Chapter 7 Compressible Flow Some Preliminary Aspe
Q Sepharose Fast Flow 产品说明书
Q1126pis Rev 01/221Product InformationQ Sepharose ® Fast FlowQ1126Product DescriptionQ Sepharose ® Fast Flow is an ion exchangechromatography resin with a quaternary amine (Q) functional group [-CH 2-N +(CH 3)3] attached to Sepharose ® Fast Flow. The Q group serves as astrong anion exchanger, which is completely ionized over a broad pH range. The terms “s trong" and"weak" in ion exchange chromatography refer to the extent of ionization with pH, and not to the binding strength of the functional group to the target species. The parent Sepharose ® Fast Flow is a cross-linked derivative of Sepharose ®. The particle size range is 45-165 µm. The average bead diameter is ~90 µm. The counterion in the product is sulfate (SO 4-2). Recommended cation buffers to use with Q Sepharose ® Fast Flow include alkylamines,ammonium, ethylenediamine, imidazole, pyridine, or Tris. In terms of pH, it is suggested to operate within 0.5 pH unit of the buffer's pK a . With proteins, it is suggested to operate at least 1 pH unit above the pI of the protein, to facilitate binding. Oxidizing agents, and anionic detergents and buffers, should not be used with Q Sepharose ® Fast Flow. Likewise,extended exposure of Q1126 to pH < 4 should be avoided. Several publications 1,2 and dissertations 3-5 cite use of product Q1126 in their research.ReagentQ Sepharose ® Fast Flow is offered as a suspension in 20% ethanol.Approximate Exclusion Limit: average molecular mass of ~4 × 106 DaltonsIonic Capacity: 0.18-0.24 mmol Cl -/mL gel Binding Capacity: ~42 mg BSA per mL gel pH Stability: 2-12Working temperature: 4-40 °CPrecautions and DisclaimerFor R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices. General Resin Preparation Procedure1. Allow the ion exchange medium and ~10 columnvolumes (CV) of buffer to equilibrate to thetemperature chosen for the chromatographic run. 2. Mix the pre-swollen suspension with startingbuffer to form a moderately thick slurry, which consists of ~75% settled gel and 25% liquid. 3. Degas the gel under vacuum at the temperatureof column operation.4. Mount the column vertically on a suitable stand,out of the way of direct sunlight or drafts, which may cause temperature fluctuations.5. Pour a small amount of buffer into the emptycolumn. Allow the buffer to flow through spaces to eliminate air pockets.6. Pour the suspension of ion exchange mediumprepared in Step 3 into the column by allowing it to flow gently down the side of the tube, to avoid bubble formation.7. For consistent flow rates and reproducibleseparations, connect a pump to the column. 8. Fill the remainder of the column to the top withbuffer. Allow ~5 CV of buffer to drain through the bed at a flow rate at least 133% of the flow rate to be used in the procedure. The bed height should have settled to a constant height.9. Using a syringe or similar instrument, apply thesample dissolved in starting buffer to the column. For isocratic separations, the sample volumeshould range from 1-5% of the column volume. If the chromatographic run involves elution with a gradient, the applied sample mass is of much greater importance than the sample volume, and the sample should be applied in a low ionicstrength medium. Ion exchange is used both to concentrate and to fractionate the sample. 10. Elution:• If only unwanted substances in the sample areadsorbed, or if sample components aredifferentially retarded under isocratic conditions, the starting buffer can also be used as the eluent.The life science business of Merck operates as MilliporeSigma in the U.S. and Canada.Merck and Sigma-Aldrich are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates. All other trademarks are the property of their respective owners. Detailed information on trademarks is available via publicly accessible resources.© 2022 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved. Q1126pis Rev 01/22 JJJ,MAM,GCY2•Normally, however, separation and elution are achieved by selectively decreasing the affinity of the molecules for the charged groups on the resin by changing the pH and/or ionic strength of the eluent. This procedure is termed gradient elution. 11. Regeneration: •Either (a) washing the column with a high ionic strength salt solution, such as 1 M NaCl, or (b) changing the pH to the tolerable low and high pH extremes, is usually sufficient to remove reversibly bound material.• When needed, lipids and precipitated proteins canbe removed by washing with 1 CV of 1-2 M NaCl, followed by 1 CV of 0.1 M NaOH in 0.5 M NaCl. • Rinse with several CV of water. Thenre-equilibrate the resin with starting buffer.• If base such as NaOH was used, adjust the pH ofthe resin to neutral before storing or using.12. Storage: Q Sepharose ® Fast Flow may be storedat 2-8 °C in water with 20% ethanol added as an antibacterial agent.General NotesCation versus Anion Exchanger• If sample components are most stable below their pI values, a cation exchanger should be used. • If sample components are most stable above their pI values, an anion exchanger should be used. •If stability is good over a wide pH range on both sides of the pI, either or both types of ion exchanger may be used.Strong versus Weak Ion Exchanger•Most proteins have pI values within the range 5.5-7.5, and can thus be separated on both strong and weak ion exchangers.•When maximum resolution occurs at an extreme pH and the molecules of interest are stable at that pH, a strong ion exchanger should be used. Choice of Buffer, pH, and Ionic Strength• The highest ionic strength which permits binding should normally be used.•The required buffer concentration varies fromsubstance to substance. Usually, an ionic strength of at least 10 mM is required to ensure adequate buffering capacity.•As salts (such as buffers) help to stabilize proteins in solution, their concentration should be highenough to prevent denaturation and precipitation.References1. López, G. et al ., Eukaryot. Cell , 14(6), 564-577(2015).2. Bhargava, V. et al ., Dev. Cell., 52(1), 38-52.e10(2020).3. Fu , Yinan, “Structure and dynamics ofPseudomonas aeruginosa ICP”. University ofGlasgow, Ph.D. dissertation, p. 126 (April 2009). 4. Redmond, Miranda , “The Role of N-TerminalAcidic Inserts on the Dynamics of the Tau Protein ”. University of Vermont, Ph.D. dissertation, p. 22 (May 2017).5. Taylor-Whiteley, Teresa Rachel , “RecapitulatingParkinson’s disease pathology in athree-dimensional neural cell culture mode ”. Sheffield Hallam University, Ph.D. dissertation, p. 58 (September 2019).NoticeWe provide information and advice to our customers on application technologies and regulatory matters to the best of our knowledge and ability, but without obligation or liability. Existing laws and regulations are to be observed in all cases by our customers. This also applies in respect to any rights of third parties. Our information and advice do not relieve ourcustomers of their own responsibility for checking the suitability of our products for the envisaged purpose. The information in this document is subject to change without notice and should not be construed as acommitment by the manufacturing or selling entity, or an affiliate. We assume no responsibility for any errors that may appear in this document.Technical AssistanceVisit the tech service page at /techservice .Standard WarrantyThe applicable warranty for the products listed in this publication may be found at /terms .Contact InformationFor the location of the office nearest you, go to /offices .。
FundamentalsofAerodynamics
Fundamentals of Aerodynamics
Fourth Edition
John D. Anderson, Jr.
Curator of Aerodynamics National Air and Space Museum Smithsonian Institution and Professor Emeritus University of Maryland
பைடு நூலகம்
Contents
xiii
Chapter 4 Incompressible Flow over Airfoils 295 4.1 4.2 4.3 4.4 Introduction 297 Airfoil Nomenclature 300 Airfoil Characteristics 302 Philosophy of Theoretical Solutions for Low-Speed Flow over Airfoils: The Vortex Sheet 307 4.5 The Kutta Condition 312
Inventarisiert unter
TECHWISCHE UNIVERSITAT DARMSTADT
Fachgebiot Konstruktiver Leichtbau und Bauwelsen Prof. Dr.-lng. Helmut Schurmann S4287 Darmstadt, PetersenstraBe 30
187
3.19
3.20 3.21 3.22
190
Incompressible Flow in a Duct: The Venturi and Low-Speed Wind Tunnel 197 Pitot Tube: Measurement of Airspeed 210 Pressure Coefficient 219 Condition on Velocity for Incompressible Flow 221 Governing Equation for Irrotational, Incompressible Flow: Laplace's Equation 222 3.7.1 -. Infinity Boundary Conditions 225 3.7.2 Wall Boundary Conditions 225 Interim Summary 226 Uniform Flow::Our First Elementary Flow 227 Source Flow: O u r Second Elementary Flow 229 : ...; .•,..= :. ....• Combination of a Uniform Flow with a Source and Sink 233 Doublet Flow: Our Third Elementary Flow 237 Nonlifting Flow over a Circular Cylinder 239 Vortex Flow: Our Fourth Elementary Flow 245 Lifting Flow over a Cylinder 249 The Kutta-Joukowski Theorem and the Generation of Lift 262 Nonlifting Flows over Arbitrary Bodies: The Numerical Source Panel Method 264 Applied Aerodynamics: The Flow over a Circular Cylinder—The Real Case 274 Historical Note: Bernoulli and Euler—The Origins of Theoretical Fluid Dynamics 282 Historical Note: d'Alembert and His Paradox 287 Summary 288 Problems 291
进出口边界条件各种说法
问：用了很长时间的fluent，但一直没有把压力出入口边界条件弄明白。
请大侠给予正确指导... 有的文档说亚声速流下initial是0或者不填，而有的出版物则把total和initial设置成几乎想等的值，或者差值为大气压，很困惑！比如说在一个喷射（亚声速流）流场中，实际条件为喷嘴入口压力40MPa，出口压力20MPa，即流场内围压20MPa，这时，在压力入口边界条件的总压、初始表压以及压力出口的表压分别应该设置多少？如果是超声速流，又有什么区别？还有，operating condition下的operating pressure是否设置成0或者大气压有什么说法吗？A：有的出版物则把total和initial设置成几乎想等的值。
我在使用时一般也是采用这样的方法，严格来讲是有公式来计算的。
但是这个值一般只是用于初始化，对结果影响不大，所以简单来讲就设置成和出口的一样。
这个值对流场的初始化有一定的影响，设置成0也不是不可以，但会增加迭代步数。
对于喷射而言，建议lz将operating condition下的operating pressure设置为0 ，即是绝对压力。
二最近用Fluent做模拟的时候一直在使用压力出口边界，对其中出口温度、组分浓度等值的设置不是很明白，就仔细看了下Fluent User Guide，对压力出口边界描述如下：Pressure outlet boundary conditions require the specification of a static (gauge) pressure at the outlet boundary........All other flow quantities are extrapolated from the interior。
因此，压力出口边界可以这样表述，即，给定出口压力，对流动中的其他物理量均有流场内部值差值得到。
那边界条件面板中设定的温度（等）值有什么用呢？是出现回流时的回流值。
恋爱中的闪躲技巧小说
恋爱中的闪躲技巧小说Chapter 1: Love in the AirSophie sat under a tall oak tree in the park, lost in her thoughts, her heart fluttering with anticipation. She had fallen madly in love with Jack, an incredibly charming and handsome young man she had met at a friend's birthday party a few weeks ago. They had been chatting online and had agreed to meet today for a picnic. Sophie was excited, but also a little nervous. She wanted to make a good impression and hoped their connection would continue to grow stronger.Chapter 2: The Unexpected EncounterSophie spread out the picnic blanket and carefully arranged the delicious sandwiches and fresh fruits she had prepared. Just as she was putting the finishing touches, a sudden gust of wind blew her carefully coiffed hair into a tangled mess. Frantically, she searched in her bag for a hair tie, but to no avail. Just when she thought all hope was lost, she spotted a familiar face in the distance. It was Lily, her childhood friend.Chapter 3: The Perfect DisguiseSophie's heart skipped a beat as she noticed Lily approaching. Desperately seeking help, she hatched a plan. "Lily, it's you!" she exclaimed, putting on her most surprised face. "What a coincidence! I was just about to have a picnic all by myself. Care to join?"Lily, delighted to meet her old friend, gladly accepted the invitation. Sophie skillfully maneuvered the conversation towards Jack, seizing the opportunity to gather any juicy details about him. She discovered that he was a music lover, had a soft spot for romantic comedies, and had a passion for cooking. Armed with this information, Sophie felt a renewed sense of confidence.Chapter 4: The Serendipitous EncounterAs they chatted and laughed, Sophie's eyes wandered to the path leading to the picnic spot. Suddenly, she noticed a familiar figure in the distance. It was Jack! Panic flooded through her veins, but she suppressed the urge to let it consume her.Sophie skillfully guided Lily's attention away from the path by pointing out a beautiful bird perched on a nearby tree. In that moment, she mustered all her courage and discreetly gestured for Jack to stay hidden. With a hint of relief, he complied, concealing himself behind a bush.As the sun began to set, Sophie and Lily said their goodbyes. Sophie thanked Lily for keeping her company and promised to meet up again soon. Once Lily was out of sight, Sophie quickly made her way to Jack's hiding spot.Breathing a sigh of relief, they embraced, their hearts beating in synchrony. Jack smiled, his eyes twinkling with adoration. In that moment, Sophie knew they had successfully dodged the awkwardness of their unexpected encounter.From that day forward, Sophie and Jack's love blossomed, growing deeper and stronger with each passing day. They often laughed about their serendipitous encounter – the day they skillfully avoided a potentially embarrassing situation. And as they reminisced about that day, they couldn't help but be grateful for the unexpected circumstances that had brought them closer together.As time went by, Sophie learned that life was full of surprises. Sometimes, the greatest love stories were the ones that were built on a foundation of spontaneity, curiosity, and a willingness to embrace the unexpected. And so, their adventurous journey continued, navigating the twists and turns of life, hand in hand, ready to face whatever challenges came their way.Chapter 5: A Journey of DiscoverySophie and Jack's love continued to thrive as they embarked on new adventures together. They discovered a shared sense of wanderlust and decided to explore the world. Their first destination was the enchanting city of Paris, known as the "City of Love."As they strolled along the romantic streets of Paris, hand in hand, their hearts were filled with excitement and wonder. They wandered through the charming neighborhoods, admiring the stunning architecture and indulging in delicious pastries from local bakeries.One evening, as they stood beneath the Eiffel Tower, bathed in its golden glow, Jack took Sophie's hand and whispered, "This is where I fell in love with you, Sophie. I can't imagine my life without you." Tears welled up in Sophie's eyes as she realized howdeeply Jack cared for her. With joyous tears streaming down her face, she replied, "I feel the same way, Jack. You are my greatest adventure, and I am so grateful to have you by my side."Chapter 6: A Proposal Under the StarsTheir journey continued, and soon they found themselves on a secluded island in the Caribbean. As they walked along the sandy beach, with the gentle waves lapping at their feet, the night sky filled with millions of twinkling stars.Jack pulled Sophie close, his voice filled with love and excitement. "Sophie, my love, I have a question to ask you," he whispered, dropping down on one knee as astonishment filled Sophie's face. With trembling hands, he pulled out a small velvet box, opening it to reveal a stunning diamond ring. "Will you marry me?"Sophie's heart skipped a beat, and tears of joy streamed down her face as she nodded eagerly, unable to find her voice. They embraced under the sparkling sky, sharing a moment of unadulterated happiness as they sealed their love with a passionate kiss.Chapter 7: The Wedding of a LifetimeSophie and Jack's wedding day arrived, and they were surrounded by friends and family who had witnessed their love story unfold. The ceremony took place in a picturesque garden, adorned with blooming flowers and draped in delicate lace. The scent of roses floated in the air, creating a fragrance that would forever be etchedin their memories.As Sophie walked down the aisle, her heart overflowed with love and gratitude. Jack's eyes locked onto hers, and a radiant smile graced his face. Tears of happiness filled Sophie's eyes as they exchanged heartfelt vows, promising to love and support each other for the rest of their lives.The reception was a joyful celebration of their love, filled with laughter, music, and heartfelt toasts. Sophie and Jack danced their first dance as a married couple, their steps in perfect harmony, symbolizing the journey they had taken together.Chapter 8: A Love That EnduresYears passed, and Sophie and Jack's love only intensified. They faced challenges and triumphs, but their commitment to each other remained steadfast. They built a beautiful life together, filled with love, laughter, and shared dreams.As they grew old together, Sophie and Jack often found themselves reminiscing about that fateful day in the park – the unexpected encounter that had led them to a lifetime of love and adventure. They realized that life had a funny way of bringing people together, and their story was a testament to the power of love and the beauty of embracing the unexpected.Sophie and Jack's love story was a testament to the fact that true love could withstand the tests of time. They had learned that loveis not just about the grand gestures and passionate moments butalso about the quiet, everyday moments that bring two souls closer together.And so, their love endured. Through the winds of change and the challenges of life, Sophie and Jack walked hand in hand, their hearts forever intertwined, cherishing each precious moment they shared.。
HYSYS再沸器模拟-推荐下载
For the distillation column and the reboiled absorber, the following predefined reboiler pre-configurations (below) are available on page 2 of the column configuration wizard. Depending on the configuration you can specify a HYSYS Reboiler, a Heater, or a heat exchanger model as the reboiler type.Thermosiphon reboilers can now be simulated rigorously using Aspen Shell & Tube Exchanger (formerly Tasc+). Using this operation as the reboiler you can select different types of EDR-Shell and Tube heat exchangers such as thermosiphon, kettle, and forced circulation.The heat exchanger is integrated with the column so that rigorous calculations can be done in both the column and the reboiler. The heat exchanger is treated as part of the column and both the column and the heat exchanger are solved simultaneously.When the selections are complete move to the next page to specify top and last stage pressures.Notes:∙For a thermosiphon reboiler, both fixed-flow and find-flow options are implemented.∙Some specs related to the EDR-Shell and Tube heat exchanger are automatically defined to minimize user input.∙ A damping factor is added for EDR-Shell and Tube heat exchangers inside the column sub-flowsheet to improve theconvergence of the solver.Each configuration is shown both as an approximation of the way it might be arranged in an actual column and in detail showing how it is represented using heat exchangers, flash drums, mixers, and splitters. In these diagrams, NT represents the number of trays (stages)Reboiler Once-throughThis configuration is available when using the kettle reboiler. The liquid coming from the stage above the reboiler passes through the reboiler once and is returned to the bottom of the column as amixture of liquid (which goes into the sump, to be drawn as bottom product) and vapor (which goes up to the stage above).Reboiler Circulation withoutbaffleThe liquid coming from the stage above the reboiler enters the sump, from which the bottoms and the reboiler feed are both drawn (with the same composition). The reboiler product is returned above the sump. Vapor may also be produced when the hot liquid return from the reboiler contacts the liquid in the sump.Reboiler Circulation with baffleThe sump is divided into two sections with different compositions. The liquid from above the reboiler enters one section, from which the reboiler feed is drawn. The reboiler liquid product enters the other section, from which the bottoms stream is drawn.Excess reboiler liquid return (above the flow rate of the bottoms stream) overflows into the first section. Vapor from the first section (along with that from the reboiler product) passes up to the stage above.。
FlowComp
Kit ContentsFlowComp ™ Dynabeads ® contains ~1 × 109 (~10 mg) beads/mL in phosphate buffered saline (PBS), pH 7.4, with 0.1% bovine serum albumin (BSA) and 0.02% sodium azide as a preservative. FlowComp ™ Human CD3 Antibody contains monoclonal CD3 antibody in PBS with 0.5% BSA and 0.02% sodium azide. FlowComp ™ Release Buffer contains modified biotin in 0.1% BSA and 2 mM EDTA.Caution: Sodium azide may react with lead and copper plumbing to form highly explosive metal azides.Product DescriptionFlowComp ™ Human CD3 is intended for positive magnetic isolation of CD3+ T cells from human peripheral bloodmononuclear cells (PBMCs). The isolated cells are highly pure, viable, and bead-free (fig. 1). In the first step, FlowComp ™ Human CD3 Antibody is added and binds to the target cells. In the second step, CD3+ T cells, that have bound the specific antibodies, are captured by the FlowComp ™ Dynabeads ®. In the third and last step, the cells are released from the FlowComp ™ Dynabeads ®.Dynabeads ® FlowComp ™ Human CD3Isolation from PBMCFor research use only. Not for human or animal therapeutic or diagnostic use.Catalog no. 11365D Store at 2˚C to 8˚CRev. Date: February 2012 (Rev. 001)Downstream ApplicationsIsolated cells are bead-free and may be used directly in anydownstream application including flow cytometry. The cells readily proliferate in response toDynabeads ® Human T Activator CD3/CD28 and can be measured by incorporating EdU or in a CFSE assay.Required Materials• Magnet (DynaMag ™ portfolio).See /magnets for recommendations.• Mixer allowing tiltingand rotation of tubes (e.g. HulaMixer ® Sample Mixer).• Isolation Buffer:Ca 2+ and Mg 2+ free PBSsupplemented with 0.1% BSA and 2 mM EDTA.Note: BSA can be replaced by human serum albumin (HSA) or 2% FBS/FCS.• Optional: Flow cytometry antibodies. We recommend using anti-CD3clone UCHT-1 from Caltag Medsystems as primary fluorescent antibody for flow staining of cells after isolation. Optional clones: OKT3, HITa3.• Optional: For viability analysis, SYTOX ® Red is recommended.General Guidelines• Visit /cellisolation and follow ourQuickLinks for recommended sample preparation procedures.• Use a mixer that provides tilting and rotation of the tubes to ensure thatbeads do not settle in the tube.• This product should not be used with the MPC ™-1 magnet(Cat. no. 12001D).• Avoid air bubbles (foaming) during pipetting.• Never use less than the recommended volume of beads.• Carefully follow the recommended pipetting volumes and incubationtimes.• Keep all buffers cold.• To avoid unspecific labeling of cells during flow staining, werecommend using gammaglobulin prior to staining with primary fluorescent antibody.• For better purity, repeat the washing step once or transfer the bead-bound cells to a new tube before adding the FlowComp ™ Release Buffer.• All incubations at room temperature can also be performed at2°C to 8°C.ProtocolThis protocol is intended for isolation of bead-free CD14+ monocytes, starting with 5 × 107 PBMC/test where typically 10–20% are CD14+ monocytes. When working with higher cell numbers/number of tests, scale up all volumes accordingly, as shown in Table 1.Wash the BeadsSee Table 1 for volume recommendations.1. Resuspend the beads in the vial (i.e. vortex for >30 sec, or tilt and rotatefor 5 min).2. Transfer the desired volume of beads to a tube.3. Add the same volume of Isolation Buffer, or at least 1 mL, andresuspend.4. Place the tube in a magnet for 1 min and discard the supernatant.5. Remove the tube from the magnet and resuspend the washed beadsin the same volume of Isolation Buffer as the initial volume of beads (step 2).Prepare Cells• Prepare a PBMC suspension according to “General Guidelines”.Resuspend the cells at 1 × 108 cells/mL in Isolation Buffer. • Prepare approximately 10 mL of Isolation Buffer per 5 × 107cells.PBMC: ~2 × 109Figure 1: Purity of human CD3+ cells isolated from PBMC using Dynabeads ® FlowComp ™Human CD3.For support visit /support or *****************************©2012 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners, except where otherwise stated. LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) DISCLAIM ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. IN NO EVENT SHALL LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF .SPEC-06682on labels is the symbol for catalog number.REF Table 1: Volumes for human CD3+ T cells. This protocol is scalable from 1 × 107 to 5 × 108 PBMC. larger tube than recommended in step 14 to successfully remove the biotin in the sample.** When incubating, tilt and rotate the vial so the cells and beads are kept in the bottom of the tube. Do not perform end-over-end mixing if the volume is small relative to the tube size.Description of MaterialsFlowComp ™ Dynabeads ® are uniform, superparamagnetic polystyrene beads (2.8 μm in diameter) coated with modified streptavidin. FlowComp ™ Human CD3 Antibody contains a DSB-X conjugated monoclonal mouse anti-human CD3. FlowComp ™ Release Buffer contains a modified biotin that displaces the modified biotin on the antibody to release cells from the beads.Limited Use Label LicenseThe purchase of this product conveys to the purchaser the limited, nontransferable right to use the purchased amount of the product only to perform internal research for the sole benefit of the purchaser. No right to resell this product or any of itscomponents is conveyed expressly, by implication, or by estoppel. This product is for internal research purposes only and is not for use in commercial applications of any kind, including, without limitation, quality control and commercial services such as reporting the results of purchaser’s activities for a fee or other form of consideration. For information on obtaining additional rights, please contact outlicensing@ or Out Licensing, Life Technologies, 5791 Van Allen Way, Carlsbad, California 92008.Manufactured by Life Technologies AS, Norway. Life Technologies AS complies with the Quality System Standards ISO 9001:2008 and ISO 13485:2003.Limited Product WarrantyLife Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the Life Technologies' General Terms and Conditions of Sale found on Life Technologies' website at /termsandconditions . If you have any questions, please contact Life Technologies at /support .Isolate CellsThis protocol is based on 5 × 107PBMC, but is directly scalable from 1 × 107to 5 × 108 cells, according to Table 1.1. Transfer 500 μL (5 × 107 cells) prepared cells to a tube and add 25 μLFlowComp ™ Human CD3 antibody. 2. Mix well and incubate for 10 min at 2°C to 8°C.3. Wash by adding 2 mL Isolation Buffer and centrifuge for 8 min at350 × g.4. Remove the supernatant and resuspend in 1 mL Isolation Buffer.5. Add 75 μL washed FlowComp ™ Dynabeads ® and mix well(e.g. vortex 2–3 seconds).6. Incubate for 15 min at room temperature under rolling and tilting.7. Add 1 mL isolation buffer, pipet 2–3 times (or vortex 2–3 seconds) andplace the tube in a magnet for 2 min.8. While the tube is still in the magnet, carefully remove and discard thesupernatant containing the CD3 negative cells.9. Repeat steps 7–8 to wash the bead-bound CD3+ cells. These steps arecritical to obtain a high purity of isolated cells.Release Cells10. Resuspend the bead-bound cells in 1 mL Release Buffer.11. Incubate for 10 min with rolling and tilting at room temperature.12. Pipet 10 times to efficiently release the cells and place in a magnet for2 min. Avoid foaming.13. Transfer the supernatant containing the bead-free CD3+ cells to a newtube, and again place on the magnet for 1 min to remove any residual beads. Transfer again the supernatant containing the bead-free cells to a new tube.14. Add 2 mL Isolation Buffer followed by centrifugation for 8 min at350 × g. Discard the supernatant and resuspend the cell pellet in preferred medium.Keep the cells on 2°C to 8°C until further use in downstream applications.Related Products。
生物膜(英文)
9,12,15-octadecatrienoate)
缩醛磷脂能量
缩醛磷脂能量下载温馨提示：该文档是我店铺精心编制而成，希望大家下载以后，能够帮助大家解决实际的问题。
文档下载后可定制随意修改，请根据实际需要进行相应的调整和使用，谢谢!并且，本店铺为大家提供各种各样类型的实用资料，如教育随笔、日记赏析、句子摘抄、古诗大全、经典美文、话题作文、工作总结、词语解析、文案摘录、其他资料等等，如想了解不同资料格式和写法，敬请关注!Download tips: This document is carefully compiled by the editor. I hope that after you download them, they can help yousolve practical problems. The document can be customized and modified after downloading, please adjust and use it according to actual needs, thank you!In addition, our shop provides you with various types of practical materials, such as educational essays, diary appreciation, sentence excerpts, ancient poems, classic articles, topic composition, work summary, word parsing, copy excerpts,other materials and so on, want to know different data formats and writing methods, please pay attention!缩醛磷脂是一种重要的生物分子，在细胞膜的结构和功能中起着关键作用。