保护碱基表
2 hr
20 hr
G GTCGAC C 00CG GTCGAC CG 00CCG GTCGAC CGG 00C ACATGT G 00CC ACATGT GG >90>90CCC ACATGT GGG >90>90GGCGCGCC >90>90A GGCGCGCC T >90>90TT GGCGCGCC AA >90>90C CCCGGG G 50>90CC CCCGGG GG >90>90TCC CCCGGG GGA >90>90C GGATCC G 1025CG GGATCC CG >90>90CGC GGATCC GCG >90>90C AGATCT G 00GA AGATCT TC 75>90GGA AGATCT TCC 25>90G GCGCGC C 00AG GCGCGC CT 00TTG GCGCGC CAA 50>90BstE II G GGT(A/T)ACC C
010AACTGCAGAA CCAATGCATTGG 00AAAACTGCAG CCAATGCATTGG AA 2550CTGCAGAA CCAATGCATTGG ATGCAT
25>90C ATCGAT G 00G ATCGAT C 00CC ATCGAT GG >90>90CCC ATCGAT GGG 5050G GAATTC C >90>90CG GAATTC CG >90>90CCG GAATTC CGG >90>90GG GGCC CC >90>90AGC GGCC GCT >90>90TTGC GGCC GCAA >90>90C AAGCTT G 00CC AAGCTT GG 00CCC AAGCTT GGG 1075G GGTACC C 00GG GGTACC CC >90>90CGG GGTACC CCG >90>90G ACGCGT C 00CG ACGCGT CG 2550C CCATGG G
切割率%
Acc I
Afl III
Asc I
Ava I
BamH I
酶寡核苷酸序列
EcoR I
Hae III
Hind III
Kpn I
Bgl II
BssH II
BstX I
Cla I
Mlu I Nco I
CATG CCATGG CATG
5075C CATATG G 00CC CATATG GG 00CGC CATATG GCG
00GGGTTT CATATG AAACCC 00GGAATTC CATATG GAATTCC 75>90GGGAATTC CATATG GAATTCCC
75>90G GCTAGC C 00CG GCTAGC CG 1025CTA GCTAGC TAG
10
50
2 hr
20 hr
TT GCGGCCGC AA 00ATTT GCGGCCGC TTTA 1010AAATAT GCGGCCGC TATAAA 1010ATAAGAAT GCGGCCGC TAAACTAT 2590AAGGAAAAAA GCGGCCGC AAAAGGAAAA
25>90TGC ATGCAT GCA
10>90CCA ATGCAT TGGTTCTGCAGTT
>90>90TTAATTAA 00G TTAATTAA C 025CC TTAATTAA GG
0>90GTTTAAAC 00G GTTTAAAC C 025GG GTTTAAAC CC
050AGCTTT GTTTAAAC GGCGCGCCGG 75>90G CTGCAG C
00TGCA CTGCAG TGCA
1010AA CTGCAG AACCAATGCATTGG >90>90AAAA CTGCAG CCAATGCATTGGAA >90>90CTGCAG AACCAATGCATTGGATGCAT
00C CGATCG G 00AT CGATCG AT 1025TCG CGATCG CGA 010Sac I C GAGCTC G 1010G CCGCGG C 00TCC CCGCGG GGA
50>90GTCGAC GTCAAAAGGCCATAGCGGCCGC 00GC GTCGAC GTCTTGGCCATAGCGGCCGCGG 1050ACGC GTCGAC GTCGGCCATAGCGGCCGCGGAA
1075G AGTACT C 1025AAA AGTACT TTT 7575CCCGGG 010C CCCGGG G 010CC CCCGGG GG 1050TCC CCCGGG GGA
>90
>90
酶寡核苷酸序列
切割率%Not I
Nco I
Nde I
Nhe I
Pvu I
Sac II Sal I
Sca I Nsi I Pac I
Pme I
Pst I
Sma I
G ACTAGT C 10>90GG ACTAGT CC 10>90CGG ACTAGT CCG 050CTAG ACTAGT CTAG 050G GCATGC C 00CAT GCATGC ATG 025ACAT GCATGC ATGT 1050A AGGCCT T >90>90GA AGGCCT TC >90>90AAA AGGCCT TTT >90>90C TCTAGA G 00GC TCTAGA GC >90>90TGC TCTAGA GCA 75>90CTAG TCTAGA CTAG 75>90C CTCGAG G 00CC CTCGAG GG 1025CCG CTCGAG CGG 1075C CCCGGG G 00CC CCCGGG GG 2575CCC CCCGGG GGG 50>90TCCC CCCGGG GGGA
>90
>90
Xba I
Xho I
Xma I
Spe I
Sph I
Stu I