AP20187_HNMR_26011_MedChemExpress
2-[4-[(E)-(2-酮环己烯基)甲基]苯基]丙酸的晶型及其制备方法[发明专利]
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专利名称：2-[4-[（E）-（2-酮环己烯基）甲基]苯基]丙酸的晶型及其制备方法
专利类型：发明专利
发明人：袁威冠,宁虎林,谭军华,王谦志,肖稳定
申请号：CN201911362702.3
申请日：20191226
公开号：CN111039782A
公开日：
20200421
专利内容由知识产权出版社提供
摘要：本发明公开了一种新的2‑[4‑[（E）‑（2‑酮环己烯基）甲基]苯基]丙酸的晶型，填补了目前无晶型报道的空白。
且此晶型不易吸湿、在高温、高湿条件下也很稳定，使得药物的安全性进一步得到了提高，方便了后续的制剂过程。
申请人：湖南九典制药股份有限公司
地址：410329 湖南省长沙市浏阳经济技术开发区健康大道1号
国籍：CN
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高效液相色谱法检测XXX注射液药包材浸出物抗氧剂1010和3-（3,5-二叔丁基-4-羟基苯基）丙酸
高效液相色谱法检测XXX注射液药包材浸出物抗氧剂1010和3-（3,5-二叔丁基-4-羟基苯基）丙酸摘要：本研究采用高效液相色谱检测XXX注射液中包装材料浸出物抗氧剂1010和3-（3,5-二叔丁基-4-羟基苯基）丙酸。
用Poroshell 120 EC-C18为固定相，以乙腈-水（含0.1%冰乙酸）为流动相的梯度洗脱，以280nm的波长，300nm的参比波长的二极管阵列检测器进行检测。
结果表明，抗氧剂1010，3-（3,5-二叔丁基-4-羟基苯基）丙酸分别在1.835～45.001μg/ml和0.601～7.700μg/ml范围内浓度与峰面积呈现良好的线性关系，在低、中、高三个加标浓度下的回收率分别为100.90%～106.57%和99.64～107.62%，精密度RSD（n=6）分别为0.34%和0.96%。
在XXX注射液药包材中抗氧剂1010和3-（3,5-二叔丁基-4-羟基苯基）丙酸的检测限分别为0.915μg/ml、0.302μg/ml。
关键词：抗氧剂1010、3-（3,5-二叔丁基-4-羟基苯基）丙酸、药包材相容性、浸出物，高效液相色谱引言抗氧剂1010是一种高分子量的受阻酚抗氧剂，挥发性很低，而且不易迁移，耐萃取，广泛应用于通用塑料，合成橡胶、墨水等行业中。
在药品与包装材料长期接触过程中，抗氧剂1010存在从包材中迁移至药品中的潜在风险，《化学药品与弹性体密封件相容性研究指导原则》1和《化学药品注射剂与塑料包装材料相容性研究指导原则》2均明确将其列为需要关注的物质。
抗氧剂1010在与药品长期作用中可能发生水解，产生3-（3,5-二叔丁基-4-羟基苯基）丙酸，后者的分子量更小，水溶性更好，可迁移性更强。
3这两种物质作为常见的浸出物对药品的安全属性造成潜在危害，需进行检测评估。
本文采用高效液相色谱对抗氧剂1010和3-（3,5-二叔丁基-4-羟基苯基）丙酸进行检测，建立了有效的分析方法，并对方法的检测限、精密度、回收率进行了评估，为注射剂与塑料包装材料相容性研究工作提供技术支撑。
试管扩散法检测牛乳中β-酰胺类抗生素残留研究
试管扩散法检测牛乳中β-酰胺类抗生素残留研究
吴瑕;邵辉;王鑫;张兰威
【期刊名称】《东北农业大学学报》
【年(卷),期】2011(042)005
【摘要】试管扩散法是以微生物受阻法为基本原理.用嗜热脂肪芽孢杆茵作为指示茵.在培养基中加入指示剂,根据嗜热脂肪芽孢杆茵在生乳中生长产酸使指示剂变色现象,确定牛乳中β-内酰胺类抗生素残留量,比较试管扩散法和TTC法检测牛乳中6种β-内酰胺类抗生素残留得到的检测限和检测时间等指标.结果表明,试管扩散法比TTC法更适合国内牛乳中β-内酰胺类抗生素残留的检测.
【总页数】6页(P31-35,后插一)
【作者】吴瑕;邵辉;王鑫;张兰威
【作者单位】东北农业大学成栋学院,哈尔滨150030;黑龙江龙丹乳业有限公司,哈尔滨,150046;东北农业大学成栋学院,哈尔滨150030;哈尔滨工业大学食品科学与遗传工程研究院,哈尔滨150001
【正文语种】中文
【中图分类】TS252.1
【相关文献】
1.试管扩散法检测牛乳中青霉素G残留量 [J], 吴瑕;张兰威
2.试管扩散法与国标TTC法检测牛乳中β-内酰胺类抗生素残留 [J], 李延华;王伟军;张兰威;陈丽安;马薇
3.嗜热脂肪芽孢杆菌试管法检测牛乳中β-内酰胺类抗生素残留 [J], 王伟军;李延华;张兰威;马微
4.牛乳中β-内酰胺类抗生素残留检测方法的对比研究 [J], 李延华;王伟君;张兰威
5.微生物法检测牛乳中β-内酰胺类抗生素残留的对比研究 [J], 李延华;王伟军;张兰威;马微;陈丽安
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Presatovir产品说明书
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Separate eyelids with fingers to ensure adequate flushing. Promptly call a physician. Skin contactSafety Data SheetRevision Date:Jun.-3-2021Print Date:Feb.-17-2023Inhibitors •Screening Libraries•ProteinsRinse skin thoroughly with large amounts of water. Remove contaminated clothing and shoes and call a physician.InhalationImmediately relocate self or casualty to fresh air. If breathing is difficult, give cardiopulmonary resuscitation (CPR). Avoid mouth-to-mouth resuscitation.IngestionWash out mouth with water; Do NOT induce vomiting; call a physician.4.2 Most important symptoms and effects, both acute and delayedThe most important known symptoms and effects are described in the labelling (see section 2.2).4.3 Indication of any immediate medical attention and special treatment neededTreat symptomatically.5. 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科玛嘉显色培养基在念珠菌鉴定中的应用价值探讨
科玛嘉显色培养基在念珠菌鉴定中的应用价值探讨影像与检验CHINAFOREIGNMEDICAL墨固科玛嘉显色培养基在念珠菌鉴定中的应用价值探讨黄峰秦淑国边其侠(安徽省宿州市皖北煤电集团总医院检验科安徽宿州234000)【摘要】目的评价科玛嘉显色培养基在念珠茵鏊定中的应用价值.方法用法国生物梅里埃公司生产的ATBID32C鉴定卡和科玛嘉显色培养基同时鉴定190株念珠茵.结果190株念珠茼中,科玛嘉显色培养基鉴定出白色念球菌127株,光滑念珠茵35株,热带念珠茵21株,克柔念珠茵2株.ID32C鉴定卡鉴定出白色念珠茵127株,光滑念珠茵36株,热带念珠茵22株,克柔念珠茵2株.结论科玛嘉显色培养基在念珠茵鉴定中符合率高,方法简便,用时较短且价格便宜,能快速准确地整定出临床常见念珠茵.ID32C鉴定卡能将念球菌全部鉴定到种,但价格较贵,且用时较长.【关键词l科玛嘉显色培养基念珠茵【中图分类号】R37【文献标识码】A【文章编号】1674--0742(2009)02(b)--0149--02 近年来由于高效广谱抗生素,肾上腺皮质激素,免疫抑制剂,恶性肿瘤化疗及放疗,多种侵袭性操作手段的广泛应用,导致菌群失调的真菌感染日益增多,因此及时准确的培养及鉴定出念珠菌,成为了微生物室急需要解决的重要问题.科玛嘉显色培养基是通过培养基上产生颜色差异和菌落特征来进行快速鉴定念珠菌的培养基,我们将此培养基与ATBexpression自动细菌鉴定/药敏系统的配套ATBID32C鉴定卡作了如下对比.1材料与方法1.1标本我院门诊与住院患者真菌培养标本1356份.1.2培养基和试剂沙保罗培养基(SDA)干粉购自杭州天和微生物试剂有限公司,科玛嘉显色培养基干粉购自郑州博赛生物技术研究所.ATBID32C鉴定卡为法国生物梅里埃公司产品.1.3仪器法国梅里埃ATBexpression自动细菌鉴定/药敏系统;30~C孵育箱.1.4方法表1190酵母样真菌分类结果名称标本直颈分泌物痰p段尿其他合计(%)将SDA~N科玛嘉显色培养基按常规制成培养基,各类标本按常规同时分区划线接种于SDA培养基和科玛嘉显色培养基,30~C培养24～4gh后观察结果,在两种培养基上7d不生长判为阴性.(1)在SDA培养基上培养阳性者经革兰染色确认为酵母样菌后,严格按生物梅里埃ATBID32C的鉴定系统操作,24～48h后用ATBexpression自动细菌鉴定/药敏系统测定结果.(2)在科玛嘉显色培养基上生长为翠绿色菌落(直径约2ram)为白色念珠菌,蓝灰色菌落(直径约1.5mm)为热带念珠菌,紫红色边缘模糊有微毛(直径约4～5mm)为克柔念珠菌,整个菌落紫红色(直径约2ram)为光滑念珠菌,白色为其他念珠菌.2结果(1)1356份标本在SDA上培养出190株酵母菌,在SDA上用A TBID32C鉴定卡鉴定分类结果见表1,在科玛嘉显色培养基上鉴定分类结果见表2.(2)190株科玛嘉显色培养基可鉴定株为185株,占总株数的97.4%,而ATBID32C与科玛嘉显色培养基直接鉴定法鉴定符合率为98.4%.3讨论(1)本文的检测结果表明,白色念珠菌占酵母样真菌数量的66.8‰光滑念珠菌占18.4%,热带念珠菌占l1.1%,克柔念珠菌占1.1%,这4种念珠菌占总数的97.4%,由此可以看出,本地区的念珠菌感染还是以白色念珠菌为主,但是光滑念珠菌感染已经上升到第2位,热带念珠菌感染所占的比例有所下降.(2)由于科玛嘉显色培养基对于不同酵母菌菌落显现不同颜色及形态差别,据此可对常见四种念珠菌作出初步判断,对于白色念珠菌,光滑念珠菌,热带念珠菌,克柔念珠菌与ATBID32C鉴定符合率达到了98.4%,差异无显着性(,0.05),表明这与许宏涛报道的结果接近….(3)在实际工作中,我们发现,科玛嘉显色培养基能在24～72h鉴定出4种常见的念珠菌,尤其是白色念珠菌,克柔念珠菌,他们的颜色和菌落特征明显,能够快速准确地得到鉴定(24-48h),而光滑念珠菌,热带念珠菌有时则需要更长一点的时间,使菌落颜色得到充分的表现,才能得到准确的判断(48～72h).A TBID32C法鉴定的结果很准确,但其价格较高,且分离培养需要24-48h,接种ID32C鉴定板条后需再培养24-48h,方能得出结果,所需时间相对较长,并且需要配套的仪器,而应用科玛嘉显色培养基不仅缩短了检测时间,而且价袼适CHINAFOREIGNMEDICALTREA TMENT中罗医疗149影像与检验高密度脂蛋白胆固醇的直接测定法研究谢智光(广西中医学院附属瑞康医院检验科广西南宁530011)【摘要】目的建立高密度脂蛋白胆固醇直接测定法法.方法用甾类糖苷化合物与胆固醇醴酶和胆固醇氧化酶制备的高亲和性酶化合物,结合特殊表面活性刺,通过对测定条件的优化,实现HD1-_C的直接测定.结果本方法与磷鸪酸镁(PTA—Mg2+)沉淀法…和葡聚糖一氯化镁(DS50--Mg)~淀结合ALBK法口相关性良好,分别为,=0.990I,Y=1.0l6X-O.0818和,=0.9960.Y=1.008X一0.063.批内cV<1.B%,日问cV<2.1%,线性范围逸5.4mmol/L,回收率!oo±5%.TG浓度迭30mmol/L,抗坏血酸<3.5mmol/1,血缸蛋白<4.8g/L和胆红素<540mol/L时无显着干扰.当用纯的不同浓度的LDL加入准确定值的新鲜血清中,观察脂蛋白在血清中的反应.结果纯LDL—C浓度在10.Ommol/L以内对本法无显着干扰.结论本文建立的高密度脂蛋白胆固醇直接法方法,其性能指标符合临床使用要求.标本无需预处理,精密度好,准确性高,适用于各种自动生化分析仅.【关键词l高密度脂蛋白胆固醇直接测定法【中图分类号lR44【文献标识码lA【文章编号l1674--0742(2009)o2(b)一ol50-02 近年来流行病学及大规模临床研究已经证实高密度脂蛋白(HDL)对动脉血管壁有直接的保护作用,并能促进动脉粥样斑快的消退,甚至部分研究发现高密度脂蛋白胆固醇(HDL—c)较LDL—C能更好地预测冠心病的危险】.高密度脂蛋白胆固醇(HDL—C)的直接测定法有不需要血清标本预处理的优点,适于直接上自动生化分析仪检测,目前已为各大医院所采用,也是今后发展的趋势.我们采用甾类糖苷化合物与胆固醇酯酶和胆固醇氧化酶制备的高亲和性酶化合物,结合对高密度脂蛋白胆固醇具有较强作用的表面活性剂研制的直接测定HDL-C~/d的工作已获得成功,现报道如下.1材料与方法1.1仪器.罗氏全自动分析仪1.2试剂试剂I:缓冲液,高亲合酶胆固醇酯酶化合物,高亲合酶胆固醇氧化酶化合物,辣根过氧化物酶,抗坏血酸氧化酶,HDAOS,稳定剂和防腐剂;试剂II:缓冲液,4一氨基安替比林,表面活性剂,稳定剂和防腐剂.校准血清为:HBS抗原阴性,HCV和HIV阴性,无脂浊,黄疸,溶血的键康体检者的新鲜混合人血清,1mL分装冻干,然后用DS50一Mg法进行定值.1.3测定方法HITACHI7060型自动分析仪参数:反应类型:二点终点法;反应温度:3712;波长:(主)546/(次)700nml样品:3L,JJ~R1180"L,3～5min读取空白读数(A1),然后加R260L,5min后读取测定读数(A2).1.4比较方法磷钨酸镁(PTA—Mg)沉淀法和葡聚糖(DS50)-氯化镁沉淀法.2实验结果2.1特异性试验取新鲜混合血清对HDL—C进行定值,浓度为1.75mmol/L;将其分成9份,分别加入超速离心分离的LDL纯品组份,加入的LDL-C的量分别为1.2,2.4,3.6,4.8,6.0,7.2,8.4和9.4mmol/L,然后分别用本法测定上述9份样品的HDL-C值,经校准加入LDL组份的稀释因素,结果说明加入LDL—C达10.0mmol/L并不影响HDL—C的测定结果.2.2线性范围中,操作简便,适合我国大,中,小型实验室使用l2】.(4)对于混合念珠菌感染的判断,科玛嘉显色培养基显示了极大的优势,两种或多种念珠菌混合感染均可作出明确判断口l.(5)另外,我们在日常的念珠菌培养试验中发现,在科玛嘉显色培养基生长的念珠菌,将其用ATBID32C鉴定卡进行试验并不影响结果的确认,有关文献对此曾有报道NI.因此,有条件的医院可以采用科玛嘉显色培养基与仪器法结合的方法,真菌培养的标本可以直接接种科玛嘉显色培养基,这样可以快速,简便地分离鉴定出大部分临床常见的念珠菌,对于少数不能显色或显色不充分有疑问的菌株可以用仪器鉴定,这样既可以降低成本,又可以提高鉴定的准确性.参考文献【1】许宏涛,张秀珍.科玛嘉念珠菌显色培养基的应用【J】.中华检验医学杂志,2000,23(5):298～299.15O中岁医疗CHINAFOREIGNMEDICALTREATMENT【2]周广,谢元宏,王知秋.科玛嘉显色培养基与API鉴定念珠菌的分析比较【J】.Jll;lls医学院学报,2004,19(1).[3】周燕等.科玛嘉念珠菌显色培养基与沙保罗培养基的应用比较【J】.现代检验医学杂志,2005,5:54.【4】朱成宾,夏永祥,窦露.科玛嘉念珠菌显色培养基的临床应用与评价【J】.实用医技杂志,2003,10(6).【收稿日期】2008—11—12。
用于药物中大肠杆菌、沙氏门菌检测的引物组、试剂盒及检测方法[
六年级上册复习计划六年级上册即将结束，复习计划的制定显得尤为重要。
为了让同学们在期末考试中取得优异成绩，下面将详细介绍六年级上册的复习计划安排。
语文复习语文是基础学科，也是六年级上册的必修科目之一。
首先，同学们应该把课本重点内容进行整理，特别是课文中的生字词、语法知识和修辞手法等方面。
其次，要做好阅读理解和写作练习，提高语文综合能力。
最后，要重点复习古诗文、议论文等文体，做到知识点明晰，理解透彻。
数学复习数学作为一门理科学科，需要同学们掌握扎实的基础知识。
在复习过程中，同学们应该重点攻克四则混合运算，各种图形的周长和面积计算，以及分数、小数等知识点。
此外，还要多做题，提高解题能力和应变能力。
记得要及时整理错题，找出问题所在，及时订正。
英语复习英语作为一门外语，需要同学们大量的词汇积累和语法练习。
在复习英语过程中，同学们要注重听说读写的综合能力，多听力训练，多背单词，多模仿口语。
同时，要熟悉各种句型和语法规则，做到灵活运用。
建议同学们多参加英语角、英语演讲比赛等活动，提高英语应用能力。
综合复习除了单科复习外，同学们还应进行综合复习。
这就要求同学们要制定详细的学习计划，合理安排时间，做到科学高效。
可以采取制作时间表、利用碎片时间、结合游戏学习等方法，让学习变得更加有趣。
在复习过程中，同学们要有耐心和毅力，保持良好的学习状态，做到持之以恒。
总结六年级上册的复习计划制定是一个循序渐进的过程，需要同学们严格执行，做到有的放矢。
只有在平时的努力和积累下，才能在期末考试中取得优异成绩。
希望同学们能够认真学习，按照复习计划的安排行动，取得令人满意的成绩。
祝愿同学们复习顺利，考试成功！。
AP20187_DataSheet_MedChemExpress
Inhibitors, Agonists, Screening Libraries Data SheetBIOLOGICAL ACTIVITY:AP20187 is a cell–permeable molecule used to dimerize FK506–binding protein (FKBP ) fusion proteins and initiate biological signaling cascades and gene expression or disrupt protein–protein interactions.IC50 & Target: FKBP homodimerizer [1]In Vitro: When LNCaP cells are treated with AP20187 (100 nM), ro–iCaspase–9 levels are significantly reduced, and the smaller processed active caspase–9 becomes apparent [2].In Vivo: Real–time PCR analysis shows that AP20187 (0.5 mg/kg, 2 mg/kg, or 5 mg/kg) treatment significantly increases the levels of CHOP mRNA in the CNS of PLP/Fv2E–PERK mice at PID12. AP20187 treatment significantly alleviates EAE–induced myelin damage in these mice. AP20187 treatment significantly reduces the number of degenerating axons and increases the density of axons in the demyelinating lesions in the lumbar spinal cord of PLP/Fv2E–PERK mice [2].PROTOCOL (Extracted from published papers and Only for reference)Cell Assay: AP20187 is dissolved in PBS [2].[2]For the in vitro study, 16 h after ADV infection, cells are treated with R1881 (10 nM),AP20187 (10 nM), both, or neither for 8 h. Cells are then rinsed with PBS and fixed with 4% paraformaldehyde for 1 h at room temperature. After rinsing with PBS, cells are incubated in ice–cold permeabilization solution (0.1% Triton X–100, 0.1% sodium citrate) for 2 min at 0°C. Cells are rinsed with PBS and stained with TUNEL reaction mixture for 60 min at 37°C. After another PBS wash, cells are incubated with Converter–AP for 30 min at 37°C. Cells are rinsed and incubated with substrate5–bromo–4–chloro–3–indolyl phosphate/nitroblue tetrazolium for 30 min. After a final PBS rinse (repeated twice), cells are microphotographed [2].Animal Administration: AP20187 is dissolved in 100% ethanol at a concentration of 62.5 mg/mL and stored (20°C). Then treatmentsolutions is freshly prepared from a dilution of 1.25 mg/mL consisting of 4% ethanol, 10% PEG–400, and 2% Tween–20 in water [2]. [2]Mice [2]To activate the transgene Fv2E–PERK in oligodendrocytes, PLP/Fv2E–PERK transgenic mice are given intraperitoneal injections of AP20187 daily at a dose of 0.5 mg/kg, 2 mg/kg, or 5 mg/kg. Lyophilized AP20187 is dissolved in 100% ethanol at a concentration of 62.5 mg/mL stock solution and stored at -20°C. Injection solutions consist of 4% ethanol, 10% PEG–400, and 2% Tween–20 in water.The transgenic mice receiving only the vehicle (4% ethanol, 10% PEG–400, 2% Tween–20 in water) served as controls.References:[1]. Ahmed S, et al. Photocleavable dimerizer for the rapid reversal of molecular trap antagonists. J Biol Chem. 2014 Feb 21;289(8):4546–52.[2]. Lin W, et al. Oligodendrocyte–specific activation of PERK signaling protects mice against experimental autoimmune encephalomyelitis. J Neurosci. 2013Product Name:AP20187Cat. No.:HY-13992CAS No.:195514-80-8Molecular Formula:C 82H 107N 5O 20Molecular Weight:1482.75Target:mTOR Pathway:PI3K/Akt/mTOR Solubility:DMSO: ≥ 57 mg/mLApr 3;33(14):5980–91.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。
超高效液相色谱法测定垂盆草颗粒中3种黄酮成分的含量
超高效液相色谱法测定垂盆草颗粒中3种黄酮成分的含量华燕青;张楠【摘要】目的建立超高效液相色谱法(UPLC)同时测定垂盆草颗粒中槲皮素、山柰素与异鼠李素的含量.方法色谱柱Thermo Scientific AcclaimTM RSLC 120C18(2.1 mm×100 mm,2.2 μm),流动相为甲醇-0.4％磷酸(50∶50),柱温为30℃,流速为0.3 mL·min-1,检测波长为360 nm,进样量1μL.结果槲皮素、山柰素与异鼠李素的线性范围分别为:50～1 000 μg,20～400 μg,20 ～400 μg,且与峰面积呈良好线性关系(r=0.999 9,0.999 9,0.999 6),精密度、稳定性、重复性试验的RSD 均＜2％,平均加样回收率(n=9)分别为99.35％,99.38％,98.91％,RSD分别为1.04％、0.98％、1.01％.结论该法高效、准确、灵敏,可作为测定垂盆草颗粒中槲皮素、山柰素与异鼠李素含量的有效方法.【期刊名称】《安徽医药》【年(卷),期】2018(022)008【总页数】4页(P1457-1460)【关键词】超高效液相色谱法;垂盆草颗粒;含量测定;槲皮素;山柰素;异鼠李素【作者】华燕青;张楠【作者单位】杨凌职业技术学院,陕西杨凌712100;西安市食品药品检验所,陕西西安710065【正文语种】中文垂盆草(Sedum sarmentosum Bunge)为景天科植物，全草入药，具有清热解毒，活血利湿的功效，可用于治疗带状疱疹，毒蛇咬伤、烫伤、烧伤及急慢性肝炎湿热淤结症[1]。
垂盆草中富含垂盆草苷、黄酮类、三萜类、生物碱等多种化学成分[2]。
研究表明[3-4]，垂盆草总黄酮是垂盆草中重要的保肝降酶作用的活性成分之一，垂盆草总黄酮中含有槲皮素、山柰素、木犀草素、苜蓿苷、槲皮苷、金丝桃苷、木犀草苷、异鼠李素等多种黄酮类化合物。
垂盆草颗粒为现行2015年版药典一部收载品种[5]，其含量测定采用紫外-可见分光光度法，以芦丁为对照测定了垂盆草颗粒中总黄酮含量。