ]{o qj :+|c _a l ].7mrF ]k FkN c X8m `f OqxP n L ?sc a |+s J if50x 3`Oq c 9Z I O is J ?q j 5c Y v l ].dRe 2n m c I I -G 8m `]rFl ].x ck F i \l ].x 67mrF c 9Z c I Y v .P6>bE n j ]92ys J Zn a:4`pTc u EH W X{xOvrM /s J a h e2429t N3QP[clx 6J W ]k d rM /z Kib e;e ]y Q @SrM /-s J x c k*A~/]:2Ql ].s J ,v d Rb f c pD X {+9c zq w *n ]dRC2K f X 7X 9QQ ^P ];2X |:<yRd=a~V 746g X 5@x [c pD4cU .P p f U D .P ]k d dR n ]My Q x c k Q i =cQdR h AD X 8`<i}c K f W i}6^92]_<j|psx{v b +uut~Ydka?QRZ^]<j|psx{v b +uut~Ymka?QRZ54QP52u ^<_}N c cU 54QP [B >f ]62?[B >f Ov V 8QP -@AF?z \,D cx 6S g sT ]72Ov 9QP -;R aCPWLMUSPb D cx 6J W ]82Q @AF?z \,0t a 54QP D c |:i}]031*-2./4,+58679^`W`\`PVZ^SQUYZXZT_QO^OXZTZYXVYS:emmjNJJoooIm`f`k`IaigIahLK;]]Qytv 23:,@r vty 2olzn w 1wt Rg|hx_N 0,w 1vQ 1Ixt }vQ 1`u -~vv A@Vrf 3yNTZbOsdIyt W`wz 30,@NXcD0./}tv ^N`rf 3yas *Yt /w 1I zt \{\~me^Nw{PUI<B475HF?EC;A>4=6D8@G:9LK;]][SPnddckytv 23:,@r vtx 23:0x 54z r vtvx 2.-7*w 1wt Rg|hx_N 0,w 1vQ 1Ixt }vQ 1`u -~vv A@Vrf 3yNTZbOsdI yt `3:40XcD0./}tz L -|t{A@V wv 33:40HI zt `rf 3yas *Yt /w 1I {t\{\~me^Nw{PUIMK;Rche`kbmHlCD{vv A@wt Rg|hx_N 0,{vv A@vQ 1Ixt `rf 3y -zvv A@NTZbOsd yt `rf 3yas *Yt /{vv A@Izt +vtz{I Ajq]j^NZ 2Sk[x{A@I{t sxv KPUIvt{23:x 054z w 1wt Rg wyz >3:x 054z J }0x 40,w 1vQ 1I xt `u -~vv A@Vrf 3yTZbOsdIyt `u ~{q V0y 54z LpizMXcs *D0./}tx I zt `rf 3yYt /w 1I{t \{\~me^Nw{PUI031*-2./4,+59687:UWPWTW JOSULKNRSQSMV KIUIQSMSRQORL;Y``^HGGaaaF`WZW_WFX][FX\kj 62i 65*.5687w|t N kjj 65kh M}^K n w|t l 2UJ ojj 65.G W Dj -N ljj 65V+x u <1109>lh I RrkEz 3=kE l ?n B0D ,lcj -n 65V o {|.v 5D 1y Y wY <jhk {>mh I I]bI]i u[`P 9k S>nh d 56A,F cD 1I kq ax @[/R;o 7>U ,FN lj SD H |Z w|t >oh j -l H :gV M p G Qm DG QLW>ph q C d 5w|t cD ,l J kjj 65V~q ]6W DKX ,F V }w lpj S >qh i 8,F V w|t wYcD ?2w|t ,FT kj 62i 65>rh Z \kj ^X cD^\N B _B k 65i _=>glj AFT>sh 1I {Q \6LW j +o ^X cD E7J L p *>kjj 65kh Ms @t 4hD UJ ljj 65.G W >lh O^e O cD 1I jhno _6vzv~PVc 1I >kjj 65kh Ms @t 4hD UJ ljj 65.G W >lh O^e O cD 1I jhno _6vzv~PVc 1I >031*-2./4,+586779TVOVSV INRTKJMQRPRLU JHTHPRLRQPNQK:X__]GFF```E_VYV^VEW\ZEW[lr 1y v 08+/2,e mq 1y |4/5e igilpb c~h}d {u{e kib c}h}d UG j xjg EZh[dS?sn j x bBt;kg jbBtTao oii 10K^Yue?FMW@_X;lg T^YueV_kL`r qii 10Q?Ta kii 10KUG;mgcgAH;ki 1y |4/5fwt 0e jni 1y z *t 0e iginb =}h}>|7,,2ki j xjg EZh[dS?sn j x bBt;lg Ta ign 10|7,,2ki QFMRp;mg T^YueV_kL`r j x Q?m<AH;nb =~h}>fq\N h|s{|s 6--,4jii 10jg EZ n .fq\NTaJ jii 10K |s{|s 6--,4t?FMW@_X;kg m <AHIm=CODklPi]im:;031*-2./4,+586779\_V_[_PUY\RQTXYWYS^QO\OWYSYXWUXR:bkkiNIIlllHk_d_j_H`hfH`gOfic`ceecg EKJJfaIfeFrqq 74p 76z 7:5256658vq 76ro Jf v 4z 7:5256658-}vq 76eyU .Aso ]q uq 76icuEKRZXoaYEQp ,vq 76A to |qoss 17hkWhLcAuo xSR /C r 76p SDYE nsq BGMAUZ\SELMfaIfeFsu 74p 76*./}vq 76ro Jf ros 4*./}-}vq 76eyU .Aso ]q uq 76icuEKRZXoaYEQp ,vq 76A to |qoss 17hkWhLcAuo xSR /C r 76p SDYE nsq BGMA]GS_eELJfaIfeFnsqBIVGMAr +ro Jlwgt\E -}r +rH .Aso ]q~yqq 76Nme 0uEKR_FoaA to O]v ,,1-~C~qos 76@EP`:~+,xoq A uo ]me 0u^j{[Qp ,r +A vo TvTzicYEdn ,svAwo ]q r 76z 7:5256658C rqq 74p 76DYb *ZXA xo u BGMA031*-2./4,+59678:UWPWTW JOSULKNRSQSMV KIUIQSMSRQORL;Y``^HGGaaaF`WZW_WFX][FX\i xif ]wZjnOFp>hfio y wv j |{l @hfoj y w j v|{l ?ihh 21<aU jfki /wv j |{l M ijfml /w j v|{l r qh 21I_Xyix@TAaUN@R_Xy iJbu ihh 21@LkLm[V<jf D^lYgQ@vr i x eCx<kf Rcs phh 21I_Xyi@EKTAaU<lf R_XyiS\oPJbu i x N@LkLm[V<mf HapW`u nh =qlf@Rc ihh 21Idh[VZjnOFp<nf l =BG<i xif ]w jmh 2y ws 1ap<t qh 21I_XyixaU ifpn /ws 1N@Jbu ihh 21<jf ]w j y y /s 1j ap<t qh 21_XyixaU iq /y /s 1j N@Jbu ihh 21@LkLm[V<kf D^lYgQ@vri x eCx<mf HapW`u nh =qlf@Rc ihh 21Idh[VZjnOFpM i 21I r 250-01103dihh 2/g 21e <031*-2./4,+59678:UWPWTW JOSULKNRSQSMV KIUIQSMSRQORL;Y``^HGGaaaF`WZW_WFX][FX\gff 21gd az g w bnmgs<V gn /bnmgu of 21f\|l{AFNgYRMhy gff 21At fdhh }2^cP^G[<hd p gff 21zyq `rT{UiG[J g w bnmgs h 21AZwSQ<id j >DH<jd Uiv nff 21Jf\|lAFNWCgY<kd ]e gf 21hkf 2w ur 1gsAUiIjE{<ld KU k x x +yt gs?v fdh 21;ALX5t xy mdf <md Uif\|lV`rTMhy g v <nd OoOq_[RA j >DH<od ktdUi k 21_[J h w w /r 1hgs<g v031*-2./4,+59678:UWPWTW JOSULKNRSQSMV KIUIQSMSRQORL;Y``^HGGaaaF`WZW_WFX][FX\x ,+w .Y rXRk q -9-4256p .2/D}]KHr H E P F 2I Uh `x ,q +w .Y r UO@x ,w .Y rXRk+0-9:r ;:8-.:Dw -M6S E P F 2I Uh `x ,+w .Y r UO@he I d[N.Y r /f m Pl [N.Y rF c i Q i X,{4Ft =T+D s k ^\l @ie i Q i X,{oF[?EL6W .Y rF Z d ;3C5e q 5eJ Vgp b DY B.Y r Q eniF VWK>>@je \.Y r~8g lg B mg C B Ft =9R R c Sj D <|AG _>F Z d ;3Vgp b@h vie t =a ogg 43^7*nF FVgvM :y@je `t l x x -yt D a gei 43>Fax7t fg neg @ke t 7*nF u .Y rc ;g h v oFt =hl 1p 1-8@le i Q i X,{oF~8g mg C o^@me t =h 43p 472.23325D hgg 41f 43>?h 43uz|s D ik 41f 43>?i 43*ds -3D ig 41f 43E oz b pm@oe k CQjNZ@he T 6T+D sF h_h v @O k @ie t =a ogg 43^7*nF FVgvM :y@je `t l x x -yt D a gei 43>Fax7t fg neg @ke t 7*nF u .Y rc ;g h v @le i Q i X,{oF k CNZ@031*-2./4,+59678:UWPWTW JOSULKNRSQSMV KIUIQSMSRQORL;Y``^HGGaaaF`WZW_WFX][FX\h whe c|`osSIu@gehn x vt i zy k B geni x v i tzy k A hgg 10>gZ iejh .vt i zy k P hielk .v i tzy k w pg 10Ke^~n}BYCgZRBWe^~nLhz hgg 10BNpNra\>ie Gdq_lVB{w h w kE}>je Wix ogg 10Ke^~nBHMYCgZ>ke We^~nXbtULhz h w RBWi hl .q .+5>le NpNra\RB]fz mg ?*v>me Wi hgg 10Kjma\`osSIu=h 10q 14/,/00/2@hgg 1.f 10<=h 10uz{s @ik 1.f 10<=i 10~ds +0@ig 1.f 10AR[yTQ>oe k ?FODJ>。
RNA marker 1000
制品内容
RNA Marker RL1,000 6×Loading Buffer DEPC 处理水
20 μl 100 μl
1 ml
使用方法 A. 普通琼脂糖凝胶电泳时
1. 按下列组份配制 RNA Marker 样品。
RNA Marker RL1,000 6×Loading Buffer DEPC 处理水
RNA Marker RL1,000
使用说明书
Takara Code : D508A
包 装 量 : 约 20 次量
浓 度 : 约 200 ng/μl
保 存 : -80℃（短期保存可放置于-20℃）
制品说明 RNA Marker RL1,000是由体外转录得到的8条高纯度的单链RNA片 段组成的，其长度分别为1,000、800、600、500、400、300、200 和100 Bases，每微升本制品的RNA量约为200 ng。可用于RNA的 琼脂糖变性凝胶电泳（甲醛或乙二醛）或者普通的RNA琼脂糖凝胶 电泳等。每次取1 μl电泳时可使用约20次。
4. 甲醛变性琼脂糖凝胶配制方法如下： 加 6.0 g 高质量的琼脂糖到 93 ml DEPC 水中，煮沸直至完全溶 解，再加入适量的 DEPC 水定容至 93 ml。溶液冷却至 60℃左 右后，加入 30 ml 5×MOPS-EDTA Buffer 和 27 ml 37%甲醛（在 通风橱中操作），倒胶后于室温静置 1 小时。
1 μl 2 μl up to 10 μl
2. 均匀混合后 65℃加热 10 分钟，迅速冷却至室温（最好用 PCR 仪）。
3. 使用高质量的琼脂糖，用 1×TAE Buffer 制备 5%凝胶，制胶时 凝胶中请加入溴乙锭（终浓度：1 μg/ml）。
marker说明书
CERTIFICATE OF ANALYSISPageRuler ™Prestained Protein Ladder#SM067210 x 250 µl(for 100 mini gel applications 5 µl per well or 50 large gel applications 10 µl per well)Lot: Expiry Date:Storage: stable at 4°C for up to 3 months. For long term storage, store at -20°C.SM067_57_9.docDescriptionPageRuler ™Prestained Protein Ladder is a mixture of 10 recombinant, highly purified colored proteins with apparent molecular weights of 10 kDa to 170 kDa. Ladder proteins are covalently coupled with a blue dye except for two reference bands prestained with different colors. The 72 kDa reference band is orange and 10 kDa reference band is green.The ladder is supplied in gel loading buffer and isready-to-use: no heating, further dilution or addition of a reducing agent is required.ContentsApproximately 0.1-0.2 mg/ml of each protein in the storage buffer (62.5 mM Tris-H 3PO 4 (pH 7.5 at 25°C), 1 mM EDTA, 2% (w/v) SDS, 10 mM DTT, 1 mM NaN 3 and 33% (v/v) glycerol).Applications∙ Monitoring of protein separation during SDS-PAGE (1). ∙ Verifying Western transfer efficiency (2, 3).∙ Approximate sizing of proteins on SDS-polyacrylamidegels and Western blots.Instruction for Use❶ Thaw the ladder at room temperature for a few minutes to dissolve precipitated solids. DO NOT BOIL!❷ Mix gently, but thoroughly, to ensure the solution is homogeneous.❸ Load the following volumes of the ladder on an SDS-polyacrylamide gel:– 5 µl per well for mini gel,– 10 µl per well for large gel.Use the same volumes for Western blotting.❹ After the run is complete, stain the gel or perform Western transfer procedure as desired.Note•Each lot of the PageRuler™ Prestained Protein Ladder is calibrated against a precisely sized, PageRuler™ Unstained Protein Ladder and calculated apparent molecular weights are reported in the picture.•For precise molecular weight determinations use PageRuler™ Unstained Protein Ladder, #SM0661, see.•In 8 or 10% gels low molecular weight proteins may migrate with the dye front.•Loading volumes are intended for use in gels with a thickness of 0.75 mm. For thicker gels, the recommended loading volume should be increased.•PageRuler™ Prestained Protein Ladder could be used in Western blotting with all common membranes: PVDF, nylon and nitrocellulose.•Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100 kDa) proteins.Lot specific MW, kDa4-20% Tris-glycine SDS-PAGEcontinued on back pageQUALITY CONTROL5 µl of PageRuler™ Prestained Protein Ladder resolves 10 bands of equal intensities in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting onto PVDF membrane.Quality authorized by: Jurgita Zilinskiene References1. Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680-685, 1970.2. Burnette, W.N., "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate – polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A, Anal. Biochem., 112 (2), 195-203, 1981.3. Towbin, H., et al., E lectrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications, Proc. Natl. Acad. Sci. USA, 76, 4350-4354, 1979.This product is manufactured under the license forStrep-tag® technology covered by US patents Nos.5,506,121, 6,103,493 and foreign counterparts. Related Products∙DualColor™ Protein Loading Buffer Pack #R1011∙Loading Buffer Pack #R0891∙Spectra™ Multicolor Broad Range Protein Ladder #SM1841∙PageRuler™ Unstained #SM0661∙PageRuler™ Plus Prestained Protein Ladder #SM1811∙PageSilver™ Silver Staining Kit #K0681∙PageBlue™ Protein Staining Solution #R0571∙10X Tris-glycine-SDS Buffer #B46∙10X Tris-tricine-SDS Buffer #B48∙DTT #R0861 ∙ProteoJET™ Mammalian Cell Lysis Reagent #K0301∙ProteoJET™ Cytoplasmic and Nuclear ProteinExtraction Kit #K0311∙Bradford Reagent, ready-to-use #R1271∙Bovine Serum Albumin Standard Set, ready-to-use #R1281∙Bovine Gamma Globulin Standard Set, ready-to-use #R1291PRODUCT USE LIMITATION.This product is developed, designed and sold exclusively for research purposes andin vitro use only. The product was not tested for use in diagnostics or for drugdevelopment, nor is it suitable for administration to humans or animals.Please refer to for Material Safety Data Sheet of the product.。
蛋白Marker说明书
蛋⽩Marker说明书PageRuler Prestained Protein LadderSM0671A prestained SDS-PAGE MW marker with contrasting colored reference bands at10 and 70kDa.The Thermo Scientific PageRuler Prestained Proteis a mixture of ten (10) blue-, orange- and green-srecombinant proteins (10 to 170kDa) for use as sizstandards in protein electrophoresis (SDS-PAGE)Western blotting.This prestained protein MW marker is designed fomonitoring the progress of SDS-polyacrylamide geelectrophoresis, for assessing transfer efficiency onylon and nitrocellulose membranes, and for estimapproximate size of separated proteins that have b visible with gel stains or Western blot detection reagents. The ladder contains one orange refer at 70kDa and one green band at 10kDa.Highlights:Size range– 10 proteins spanning 10 to 170kDaReady-to-use– supplied in a loading buffer for direct loading on gels; no need to boil Sharp bands– color-coded bands of similar intensity for easy visualizationQuality tested – each lot evaluated by SDS-PAGE and Western blottingTwo reference bands– orange at 70kDa and green at 10kDaMembrane-compatible– colored bands transfer to membranes for Western blotting Includes:Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 1 1mM NaN3 and 33% glycerol. Applications:Monitoring protein migration during SDS-polyacrylamide gel electrophoresisMonitoring protein transfer onto membranes after Western blottingSm1841The Thermo Scientific Spectra Multicolor Broad RangeProtein Ladder is a 4-color protein standard containing 10prestained recombinant prokaryotic proteins (10 to 260kDa)for use as size standards in gel electrophoresis and Westernblotting.This prestained protein MW marker is designed formonitoring the progress of SDS-polyacrylamide gelelectrophoresis, for assessing transfer efficiency onto PVDF,nylon and nitrocellulose membranes, and for estimating theapproximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Four different chromophores (blue, orange, green, pink) are bound to the different component proteins, producing a brightly colored ladder with an easy-to-remember pattern.Highlights:Size range– 10 proteins spanning 10 to 260kDaMulticolor– four different colors for unambiguous band-size assignmentReady-to-use– supplied in a loading buffer for direct loading on gels; no need to boilSharp bands– color-coded bands of similar intensity for easy visualizationQuality tested – each lot evaluated by SDS-PAGE and Western blottingMembrane-compatible– colored bands transfer to membranes for Western blotting Includes:Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.Applications:Monitoring protein migration during SDS-polyacrylamide gel electrophoresisMonitoring protein transfer onto membranes after Western blottingSizing of proteins on SDS-polyacrylamide gels and Western blotsProduct Details:Sm1811The Thermo Scientific PageRuler Plus Prestained ProteinLadder is a mixture of nine (9) blue-, orange- andgreen-stained recombinant proteins (10 to 250kDa) for useas size standards in protein electrophoresis (SDS-PAGE)and Western blotting.This prestained protein MW marker is designed formonitoring the progress of SDS-polyacrylamide gelelectrophoresis, for assessing transfer efficiency onto PVDF,nylon and nitrocellulose membranes, and for estimating theapproximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. A blue chromophore is bound to all proteins, except proteins of two reference bands of 70kDa and 25kDa that are colored with an orange dye and one green reference band of 10kDa. PageRuler Plus Prestained Protein Ladder is ready to use: no heating, further dilution or addition of a reducing agent is required before loading onto a gel.Highlights:Size range– nine proteins spanning 10 to 250kDaReady-to-use– supplied in a loading buffer for direct loading on gels; no need to boilSharp bands– color-coded bands of similar intesity for easy visualizationQuality tested – each lot evaluated by SDS-PAGE and Western blottingBright reference bands– orange at 70 and 25kDa, and green at 10kDaMembrane-compatible– colored bands transfer to membranes for Western blotting Includes:Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.Applications:Monitoring protein migration during SDS-polyacrylamide gel electrophoresisMonitoring protein transfer onto membranes after Western blottingSizing of proteins on SDS-polyacrylamide gels and Western blotsProduct Details:。
λ-EcoT14Ⅰ说明书
λ-Eco T14Ⅰdigest DNA Marker
使 用 说 明 书
TaKaRa Code ：D3401A
●包 装 量：50μg（500μl×2支；内含1×Loading Buffer）
●浓 度：50 ng/μl
●制品说明：λ-Eco T14 I digest DNA Marker是由Bacteriophage λc I857
Sam7 DNA用Eco T14 I酶切反应后配制而成的。
本制品浓度为50
ng/μl，内含1×Loading Buffer，可根据实验需要，每次取5 μl～
20 μl电泳，使用方便，电泳图像清晰。
●保存温度：-20℃
●使用注意： 1． λDNA digest DNA Markers的原始末端之间经常由COS末
端结合在一起，在电泳前进行热处理（60℃, 5分钟），能使
Marker的电泳图像变得更为清晰。
以λ-Eco T14 I digest DNA Marker为例，原始末端之间的
COS末端的结合，影响19,329 bp和4,254 bp的DNA条带。
如果电泳以后的图像中的4,254 bp的DNA条带亮度低于比其
小的DNA片段时，那说明COS末端的结合情况比较严重。
2．电泳时的加样孔宽度小于6 mm时，每次取5μl制品电泳便可
得到清晰条带。
如果加样孔增宽，须适当增加Marker制品的加
样量。
3． 对DNA电泳而言，Agarose的纯度对DNA条带的清晰度影响
很大。
因此，电泳时应尽量选用质量好的Agarose。
Agarose电泳图像示意图
V2010.04。
TaKaRa 蛋白质分子量标准(低)
∙o Protocolso Standard Protocolo Manual/Datasheet∙Cat.# Product Size Note3450 Protein Molecular Weight Marker (Low) 200 lanes3451 Protein Molecular Weight Marker (High) 200 lanes3452 Protein Molecular Weight Marker (Broad) 200 lanesDescriptionProtein Molecular Weight Markers are designed for use in SDS-Polyacrylamide gel electrophoresis. There are 3 kinds of Protein Molecular Weight Marker, Low (Molecular weight range : 14.3 - 97.2 kDa) , High (Molecular weight range : 44.3 - 200 kDa) and Broad (Molecular weight range : 6.5 - 200 kDa).Each protein is proportioned to yield uniform band intensities when perform staining Coomassie Brilliant Blue R-250 after run on SDS polyacrylamide gel.5µl of 20-fold diluted marker is loaded per lane of SDS-PAGE minigel for standard use. In this case, this marker is sufficient for 200 lanes.Electrophoresis ResultLow(Cat.# 3450) High(Cat.# 3451) Broad(Cat.# 3452)15% SDS-PAGE 7.5% SDS-PAGE 5-20% gradientSDS-PAGEStorageProtein Molecular Weight Marker, 1 MDTT: -20°C5×Loading Buffer : Store at room temperature after used.ComponentProtein Molecular Weight Marker 50 µl5×Loading Buffer1 ml1 M DTT 100 µlConcentration12 µg/µl (Cat.# 3450) 10 µg/µl (Cat.# 3451) 18 µg/µl (Cat.# 3452)Form50 mM Tris-HCl, pH6.81 mM EDTA200mMNaCl50% glycerolComponent Protein Low(Cat.# 3450)Protein Source M.W.(Da)Phosphorylase B Rabbitmuscle97,200Serum Albumin Bovine 66,409Ovalbumin Hen eggwhite44,287Carbonic Bovine 29,000anhydraseTrypsin Inhibitor Soybean 20,100Lysozyme Hen eggwhite14,300High(Cat.# 3451)Protein Source M.W.(Da)Myosin Pig 200,000 β-galactosidaseE. coli116,000Phosphorylase B Rabbitmuscle97,200SerumAlbuminBovine 66,409Ovalbumin Hen eggwhite44,287Broad(Cat.# 3452) < /TR>Protein Source M.W.(Da)Myosin Pig 200,000 β-galactosidase E. coli116,000 Phosphorylase B Rabbit muscle 97,200 Serum Albumin Bovine 66,409Ovalbumin Hen eggwhite44,287CarbonicanhydraseBovine 29,000 Trypsin Inhibitor Soybean 20,100Lysozyme Hen eggwhite14,300Aprotinin Bovinepancreas6,5005×Loading Buffer200 mM Tris-HCl , pH6.810% SDS 0.05%BPB50% GlycerolNote∙All products are intended to be used for research purpose only. They are not to be used for drug or diagnostic purposes, nor are they intended for human use. They shall not to be used products as food, cosmetics, or utensils, etc∙Takara products may not be resold or transferred, modified for resale or transfer, or used to manufacture commercial products without written approval from TAKARA BIO INC.∙If you require licenses for other use, please call at +81 77 543 7247 or contact from our website at ∙Please confirm the content about the license or patent used in this document that relates to the Takara Bio product by clicking the license mark.Moreover, please confirm the "Limited Use Label License" or "patent" concerning the product of another manufacturers or respective owners in their Web site/catalog etc.Page TOP。
Takara说明书
Code No. RR047A 研究用PrimeScript TM RT reagent Kitwith gDNA Eraser(Perfect Real Time)说明书目录内容页码●制品说明1●制品内容 1 ●试剂盒外必备材料 1 ●保存 1 ●特长 2 ●使用注意 2 ●操作方法 2 ●Real Time PCR 4 ●实验例 6 ●附录7 ●关联产品8●制品说明为了准确地进行基因表达量分析，必须满足只有cDNA作为模板检出的先决条件，但Total RNA中常常混有基因组DNA，并可以直接作为PCR反应的模板进行扩增，因此会造成解析结果不准确。
为了避免这种情况发生，通常将检测用引物设计在内含子前后的外显子上，使基因组DNA得不到扩增。
但是，此方法不适合具有单个外显子的基因或两个外显子之间所跨的内含子过小的基因，同时当基因组上有伪基因存在时、或设计引物对基因组有非特异性扩增时、以及基因信息没被完全解析的生物种等也同样不适合于本方法。
在这种情况下，我们常常需要对Total RNA样品进行DNase I处理，以除去残存的基因组DNA。
而DNase I处理通常要进行复杂的纯化操作，同时会造成RNA的降解和损失。
PrimeScript RT reagent Kit with gDNA Eraser是可以除去基因组DNA进行Real Time RT-PCR反应的专用反转录试剂。
Kit中使用了具有较强DNA分解活性的gDNA Eraser，通过42℃，2 min即可除去基因组DNA。
同时由于反转录试剂中含有抑制DNA分解酶活性的组分，经过gDNA Eraser处理后的样品可以直接进行15 min的反转录反应合成cDNA，因此，20 min内即可迅速完成从基因组DNA去除到cDNA 合成的全过程。
使用本制品合成的cDNA适用于SYBR® Green分析法和TaqMan®探针分析法，可以根据实验目的，选择与SYBR®Premix Ex Taq II（Tli RNaseH Plus）、Premix Ex Taq（Probe qPCR）等定量试剂组合使用。
Takara基因组抽提试剂盒说明书
3. 准备 65℃水浴。 4. Solution A、Solution B 若出现沉淀，请于 65℃加热溶解，待恢复至室温后使用。 5. Rinse B 在首次使用前，请添加 56 ml 的 100%乙醇。 6. 洗脱结合于 DNA 制备膜上的基因组 DNA 时，把 Elution Buffer 或灭菌蒸馏水加热至 65℃使用将
●制品内容（50 次量）
本试剂盒分试剂 Set 与 Column Set 两部分。 ■ 试剂 Set
RNase A1（50 mg/ml）*1
20 μl
Lysozyme
60 mg
Glycerol
1.2 ml
EDTA Buffer
2 ml
SP Buffer
10 ml
Solution A*2
12 ml
Solution B*2
注）注意不要残留细小菌块，可以使用振荡器（Vortex） 等剧烈振荡使菌体充分悬浮。
4. 加入 20 μl 的 Lysozyme 溶液，均匀混合后室温静置 5 分钟。
5. 加入 30 μl 的 EDTA Buffer，均匀混合后室温静置 5 分钟。
6. 加入 200 μl 的 Solution A，剧烈振荡后 65℃保温 10
后应充分混合。 ② Lysozyme 失活。Lysozyme 配制成溶液后应保证在-20℃保存。 ③ 洗脱时将灭菌蒸馏水或 Elution Buffer 加热至 65℃后使用将有利于提高洗脱效率。 ④ 严格按照操作方法进行操作。