脉冲电磁场磁疗与骨硬化蛋白单克隆抗体联合干预绝经后新西兰大白兔骨代谢和骨微结构的变化
低频脉冲电磁场治疗原发性骨质疏松症的临床观察
低频脉冲电磁场治疗原发性骨质疏松症的临床观察秦茵;李玲;张元菊【期刊名称】《中国骨与关节杂志》【年(卷),期】2007(006)006【摘要】目的观察低频脉冲电磁场对原发性骨质疏松症患者的治疗作用.方法对60例原发性骨质疏松症患者进行低频脉冲电磁场治疗,每次40min,1次/d,每周治疗5次,共24周为1个疗程,观察其治疗作用.结果治疗后在48例有疼痛症状的患者中,疼痛消失或缓解43例(P＜0.05),总有效率为89.6%.血清骨钙素水平提高5.8%.6个月后复查显示平均腰椎(L2-4)骨密度提高0.56%,平均股骨颈骨密度提高1.38%,平均大转子骨密度提高了1.29%,平均髋部Wards三角区骨密度提高1.87%(P均＜0.05).治疗过程中及治疗后无1例出现不良反应.治疗前后患者血常规、肝肾功能、血钙、血磷均在正常范围内.结论 PEMFs能缓解原发性骨质疏松症患者疼痛症状,改善骨代谢以及提高骨密度.【总页数】3页(P348-350)【作者】秦茵;李玲;张元菊【作者单位】350025,福州,南京军区福州总医院中医理疗科;解放军总医院第一附属医院康复理疗科;解放军总医院第一附属医院康复理疗科【正文语种】中文【中图分类】R6【相关文献】1.低频脉冲电磁场治疗原发性骨质疏松症的实验研究进展 [J], 夏璐;何成奇2.补肾益骨膏联合低频脉冲电磁场治疗老年原发性骨质疏松症临床观察 [J], 黄明炜;廖勇敢;李艳丽;陈思思;汪茜3.低频脉冲电磁场联合鲑鱼降钙素治疗原发性骨质疏松症 [J], 张捷;陈永茂;沈朝东4.低频脉冲电磁场治疗原发性骨质疏松症的临床观察 [J], 秦茵5.运用低频脉冲电磁场治疗原发性骨质疏松症 [J], 李小平因版权原因，仅展示原文概要，查看原文内容请购买。
脉冲电磁场合用钙剂治疗绝经后骨质疏松症的疗效观察
脉冲电磁场合用钙剂治疗绝经后骨质疏松症的疗效观察
王玉英;李素梅;吴鸣
【期刊名称】《中国临床保健杂志》
【年(卷),期】2004(007)001
【摘要】目的对脉冲电磁场(PEMFs)治疗绝经后骨质疏松症疗效进行观察和探讨.方法确诊为绝经后骨质疏松症20例,采用磁振热机器(选用其主要的脉冲电磁场成分)加口服钙剂治疗,每次1 h,共30次,治疗50 d及治疗后6个月观察其自觉症状改善情况及骨密度(BMD)的变化,并与治疗前对比分析.结果 100%患者疼痛明显减轻,治疗50 d结束时平均BMD绝对增长0.048 g/cm2,治疗后6个月达0.083
g/cm2.结论 PEMFs治疗绝经后骨质疏松症可以有效控制疼痛,提高患者的生存质量,对患者BMD也有一定程度的提高.
【总页数】2页(P25-26)
【作者】王玉英;李素梅;吴鸣
【作者单位】安徽省立医院,康复医学科,合肥,230001;安徽省立医院,内分泌科,合肥,230001;安徽省立医院,康复医学科,合肥,230001
【正文语种】中文
【中图分类】R454.1;R591.44
【相关文献】
1.低频脉冲电磁场治疗绝经后骨质疏松症的临床疗效观察 [J], 姜俊良;梁邱;杨浩伦;何成奇;杨霖
2.雌激素加钙剂与钙剂对比治疗绝经后妇女骨质疏松症的骨密度测定分析 [J], 张宏红;吴春维;何秀华
3.雌激素辅以钙剂治疗绝经后骨质疏松症疗效观察 [J], 董利;郭厉华
4.雌激素、乐力钙联合用药治疗绝经后骨质疏松症 [J], 张玉霞;尹玉香
5.淫羊藿总黄酮联合钙剂和维生素D治疗绝经后骨质疏松症的临床疗效观察 [J], 金雨
因版权原因，仅展示原文概要，查看原文内容请购买。
脉冲电磁场对骨质疏松的生物效应_关志成
T he prev entive ex periments demonstrate that the co rrected bone mineral density is hig her than that o f O VX g roup (P <0. 05). I n the further studies, the serum bo ne marker and biomechanics pro per ties w ill be te sted. Based o n the efficacy of cellular level and animal mo del, the effects o f pulsed electro magnetic fields w ere inv estiga ted , the bone mineral density w as improved and the pain level was alleviated significantly after treatment. Consequently , it is concluded
松的防治中发挥重要作用 。
关键词 :脉冲电磁场 ;骨质疏松 ;机理 ;成骨细胞 ;动物模型 ;临床研究
脉冲电磁场治疗绝经后骨质疏松症的Meta分析
脉冲电磁场治疗绝经后骨质疏松症的Meta分析徐换;郝赤子;郑俊;廖维靖【摘要】Objective: To systematically review the effectiveness and safety of pulsed electromagnetic fields (PEMFs) in treating patients with postmenopausal osteoporosis (PMO), so as to provide a basis for clinical treatment and further study.Method: The randomized controlled trials (RCTs) on PEMFs in treating patients with PMO were searched on China National Knowledge Internet (CNKI), Wanfang Data medical information system (WF), VIP information resource system (VIP), Chinese Biomedical Literature Database (CBM), PubMed, The Cochrane Library and Web of Science from inception to August 2016.Two reviewers independently screened articles according to the inclusion and exclusion criteria, extracted data and assessed the risk of bias of included studies.Then meta-analysis was performed using RevMan 5.3 software.Results: A total of 12 RCTs involving 1134 patients were included.The results of meta-analysis showed that there was statistically significant difference in VAS (SMD=-2.07, 95% CI-2.74 to-1.40, P<0.01) between the two groups, but there was no statistically significant difference in BMD of the lumbar spine(SMD=0.79, 95% CI 0.00 to 1.57, P=0.05) and femoral neck (SMD=0.77, 95% CI-0.04 to 1.57, P=0.06), and the adverse events (RR=0.33, 95% CI 0.09 to 1.20, P=0.09) between the two groups.Conclusion: Actual clinical evidence indicates that PEMFs are effective and safe for PMO to a certainrge-sample, multi-center and high-quality studies are still neededfor validation because of the limitation of the quantity and quality of the included studies.%目的:系统评价脉冲电磁场(PEMFs)治疗绝经后骨质疏松症(PMO)的有效性和安全性,为临床治疗和进一步的研究提供参考依据.方法:计算机检索中国知网(CNKI)、万方数据库、维普数据库(VIP)、中国生物医学文献数据库(CBM)、PubMed、The Cochrane Library和Web of Science数据库,搜集有关PEMFs治疗PMO患者的随机对照试验(RCT),检索时限为从建库至2016年8月.由2名研究者按照纳入与排除标准独立筛选文献、提取资料并评价纳入研究的偏倚风险后,采用RevMan 5.3软件进行Meta分析.结果:共纳入12个RCT,合计患者1134例.Meta分析结果显示,VAS评分[SMD=-2.07,95%CI(-2.74,-1.40),P<0.01]2组间差异具有统计学意义,腰椎BMD[SMD=0.79,95%CI(0.00,1.57),P=0.05]、股骨颈BMD[SMD=0.77,95%CI(-0.04,1.57),P=0.06]、不良反应率[RR=0.33,95%CI(0.09,1.20),P=0.09]方面,2组间差异均无统计学意义.结论:现有临床证据表明,PEMFs对PMO的治疗有一定疗效,安全性较好,但受纳入研究数量和质量限制,尚需大样本、多中心、高质量的研究加以验证.【期刊名称】《中国康复》【年(卷),期】2017(032)003【总页数】5页(P230-234)【关键词】脉冲电磁场;绝经后骨质疏松症;系统评价;Meta分析;随机对照试验【作者】徐换;郝赤子;郑俊;廖维靖【作者单位】武汉大学中南医院康复医学科,武汉 430071;武汉大学中南医院康复医学科,武汉 430071;武汉大学中南医院康复医学科,武汉 430071;武汉大学中南医院康复医学科,武汉 430071【正文语种】中文【中图分类】R49;R681早期有效预防和治疗骨质疏松症(Osteoporosis，OP)对提高绝经后妇女生活质量、改善其预后、减轻家庭及社会精神压力及经济负担有重要意义[1]。
脉冲电磁场对老年大鼠关节软骨及软骨下骨凋亡蛋白的影响
脉冲电磁场对老年大鼠关节软骨及软骨下骨凋亡蛋白的影响李萌萌;尹林伟;牛尚博;刘静;黄夏荣;王金玲;孙光华;钟培瑞;彭婷;曾国锋;周君【期刊名称】《风湿病与关节炎》【年(卷),期】2024(13)5【摘要】目的:探讨脉冲电磁场对老年大鼠关节软骨及软骨下骨凋亡蛋白的影响。
方法:采用随机抽样法将16只24月龄SD自然衰老雄性大鼠随机等分为老年组和脉冲电磁组,8只6月龄SD青年雄性大鼠作为青年组。
脉冲电磁组予脉冲电磁场干预,频率为8 Hz,强度为3.82 mT,每日40 min,每周5 d,持续8周。
青年组和老年组固定于脉冲电磁场下,仪器不通电。
8周后取材,ELISA法检测血清Ⅱ型胶原C 端肽(CTX-Ⅱ)含量;显微CT检测左侧胫骨软骨下骨微结构并分析骨组织形态量化指标;番红O-固绿染色观察大鼠左侧胫骨平台软骨组织形态结构,改良Mankin's评分评估大鼠膝关节软骨组织退行性变化程度。
RT-qPCR、Western blot检测半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)、半胱氨酸天冬氨酸蛋白酶-8(Caspase-8)、血小板反应蛋白解整合素金属蛋白酶-4(ADAMTS-4),以及血小板反应蛋白解整合素金属蛋白酶-5(ADAMTS-5)的mRNA与蛋白表达水平。
结果:①与青年组比较,老年组大鼠软骨胶原分解产物CTX-Ⅱ水平升高(P<0.05);骨密度、骨体积分数、骨小梁数量均降低(P<0.05),骨小梁厚度、骨小梁间距均增大(P<0.05);番红O-固绿染色显示,大鼠软骨表面粗糙有裂隙,软骨与软骨下骨界限模糊不清,且有潮线,低细胞化,细胞形态结构不规则,蛋白多糖丢失且红染变淡;改良Mankin's评分增加(P<0.05);Caspase-3、Caspase-8、ADAMTS-4、ADAMTS-5的mRNA及蛋白表达升高(P<0.05)。
②与老年组比较,脉冲电磁组大鼠软骨胶原分解产物CTX-Ⅱ水平降低(P<0.05);左侧胫骨软骨下骨的骨密度、骨体积分数升高,骨小梁数量增加(P<0.05),骨小梁间距减小(P<0.05),骨小梁厚度有降低趋势(P>0.05);番红O-固绿染色显示,大鼠软骨表面较为平整,软骨与软骨下骨界限分明,细胞形态较规则、数量较多,蛋白多糖丰富且染色均匀;改良Mankin's评分较小(P<0.05);Caspase-3、Caspase-8、ADAMTS-4、ADAMTS-5的mRNA及蛋白表达降低(P<0.05)。
低频脉冲电磁场联合阿仑膦酸钠治疗绝经后骨质疏松骨密度及骨代谢的影响
低频脉冲电磁场联合阿仑膦酸钠治疗绝经后骨质疏松骨密度及骨代谢的影响郑黎勤 郑衍庆广东省佛山市中医院三水医院，广东佛山 528100[摘要] 目的 观察低频脉冲电磁场联合阿仑膦酸钠治疗绝经后骨质疏松的疗效和安全性。
方法 将78例绝经后骨质疏松患者按随机数字表法分为试验组和对照组，对照组患者给予阿仑膦酸钠治疗，试验组在对照组的基础上加用低频脉冲电磁场，比较两组患者治疗效果。
结果 治疗4个月后，两组患者股骨颈、股骨粗隆、Wards三角和腰椎BMD水平，血清TRACP-5b、BAP、BGP水平以及VAS评分均较治疗前改善（P＜0.05），试验组改善幅度大于对照组（P＜0.05）；试验组有效率明显高于对照组（P＜0.05）；两组不良反应发生率比较无统计学意义（P＞0.05）。
结论 低频脉冲电磁场联合阿仑膦酸钠治疗绝经后骨质疏松效果肯定，安全性较好。
[关键词] 低频脉冲电磁场；阿仑膦酸钠；绝经后骨质疏松[中图分类号] R580 [文献标识码] A [文章编号] 2095-0616（2018）07-247-04 Effects of low frequency pulsed electromagnetic fields combined with alendronate on bone mineral density and bone metabolism in postmenopausal osteoporosisZHENG Liqin ZHENG YanqingSanshui Hospital,Foshan Hospital of Traditional Chinese Medicine,Guangdong province,Foshan 528100,China [Abstract] Objective To observe the efficacy and safety of low frequency pulsed electromagnetic fields combined with alendronate in the treatment of postmenopausal osteoporosis. Methods Seventy-eight cases of postmenopausal osteoporosis were randomly divided into the experimental group and the control group.The patients in the control group were treated with alendronate,while the experimental group was added low frequency pulsed electromagnetic fields on the basis of the control group.The therapeutic effects of two groups were compared. Results After 4 months of treatment,the levels of femoral neck,trochanter,Wards triangle and lumbar BMD,the serum levels of TRACP-5b,BAP,BGP and VAS scores in the two groups were better than before treatment (P＜0.05).The improvement of the experimental group was greater than that of the control group (P＜0.05).The effective rate of the experimental group was significantly higher than that of the control group (P＜0.05).There was no significant difference in the incidence of adverse reactions between the two groups (P＞0.05). Conclusion Low frequency pulsed electromagnetic field combined with alendronate sodium in the treatment of postmenopausal osteoporosis is effective and safe.[Key words] Low frequency pulsed electromagnetic field;Alendronate sodium;Postmenopausal osteoporosis骨质疏松是一种老年人易患的全身代谢性骨病，以严重的全身疼痛为主要表现，并严重影响患者的生活质量，造成极大的不便。
两种干预周期脉冲电磁场对原发性骨质疏松症的疗效比较
两种干预周期脉冲电磁场对原发性骨质疏松症的疗效比较田永芝;田发明;张柳;程爱国;梁春雨【期刊名称】《中国骨质疏松杂志》【年(卷),期】2015(21)1【摘要】Objective To compare the efficacy of different treatment periods with pulse electromagnetic fields ( PEMFs ) on primary osteoporosis. Methods A total of 124 cases of osteoporosis in our hospital from October 2010 to January 2012 were enrolled in this study. They were divided into whole period group and half period group. The patients in whole period group ( 62 cases) received 60 times PEMFs treatment, with 6⁃12Hz frequency and 9⁃11mT intensity, using a TY⁃PEMF A2 ( Tong⁃Ye Company, Tianjin) . Each treatment lasted for 40 minutes. The total course was 5 months. The patients in the half period treatment group received 30 times treatment. The total course was two and half months. All patients received calcium carbonate and vitamin D 300mg twice a day, alfacalcidol 1 ug once a day, and alendronate sodium pill 70 mg once a week. The bone mineral density of the total hip, femoral neck, and lumbar vertebral ( L2⁃L4 ) was detected before and after the treatment. Results The BMD of the total hip, femoral neck, and lumbar vertebral L2⁃L4 in both groups markedly increased after the treatment comparing to that before the treatment. The increasing rates of BMD in whole period group and half period group were 8�31 ± 1�01 and 5�45 ± 1�17 in the total hip, 8�58 ± 1�40 and 5�44± 1�52 in the femoral neck, and 8�02 ± 0�63 and 4�97 ± 0�49 in the lumbar vertebrae, respectively. The difference among them was statistically significant. Conclusion PEMFs combined with anti⁃osteoporosis drugs can increase the BMD of osteoporosis patients. The whole period of PEMFs has better efficacy than half period treatment.%目的：比较脉冲电磁场（ pulsed electric⁃magnetic fields， PEMFs）在原发性骨质疏松症骨重建周期全程干预和半程干预的疗效。
低频脉冲磁场对兔膝关节软骨细胞人白介素1β、肿瘤坏死因子α表达与分泌的影响
低频脉冲磁场对兔膝关节软骨细胞人白介素1β、肿瘤坏死因子α表达与分泌的影响刘明晖;窦逾常;晋学飞;丁保冬【期刊名称】《中国医药导报》【年(卷),期】2012(9)29【摘要】目的探讨低频脉冲磁场对兔膝关节软骨细胞人白介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)表达与分泌的影响.方法选择40只健康成年新西兰白兔建立兔膝关节软骨损伤模型,把右膝关节设为治疗组(n = 20),每日给予低频脉冲磁场治疗;左膝关节为对照组(n = 20),给予假磁场治疗,治疗后1、4周深麻醉处死动物,观察相关指标的变化.结果在同一观察时间点,IL-1β和TNF-α表达量治疗组均明显少于对照组,差异均有统计学意义(均P < 0.05).治疗组软骨损伤程度较对照组轻微,关节活动度明显大于对照组,outerbridge积分也少于对照组,差异均有统计学意义(均P < 0.05).结论低频脉冲磁场治疗骨性关节炎具有简便易行,安全无创等优点,可有效遏制IL-1β、TNF-α的释放,调节分泌作用,从而对骨性关节炎软骨损伤修复具有促进作用.%Objective To investigate the effects of expression and secretion of human interleukin 1(3 (TL 1 (5), tumor necrosis factor α (TNF-α) in rabbit articular cartilage cells by the low-frequency pulsed magnetic field. Methods 40 healthy adult New Zealand white rabbits were selected to construct the articular cartilage injury model, the right knees were chosen as the treatment group (n - 20) and given daily low-frequency pulsed magnetic field therapy; the left knees in - 20) were chosen as the control group and given false magnetic field therapy, after the treatment of 1 and4 weeks, the rabbits were given deep anesthesia and sacrificed to observe the changes of the relevant indicators. Results A.E ihe same time points, the expression of IL-1β and TNF-α in the treatment gro up were lower than those in control group, the differences were all statistically significant (all P ＜ 0.05). The degree of cartilage damage in treatment group was less than that in control group, the outerbridge points in treatment group were less than that in control group, the joint activity of the treatment group were better than that of the control group, the differences were all statistically significant (all P ＜ 0.05). Conclusion The low-frequency pulse magnetic field therapy for treating osteoarthritis is simple, safe and non-invasive which can effectively curb the release and regulate the secretion of IL-1β and TNF-α, thus it can promote the repair of the osieoarthritis cartilage injury.【总页数】3页(P32-33,36)【作者】刘明晖;窦逾常;晋学飞;丁保冬【作者单位】吉林大学中日联谊医院中医科,吉林长春,130033;吉林大学中日联谊医院中医科,吉林长春,130033;吉林大学中日联谊医院泌尿外科,吉林长春,130033;吉林大学中日联谊医院急诊科,吉林长春,130033【正文语种】中文【中图分类】R683【相关文献】1.体外冲击波对兔膝关节炎软骨细胞丝裂原活化蛋白激酶表达的影响 [J], 刘洪柏;侯晓东2.丹参注射液对骨性关节炎模型兔膝关节软骨细胞p-IκBα表达影响的实验研究[J], 徐西林;张晓峰;吕航;夏联恒;李小东;宿慧;刘沛然;王顺3.硫酸氨基葡萄糖胶囊对兔膝关节软骨细胞凋亡基因Bcl-2与Bax表达的影响 [J], 林宗汉;郑铁牛;黎强;覃学流;容向宾;龙飞攀;梁庆华;王永乐4.低频脉冲磁场对兔膝关节软骨细胞白介素-1β、肿瘤坏死因子-α表达与分泌的影响 [J], 张志刚;曹成;王根林;朱雪松;耿德春;施勤;毛海青;邹俊5.伸筋易骨矫形手法对兔膝关节软骨细胞代谢及Sox9表达的影响 [J], 赵强;王一洲因版权原因，仅展示原文概要，查看原文内容请购买。
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Pulsedbined with sclerostin antibody improves bone metabolism and bone microstructure in postmenopausal osteoporosis rabbits
钱光，男，1979 年生，河 南省睢县人，回族，2005 年同济大学毕业，硕士， 副主任医师，主要从事脊 柱外科和组织工程研究。
王明海，博士，主任医师， 副教授，复旦大学附属上 海市第五人民医院骨科， 上海市 200240
文献标识码:A 投稿日期：2019-08-31 送审日期：2019-09-02 采用日期：2019-10-15 在线日期：2020-01-04
Corresponding author: Wang Minghai, MD, Chief physician, Associate professor, Department of Orthopedics, Shanghai Fifth People’s Hospital, Fudan University, Shanghai 200240, China
(1)卵巢切除组； (2)脉冲电磁场组(PEMF 组)；
(3)骨硬化蛋白单克隆抗体组(Scl-Ab 组)； (4) 脉 冲 电 磁 场 + 骨 硬 化 蛋 白 单 克 隆 抗 体
组(PEMF+ Scl-Ab 组)。
干预 8 周后检测： (1)骨代谢指标； (2)骨密度； (3)骨微结构。
文题释义： 频脉冲电磁场(pulsed electromagnetic fields，PEMFs)疗法：是目前常用的骨质疏松症物理治疗方法。脉 冲电磁场治疗骨质疏松症的原理是采用低频脉冲电磁场改变人体生物电、改善生物场，促使成骨细胞增生， 增强成骨能力，提高骨密度治疗骨质疏松。 骨硬化蛋白的单克隆抗体(sclerostin antibody，Scl-Ab)：对骨代谢的影响集中体现在对骨硬化蛋白的拮抗 作用上。骨硬化蛋白单克隆抗体在刺激成骨活动的同时，不会刺激破骨活动，对骨合成代谢有显著刺激作 用，其已经成为治疗骨质疏松的潜在方法。
Abstract BACKGROUND: Both pulsed electromagnetic fields (PEMF) stimulation and sclerostin antibody (Scl-Ab) have good effects on the bone metabolism of ovariectomized (OVX) New Zealand rabbits, but research on the combined intervention of PEMF and Scl-Ab in the OVX rabbits is rarely reported. OBJECTIVE: To explore the effect of PEMF combined with Scl-Ab on postmenopausal osteoporosis and to explore the therapeutic value for osteoporosis. METHODS: An animal model of postmenopausal osteoporosis was made in New Zealand white rabbits after ovariectomy. The experimental animals were randomly divided into OVX control group, PEMF group, Scl-Ab group and PEMF+Scl-Ab group, with 10 rats in each group. On the 1st day after surgery, the PEMF group was given PEMF magnetic therapy once a day; the Scl-Ab group was given subcutaneous injection of Scl-Ab twice a week; the PEMF+Scl-Ab group received PEMF magnetic therapy once a day, five times a week, and Scl-Ab subcutaneous injections twice a week; the OVX group was injected subcutaneously with the same dose of normal saline twice a week for 8 weeks. After 8 weeks of treatment, bone metabolism index, bone mineral density, and MicroCT bone microstructure parameters were detected. All animal procedures were approved by the Department of Experimental Animal Science, Fudan University (approval No. 20171263A193). RESULTS AND CONCLUSION: Bone mineral density was significantly decreased in the New Zealand white rabbits after 6 months of OVX, suggesting that the osteoporosis model was successfully established. Compared with the OVX group, the bone mineral density of the L3 vertebral body in the PEMF group, the Scl-Ab group and the PEMF+Scl-Ab group increased significantly (P < 0.05). Compared with the OVX group, serum bone-specific alkaline phosphatase levels were significantly higher, and serum tartrate-resistant acid phosphatase 5b levels were significantly lower in the PEMF group, the Scl-Ab group and the PEMF+Scl-Ab group. The serum tartrate-resistant acid phosphatase 5b level in the PEMF+Scl-Ab group was significantly lower than that in the PEMF group and the Scl-Ab group. The bone metabolism index and bone microstructural parameters (bone volume fraction, trabecular thickness, trabecular number, and trabecular separation) of the PEMF+Scl-Ab group were significantly better than those of the PEMF group and the Scl-Ab group (all P < 0.05). These findings indicate that the combination of Scl-Ab and PEMF can enhance bone mineral density and improve bone metabolism and bone microstructure in postmenopausal osteoporosis New Zealand white rabbits. Key words: pulsed electromagnetic field; sclerostin antibody; combination treatment; osteoporosis; bone mass; bone mineral density; rabbit Funding: the Natural Science Foundation of Minhang District of Shanghai, No. 2018MHZ038 (to QG)
摘要 背景：脉冲电磁场与骨硬化蛋白单克隆抗体皆能对绝经后新西兰大白兔骨代谢产生良好影响，但关于两者 联合干预的效果至今少有报道。 目的：探讨脉冲电磁场联合骨硬化蛋白单克隆抗体对绝经后新西兰大白兔骨代谢的影响，探索其对骨质疏 松症的治疗价值。 方法：采用卵巢切除法建立新西兰大白兔绝经后骨质疏松动物模型。将实验动物随机分为 4 组：卵巢切除 组、脉冲电磁场组、骨硬化蛋白单克隆抗体组和脉冲电磁场+骨硬化蛋白单克隆抗体组，每组 10 只。术后 第 1 天起，脉冲电磁场组给予脉冲电磁场磁疗每天 1 次；骨硬化蛋白单克隆抗体组给予骨硬化蛋白单克隆 抗体皮下注射每周 2 次；脉冲电磁场+骨硬化蛋白单克隆抗体组接受脉冲电磁场磁疗每天 1 次、每周 5 次， 骨硬化蛋白单克隆抗体皮下注射每周 2 次；卵巢切除组皮下注射相同剂量的生理盐水每周 2 次，干预共 8 周。8 周后行骨代谢指标检查、骨密度测定、MicroCT 骨微结构参数检测。动物研究中的所有程序经复旦 大学实验动物科学部批准(20171263A193)。 结果与结论：①卵巢切除 6 个月新西兰大白兔骨密度显著下降，提示骨质疏松模型建立成功；②与卵巢切 除组相比，3 个治疗组 L3 椎体的骨密度均显著增加(P < 0.05)；③3 个治疗组血清骨特异性碱性磷酸酶水平 均显著高于卵巢切除组，血清抗酒石酸酸性磷酸酶 5b 水平均显著低于卵巢切除组；脉冲电磁场+骨硬化蛋 白单克隆抗体组血清抗酒石酸酸性磷酸酶 5b 的水平明显低于脉冲电磁场组、骨硬化蛋白单克隆抗体组；④ 脉冲电磁场+骨硬化蛋白单克隆抗体组骨微结构参数(骨体积分数､ 骨小梁厚度､ 骨小梁数量､ 骨小梁分离 度)均优于脉冲电磁场组、骨硬化蛋白单克隆抗体组(均 P < 0.05)；⑤骨硬化蛋白单克隆抗体和脉冲电磁场 联合治疗可以增强去势新西兰大白兔骨密度，改善骨代谢和骨微结构。 关键词： 脉冲电磁场；骨硬化蛋白单克隆抗体；联合治疗；骨质疏松症；骨量；骨密度；兔 中图分类号：R446；R496；R318 基金资助： 上海市闵行区自然科学基金项目资助(2018MHZ038)，项目负责人：钱光 缩略语：