化学发光法原理简介

化学发光法原理基本介绍Chemiluminescence immunoassay technology (CLIA) has been widely used in the fields of environmental chemistry, molecular biologics and medicine chemistry because of its wide linear range, fast and convenient operation, ease of automation, high accuracy and sensitivity features.

译：化学发光免疫分析技术（CLIA）因其检测范围很宽，操作方便快速，自动化程度高，极高的精密度和灵敏度等特征，已经广泛的应用到环境化学、药物化学和分子生物制剂等领域。Chemiluminescence immunoassay analyzer is used to detect the ultra-trace level of substances in human blood or other body fluids based on the principle of CLIA: at first according to theory of specific binding of antigen and antibody, the paramagnetic solid-phase carrier and sample (antigen) are bound with fluorescent-labeled antibodies (FLA) in the optimum condition, and then after inject H2O2 and NaOH solution, oxidation reaction makes the magnetic nanoparticles got in the first step jump to a unstable high-energy excited state and reduction reaction restores the excited state back to a ground state, the excessive energy during the reduction reaction is released in the form of photons; finally use photomultiplier tube to receive the photons and converted to

content information of the testing substance in the sample based on the build-in program.

译:化学发光免疫分析仪是在化学发光免疫分析技术（CLIA）的原理上用来检测人体血液和其他体液中的超微水平的物质，具体检测原理如下：1）根据抗原抗体特异性结合的原理，顺磁固相载体和样本（抗原）与荧光标记的抗体（FLA）以最合适的方式结合在一起；2）注入过氧化氢和氢氧化钠溶液后，氧化反应使第一部中的磁性纳米颗粒上升到高能激发态，而还原反应又将其从激发态恢复到基态，在这过程中多余的能量以光子的形式释放出来；3）用光电倍增管接受释放的光子，然后转化成样本中所要检测物质的含量信息。

The principle shows that the system needs a pipetting and measuring process. In this paper, the pipettor system makes use of contact sampling technique to improve the accuracy of pipetting. Considering a larger volume of fluorescence is dispensed into the sample solution in order to fully coated that the excessive fluorescence need to be removed otherwise the excessive photons will lead to a higher value of testing substance content. The paper removes the excess fluorescence-labeled primers in a washing method based on immunomagnetic separation technology and characteristic of magnetic solid-phase carrier at a magnetic field environment where can adsorb magnetic nanoparticles but release the excessive fluorescence free in the

solution.

译：从原理来看，整个检测系统需要一个吸收能量和测量的过程。本文中，移液系统通过抽样技术以提高移液的准确性。考虑到需要覆盖掉大量进入样本溶液的荧光因素，多余的荧光必须清除否则导致检测物质的数值偏高。我们在免疫磁珠分离技术的基础上通过液体洗涤的方式清除多余的荧光标记物质，同时液体环境中磁性固相载体在磁场中既可以结合磁珠又可以释放多余的荧光。