CommassieBlueFastStainSolution8mins考马斯亮蓝快速染色液
IsatPhone Pro 手机说明书
IsatPhone Pro is the most robust handset in the market. It is dust, splash and shock-proof, with an ingress protection rating of IP54. The handset can operate at the widest extremes from -20°C to +55°C. It can also tolerate humidity from 0 to 95 per cent.
It also includes various applications: USB drivers required to connect your IsatPhone Pro to a laptop; a firmware upgrade tool; a contact synchronisation tool.
What are the plans around SPS migration?
Inmarsat does not plan to close SPS-based services until global variants have been launched to replace them. In this context, Inmarsat intends to support the SPS-based versions of IsatPhone, LandPhone and FleetPhone until at least 2011.
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考马斯亮蓝染色
考马斯亮蓝染色一产品简介：本考马斯亮蓝染色试剂盒(Commassie Blue Staining Kit)采用了最经典的考马斯亮蓝染色和脱色方法，以考马斯亮蓝R250为染料，可用于SDS-PAGE或非变性PAGE等蛋白电泳凝胶的常规染色和脱色，或Western转膜后PAGE胶上残余蛋白的检测。
使用本试剂盒，采用常规染色脱色方法累计时间达到2-3小时后可以观察到蛋白条带；采用快速染色脱色方法20分钟左右即可观察到蛋白条带。
观察到最清晰的蛋白条带则需染色脱色更长时间。
本试剂盒中的染色液和脱色液经过改良，不含有毒的甲醇，但含有刺激性气味的乙酸。
二保存条件：室温保存，至少一年有效。
注意事项：可以使用枪头盒或适当大小的培养皿作为染色和脱色的容器。
本染色液呈酸性，有轻微腐蚀性，使用时请作必要防护。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
三使用说明：1. 常规染色脱色方法：a. 电泳结束后，取凝胶放入适量考马斯亮蓝染色液中，确保染色液可以充分覆盖凝胶。
b. 置于水平摇床或侧摆摇床上缓慢摇动，室温染色1小时或更长时间。
注：具体的染色时间取决于凝胶的厚度和染色时的温度。
凝胶较厚，温度较低，则染色时间宜适当延长。
凝胶较薄，温度较高，则染色时间可以适当缩短。
通常染色至凝胶的颜色和染色液的颜色非常接近，在染色液中几乎看不清凝胶时，可以认为已染色充分。
染色2-4个小时或更长时间不会对最终的染色效果产生负面影响。
c. 倒出染色液。
染色液可以回收重复使用至少2-3次。
d. 加入适量考马斯亮蓝染色脱色液，确保脱色液可以充分覆盖凝胶。
e. 置于水平摇床或侧摆摇床上缓慢摇动，室温脱色4-24小时。
期间更换脱色液2-4次，直至蓝色背景基本上全部被脱去，并且蛋白条带染色效果达到预期。
通常蛋白条带在脱色1-2小时后即可出现。
注：脱色期间可以在脱色液中加入一片吸水纸，可以使部分染料吸附在吸水纸上，加快脱色。
脱色时间过长也会导致蛋白条带的颜色变浅。
小学下册第十四次英语第二单元测验卷(有答案)
小学下册英语第二单元测验卷(有答案)考试时间：80分钟（总分:110）A卷一、综合题(共计100题共100分)1. 听力题：The cake is _______ (layered) with cream.2. 听力题：The Berlin Wall divided _______ into East and West.3. 听力题：The chemical formula for hydrochloric acid is ______.4. 选择题：What is the capital of the USA?A. New YorkB. Washington, D.C.C. Los AngelesD. Chicago答案:B5. 填空题：My aunt is a great __________ (组织者) for family gatherings.6. 听力题：A sound that is too high or too low may not be ______.7. 听力题：The _____ of a planet determines its seasons.8. 听力题：Gases have a ______ density compared to solids and liquids.9. 听力题：The boy has a cool ________.The chemical symbol for neon is ______.11. 选择题：What do you call the shared community of plants and animals?A. EcosystemB. HabitatC. BiomeD. Population答案:A12. 听力题：Photosynthesis converts sunlight into ______ energy.13. 填空题：The _______ (The Renaissance) led to advancements in arts and sciences.14. 选择题：What do we call a baby dog?A. KittenB. PuppyC. CalfD. Chick答案:B15. 听力题：I want to learn how to ______ (cook) dishes from different countries.16. 听力填空题：I love going to science fairs to see innovative __________.17. 填空题：A hedgehog rolls into a ball when it feels ______ (害怕).18. 选择题：What is the opposite of love?A. HateB. LikeC. CareD. Enjoy答案:A19. 听力题：A ______ is a natural phenomenon that can cause destruction.What is the capital of Estonia?A. TallinnB. RigaC. VilniusD. Helsinki答案:A21. 选择题：What is the opposite of polite?A. RudeB. CourteousC. RespectfulD. Civil答案:A22. 听力题：The ______ is a giant cloud of gas and dust in space.23. 选择题：How do you say "goodbye" in Italian?A. AdiósB. CiaoC. Au revoirD. Arrivederci24. 听力题：The fire is _____ (hot/cold).25. 填空题：I enjoy _____ (writing) about plants.26. 填空题：Recognizing the signs of a healthy plant can lead to better ______ practices. (识别健康植物的迹象可以促进更好的园艺实践。
初二练习题制作希腊风味烤羊排配迷迭香英语作文
初二练习题制作希腊风味烤羊排配迷迭香英语作文Today, I would like to share with you a delightful recipe for Greekstyle roasted lamb chops with rosemary. This dish is not only flavorful but also relatively easy to prepare, making it a perfect choice for a special dinner or gathering.To begin, gather all the necessary ingredients: lamb chops, olive oil, lemon juice, garlic, rosemary, salt, and pepper. Make sure the lamb chops are at room temperature before you start cooking. Preheat your oven to 400°F (200°C) to ensure it's ready when you need it.Next, marinate the lamb chops. In a bowl, combine olive oil, lemon juice, minced garlic, chopped rosemary, salt, and pepper. Mix well to create a flavorful marinade. Coat the lamb chops with the marinade, making sure each piece is well covered. Let them marinate for at least 30 minutes to allow the flavors to penetrate the meat.Once the lamb chops have marinated, place them on a baking sheet lined with parchment paper. Make sure to space them out evenly to ensure they cook evenly. Roast the lamb chops in the preheated oven for about 2025 minutes, or until they reach your desired level of doneness. Remember to flip them halfway through the cooking process to ensure they cook evenly on both sides.While the lamb chops are roasting, you can prepare a simple side dish to complement the dish. A fresh Greek salad or roasted vegetables would be an excellent choice to serve alongside the lamb chops.Once the lamb chops are done, remove them from the oven andlet them rest for a few minutes before serving. This resting period allows the juices to redistribute, ensuring a tender and juicy final result.To serve, plate the lamb chops and garnish with a sprig of fresh rosemary for an added touch of elegance. You can also squeeze a little fresh lemon juice over the lamb chops to enhance the flavors.In conclusion, this Greekstyle roasted lamb chops with rosemary recipe is a fantastic way to incorporate Mediterranean flavors into your cooking. Whether you're hosting a dinner party or simply craving a delicious meal, this dish is sure to impress. Give it a try and enjoy the delightful combination of tenderlamb chops infused with the aromatic flavors of rosemary. Bonappétit!。
ColiComplete 产品说明书
Page 1 of 2 ColiComplete ®AOAC Official Method 992.30General DescriptionColiComplete ® contains 5-bromo-4-chloro-3-indolyl-ß-Dgalactopyranoside (X-Gal) and 4-methyl umbelliferyl-ß-D-glucuronide (MUG). Discs are added to LST inoculated with selected dilutions of samples. Samples are incubated at 35–37 °C and examined after 24 and 48 ±2 h for confirmed total coliforms and after 30 ±2 h for confirmed E. coli results. ß-Galactosidase, from coliforms present in samples, cleaves X-Gal into 5-bromo-4-chloro-indoxyl intermediate which undergoes oxidation to yield water-insoluble blue dimer, visually detectable on disc or in surrounding medium as confirmed positive result for total coliform activity. ß-Glucuronidase, from E. coli present in samples, cleaves MUG into glucuronide and methyl umbelliferone which fluoresces under long wave UV light (366 nm) as confirmed positive result for E. coli presence.NOTE : As E. coli O157:H7 does not produce ß-glucuronidase, ColiComplete ® is not suitable for the detection of E. coli O157:H7.A. Sample PreparationPrepare appropriate serial dilutions as indicated in FDA Bacteriological Analytical Manual (BAM), or AOAC Official Methods of Analysis according to sample type.B. InoculationInoculate LST tubes with appropriate sample dilution series selected to determine MPN levels or presence/absence of total coliforms and E. coli in sample. Aseptically add a single ColiComplete ® disc to each tube. Incubate at 35–37 °C.C. Reading ColiComplete ®a. For total coliforms — After at least 24 h incubation, examine each tube for visually detectable blue color on disc or in surrounding medium. Presence of blue color indicates confirmed positive result for total coliforms.NOTE: A wide range of blue color intensity may be expected, depending on sample composition and microflora. All blue reactions are positive regardless of intensity of color.Reincubate at 35–37 °C. After additional 24 ±2 h re-examine. Continued absence of blue indicates negative result; presence of blue indicates confirmed positive result for total coliforms. Read and record the MPN code or presence/absence of total coliforms in the sample.b. For E.coli — After 30 ±2 h from start of initial incubation, examine tubes under long-wave UV light (366 nm). Fluorescent tubes indicate confirmed positive result for E. coli. Read and record the MPN code or presence/absence of E. coli in the sample.D. CONTROLSPositive and negative controls should be used to facilitate interpretation of MUG fluorescent reaction. Use one known positive E. coli tube and two negative controls - one non -E. coli /coliform tube (e.g., Klebsiella spp.) and one uninoculated media tube.NOTE: Use borosilicate glass tubes, flint glass gives fluorescence that may be misinterpreted for a positive result.Lit. No. MK_UG4655EN Merck KGaAFrankfurter Strasse 25064293 DarmstadtGermanyPage 2 of 2 E. Method Modification for Certain JuicesApplicable to juice products/processors which rely on treatments that do not come into direct contact with all parts of the juice, as contained in 21 CFR Part 120: Rules and Regulations. Hazard Analysis and Critical Control Point (HAACP); Procedures for the Safe and Sanitary Processing and Importing of Juice; Final Rule. Vol 66 No. 13. 6137-6202. Use the modified method “Analysis for Escherichia coli in Citrus Juices - Modifi cation of AOAC Official Method 992.30” as stated in Section 120.25 (a).F. StorageStore unused discs at 2–8 °C (36–46 °F) in a sealed container, with desiccant.G. DisposalAfter use, all tubes must be steam-sterilized at 121 °C for at least 30 min before discarding. For in-vitro diagnostic use only.Manufacturing EntityBioControl Systems, Inc, 12822 SE 32nd St, Bellevue, WA 98005, USA.BioControl Systems, Inc is an affiliate of Merck KGaA, Darmstadt, Germany.。
考马斯亮蓝法测定(实验报告)
考马斯亮蓝法测定苹果组织微量可溶性蛋白含量吴凡郭梦雨摘要:本实验以苹果果肉为研究对象，采取考马斯亮蓝比色法测定蛋白质的吸光度值，通过对果实可溶性蛋白提取缓冲液、缓冲液浓度、pH值、外源添加物对果肉可溶性蛋白提取效率的影响的研究，优化苹果果实可溶性蛋白含量测定方法，以期优化果实可溶性蛋白测定条件，为客观反映果实可溶性蛋白水平提供一种可行的方法。
结果表明：外源添加PVP和EDTA以是苹果可溶性蛋白提取率的主要影响因素。
实验最终确定的提取条件为0.1mol/L pH9.0 Tris-HCl提取缓冲液(内含1mmol/L EDTA和1% PVP),此条件下苹果果实可溶性蛋白的有效测定含量为34.93%。
关键词：苹果、考马斯亮蓝法、Tris-HCl缓冲液、外援添加物1 实验部分1.1 实验原理果蔬可溶性蛋白质含量(soluble protein content)是一个重要的生理生化指标，也是果蔬品质和营养的重要评价指标之一。
许多可溶性蛋白是构成果蔬中酶的重要组成部分，参与果蔬多种生理生化代谢过程的调控，与果蔬的生长发育、成熟衰老，抗病性、抗逆性密切相关。
考马斯亮蓝G-250在酸性游离状态下呈棕红色，最大光吸收在465nm，当它与蛋白质结合后变为蓝色，最大光吸收在595nm。
在一定的蛋白质浓度范围内，蛋白质-染料复合物在波长为595nm处的光吸收与蛋白质含量成正比，通过测定595nm处光吸收的增加量可知与其结合蛋白质的量。
蛋白质与考马斯亮蓝G-250结合在2min左右的时间内达到平衡，完成反应十分迅速；其结合物在室温下1h 内保持稳定。
该法是1976年Bradford建立，试剂配制简单，操作简便快捷，反应非常灵敏，灵敏度高，可测定微克级蛋白质含量，测定蛋白质浓度范围为0～1 000μg/mL，最小可测2.5μg/mL蛋白质，是一种常用的微量蛋白质快速测定方法。
但该方法线性范围窄，需要选择适宜的提取缓冲液及缓冲液浓度、pH值和外源添加物等，以使测定方法有较高的灵敏度，测定值与真值相近。
BACT ALERT VIRTUO 自动血培养系统说明书
BACT/ALERT® VIRTUO®HOW FAST IS YOUR BLOOD CULTURESOLUTION?10 mLLAB INFORMATICS SOLUTIONSACCURATE Control process for reliabilityB lood Level DetectionA ct immediately when needed M onitor blood collection practices RAPIDPut time onthe patient’s sideN ew ultra-sensitive algorithmsE arlier bacterial growthdetection 1,2,3,4F AN® PLUS MEDIA for superiorantibiotic neutralization5,6AUTOMATEDStreamline work,save time & costs“Load & Go” to easily managebottles, less hands-onO ptimize your work owM anage bottles by anyone,anytime,anywhereCUSTOMIZEDFits your lab,your needsA chieve easy completecomplianceM odular & scalableE mpower your lab with MYLA®and VILINK®EMPOWERING LABS WITH FASTER,RELEVANT MICROBIOLOGY SOLUTIONSAt bioMérieux, we believe breakthrough blood culture technologies are essential forfaster, accurate results. We’ve been your partner for over 50 years with innovations bymicrobiologists, for microbiologists. BACT/ALERT® VIRTUO® is part of our integratedapproach to empower labs with solutions recognized for truly impacting better patientmanagement.12-19/93179582/GB/C/Documentand/orpicturenotlegallybinding.ModificationsbybioMérieuxcanbemadewithoutpriornotice/BIOMÉRIEUX,theBIOMÉRIEUXlogo,BACT/ALERT,FAN,FILMARRAY,MYLA,VIDAS,VILINK,VIRTUOandVITEKareused,pendingand/orregisteredtrademarksbelongingtobioMérieux,oroneofitssubsidiaries,oroneofitscompanies./B·R·A·H·M·SPCT™isthepropertyofThermoFisherScientificInc.anditssubsidiaries./Anyothernameortrademarkisthepropertyofitsrespectiveowner./bioMérieuxS.A.RCSLyon67362399/PhotosbioMérieux,N.Bouchut,C.Ganet/PrintedinFrance/théra/RCSLyonB39816REFERENCES1 - Liotti, FM. et al. “Laboratory Evaluation of the BacT/ALERT® VIRTUO™ Automated Blood Culture System”. ECCMID 2016 poster.2 - Menchinelli, F. et al,. “BacT/ALERT® VIRTUO: a New Automated Colorimetric Microbial Detection System for Detection of Bacteremia”. AMCLI 2015 Poster.3 - Mullis, D. et al. “Comparison of the bioMérieux Virtuo BacT/Alert Microbial Detection System to the BacT/Alert 3D”. Dublin 2016 Poster.4 -C han, Y-J. et al. “Comparison of the new generation of blood culture system with the BacT/ALERT®3D system for the detection of bacteremia in patients from emergency room.” ECCMID 2016 Poster.5 - D. Lovern et al. “Antimicrobial binding and growth kinetics in BacT/ALERT FA Plus and BACTEC Aerobic/F Plus blood culture bottles”, European journal of microbiology and infectious diseases, 2016; doi:10.1007/s10096-016-2759-96 - Shan L.H et al. “Evaluation study on Antimicrobial Neutralization with Automated Blood culture System sat laboratory, Sunway medical center”, MEDLAB Asia Paci c 20167 - I nternal Data- USA Performance Solutions Dept, based on 450+ customer assessments where the average time to arrival to the lab of an inoculated blood culture bottle has been equal to 10 hours, and has been reduced on average by 70%.8 - Deol P. et al. BacT/ALERT VIRTUO: Rapid time to detection di erence between the BacT/ALERT® VIRTUO™ and the BacT/ALERT® 3D 2016 : 26TH ECCMID #EV04599 - Potential pathogen ID reduction compared to VITEK 2 ID. (non binding data)10 - Compared to conventional methods.*CE-IVD marked; FDA clearedFAN® PLUS MEDIA & WORKFLOW VITEK®2VITEK®MSFILMARRAY®BACT/ALERT® VIRTUO®bioMérieux S.A. • 69280 Marcy l’Étoile • France • T el.: + 33 (0)4 78 87 20 00 • Fax: +33 (0)4 78 87 20 90 • reduced by7 hours7BLOODreduced by3.5 hours8reduced by23 hours9reduced by1 day10reduced by5 hours9ANTIBIOTICMANAGEMENT:VIDAS®B R A H M S PCT™SEPSISSUSPICION:VIDAS®。
考马斯亮蓝溶液配制方法
考马斯亮蓝溶液配制方法嘿，朋友们！今天咱就来讲讲考马斯亮蓝溶液的配制方法。
这可是个挺重要的事儿呢！先来说说需要准备的东西哈，那就是考马斯亮蓝粉末啦，还有乙醇、磷酸等一些试剂。
就好像做饭得有食材一样，这些就是我们配制溶液的“食材”哟！咱先拿个合适的容器，就像个小碗似的。
然后把适量的考马斯亮蓝粉末放进去，这粉末看着不起眼，可作用大着呢！接着呢，再慢慢地把乙醇倒进去。
哎呀，你可别一下子倒太多啦，得一点点来，就像给花浇水似的，轻轻柔柔的。
然后呢，就开始搅拌啦，要把它们搅拌均匀，让粉末完全溶解在乙醇里，这过程就像是给面团揉匀一样。
等搅拌得差不多了，再加入磷酸等其他试剂。
这一步可不能马虎，加多少得把握好，就跟做菜放盐一样，多了咸少了淡。
加完后继续搅拌搅拌，让它们充分融合在一起。
你说这考马斯亮蓝溶液就像是一个小团队，各种成分就是团队里的成员，只有大家齐心协力，才能发挥出最大的作用呀！在配制的过程中，可得仔细着点，别粗心大意的。
要是不小心弄错了什么，那可就前功尽弃啦！就像盖房子，一块砖没放好，可能整座房子都不结实呢。
等溶液配制好了，就可以拿去用啦。
可以用它来做各种实验，看看蛋白质的含量啥的。
这就像是给我们的科学研究打开了一扇门，让我们能看到更多的奥秘。
你想想，要是没有这正确配制的考马斯亮蓝溶液，那些实验该怎么做呀？那不就像战士上战场没带武器一样嘛！所以说，这配制方法可得好好掌握。
朋友们，都记住了吗？其实配制考马斯亮蓝溶液也不难，只要用心去做，肯定能成功的。
就像我们生活中的很多事情一样，只要肯下功夫，就没有做不到的。
加油吧，让我们一起在科学的海洋里畅游，用这神奇的考马斯亮蓝溶液去探索更多的未知！。
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运输与保存方法
室温运输和保存，有效期一年。
注意事项
1） 为了您的安全和健康，请穿实验服并带一次性手套操作。 2） 用本产品 4 分钟即可显示出蛋白条带，加长染色时间和增加染色次数，有利于提高染色灵敏度。 3） 如果凝胶面积增大或者厚度增加，增加染色液和染色时间。 4） 切忌长时间高功率加热，否则会使溶液连续沸腾蒸发，造成凝胶干裂。如果不慎使凝胶干裂，可加入 50ml 去离子水，
上海翊圣生物科技有限公司 客服热线：4006-111-883 E-mail: order@
Commassie Blue Fast Stain Solution (8 mins)
考马斯亮蓝快速染色液（8 分钟）
产品信息
产品名称 Commassie Blue Fast Stain Solution (8 mins) 考马斯亮蓝快速染色液（8 分钟） Commassie Blue Fast Stain Solution (8 mins) 考马斯亮蓝快速染色液（8 分钟）
本品是基于考马斯亮蓝染色法对蛋白质进行染色而设计的，与传统染色液相比，本品为新型 PAGE 胶染色试剂，不含甲 醇，醋酸等有毒或有刺激性物质，其独特配方使得表面活性剂剥离，蛋白固定，蛋白染色，非蛋白区域着色屏蔽等功能同时 进行。8-10 分钟即可完成 page 胶上蛋白染色，无需脱色。染色分辨率与传统染色方法分辨率相当。染色的蛋白带或蛋白点 可进行高效电洗脱，适用于蛋白质谱分析。
产品编号 20309ES03 20309ES08
规格 1L 5L
储存 室温 室温
产品描述
实验室常使用考马斯亮蓝染色法对凝胶上分离的蛋白进行染色，它既克服了氨基黑染色灵敏度不高的限制，又比银染简 便易操作。目前广泛用的考马斯亮蓝有 G250 和 R250 两种。其中考马斯亮蓝 G250 由于与蛋白质的结合反应十分迅速，常 用于蛋白质含量的测定。考马斯亮蓝 R250 与蛋白质反应虽然比较缓慢，但是可以被洗脱下去，所以可以用来对电泳条带染 色。
1）剥下电泳完毕的SDS-PAGE，或native-PAGE胶，（约10×10cm，厚度小于1mm），放在比凝胶稍大的可微波炉加热的带 盖塑料盒中（注意：一定要有盖以防止水汽蒸发过度）；
2） 加入50ml染色液，放入微波炉里先用高火（微波炉最大“火”）加热1分钟，取出染胶盒放在水平摇床上，中速震荡3-5min。 倒掉染色液。
高火加热 1min，低火加热 3-5min，可一定程度恢复干裂的 PAGE 胶，但染色效果有一定毁坏。
操作步骤
1. 染色工作液的配制
本品是以 125×浓缩液的形式提供的，每瓶 8ml。 使用前只需要取一瓶 8ml 的染色原液用去离子水或者蒸馏水稀释到 1L，混匀后即为染色工作液，室温保存即可。
2. 染色步骤