LSD-TEST3S9B96-01_V1.0

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【1】EDC的M3资源介绍_讲稿

项目型号
产品名称
产品型号
LSD-S2E21-01 串口转以太网转换板（独立模块） LSD-S2E21-01
嵌入式GPRS数据传输模块
简介：
嵌入式GPRS数据传输模块是利尔达公司自行设计研发的基于 Cortex M3内核的远程数据传输模块，具有使用方便，配置简单，传输高效，性能稳定等优点。该模块提供RS232和RS485两路串口进行数据传输和配置接口，模块接到数据后通过GPRS网络将数据传输到远程服务器上，在电力系统，工业自动化、设备监控、金融、交通管理、环保监测、气象、煤矿等行业广泛应用。
项目型号
LSD-TEST3S3748
产品名称
产品型号
嵌入式LM3S3748学习板 LSD-TEST3S3748
嵌入式LM3S8962学习板
简介：该学习板支持Ethernet+CAN LM3S8962和CANLM3S2110，可实现完整CAN网络。包含开源FreeRTOS操作系统以及LwIP和uIP以太网协议栈的嵌入式Web服务器。板载FTDI仿真器，提供对外仿真的功能。特点：一体化全功能Ethernet+CAN，包含完整实例程序 LM3S8962 Ethernet主板和LM3S2110 CAN设备板集成10/100（MAC+PHY）以太网控制器两块Stellaris器件都全面集成了CAN的MAC层 USB线缆提供串行通信、调试功能和电源 128X64像素分辨率和16级灰度的OLED图形显示屏用户LED、导航开关、选择按钮和电磁扬声器所有LM3S8962的I/O段口外扩输入和输出的标准ARM20引脚JTAG/SWD调试连接器 MicroSD卡插槽支持FAT32文件系统
特点：

NMR Solvent

show their degree of variability.Occasionally,in order to distinguish between peaks whose assignment was ambiguous,a further1-2μL of a specific substrate were added and the spectra run again.Table1.1H NMR Dataproton mult CDCl3(CD3)2CO(CD3)2SO C6D6CD3CN CD3OD D2O solvent residual peak7.26 2.05 2.507.16 1.94 3.31 4.79 H2O s 1.56 2.84a 3.33a0.40 2.13 4.87acetic acid CH3s 2.10 1.96 1.91 1.55 1.96 1.99 2.08 acetone CH3s 2.17 2.09 2.09 1.55 2.08 2.15 2.22 acetonitrile CH3s 2.10 2.05 2.07 1.55 1.96 2.03 2.06 benzene CH s7.367.367.377.157.377.33tert-butyl alcohol CH3s 1.28 1.18 1.11 1.05 1.16 1.40 1.24 OH c s 4.19 1.55 2.18tert-butyl methyl ether CCH3s 1.19 1.13 1.11 1.07 1.14 1.15 1.21 OCH3s 3.22 3.13 3.08 3.04 3.13 3.20 3.22 BHT b ArH s 6.98 6.96 6.877.05 6.97 6.92OH c s 5.01 6.65 4.79 5.20ArCH3s 2.27 2.22 2.18 2.24 2.22 2.21ArC(CH3)3s 1.43 1.41 1.36 1.38 1.39 1.40chloroform CH s7.268.028.32 6.157.587.90 cyclohexane CH2s 1.43 1.43 1.40 1.40 1.44 1.451,2-dichloroethane CH2s 3.73 3.87 3.90 2.90 3.81 3.78 dichloromethane CH2s 5.30 5.63 5.76 4.27 5.44 5.49diethyl ether CH3t,7 1.21 1.11 1.09 1.11 1.12 1.18 1.17 CH2q,7 3.48 3.41 3.38 3.26 3.42 3.49 3.56 diglyme CH2m 3.65 3.56 3.51 3.46 3.53 3.61 3.67 CH2m 3.57 3.47 3.38 3.34 3.45 3.58 3.61OCH3s 3.39 3.28 3.24 3.11 3.29 3.35 3.37 1,2-dimethoxyethane CH3s 3.40 3.28 3.24 3.12 3.28 3.35 3.37 CH2s 3.55 3.46 3.43 3.33 3.45 3.52 3.60 dimethylacetamide CH3CO s 2.09 1.97 1.96 1.60 1.97 2.07 2.08 NCH3s 3.02 3.00 2.94 2.57 2.96 3.31 3.06NCH3s 2.94 2.83 2.78 2.05 2.83 2.92 2.90 dimethylformamide CH s8.027.967.957.637.927.977.92 CH3s 2.96 2.94 2.89 2.36 2.89 2.99 3.01CH3s 2.88 2.78 2.73 1.86 2.77 2.86 2.85 dimethyl sulfoxide CH3s 2.62 2.52 2.54 1.68 2.50 2.65 2.71 dioxane CH2s 3.71 3.59 3.57 3.35 3.60 3.66 3.75 ethanol CH3t,7 1.25 1.12 1.060.96 1.12 1.19 1.17 CH2q,7d 3.72 3.57 3.44 3.34 3.54 3.60 3.65OH s c,d 1.32 3.39 4.63 2.47ethyl acetate CH3CO s 2.05 1.97 1.99 1.65 1.97 2.01 2.07C H2CH3q,7 4.12 4.05 4.03 3.89 4.06 4.09 4.14CH2C H3t,7 1.26 1.20 1.170.92 1.20 1.24 1.24 ethyl methyl ketone CH3CO s 2.14 2.07 2.07 1.58 2.06 2.12 2.19C H2CH3q,7 2.46 2.45 2.43 1.81 2.43 2.50 3.18CH2C H3t,7 1.060.960.910.850.96 1.01 1.26 ethylene glycol CH s e 3.76 3.28 3.34 3.41 3.51 3.59 3.65“grease”f CH3m0.860.870.920.860.88CH2br s 1.26 1.29 1.36 1.27 1.29n-hexane CH3t0.880.880.860.890.890.90CH2m 1.26 1.28 1.25 1.24 1.28 1.29HMPA g CH3d,9.5 2.65 2.59 2.53 2.40 2.57 2.64 2.61 methanol CH3s h 3.49 3.31 3.16 3.07 3.28 3.34 3.34 OH s c,h 1.09 3.12 4.01 2.16nitromethane CH3s 4.33 4.43 4.42 2.94 4.31 4.34 4.40 n-pentane CH3t,70.880.880.860.870.890.90CH2m 1.27 1.27 1.27 1.23 1.29 1.292-propanol CH3d,6 1.22 1.10 1.040.95 1.09 1.50 1.17 CH sep,6 4.04 3.90 3.78 3.67 3.87 3.92 4.02 pyridine CH(2)m8.628.588.588.538.578.538.52 CH(3)m7.297.357.39 6.667.337.447.45CH(4)m7.687.767.79 6.987.737.857.87 silicone grease i CH3s0.070.130.290.080.10 tetrahydrofuran CH2m 1.85 1.79 1.76 1.40 1.80 1.87 1.88 CH2O m 3.76 3.63 3.60 3.57 3.64 3.71 3.74 toluene CH3s 2.36 2.32 2.30 2.11 2.33 2.32CH(o/p)m7.177.1-7.27.187.027.1-7.37.16CH(m)m7.257.1-7.27.257.137.1-7.37.16 triethylamine CH3t,7 1.030.960.930.960.96 1.050.99 CH2q,7 2.53 2.45 2.43 2.40 2.45 2.58 2.57a In these solvents the intermolecular rate of exchange is slow enough that a peak due to HDO is usually also observed;it appears at2.81and3.30ppm in acetone and DMSO,respectively.In the former solvent,it is often seen as a1:1:1triplet,with2J H,D)1Hz. b2,6-Dimethyl-4-tert-butylphenol.c The signals from exchangeable protons were not always identified.d In some cases(see note a),the coupling interaction between the CH2and the OH protons may be observed(J)5Hz).e In CD3CN,the OH proton was seen as a multiplet atδ2.69,and extra coupling was also apparent on the methylene peak.f Long-chain,linear aliphatic hydrocarbons.Their solubility in DMSO was too low to give visible peaks.g Hexamethylphosphoramide.h In some cases(see notes a,d),the coupling interaction between the CH3and the OH protons may be observed(J)5.5Hz).i Poly(dimethylsiloxane).Its solubility in DMSO was too low to give visible peaks.Notes .Chem.,Vol.62,No.21,19977513.Chem.,Vol.62,No.21,1997NotesTable2.13C NMR Data aCDCl3(CD3)2CO(CD3)2SO C6D6CD3CN CD3OD D2O solvent signals77.16(0.0629.84(0.0139.52(0.06128.06(0.02 1.32(0.0249.00(0.01206.26(0.13118.26(0.02acetic acid CO175.99172.31171.93175.82173.21175.11177.21 CH320.8120.5120.9520.3720.7320.5621.03 acetone CO207.07205.87206.31204.43207.43209.67215.94 CH330.9230.6030.5630.1430.9130.6730.89 acetonitrile CN116.43117.60117.91116.02118.26118.06119.68 CH3 1.89 1.12 1.030.20 1.790.85 1.47 benzene CH128.37129.15128.30128.62129.32129.34tert-butyl alcohol C69.1568.1366.8868.1968.7469.4070.36 CH331.2530.7230.3830.4730.6830.9130.29 tert-butyl methyl ether OCH349.4549.3548.7049.1949.5249.6649.37 C72.8772.8172.0472.4073.1774.3275.62C C H326.9927.2426.7927.0927.2827.2226.60 BHT C(1)151.55152.51151.47152.05152.42152.85C(2)135.87138.19139.12136.08138.13139.09CH(3)125.55129.05127.97128.52129.61129.49C(4)128.27126.03124.85125.83126.38126.11CH3Ar21.2021.3120.9721.4021.2321.38C H3C30.3331.6131.2531.3431.5031.15C34.2535.0034.3334.3535.0535.36chloroform CH77.3679.1979.1677.7979.1779.44cyclohexane CH226.9427.5126.3327.2327.6327.961,2-dichloroethane CH243.5045.2545.0243.5945.5445.11 dichloromethane CH253.5254.9554.8453.4655.3254.78diethyl ether CH315.2015.7815.1215.4615.6315.4614.77 CH265.9166.1262.0565.9466.3266.8866.42 diglyme CH359.0158.7757.9858.6658.9059.0658.67 CH270.5171.0369.5470.8770.9971.3370.05CH271.9072.6371.2572.3572.6372.9271.63 1,2-dimethoxyethane CH359.0858.4558.0158.6858.8959.0658.67 CH271.8472.4717.0772.2172.4772.7271.49 dimethylacetamide CH321.5321.5121.2921.1621.7621.3221.09 CO171.07170.61169.54169.95171.31173.32174.57NCH335.2834.8937.3834.6735.1735.5035.03NCH338.1337.9234.4237.0338.2638.4338.76 dimethylformamide CH162.62162.79162.29162.13163.31164.73165.53 CH336.5036.1535.7335.2536.5736.8937.54CH331.4531.0330.7330.7231.3231.6132.03 dimethyl sulfoxide CH340.7641.2340.4540.0341.3140.4539.39 dioxane CH267.1467.6066.3667.1667.7268.1167.19 ethanol CH318.4118.8918.5118.7218.8018.4017.47 CH258.2857.7256.0757.8657.9658.2658.05 ethyl acetate C H3CO21.0420.8320.6820.5621.1620.8821.15 CO171.36170.96170.31170.44171.68172.89175.26CH260.4960.5659.7460.2160.9861.5062.32CH314.1914.5014.4014.1914.5414.4913.92 ethyl methyl ketone C H3CO29.4929.3029.2628.5629.6029.3929.49 CO209.56208.30208.72206.55209.88212.16218.43C H2CH336.8936.7535.8336.3637.0937.3437.27CH2C H37.868.037.617.918.148.097.87 ethylene glycol CH263.7964.2662.7664.3464.2264.3063.17“grease”CH229.7630.7329.2030.2130.8631.29n-hexane CH314.1414.3413.8814.3214.4314.45CH2(2)22.7023.2822.0523.0423.4023.68CH2(3)31.6432.3030.9531.9632.3632.73HMPA b CH336.8737.0436.4236.8837.1037.0036.46 methanol CH350.4149.7748.5949.9749.9049.8649.50c nitromethane CH362.5063.2163.2861.1663.6663.0863.22 n-pentane CH314.0814.2913.2814.2514.3714.39CH2(2)22.3822.9821.7022.7223.0823.38CH2(3)34.1634.8333.4834.4534.8935.302-propanol CH325.1425.6725.4325.1825.5525.2724.38 CH64.5063.8564.9264.2364.3064.7164.88 pyridine CH(2)149.90150.67149.58150.27150.76150.07149.18 CH(3)123.75124.57123.84123.58127.76125.53125.12CH(4)135.96136.56136.05135.28136.89138.35138.27 silicone grease CH3 1.04 1.40 1.38 2.10 tetrahydrofuran CH225.6226.1525.1425.7226.2726.4825.67 CH2O67.9768.0767.0367.8068.3368.8368.68 toluene CH321.4621.4620.9921.1021.5021.50C(i)137.89138.48137.35137.91138.90138.85CH(o)129.07129.76128.88129.33129.94129.91CH(m)128.26129.03128.18128.56129.23129.20CH(p)125.33126.12125.29125.68126.28126.29triethylamine CH311.6112.4911.7412.3512.3811.099.07 CH246.2547.0745.7446.7747.1046.9647.19 a See footnotes for Table1.b2J PC)3Hz.c Reference material;see text.。

D-3-羟丁酸测定试剂盒(β-羟丁酸脱氢酶法)产品技术要求首医

D-3-羟丁酸测定试剂盒（β-羟丁酸脱氢酶法）适用范围：本试剂用于体外定量测定人血清中的D-3-羟丁酸的含量。

1.1产品规格1.2产品组成试剂1：Tris缓冲液100mmol/L，D-3-羟丁酸脱氢酶2KU/L。

试剂2：NAD+ 2.5mmol/L，草酸盐20mmol/L。

2.1 外观试剂1为无色透明溶液，试剂2为无色或略带黄色透明溶液；试剂盒各组分齐全、完整，液体无渗漏，包装标签文字符号清晰牢固不易脱落，外包装完整无破损。

2.2 装量液体试剂的净含量应不少于标示量。

2.3 试剂空白2.3.1试剂空白吸光度在340nm波长、1cm光径条件下，试剂空白吸光度应不大于0.30。

2.3.2 试剂空白吸光度变化率在340nm波长、1cm光径条件下，用生理盐水作为样品加入试剂测试时，试剂空白波光度变化率（ΔA/min）应不大于 0.005/min。

2.4分析灵敏度测定1.0mmol/L D-3-羟丁酸时，吸光度变化率在（0.02±0.01）/min范围内。

2.5 准确度采用比对试验，相关系数r≥0.98；[0.01，0.1]mmol/L区间内，线性绝对偏差不超过±0.01mmol/L；(0.1，4.5]mmol/L区间内，线性相对偏差不超过±10% 。

2.6 精密度2.6.1 重复性用血清样品或质控样品重复测试所得的变异系数（CV）应不大于10.0%。

2.6.2 批间差试剂（盒）批间相对极差应不大于10.0%。

2.7 线性区间试剂线性在[0.01，4.5]mmol/L区间内：a) 线性相关系数|r|应不小于0.990；b) [0.01，0.1]mmol/L区间内，线性绝对偏差应不超过±0.01mmol/L；（0.1，4.5]mmol/L区间内，线性相对偏差应不超过±10%。

2.8稳定性原包装试剂2～8℃避光保存有效期12个月，到效期后的样品检测试剂空白、分析灵敏度、准确度、重复性、线性区间应符合2.3、2.4、2.5、2.6.1、2.7的要求。

组蛋白去甲基化酶LSD1的结构和功能研究进展_阮建彬

[ 16]
1 组蛋白去甲基化酶的发现
很多组蛋白化学修饰都是可逆的 , 例如组蛋白的乙酰化、磷酸化和泛素化分别受到乙酰基转移酶和去乙酰化酶、激酶和磷酸脂酶、泛素化连接酶和去泛素化酶的动态调控 , 相应地改变着染色体的状态 , 对快速改变的基因转录过程迅速作出响应
[ 18 ～ 20]
. 但
Abstract : T he discovery of histo ne dem ethylase LSD1 is an impo rtant prog ress in t he field of epigene tics , indicati ng t hat hist one ly sine methy lation is a reve rsible and dynamic pro cess like ot her covalent histo ne mo difications such as acety lation , phosphory lation and ubiquity latio n . St ructural and functi onal research result s demonstrat e t hat LSD1 regulates t he activ ation and silencing of gene t ranscriptio n and the f unction of p53 . L SD1 play s a sig nif icant role in the development o f sev eral cancers and is a po tential target prot ein f or developing ant i-cancer drug s . Key words : LSD1 ; histone demet hy lase ; p53 ; amine o xidase ; inhibi to r

岛津色谱

在线样品提取LC-MS/MS进行蜂蜜中喹诺酮残留的检测分析简介：全球食品市场竞争越来越激烈，同时相应的花费也越来越昂贵。

分析检测需求的高通量、高灵敏度和好的重现性也在日益增长。

于是，一种新的检测方法应运而生，它可以替代传统的、耗时的固相萃取（SPE）样品提取法，这就是将自动的在线提取与串联质谱进行结合。

喹诺酮，包括氟喹诺酮，是一类合成性抗菌素，广泛用于多种感染性疾病的防治中，尤其是畜禽饲养业和水产养殖业，从而导致食品中此类药物的残留。

对此，欧盟法规规定一些化学物质在动物肉类加工制作的食品中的最低允许限量（MRPL），但是针对蜂蜜中这些化学物质的残留问题仍然是一个空白。

此外，这些化学物质的存在暗示了食品生产业中所存在不安全隐患，对蜂产品工业来说也是一个缺陷。

由于蜂蜜产品本身基质的复杂性，针对其中药物残留的分析方法首先要考虑如何消除或减弱复杂基质中的干扰因素。

Thermo Scientific Turbo Flow技术使得样品预处理的时间大大缩减，同时也消除了分析过程中蜂蜜类复杂样品基质所带来的干扰。

目标：开发出一种使用Turbo Flow™技术进行自动样品制备，对蜂蜜中12种氟喹诺酮和4种喹诺酮进行定量分析的灵敏的、可再现的LC-MS/MS方法。

实验：1.样品制备称取1g蜂蜜样品，加入1mL水充分混匀，经0.22μm聚醚砜膜注射过滤，过滤后的样品直接注入HPLC样品瓶。

使用标准储存溶液进行样品稀释，制备不同浓度水平的标准溶液进行检测分析。

12个样品总的制备时间是40分钟。

2.Turbo Flow™方法条件系统： Thermo Scientific Aria TLX-1 controlled by Aria™ software (图1)在线提取： Turbo Flow Cyclone 50 x 0.5 mm流动相A：水（含0.1% 甲酸）流动相B：乙腈（含0.1% 甲酸）流动相C：水（含10mM甲酸铵）流动相D：乙腈/异丙酮/丙酮（4：3：3 V/V/V）进样体积： 90µL3．HPLC方法条件分析柱： Thermo Scientific Hypersil GOLD 2.1 x 50 mm, 3 μm （40° C）溶剂A：水（0.5%甲酸）溶液B：甲醇/乙腈（0.5%甲酸，1：1 V/V）图1 Aria TLX-1 controlled by Aria™软件4. MS条件Thermo Scientific TSQ Quantum Ultra AM三重四极杆质谱仪，检测条件如下：离子源极性：阳离子模式喷雾电压： 3000V气化温度： 350°C鞘气压(N2)： 40 units辅助气压(N2)： 35 units毛细管柱温度： 325°C碰撞气体（Ar）： 1.5mTorrQ1/Q3峰分辨度： 0.7 u (单位分辨质量)扫描时间： 0.025s扫描宽度： 0.010m/z数据获取模式：选择反应监测（SRM）直接注入标准溶液进行选择反应监测（SRM）相关参数的优化。

BD OptiBuild

BD OptiBuild™Technical Data SheetBB700 Rat Anti-Mouse CD8bProduct InformationMaterial Number:742198Size:50 µgClone:H35-17.2Alternative Name:Ly-3; Lyt-3; Lymphocyte antigen 3; Ly-C; CD8b1Reactivity:Tested in Development:MouseIsotype:Rat IgG2b, κImmunogen:5-day MLR, C57BL/6 anti-BALB/cApplication:Flow cytometry(Qualiﬁed)Concentration:0.2 mg/mlEntrez Gene ID:12526Storage Buﬀer:Aqueous buﬀered solution containing ≤0.09% sodium azide. Regulatory Status:RUODescriptionThe H35-17.2 monoclonal antibody speciﬁcally binds to both alloantigeneic forms of the β chain of the CD8diﬀerentiation antigen (Ly-3 or Lyt- 3). The CD8 α and α' chains (CD8a) form heterodimers with the CD8 β chain (CD8b, Ly-3, or Lyt-3) on the surface of most thymocytes. A subpopulation of mature T lymphocytes (i.e., MHC class I-restrictedT cells, including most T suppressor/cytotoxic cells) expresses almost exclusively the CD8 αβ heterodimer (the α' chain is absent). Subsets of γδ TCR-bearing T cells, intestinal intraepithelial lymphocytes, and dendritic cells express CD8a without CD8b. It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to T lymphocytes which matured in the thymus or in an extrathymic environment that had been inﬂuenced by thymus- initiated neuroendocrine signals. CD8 is an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck (p56lck). The H35-17.2 mAb blocks T-cell-mediated cytolysis of allogeneic lymphoma cells.The antibody was conjugated to BD Horizon™ BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes. It is a polymer-based tandem dye developed exclusively by BD Biosciences. With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg, 695/40-nm ﬁlter). This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation oﬀ the 405 nm and 355 nm lasers.Preparation and StorageStore undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody waspuriﬁed from tissue culture supernatant or ascites by aﬃnity chromatography. The antibody was conjugated with BD Horizon BB700 under optimal conditions that minimize unconjugated dye and antibody.Recommended Assay ProcedureFor optimal and reproducible results, BD Horizon Brilliant Stain Buﬀer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may aﬀect data interpretation. The BD Horizon Brilliant Stain Buﬀer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buﬀer (Cat. No. 563794 or 566349).When setting up compensation, it is recommended to compare spillover values obtained from cells and BD™ CompBeads to ensure that beads will provide suﬃciently accurate spillover values.For optimal results, it is recommended to perform two washes after staining with antibodies. Cells may be prepared, stained with antibodies and washed twice with wash buﬀer per established protocols for immunoﬂuorescent staining prior to acquisition on a ﬂow cytometer. Performing fewer than the recommended wash steps may lead to increased spread of the negative population.Suggested Companion ProductsCatalog Number Name Size Clone553141Puriﬁed Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™)0.1 mg 2.4G2 554656Stain Buﬀer (FBS)500 mL554657Stain Buﬀer (BSA)500 mL563794Brilliant Stain Buﬀer100 Tests555899Lysing Buﬀer100 mLProduct Notices1.This antibody was developed for use in ﬂow cytometry.2.The production process underwent stringent testing and validation to assure that it generates a high-qualityconjugate with consistent performance and speciﬁc binding activity. However, veriﬁcation testing has not been performed on all conjugate lots.3.Researchers should determine the optimal concentration of this reagent for their individual applications.4.An isotype control should be used at the same concentration as the antibody of interest.5.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in runningwater before discarding to avoid accumulation of potentially explosive deposits in plumbing.6.For ﬂuorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at/colors.7.Please refer to /us/s/resources for technical protocols.8.BD Horizon Brilliant Stain Buﬀer is covered by one or more of the following US patents: 8,110,673; 8,158,444;8,575,303; 8,354,239.9.BD Horizon Brilliant Blue 700 is covered by one or more of the following US patents: 8,455,613 and 8,575,303.10.Cy is a trademark of GE Healthcare.ReferencesGolstein P, Goridis C, Schmitt-Verhulst AM . Lymphoid cell surface interaction structures detected using cytolysis-inhibiting monoclonal antibodies. Immunol Rev. 1982; 68:5-42. (Immunogen: Cytotoxicity, Immunoprecipitation, Inhibition). Lefrancois L. Phenotypic complexity of intraepithelial lymphocytes of the small intestine. J Immunol. 1991;147(6):1746-1751. (Biology).Ledbetter JA, Seaman WE, Tsu TT, Herzenberg LA. Lyt-2 and Lyt-3 antigens are on two diﬀerent polypeptide subunits linked by disulﬁde bonds. Relationship of subunits to T cell cytolytic activity. J Exp Med. 1981; 153:1503-1516. (Biology). Walker ID, Murray BJ, Hogarth PM, Kelso A, McKenzie IF. Comparison of thymic and peripheral T cell Ly-2/3 antigens. Eur J Immunol. 1984; 14(10):906-910. (Biology).Nakayama K, Nakayama K, Negishi I, et al. Requirement for CD8 beta chain in positive selection of CD8-lineage T cells. Science. 1994; 263(5150):1131-1133. (Biology).MacDonald HR, Schreyer M, Howe RC, Bron C. Selective expression of CD8 alpha (Ly-2) subunit on activated thymic gamma/delta cells. Eur J Immunol. 1990; 20(4):927-930. (Biology).Rocha B, Vassalli P, Guy-Grand D. The extrathymic T-cell development pathway. Immunol Today. 1992; 14(3):140-141. (Biology).Murosaki S, Yoshikai Y, Ishida A, et al. Failure of T cell receptor V beta negative selection in murine intestinal intra-epithelial lymphocytes. Int Immunol. 1991; 3(10):1005-1013. (Biology).Wang J, Klein JR. Thymus-neuroendocrine interactions in extrathymic T cell development. Science. 1994;265(5180):1860-1862. (Biology).Sydora BC, Brossay L, Hagenbaugh A, Kronenberg M, Cheroutre H. TAP-independent selection of CD8+ intestinal intraepithelial lymphocytes. J Immunol. 1996; 156(11):4209-4216. (Biology).Vremec D, Zorbas M, Scollay R, et al. The surface phenotype of dendritic cells puriﬁed from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells. J Exp Med. 1992; 176(1):47-58. (Biology).Wu L, Vremec D, Ardavin C, et al. Mouse thymus dendritic cells: kinetics of development and changes in surface markers during maturation. Eur J Immunol. 1995; 25(2):418-425. (Biology).Süss G, Shortman K. A subclass of dendritic cells kills CD4 T cells via Fas/Fas-ligand-induced apoptosis. J Exp Med. 1996; 183(4):1789-1796. (Biology).Fujiura Y, Kawaguchi M, Kondo Y, et al. Development of CD8 alpha alpha+ intestinal intraepithelial T cells in beta 2-microglobulin- and/or TAP1-deﬁcient mice. J Immunol. 1996; 156(8):2710-2715. (Biology).Bierer BE, Sleckman BP, Ratnofsky SE, Burakoﬀ SJ. The biologic roles of CD2, CD4, and CD8 in T-cell activation. Annu Rev Immunol. 1989; 7:579-599. (Biology).Janeway CA Jr. The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation. Annu Rev Immunol. 1992; 10:645-674. (Biology).Zamoyska R. The CD8 coreceptor revisited: one chain good, two chains better. Immunity. 1994; 1(4):243-246. (Biology). LeFrancois L. Extrathymic diﬀerentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire. Immunol Today. 1991; 12(12):436-438. (Biology).O'Rourke AM, Mescher MF. The roles of CD8 in cytotoxic T lymphocyte function. Immunol Today. 1993; 14(4):183-188. (Biology).Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA. T cell subsets deﬁned by expression of Lyt-1,2,3 and Thy-1 antigens.Two-parameter immunoﬂuorescence and cytotoxicity analysis with monoclonal antibodies modiﬁes current views. J ExpMed. 1980; 152(2):280-295. (Biology).BD BiosciencesUnited States Canada Europe Japan Asia Paciﬁc Latin America/Caribbearn877.232.8995888.268.543032.53.720.5500120.8555.9065.6861.06330800.771.7157For country contact information, visit /contactConditions: The information disclosed herein is not to be construed as a recommendation to use the above product in violation of any patents. BD Biosciences will not be held responsible for a patent infringement or other v ©2020 BD. All rights reserved. Unless otherwise noted, BD, the BD Logo and all other trademarks are the property of Becton, Dickinson and Company or its aﬃliates.。

HP 打印机说明书

DimensionsW x D x H . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .660 mm x 650 mm x 725 mm ( 26 in x 26 in x 29 in)Weight . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .103 kg (227 lb)Operating EnvironmentTemperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15 – 35 °C Humidity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 – 80%, non-condensing Altitude . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3048 m (10,000 ft) max Heat Generation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4092 BTU/h Power Consumption . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1200 VA max Power Input Requirements . . . . . . . . . . . . . . . . . . . .100 – 240 V ~, 50 – 60 Hz User Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Integrated LCD touchscreen Adapter/Ports■■Ethernet on RJ45 port ■■RS232 on DB9 port■■External DVI-I connectors (with VGA)■■ 6 External USB portsLiquid Waste Handling■■ 2 external waste bottles (5 L each)■■Can be connected to a laboratory drain Printing Options■■On-screen result review ■■Electronic printing to PDF ■■External printer (available)Required Maintenance■■Empty waste bottles (as needed)■■Clean surfaces, touchscreen, and racks (as needed)■■Perform the Clean System procedure (2 minute automated process, every 30,000 tests)LIS Speciﬁ cations■■Bi-directional capability with broadcast download of test orders ■■Option to add patient demographics to printed results ■■LIS result output with option to include comment record ■■Chromatogram PDF can be sent automatically to a network folderD-100™ HbA 1c Elution Buffer A Volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2600 mL Number of Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . > 1100 tests per bottle Storage Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15 – 35°C Onboard Capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 bottles D-100™ HbA 1c Elution Buffer B Volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1400 mL Number of Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . > 3000 tests per bottle Storage Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15 – 35°C Onboard Capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 bottles D-100™ Wash Solution Volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3300 mL Number of Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . > 1100 tests per bottle Storage Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15 – 35°C Onboard Capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 bottles D-100™ HbA 1c Analytical CartridgeNumber of Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10,000Storage Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2 – 8°C Onboard Capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 cartridge D-100™ HbA 1c Calibrator Pack Calibration Frequency . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .once per cartridge Storage Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2 – 8°C D-100™ Preﬁ ltersNumber of Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2,000Storage Requirements . . . . . . . . . . . . . . . . . . . . . .2 – 8°C, 120 days at 15 – 35°C Onboard Capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 preﬁ lterGeneral Specifi cationsConsumable SpecificationsHbA1c Testing for DiabetesD-100™ Hemoglobin Testing SystemFor further information, please contact the Bio-Rad office nearest you or visit our website at /diagnosticsClinical Diagnostics Group Web site /diagnostics USA 180****6723Australia 61 2 9914 2800 Austria 43 1 877 8901 Belgium 32 03 710 53 00Brazil 55 31 3689 6600 Canada 151****4372China 86 21 61698500 Czech Republic 420 241 430 532 Denmark 45 4452 1000 Finland 358 9 804 22 00 France 33 1 47 95 60 00 Germany 49 0 89 318 840 Greece 30 210 7774396 Hong Kong 852 2789 3300 Hungary 36 1 459 6100 India 180****1224 Israel 972 3 9636050 Italy 39 02 216091 Japan 81 3 6361 7070 Korea 82 2 3473 4460 Mexico 52 55 5488 7670 The Netherlands 31 318 540666 New Zealand 64 9 415 2280 Norway 47 23 38 41 30 Poland 48 22 3319999 Portugal 351 21 472-7700 Russia 7 495 721 1404 Singapore 65 6415 3170 South Africa 27 11 442 85 08 Spain 34 91 590 5200 Sweden 46 8 555 127 00 Switzerland 41 0 26 674 55 05 06 Taiwan 886 2 2578 7189Thailand 662 651 8311 United Kingdom 44 0 20 8328 2000Bio-RadLaboratories, Inc.© 2016 Bio-Rad Laboratories, Inc. Printed in USA DG16-0012 A-318 Rev. 07/2016Printed on recycled paperwith soy-based inksD-100™ Hemoglobin Testing SystemSample Handling SpecificationsSample Throughput . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 tests/hour Time to First Result . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2 minutes, 15 seconds Sample Analysis Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45 seconds Sample Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .None required Sample Capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .100 samples, continuous loading Stat Area Sample Capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3 samples (primary tube or microvial) Minimum Sample Volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1 mL (13 x 75 mm primary tube) Specimen Type . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Whole blood, capillary** Whole Blood Sample Aspiration Volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9 μL Prediluted Sample Aspiration Volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .495 μL Acceptable Sample Tube Types■■K2-EDTA■■K3-EDTA■■Potassium Oxalate/Sodium Fluoride■■Sodium Citrate ■■Sodium Heparin ■■Lithium HeparinSupported Barcode Symbologies■■Code 39■■Code 128■■Codabar■■Interleaved 2 of 5■■EAN■■UPCPerformance SpecificationsTotal Precision* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .≤1 .7% CV (NGSP units) Accuracy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Total Error <6% Linear Range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3 .5% – 20% HbA1c Minimum QC Frequency . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Once per day Sample Concentration Range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .50,000 – 350,000 mAU HbA1c Units Reported . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .IFCC and NGSP Hemoglobin Interferences■■No interference from hemoglobins S, C, D, or E ■■No interference from fetal hemoglobin up to 30%■■No interference from labile HbA1c■■No interference from carbamylated hemoglobin* Total precision is based on a 3-instrument, 60-day precision study using 3 lots of reagents to generate a total of 720 data points per sample . It includes the following sources of variation: within-run (repeatability), between-run, between-day, between-instrument, and between-lot .** Not available in US for the capillary collection kit .。

一氧化氮(NO)含量检测试剂盒.96S

一氧化氮（NO）含量检测试剂盒说明书(货号：NM-W-0132 微量法100T/96S)一、产品简介：一氧化氮（Nitric Oxide，NO）是一种气态分子自由基，在生物体内作为重要的信使分子和效应分子，参与调节血管形成、神经传递、免疫调控及肿瘤生长等多种生理及病理过程。

此外，NO是植物的主要生物活性分子，在植物的生长发育和对胁迫的反应过程中参与了多种生理活动，如种子萌发、叶扩展、根生长、花器官发生、气孔运动的调节以及植物的抗逆反应等。

NO在体内或水溶液中极易氧化生成NO2-。

在酸性条件下，NO2-与重氮盐磺酸胺生成重氮化合物，进一步与萘基乙烯基二胺偶合，产物在550nm处有特征吸收峰，测定其吸光值，可以计算NO含量。

酶标仪、96孔板、台式离心机、恒温水浴锅/培养箱、超声细胞破碎仪、研钵/匀浆器、天平、可调式移液器、冰和蒸馏水。

四、操作步骤：建议正式实验前选取2个样本做预测定，了解本批样品情况，熟悉实验流程，避免实验样本和试剂浪费！1、预实验样本制备℃ 组织样本：称取0.2g样本，加入1mL提取液进行冰浴匀浆后，10000g，4℃离心15min，取上清置于冰上待测。

【注】：若增加样本量，可按照组织质量(g)：提取液体积(mL)为2~5：10的比例进行提取。

℃ 细菌/细胞样本：建议1000万细菌或细胞加入1mL提取液，超声波破碎细菌或细胞（冰浴，功率200W，超声3s，间隔7s，总时间5min），10000g，4℃离心15min，取上清置于冰上待测。

【注】：若增加样本量，可按照细菌/细胞数量（104）：提取液（mL）为1000~2000：1的比例进行提取。

℃ 血清（浆）等液体样本：直接测定。

若液体有浑浊则离心取上清测定。

2、标准溶液的配制：把10μmol/mL标准品母液用蒸馏水稀释成1.25μmol/mL的标准溶3、预实验上机操作：℃ 酶标仪预热30min以上，调节波长至550nm。

4、正式实验：℃ 若预实验∆A 测定小于0.005，可以增加样本量后再进行测定；如果∆A测定大于1.5，建议将样本上清用稀释液适当稀释后再进行测定，并将改变后的W和D代入公式计算；℃ 确定好最适实验条件后，正式实验样本制备同预实验样本制备；℃ 正式实验按照预实验操作步骤进行（标准管和空白管预实验已做，正式实验可选做）。

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C48 0.1uF
C49 0.1uF
项目名称: 文件编号: 制图: 审核: 开始时间:
嵌入式LM3S9B96学习板
项目型号: 版本号:
2010-06-15
1 C54 18pF D GND
2 C55 C52
1 18pF 18pF
2
LSD/EDC-SCH10034
V1.0
25MHZ
CVDD LDO VDD25 VDD25 7 38 88
+5V 1 3 C59 4.7uF
U15 VIN EN GND VOUT BYP
倪孟余
制图日期: 审核日期: 完成时间: 修改记录
受控类型: 纸张型号: B
4 1 共__页第__页 D
C57 0.01uF
C58 2.2uF
GND
GND
SPX5205-1.2V GND GND
2
Main Board
1 2 3
R65 1.5K U17 1 3 2
注意封装SOT23和引脚间的关系
PA3 A
JP15
Card_CS MOSI MCLK MISO +3.3V C70 0.1uF +3.3V
CATHODE NC ANODE LM4040A30IDBZR A
PA5/SSI0TX JP17 JP19 PA2/SSI0CLK PA4/SSI0RX JP20
A
U14 PA0/U0RX PA1/U0TX PA2/SSI0CLK PA3/SSI0FSS PA4/SSI0RX PA5/SSI0TX PA6/USB0EPEN PA7/USB0PFLT PC0/TCK/SWCLK PC1/TMS/SWDIO PC2/TDI PC3/TDO/SWO PC4/EPI0S02 PC5/EPI0S03 PC6/EPI0S04 PC7/EPI0S05 PE0/EPI0S08 PE1/EPI0S09 PE2/EPI0S24 PE3/EPI0S25 PE4/ADC3 PE5/ADC2 PE6/ADC1 PE7/ADC0 PG0/EPI0S13 PG1/EPI0S14 PG7/EPI0S31 PJ0/EPI0S16 PJ1/EPI0S17 PJ2/EPI0S18 PJ3 PJ4 PJ5 PJ6 PJ7 RST_n XTLN XTLP OSC0 OSC1 NC ERBIAS VDDA GNDA GND GND GND GND GND GND GND USB0DP USB0DM VDD VDD VDD VDD VDD VDD VDD VDD PB0/USB0ID PB1/USB0VBUS PB2/CCP0 PB3 PB4/EPI0S23 PB5/EPI0S22 PB6/AVREF PB7/NMI PD0 PD1 PD2/EPI0S20 PD3/EPI0S21 PD4 PD5 PD6 PD7 PF0 PF1 PF2/LED1 PF3/LED0 PF4/EPI0S12 PF5/EPI0S15 PH0/EPI0S06 PH1/EPI0S07 PH2/EPI0S01 PH3/EPI0S00 PH4/EPI0S10 PH5/EPI0S11 PH6/EPI0S26 PH7/EPI0S27 MDIO TXOP TXON RXIP RXIN USB0RBIAS 66 67 72 65 92 91 90 89 10 11 12 13 97 98 99 100 47 61 60 59 42 41 86 85 84 83 76 63 62 15 58 43 46 40 37 73 71 70 8 20 32 44 56 68 81 93 R56 PB0/USBID PB1/USBVBUS PB2/I2C0SCL PB3/I2C0SDA PB4/ADC10/EPI23 PB5/EPI22 PB6/TXSCK/AVREF PB7/NMI PD0/I2SRXSCK PD1/I2SRXWS PD2/EPI20 PD3/EPI21 PD4/I2SRXSD PD5/I2SRXMCLK PD6 PD7 PF0 PF1/TXMCLK PF2/LED1 PF3/LED0 PF4/EPI12 PF5/EPI15 PH0/EPI06 PH1/EPI07 PH2/EPI01 PH3/EPI00 PH4/EPI10 PH5/EPI11 PH6/EPI26 PH7/EPI27 ETH_MDIO ETH_TXOP ETH_TXON ETH_RXIP ETH_RXIN 9.1K GND USBDP USBDM
K
GND 10 11 12 13 9 PF3/LED0
GND JP21 LED R70 330 A K
PWR2.5
JP22 P3V JP23 M3V
TP3 3.3V
+3.3V M+3.3V
Header 17X2 GND
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 31 32
Header 16X2 GND
GND
Header 25X2 B
PF2/LED1 JP11 PF3/LED0 JP12
LED1 LED0
ETH_LED1 ETH_LED0 GND Eth_+3.3V
GND P6 PE2/EPI24 PE7/ADC0 PE3/EPI25 PE6/ADC1 PD4/I2SRXSD PD1/I2SRXWS PH7/EPI27 PD0/I2SRXSCK PD3/EPI21 PD6 PD7 PD2/EPI20 PD5/I2SRXMCLK PB5/EPI22 PH6/EPI26 PB7/NMI 1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 BLON TOUCH_XN TOUCH_YP TOUCH_YN TOUCH_XP LCD_D4 LCD_D1 LCD_DC LCD_D0 LCD_D3 LCD_D6 LCD_D7 M+3.3V LCD_D2 LCD_D5 R50 LCD_RDn 10K LCD_WRn LCD_RSTn +5V P7 R47 0 1 +3.3V 3 LCD_D0 L32_DB0 5 LCD_D2 L32_DB2 7 LCD_D4 L32_DB4 9 LCD_D6 L32_DB6 11 L32_DB8 13 L32_DB10 15 L32_DB12 17 L32_DB14 19 R49 L32_CS 21 LCD_RDn L32_RD 10K 23 LCD_RSTn RESET 25 TOUCH_XPL32_TOUCH_XP GND 27 TOUCH_YPL32_TOUCH_YP 29 +5V R48 0 ILED+ L32_DB1 LCD_D1 L32_DB3 LCD_D3 L32_DB5 LCD_D5 L32_DB7 LCD_D7 L32_DB9 L32_DB11 L32_DB13 L32_DB15 LCD_DC L32_RS LCD_WRn L32_WR BLON L32_BL L32_TOUCH_XN TOUCH_XN L32_TOUCH_YN TOUCH_YN A
4
5 +3.3V
6
Reset
SW1 +3.3V SDCARD-2908 C69 0.1uF GND R68 100 C67 0.1uF
R64 10K RESET_SWn
DBG+5V +3.3V JP13 ICDI R67 330 A A K +VBUS JP14 OTG LED3 Red JP18 J1 1 3 2 GND A GND LED4 Green +5V SW2 C73 GND 10uF C74 0.1uF R73 10K TP4 5V 1 3 U18 IN EN GND OUT NR 5 C71 4 10uF C75 10pF 0.1uF C72 JP16 EXT C68 0.1uF GND C66 2.2uF +5V VREF_3.0V PB6/TXSCK/AVREF VREF 3.0V
1
2
3
4
5
6
P5 GND PG0/EPI13 PG1/EPI14 PH7/EPI27 PJ0/EPI16 PD3/EPI21 PD2/EPI20 PJ6/EPI30 PJ5/EPI29 PJ4/EPI28 PJ3/EPI19 PE3/EPI25 PE2/EPI24 PB4/ADC10/EPI23 PB5/EPI22 PJ1/EPI17 PH0/EPI06 PH1/EPI07 PH2/EPI01 PH3/EPI00 PH4/EPI10 1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 41 43 45 47 49 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42 44 46 48 50 GND PC4/EPI02 PC5/EPI03 PC6/EPI04 PC7/EPI05 PB2/I2C0SCL PB3/I2C0SDA +3.3V +5V PG7/EPI31 PJ2/EPI18 PF5/EPI15 PF4/EPI12 PE1/EPI09 PE0/EPI08 PH5/EPI11 PH6/EPI26 GND 26 PA0/U0RX PA1/U0TX 27 28 PA2/SSI0CLK PA3 29 30 PA4/SSI0RX 31 PA5/SSI0TX PA6/USBEPE/CAN0RX 34 PA7/USBPFLT/CAN0TX35 INT_TCK TMS/SWDIO PC2/TDI PC3/TDO PC4/EPI02 PC5/EPI03 PC6/EPI04 PC7/EPI05 PE0/EPI08 PE1/EPI09 PE2/EPI24 PE3/EPI25 PE4/I2STXWS PE5/I2STXSD PE6/ADC1 PE7/ADC0 PG0/EPI13 PG1/EPI14 PG7/EPI31 PJ0/EPI16 PJ1/EPI17 PJ2/EPI18 PJ3/EPI19 PJ4/EPI28 PJ5/EPI29 PJ6/EPI30 PJ7 C MCURSTn 80 79 78 77 25 24 23 22 74 75 95 96 6 5 2 1 19 18 36 14 87 39 50 52 53 54 55 64 17 16 48 49 51 R60 GND M+3.3V C51 0.1uF Y2 Y3 GND C53 16MHZ 18pF GND GND LM3S9B96 12.4K 33 3 4 9 21 45 57 69 82 94