5569 Nuclear A4+(1) - 1 Oct 2010 proof

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fugene 4k 转染试剂 中文说明书

fugene 4k 转染试剂 中文说明书

2022版 CTM694原英文技术手册TM694中 文 说 明 书适用产品目录号:E5911和E5912FuGENE ®4K TransfectionReagent普洛麦格(北京)生物技术有限公司Promega (Beijing) Biotech Co., Ltd 地址:北京市东城区北三环东路36号环球贸易中心B座907-909电话:************网址:技术支持电话:400 810 8133技术支持邮箱:*************************CTM 6942022制作1所有技术文献的英文原版均可在/ protocols 获得。

请访问该网址以确定您使用的说明书是否为最新版本。

如果您在使用该试剂盒时有任何问题,请与Promega 北京技术服务部联系。

电子邮箱:*************************1. 描述 (2)2. 产品组分和储存条件 (2)3. 一般注意事项 (2)3. A. 转染试剂与DNA的比例 (3)3. B. DNA (3)3. C. 时间 (3)3. D. 血清 (3)3. E. 细胞培养条件 (3)3. F. 稳定转染 (3)4. 推荐操作步骤 (4)4. A. 细胞铺板 (5)4. B. FuGENE® 4K Transfection Reagent准备 (5)4. C. 一般转染操作步骤 (6)4. D. 稳定转染操作步骤 (8)4. E. 转染优化 (9)4. F. 报告基因活性和细胞健康的多重检测方案 (11)5. 疑难解答 (12)FuGENE® 4K Transfection Reagent普洛麦格(北京)生物技术有限公司Promega (Beijing) Biotech Co., Ltd 地址:北京市东城区北三环东路36号环球贸易中心B座907-909电话:************网址:技术支持电话:400 810 8133技术支持邮箱:*************************CTM 6942022制作21. 描述FuGENE® 4K Transfection Reagent是一个多组分,非脂质体试剂,用于将DNA高效、低毒地转染至多种哺乳动物细胞系中,无需在加入试剂-DNA复合物后更换培养基。

核内体定位荧光染料

核内体定位荧光染料

核内体定位荧光染料
核内体定位荧光染料是一种用于标记和定位细胞核内体结构的荧光染料。

细胞核内体是细胞核内的特定区域或结构,常常与基因表达、DNA修复和转录调控等生物学过程密切相关。

常见的核内体定位荧光染料包括:
1. DAPI(4',6-二胺-2-苯基-indole):DAPI是一种DNA结合荧光染料,可以顺利穿透细胞膜和核膜,与DNA结合形成蓝色荧光,用于标记和观察细胞核。

2. Hoechst染料:Hoechst染料系列是一类与核酸结合的荧光染料,可用于核内体的定位和染色。

常见的Hoechst染料包括Hoechst 33342和Hoechst 33258。

3. SYTOX染料:SYTOX染料是一类高亲和力DNA结合的荧光染料,可以用于细胞核内运动的核茎环和组蛋白复合物的观察。

4. Acridine Orange(AO):AO是一种DNA/RNA双链荧光染料,可以用于核内体的定位和染色。

5. propidium iodide(PI):PI是一种DNA结合荧光染料,用于细胞凋亡、细胞周期和细胞核破裂等核内体相关实验。

这些核内体定位荧光染料能够提供细胞核内体结构的明亮和清
晰的荧光信号,帮助研究人员观察和研究细胞核内体的形态和功能。

伏隔核的分子标志物

伏隔核的分子标志物

伏隔核的分子标志物伏隔核(Nuclear Pore Complex,NPC)是一种位于细胞核孔膜上的大型复合蛋白机器,它在细胞核膜上形成了许多核孔,起到了核与细胞质之间物质运输的通道。

伏隔核的功能主要依赖于其组成的复合蛋白,这些蛋白分子作为伏隔核的标志物,对其功能和结构起到了重要的调控作用。

本文将从分子标志物的角度介绍伏隔核的结构和功能。

一、Nup153Nup153是伏隔核的一个重要组成部分,它是一种大型的蛋白质,质量约为153kDa。

Nup153可通过与其他伏隔核蛋白相互作用,形成一个稳定的核孔复合物,参与细胞核与细胞质之间的物质运输。

研究发现,Nup153与核转录因子、mRNA和多种细胞信号分子的结合有关,这些相互作用在基因表达调控和细胞信号传导中起到了重要的作用。

二、Nup62Nup62是伏隔核膜上的一个重要蛋白质,质量约为62kDa。

它的主要功能是参与核孔的组装和稳定,以及调控核-质转运的选择性。

Nup62通过与其他伏隔核蛋白相互作用,形成了一个稳定的核孔复合物。

研究表明,Nup62在核转录因子的核-质转运过程中起到了重要的作用,它能够选择性地调控转运的物质,维持细胞核与细胞质内物质的平衡。

三、Nup98Nup98是伏隔核膜上的一个重要蛋白质,质量约为98kDa。

它在伏隔核的功能中起到了多种作用,包括调控核孔的组装、核-质转运的选择性和细胞周期的调控等。

研究发现,Nup98与一些转录因子和调控因子相互作用,参与了基因表达调控的过程。

此外,Nup98还能够调控细胞周期的进行,维持细胞的正常分裂。

四、Nup214Nup214是伏隔核膜上的一个重要蛋白质,质量约为214kDa。

它是伏隔核的一个关键组分,参与了核-质转运的选择性和细胞周期的调控等功能。

研究发现,Nup214与一些转录因子和RNA结合蛋白相互作用,调控基因的转录和mRNA的转运。

此外,Nup214还能够调控细胞周期的进行,维持细胞的正常分裂。

实验具体步骤结果

实验具体步骤结果
综合性实验 具体安排
确定目标基因,目标生 物DNA提取
实 目标基因PCR扩增 验 PCR产物纯化 思 pUC19骨架进行重组 路 转化大肠杆菌
重组子筛选
琼脂糖电泳 琼脂糖电泳
蓝白斑筛选 PCR\电泳
具体安排
理解实验原理(第9周) 第11周 每个小组交一份实验计划 第12周 DNA提取和PCR 第13周 DNA纯化和重组转化 第14周 PCR验证重组子
CG8864 length 237bp PrimerF3 5’- acacaggaaacagctatgaccatgattacgcca gttaacatccgcagccaact PrimerR3 5’- cacgacgttgtaaaacgacggccagtgaattcg acccacacagaggcacctac
第13周 DNA纯化、重组和转化
第13周 DNA纯化、重组和转化
(灭菌)
(1 uL)
(1 uL) (4 uL)
第13周 DNA纯化、重组和转化
转化 建议所使用的感受态细胞效率≥1×108cfu/μg. 1)冰上融化一管100 μl的DH5a感受态细胞,加入5μl已稀释的 的反应液到感受态细胞中,轻轻混匀,冰上孵育30分钟。 2)42℃水浴中热激90秒后快速放入冰上3分钟。 3)加入500μl SOC液体培养基,37℃复苏45-60分钟。 4)9,000rpm离心3分钟收集菌体,根据需要将一定量的菌体均 匀的涂布在含抗生素平板(Amp. 50-100 ug/mL)上。 5) 37 ℃培养18-24h后,平板置于4 ℃保存。
实验计划要求
选定扩增基因 每个组4个人,可以选做两个基因片段
(建议每两个人做一个基因片段)
每步工作的具体负责人和主要任务

400M核磁共振谱仪

400M核磁共振谱仪

上海工程技术大学教育研究 3/2007400M 核磁共振谱仪Nuclear Magnetic Resonance Spectrometer国别:瑞 士设备价格:21.6万美元联系人:任新峰购置日期:2006年6月设备所在地:实训楼3423联系电话:67791221设备简介:核磁共振是指原子核在静磁场中的作用下对固定频率的射频电磁波进行吸收的现象。

核磁共振广泛应用于化学、生物、医学等领域。

核磁共振的方法与技术作为分析物质的手段,由于其可深入物质内部而不破坏样品,并具有迅速、准确、分辨率高等优点而得以迅速发展和广泛应用,已经从物理学渗透到化学、生物、地质、医疗以及材料等学科,在科研和生产中发挥了巨大作用。

核磁共振(Nuclear M agnetic Resonance,NMR)技术在过去的六、七十年的过程中得到了非常快速的发展。

特别是在有机化学、生物化学等领域是一个非常有力的工具。

技术参数:电源:220V(10%,50Hz)操作室温度:15~30 操作持续时间:连续操作相对湿度:<85%控温设置范围:-150~350 控温精度:0.1 /1H 灵敏度: 220!1(0.1%EB)13C 灵敏度: 160!1(ASTM)15N 灵敏度: 20!1(90%form amide)∀61∀31P灵敏度:135!1(T PP)变温范围:-150~150Z-梯度场强度:50g/cm分辨率:#0.45Hz(3%CHCL)应用范围:核磁共振波谱仪是化学、化工、制药、食品、生命科学、生物工程等领域中进行化合物的结构测定所不可缺少的大型分析仪器,用于化学化工学科教学、科研和研究生培养工作。

核磁共振波谱仪是四大光谱分析仪器之一,也是一种权威的结构鉴定手段的首选仪器。

(上接第60页)应用范围:高效液相色谱仪是化学、化工、制药、食品、生命科学、生物工程等领域中进行化合物的定性和定量分析所不可缺少的分析仪器。

主要可用于精细化工产品成分的定性和定量分析,精细有机合成、催化及反应工程、高分子材料化学、纳米材料的物理与化学特性、功能与生物材料等的研究,是用于化学化工学科教学、科研和研究生培养工作的重要测试仪器之一。

化学发光法检测传染病优秀课件优选ppt资料

化学发光法检测传染病优秀课件优选ppt资料
第十一页,共48页。
AutolumiS2000微粒子化学发光仪
第十二页,共48页。
第十三页,共48页。
全自动化学发光免疫分析(fēnxī)系统开发及其产业化,在2021 年12月通过国家科学技术委员会的鉴定,鉴定委员会认为:
该项目具有创新 性和自主(zìzhǔ)知识 产权,整体达到国际 先进水平。
❖ 批内、批间的分析重现性。
第二十二页,共48页。
微粒子化学发光试剂(shìjì)的精密度
HCV
1
2
3
4
5
…… Mean SD CV
批内 86.11 86.36 88.40 87.90 86.43 …… 86.27 1.55 1.79%
批间 74.59 73.63 69.64 71.90 71.96 …… 71.01 3.20 4.50%
S1, S2阴性, S3,S4,S5,S6阳性
L1、L2阳性, L3阴性,L4阴性
传统酶免法 特异性
(-/-)20/20 (+/+)3/3
(-/-)20/20 (-/-)15/15 (+/+)9/10
(-/-)15/15 (+/+)9/10
(-/-)15/15 (+/+)14/15
(-/-)29/30 (+/+)29/30 (-/-)18/20 (+/+)20/20 (-/-)20/20 (+/+)10/10
4.91% 2.14%
HBsAg
试剂(shìjì)性能多中心临床评估
HIV
HCV
相关性 显著相关 显著相关
相关系数 0.99 0.93
线性系数 0.98 0.99

生物素标记EMSA探针-AP1

生物素标记EMSA探针-AP1

⽣物素标记EMSA探针-AP1⽣物素标记EMSA探针-AP1产品简介:⽣物素标记EMSA探针-AP1是⽤于EMSA(也称gel shift)研究的并经⽣物素(Biotin)标记的AP1 consensus oligonucleotide。

这个⽣物素标记的双链寡核苷酸含有公认的AP1结合位点,可以⽤作EMSA研究时的探针。

AP1 consensus oligo的序列如下:5’-CGC TTG ATG ACT CAG CCG GAA-3’3’-GCG AAC TAC TGA GTC GGC CTT-5’本⽣物素标记EMSA探针已经过纯化,可以直接⽤于EMSA结合反应。

本⽣物素标记EMSA探针可以和碧云天的化学发光法EMSA试剂盒(GS009)配套使⽤。

⼀个包装的⽣物素标记探针可以进⾏约200-400个样品的EMSA检测。

保存条件:-20℃保存,⼀年有效。

注意事项:避免加热到40℃以上,温度过⾼会导致双链DNA探针解聚成单链。

⽽单链⽆法⽤于EMSA研究。

对于基于⽣物素标记的EMSA检测的详细操作可以参考碧云天的化学发光法EMSA试剂盒(GS009)的使⽤说明。

为了您的安全和健康,请穿实验服并戴⼀次性⼿套操作。

使⽤说明:1.本⽣物素标记EMSA探针⽤于EMSA结合反应时,参考如下步骤进⾏:A.如下设置EMSA结合反应:阴性对照反应:Nuclease-Free Water 7-7.5µl EMSA/Gel-Shift 结合缓冲液(5X) 2µl 细胞核蛋⽩或纯化的转录因⼦ 0µl ⽣物素标记探针 0.5-1µl 总体积 10µl探针冷竞争反应:Nuclease-Free Water 4-4.5µl EMSA/Gel-Shift 结合缓冲液(5X) 2µl 细胞核蛋⽩或纯化的转录因⼦ 2µl 未标记的探针 1µl ⽣物素标记探针 0.5-1µl 总体积 10µlSuper-shift反应:Nuclease-Free Water 4-4.5µl EMSA/Gel-Shift 结合缓冲液(5X) 2µl 细胞核蛋⽩或纯化的转录因⼦ 2µl ⽬的蛋⽩特异抗体 1µl ⽣物素标记探针 0.5-1µl 总体积 10µl 样品反应:Nuclease-Free Water 5-5.5µl EMSA/Gel-Shift 结合缓冲液(5X) 2µl 细胞核蛋⽩或纯化的转录因⼦ 2µl ⽣物素标记探针 0.5-1µl 总体积 10µl突变探针的冷竞争反应:Nuclease-Free Water 4-4.5µl EMSA/Gel-Shift 结合缓冲液(5X) 2µl 细胞核蛋⽩或纯化的转录因⼦ 2µl 未标记的突变探针 1µl ⽣物素标记探针 0.5-1µl 总体积 10µl注:⽣物素标记EMSA探针的推荐⽤量为每个反应0.5微升,如果检测出来的⽬的蛋⽩的EMSA条带偏弱,可以适当加⼤⽣物素标记EMSA探针的⽤量⾄0.75微升或1微升。

应用凝集素芯片检测胃正常黏膜上皮细胞与胃癌细胞膜表面糖链表达

应用凝集素芯片检测胃正常黏膜上皮细胞与胃癌细胞膜表面糖链表达

应用凝集素芯片检测胃正常黏膜上皮细胞与胃癌细胞膜表面糖链表达LI Chun-hui;HE Qun;MA Jing-hong;PAN Zhong-cheng;WANG Tian-jiao;ZHANG Yu-kui;ZHAO Yu-jie【期刊名称】《中国医科大学学报》【年(卷),期】2009(038)011【摘要】目的应用凝集素芯片检测胃正常黏膜上皮细胞系GES-1和胃癌细胞系SGC7901、MKN45细胞膜表面糖链表达,并对芯片上捕获的细胞进行初步的免疫荧光研究.方法用胶原酶Ⅱ消化细胞系,利用芯片上的凝集素位点对糖链的亲和特异性捕获荧光标记的细胞,激光共聚焦扫描仪扫描检测细胞膜表面糖链表达.结果在大多数凝集素位点上,这几种细胞均显示出荧光信号;在凝集素位点WBA、EEL、MAH-Ⅱ上均未显示荧光信号;其中,与GES-1相比,胃癌细胞系SGC7901和MKN45在LTL、HHL位点上基本没有荧光信号.结论根据凝集素的亲和特异性说明,这几种细胞系膜表面共同表达的糖链包括:唾液酸、乙酰葡萄糖/葡萄糖、乙酰半乳糖/半乳糖、甘露糖等糖链.其中,胃癌细胞系SGC7901和MKN45可能较少表达岩藻糖.【总页数】4页(P807-809,813)【作者】LI Chun-hui;HE Qun;MA Jing-hong;PAN Zhong-cheng;WANG Tian-jiao;ZHANG Yu-kui;ZHAO Yu-jie【作者单位】Biochip Center,Key Laboratory of Cell Biology,Ministry of Public Health of China,China Medical University,Shenyang110001,China;Biochip Center,Key Laboratory of Cell Biology,Ministry of Public Health of China,China Medical University,Shenyang110001,China;Biochip Center,Key Laboratory of Cell Biology,Ministry of Public Health of China,China Medical University,Shenyang110001,China;Biochip Center,Key Laboratory of Cell Biology,Ministry of Public Health of China,China Medical University,Shenyang110001,China;Biochip Center,Key Laboratory of Cell Biology,Ministry of Public Health of China,China Medical University,Shenyang110001,China;Biochip Center,Key Laboratory of Cell Biology,Ministry of Public Health of China,China Medical University,Shenyang110001,China;Biochip Center,Key Laboratory of Cell Biology,Ministry of Public Health of China,China Medical University,Shenyang 110001,China 【正文语种】中文【中图分类】R392.12【相关文献】1.应用凝集素芯片检测细胞膜表面糖链种属特异性 [J], 马汝海;王天骄;潘忠诚;何群2.应用凝集素芯片检测兔组织细胞膜表面糖谱 [J], 何群;张艳;宋春阳;潘忠诚;王天骄;张玉魁;赵雨杰3.应用凝集素芯片检测非小细胞肺癌细胞膜表面糖链表达 [J], 李春辉;何群;潘忠诚;王天骄;张玉魁;李超;赵雨杰4.应用凝集素芯片分析癌细胞膜表面糖链表达 [J], 李春辉;何群;马静红;潘忠诚;王天骄;张玉魁;赵雨杰5.胃康舒宁对胃癌前病变大鼠胃黏膜上皮细胞凋亡及其相关调控基因表达的影响[J], 刘旺根;王守东;冯黎因版权原因,仅展示原文概要,查看原文内容请购买。

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Health,safety and the environment The Weir Group’s policy on health and safety requires that all our companies take a proactive responsible attitude to the protection of their employees’health and safety.The driving force behind our performance continues to be our emphasis on behaviour,networking and sharing of best practice and the active involvement of senior management to promote and audit safety programmes.All our locations fully integrate environmental management into their operational systems and procedures.Weir’s proactive approach ensures that these processes reduce our environmental impact year on year.Engineered Valves for Nuclear Power Generation3Quality assuranceWeir operates quality programmes to cover the full scope of our activities,and deploys quality systems that have been developed specifically to serve the nuclear market.Weir is qualified to industry standards and working practices including:ASME BPVC Section III (N and NPT Stamp)ASME BPVC Section VIII (UV Stamp)NQA-1Quality system 10CFR50App.B 10CFR 21RCC-E RCC-M CSA Z299OTT 87Performance testing and qualification to:ASME QME-1ASME B16.41IEEE 323IEEE 344IEEE 382ISO 9001ISO 14001ISO 17025PED 97/23/CEAPI Q1TO API LICENCES:API 6D (6D-0182)API 6A (64-0445)TUV -AD MERKBLATT WRD HP 0ATEX 94/9/CELean manufacturingpracticesCONTENTSIntroduction 2-3Butterfly valves 4-5Check valves 6-7Control valves8Gate valves -Parallel slide 9Gate valves -Wedge design 10Globe valves -T pattern 11Globe valves -Y pattern 12Safety valves13Special Applications Main steam isolation valves15-16Compact integrated safety valves17Integrated single safety relief valves 18Isolation,safety and relief valves 19Main steam safety valves 20Tandem safety relief valves 21Nuclear valve service224Engineered Valves for Nuclear Power GenerationTriple offset metal seated butterfly valvesDesign standards:ASME Section III ASME B16.34Sizes:3-42inch (DN 73-1067)Pressure class:Class 150-600Applications:Containment isolation,vent and purge Equipment isolation Service water CondensateCirculating water Features:Metal to metal seating uses no elastomers,allowing for high temperature service and resistance to radiationTriple offset design creates no rubbing,galling or wear,leading to long life and low maintenanceValve is torque seated in the closed direction,resulting in tight shutoff Bi-directional tight sealing Materials of construction:Carbon steels Alloy steels Stainless steels AL6XN End connections:Flanged Lugged Wafer Special Actuator options:Pneumatic Electric motor Gas-hydraulic Manual gear Bare stem Options:Position indication Stem instrumentationEngineered Valves for Nuclear Power Generation5High performance butterfly valvesDesign standards:ASME B16.34Sizes:3-80inch (DN 80-2600)Pressure class:150-600Applications:Process controlEquipment isolationFeatures:Resilient seated,metal seated,firesafe and swingthrough optionsSingle and double offset shaft design Varidiff anti cavitation trim Materials of construction:Body and vane:•Carbon and alloy steels•Stainless and duplex stainless steels •Copper,nickel alloys and titanium End connections:Flanged Lugged Wafer Butt weld Actuator options:Pneumatic Electric motor Hydraulic Manual gearButterfly valve with anti-cavitation cowel6Engineered Valves for Nuclear Power GenerationFree flow reverse current valves (FFRCV)Design standards:ASME B16.34Sizes:3-44inch (DN 77-1118)Pressure class:150-600+Applications:Steam turbine protection Extraction steam non-return Turbine non-returnBleeder trip and bled steam non-return Features:Free swinging disc allowsindependent movement of the disc with assured closure upon loss or reversal of flowInclined seat design optimises performance with low pressure drop and fast closure Self aligning disc and disc arm assures repeatable tight sealing Materials of construction:Body and disc:•Carbon steels •Alloy steels •Stainless steels Seat:•Stainless steel overlays •Hardfacing alloys Cover seal design:Bolted bonnet End connections:Butt weld FlangedOptions:Pneumatic or hydraulic power cylinder Limit switchesDisc counterweight Manual testEngineered Valves for Nuclear Power Generation7Swing check valves Lift check valvesDesign standards:ASME Section III ASME B16.34Sizes:3-36inch (DN 80-915)Pressure class:150-2500Applications:FeedwaterEmergency core cooling system Containment isolation Balance of plantFeatures:Swing check:•Two disc-arm designs available:Singlepiece disc-arm and disc ensures that disc will not flutter or loosen over time.Two piece disc-arm and disc promotes repeatable tight sealing •Optional internal shelf bracket designeliminates body penetrations and potential leak paths Lift check:•Available in ‘T’-pattern or lower pressure-drop ‘Y’-pattern configuration•One piece poppet design with hardfacing on guide surfaces•Valves sized on seat bore to ensure full lift in open position Materials of construction:Carbon steels Alloy steels Stainless steels Cover seal designs:Bolted bonnet Pressure seal Body/cover joint End connections:Butt weldOptions:Pneumatic test options (testable check)Position indicationSeal weld (bolted bonnet)Canopy seal (pressure seal)Manual stroke8Engineered Valves for Nuclear Power GenerationControl valvesDesign standards:ASME section III ASME B16.34RCC-MSizes:½-36inch (DN 15-900)Pressure class:150-4500Applications:Process controlFeatures:Single and multi stagepressure drop Severe service trims Stable cage guiding Materials of construction:Body and bonnet:•Carbon steels •Alloy steels •Stainless steels•Any other specified materialsPlug and seats:Hardfacing #6overlay/hardened material depending on pressure drop Seal design:Bolted bonnet with spiral wound gaskets Pressure seal End connections:Flanged or butt weld Actuator options:Pneumatic Electric motor Hydraulic Other options:Fast operationAngle and 3way designsEngineered Valves for Nuclear Power Generation9Parallel slide gate valvesDesign standards:ASME section III ASME B16.34RCC-MSizes:½-36inch (DN 15-900)Pressure class:150-2500Applications:High pressure system isolation Equipment isolationFeatures:Position seated -stops on limitnottorque Wide,flat seating surface No thermal binding issues Bi-directional operation Non gallingConsistent thrustAddresses GL 89-10issues Materials of construction:Body:•Carbon steels •Alloy steels •Stainless steelsSeat and wedge:•Hardfaced with stellite or cobalt free materials Seal design:Bolted bonnet (seal weld provision available)Pressure seal End connections:Butt weld Flanged Actuator options:Pneumatic Electric motor Gas-hydraulic Manual gear Bare stemOther options:Position indication Instrumented stemBonnet overpressure protection Seal weld Canopy weldIntegral bypass arrangements/optional10Engineered Valves for Nuclear Power GenerationWedge gate valvesDesign standards:ASME section III ASME B16.34RCC-MSizes:3-24inch (DN 80-600)Pressure class:150-2500Applications:Containment isolation Equipment isolation Features:Flexible wedge design Bi-directional operationPrecision body guides Tight shutoffAddresses GL 89-10concerns Materials of construction:Body:•Carbon steels •Alloy steels •Stainless steels Seat and wedge:•Hardfaced with stellite or cobalt freematerialsSeal design:Bolted bonnet (seal weld provision available)Pressure seal End connections:Butt weld Flanged Actuator options:Pneumatic Electric motor Gas-hydraulic Manual gear Bare stemOther options:Position indicationBonnet overpressure protection Seal weld (bolted bonnet)Canopy seal (pressure seal)Integral bypass arrangements/option Instrumented stemT pattern globe valvesDesign standards:ASME section III ASME B16.34Sizes:3-24inch (DN 77-610)Pressure class:150-2500Applications:Containment isolationEquipment isolation requiring tight shutoff Features:45degree seatsPrecisionguides with wear resistant coating Large seat bore for reduced pressure drop Materials of construction:Body and poppet:•Carbon steels •Alloy steels •Stainless steels Seat:•Stainless steel overlays •Alloy:Hardfacing #21Seal design:Bolted bonnet PressuresealEnd connections:Butt weld Flanged Actuator options:Pneumatic Electric motor Gas-hydraulic Manual gear Bare stemOther options:Position indicationStop check design available Angle design available Seal weld (bolted bonnet)Canopy seal (pressure seal)Instrumented stemNon-cobalt hardfacing materials availableY pattern globe valvesDesign standards:ASME section III ASME B16.34Sizes:3-24inch (DN 77-610)Pressure class:150-2500Applications:Containment isolationEquipment isolation requiring tight shutoff Features:Y-Pattern for lower pressure lossBevelledseats for tight sealingLarge seat bore for reduced pressure drop Materials of construction:Body and poppet:•Carbon steels •Alloy steels •Stainless steels Seat:•Stainless steel overlays •Hardfacing alloysSeal design:Bolted bonnet (seal weld provision available)Pressure sealEnd connections:Butt weld Flanged Actuator options:Pneumatic Electric motor Gas-hydraulic Manual gear Bare stemOther options:Position indication Instrumented stemNon-cobalt hardfacing materials availableSpring loaded safety relief valves (Starflow,Series 9)Damped spring loaded relief valvesAtmospheric relief valves Pilot operated safety relief valves Changeover valvesSize:½-12inch (DN 15-305)Pressure:From 7to 14500PSI (0.05to 100Mpa)Class:ASME Class 150#–2500#or customised Applications:Suitable for all types of nuclear reactors (PWR,BWR,CANDU PHWR,RBMK LWGR,HTGR)High and low pressure overpressureprotection on steam,water and gas lines Nuclear steam supply system (NSSS)and balance of nuclear island•Steam generator blowdown system •Electrical building chilled water system•Spent fuel pit cooling and treatment system •Reactor coolant system•Chemical and volumetric control system •Reactor boron and water make up system •Nuclear sampling system •Safety injection system•Nuclear island vent and drain system •Reactor heat removal system •Component cooling system•Instrument compressed air distribution system•Nuclear island demineralised water distribution•Gaseous waste treatment system •Boron recycling system•Waste auxiliary components coolingsystemFeatures:Full liftSemi or full nozzle design Metal or soft seat Cast or forged bodyCarbon,alloy or stainless steel Flange,threaded,weldedAvailable with a damping system (GRAB)to prevent acoustic vibrationphenomenaStarflow series with damping systemCast Starflow safety valvesForged stainless steel safety valvesLargest ever main stream isolation valves designed and built at Weir’s UK plant, recently shipped to a nuclear power plant in ChinaGate designMain steam isolation valves (MSIV)Main feedwater isolation valves (MFIV)Design standard:ASME section III RCC-MSizes:14-32inch (DN 356-819)Pressure class:600-1500Applications:Main steam isolation Main feedwater isolationFeatures:Gate design reduces pressuredropQuickclosure design (typically 3-5seconds)Available as parallel slide or wedge gate design Materials of construction:Body:•Carbon steels •Alloy steels •Stainless steelsSeats and precision guides:Hardfacing #6overlay Seal design:Bolted bonnet (seal weld provisionavailable)Pressure seal End connections:Butt weldActuator options:Pneumatic Electric motor Gas-hydraulic Other options:Position indication Stem instrumentationMain steam isolation valveGlobe designMain steam isolation valves (MSIV)Design standard:ASME section III Sizes:4-38inch (DN 100-950)Pressure class:600-1500Applications:Main steam isolationFeatures:Globe designensures tight shutoffQuick closure design (typically 3-5seconds)Simple and effective design -process and springs assist closureCompact valve and actuator design -low centre of gravityAdvanced flow path design for minimal pressure lossNose guide for tight seatingBack seated for stability and resistance to vibration Materials of construction:Body and poppet:•Carbon steels •Alloy steels •Stainless steelsSeats and precision guides:alloy Hardfacing #21overlaySeal design:Bolted bonnet (seal weld provision available)End connections:Butt weld Actuator options:Pneumatic with spring assist Electric motor Other options:Position indication Stem instrumentationDSM 3000Sizes:½-1½inch (DN 15-40)Pressure:From 70to 2900Psi (0,5to 20MPa)Temperature:High temperature applications to 576°C (1069°F)Class:ASME Class 150#–2500#or customised Applications:Suitable for all types of nuclear reactors (submarines,scaled down reactors,experimental reactors,PWR,CANDU PHWR,HTGR)High and low pressure overpressure protection on liquid,steam,gas,and steam/water mixSmall capacity,high speed pressure transient Primary coolant safety valves for submarines Experimental reactors coolant overpressureprotectionFeatures:Excellent operation irrespective of the type of fluid:steam,gas,liquid or two-phase Leak tightness up to the set-pressuremaximizes the operating pressure and output powerNon-flowing pilot valve minimizes ageing of the mechanismAccuracy of pilotset pressure opening value with repeatability better than 1%Soft opening/closing decreases the loads to the installationLow or high pressure in-situ test during operation for preventive maintenance Maintained stability even when flow capacities are well below the maximum rate -no chattering Reduced maintenanceProven and accurate repeatability and reliabilityAvailable remote control -power supply 48,110,220V DC/AC –IP 65–100%operating factorK1-1E qualified for applications within the containment area of nuclear plants Compact designCustomised interfaces (upstream and downstream pipes)Forged stainless steel body –special material on requestIntegrated upstream flange DSMPRG 3090Class 1Safety ValvePRGSizes:1-16inch (DN 25-400)Pressure:From 7to 4300PSI (0.05to 30Mpa)Temperature:High temperature applications to 576°C (1069°F)Class:ASME class 150#–2500#or customised Applications:Suitable for all nuclear reactor designs (PWR,BWR,CANDU PHWR,RBMK LWGR,HTGR)High and low pressure overpressure protection on steam,water,gas or steam/water mixQualified for inside and outside reactor containmentSafety functions during accidental conditions (feed &bleed)Pressuriser safetyvalvesSteam generator safety valves Main steam safety valves Bleed condenser relief valvesReactor heat removal safety valvesReactor volumetric and chemical control circuit overpressure protectionFeatures:Excellent operation irrespective of the type of fluid:steam,gas,liquid or two-phase Leak tightness up to the set-pressure maximises the operating pressure and output powerNon-flowing pilot valve minimises ageing of the mechanismAccuracy of pilot set pressure opening value with repeatability better than 1%Soft opening/closing decreases the loads to the installationLow or high pressure in-situ test during operation for preventive maintenance Maintained stability even when flowcapacities are well below the maximum rate No erosion of sealing faces,no chattering Reduced maintenanceProven and accurate repeatability and reliability Available remote control -power supply 48,110,220V DC/AC –IP 65–100%operating factorK1-1E qualified for applications within the containment area of nuclear plantsProtection against cold overpressure (nuclear vessel protection within the NDTT curve)Compact design reduces size and weight Customised interfaces (upstream and downstream pipes)Prevention of LOCA (Loss of Coolant Accident):the unique feature guarantees reliable closing (Emergency Shut Off (ESO))Steam block unit (SVU)Combines several functions with one main steam isolation valve (SIG series),two pilot operated safety valves (GVG series),and one isolation check valve (CVG series)System medium operated quick closing steam isolation valves:SIG seriesSystem medium operatedisolation check valves:CVG seriesSize:4-34inch (DN 100-800)PressureFrom 70to 2900Psi (0,5to 20MPa)TemperatureHigh temperature applications to 600°C (1112°F)ClassASME Class 600#–900#or customised ApplicationsSuitable for all types of nuclear reactors (PWR,BWR,CANDU PHWR,RBMK LWGR,HTGR)High and low pressure live steam pipeline leading to the turbineMain steam isolation valvesMain steam relief isolation valvesThe Steam Block Unit (SVU)combines several functions•One system medium operated quick closing steam isolation valve (SIG series)intended for steam cut-off in unlikely accidental modes (pipe breaking,primary/secondary leaks)•Two pilot operated safety valves (GVG series)intended for protection of steam generators against overpressure•One isolation check valve (CVG series)intended for cut-off of steam in case of non closing of downstream dump relief valve Features:Guaranteed leak tightness due to system operated principleCustomised to special operational logic Possibility of redundancy of the actuator Low or high pressure in-situ test during operation for preventive maintenance Reduced maintenanceLow power consumption of the actuator -power supply 48,110,220V DC/AC –IP 65–100%operating factorK1-1E qualified for applications within the containment area of nuclear plantsCompact design reduces size and weight Customised interfaces (upstream and downstream pipes)Forged carbon steel body –special material on requestSteam BlockTubeless MSSVMain steam safety valves (MSSV4,MSSV10)Main steam tandem safety valves (MSTSV)GVG seriesSize:1-16inch (DN 25-400)PressureFrom 70to 2900Psi (0,5to 20MPa)TemperatureHigh temperature applications to 576°C (1069°F)ClassASME Class 150#–2500#or customised ApplicationsSuitable for all types of nuclear reactors (PWR,BWR,CANDU PHWR,RBMK LWGR,HTGR)High and low pressure overpressure protection on steam,and steam/water mixturesQualified for Nuclear Steam Supply System (NSSS)Safety functions during accidental conditions (feed &bleed)Steam generator safetyvalvesFeatures:Excellent operation irrespective of the type of fluid:steam,gas,liquid or two-phase Leak tightness up to the set-pressure maximises the operating pressure and output powerNon-flowing pilot valve minimises ageing ofthe mechanismAccuracy of pilot set pressure opening value with repeatability better than 1%Soft opening/closing decreases the loads to the installationLow or high pressure in-situ test during operation for preventive maintenance Maintained stability even when flowcapacities are well below the maximum rate No erosion of sealing faces,no chattering Reduced maintenanceProven and accurate repeatability and reliabilityAvailable remote control -power supply 48,110,220V DC/AC –IP 65–100%operating factorK1-1E qualified for applications within the containment area of nuclear plantsProtection against cold overpressure (nuclear vessel protection within the NDTT curve)Compact design reduces size and weight Customised interfaces (upstream and downstream pipes)Cast or forged carbon steel body –special material on requestThe unique design guarantees reliable closingEngineered Valves for Nuclear Power Generation 21Tandem safety valves,compact tandem safety valves,super compact tandem safety valves Sizes:2-16inch (DN 50-400)Pressure:From 7to 4300PSI (0.05to 30Mpa)Temperature:High temperature applications to 576°C (1069°F)Class:ASME class 150#–2500#or customised Applications:Suitable for all nuclear reactor designs (PWR,BWR,CANDU PHWR,RBMK LWGR,HTGR)High and low pressure overpressure protection on steam,water,gas or steam/water mix Qualified for inside and outside reactor containment Safety functions during accidental conditions (feed &bleed)Pressuriser safety valves Steam generator safety valves Main steam safety valves Bleed condenser relief valves Reactor heat removal safety valves Reactor volumetric and chemical control circuit overpressureprotection Features:Excellent operation irrespective of the type offluid:steam,gas,liquid or two-phaseLeak tightness up to the set-pressuremaximises the operating pressure andoutput powerNon-flowing pilot valve minimises ageing ofthe mechanismAccuracy of pilot setpressure opening valuewith repeatability better than 1%Soft opening/closing decreases the loads tothe installationLow or high pressure in-situ test duringoperation for preventive maintenanceMaintained stability even when flowcapacities are well below the maximum rateNo erosion of sealing faces,no chatteringReduced maintenanceProven and accurate repeatability andreliabilityLow power consumption of the actuator -power supply 48,110,220V DC/AC –IP 65–100%operating factorK1-1E qualified for applications within thecontainment area of nuclear plantsProtection against cold overpressure (nuclearvessel protection within the NDTT curve)Compact design reduces size and weightCustomised interfaces (upstream anddownstream pipes)Prevention of LOCA (Loss of Coolant Accident):the unique TANDEM design guarantees reliableclosingCompact tandemsafety valves22Engineered Valves for Nuclear Power Generation Weir’s nuclear valve aftermarket solutions are based on our OEM engineering anddesign heritage.We apply our engineering expertise to maintenance strategies,station outages,power uprate and life extension projects,with the know-how to work on our own and other OEM equipment.Engineering service capabilities:Studies of valve behaviourFailure modes and effect analysisAutomatic measurement system for openingand closing set pointsReverse engineering of obsolete or non OEMitemsThe core of any effective valve service program is the skill and experience of the technicians and supervisors who come to your site.Our on-site nuclear services include:Maintenance planning and execution Valve outage management In-situ valve seat replacement Installation and commissioning Inventory control and spares availability Life extension,upgrade and refurbishment Provision of specialist tools and test rigs Customised training Benefits:Extensive references and proven track record Detailed and full system understanding Reliability centred maintenance All Weir valve technicians have current nuclear security badging。

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