aatcc30-2017防霉检测标准
防霉抗菌测试标准及机构介绍[5篇范例]
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防霉抗菌测试标准及机构介绍[5篇范例]第一篇：防霉抗菌测试标准及机构介绍防霉抗菌测试标准及机构介绍标准分为：国际标准（ISO），国家标准（GB，JIS，ASTM），行业标准（QB、HG、JC等），地方标准（DB），企业标准（Q/L）等。
SGS(瑞士）ITS（英国）BV（法国）TUV（德国）纺织品：防霉：AATCC30-2010 织物抗真菌性的评价-抑制织物的霉变（美国标准）JIS Z 2911-2010 纺织品耐霉菌试验方法（日本标准）GB/T 24346-2009 纺织品防霉性能的评价（国家标准）FZ/T 60030-2009 家用纺织品防霉性能测试方法抗菌：AATCC 135 耐水洗AATCC 100-2012后整理抗菌织物的抗细菌性评价（美国标准）AATCC 147-2011织物的抗细菌性评价（美国标准）JIS L 1902-2008 纺织品抗菌性试验法（日本）ISO 20743-2007 纺织品抗菌性试验方法（全球通用）GB/T 20944-2007纺织品抗菌性能评价 FZ/T 73023-2006 抗菌针织品QB/T 2881-2007鞋类衬里和内垫材料抗菌技术条件 FZ/T 62015 抗菌毛巾塑料防霉：QB/T 2591 抗菌塑料-抗菌性能试验方法和抗菌效果GB/T 24128-2009 塑料防霉性能试验方法ISO 846-1997塑料在微生物作用下的行为评价抗菌：AATM E 2149 JISZ 2801 QB/T 2591 抗菌塑料-抗菌性能试验方法和抗菌效果JC/T 939-2004 建筑用抗细菌塑料管抗细菌性能ISO 22196-2007 塑料制品表面抗菌性能评价涂料防霉：HG/T 3950-2007 抗菌涂料GB/T 1741-2007 漆膜耐霉菌测定法抗菌：HG/T 3950-2007 抗菌涂料GB/T 21866-2008 抗菌涂料（漆膜）抗菌性测定法和抗菌效果其他防霉：ASTM G 21-2009 合成聚合材料防霉性的测定ASTM D 4576-2008 蓝色原料（皮革）抗霉菌生长的试验方法GB/T 2423-16-2008 电子电工产品环境试验抗菌：ASTM E 2149-2001 在动态接触条件下固定抗菌剂抗菌活性测定的标准试验方法 JIS Z 2801-2010 抗菌加工制品-抗菌性试验方法和抗菌效果 GB /T 21510-2008 纳米无机材料抗菌性能检测方法 JC/T 897-2014 抗菌陶瓷制品抗菌性能测试时间防霉：一般28天，但有特殊的，如AATCC 30，可以7天，或者14天抗菌：一般情况下，做个测试时间是3-4天，但是检测机构是7-10工作日一般所做的菌种为：大肠杆菌，金黄色葡萄球菌，白色念珠菌。
AATCC30试验方法
AATCC试验方法30-1993 美国国家标准纺织材料上抗真菌活性的测定:防霉防腐AATCC RA 31 委员会1946年制订; 1952,1957,1971,1981,1987,1988(改题),1993年修订1970,1974,1979,1989年确认;1986年编辑修订与确认.-------------------------------------------------------------------------1 目的和范围1.1 本试验方法目的有二,即测定纺织材料对霉与腐的敏感性及评价纺织材料上杀菌剂效能。
2. 原理2.1 单独或混合使用试验I,II,III,IV,取决于所试纺织材料暴露形式,例如, 最终产品是与土壤接触的,则使用相似于该暴露的试验I;如整理产品从不与土壤或热带条件相接触的,则使用稍温和的试验(即试验II或III).试验II是专为含纤素纤维的材料设计的.而试验III则为其他材料.如所有材料均使用于室外土地上的则须用方法IV.当评价纺织材料有关真菌的生长时要考虑二个条件:(1)纺织产品实际的败坏(腐化)和(2)对产品并不败坏,但形成看不见的(霉),常带有不愉快的霉味.2.2 当最终用途要求苛刻(见附录A),可对产品作某些预暴露.当最终用途近高温,杀菌剂可能挥发,应作烘箱预暴露.当最终用途在热带或淋雨户外时,则在霉菌试验前须作有关淋雨的预试验。
可能时，纺织材料在试验前均需作予期条件的予试验。
3 术语3.1 防霉,名词--暴露在有利于真菌生长条件的纺织材料上,对看不见的真菌成长并伴有不愉快霉味发展的防止.3.2 防腐，名词－使纺织材料腐败的真菌生长的防止。
注：这些腐败通常以测断裂强度评定。
4 安全予防注：这些安全予防仅作为资料性的。
列入试验程序中的予防措施并不全面。
使用者有责任用安全专门技术来掌握本方法中的材料。
制造厂应提供规格细节诸如安全数据单和其他建议。
所有OSHA标准和规则也必须咨询和遵守。
国内外常见抗菌防霉标准
中国
卫生部《消毒技术规范》-2002版
国家技术法规
在卫生部系统普遍采用。是消毒剂/卫生用品的试验方法标准。
GBT 20944.1-2007纺织品抗菌性能的评价第1部分：琼脂扩散法GBT 20944.2-2007纺织品抗菌性能的评价第2部分：吸收法
纺织品，抗细菌
是ISO 20743/日本JIS L 1902标准的等同采用。
ISO 846-1978（E）塑料在真菌和细菌作用下的行为的测试--用直观检验法或用测量质量或物性变化的方法评价
塑料，抗细菌/防霉
是用来检测霉菌作用对塑料的破坏效果的，但试验方法可用于防霉检测。
2
中国
QB/T 2591-2003抗菌塑料——抗菌性能评价及其测试方法
塑料，抗细菌/防霉
是我国影响很大的抗菌材料抗菌测试标准，被广泛用于坚硬固体表面抗菌性能的评价，是ISO 21996/日本JIS Z 2801标准非等同采用的标准。
国内外常见抗菌防霉标准
序号
国家和组织
标准号和标准名称
适用
范围
备注
1
国际标准化组织
ISO 22196-2007塑料制品表面抗菌性能评价方法Plastics Measurement of antibacterial
塑料，
抗细菌
是日本JIS Z 2801标准和中国QB/T 2591标准的对应国际方法标准。
ASTM G21-96（2002）Standard Practice for Determining Resistance of Synthetic Polymeric Materials to Fungi合成聚合材料防霉（耐真菌）性能测试标准
合成聚合物，防霉
美国材料试验协会标准
AATCC测试方法
AATCC测试方法AATCC测试方法标准编号委员会标准名称AATCC 6-2006 RRl 耐酸和耐碱色牢度AATCC 8-2007 RA38 耐摩擦色牢度：AATCC摩擦测试仪法AATCC 15-2007 RR52 耐汗渍色牢度AATCC 16-2004 RA50 耐光色牢度AATCC 17-2005 RA63 润湿剂效果的评价AATCC 20-2007 RA24 纤维分析：定性AATCC 20A-2007 RA24 纤维分析：定量AATCC 22-2005 RA63 拒水性：喷淋试验AATCC 23-2005 RA33 耐烟熏色牢度AATCC 26-2004 RR9 硫化染料染色纺织品的老化测试：快速法AATCC 27-2004 RA63 润湿剂：再润湿剂的评估方法AATCC 28-2004 RA49 纺织品的防虫害AATCC 30-2004 RA31 抗菌性：纺织品防霉和防腐性能AATCC 35-2006 RA63 拒水性：淋雨测试AATCC 42-2007 RA63 拒水性：冲击渗水性测试AATCC 43-2004 RA63 丝光润湿剂的测试方法AATCC 61-2007 RA60 耐洗涤色牢度：快速法AATCC 66-2003 RA61 机织物折皱回复性的测定：回复角法AATCC 70-2005 RA63 拒水性：滚筒罐动态吸水性测试AATCC 76-2005 RA32 织物表面电阻率AATCC 79-2007 RA63 纺织品的吸水性AATCC 81一2006 RA34 湿处理纺织品水萃取液pH：值的测定AATCC 82-2007 RA34 漂白棉布的纤维素分散质流度的测定AATCC 84-2005 RA32 纱线的电阻AATCC 86-2005 RA43 印花图案及整理剂的干洗耐久性AATCC 88B-2006 RA61 织物经多次家庭洗涤后的缝线平整度AATCC 88C-2006 RA61 织物经多次家庭洗涤后的褶裥保持性AATCC 89-2003 RA66 棉花丝光AATCC 92-2004 RR35 残留氯强力损失：单试样法AATCC 93-2005 RA29 织物的耐磨性能：埃克西来罗测试仪法AATCC 94-2007 RR45 纺织品整理剂：鉴别方法AATCC 96-2004 RA42 机织物和针织物(除毛织物外)商业洗涤后的尺寸变化AATCC 97-1999 RA34 坯布及前处理后纺织品中的可萃取物含量AATCC 98-2007 RA34 过氧化氢漂白浴中碱含量的测定AATCC 99-2004 RA42 机织或针织毛纺织品尺寸变化：松弛、毡合和毡缩AATCC 100-2004 RA31 纺织材料抗菌整理剂的评定AATCC 101-2004 RA34 耐过氧化氢漂白色牢度AATCC 102-2007 RA34 高锰酸钾滴定法测定过氧化氢AATCC 103-2004 RA34 退浆中使用的细菌仅一淀粉酶的分析AATCC 104-2004 RA23 耐水斑色牢度AATCC 106-2007 RA23 耐水色牢度：海水AATCC 107-2007 RA23 耐水色牢度AATCC 109-2005 RA23 耐低湿大气中臭氧色牢度AATCC 110-2005 RA36 纺织品的白度AATCC 111-2003 RA64 纺织品耐气候性：日光和气候曝晒AATCC 112-2003 RR68 织物释放甲醛含量的测定：密封广口瓶法AATCC 114-2005 RR35 残留氯强力损失：多试样法AATCC 115-2005 RA32 织物静电吸附：织物与金属测试AATCC 116-2005 RA38 耐摩擦色牢度：旋转垂直摩擦仪法AATCC 117-2004 RR54 耐干热色牢度(热压除外)AATCC 118-2007 RA56 拒油性：抗碳氢化合物测试AATCC119-2004 RA29 平磨变色(霜白)：金属丝网法AATCC 120-2004 RA29 平磨变色(霜白)：金刚砂法AATCC 121-2005 RA57 地毯沾污：视觉评级法AATCC 122-2000 RA57 地毯沾污：使用沾污法AATCC 123-2000 RA57 地毯沾污：快速沾污法AATCC 124-2006 RA61 织物经多次家庭洗涤后的外观平整度AATCC 125-2004 RA50 耐汗光色牢度AATCC 127-2003 RA63 抗水性：静水压法AATCC 128-2004 RA61 织物折皱回复性：外观法AATCC 129-2005 RA33 耐高湿大气中臭氧色牢度AATCC 130-2000 RA56 去污性：油渍清除法AATCC 131-2005 RR53 耐褶裥色牢度：蒸汽褶裥AATCC 132-2004 RA43 耐干洗色牢度AATCC 133-2004 RR54 耐热色牢度：热压AATCC 134-2006 RA32 地毯的静电倾向AATCC 135-2004 RA42 织物经家庭洗涤后的尺寸稳定性AATCC 136-2003 RA79 粘合和层压织物的粘合强度AATCC 137-2007 RA57 小地毯背面对乙烯地板的玷污AATCC 138-2005 RA57 去污：纺织地毯的洗涤AATCC 139-2005 RA50 耐光色牢度：光致变色的测定AATCC 140-2006 RA87 染料和颜料在浸轧烘干过程中的泳移性评价AATCC 141-2004 RA87 用于腈纶的碱性染料的配伍性AATCC 142-2005 RR8l 植绒织物多次家庭洗涤和(或)投币式干洗后的外观AATCC 143-2006 RA61 服装及其他纺织制品经多次家庭洗涤后的外观AATCC 144-2007 RA34 纺织品湿加工过程中的总碱含量AATCC 146-2006 RA87 分散染料的分散性：过滤测试法AATCC 147-2004 RA3l 纺织品的抗菌性：平行划线法AATCC 149-2007 RA90 螯合剂：氨基多元酸及其盐类的螯合值测定--草酸钙法AATCC 150-2003 RA42 服装经家庭洗涤后的尺寸稳定性AATCC 151-2003 RA56 抗污物再沉积：洗涤仪法AATCC 154-2006 RA87 分散染料的热固色性能AATCC 157-2005 RR92 耐溶剂斑色牢度：全氯乙烯AATCC 158-2005 RA43 全氯乙烯干洗的尺寸变化：机洗法AATCC 159-2006 RA87 酸性染料和酸性媒介染料在锦纶上的移染AATCC 161-2007 RA90 螯合剂：由金属引起的分散染料色变及对比AATCC 162-2002 RA23 耐水色牢度：氯化游泳池水AATCC 163-2007 RR92 色牢度：储存中的染料转移织物到织物AATCC 164-2006 RA33 耐高湿大气中二氧化氮色牢度AATCC 165-1999 RA57 耐摩擦色牢度：铺地纺织品--AATCC摩擦测试仪法AATCC 167-2003 RA87 分散染料的起泡性AATCC 168-2007 RA90 螯合剂：聚氨基多元羧酸及其盐类活性成分含量分析：潘酚(PAN)铜法AATCC 169-2003 RA64 纺织品的耐气候性：氙弧灯曝晒AATCC 170-2006 RA87 粉末状染料粉尘化倾向的评定AATCC 171-2005 RA57 地毯去污：热水萃取法AATCC 172-2007 RA60 家庭洗涤中耐无氯漂白粉色牢度AATCC 173-2005 RA36 CMC：可接受的小色差计算AATCC 174-2007 RA31 地毯抗微生物活性的测定AATCC 175-2003 RA57 抗沾色性：毛绒地毯AATCC 176-2006 RA87 染料分散液色斑现象的评定AATCC 178-2004 RR97 横档的视觉评定和评级AATCC 179-2004 RA42 经家庭洗涤的织物纬斜和成衣扭曲性能AATCC 181-2005 RA50 高温耐光色牢度：可控日光温度和湿度仪器法AATCC 182-2005 RA36 染料在溶液中的相对着色力AATCC 183-2004 RAl06 紫外辐射通过织物的透过或阻挡性能AATCC 184-2005 RA87 染料粉尘化特性的测试AATCC 185-2006 RA90 螯合剂：过氧化氢漂白浴中螯合剂的百分含量：潘酚(PAN)铜指示剂法AATCC 186-2006 RA64 纺织品耐气候性：紫外光和湿态曝晒AATCC 187-2004 RA42 织物尺寸稳定性：快速法AATCC 188-2003 RA60 耐次氯酸钠家庭漂白洗涤色牢度AATCC 189-2007 RA57 地毯纤维的含氟量AATCC 190一2003 RA60 耐活性氧漂白剂家庭洗涤色牢度：快速法AATCC 191-2004 RA41 酸性纤维素酶对纤维素的影响测定：上装式洗衣机法AATCC 192-2005 RA64 纺织品耐气候性：给湿与不给湿条件下日弧灯曝晒AATCC 193-2007 RA56 拒水性：抗水/乙醇溶液测试AATCC 194-2007 RA49 纺织品在长期测试条件下抗室内尘螨性能的评定AATCC评定程序EP 1-2007 RA36 变色灰卡EP 2-2007 RA36 沾色灰卡EP 4-2007 RA36 标准深度卡EP 5-2006 RA89 织物手感：主观评定EP 6-2003 RA36 仪器测色方法EP 7-2003 RA36 仪器评定试样的变色EP 8-2007 RA36 AATCC沾色彩卡EP 9-2007 RA36 纺织品色差的视觉评价EP 10-2007 RA59 多纤维贴衬织物的评定EP 11-2007 RA36 用荧光增白纺织品校准分光光度计uV能量的程序。
纺织品抗菌性能测试方法及标准
纺织品抗菌性能测试方法及标准抗菌纺织品的最重要的性能指标是抗菌性。
测试抗菌性时，要求培养基浓度、温湿度、pH 值及试验时间与穿衣条件相一致，实验仪器应为微生物实验常用仪器，且对任何形状的纺织材料都能测试[1]。
抗菌性的测试方法中，发展较早的是日本和美国，最有代表性且应用较广的是美国的AATCC试验法100和日本的工业标准。
国内使用较多的评价方法一般都是参照AATCC (American Association of Textile Chemists and Colorists，美国纺织染色家和化学家协会)标准[2]和日本JAFET(日本纤维制品新功能协议会)批准的"SEK"标志认证标准的方法[3]。
我国于1992年颁布了纺织行业标准FZ/T01021-1992《织物抗菌性能试验方法》[4]，1996年颁布了国家标准GB15979-2002《一次性使用卫生用品卫生标准》[5]。
但是抗菌性能评价的方法和标准还远末作到系统、统一、规范，尤其是抗菌纺织品的性能评价和产品规范在我国还有许多问题不明确，只能做到简单的定性检测。
鉴于当前我国对抗菌纺织品的全面评价还不能适应国内生产和对外贸易的需要，本文对目前世界上使用较多的抗菌测试方法及标准进行了对比，1 测试菌种的选择微生物(microorganism)是存在于自然界的一群体形细小、结构简单、肉眼无法直接看到，必须借助显微镜等设备才能观察到的微小生物。
绝大多数的微生物对人类和动植物是无害的，甚至是有益和必需的。
但是也有小部分的微生物可以引起人类和动植物的病害[6]。
因而人们在进行抗菌性能的评价中，菌种的选择必须具有科学性和代表性。
表1列出的菌种是在自然界和人体皮肤及粘膜上分布最为广泛的。
测试的菌种[7]包括细菌和真菌。
在细菌中主要用革兰氏阳性菌(金黄色葡葡球菌、巨大芽胞杆菌、枯草杆菌)和革兰氏阴性菌(大肠杆菌、荧光假单胞杆菌)；在真菌中主要用霉菌(黑曲霉、黄曲霉、变色曲霉、桔青霉、绿色木霉、球毛壳霉、宛氏拟青霉、腊叶芽枝霉)和癣菌(石膏样毛癣菌、红色癣菌、紫色癣菌、铁锈色小抱子菌、袍子丝菌、白色念珠菌)。
浅析纺织品防霉性能测试标准和方法比较
浅析纺织品防霉性能测试标准和方法比较沈小敏;余晓娜【摘要】防霉性能是功能性纺织品的重要性能.本文比较了国内外纺织品防霉性能的测试标准,即GB/T 24346-2009、FZ/T 60030-2009、AATCC 30-2017、AATCC 174-2011、BS EN 14119-2003、JIS Z 2911-2010,从标准的适用范围、测试菌种、结果评价等方面,分析纺织品防霉测试标准的现状,比较了各个测试方法的差异,讨论了现有标准体系存在的一些问题,为防霉纺织品的检测工作提供一些参考.【期刊名称】《中国纤检》【年(卷),期】2019(000)007【总页数】3页(P102-104)【关键词】纺织品;防霉;标准【作者】沈小敏;余晓娜【作者单位】广州检验检测认证集团有限公司;广州检验检测认证集团有限公司【正文语种】中文1 引言功能性纺织品是指除一般纺织品所具有的基本使用价值外，还具有某种（些）特殊功能的新型纺织品。
随着功能性纺织品的研发不断进展，纺织品的各种功能性能在消费者的生活中体现越来越多的价值。
功能性纺织品可以大致分为：防护功能纺织品（防紫外线性能、阻燃性能、抗静电性能、电磁屏蔽性能、拒水性能、拒油性能等）、舒适功能纺织品（如吸湿发热性能、吸湿排汗性、透湿性、透气性、保暖性等）、卫生保健纺织品（如抗菌、防霉、防螨、防虫蛀性能、远红外保健性能等）、易护理功能纺织品（如防污性能、易去污性、免烫性能等）。
其中，防霉性能和我们的日常生活联系颇为紧密，霉菌无孔不入、无处不在，只要有合适的环境，它就会大量地繁殖，特别是在潮湿温暖的地方，很多物品上会长出一些肉眼可见的绒毛状、絮状或蜘蛛网状的菌落。
每年三四月份，特别是我国的南方，雨水较多，气温渐升，家里的墙壁、衣物、毛巾等易出现起霉菌，发出难闻的异味，对我们的生活和健康造成严重的影响。
2 纺织品防霉性能的测试方法纺织品防霉性能是指产品具有抑制霉菌孢子萌发及菌丝体在纺织品上面生长繁殖的能力。
AATCC标准测试完整版
AATCC标准测试完整版干洗色牢度-AATCC 1321.仪器及材料1.1 水洗色牢度实验机1.2不锈钢洗杯:直径为75mm～高为125mm～容量为500ml。
1.3 不锈钢珠1.4 未染色斜纹棉布:克重为270+70g/m2～未经整理过的～切割成尺寸为120mm x120mm的布片1.5 全氯乙烯,干洗级1.6 AATCC变色灰卡～AATCC沾色灰卡1.7 分光光度计1.8 摩擦附布1.9 洗涤剂～Perk-Sheen2.测试样品2.1 如果被测试物为织物～每种样布取3块样品～每块样品尺寸为10x5cm。
长边方向平行于经向或纬向。
2.2 如果被测试物为纱线～将其织成尺寸为10x 5cm的织片～长边方向平行于经向或纬向。
2.3 样品准备2.3.13.测试程序3.1 将两块未染色的正方形棉斜纹布沿三边缝合～制成一个内尺寸为100mmx100mm的布袋～将一块试样和12片不锈钢圆片放入袋中～用任何方便的形式缝住袋口。
3.2 把装有试样和钢片的布袋放在容器内～在30C+2加入200ml全氯乙烯～如用其它溶剂～需在报告中说明。
在规定的装臵中～30C+2C处理试样30min。
3.3 从溶剂中拿出布袋～取出试样～夹于吸水袋或布之间～挤压或离心去除多余的溶剂～将试样悬挂在温度为60+5的热空气中烘干。
3.4 用灰卡评定试样的变色3.5 实验结束后～用滤纸过滤留在容器中的溶剂。
将过滤后的溶剂和空白溶剂到入臵于白卡前的比色管～采用透射光～用评定沾色用灰卡比较两者的染色。
汗渍色牢度-AATCC151(设备～材料和试剂1.1 AATCC 汗渍实验器或等同装臵,带有塑料～玻璃压片,1.2 烘箱—对流式1.3 天平～精确度到0.0011.4 多纤维附布NO.10或FA1.5 pH计～精确度到0.01。
1.6 5步或9步AATCCA彩色沾色灰卡或沾色灰卡。
1.7 变色灰卡1.8 轧车1.9 白色吸水纸1 / 231.10 酸性人工汗液。
高清版 AATCC 30-2013(L2)
98TM 30-2013AATCC Technical Manual/2014Developed in 1946 by AATCC Commit-tee RA31; revised 1952, 1957, 1971,1981, 1987, 1988 (with title change),1993, 1999; reaffirmed 1970, 1974,1979, 1989, 1998, 2013; editorially re-vised and reaffirmed 1986, 2004; edito-rially revised 2010.1. General Purpose and Scope1.1 The two purposes of this test method are to determine the susceptibil-ity of textile materials to mildew and rot and to evaluate the efficacy of fungicides on textile materials.2. Principle2.1 Tests I, II, III and IV can be used,singly or in combination, depending on the type of exposure to which the textile material will be subjected. For example,if the final product will come in contact with soil, Test I, which simulates this ex-posure, should be used; if the finished product will never come in contact with soil or tropical conditions, a much less severe test (e.g., II or III) should be used.Test II is specifically designed for cellu-lose-containing materials while Test III is for all others. For all materials intended for outdoor and above ground use, Test IV should be used. The two important considerations when evaluating textile materials in relation to fungal growth are (1) the actual deterioration of the textile product (rot), and (2) growth not neces-sarily deteriorating the product but mak-ing it unsightly (mildewy) often with an unpleasant and musty odor.2.2 Certain pre-exposures of textile products may be indicated when specific end-uses are critical (see Appendix A).When the end-use will be near high tem-perature and the fungicide may be vola-tile, a preliminary oven exposure may be desired. When the end-use will be in tropical exposures or outside with rainfall present, a leaching exposure should be performed before mildew evaluation is made. When at all possible, the textile material should be exposed to the ex-pected conditions of use prior to perform-ing this test.3. Terminology3.1 mildew resistance, n.—in textiles ,resistance to development of unsightly fungal growths and accompanying un-pleasant, musty odors on textile materialsexposed to conditions favoring such growths.3.2 rot resistance, n.—in textiles , re-sistance to deterioration of a textile mate-rial as a result of fungal growth in or on it.NOTE: Such deterioration is normally assessed by measuring loss in tensile strength.4. Safety PrecautionsNOTE: These safety precautions are for information purposes only. The pre-cautions are ancillary to the testing proce-dures and are not intended to be all inclu-sive. It is the user’s responsibility to use safe and proper techniques in handling materials in this test method. Manufac-turers MUST be consulted for specific details such as material safety data sheets and other manufacturer’s recommenda-tions. All OSHA standards and rules must also be consulted and followed.4.1 This test should be performed only by trained personnel. The U.S. Depart-ment of Health and Human Services pub-lication Biosafety in Microbiological and Biomedical Laboratories should be con-sulted (see 24.1).4.2 CAUTION: Some of the fungi used in these tests are allergenic and patho-genic; i.e., capable of infecting humans and producing disease. Therefore, every necessary and reasonable precaution must be taken to eliminate this risk to the laboratory personnel and to personnel in the associated environment. Wear protec-tive clothing, respiratory protection, and impervious gloves when working with the organisms. NOTE: Choose respira-tory protection that prevents penetration by the spores.4.3 Good laboratory practices should be followed. Wear safety glasses in all laboratory areas.4.4 All chemicals should be handled with care.4.5 An eyewash/safety shower should be located nearby for emergency use.4.6 Sterilize all contaminated samples and test materials prior to disposal.4.7 Exposure to chemicals used in this procedure must be controlled at or below levels set by government authorities (e.g.,Occupational Safety and Health Administration’s [OSHA] permissible ex-posure limits [PEL] as found in 29 CFR 1910.1000; see web site: for latest version). In addition, the Ameri-can Conference of Governmental Indus-trial Hygienists (ACGIH) ThresholdLimit Values (TLVs) comprised of time weighted averages (TLV-TWA), short term exposure limits (TLV-STEL) and ceiling limits (TLV-C) are recommended as a general guide for air contaminant ex-posure which should be met (see 24.2).Test I Soil Burial5. Scope5.1 This procedure is generally consid-ered to be the most severe test for textile products. Only those specimens that will come in direct contact with soil—such as sandbags, tarpaulins, tents—need to be tested by this procedure. It can also be used for testing experimental textile fungicides.6. Test Specimens6.1 Prepare the fabric specimens with dimensions 15.0 ± 1.0 × 4.0 ± 0.5 cm (6.0± 0.4 × 1.5 ± 0.2 in.) with the long dimen-sion parallel to the warp and unraveling to 2.5 ± 0.1 cm width (1.0 ± 0.04 in.), or,in the case of fabric with less than 20threads per 2.5 cm (1.0 in.) to a predeter-mined number of threads to give a speci-men 2.5 ± 1.0 cm in width (1.0 ± 0.4 in.).A sample cutter can also be used (see 24.3). The number of specimens will vary according to the number of variables. The suggested number of specimens is five for each treatment, control and reference fabric.7. Test Procedure7.1 Viability control: Expose untreated cotton cloth 271 g/m 2 (8 oz/yd 2) in the soil bed for seven days during the test pe-riod to verify fungal activity. The soil bed shall be considered as satisfactory if the viability control fabric loses 90% break-ing strength after seven days exposure.7.2 Soil Bed: Place the air-dry test soil (see 24.4) in trays, boxes or suitable con-tainers to a depth of 13.0 ± 1.0 cm (5.1 ±0.4 in.) and bring to optimum moisture content by gradual addition of water ac-companied by mixing to avoid puddling.After allowing it to stand for 24 h, sieve it through a 6.4 mm (0.25 in.) mesh screen.Maintain uniform moisture content by covering the soil container with a suitable lid. The moisture content of the soil dur-ing the test period shall be maintained be-tween 25 ± 5% (based on dry weight). IfAATCC Test Method 30-2013Antifungal Activity, Assessment on Textile Materials:Mildew and Rot Resistance of Textile MaterialsCopyright © 2013 American Association of Textile Chemists and ColoristsCopyright The American Association of Textile Chemists and Colorists Provided by IHS under license with AATCC--`,,```,,,,````-`-`,,`,,`,`,,`---the surrounding air is maintained at higher than 83 ± 3% relative humidity,the loss of moisture is negligible.7.3 Incubation: Bury the specimens horizontally on 10.0 ± 1.0 cm (3.9 ±0.4in.) of soil, spaced at least 2.5 cm (1.0 in.) apart and then cover with 2.5 ±0.5 cm (1.0 ± 0.2 in.) of test soil. Incuba-tion periods can vary from 2-16 weeks,depending on severity of service require-ments, and other factors of importance to the interested parties. Maintain the tem-perature at 28 ± 1°C (82 ± 2°F) during the test period.8. Evaluation and Report8.1 Strength loss determination: Re-move specimens, gently wash with water,dry at room temperature for 22 ± 4 h and then condition in an atmosphere of 64 ±2% humidity and a temperature of 24 ±3°C (76 ± 6°F) for 24 h. Determine break-ing strength by the method outlined in ASTM D5035, Standard Test Method for Breaking Force and Elongation of Textile Fabrics (Strip Force), using 25 × 75 mm (1× 3 in.) jaw faces. The gage length is de-termined as 25% of the specimen length.Testing can be performed every two weeks or as specified by the end-user.8.2 Report: Report the length of expo-sure to the soil bed, percent retained breaking strength when compared to the unexposed textile, any pre-exposure of specimens before burying and percent re-tained breaking strength of untreated specimen and/or viability control.Test IIAgar Plate, Chaetomium Globosum 9. Scope9.1 This procedure is used for evaluat-ing rot resistance of cellulose-containing textile materials that will not come in contact with soil. It may also be used for determining uniformity of fungicide treatment.10. Test Specimens10.1Proceed as in Section 6, if strength loss is to be determined. If only a visual examination is performed, a mini-mum of five samples is required. How-ever, any number can be tested depending on end-users request. Cut 3.8 ± 0.5 cm (1.5 ± 0.2 in.) diameter discs from both treated and untreated samples.11. Test Procedure11.1 Organism: Chaetomium globo-sum . American Type Culture Collection No. 6205 (see 24.5).11.2 Culture medium (see 24.6): The mineral salts agar should have the follow-ing composition:Ammonium nitrate, NH 4NO 3........3.0 gPotassium dihydrogen phosphate,KH 2PO 4....................................2.5 g Dipotassium hydrogen phosphate,K 2HPO 4....................................2.0 g Magnesium sulfate,MgSO 4 · 7H 2O..........................0.2 g Ferrous sulfate,FeSO 4 · 7H 2O...........................0.1 g Agar............................................20.0 g Distilled water....................to 1000 mL 11.3 Inoculum: Place agar solution in any desired container such as test tube,French square bottle, Erlenmeyer flask, or petri dish. Sterilize in an autoclave at 103kPa (15 psi) and 121°C (250°F) for 15min and cool in a position which affords maximum inoculation surface. After the agar has hardened, under aseptic condi-tions, place on its surface a disc of filter paper previously sterilized by dry heat in an oven at 71 ± 3°C (160 ± 5°F) for 1 h.Streak the filter paper with spores of Cha-etomium globosum by use of a sterile nee-dle. Incubate at 28 ± 1°C (82 ± 2°F) for approximately 10-14 days to produce abundant growth. Remove the filter paper from the container and add it to 50 ± 2mL of sterile distilled water containing a few glass beads and shake vigorously to bring the spores into suspension. Use this suspension for inoculum in 11.5.11.4 Culture chamber: Melt mineral salts agar of the composition specified in 11.2 in an autoclave and distribute into any convenient container. Sterilize under conditions given in 11.3 and leave undis-turbed until the agar hardens.11.5 Inoculation: Pre-wet the speci-mens (but do not rub or squeeze) in water containing 0.05% of a nonionic wetting agent (see 24.7) and place in contact with the hardened agar medium in each con-tainer under aseptic conditions. Distribute 1.0 ± 0.1 mL of the inoculum evenly over the 15.0 ± 1.0 × 4.0 ± 0.5 cm (6.0 ± 0.4 ×1.5 ± 0.2 in.) specimens by means of a sterile pipette. Use 0.2 ± 0.01 mL of inoc-ulum for the 3.8 ± 0.5 cm (1.5 ± 0.2 in.)discs. Set up a control specimen, cellu-lose filter paper or untreated control, in a similar way by using 1.0 ± 0.1 or 0.2 ±0.01 mL of sterile water.12. Evaluation and Report12.1 Strength loss evaluation: Proceed as per 8.1 and report the change in break-ing strength as compared to the sample before exposure or the control if available.12.2 Visual assessment: Report the ex-tent of fungal growth on the discs, using a microscope (50X) where necessary, in accordance with the following scheme:Observed GrowthNo growthMicroscopic growth (visible only un-der the microscope)Macroscopic growth (visible to the eye)Test IIIAgar Plate, Aspergillus Niger 13. Scope13.1 Certain fungi, of which Aspergil-lus niger is one, can grow on textile prod-ucts without causing measurable break-ing strength loss within a laboratory experimental time frame. Nonetheless,their growth may produce undesirable and unsightly effects. This procedure is used to evaluate textile specimens where growth of these fungi is important.14. Test Specimens14.1 Cut duplicate 3.8 ± 0.5 cm (1.5 ±0.2 in.) diameter discs from both treated and untreated samples. Other shapes and sizes can be used provided any antici-pated size of the growth-free zone is taken into consideration.15. Test Procedure15.1 Organism: Aspergillus niger ,American Type Culture Collection No.6275 (see 24.5).15.2 Culture medium: Proceed as per 11.2. Other suitable media are Czapek (Dox) Agar and Sabouraud Dextrose Agar (see 24.8).15.3 Inoculum: Add scrapings from a ripe (7-14 days) fruiting culture of As-pergillus niger grown on the medium de-scribed in 11.2 containing 3.0 ± 0.1% glu-cose, to a sterile Erlenmeyer flask containing 50 ± 1 mL of sterile water and a few glass beads. Shake the flask thor-oughly to bring the spores into suspen-sion. Use this suspension as the inoculum.15.4 Inoculation: If the test medium contains glucose, distribute evenly 1.0 ±0.1 mL of the inoculum over the surface of the agar. Pre-wet the discs (but do not rub or squeeze) in water containing 0.05% of a nonionic wetting agent (see 24.7) and place on the agar surface. Dis-tribute evenly over each disc 0.2 ± 0.01mL of the inoculum by means of a sterile pipette. If the test medium does not con-tain glucose, a negative control fabric is required to ensure inoculum viability. In-cubate all specimens at a temperature of 28 ± 1°C (82 ± 2°F) for 14 days when mineral salts agar is used and for seven days when 3% glucose is added to the mineral salts agar.16. Evaluation and Report16.1 At the end of the incubation pe-riod, report the percentage of surface area of the discs covered with the growth of Aspergillus niger , using a microscope (50X) where necessary, in accordance with the following scheme:--`,,```,,,,````-`-`,,`,,`,`,,`---100TM 30-2013AATCC Technical Manual/2014Observed GrowthNo growth (if present, report the size of the growth-free zone in mm)Microscopic growth (visible only un-der the microscope)Macroscopic growth (visible to the eye)Test IVHumidity Jar, Mixed Spore Suspension 17. Scope17.1 This test method is designed todetermine the fungistatic effectiveness of treatments intended to control mildew and non-pathogenic fungal growth on ar-ticles or surfaces composed of textile ma-terials intended for outdoor and above ground use and which are usually water-proofed.17.2 For this test method visual assess-ment is used. Additionally, breaking strength may be determined by method as per 8.1.18. Principle18.1 Treated and untreated, nutrient-saturated strips of fabric are sprayed with a mixed spore suspension of mildew-causing organisms and incubated at 90 ±2% relative humidity. Mildew growth on treated and untreated strips is rated at weekly intervals for up to four weeks.19. Apparatus19.1 Glassware: 500 mL French square jars or equivalent with fitting screw caps.Caps are modified by center drilling and inserting an appropriate size stainless steel or brass bolt to which a hook (formed from a 6.5 ± 0.5 cm length [2.6 ±0.2 in.] of #22 nickel-chromium wire or other non-corrosive wire) is attached.19.2 Plastic paper clips or nylon thread to suspend the specimens from the screw caps of the French jars.19.3 Atomizer, DeVilbiss #152 (or equivalent) operated at 69 kPa (10 psi).19.4 Counting chamber suitable for de-termining spore concentrations; e.g.,hemocytometer.20. Test Specimens20.1 The specimens are prepared by cut-ting 2.5 ± 0.5 cm × 7.5 ± 0.5 cm (1.0 ± 0.2× 3.0 ± 0.2 in.) strips from sample weigh-ing 170.0 ± 34.0 g/m 2 (5.0 ± 1.0 oz/yd 2).For heavier fabrics use strips 2.0 ± 0.5 cm × 2.0 ± 0.5 cm (0.8 ± 0.2 × 0.8 ± 0.2 in.).20.2 Use at least four specimens of each treated and untreated fabric.20.3 Untreated fabric strips, identical in all other respects to the treated speci-mens under test, are required to establish the test validity. If untreated fabrics arenot available, use a control cloth with the following requirements:Cotton:American type, good mid-dling Warp:18.5 tex z 886 × 2S748Weft:30 tex z 630 × 2S748Weave:Plain 34 ends/cm and 17picks/cmMass/unit area:230.0 g/m 2 (6.8 oz/yd 2)Finish:Scoured only21. Test Procedures21.1 Organisms:21.1.1 Aspergillus niger , American Type Culture Collection No. 6275.21.1.2 Penicillium varians , American Type Culture Collection No. 10509.21.1.3 Trichoderma viride , American Type Culture Collection No. 28020 (see 24.5).21.2 Culture medium:21.2.1 Maintain stock culture of each of test tube slants of potato dextrose agar for A. niger and P . varians , and malt ex-tract agar for T. viride (see 24.6 and 24.8for media).21.2.2 Incubate new stock culture 7-10days at 25 ± 1°C (77 ± 2°F), then store at 2-10°C (36-50°F).21.3 Preparation of conidial suspen-sions:21.3.1 Conidial suspensions of fungal organisms are prepared by adding 10 mL of a sterile 0.5% saline solution contain-ing 0.05% of a non-fungicidal wetting agent (see 24.7) to a 7-10 day agar cul-ture.21.3.2 Scrape the surface of the culture gently with a platinum or nichrome wire to liberate the spores. Agitate the liquid slightly to disperse the spores without de-taching mycelial fragments, and gently decant the mold suspension into a flask containing a few glass beads.21.3.3 Shake the dispersion vigorously to break up any clumps of spores and then filter through a thin layer of sterile cotton or glass wool. Conidial suspen-sions may be stored at 6 ± 4°C (43 ± 7°F)for up to four weeks.21.3.4 Inoculum for test should be ad-justed using a hemocytometer or a Petroff-Hausser bacteria counter to con-tain five million conidia per mL on day of use by appropriate dilution of stock sus-pension with saline solution.21.4 Preparation of test specimens:21.4.1 To ensure luxuriant growth,both the test and control strips must be saturated with a sterilized glycerol nutri-ent solution of the following composi-tion: 97.6% distilled water, 2.0% glyc-erol, 0.1% K 2HPO 4, 0.1% NH 4NO 3,0.05% MgSO 4·7H 2O, 0.1% yeast extract and 0.05% of a nonionic wetting agent (see 24.7). Adjust the pH to 6.3 ± 0.1.Sufficient nutrient solution should be pre-pared to saturate all the specimens used in a single test.21.4.2 Soak each strip in nutrient for three minutes or until saturated. Squeeze excess liquid and allow fabric strips to air dry before proceeding with application of test fungi.21.5 Pre-mix equal volumes of well agitated conidial suspensions of A. niger ,T. viride and P . varians . Evenly distrib-ute 1.0 ± 0.1 mL of the above suspension onto both sides of each specimen either by spraying or by means of a pipette.21.6 Suspend fabric strips using plastic paper clips or nylon thread from the caps of individual jars containing 90 ± 3 mL of water each. Hook position must be ad-justed so that the bottom ends of attached strips are all at a uniform height above the water level. The caps are tightened,then backed off one-eighth turn to allow for some ventilation.21.7 Incubate at 28 ± 1°C (82 ± 2°F)for 14 days (for non-coated cellulosic textiles) or 28 days (for non-cellulosic or coated cellulosic textiles).22. Evaluation and Report22.1 A record of the percent of surface area covered with fungal growth for each strip is made at weekly intervals, or until heavy growth occurs on each sample rep-licate. Using a microscope (50X) where necessary, assess each specimen in accor-dance with the scheme given in 12.2.22.2 After seven days each control strip must show macroscopic growth. If this is not the case repeat the test since test conditions were not valid.22.3 Any adverse effect of incubation on the fabric; e.g., color changes, flexibil-ity, water repellency, should be qualita-tively reported.22.4 Strength loss determination can be carried out as per 8.1.22.5 The results of this test method have to be correlated to claims and direc-tions for use recommended for the mil-dew control product plus any other crite-ria agreed upon by the interested parties.23. Precision and Bias23.1 The precision and bias of this test method are being established. If the breaking strength loss is determined, then refer to the statement given in ASTM D5035, Standard Test Method for Break-ing Force and Elongation of Textile Fab-rics (Strip Method).24. Notes24.1 Publication available from U.S. De-partment of Health and Human Services, CDC/NIH-HHS Publication No. (CDC) 84-8395;web site: .24.2 Available from Publications Office,ACGIH, Kemper Woods Center, 1330 KemperCopyright © 2013 American Association of Textile Chemists and ColoristsCopyright The American Association of Textile Chemists and Colorists Provided by IHS under license with AATCC--`,,```,,,,````-`-`,,`,,`,`,,`---Meadow Dr., Cincinnati OH 45240; tel: +1. 513.742.2020; web site: . 24.3 A JDC Precision sample cutter may be purchased from Thwing-Albert Instrument Co., 10960 Dutton Rd., Philadelphia PA 19154; tel: +1.215.637.0100; fax: +1.215.632. 8370; web site: ; Cat. #99 Model JOC25.24.4 Types of soil which have been found satisfactory for this purpose include garden and naturally fertile topsoils, composts and non-sterile greenhouse potting soils. An equal blend of good topsoil, well rotted and shred-ded manure, and coarse sand should be used. These usually possess the proper physical characteristics, along with an organic content sufficient to ensure a high degree of microbial activity and the presence of cellulose destroy-ing organisms. The optimum moisture content of these is about 30% moisture above oven dry weight.24.5 Chaetomium globosum ATCC 6205, Aspergillus niger, ATCC 6275, Penicillium varians, A TCC 10509 and Trichoderma viride, A TCC 28020, can be purchased from the Amer-ican Type Culture Collection, P.O. Box 1549, Manassas V A 20108; tel: +1.703.365.2700; fax: +1.703.365.2701; web site: .24.6 Culture medium having composition prescribed in 11.2 (Mineral Salts) can be pur-chased from Baltimore Biological Laborato-ries, 250 Schilling Cir., Cockeysville MD 21030.24.7 Triton™ X-100 (Rohm & Haas Co.,Philadelphia PA 19104) has been found to be agood wetting agent. Suitable alternatives aredioctyl sodium sulfosuccinate or N-methyl-tauride derivatives.24.8 These culture media can be bought ei-ther from Baltimore Biological Laboratories(see 24.6) or Difco Laboratories, 920 HenrySt., Detroit MI 48201.24.9 ASTM D5035 can be used for yarn,thread, cordage or tape (see 12.1).24.10 If testing is being performed for Fed-eral Standards, use AATCC 30-2. Other or-ganisms can be used: Myrothecium verrucariaATCC 9095, QM 460; Trichoderma SP ATCC9645, QM 365; Memnoniella echinata ATCC11973, QM 1225; Aspergillus niger ATCC6275, QM 458; Aspergillus claratus ATCC18214, QM 862.Appendix APre-TreatmentsA1. LeachingA1.1 The leaching should conform inprinciple to the following procedure:Water from a faucet is passed through atubing into leaching vessels, care beingtaken that specimens having differentamounts of the same treatment are in sep-arate vessels. Flow is adjusted to ensure acomplete change of water not less thanthree times in 24 h. The delivery tubesare inserted down through the center ofwire mesh cylinders in the leaching ves-sels and held in the wire cylinders withrubber bands and leached for 24 h. ThepH and temperature of the water is re-corded and included in the report of thetest results.A2. VolatilizationA2.1 Standard specimens of the fabricto be tested are exposed continuously todry heat at 100-105°C (212-221°F) for24h in a well ventilated oven.A3. WeatheringA3.1 Portions of the material to betested are exposed on a series of weather-ing racks at 45° to the horizontal facingSouth, between April 1 and October 1, insuch a manner as to avoid sagging orflapping. It is recommended that suchracks be set up in at least four locationswithin the United States; e.g., Washing-ton DC; Miami FL; New Orleans LA;and suitable desert locations.--`,,```,,,,````-`-`,,`,,`,`,,`---。
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AATCC 30-2017防霉检测标准
AATCC 30-2017标准是一种广泛使用的测试方法，用于评估纺织材料对真菌生长的抵抗力。
这种测试方法可用于确定纺织品在潮湿和温暖环境中的耐霉变能力，以及其抵抗霉菌和腐烂的能力。
在AATCC 30-2017标准中，纺织材料的防霉性能是通过以下步骤进行评估的：
选取样品：从待测试的纺织材料中选取一定数量的样品，通常为10个或更多。
准备培养基：使用适当的培养基来支持真菌的生长。
常用的培养基包括沙氏培养基、孟加拉红培养基等。
接种真菌：将一定数量的真菌孢子接种到培养基上。
通常使用的真菌种类包括黑曲霉、绿色木霉等。
培养：将接种后的培养基放置在恒温、恒湿的环境中培养一定时间，以便真菌生长。
观察记录结果：定期观察培养基上的真菌生长情况，记录每个样品上真菌生长的情况。
根据真菌生长的程度，可以对纺织材料的防霉性能进行评估。
在实际操作中，可以根据需要对具体的培养条件、观察周期等进行适当调整。
例如，可以设置不同的湿度和温度条件，以模拟纺织材料在实际使用中的环境条件。
此外，可以根据需要选择不同的真菌种类进行测试，以评估纺织材料对
不同种类真菌的抵抗能力。
需要注意的是，AATCC 30-2017标准仅提供了一种评估纺织材料防霉性能的方法，其结果仅供参考。
在实际应用中，还需要考虑其他因素，如纺织材料的成分、结构、染料等对防霉性能的影响。
同时，如果待测试的纺织材料已经经过了防霉处理，则需要在测试中特别说明，以便对结果进行正确的解读。