130 00 1_B00980155-01_Cell magazine
promega celltiter-fluor cell viability assay protocol
Promega Corporation ·2800 Woods Hollow Road ·Madison, W I 53711-5399 USA Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·1.Description ..........................................................................................................12.Product Components and Storage Conditions ............................................43.Reagent Preparation and Storage ...................................................................54.Protocols for the CellTiter-Fluor™ Cell Viability Assay ..........................5A.Determining Assay Sensitivity, Method 1........................................................6B.Determining Assay Sensitivity, Method 2........................................................7C.Example Viability Assay Protocol.....................................................................8D.Example Multiplex Assay Protocol (with luminescent caspase assay).......9E.Recommended Controls (10)5.General Considerations ..................................................................................106.References .........................................................................................................117.Related Products ..............................................................................................121.DescriptionThe CellTiter-Fluor™ Cell Viability Assay (a)is a nonlytic, single-reagent-addition fluorescence assay that measures the relative number of live cells in a culture population after experimental manipulation (Figures 1 and 2). The CellTiter-Fluor™ Cell Viability Assay measures a conserved and constitutive protease activity within live cells and therefore serves as a marker of cell viability (1). Results obtained using the CellTiter-Fluor™ Cell Viability Assay correlate well with other established methods of determining cell viability(Figure 3). The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant, peptide substrate (glycyl-phenylalanyl-aminofluorocoumarin; GF-AFC). The substrate enters intact cells where it is cleaved by the live-cell protease activity to generate a fluorescent signal proportional to the number of living cells (Figure 4). This live-cell protease becomes inactive upon loss of cell membrane integrity and leakage into the surrounding culture medium.The CellTiter-Fluor™ Cell Viability Assay also can be used in a single-well,sequential, multiplex format with other downstream chemistries to normalize data by cell number. Data from the assay can serve as an internal control andCellTiter-Fluor™ Cell Viability AssayAll technical literature is available on the Internet at: /protocols/ Please visit the web site to verify that you are using the most current version of this Technical Bulletin. Please contact Promega Technical Services if you have questions on useof this system. E-mail: techserv@allow identification of errors resulting from cell clumping or compoundcytotoxicity. The CellTiter-Fluor™ Cell Viability Assay is compatible with most Promega luminescence assays or spectrally distinct fluorescence assay methods,such as assays measuring caspase activation, reporter gene expression or orthogonal measures of viability. However, some P450-Glo™ multiplexprotocols may require removing culture supernatant to a separate assay well before performing the assay because of isoform-specific competitive inhibition of the cytotochrome P450 enzymes by the coumarin product of the CellTiter-Fluor™ Cell Viability Assay reaction.Figure 1. Schematic diagram of the CellTiter-Fluor™ Cell Viability Assay.Promega Corporation ·2800 Woods Hollow Road ·Madison, W I 53711-5399 USA Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·6868M ACellTiter-Fluor™ ReagentAdd GF-AFC Substrate to Assay Buffer to create the CellTiter-Fluor™ Reagent.MeasureAssay BenefitsMeasure the Relative Number of Live Cells in Culture: Nonlytic, single-reagent-addition, homogeneous, “add-mix-measure” protocol.Get More Data from Every Well: The CellTiter-Fluor™ Cell Viability Assay can be performed in multiplex with most Promega luminescence assays.Normalize Data for Cell Number: Normalizing data for live-cell number makes results more comparable well-to-well, plate-to-plate, day-to-day.Figure 3. The CellTiter-Fluor™ Cell Viability Assay shows strong correlation with established methods for measuring viability. Panel A. The GF-AFC Substrate signal from serial dilutions of live cells plotted against results from the CellTiter-Glo ®Luminescent Cell Viability Assay (Cat.# G7570), which measures cellular ATP.Panel B.The GF-AFC Substrate signal from serial dilutions of live cells plottedagainst results achieved using the CellTiter-Blue ®Cell Viability Assay (Cat.# G8080).Promega Corporation ·2800 Woods Hollow Road ·Madison, W I 53711-5399 USA Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·6867M AA.B.R e s o r u f i n F l u o r e s c e n c e (R F U )2,5002,6002,7002,8002,9003,0003,100GF-AFC Fluorescence (RFU)A T P -B a s e d A s s a y L u m i n e s c e n c e (R L U )0GF-AFC Fluorescence (RFU)LIVE-CELL SUBSTRATE:cell-permeant fluorogenic substrate for the live-cellprotease (Gly-Phe-AFCoumarin)NucleusLive CellLive-cell protease substrate can cross the cell membrane.Active Live-CellProteaseO CF 3GF–N HOGFOCF 3OH 2N6995M AFigure 2. CellTiter-Fluor™ Cell Viability Assay chemistry. The cell-permeant substrate enters the cell, where it is cleaved by the live-cell protease activity to produce the fluorescent AFC. The live-cell protease is labile in membrane-compromised cells and cannot cleave the substrate.Figure 4. The CellTiter-Fluor™ Cell Viability Assay signal derived from viable cells (untreated) is proportional to cell number. Dead cells (treated) do not contribute appreciable signal in the assay.2.Product Components and Storage ConditionsProductSize Cat.#CellTiter-Fluor™ Cell Viability Assay10mlG6080Cat.# G6080 contains sufficient reagents for 100 assays at 100µl/assay in a 96-well plate format or 400 assays at 25µl/assay in a 384-well plate format. Includes:• 1 × 10ml Assay Buffer• 1 × 10µl GF-AFC Substrate (100mM in DMSO)ProductSize Cat.#CellTiter-Fluor™ Cell Viability Assay5 × 10mlG6081Cat.# G6081 contains sufficient reagents for 500 assays at 100µl/assay in a 96-well plate format or 2,000 assays at 25µl/well in a 384-well format. Includes:• 5 × 10ml Assay Buffer• 5 × 10µl GF-AFC Substrate (100mM in DMSO)ProductSize Cat.#CellTiter-Fluor™ Viability Assay2 × 50mlG6082Cat.# G6082 contains sufficient reagents for 1,000 assays at 100µl/assay in a 96-well plate format or 4,000 assays at 25µl/well in a 384-well format. Includes:• 2 × 50ml Assay Buffer• 2 × 50µl GF-AFC Substrate (100mM in DMSO)Storage Conditions: Store the CellTiter-Fluor™ Cell Viability Assaycomponents at –20°C. See product label for expiration date.Promega Corporation ·2800 Woods Hollow Road ·Madison, W I 53711-5399 USA Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·6866M ACells or Cell Equivalents/WellA F C F l u o r e s c e n c e (R F U )3.Reagent Preparation and Storagepletely thaw the CellTiter-Fluor™ Cell Viability Assay components in a37°C water bath. Vortex the GF-AFC substrate to ensure homogeneity, thenbriefly centrifuge for complete substrate volume recovery.2.Transfer the GF-AFC Substrate (10µl for Cat.# G6080 and G6081; 50µl for Cat.#G6082) into the Assay Buffer container (10ml for Cat.# G6080 and G6081; 50mlfor Cat.# G6082) to form a 2X Reagent. Mix by vortexing the contents until thesubstrate is thoroughly dissolved.Note: The solution may initially appear “milky” when the GF-AFC substrate isdelivered to the buffer. This is normal. The substrate will dissolve withvortexing. The CellTiter-Fluor™ Reagent may be scaled to accommodate thevolumes required for downstream multiplexes. To do this, use 1/5 the volumeof buffer when you prepare the reagent (i.e., 10µl of the GF-AFC Substrate in2ml of Assay Buffer). Be sure to label the bottle to indicate that this is a moreconcentrated reagent, suitable for multiplex assays. Add the reagent at 1/5 thevolume of the cell culture.Storage: The CellTiter-Fluor™ Viability Reagent should be used within 24hours if stored at room temperature. Unused GF-AFC Substrate and AssayBuffer can be stored at 4°C for up to 7 days with no appreciable loss ofactivity.4.Protocols for the CellTiter-Fluor™ Cell Viability AssayMaterials to Be Supplied by the User•96-, 384-, or 1536-well opaque-walled tissue culture plates compatible with fluorometer (clear or solid bottom)•multichannel pipettor or liquid-dispensing robot•reagent reservoirs•fluorescence plate reader with filter sets for AFC (380–400nm Ex/505Em)•orbital plate shaker•compound known to cause 100% cytotoxicity or lytic detergent (digitonin, Calbiochem Cat.# 300410 or Sigma-Aldrich Cat.# D141 at 20mg/ml in DMSO).If you have not performed this assay on your cell line previously, werecommend determining assay sensitivity using your cells and one of the two methods described below (Section 4.A or 4.B). If you do not need to determineassay sensitivity for your cells, proceed to Section 4.C.Promega Corporation·2800 Woods Hollow Road ·Madison, W I 53711-5399 USA Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·6.Dilute digitonin to 300µg/ml in water. Using a multichannel pipet,carefully add 10µl of the diluted digitonin to all wells of columns 7–12 to lyse cells (treated samples). Add 10µl of water to all wells of columns 1–6to normalize the volume (untreated cells).7.Add 100µl of the CellTiter-Fluor™ Reagent to all wells, mix briefly by orbital shaking and incubate at 37°C for at least 30 minutes.Note:Longer incubations may improve assay sensitivity and dynamic range. However, do not incubate longer than 3 hours, and be sure to shieldplates from ambient light.Promega Corporation ·2800 Woods Hollow Road ·Madison, W I 53711-5399 USA Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·8.Measure resulting fluorescence with a fluorometer (380–400nm Ex /505nm Em )Note: You may need to adjust instrument gains (applied photomultiplier tube energy).9.Calculate the practical sensitivity for your cell type by making a signal-to-noise calculation for each dilution of cells (10,000 cells/well; 5,000cells/well; 2,500 cells/well, etc.).Viability S:N =(Average Untreated – Average Treated)Note: The practical level of assay sensitivity for the assay is a signal-to-noise ratio of greater than 3 standard deviations (derived from reference 1).4.B.Determining Assay Sensitivity, Method 21.Harvest adherent cells (by trypsinization, etc.), wash with fresh medium (to remove residual trypsin) and resuspend in fresh medium.Note:For cells growing in suspension, proceed to Step2.2.Determine the number of viable cells by trypan blue exclusion using a hemacytometer, then adjust the cells by dilution to 100,000 viable cells/ml in at least 20ml of fresh medium.Note:Concentrate the cells by centrifuging and removing medium if the pool of cells is less than 100,000 cells/ml.3.Divide the volume of diluted cells into separate tubes. Subject one tube to "moderate" sonication (empirically determined by postsonicationmorphological examination) to rupture cell membrane integrity and to simulate a 100% dead population. The second tube of untreated cells will serve as the maximum viable population.4.Create a spectrum of viability by blending sonicated and untreatedpopulations in 1.5ml microcentrifuge tubes as described in Table 2.Promega Corporation ·2800 Woods Hollow Road ·Madison, W I 53711-5399 USA Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·4.B.Determining Assay Sensitivity, Method 2 (continued)5.After mixing each blend by gently vortexing, pipet 100µl of each blend into8 replicate wells of a 96-well plate. Add the 100% viable cells to column 1,95% viable to column 2, etc. Add cell culture medium only to column 10 toserve as a no-cell control.6.Add CellTiter-Fluor™ Reagent in an equal volume (100µl per well) to allwells, mix briefly by orbital shaking, then incubate for at least 30 minutesat 37°C.Note:Longer incubations may improve assay sensitivity and dynamicrange. However, do not incubate longer than 3 hours, and be sure to shieldplates from ambient light.7.Measure resulting fluorescence with a fluorometer (380–400nm Ex/505nm Em).Note: You may need to adjust instrument gains (applied photomultipliertube energy).8.Calculate the practical sensitivity for your cell type by making a signal-to-noise calculation for each blend of cell viability (X = 95, 90%, etc.).Viability S:N = (Average 100% – Average X%)Standard Deviation of 0% (viable cells)Note: The practical level of assay sensitivity for the assay is a signal-to-noise ratio of greater than 3 standard deviations (derived from reference 1).4.C.Example Viability Assay Protocol1.Set up 96-well assay plates containing cells in culture medium at desireddensity.2.Add test compounds and vehicle controls to appropriate wells so that thefinal volume is 100µl in each well (25µl for a 384-well plate).3.Culture cells for the desired test exposure period.4.Add CellTiter-Fluor™ Reagent in an equal volume (100µl per well) to allwells, mix briefly by orbital shaking, then incubate for at least 30 minutesat 37°C.Note:Longer incubations may improve assay sensitivity and dynamicrange. However, do not incubate more than 3 hours, and be sure to shieldplates from ambient light.5.Measure resulting fluorescence using a fluorometer (380–400nm Ex/505nm Em).Note:You may need to adjust instrument gains (applied photomultipliertube energy).Promega Corporation·2800 Woods Hollow Road ·Madison, W I 53711-5399 USA Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·4.D.Example Multiplex Assay Protocol (with luminescent caspase assay)1.Set up 96-well assay plates containing cells in culture medium at the desired density.2.Add test compounds and vehicle controls to appropriate wells so that the final volume is 100µl in each well (25µl for a 384-well plate).3.Culture cells for the desired test exposure period.Note:Caspase activation is a transient event dictated by compound potency and cell cycle susceptibility. Time course experiments are often useful for defining peak caspase activity and cytotoxicity.4.Add 20µl of CellTiter-Fluor™ Reagent (prepared as 10µl substrate in 2ml Assay Buffer) to all wells, and mix briefly by orbital shaking. Incubate for at least 30 minutes at 37°C.Note: Longer incubations may improve assay sensitivity and dynamic range. However, do not incubate longer than 3 hours, and be sure to shield plates from ambient light.5.Measure resulting fluorescence using a fluorometer (380–400nm Ex /505nm Em ).Note: You may need to adjust instrument gains (applied photomultiplier tube energy).6.Add an equal volume of Caspase-Glo ®3/7 Reagent prepared as described in Technical Bulletin #TB323 to wells (100–120µl per well), incubate for 30minutes, then measure luminescence using a luminometer.Figure 5. Multiplex of CellTiter-Fluor™ Assay and Caspase-Glo ®3/7 Assay.The CellTiter-Fluor™ Reagent was added to wells and viability measured after incubation for 30 minutes at 37°C. Caspase-Glo ®3/7 Reagent was added andluminescence measured after a 30-minute incubation (10,000 cells/well).Promega Corporation ·2800 Woods Hollow Road ·Madison, W I 53711-5399 USA Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·6865M A® 3/7 Assaylog 10[paclitaxel] ML u m i n e s c e n c e (R L U )F l u o r e s c e n c e (R F U )4.E.Recommended ControlsNo-Cell Control: Set up triplicate wells without cells to serve as a control to determine background fluorescence.Untreated Cells Control:Set up triplicate wells with untreated cells to serve as a vehicle control. Add the same solvent used to deliver the test compounds to the vehicle control wells.Optional Test Compound Control: Set up triplicate wells without cells but containing the vehicle and test compound to test for possible interference with the assay chemistry.Positive Control for Viability:Set up triplicate wells containing cells treated with a compound known to be toxic to the cells used in your model system.5.General ConsiderationsOptical Filters and Instrumentation:Fluorogenic dyes exhibit distinct absorption (excitation) and emission profiles when a light energy source is applied. Most fluorometers or multimode instruments contain optical band-pass filters that restrict the wavelengths of light used to excite a fluorophore and the wavelengths passing through to the detector. Note that deviation from the optimal filter set recommendations (Figure 6) may adversely affect assay sensitivity and performance.Figure 6. Optimal excitation and emission spectra for AFC.Background Fluorescence and Inherent Serum Activity:Tissue culturemedium that is supplemented with animal serum may contain detectable levels of the protease marker used to measure live-cells. This protease activity may vary among different lots of serum. To correct for variability, determine background fluorescence using samples containing medium plus serum without cells.Temperature: The generation of fluorescent product is proportional to the live-cell protease activity. The activity of this protease is influenced by temperature.Promega Corporation ·2800 Woods Hollow Road ·Madison, W I 53711-5399 USA Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·6864M AWavelength (nm)F l u o r e s c e n c e (R F U )For best results, we recommend incubating at a constant controlled temperatureto ensure uniformity across the plate. After adding reagent and briefly mixing,we suggest one of two options:1.At 37°C in a water-jacketed incubation module (Me’Cour, etc.).Note:Incubation at 37°C in a CO2culture cabinet may lead to edge-effectsresulting from thermal gradients.2.At room temperature with or without orbital shaking.Note: Assays performed at room temperature may require more than30minutes of incubation for optimal sensitivity. However, do not incubatelonger than 3 hours.Assay Controls: In addition to a no-cell control to establish background fluorescence, we recommend including a maximum viability (untreated cells)and maximum cytotoxicity control in the experimental design. The maximum viability control is established by adding vehicle only (used to deliver the test compound to test wells). In most cases, this consists of a buffer system ormedium and the equivalent amount of solvent added with the test compound.The maximum cytotoxicity control can be determined using a compound thatcauses 100% cytotoxicity or a lytic compound added to compromise viability (digitonin). See Section 4.A.Viability Marker Half-Life:The activity of the protease marker found has nohalf-life in viable cells. Viable cells will process the substrate to liberate the AFC fluorophore. However, when cells lose membrane integrity, the protease activity declines very quickly. Therefore enzymatic instability of the live-cell proteaseoutside of viable cells establishes GF-AFC as a good marker for cell viability.Light Sensitivity: Although the GF-AFC Substrate demonstrates good general photostability, the liberated AFC fluorophore (after contact with protease) can degrade with prolonged exposure to ambient light sources. We recommend shielding the plates from ambient light at all times.Cell Culture Medium:The GF-AFC Substrate is introduced into the test wellusing an optimized buffer system that mitigates differences in pH fromtreatment. In addition, the buffer system supports protease activity in a host of different culture media with varying osmolarity. With the exception of media formulations with either very high serum content or phenol red indicator, no substantial performance differences will be observed among media.6.References1.Niles, A.L. et al. (2007) A homogeneous assay to measure live and dead cells in thesame sample by detecting different protease markers. Anal. Biochem.366, 197–206.2.Zhang, J-H. et al.(1999) A simple statistical parameter for use in evaluation andvalidation of high-throughput screening assays. J. Biomol. Screen. 4, 67–73.Promega Corporation·2800 Woods Hollow Road ·Madison, W I 53711-5399 USA Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·7.Related ProductsCell Viability and CytotoxicityAssays ProductSize Cat.#MultiTox-Fluor Multiplex Cytotoxicity Assay 10ml G9200MultiTox-Glo Multiplex Cytotoxicity Assay 10ml G9270CytoTox-Glo™ Cytotoxicity Assay 10ml G9290CytoTox-Fluor™ Cytotoxicity Assay10ml G9260CellTiter-Glo ®Luminescent Cell Viability Assay 10mlG7570CytoTox-ONE™ Homogeneous Membrane Integrity Assay1,000–4,000 assaysG7891CellTiter-Blue ®Cell Viability Assay20mlG8080Additional Sizes Available.Apoptosis AssaysProductSize Cat.#Caspase-Glo ®2 Assay 10ml G0940Caspase-Glo ®6 Assay 10ml G0970Caspase-Glo ®3/7 Assay 10ml G8091Caspase-Glo ®8 Assay 10ml G8201Caspase-Glo ®9 Assay10ml G8211Apo-ONE ®Homogeneous Caspase 3/7 Assay10ml G7790Additional Sizes Available.Reporter Gene AssaysProductSize Cat.#Bright-Glo™ Luciferase Assay System 10ml E2610Steady-Glo ®Luciferase Assay System10ml E2510Additional Sizes Available.Promega Corporation ·2800 Woods Hollow Road ·Madison, W I 53711-5399 USA Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·(a)Patent Pending.© 2007–2012 Promega Corporation. All Rights Reserved.Apo-ONE, Caspase-Glo, CellTiter-Blue, CellTiter-Glo and Steady Glo are registered trademarks of Promega Corporation.BrightGlo, CellTiter-Fluor, CytoTox-Fluor, CytoTox-Glo, CytoTox-ONE and P450-Glo and are trademarks of Promega Corporation.Products may be covered by pending or issued patents or may have certain limitations. Please visit our Web site for more information.All prices and specifications are subject to change without prior notice.Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the most up-to-date information on Promega products.。
VersaFlow 徐特里 Therrolis 100 零形系列用料选择指南 CM04 50 不铬钢
36-CM-16U-28Issue 11Page 1 of 2VersaFlow Coriolis 100Model Selection GuideCM04 Size 50 Stainless SteelSmartLine®36-CM-16U-28 Issue 11 Page 2 of 2Secondary Containment Information + Polishing InformationNote 1Secondary Containment InformationThe following information is provided to try to simplify the selection of the secondary containment /outer casingoptionG All externals SS 304/L No secondary pressure containment. Typical burst pressure > 100 barH All externals SS 316/L No secondary pressure containment. Typical burst pressure > 100 bar0All externals SS 304/L Max Sec. Pressure containment 63 bar/913 psi (PED approved)A All externals SS 316/L Max Sec. Pressure containment 63 bar/913 psi (PED approved)B All externals SS 316/L Max Sec. Pressure containment 100 bar/1450 psi (PED approved)Notes:1. There are no longer any flange constraints for options G and H2. You may now choose the required outer casing (option G and H) in combination with any process connection irrespective of the pressure rating.3. Most applications do not require secondary containment, so the 304L (option G) may be used unless 316L is specifically requested.4. The food and pharmaceutical industries require 316L materials in most cases so option H will be suitable here.5. Options 0, A and B are available for customers who still require PED approved secondary containment.6. On Options 0, A and B flanges with higher pressure ratings than the secondary pressure containment can not be ordered.WarningIn the case of high pressure gases, gases kept as liquids at high pressures and/or where there is a danger of the measuring tube failingdue to process conditions, e.g. with erosive or corrosive products, it is strongly recommended that a secondary pressure containment optionis purchased. Where process pressures exceed the secondary containment pressure rating, an optional burst disc should be fitted.This is highly recommended for High pressure gases. Please consult factory.Note 2Polishing Information1. To guarantee the surface finish of an CM Coriolis Meter, it is mandatory to order the polishing option as per the price list2. This is also mandatory for a meter requested with hygienic approvals3. For all other meters, the surface finish can not be guaranteed unless polishing is ordered as per 1.。
Nodinitib-1_DataSheet_MedChemExpress
Inhibitors, Agonists, Screening Libraries Data SheetBIOLOGICAL ACTIVITY:Nodinitib–1 (ML130;CID–1088438) is a NOD1 inhibitor with an IC 50 of 0.56 μM.IC50 & Target: IC50: 0.56 μM (NOD1)[1]In Vitro: Nodinitib–1 selectively inhibits IL–8 production induced by NOD1 ligand. Nodinitib–1 also inhibits γ–tri–DAP–induced expression of the prototypical NF–κB target gene IκBα at the mRNA level. Nodinitib–1 inhibits γ–tri–DAP–dependent activation of NF–κB (IκBα phosphorylation and degradation) and MAPK (p38 phosphorylation) signalings, without affecting Akt survival pathway.Nodinitib–1 selectively inhibits responses of primary dendritic cells to NOD1 ligand. Nodinitib–1 reduces cell surface expression of co–stimulatory molecules CD83, CD86 and HLA–DR and also inhibits expression of IL–1β, IL–6 and TNFα elicited by γ–tri–DAP (but not by LPS), without causing cytoxicity [1]. Nodinitib–1 is identified as NOD1–selective molecules from an HTS campaign involving ~290,000 compounds. Nodinitib–1 inhibits γ–tri–DAP–stimulated luciferase production in HEK 293T cells, which has endogenous NOD1 levels at submicromolar concentration as determined in a NF–κB–linked reporter assay [2].PROTOCOL (Extracted from published papers and Only for reference)Cell Assay:[1]RAW264.7 cells are treated with 5 μM of CID–1088438 or CID–44229067 alone, or also infected with S. typhimurium at multiplicity of infection (MOI) of 20 and 200 bacteria per mammalian cell. Cell viability is analyzed two hours after Salmonella infection by measuring ATP levels. Percentage viability is calculated according to the ATP levels of respective non–infected cells.Values presented are averages of two replicates (+ SEM)[1].References:[1]. Correa RG, et al. Discovery and characterization of 2–aminobenzimidazole derivatives as selective NOD1 inhibitors. Chem Biol. 2011 Jul 29;18(7):825–32.[2]. Hershberger PM, et al. Synthesis and physicochemical characterization of novel phenotypic probes targeting the nuclear factor–kappa B signaling pathway. Beilstein J Org Chem. 2013 May 8;9:900–7.Product Name:Nodinitib–1Cat. No.:HY-18639CAS No.:799264-47-4Molecular Formula:C 14H 13N 3O 2S Molecular Weight:287.34Target:NOD–like Receptor (NLR)Pathway:Immunology/Inflammation Solubility:DMSO: 20 mg/mLCaution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@ Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。
VM130中文资料
Pot en t i a l-Tr en n ver s tär k erVM130- VM138Di e Tr en n ver s tär k er r ei he VM130-VM138 a r bei t et n a ch dem Pr i n zi p der t r a n s f or m a t or i s chen Pot en t i a l t r en n u n g. Du r ch di e Bes tück u n g m i t m oder n s t en Ba u el em en t en w i r d ei n e s ehr g u t e Tem per a t u r- u n d Nu l l pu n k t k on s t a n z, ei n g er i n g er L i n ea r i tät s f ehl er u n d ei n e hohe Zu ver läs s i g-k ei t er r ei cht.Di e G erät e a r bei t en bi pol a r u n d w ei s en ei n e Dr ei w eg e-Tr en n u n g der P ot en t i a l e zw i s chen Ei n g a n g, A u s g a n g u n d Ver s or g u n g a u f.Di e Ba u r ei he u m f a s s t el f Typen. Si e er mög l i chen es Nor m s i g n a l e g a l va n i s ch g et r en n t zuüber t r a g en bzw. u m zu s et zen.Typenüber s i cht:Pot en t i a l Sepa r a t i on A m pl i f i er sVM130- VM138The s epa r a t i on a m pl i f i er s er i es VM130-VM138 oper a t es a ccor di n g t o t he pr i n ci pl e of t r a n s f or m a t or i c pot en t i a l s epa r a t i on. U s i n g m os t m oder n el em en t s r es u l t s i n hi g h t em per a t u r e a n d zer o con s t a n cy, l ow l i n ea r i t y er r or, a n d hi g h r el i a bi l i t y.The devi ces w or k bi pol a r l y a n d ha ve t hr ee-w a y s epa r a-t i on of t he pot en t i a l s bet w een i n pu t, ou t pu t, a n d s u ppl y. The s er i es com pr i s es el even t ypes a l l ow i n g el ect r i ca l i n s u l a t i on t r a n s m i s s i on r es pect i vel y con ver s i on of s t a n-da r d s i g n a l s.Type s u m m a r y:*Der G erät et yp VM135 ei g n et s i ch a u ch zu rÜber t r a g u n g von4...20m A⇒ 4 ...20m A!Zw ei Spi n del t r i m m er bi et en di e Mög l i chk ei t von der G erä-t ef r on t a u s di e Ver s tär k u n g u n d den N u l l pu n k t zu verän-der n.Di e G erät e s i n d m on t a g ef r eu n dl i ch a u f Tr a g s chi en en TS35 a u f zu r a s t en. * The t ype VM135 i s a l s o s u i t a bl e f or t r a n s m i s s i on of 4...20m A⇒ 4...20m A!Tw o hel i ca l t r i m m er s of f er t he pos s i bi l i t y t o a dju s t on t he f a ce of t he hou s i n g t he a m pl i f i ca t i on a n d t he zer o poi n t. The devi ces ca n be ea s i l y s n a p-m ou n t ed on t o m ou n t i n g r a i l s TS35.Typ Ei n g a n g A u s g a n g Hi l f s en er g i e Type I n pu t Ou t pu t A u x i l i a r y en er g y VM1300... ±10V 0... ±10V 24 VdcVM131 0... ±20m A0... ±10V 24 VdcVM132 4 ... 20m A0... 10V 24 VdcVM133 0... ±10V 0... ±20m A 24 VdcVM134 0... 10V 4 ... 20m A 24 VdcVM1350... ±20m A0... ±20m A 24 VdcVM136 0... 20m A 4 ... 20m A 24 VdcVM137 4 ... 20m A0... 20m A 24 VdcVM138/1 0... ±60m V 0... ±10V 24 VdcVM138/2 0... ±60m V 0... ±20m A 24 VdcVM138/3 0... 60m V 4 ... 20m A 24 VdcTechn i s che Da t en VM 130- VM 138Ver s or g u n g s s pa n n u n g: 24Vdc ±20% / Ri ppl e = 5% Lei s t u n g s a u f n a hm e: 1WSpa n n u n g s ei n g a n g (10V): 0...10V / R I N =100k Ω Spa n n u n g s ei n g a n g (60m V) : 0...60m V / R I N =100k Ω Über l a s t ba r k ei t (U-Ei n g a n g ) : m a x . 50V St r om ei n g a n g : 0(4)...20m A / Bür de=100Ω Über l a s t ba r k ei t (I -Ei n g a n g ) : m a x . 50m A Spa n n u n g s a u s g a n g / La s t : 0...10V / m a x . 20m A St r om a u s g a n g / Bür de : 0(4)...20m A / m a x . 500Ω Nu l l pu n k t ei n s t el l u n g : ±20% Ver s t är k u n g s ei n s t el l u n g: 0,5...1,5m a x . I s ol a t i on s s pa n n u n g: 1k V Über t r a g u n g s f r equ en z : 2k Hz (VM 138 = 35Hz) Li n ea r i t ät s f ehl er : 0,05% Tem per a t u r dr i f t: 0,0025%/KUm g ebu n g s t em per a t u r : 0...50°C A n s chl üs s e : Schr a u bk l em m en 2,5m m ² G ehäu s em a t er i a l : I s ol i er s t of f g r a u G ehäu s ebef es t i g u n g : Schn a ppbef es t i g u n g f ür TS35 G ehäu s em a ße : s i ehe Zei chn u n g G ew i cht: 110gTechn i ca l Da t a VM 130- VM 138Su ppl y vol t a g e: 24Vdc ±20% / Ri ppl e ≤ 5% Pow er con s u m pt i on: 1WVol t a g e i n pu t (10V) : 0…10V / R I N=100k Ω Vol t a g e i n pu t (60m V) : 0...60m V / R I N =100k Ω Over l oa d ca pa ci t y (Vol t a g e i n pu t s ) : m a x . 50V Cu r r en t i n pu t : 0(4)...20m A / Bu r den =100Ω Over l oa d ca pa ci t y (Cu r r en t i n pu t ) : m a x . 50m A Vol t a g e ou t pu t / Loa d : 0...10V / m a x . 20m A Cu r r en t ou t pu t / Bu r den : 0(4)...20m A / m a x . 500Ω Zer o poi n t a dju s t m en t : ±20% A m pl i f i ca t i on a dju s t m en t : 0.5...1.5m a x . i n s u l a t i on vol t a g e: 1 k V Tr a n s m i s s i on f r equ en cy : 2k Hz (VM 138 = 35Hz) Li n ea r i t y er r or : 0.05% Tem per a t u r e dr i f t: 0.0025%/KA m bi en t t em per a t u r e: 0...50°C Con n ect i on s : Scr ew -t ype t er m i n a l s 2.5 m m ² Hou s i n g m a t er i a l : I n s u l a t i n g m a t er i a l g r ey Fa s t en i n g of hou s i n g : Sn a p-on f or TS35 Di m en s i on s of hou s i n g : cf . dr a w i n g W ei g ht: 110g。
2010年 材料类SCI 期刊的影响因子
2010年材料类SCI 期刊的影响因子Nature 自然 34.48Science 科学 29.747Nature Materials 自然（材料） 29.504Nature Nanotechnology 自然（纳米技术） 26.309Progress in materials science 材料科学进展 20.846Nature Physics 自然（物理） 16.891Progress in Polymer Science 聚合物科学进展 16.819Surface Science Reports 表面科学报告 12.808materials science & engineering r-reports 材料科学与工程报告12.619AngewandteChemie-International Edition 应用化学国际版 11.829 Nano Letters 纳米快报 10.991Advanced Materials 先进材料 8.379Journal of the American Chemical Society 美国化学会志 8.58 Annual Review of Materials Research 材料研究年度评论 7.947 Physical Review Letters 物理评论快报 7.328Advanced Functional Materials 先进功能材料 6.99MRS bulletin 材料研究学会（美国）公告 6.33Small 微观 6.171Chemical Communications 化学通信 5.504Progress in Surface Science 表面科学进展 5.429Chemistry of Materials 材料化学 5.368International Journal of Plasticity 4.791Journal of Materials Chemistry 材料化学杂志 4.795Carbon 碳 4.504Electrochemistry Communications 电化学通讯 4.243Inorganic Chemistry 无机化学 4.657The Journal of PhysicalL Chemistry B物理化学杂志，B辑：材料、表面、界面与生物物理 4.189Crystal Growth & Design 晶体生长与设计 4.162Physical Chemistry Chemical Physics 物理化学 4.116Langmuir 朗缪尔 4.097Solar Energy Materials and Solar Cells 太阳能材料，太阳能电池3.858Journal of power sources 电源技术 3.792Actamaterialia 材料学报 3.76Advances in Catalysis 催化进展 3.571Applied Physics Letters 应用物理快报 3.554Physical Review B 物理学评论 B 3.475The Journal of Physical Chemistry B 3.471International Materials Review 际材料评论 3.462Journal of the Mechanics and Physics of Solids 固体力学与固体物理学杂志 3.317Nanotechnology 纳米技术 3.137Journal of Applied Crystallography 应用结晶学 3.018 Microscipy and Microanalysis 微观与微观分析 2.992Current Opinion in Solid State & Materials Science 固态和材料科学的动态 2.976Scriptamaterialia 材料快报 2.949Composites Science and Technology 复合材料科学与技术 2.901 The Journal of Physical Chemistry A 物理化学杂志，A辑 2.899 Nanoscale Research Letters 纳米研究快报 2.894Biometals 生物金属 2.801Microporous and Mesoporous Materials 多孔和类孔材料 2.652 Science and Technology of Advanced Materials 先进材料科学与技术 2.599Journal of nanoparticle research 2.478Journal of Solid State Chemistry 2.34Current Nanoscience 当代纳米科学 2.437Solid State Ionics 固体离子 2.425IEEE Journal of Quantum Electronics IEEE量子电子学杂志 2.413 Mechanics of Materials 材料力学 2.374Corrosion Science 腐蚀科学 2.316Journal of Nanoparticle Research 纳米颗粒研究 2.299Journal of the Electrochemical Society 2.241Intermetallics 金属间化合物 2.231Journal of Applied Physics 应用物理杂志 2.201IEEE Transactions on Nanotechnology IEEE 纳米学报 2.154 Progress in Crystal Growth and Characterization of Materials 晶体生长和材料表征进展 2.129Journal of Physics D-Applied Physics 物理杂志D——应用物理2.104Journal of the American Ceramic Society 美国陶瓷学会杂志 2.101 Journal of Alloys and Compounds 合金和复合材料杂志 2.135 Journal of Chemica&Enginering Data 化学和工程资料杂志 2.063 Journal of the European ceramic society 欧洲陶瓷学会杂志 2.09 Materials Chemistry and Physics 材料化学和物理 2.015 Composite Structures 2.006Journal of Physics: Condensed Matter 物理学学报：凝聚态物质1.964Synthetic Metals 合成金属 1.962International Journal of Heat and Mass Transfer 1.947 Materials Letters 材料快报 1.94Journal of Nuclear Materials 1.933Materials science ＆ engineering A 1.901Materials Research Bulletin 1.879Materials Science ＆ Engineering C 1.842Solid State Communications 1.837Electrochemical and Solid State Letters 固体电化学快报 1.837 Diamond and Related Materials 金刚石及相关材料 1.822Journal of Solid State Electrochemistry 1.821International Journal of Solids and Structures 1.809Surface Science 表面科学 1.798Surface & coatings technology 1.793Wear 1.771International Journal of Thermal Sciences 1.77Advanced Engineering Materials 1.761Materials Science ＆ Engineering B 1.756Powder Technology 1.745ThermochimicaActa 1.742Thin Solid Films 1.727Materials Research Innovations 1.723Composites Part B – Engineering 1.704Journal of Applied Electrochemistry 1.697Journal of Chemical & Engineering Data 1.695Ceramics International 1.686IEEE Transactions on Nanotechnology 1.671Solid State Sciences 固体科学 1.675Journal of Materials research 1.667International Journal of Applied Ceramic Technology 1.627 Applied Surface Science 1.616Applied Physics A – Materials Science ＆ Processing 1.595 Journal of Thermal Analysis and Calorimetry 1.587Current Applied Physics 1.586Metallurgical and Materials Transactions A 1.564Materials & Design 1.518Journal of Materials Science 1.471European Physical Journal B -- Condensed Matter 1.466 Journal of Nanoscience and Nanotechnology 1.435Journal of Materials Processing Technology 1.42Materials Characterization 1.416Journal of Sol-gel Science and Technology 1.393Journal of Thermal Spray Technology 1.338Journal of Non-Crystalline Solids 1.252International Journal of Nanotechnology 1.234Journal of Intelligent Material Systems and Structures 1.177 Oxidation of Metals 1.108Physica B: Condensed Matter 1.056Surface and Interface Analysis 0.998Corrosion 0.952Metallurgical and Materials Transactions B 0.932 International Journal of materials research 0.862Journal of the Ceramic Society of Japan 0.862Journal of Materials Science & Technology 0.828J. enginee. mater. Technol.-transactions of the asme 0.815 Journal of Composite Materials 0.806Materials Science and Technology- London 0.794 International Journal of Thermophysics 0.702Materials Transactions 0.795Journal of Porous Materials 0.788Materials and Structures 0.753Journal of Thermophysics and Heat Transfer 0.687Advances in Applied Ceramics 0.642Journal of Energetic Materials 0.567Reviews on Advanced Materials Science 0.558Surface Engineering 0.432Journal of Phase Equilibria and Diffusion 0.415Journal of Ceramic Processing Research 0.402Surface Review and Letters 0.366Solid State Technology 0.275。
Unique PMO Plus Mixproof Sanitary Valve 零件清单说明书
TD 449-360 Reg.: 2009-01Intro.: 2009-012900-547PMO 2”PMO 2½”PMO 3”PMO 4”Pos.Qty.Denomination Seat ø81.3Seat ø115.3Seat ø143.9381O-ring, EPDM....................................................................................9611-99-35559611-99-35729611-99-3572 471O-ring, NBR (Standard).....................................................................9611-99-36379611-99-36419611-99-3645 1O-ring, EPDM....................................................................................9611-99-36369611-99-36409611-99-3644 1O-ring, HNBR....................................................................................9611-99-36399611-99-36439611-99-3647 1O-ring, FPM......................................................................................9611-99-36389611-99-36429611-99-3646 491Lip seal, NBR (Standard)....................................................................9613-0085-469613-0085-479613-0085-37 1Lip seal, EPDM.................................................................................9613-0085-269613-0085-319613-0085-36 1Lip seal, HNBR.................................................................................9613-0085-299613-0085-349613-0085-39 1Lip seal, FPM....................................................................................9613-0085-289613-0085-339613-0085-38 561Seal ring, NBR (Standard)..................................................................9613-0951-159613-0951-169613-0951-24 1Seal ring, EPDM................................................................................9613-0953-099613-0953-129613-0953-19 1Seal ring, HNBR................................................................................9613-0953-089613-0953-119613-0953-18 1Seal ring, FPM...................................................................................9613-0951-089613-0951-119613-0951-22 571Lip seal, NBR (Standard)...................................................................9613-0087-189613-0087-189613-0087-18 1Lip seal, EPDM.................................................................................9613-0087-119613-0087-119613-0087-11 1Lip seal, HNBR.................................................................................9613-0087-149613-0087-149613-0087-14 1Lip seal, FPM....................................................................................9613-0087-139613-0087-139613-0087-13 741Seal ring, NBR (Standard)..................................................................9613-0952-159613-0952-169613-0952-24 1Seal ring, EPDM................................................................................9613-0089-099613-0089-129613-0089-18 1Seal ring, HNBR................................................................................9613-0089-089613-0089-119613-0089-17 1Seal ring, FPM...................................................................................9613-0952-089613-0952-119613-0952-22 761O-ring, NBR (Standard)......................................................................9611-99-36379611-99-36419611-99-3645 1O-ring, EPDM....................................................................................9611-99-36369611-99-36409611-99-3644 1O-ring, HNBR....................................................................................9611-99-36399611-99-36439611-99-3647 1O-ring, FPM......................................................................................9611-99-36389611-99-36429611-99-3646 771Lip seal, NBR (Standard)...................................................................9613-0085-469613-0085-479613-0085-37 1Lip seal, EPDM.................................................................................9613-0085-269613-0085-319613-0085-36 1Lip seal, HNBR.................................................................................9613-0085-299613-0085-349613-0085-39 1Lip seal, FPM....................................................................................9613-0085-289613-0085-339613-0085-38Wear Parts3PartsReg.: 2009-01Intro.: 2009-014900-547PMO 2”PMO 2½”PMO 3”PMO 4”Pos.Qty.Denomination Seat ø81.3Seat ø115.3Seat ø115.3Seat ø143.9 1Complete actuator...............................................9613-0554-689613-0995-099613-0995-099613-0837-69 11Upper stem.........................................................9613-0101-029613-0074-019613-0074-019613-0074-02 24Screw..................................................................9611-99-33429611-99-33429611-99-3342 31Air fitting yellow....................................................9611-99-41719611-99-41719611-99-41719611-99-4171 1Air fitting blue......................................................9611-99-37809611-99-37809611-99-37809611-99-3780 1Air fitting red........................................................9611-99-41729611-99-41729611-99-41729611-99-4172 41Stop for upper piston..........................................9613-0053-019613-0053-019613-0053-02 51O-ring, NBR........................................................9611-99-34999611-99-34999611-99-34999611-99-3499 61Guide ring, Turcite...............................................9613-0084-089613-0084-089613-0084-089613-0084-08 71O-ring, NBR........................................................9611-99-35149611-99-35149611-99-3514 81Upper piston.......................................................9613-0056-019613-0056-019613-0056-02 91O-ring, NBR........................................................9611-99-35129611-99-35129611-99-3513 101Spring assembly..................................................9613-0125-019613-0075-019613-0075-019613-0256-03 111Distance spacer..................................................9613-0102-02121Pin......................................................................9611-99-41979611-99-41989611-99-41989611-99-4199 131Washer................................................................9611-99-35949611-99-35959611-99-35959611-99-3596 141Spring assembly..................................................9613-0131-029613-0095-029613-0095-029613-0095-02 151Plug....................................................................9613-4141-019613-4141-019613-4141-019613-4141-01 161Cylinder (3A marking)..........................................9613-0126-069613-0051-069613-0051-069613-0150-11 171Main piston.........................................................9613-0132-019613-0057-019613-0057-019613-0159-01 181Guide ring, Turcite...............................................9613-0084-099613-0084-109613-0084-109613-0084-11 191O-ring, NBR........................................................9611-99-35059611-99-35079611-99-35079611-99-3509 201O-ring, NBR........................................................9611-99-35039611-99-36079611-99-36079611-99-3607 211Bottom...............................................................9613-0140-019613-0054-019613-0054-019613-0168-01 221Guide ring, Turcite...............................................9613-0084-039613-0084-049613-0084-049613-0084-04 231O-ring, NBR........................................................9611-99-22289611-99-14899611-99-148922340675241Retaining ring......................................................9613-0248-029613-0248-039613-0248-039613-0248-04 251Cover disk...........................................................9613-0058-029613-0058-039613-0058-039613-0058-04 261O-ring, NBR........................................................9611-99-35289611-99-34959611-99-34959611-99-3530 271Inner stem...........................................................9613-0106-039613-0073-039613-0073-039613-0073-02 281O-ring.................................................................9611-99-34959611-99-00309611-99-00309611-99-0030 291Piston rod...........................................................9613-0134-029613-0060-029613-0060-029613-0060-02 301Lower piston.......................................................9613-0138-019613-0055-019613-0055-019613-0166-01 311O-ring, NBR.. (42153421534215342153)321Guide ring, Turcite................................................9613-0084-059613-0084-069613-0084-069613-0084-07 331O-ring, NBR........................................................223412759611-99-35089611-99-35089611-99-3510 343Bolt.....................................................................9611-99-36189611-99-36189611-99-36189611-99-3618 353Washer................................................................9611-99-35949611-99-35949611-99-35949611-99-3594 363Nut......................................................................9611-99-03609611-99-03609611-99-03609611-99-0360 421Spindle liner.........................................................9613-0335-019613-0090-019613-0090-019613-0090-01 432Clamp.................................................................9613-0336-019613-0092-019613-0092-019613-0092-01 441Lock....................................................................9613-0091-029613-0091-019613-0091-019613-0091-01 451Guide ring, PTFE.................................................9613-0084-149613-0084-159613-0084-159613-0084-21 481Upper sealing element.........................................9613-0064-049613-0188-049613-0188-049613-0713-01 541Guide ring, PTFE.................................................9613-0084-029613-0084-029613-0084-029613-0084-02 551Upper plug..........................................................9613-4586-019613-4587-019613-4587-039613-4588-01 612Wingnut...............................................................9612-5580-019612-5580-019612-5580-019612-5580-01 642Clamp without nut...............................................9613-0216-019613-0217-019613-0217-019613-0218-01 751Lower plug..........................................................9613-0705-019613-0706-019613-0707-019613-0708-01 791Lower sealing element.........................................9613-0241-049613-0243-049613-0243-049613-0715-01 801Guide ring, PTFE.................................................9613-0084-149613-0084-159613-0084-159613-0084-21 811Cover...................................................................9613-0490-069613-0490-079613-0490-079613-0490-16 821Bolt for indication.................................................9613-0926-029613-0926-019613-0926-019613-0926-01 831Sensor for indication............................................9611-99-45419611-99-45419611-99-45419611-99-4541 841Plate for sensor for indication...............................9613-0957-019613-0957-019613-0957-019613-0957-01Parts5Unique PMO Plus® Sanitary Mixproof ValveParts List/DrawingPartsPMO 2”PMO 2½”PMO 3”PMO 4”Pos.Qty.Denomination Seat ø81.3Seat ø115.3Seat ø115.3Seat ø143.9 371Intermediate piece......................................................9613-0191-219613-0192-159613-0192-139613-0193-17 501Valve body 11-00........................................................9613-0709-019613-0710-019613-0711-019613-0712-01 1Valve body 12-00........................................................9613-0709-059613-0710-059613-0711-059613-0712-05 1Valve body 21-00........................................................9613-0709-079613-0710-079613-0711-079613-0712-07 1Valve body 22-00........................................................9613-0709-099613-0710-099613-0711-099613-0712-09 1Valve body 11-90........................................................9613-0709-029613-0710-029613-0711-029613-0712-02 1Valve body 12-90........................................................9613-0709-069613-0710-069613-0711-069613-0712-06 1Valve body 21-90.......................................................9613-0709-089613-0710-089613-0711-089613-0712-08 1Valve body 22-90.......................................................9613-0709-109613-0710-109613-0711-109613-0712-10 1Valve body 11-180.....................................................9613-0709-039613-0710-039613-0711-039613-0712-03 1Valve body 11-270.....................................................9613-0709-049613-0710-049613-0711-049613-0712-04 900-547Intro.: 2009-0167Parts List/DrawingUnique PMO Plus ® Sanitary Mixproof ValveDenomination PMO 2”Service kit, NBR........................................................9611-92-6016Service kit, EPDM.....................................................9611-92-6013Service kit, HNBR.....................................................9611-92-6022Service kit, FPM........................................................9611-92-6019PMO 2.5”+3”Service kit, NBR........................................................9611-92-6017Service kit, EPDM.....................................................9611-92-6014Service kit, HNBR.....................................................9611-92-6023Service kit, FPM........................................................9611-92-6020PMO 4”Service kit, NBR........................................................9611-92-6018Service kit, EPDM.....................................................9611-92-6015Service kit, HNBR.....................................................9611-92-6024Service kit, FPM........................................................9611-92-6021(Plug set-up 12)TD 449-13955487579Service kits for Product Wetted Parts900-547Rebuilding Set PMO Plus® to PMO Plus® CPTD 449-362Reg.: 2009-01Intro.: 2009-0189PMO 2”PMO 2½”PMO 3”PMO 4”Seat ø81.3Seat ø115.3Seat ø115.3Seat ø143.9Lower plug, cpl., NBR..........................................................9613-4662-179613-4662-189613-4662-199613-4662-20Lower plug, cpl., EPDM.......................................................9613-4662-219613-4662-229613-4662-239613-4662-24Lower plug, cpl., HNBR.......................................................9613-4662-259613-4662-269613-4662-279613-4662-28Lower plug, cpl., FPM..........................................................9613-4662-299613-4662-309613-4662-319613-4662-32Rebuilding Set PMO Plus ® to PMO Plus ® CPPos.Qty.Denomination 381O-ring 741Seal ring 751Lower plug 761O-ring 951Special lip seal961Lower sealing element, upper part 971Lower sealing element, lower part 981Guide ringAxial Installation ToolReg.: 2008-01Intro.: 2008-011011Seat ø81.3Seat ø115.3Seat ø143.9Pos.Qty.Denomination 9613-0505-029613-0505-039613-0505-0711Lower part.........................................................21Piston................................................................31Upper part.........................................................41O-ring, NBR.......................................................9611-99-37039611-99-33499611-99-411351Clamp................................................................61Wingnut..............................................................71Air fitting.............................................................TD900198Axial Installation ToolRadial Installation ToolReg.: 2008-01Intro.: 2008-011213Seat ø81.3Seat ø115.3Seat ø143.9Pos.Qty.Denomination 9613-4260-029613-4260-049613-4260-0911Piston..........................................................21Lower part...................................................31Upper part...................................................41Bushing.......................................................51Guide..........................................................62Guide ring....................................................9613-0084-229613-0084-229613-0084-2371O-ring, NBR.................................................9611-99-33499611-99-33499611-99-411381O-ring, NBR.................................................9611-99-37059611-99-37059611-99-370591Clamp.........................................................101Wingnut.......................................................111Air fitting.......................................................TD900507Radial Installation Tool。
普洛麦格 (北京) 生物技术有限公司 RealTime-Glo
G9711, G9712 and G9713中文说明书适用产品目录号：G7130和G73602020版 CTB199原英文技术手册TB199DeadEnd™ ColorimetricTUNEL System普洛麦格(北京)生物技术有限公司Promega (Beijing) Biotech Co., Ltd 地址：北京市东城区北三环东路36号环球贸易中心B座907-909电话：************网址：技术支持电话：800 810 8133(座机拨打)，400 810 8133(手机拨打)技术支持邮箱：*************************CTB1992020制作1DeadEnd™ Colorimetric TUNEL System所有技术文献的英文原版均可在/ protocols获得。
请访问该网址以确定您使用的说明书是否为最新版本。
如果您在使用该试剂盒时有任何问题，请与Promega 北京技术服务部联系。
电子邮箱：*************************1.产品描述 (2)2.产品组分和储存条件 (4)3.检测步骤 (5)3.A. 用户需提供的材料和试剂制备 (5)3.B. 在组织切片上进行凋亡分析的步骤 (7)3.C. 在培养细胞中检测凋亡的步骤 (9)3.D. 阳性对照DNA酶处理步骤（可选） (10)3.E. 茴香霉素诱导的HL-60细胞凋亡步骤 (10)4.疑难解答 (11)5.缓冲液和溶液的成分 (12)6.相关产品 (13)7.参考文献 (14)8.内容变更总结 (15)普洛麦格(北京)生物技术有限公司Promega (Beijing) Biotech Co., Ltd 地址：北京市东城区北三环东路36号环球贸易中心B座907-909电话：************网址：技术支持电话：800 810 8133(座机拨打)，400 810 8133(手机拨打)技术支持邮箱：*************************CTB1992020制作21. 产品描述DeadEnd™ Colorimetric TUNEL System提供末端脱氧核苷酸转移酶（TdT）介导的断裂的核DNA缺口末端标记用试剂。
AdvanceBio SEC 130A
AdvanceBio SEC 130Å Protein Standard, Part Number 5190-9416*************(24小时)化学品安全技术说明书GHS product identifier 应急咨询电话（带值班时间）::供应商/ 制造商:安捷伦科技贸易（上海）有限公司中国（上海）外高桥自由贸易试验区英伦路412号（邮编:200131)电话号码： 800-820-3278传真号码: 0086 (21) 5048 2818AdvanceBio SEC 130Å Protein Standard, Part Number 5190-9416化学品的推荐用途和限制用途5190-9416部件号:安全技术说明书根据 GB/ T 16483-2008 和 GB/ T 17519-2013GHS化学品标识:AdvanceBio SEC 130Å 蛋白标样，部件号 5190-9416推荐用途3.5 mg, 冻干:有关环境保护措施，请参阅第 12 节。
物质或混合物的分类根据 GB13690-2009 和 GB30000-2013紧急情况概述固体。
[冻干]无资料。
无资料。
H320 - 造成眼刺激。
H334 - 吸入可能导致过敏或哮喘症状或呼吸困难。
物理状态:颜色:气味:GHS危险性类别警示词:危险危险性说明:H320 - 造成眼刺激。
H334 - 吸入可能导致过敏或哮喘症状或呼吸困难。
:防范说明标签要素混合物中由对水生环境毒性未知的组分组成的比率： 87.3%象形图皮肤腐蚀/刺激 - 类别 3严重眼损伤/眼刺激 - 类别 2B H334呼吸道致敏物 - 类别 1H317皮肤致敏物 - 类别 1P261 - 避免吸入粉尘。
P264 - 作业后彻底清洗。
P272 - 受沾染的工作服不得带出工作场地。
事故响应:P342 + P311 - 如有呼吸系统病症： 呼叫解毒中心或医生。
ADUM1301BRWZ中文资料
ENCODE ENCODE ENCODE
DECODE DECODE DECODE
16 VDD2 15 GND2 14 VOA 13 VOB 12 VOC
NC 6
11 NC
NC 7 GND1 8
10 VE2 9 GND2
Figure 1. ADuM1300 Functional Block Diagram
3 V operation 0.8 mA per channel maximum @ 0 Mbps to 2 Mbps 2.2 mA per channel maximum @ 10 Mbps 20 mA per channel maximum @ 90 Mbps
Bidirectional communication 3 V/5 V level translation High temperature operation: 125°C High data rate: dc to 90 Mbps (NRZ) Precise timing characteristics
元器件交易网
Triple-Channel Digital Isolators ADuM1300/ADuM1301
FEATURES
Automotive versions qualified per AEC-Q100 Low power operation
5 V operation 1.2 mA per channel maximum @ 0 Mbps to 2 Mbps 3.5 mA per channel maximum @ 10 Mbps 32 mA per channel maximum @ 90 Mbps
GENERAL DESCRIPTION
The ADuM130x1 are triple-channel digital isolators based on the Analog Devices, Inc., iCoupler® technology. Combining high speed CMOS and monolithic transformer technology, these isolation components provide outstanding performance characteristics superior to alternatives, such as optocouplers.
普洛麦格G9711 G9712 G9713 CTB288 中文说明书
G9711, G9712 and G97132021版 CTB288原英文技术手册TB288中文说明书适用产品目录号：G7570、G7571、G7572和G7573CellTiter-Glo® LuminescentCell Viability Assay普洛麦格(北京)生物技术有限公司Promega (Beijing) Biotech Co., Ltd 地址：北京市东城区北三环东路36号环球贸易中心B座907-909电话：************网址：技术支持电话：400 810 8133(手机拨打)技术支持邮箱：*************************CTB2882021制作1所有技术文献的英文原版均可在/ protocols 获得。
请访问该网址以确定您使用的说明书是否为最新版本。
如果您在使用该试剂盒时有任何问题，请与Promega 北京技术服务部联系。
电子邮箱：*************************1. 产品描述 (2)2. 产品组分和储存条件 (5)3. 进行CellTiter-Glo®检测 (6)3. A. 试剂制备 (6)3. B. Cell Viability Assay操作步骤 (7)3. C. 建立ATP标准曲线的操作步骤（可选） (7)4. 附录 (8)4. A. CellTiter-Glo® Assay概述 (8)4. B. 其他注意事项 (9)4. C. 参考文献 (11)4. D. 相关产品 (12)5. 内容变更总结 (14)CellTiter-Glo® Luminescent Cell Viability Assay普洛麦格(北京)生物技术有限公司Promega (Beijing) Biotech Co., Ltd 地址：北京市东城区北三环东路36号环球贸易中心B座907-909电话：************网址：技术支持电话：400 810 8133(手机拨打)技术支持邮箱：*************************CTB2882021制作21. 产品描述CellTiter-Glo® Luminescent Cell Viability Assay(a-d)是通过定量存在的ATP（它是新陈代谢活跃细胞存在的信号），测定培养物中活细胞数量的均质方法。
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1 3Project No.:Customer:Date: Prep. byChecked byDesignationDrawing No./EOrder No.tt-Part No.VS11Conveyor beltB00980757-00F251Equipment configuration00945071-00P301Base plate00932092-01L311Plate00942046-00L322Nozzle 10791740-02L332Clamp holder0794266-00L342Angle00903164-00L351Rail00932101-00L361Rail00932105-00L372Tab00940192-01L382Rework00940213-00L391Stud0794564-01L412Alignment pin0779109-00L512Compact cylinderADVU-20-25-P-A-S2156 0121103100-3-00LE545Sensorfor pneumatic cylinderSET00JK1260324-01LE552DU bushingø 10/12 x 15 mm1015 DU1102532-00LE618Hexagon socket head cap screwDIN 912 M5 x 40 - VZ 8.81100645-00L622Hexagon socket head cap screwDIN 912 M6 x 20 - VZ 8.81100657-00L634Hexagon socket head cap screwDIN 912 M8 x 25 - VZ 8.81100674-00L644Hexagon screwDIN 933 M8 x 30 - VZ 8.81100556-00L662Set screwDIN 913 M5 x 16 - VZ1100893-00LINDUSTRIE-STANDARDINDUSTRIE-STANDARDIHGINDUSTRIE-STANDARDINDUSTRIE-STANDARDINDUSTRIE-STANDARDFESTOBALLUFF29.12.2010grjAlteration:Item.Qty.ManufacturerFKILPBNQ01B00980155-Z01-01Designation:B00980155-01Cell magazineleft V3 for 5 carrierAssembly Drawing-No.:Page No.:of:DateName26.08.2010tlv
Assembly No.:003723.10.10.SA00101824-00Wuxi Jiacheng Solar Energy28.02.201219.
1
0.2010Machine No.:tlvAssembly Parts List
E= Spare partV= Wearing partS= Sep. spare part/wearing part listN= Refinishing workQ= Quality controlB= Place at disposatL= Stock componentP= PlanSeite 1 von 3I= Comm. Bosch comp.K= Purchased componentF= Production component 2 3Project No.:Customer:Date: Prep. byChecked byDesignationDrawing No./EOrder No.tt-Part No.VS29.12.2010grjAlteration:Item.Qty.ManufacturerFKILPBNQ01B00980155-Z01-01Designation:B00980155-01Cell magazineleft V3 for 5 carrierAssembly Drawing-No.:Page No.:of:DateName26.08.2010tlv
Assembly No.:003723.10.10.SA00101824-00Wuxi Jiacheng Solar Energy28.02.201219.
1
0.2010Machine No.:tlvAssembly Parts List
672Steel tube8.01 815 1 011010940239-00L682Clamping elementK2 00 18 CC S R 181318827-00LE692Clamping elementK8 01 18 CC S R12 R181318828-00LE712Cylindrical pinDIN 7979 D ø8 x 36DIN 7979 D ø8 x 361100751-00L724Locking pinDIN 7346 ø 10 x 301310727-00L732Hexagon screwDIN 933 M8 x 25 - VZ 8.81100555-00L751Proximity switchM12x1, inductive, s=6mmBES02WM1198852-00LE872Slotted orificehorizontalBAM00U010987508-00L881Light barrier0-10m, transmitter + receiverBOS01261313200-00LE902AccessoriesRod ø12, L=250mmBAM003910952740-00L1001Electr. cylinderNACH ANGEBOTNACH ANGEBOT 1093296610932966-00L1012Angled swivel screw connectionø6 - G1/81 823 391 2371100470-00L1044T-nutM8, groove 103 842 530 2871236616-01LFESTOBOSCH REXROTH PNEUBOSCH REXROTH IABALLUFFBALLUFFBALLUFFBALLUFFROSE+KRIEGERINDUSTRIE-STANDARDINDUSTRIE-STANDARDINDUSTRIE-STANDARDROSE+KRIEGERROSE+KRIEGER
E= Spare partV= Wearing partS= Sep. spare part/wearing part listN= Refinishing workQ= Quality controlB= Place at disposatL= Stock componentP= PlanSeite 2 von 3I= Comm. Bosch comp.K= Purchased componentF= Production component 3 3Project No.:Customer:Date: Prep. byChecked byDesignationDrawing No./EOrder No.tt-Part No.VS29.12.2010grjAlteration:Item.Qty.ManufacturerFKILPBNQ01B00980155-Z01-01Designation:B00980155-01Cell magazineleft V3 for 5 carrierAssembly Drawing-No.:Page No.:of:DateName26.08.2010tlv
Assembly No.:003723.10.10.SA00101824-00Wuxi Jiacheng Solar Energy28.02.201219.
1
0.2010Machine No.:tlvAssembly Parts List
1052T-nutM6, groove 83 842 501 7531107200-01L1068T-nutM5, groove 103 842 530 2831236614-01L50020Hexagon socket head cap screwDIN 912 M6 x 16 - VZ 8.81100656-00L5018Hexagon socket head cap screwDIN 912 M5 x 16 - VZ 8.81100640-00L5024Hexagon socket head cap screwDIN 912 M5 x 12 - VZ 8.81100639-00L5054Hexagon socket head cap screwDIN 912 M3 x 10 - VZ 8.81100622-00L5102Countersunk screwDIN 7991 M5 x 20 - VZ 8.81100575-00L5152Cylindrical pinDIN 7979 D ø8 x 32DIN 7979 D ø8 x 32
1100750-00L
5162Cylindrical pinDIN 7979 D ø6 x 16DIN 7979 D ø6 x 161100738-00L5204WasherDIN 125 B - 5,3 - VZ1100908-00L5951Cable ducts=130B60995102-01L6001Installation material=130B60980889-01FINDUSTRIE-STANDARD
INDUSTRIE-STANDARD
INDUSTRIE-STANDARDINDUSTRIE-STANDARD
INDUSTRIE-STANDARD
BOSCH REXROTH IAINDUSTRIE-STANDARD
INDUSTRIE-STANDARD
INDUSTRIE-STANDARD
BOSCH REXROTH IA
E= Spare partV= Wearing partS= Sep. spare part/wearing part listN= Refinishing workQ= Quality controlB= Place at disposatL= Stock componentP= PlanSeite 3 von 3I= Comm. Bosch comp.K= Purchased componentF= Production component