GM 1927-16b Aluminum Casting Impregnation Process Audit中英文

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艾森生物 实验室耗材和试剂 目录t

艾森生物 实验室耗材和试剂 目录t
G1626-100G T-200-Y
21127-030
P0018 31458
20W 500ml 500ml 100ml PL009 PL017 353002 3516
11644807001
2227S/100ul 3175S/100ul 7002s/100ul 7004s/100ul 9803s/15ml G7126-1KG
胎牛血清FBS MEM
FX 384 30ul 枪头
Horse serum马血清
DMSO
PMSF
25ml移液管
DMEM 细胞筛网 70um
细胞培养皿 65×15mm
384孔板
真空抽气泵
BSA牛血清蛋白
EGF Receptor (D38B1) XP® Rabbit mAb
western 一抗二抗去除液(弱碱性)
96孔细胞培养板 细胞网筛
25ml移液管 96孔板
无酚红1640 0.1-20ul排枪枪头 10-250ul排枪枪头 1ml盒装蓝色枪头 Trypsin EDTA,0.05%
DMSO 医用酒精棉球
医用酒精棉 麻面无粉手套 1ml灭菌盒装蓝枪头
DMEM L-15 384 HT assay plates
P3563-10PAK
PL017
SM0671/2x250ul
PL009 4685s/100ul 9271s/100ul
9101s/200ul
9102s/200ul 7002s/100ul 7004s/100ul 9803s/15ml
3777s/100ul
21127-022
11644807001 11966-025 6570 SD6031 华东 CU50
Gibco 华东 华东 Roche Gibco CORNING 上海生工 M号 中新 迪申 sigma AXYGEN Gibco

罗斯杰格高频技术公司产品7-16计ibration kit工业版说明书

罗斯杰格高频技术公司产品7-16计ibration kit工业版说明书

20/6.4D i e s e s D o k u m e n t i s t u r h e b e r r e c h t l i c h g e s c h üt z t ● T h i s d o c u m e n t i s p r o t e c t e d b y c o p y r i g h t ● R o s e n b e r g e r H o c h f r e q u e n z t e c h n i k G m b H & C o . K GPicture may differ from original productContentsDevicePart numberQuantityCalibration Option Open circuit plug 60S12L-000S3 1 FC / AC Open circuit jack 60K12L-000S3 1 FC / AC Short circuit plug 60S12S-000S3 1 FC / AC Short circuit jack 60K12S-000S3 1 FC / AC Calibration load plug 60S150-C10S3 1 FC / AC Calibration load jack 60K150-C10S3 1 FC / AC Calibration adaptor plug/plug 60S121-S20S3 1 FC / AC Calibration adaptor jack/jack 60K121-K20S3 1 FC / ACCombi wrench 60W013-000 1 - Torque wrench60W000-0031FCSee “Declaration of calibration options” for explanation.20/6.4D i e s e s D o k u m e n t i s t u r h e b e r r e c h t l i c h g e s c h üt z t ● T h i s d o c u m e n t i s p r o t e c t e d b y c o p y r i g h t ● R o s e n b e r g e r H o c h f r e q u e n z t e c h n i k G m b H & C o . K GIndustrial Version20/6.4D i e s e s D o k u m e n t i s t u r h e b e r r e c h t l i c h g e s c h üt z t ● T h i s d o c u m e n t i s p r o t e c t e d b y c o p y r i g h t ● R o s e n b e r g e r H o c h f r e q u e n z t e c h n i k G m b H & C o . K GDeclaration of Calibration OptionsFactory CalibrationStandard delivery for this kit includes a Factory Calibration. All devices marked with “FC” in the Content table above are reported in a Calibration Certificate with their individual calibration results, traceable to national / international standards. Model based definitions of the calibration standards are reported in Agilent/Keysight, Rohde & Schwarz and Anritsu compatible VNA format.Accredited CalibrationOptional this kit can be delivered with an Accredited Calibration (DAkkS) having the highest confidence in the traceability. All devices marked with “AC” in the Content table above are reported in a DAkkS Calibration Certificate with their individual calibration results in a complex format, traceable to national / international standards. Model based definitions of the calibration standards are reported in Agilent/Keysight, Rohde & Schwarz and Anritsu VNA format as well as in dense data sets needed for data based calibration kits. The uncertainties are a little bit smaller than in a Factory Calibration.All devices marked with “FC” only cannot be calibrated under accreditation. They are factory calibrated as described above.For further, more detailed information see application note AN001 on the Rosenberger homepage.Calibration IntervalRecommendation 12 monthsRecommended Accessories- Rosenberger Test Port Adaptor- Rosenberger VNA Test cable kit and Microwave Cable Assemblies - Rosenberger Gauge Kit 60GK0KS-010For further, more detailed information please visit our homepage .DraftDate Approved Date Rev. Engineering change numberName Date Martin Moder12.10.2015Chr. Janßen6.7.2022c0020-1927B. Wollitzer6.7.2022While the information has been carefully compiled to the best of our knowledge, nothing is intended as representation or warranty on our part and no statement herein shall be construed as recommendation to infringe existing patents. In the effort to improve our products, we reserve the right to make changes judged to be necessary. For the installation of the electrotechnical equipment, particular electrotechnical expertise is required.。

生工试剂

生工试剂

产品编号产品名称产品包装产品单价AE232-100G缓冲液100G441AE232-25G缓冲液25G117AE232-5KG ADA BUFFER5KGAE319-100ML PAGE电泳玻璃板硅化处理试剂100ML375AE319-12PK PAGE电泳玻璃板硅化处理试剂12PK426AE429-10MG抑肽酶10MG625AE434-100G琼脂糖100G1549AE434-1KG AgaroseⅣ1KGAE434-25G琼脂糖25G426AE434-5G琼脂糖5G98AE437-100MG两性霉素B100MG AE437-1G两性霉素B1GAE477-20ML氨苄青霉素钠20ML325AE531-0.5ML牛血清白蛋白标准溶液0.5ML229AE531-1.0ML牛血清白蛋白标准溶液 1.0ML380AE531-1.5ML牛血清白蛋白标准溶液·475AE566-1L ALKALINE TRANSFER SOLUTION AE566-250ML ALKALINE TRANSFER SOLUTION AE776-100G琼脂糖100G1142AE776-1KG Agarose3:01HRBAE776-25G琼脂糖25G343AE776-5G琼脂糖5G82AJ234-100G琼脂糖100G3015AJ234-25G琼脂糖25G829AJ234-2KG Agarose SFR2KGAJ234-5G琼脂糖5G198AJ365-100ML ABTS100ML389AJ468-100U琼脂糖酶-取消AJ581-5MG抗蛋白酶5MG472AJ582-50MG AEBSF50MG555AJ608-5MG放线菌素D5MG958AJ637-100G琼脂100G216AJ637-500G琼脂500G981AJ637-50KG AGAR50KG0ALS001041肌动蛋白-停产1MG468ALS001069乙醇脱氢酶-停止100MG601ALS001130醛缩酶100MG935ALS002763L-氨基酸氧化酶2MG695ALS002764L-氨基酸氧化酶5MG1456 ALS009043腺苷脱氨酶250U735AN0239A磷酸氢二铵500G26AN3795-100G对氨基笨磺酸100G24AN4306-100G对氨基酚100G78AN4306-2KG对氨基酚-CP2KG0AN4306-50G对氨基酚-CP50G0AN4307-100G邻氨基酚100G92AN4307-25G邻氨基酚-CP25G0AN4307-2KG邻氨基酚-CP2KG0AN5254-100G营养琼脂100G65AN5255-250G琼脂粉250G72AN5255-25KG琼脂粉25KG0AS0017-250G琼脂糖250G498AS0017-50G琼脂糖50G110AS1033-100G丙烯酰胺-5折优惠AT011高压灭菌指示带,2.5CM×55M卷98AT012高压灭菌指示带,1.8CM×50M卷78AT0173-500ML冰乙酸500ML49AT0216A氢氧化铵500ML24AT0216G氢氧化铵500ML35AT0785-500ML乙酸36%500ML32AT0850-500G氯化铵500G48AT0851-25KG硫酸铵-GR-5折优惠AT0851-500G硫酸铵-5折优惠AT0853-500G乙酸铵500G24AT0855-20KG草酸铵-GR20KG0AT0855-500G草酸铵500G34AT0856-500G碳酸铵500G26AT0857-500G过硫酸铵500G34AT0860-20KG磷酸二氢铵-GR20KG0AT0860-500G磷酸二氢铵500G34AT0861-20KG磷酸氢二铵-GR20KG0AT0861-500G磷酸氢二铵500G34AT0862-500G柠檬酸铵500G54AT0941-25G茜素绿25G98AT0941-500G茜素绿-BS500G0AT0949-25G茜素红25G19AT0957-25G苋菜红25G37AT0960-25G酸性品红25G26AT0971-25G茜素黄R25G17AT0972-25G茜素黄GG25G17AT0987-10G氨基黑10B10G28AT1014-25G L-抗坏血酸25G12AT1014-500G L-抗坏血酸-AR500G0AT1021-25G邻氨基苯甲酸25G32AT1021-500G邻氨基苯甲酸-AR500G0AT1022-25G对氨基苯甲酸25G32AT1024-25G1-氨基-2-萘酚-4-磺酸25G22AT1025-1G偶氮胂I1G62AT1026-1G偶氮胂III1G56AT1070-25G安替吡啉25G18AT1071-25G氨基比林25G19AT1074-25G4-氨基安替吡啉25G28AT1074-500G4-氨基安替吡啉-AR500G0AT1351-500G结晶氯化铝500G34AT1354-500G硝酸铝500G27AT1357-500G氢氧化铝500G26AT1358-500G中性氧化铝500G39AT1359-500G碱性氧化铝500G39AT1360-100G酸性氧化铝-AR100G0AT1360-500G酸性氧化铝500G39AT1569-20KG氯化铵-AR20KG0AT1569-500G氯化铵500G32AT1570-250G氟化铵-AR250G0AT1570-500G氟化铵500G28AT1574-500G乙酸铵500G26AT1575-10KG草酸铵-AR10KG0AT1575-500G草酸铵500G32AT1578-500G磷酸铵500G26AT1583-100G偏钒酸铵100G38AT1587-10KG碳酸氢铵-AR10KG0AT1587-500G碳酸氢铵500G22AT1595-500G磷酸二氢铵500G26AT1597-500G柠檬酸铁铵500G44AT1600-10KG柠檬酸铵-AR10KG0AT1601-500G柠檬酸铵500G38AT1603-500G500G0AT1620-500ML苯胺500ML29AT1634-100G盐酸苯胺100G54AT1706-500ML乙酸36%500ML19AT1876-100G2-氨基噻唑100G27AT1876-2KG2-氨基噻唑-AR2KG0AT1921-500ML40%乙醛500ML34AT2076-5G丙氨酸5G20AX174-100G琼脂糖100G1816AX174-1KG AgaroseⅢ1KGAX174-250G AgaroseⅢ250GAX174-25G琼脂糖25G499AX174-5G琼脂糖5G110B0010-0.1OD M13/pUC Sequencing B0011-0.1OD M13/pUC Sequencing B0011-1.0OD M13/pUC Sequencing B0012-0.1OD M13/pUC Sequencing B0012-1.0OD M13/pUC Sequencing B0013-0.1OD M13/pUC ReverseB0013-1.0OD M13/pUC ReverseB0081-1000U钻石Taq酶1000UB0081-200U钻石Taq酶200UB0089-1000U Taq酶-B0089-200U Taq酶-B0089-5000U Taq酶-B0090-100U Taq Plus100UB0090-200U Taq Plus200UB0090-500U Taq Plus500UB0093-100U Pfu DNA Polymerase B0093-200U Pfu DNA Polymerase B0093-500U Pfu DNA Polymerase B0095-100U Heatstart Taq DNAB0095-200U Heatstart Taq DNAB0095-500U Heatstart Taq DNAB01BUFFER5*1ML152B0147Cohesive-end adaptor0.5B0148Duplex0.5ODB0172-100G双丙烯酰胺100G349B0172-10KG BIS-ACRYLAMIDE10KG0B0172-25G双丙烯酰胺25G96B19Diluent Buffer SetB22Buffer for Eco52I+B2292-6福尔马林4L284B23Buffer for SduI+B24Buffer for ScaI+B25Buffer for Eam1105I+ B26Buffer for Ecl136II+ B27Buffer for Bpu10I+B28Buffer for TaqI+B29Buffer for KpnI+B30Buffer5*1ML152B57Buffer for BamHI+BB0025-100G双丙烯酰胺-5折优惠BB0025-25G双丙烯酰胺-5折优惠BB0025-25KG BIS-ACRYLAMIDE-5折优惠BB0044-1KG硼酸-5折优惠BB0072-100MG5-溴-4-氯-3-吲哚基磷酸酯钠100MG144BB0072-500MG5-溴-4-氯-3-吲哚基磷酸酯钠500MG664BB0078-500MG D-生物素500MG204BB0078-5G D-生物素5G1298BB0079-100G BIS-TRIS-7折优惠BB0079-500G BIS-TRIS-7折优惠BB0083-100G X-Gal-5折优惠BB0083-1G X-Gal-5折优惠BB0083-500MG X-Gal-5折优惠BB0085-100MG X-GLUC100MG1078BB0085-5G X-GLUC5G0BB0114-100G牛肉浸膏100G280BB0114-15KG牛肉浸膏15KG0BB0114-500G牛肉浸膏500G1190BB0260-1G BCA1G304BB0260-25G BCA25G0BB0260-5G BCA-没有折扣BB0266-100G N,N-二-羟乙基甘氨酸-7折优惠BB0266-25G N,N-二-羟乙基甘氨酸-7折优惠BB0443-25G溴百里酚蓝25G698BB0443-5G溴百里酚蓝5G171BB0637-1KG聚环氧依稀月桂酰醚1KG287BB0637-250G聚环氧依稀月桂酰醚250G88BB1111-1G碱性品红1G62BB1111-25G碱性品红25G1048BB1111-5G碱性品红5G0BB1147-100G苯甲酸100G52BB1147-1KG苯甲酸1KG448BB1160-100ML BCIP×NBT100ML398BB1160-1L BCIP×NBT1L0BB1160-25ML BCIP×NBT25ML109BB1168-100MG X-GLUC-NA100MG962BB1168-20MG X-GLUC-NA20MG245BB1170-100ML BCIP×INT100ML554BB1170-25ML BCIP×INT25ML166BB1171-100MG5-溴-4-氯-3-吲哚基磷酸酯钠100MG158BB1171-1G5-溴-4-氯-3-吲哚基磷酸酯钠1G1173BB1171-500MG5-溴-4-氯-3-吲哚基磷酸酯钠500MG655BB1171-5G BCIP-Na25G0BB1182-1G BLUO-GAL1G1822BB1182-500MG BLUO-GAL500MG998BB2230-100G溴酚蓝100G996BB2230-25G溴酚蓝25G278BB2230-5G溴酚蓝5G76BB5BUFFER B+5MLBD02-0.05MG T7DNA Phage BD02-0.25MG T7DNA Phage BD03-0.05MG pBR322DNA0.05MG BD03-0.25MG pBR322DNA0.25MG BD10-0.05MG LambdaDNA(dam,dcm)0.05MG90BD10-0.25MG LambdaDNA(dam,dcm)0.25MG360BD11-0.05MG Lambd DNA0.05MG BD11-0.25MG Lambda DNA0.25MG BE116-100ML BCIP×NBT100ML357BE116-25ML BCIP×NBT25ML98BE410-25G碱性品红-7折优惠BE410-5G碱性品红-7折优惠BE530-100ML BRADFORD REAGENT100ML BE530-1L BRADFORD REAGENT1LBE535-1KIT BRADFORD METHOD1KITBE671-1L BIO-BLOCK IN PBSBE692-100ML BCIP×INT100ML479BE692-25ML BCIP×INT25ML132BE952-500KU杆菌肽500KU492BG5BUFFER G+5MLBJ472-1G BIG CHAP1GBJ472-250MG BIG CHAP250MGBJ472-5G BOG CHAP5GBJ579-10MG贝他定10MG1379BK186-100G盐酸甜菜碱100G78BK186-1KG Betaine hydrochloride1KGBK186-500G盐酸甜菜碱500G324100MLBK217-100ML BLUE-BANDIT蛋白质电冰染色液BK217-1L BLUE-BANDIT PROTEIN STAIN500MLBK217-500ML BLUE-BANDIT蛋白质电冰染色液BM101Diamond TM250bpBM102Diamond TM250bpBM201-100μL Protein Marker,Low RangeBM201-30μL Protein Marker,Low RangeBM225-100G BES100G720BM225-25G BES25G198BM225-500G BES500G0BM523Protein Marker,Mid Range,ready BM524Protein Marker,Mid RangeBM525Pre-stained Protein Marker,Mid BN3002A硼砂500G40BN5216-1G6-苄氨基嘌呤1G40BN5216-5G6-苄氨基嘌呤5G188BN5244-100G牛肉浸膏100G49BN5244-20KG BEEF EXTRACT20KGBN5244-500G牛肉浸膏500G225BN5245-500G酵母浸膏500G64BO5BUFFER O+5MLBP10移液器,0.5-10μL支880BP100移液器,10-100μL支880BP1000移液器,100-1000μL支880BP2移液器,0.1-2.5μL支880BP20移液器,2-20μL支880BP200移液器,20-200μL支880BP50移液器,5-50μL支880BP5000移液器,1000-5000μL支880BS243双链DNA补平和连接试剂盒10次460BS244双链DNA补平和连接试剂盒20次820BS249MMLV First StrandBS250MMLV First StrandBS251AMV First StrandBS252AMV First StrandBS293Ready-to-Use PCR KitBS294Ready-to-Use PCR KitBS295HiFi Ready-to-Use PCRBS296HiFi Ready-to-Use PCRBS297Genepha Long PCRBS298Genepha Long PCRBS353UNIQ-10柱式DNA胶回收试剂盒50次300BS354UNIQ-10柱式DNA胶回收试剂盒100次500BS363UNIQ-10柱式PCR产物纯化试剂盒50次290BS364UNIQ-10柱式PCR产物纯化试剂盒100次480BS382PROTEASE INHIBITOR COCKTAIL BS387PROTEASE INHIBITOR COCKTAIL BS409Trizol25ML190BS410Trizol100ML720BS413UNIQ-10柱式质粒小量抽提试剂盒50次310BS414UNIQ-10柱式质粒小量抽提试剂盒100次53050次410BS423UNIQ-10柱式细菌基因组DNA抽提试BS424UNIQ-10柱式细菌基因组DNA抽提试100次760BS433pUCm-T Vector1μGBS434pUCm-T Vector5μGBS435PCR产物克隆试剂盒20次320BS436PCR产物克隆试剂盒100次129010次500BS463UNIQ-200柱式质粒中量抽提试剂盒BS464UNIQ-200柱式质粒中量抽提试剂盒20次80010次1000BS465UNIQ-500柱式质粒小量抽提试剂盒20次1800BS466UNIQ-500柱式质粒小量抽提试剂盒BS473经典基因组DNA抽提试剂盒50次350BS474经典基因组DNA抽提试剂盒100次42050次350BS483EZ-10柱式血液基因组DNA抽提试剂100次680BS484EZ-10柱式血液基因组DNA抽提试剂BS503Quik LB AgarBS511快速连接试剂盒20次140BS512快速连接试剂盒100次560BS513平端DNA片段添A试剂盒40次330BS514平端DNA片段添A试剂盒100次760BS523一步法制备感受态细胞溶液25ML200BS524一步法制备感受态细胞溶液50ML350BS525高效感受态细胞制备试剂盒50ML160BS526高效感受态细胞制备试剂盒100ML290BS573经典总RNA抽提试剂盒20次280BS574经典总RNA抽提试剂盒100次1080BS583UNIQ-10RNA抽提BS584UNlQ-10柱式总RNA抽提试剂盒100次1120BS589MAMMALIAN PROTEIN EXTRACTION BS596BACTERIAL PROTEIN EXTRACTION BS633UNlQ-10柱式mRNA抽提纯化试剂盒10次800BS634UNlQ-10柱式mRNA抽提纯化试剂盒20次1270BS653MMLV Single StepBS654MMLV Single StepBS663AMV Single StepBS664AMV Single StepBS686INCLUSION BODY PROTEINBS689-A IMMUNOPRECIPITATION KIT50次BS694Components sold separately BS721-20ML两性霉素B溶液-5折优惠BS722-20ML氨苄青霉素钠溶液-5折优惠BS723-5ML G418SULFATEBS724-20ML硫酸庆大霉素,R-射线灭菌-5折优惠BS725-20ML HYGROMYCIN B-BS725-2ML R-射线灭菌-5折优惠BS726-210ML硫酸卡那霉素-5折优惠BS727-20ML硫酸新霉素溶液(射线灭菌)-5折优惠BS728-20ML制霉菌素溶液(R-射线灭菌)-5折优惠BS729-2*10ML青霉素G溶液-5折优惠BS730-2*10ML硫酸链霉素溶液(R-射线灭菌)-5折优惠BS731-2*10ML盐酸四环素溶液-5折优惠BS732-2*10ML青霉素/链霉素-5折优惠BS733-2*10ML青霉素/链霉素/两性霉素B-5折优惠BS734-2*10ML青霉素/链霉素/新霉素-5折优惠BS801Quik LB AmpBS802Quik LB AgarBS803Quik LB AgarBS804Quik LB KanBS805Quik LB AgarBS806Quik LB AgarBS858HYLB-Capsule20capsulesBS859-100LB Broth-Capsule100BS859-20LB Broth-Capsule20BS860-100LB Agar-Capsule100BS860-20LB Agar-Capsule20BS861-20LB#NAME?Agar-Capsule BS862-20LB Lennox-Capsule20BS863-20LB Lennox Agar-Capsule BS864-20SOB Broth-Capsule20BS865-20SOB Agar-Capsule20BS866-20SOC Broth-Capsule20BS867-20NZCYM Broth-Capsule20BS868-20NZCYM Agar-capsule20BS869-20NZYM-Capsule20capsulesBS870-20NZYM Agar-Capsule20BS871-20NZM Broth-capsule20BS872-20NZM Agar-Capsule20BS873-20YT-Capsule20capsulesBS874-20YT Agar-Capsule20BS875-20YT-Top Agar-Capsule20BS876-202×YT-Capsule20capsulesBS877-202×YT Agar-Capsule20BS878-202×YT Top Agar-Capsule BS879-20YPD Broth-Capsule20BS880-20YPD Agar-Capsule20BS881-20M9Broth-Capsule20BS882-20M9CA Broth-Capsule20BS883-20Super Broth-Capsule20BS884-20Super Broth Agar-Capsule BS885-20Super Broth TopBS886-20Lambda Broth-Capsule20BS887-20Tryptone Broth-Capsule20BS888-20YM#NAME?20BS889-20TYGPN-Capsule20capsulesBS890-20M63Broth-Capsule20BS891-20YM Agar-Capsule20BS892-20Terrific broth-Capsule20BT0897-100KG硼酸-GR100KG0BT0897-500G硼酸500G34BT0901-250G苯甲酸250G29BT0922-1KG溴酚蓝-IND1KG0BT0922-25G溴酚蓝25G28BT0943-5G溴甲酚绿5G24BT0944-10G溴甲酚绿钠盐10G38BT0944-250G溴甲酚绿钠盐-AR250G0BT0956-5G溴酚红5G26BT0959-25G碱性品红25G19BT0962-25G曙红Y,醇溶25G39BT0963-25G曙红Y,水溶25G39BT0968-10G溴甲酚紫10G42BT0981-25G备士麦棕Y25G22BT0990-25G巴比妥钠25G70BT1018-1G吲哚-3-乙酸1G16BT1019-1G吲哚-3-丁酸1G16BT1127-25G煌橙25G32BT1128-25G苄橙25G32BT1135-1KG巴比妥酸-AR1KG0BT1135-25G巴比妥酸25G21BT1497-500G氯化钡500G27BT1500-500G硫酸钡500G26BT1501-500G硝酸钡500G24BT1502-500G乙酸钡500G43BT1504-500G氢氧化钡500G26BT1679-500G硼酸-5折优惠BT1695-250G苯甲酸250G19BT1735-250G反丁烯二酸-AR250G0BT1735-500G反丁烯二酸500G29BT1737-100G焦性没食子酸100G48BT1743-250G苯甲醇250G37BT1743-5KG苯甲醇-AR5KG0BT1972-500G双酚A500G30BT1972-5KG双酚A-AR5KG0BT2062-25G牛黄酸25G22C0101-1KG柠檬酸钠二水1KGC0101-500G柠檬酸钠二水500G50KGC0101-50KG SODIUM CITRATE,TRISODC0102-10G二甲基胂酸10G131C0102-250G二甲基胂酸250G2683C0102-50G二甲基胂酸50G590C0164-1G胰凝乳蛋白酶1G838C0164-250MG胰凝乳蛋白酶250MG252C0164-5G胰凝乳蛋白酶5G3810C0179-100G碳酸钙无水100GC0179-1KG碳酸钙无水1KG20KGC0179-20KG CALCIUM 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CHES25G180C0398-100G4-氯-1-萘酚100G1618C0398-1KG4-CHLORO-1-NAPHTHOL1KG0C0398-25G4-氯-1-萘酚25G445C0398-5G4-氯-1-萘酚5G107C0412-1G胞嘧啶1G73C0412-25G胞嘧啶25G1327C0412-5G胞嘧啶5G305C0415-100G氯化铯100G448C0415-20KG CESIUM CHLORIDE20KGC0415-25G氯化铯25G121C0415-500G氯化铯500G1996C0433-100G胆固醇100G238C0433-1KG CHOLESTEROL1KG0C0433-25G胆固醇25G72C0433-500G胆固醇500G1021C0436-1G甲酚红1G32C0436-25G甲酚红25G482C0436-5G甲酚红5G106C0465-1G CHAPS-7折优惠C0465-25G CHAPS-7折优惠C0465-500G CHAPS-7折优惠C0465-5G CHAPS-7折优惠C0472-100G考马士亮兰R-250-C0472-1KG COOMASSIE BRILLIANT BLUEC0472-25G考马士亮兰R-250-C0472-5G考马士亮兰R-250-C0473-100G硫酸铯100G1034C0473-25G硫酸铯25G286C0473-5G硫酸铯5G75C0500-1G甲酚红钠盐1G30C0500-25G甲酚红钠盐25G477C0500-5G甲酚红钠盐5G105C0528-100G结晶紫100G303C0528-25G结晶紫25G80C0528-500G结晶紫500G1376C0529-1KG柠檬酸1KG262C0529-20KG CITRIC ACID20KGC0529-250G柠檬酸250G74C0556-1KG氯化钙二水-没有折扣1KGC0556-250G氯化钙二水250GC0556-500G氯化钙二水500G50KGC0556-50KG CALCIUM CHLORIDE,DIHYDC0611-100MG5-三磷酸胞苷三钠盐100MG675C0611-1G CYTIDINE-5-TRIPHOSPHATE,TRIS1G0C0611-50MG5-三磷酸胞苷三钠盐50MG372C0615-100G考马士亮兰G-250-C0615-2.5KG COOMASSIE BRILLIANT BLUEC0615-25G考马士亮兰G-250-C0615-5G考马士亮兰G-250-C0707-100G1-氯-2,4-二硝基苯100G258C0707-25G1-氯-2,4-二硝基苯25G77C0707-500G1-氯-2,4-二硝基苯500G1075C0758-100G氯化十六烷基吡啶100G145C0758-30KG CETYLDIMETHYLETHYL AMMONIUM BROMIDE C0758-500G氯化十六烷基吡啶500G658C0833-100G十六烷基-三甲基-溴化铵100G105C0833-1KG十六烷基-三甲基-溴化铵1KG819C0833-25KG CTAB25KG0C0833-500G十六烷基-三甲基-溴化铵500G447C0887-100G氯化铯100G359C0887-25G氯化铯25G98C0887-500G氯化铯500G1632C0887-5KG氯化铯5KG0C94271-100MG放线菌酮(环已酰亚胺)100MG299C94271-1G放线菌酮(环已酰亚胺)1G1182 CA33112DIGIT-12项功能1个36CA33210DIGIT,56项功能1个25CB0035-1KG柠檬酸钠二水-CB0035-500G柠檬酸钠二水-CB0037-100G考马士亮兰R-250-CB0037-25G考马士亮兰R-250-CB0037-5G考马士亮兰R-250-CB0038-100G考马士亮兰G-250-CB0038-25G考马士亮兰G-250-CB0038-5G考马士亮兰G-250-CB0051-100G硫酸铯100G1156 CB0051-25G硫酸铯25G351CB0051-5G硫酸铯5G95CB0052-100G碳酸钙无水100G CB0052-1KG碳酸钙无水1KGCB0052-500G碳酸钙无水500G CB0054-100G氯化铯100G413CB0054-25G氯化铯25G114CB0054-500G氯化铯500G1960 CB0055-1KG柠檬酸-7折优惠CB0055-250G柠檬酸-7折优惠CB0088-100G L-胱氨酸100G68CB0088-1KG L-CYSTINE1KG0CB0088-500G L-胱氨酸500G252CB0097-250G3-(环已氨基)-1-丙磺酸-7折优惠CB0097-50G3-(环已氨基)-1-丙磺酸-7折优惠CB0105-100G十六烷基-二甲基-乙基-溴化铵100G198CB0105-25G十六烷基-二甲基-乙基-溴化铵25G65CB0105-500G十六烷基-二架基-乙基-溴化铵500G916CB0108-100G十六烷基-三甲基-溴化铵-7折优惠CB0108-1KG十六烷基-三甲基-溴化铵-7折优惠CB0108-500G十六烷基-三甲基-溴化铵-7折优惠CB0115-250G CHES-7折优惠CB0115-25G CHES-7折优惠CB0118-100G氯霉素-5折优惠CB0118-25G氯霉素-5折优惠CB0118-25KG氯霉素-5折优惠CB0124-1G6-氯嘌呤-取消CB0124-250MG6-氯嘌呤-取消CB0124-25G6-CHLOROPURINE5G0CB0132-100G L-半胱氨酸100G134CB0132-500G L-半胱氨酸500G530CB0132-50G L-半胱氨酸50G72CB0132-5KG L-CYSTEINE5KG0CB0133-100G L-半胱氨酸盐酸盐100G256CB0133-25G L-半胱氨酸盐酸盐25G77CB0152-1G CIBACRON BRILLIANT REDCB0152-25G CIBACRON BRILLIANT REDCB0152-5G CIBACRON BRILLIANT REDCB0153-1G CIBACRON BRILLIANT YELLOW CB0153-25G CIBACRON BRILLIANT YELLOW CB0153-5G CIBACRON BRILLIANT YELLOW CB0155-1G汽巴蓝F3GA1G30CB0155-5G汽巴蓝F3GA5G106CB0201-1K KU胰凝乳蛋白酶-CB0201-250KU胰凝乳蛋白酶-取消CB0321CTP25UMOL135CB0321-1ML CTP(100mM)1ML0CB0351-100ML氯仿:异戊醇(24:1)100ML118CB0351-200ML氯仿:异戊醇(24:1)200ML192CB0351-950ML CHLOROFORM:ISOAMYL ALCOHOL(24:1)950ML CB3060-100G酪蛋氨基酸(酪蛋白水解物)-没100G60CB3060-4*500G酪蛋氨基酸(酪蛋白水解物))-4*500G950CB3060-500G酪蛋氨基酸(酪蛋白水解物))-500G250CB3060-70KG酪蛋氨基酸(酪蛋白水解物)70KG0CB4211-1G天青石蓝1G75CB4211-5G天青石蓝5G312CB5277-1G头孢菌素C锌盐1G71 CB5277-25G头孢菌素C锌盐25G1463 CB5277-5G头孢菌素C锌盐5G322 CE203-5ML即用型细菌细胞裂解液5ML745 CE302-1G CHAPSO1G370 CE302-50G CHAPSO50G0CE302-5G CHAPSO5G1609 CE506-100ML1M氯化钙溶液100ML CE506-500ML1M氯化钙溶液500ML CLS001260碳酸酐酶50MG321 CLS001263碳酸酐酶250MG1069 CLS001333胰凝乳蛋白酶1G227 CLS001334胰凝乳蛋白酶10G1604 CLS001430胰凝乳蛋白酶25MG241 CLS001432胰凝乳蛋白酶100MG755 CLS001448胰凝乳蛋白酶250MG334 CLS001641CLOSTRIPAIN1MG0 CLS001652胶原(蛋白)5G468 CLS001654胶原(蛋白)1G132 CLS001656胶原(蛋白)10G735 CLS001722羧肽酶B1KU401 CLS001724羧肽酶B3KU869 CLS001847过氧化氢酶2G454 CLS001849过氧化氢酶10G1871 CLS001872过氧化氢酶10ML200 CLS001873过氧化氢酶100ML935 CLS001896过氧化氢酶10ML214 CLS001898过氧化氢酶100ML1403 CLS002598纤维素酶250MG307 CLS002601纤维素酶1G762 CLS002610纤维素酶1G401 CLS002611纤维素酶10G3140 CLS003708胰蛋白酶100MG174 CLS004103胆淄醇酯酶100U401 CLS004104胆淄醇酯酶500U1510 CLS004174胶原酶2型100MG321 CLS004177胶原酶2型5G0 CLS004180胶原酶3型100MG321 CLS004182胶原酶3型1G1871 CLS004183COLLAGENASE,TYPE35G0 CLS004186胶原酶4型100MG321 CLS004188胶原酶4型1G1871 CLS004189COLLAGENASE,TYPE45G0 CLS004194胶原酶1型100MG321 CLS004196胶原酶1型1G1871 CLS004197COLLAGENASE,TYPE15G0 CLS004202胶原酶2型50MG374CLS004204胶原酶2型250MG1443 CLS004206胶原酶3型50MG374 CLS004208胶原酶3型250MG1443 CLS004210胶原酶4型50MG374 CLS004212胶原酶4型250MG1443 CLS004214胶原酶1型50MG374 CLS005273胶原酶10KU1804 CLS005301羧肽酶B10MG962 CLS005304羧肽酶B50MG4276 CLS005305羧肽酶B5MG641 CLS005623CHYMOTRYPSINOGEN A,PURIFIED5G CLS005630胰凝乳蛋白酶原1G481 CLS005632胰凝乳蛋白酶原5G1737 CLS009068羧肽酶Y5MG3341 CLS009070羧肽酶Y1MG802 CLT001弹簧水夹,不锈钢,40MM4个15 CLT002弹簧水夹,不锈钢,60MM4个17 CLT003弹簧水夹,弹力钢,80MM4个20 CLV001铜,260MM,爪式万能夹1个23 CN0721-500G磷酸氢钙500G74 CN0721C磷酸氢钙500G74 CN1138-1KG卡那胶1KG350 CN1138-20KG卡那胶20KG0 CN1138-500G卡那胶500G180 CN3924A-250G水合氯醛250G42 CN5204-500G干酪素500G98 CT0123-500ML氯仿(三氯甲烷)500ML60 CT0124-4L氯仿(三氯甲烷)4L398 CT0124-500ML氯仿(三氯甲烷)-HPLC500ML0 CT0914-10G邻甲酚酞10G20 CT0928-100G克罗拉卓蓝100G35 CT0933-5G考马士亮兰G-2505G CT0934-10G考马士亮兰R-250-BS10G CT0934-500G考马士亮兰R-250-BS500G CT0934-5G考马士亮兰R-2505G CT0953-25G甲酚红25G26 CT0953-500G甲酚红-AR500G0 CT0954-250G刚果红-IND250G0 CT0954-25G刚果红25G28 CT0966-25G结晶紫25G16 CT0966-500G结晶紫-AR500G0 CT0969-10G间甲酚紫10G34 CT0969-200G间甲酚紫-AR200G0 CT0970-5G邻苯耳酚紫5G26 CT0975-100G香豆素100G48 CT0976-5G姜黄素5G44 CT0978-5G钙黄绿素5G20CT0985-25G铬黑T25G22 CT0986-250G依来铬蓝黑R-IND250G0 CT0986-25G依来铬蓝黑R25G19 CT1004-250G变色酸-AR250G0 CT1004-25G变色酸25G34 CT1027-10G偶氮氯膦I-AR10G CT1027-1G偶氮氯膦I1G CT1028-1G偶氮氯膦III1G CT1059-25G邻甲酚酞络合指示剂25G78 CT1105-25G辛可宁25G238 CT1110-10G铬天青S10G29 CT1112-25G D-泛酸钙25G26 CT1114-100G氯化胆碱100G34 CT1136-250G黄七精Y-IND250G0 CT1136-25G黄七精Y25G118 CT1143-10G盐基淡黄10G32 CT1314-250G碳酸钙250G25 CT1315-500G硝酸钙500G26 CT1317-500G乙酸钙500G29 CT1318-500G草酸钙500G34 CT1321-500G氧化钙500G0 CT1322-500G氟化钙500G29 CT1325-250G氢氧化钙-AR250G0 CT1325-500G氢氧化钙500G22 CT1326-25KG柠檬酸钙-AR25KG0 CT1326-500G柠檬酸钙500G32 CT1330-500G无水氯化钙500G26 CT1331-500G氯化钙二水500G CT1425-500G硫酸铜五水500G CT1426-10KG硝酸铜-AR10KG0 CT1427-500G乙酸铜500G64 CT1429-10KG氯化铜-AR10KG0 CT1429-500G氯化铜500G48 CT1434-10KG氢氧化铜-AR10KG0 CT1434-250G氢氧化铜-AR250G0 CT1434-500G氢氧化铜500G57 CT1439-100G酒石酸铜100G52 CT1444-500G无水硫酸铜500G39 CT1459-100G硫酸钴100G0 CT1460-100G硝酸钴100G34 CT1460-2KG硝酸钴-AR2KG0 CT1461-100G乙酸钴100G38 CT1463-100G草酸钴100G59 CT1464-100G氯化钴100G29 CT1464-5KG氯化钴-AR5KG0 CT1482-500G硝酸铬500G52 CT1484-500G氧化铬500G64CT1485-500G氯化铬500G0CT1490-250G氧化镉250G0CT1491-250G氯化镉250G49CT1492-100G乙酸镉100G47CT1493-100G硫酸镉100G0CT1495-250G硝酸镉250G68CT1542-1KG活性碳粉状1KGCT1542-500G活性碳粉状-AR500GCT1543-1KG活性碳柱状1KGCT1544-1KG活性碳颗粒状1KGCT1544-500G活性碳颗粒状-AR500G(用于1KG) CT1611-100G十六烷基-三甲基-溴化铵-5折优惠CT1614-25G十六烷基-三甲基-氯化铵25G46CT1702-500G柠檬酸-5折优惠CT1705-500G氯乙酸500G28CT1707-500ML正辛酸500ML44CT1859-500ML蓖麻油500ML32CT1860-25ML香柏油25ML14CT2004-500ML氯仿(三氯甲烷)500ML28CT2041-100G肌酐-BR100G0CT2041-5G肌酐5G98CT2042-25G肌醇25G14CT2042-500G肌醇-BR500G0CT2042-5KG肌醇-BR5KG0CT2056-25G肌酸25G19CT2057-25G胆酸25G72CT2121-500G环糊精500G39CT2149-100G乳酸钙100G22CT2149-5KG乳酸钙-BR5KG0CT2154-25G泛酸钙25G24CT2154-500G泛酸钙-BR500G0D0044-1DATP-低于5折优惠D0046-1DCTP-低于5折优惠D0048-1DGTP-低于5折优惠D0050-1DTTP-低于5折优惠D0055DNTP-低于5折优惠D0056dNTP-低于5折优惠D0081-1000U Tsg DNA PolymeraseD0081-200U Tsg DNA PolymeraseD0081-5000U Tsg DNA PolymeraseD0198-10G硫酸葡聚糖10G146D0198-10KG DEXTRAN SULFATE10KGD0198-250G硫酸葡聚糖250G2438D0231-100ML二甲亚砜100ML49D0231-20L DMSO20L0D0231-500ML二甲亚砜500ML188D0238-1KG二乙醇胺1KG229D0238-2.5KG DIETHANOLAMINE 2.5KG0D0238-250G二乙醇胺250G65D0238-500G DIETHANOLAMINE(用于1KG)500G0D0293-250ML二乙胺250ML93D0293-950ML二乙胺950ML322 D0425-1G二硫赤藓糖醇1G81D0425-25G DTE25G0D0425-5G二硫赤藓糖醇5G349 D0430-100G DAB,TETRAHYDROCHLORIDE100G0D0430-1G3,3二氨基联苯胺四盐酸二水D0430-25G3,3二氨基联苯胺四盐酸二水D0430-5G3,3二氨基联笨胺四盐酸二水D0435-10G DANSYL CHLORIDE10G D0435-1G丹磺酰氯1G207 D0435-5G丹磺酰氯5G928 D0445-10G DEAE葡聚糖10G109 D0445-250G DEAE DEXTRAN250G D0445-50G DEAE葡聚糖50G480 D0456-100G2’-DEOXYADENOSINE,MONOHYDRA100G0D0456-1G2’-脱氧腺苷一水1GD0456-5G2’-脱氧腺苷一水5GD0464-20L N’,N-DIMETHYLFORMAMIDE20L0D0464-250ML N’,N-二甲基甲酰胺250ML73D0464-500ML N’,N-二甲基甲酰胺500ML113 D0467-100G2’-DEOXYCYTIDINE,HYDROCHLOR100G0D0467-1G2’-脱氧胞苷盐酸盐1G272 D0467-5G2’-脱氧胞苷盐酸盐5G1235 D0475-100G2-DEOXYGUANOSINE,MONOHYDRATE100G0D0475-100MG2’-脱氧鸟苷一水100MG D0475-1G2’-脱氧鸟苷一水1GD0475-500MG2’-脱氧鸟苷一水500MG D0481-1G2’-脱氧胸苷1G81D0481-25G2’-脱氧胸苷25G1666 D0481-5G2’-脱氧胸苷5G367 D0491-1G2’-脱氧尿苷1G583 D0491-250MG2’-脱氧尿苷250MG169 D0491-5G2’-脱氧尿苷5G2650 D0503-100MG5’-三磷酸-2’-脱氧腺苷二钠盐100MG1832 D0503-50MG5’-三磷酸-2’-脱氧腺苷二钠盐50MG941 D0518-100MG5’-三磷酸-2’-脱氧胞苷三钠盐100MG1390 D0518-50MG5’-三磷酸-2’-脱氧胞苷三钠盐50MG763 D0526-250MG5’-三磷酸-2’-脱氧鸟苷三钠盐250MG4000 D0526-50MG5’-三磷酸-2’-脱氧鸟苷三钠盐50MG881 D0532-100MG5’-三磷酸-2’-脱氧胸苷三钠盐100MG1994 D0532-50MG5’-三磷酸-2’-脱氧胸苷三钠盐50MG1200 D0613-10G脱氧胆酸钠盐10G67D0613-250G脱氧胆酸钠盐250G1391D0613-500G DEOXYCHOLIC ACID SODIUM D0613-50G脱氧胆酸钠盐50G306D0624-100ML十二烷基-三甲基-氯化铵100ML256D0624-25ML十二烷基-三甲基-氯化铵25ML85D0624-500ML十二烷基-三甲基-氯化铵500ML1096 D0644-100G DNA100G0D0644-1G DNA1G68D0644-25G DNA25G1290 D0644-500G DNA500G0D0644-5G DNA5G284D0649-100KU脱氧核糖核酸酶I100KU D0649-50KU脱氧核糖核酸酶I50KU D0657-1G2’-脱氧-D-核糖1G126D0657-50G2-DEOXY-D-RIBOSE50G0D0657-5G2’-脱氧-D-核糖5G572D0665-100G葡聚糖100G858D0665-1KG DEXTRAN1KG0D0665-25G葡聚糖25G236D0665-5G葡聚糖5G59DT0119-500ML二氯甲烷500ML65DT0120-4L二氯甲烷4L430 DT0120-500ML二氯甲烷-HPLC500ML0DT0151-500ML N,N-二甲基甲酰胺500ML69DT0152-4L N,N-二甲基甲酰胺4L592 DT0153-4L N,N-二甲基甲酰胺-HPLC4L0DT0153-500ML N,N-二甲基乙酰胺500ML84DT0163-500ML二甲亚砜500ML118 DT0164-4L二甲亚砜4L788 DT0746-500ML二氯甲烷500ML32DT0777-500ML N,N-二甲基甲酰胺500ML42DT0778-500ML N,N-二甲基乙酰胺500ML39DT0913-25G酚酞25G12DT0913-2KG酚酞-IND2KG0DT0973-25G二甲基黄25G38DT0994-25G变色酸钠25G42DT0997-25G二苯胺磺酸钠25G20DT1043-5G双硫腙(铅试剂)5G18DT1079-25G二苯偶氮碳酰肼25G38DT1100-250G对苯二酚250G37DT1101-100G间苯二酚100G26DT1102-100G邻苯二酚100G156 DT1321氯化钙干燥管,单球5个20DT1322氯化钙干燥管,双球5个20DT1568-500G硅藻土500G22DT1609-25G十二烷基-三甲基-溴化铵25G26DT1609-500G十二烷基-三甲基-溴化铵-AR500G0DT1612-100G十二烷基-三甲基-氯化铵-AR100G0DT1612-25G十二烷基-三甲基-氯化铵25G26DT1621-100G二苯胺100G19DT1621-2KG二苯胺-AR2KG0DT1635-100G对苯二胺100G32DT1635-25G对苯二胺25G0DT1636-100G邻苯二胺100G32DT1637-100G间苯二胺100G32DT1644-500ML二乙醇胺500ML29DT1664-500ML N,N-二甲基甲酰胺500ML34DT1665-500ML N,N-二甲基乙酰胺500ML32DT1697-500G月桂酸500G29DT1729-500G D-酒石酸500G104DT1731-100G DL-苹果酸-BR100G0DT1731-500G DL-苹果酸500G38DT1899-500G糊精500G29DT1976-100G2,4-二硝基苯酚100G44DT1977-100G二硝基苯酚100G64DT2003-500ML二氯甲烷500ML24DT2043-10G甜醇10G78DT2047-25G盐酸吡哆醇25G24DT5551-100G邻二氯苯酚100G97DTC003组织培养皿,12/包10*包26DTC007组织培养皿,10/包10包28DTC009组织培养皿,10/包10包35DTC015组织培养皿,单独包装包19E0105-100G乙二胺四乙酸二钠盐二水100GE0105-1KG乙二胺四乙酸二钠盐二水1KGE0105-500G乙二胺四乙酸二钠盐二水500GE0105-50KG EDTA DISODIUM SALTE0109-100G曙红Y100G378E0109-25G曙红Y25G104E0109-5G曙红Y5G32E0219-10G红霉素10G265E0219-2G红霉素2G63E0219-50G红霉素50G1102E0322-100G乙二胺四乙酸100G45E0322-1KG乙二胺四乙酸1KG361E0322-500G乙二胺四乙酸500G198E0492-1G溴化乙锭-7折优惠E0492-25G溴化乙锭-7折优惠E0492-500G ETHIDIUM BROMIDE[EB]500GE0492-5G溴化乙锭-7折优惠E0582-1L乙二醇1L212E0582-250ML乙二醇250ML65E0582-4L ETHYLENE GLYCOL4LE0720-100G乙二胺四乙酸二钾盐二水100GE0720-20KG EDTA DEPOTASSIUN SALTE0720-500G乙二胺四乙酸二钾盐二水500GE0732-1KG EGTA1KG0E0732-250G EGTA250G3236E0732-50G EGTA50G690E0732-5G EGTA5G83E261Low Range IE273Wide-Range Marker200μGE279Marks-IT TM100μGE683Low Range IIE687Low Range IIIE840DNA MicroMarker TME854PCR DNA MarkerEB0075-100G二乙胺四乙酸二钾盐二水100G0EB0075-500G二乙胺四乙酸二钾盐二水500G0EB0107-100G乙二胺四乙酸-7折优惠EB0107-1KG乙二胺四乙酸-7折优惠EB0107-500G乙二胺四乙酸-7折优惠EB0185-100G乙二胺四乙酸二钠盐二水-EB0185-1KG乙二胺四乙酸二钠盐二水-EB0185-500G乙二胺四乙酸二钠盐二水-EB0192-25G红霉素25G408EB0192-5G红霉素5G98EB0195-1G溴化乙锭-7折优惠EB0195-5G溴化乙锭-7折优惠EB0577-100G EDC100G0EB0577-1G EDC1G89EB0577-25G EDC25G202EB0577-5G EDC5G425EB315-100U无机焦磷酸酶-取消EB315-500U无机焦磷酸酶-取消EB333-200U无机焦磷酸酶-取消EB333-50U无机焦磷酸酶-取消EB517-10KU MMLV Reverse Transcriptase EB517-2KU MMLV Reverse Transcriptase EE406-5ML ETHIDIUM BROMIDE[EB]5MLEE522-100ML EDTA100ML192EF0261碱性磷酸酶,150U/μL7500U899EF0341碱性磷酸酶200U187EF0342碱性磷酸酶5*200U560EJ586-5MG E-645MG936EJ844-10TABS ETHIDIUM BROMIDE[EB]10TABSEJ844-25TABS ETHIDIUM BROMIDE[EB]25TABSEJ845-10T ETHIDIUM BROMIDE[EB]10TABSEJ845-25T ETHIDIUM BROMIDE[EB]25TABSEK0031T4多聚核苷酸激酶500UEK0032T4多聚核苷酸激酶2500UEL0011T4DNA连接酶EL0012T4DNA连接酶EL0013T4DNA连接酶EL0014T4DNA连接酶EL0017T4DNA连接酶EL0021T4RNA连接酶EL0331T4DNA连接酶EL0334T4DNA连接酶EL0337T4DNA连接酶ELS002290弹性蛋白酶25MG401ELS002292弹性蛋白酶100MG1269EN0171核酸酶BAL3150UEN0181微球菌核酸酶8000U455EN0191外切核酸酶III4000UEN0201核糖核酸酶H100UEN0202核糖核酸酶H500UEN0321核酸酶S110000U EN0361尿嘧啶DNA糖基酶200U455EN0362尿嘧啶DNA糖基酶5×200U1819EN0531RIBONUCLEASE A10MGEP0041DNA聚合酶IEP0042DNA聚合酶IEP0051DNA聚合酶IEP0052DNA聚合酶IEP0061T4DNA聚合酶EP0062T4DNA聚合酶EP0081T7DNA聚合酶EP0101T3RNA聚合酶EP0102T3RNA聚合酶EP0103T3RNA聚合酶EP0111T7RNA聚合酶EP0112T7RNA聚合酶EP0113T7RNA聚合酶EP0131SP6RNA聚合酶EP0161末端脱氧核苷转移酶500U606EP0162末端脱氧核苷转移酶2500U2425EP0421DNA polymerase IEP0422DNA polymerase IEP0441M-MLV逆转录酶2×5000U EP0442M-MLV逆转录酶10×5000U ER0011Alu I600UER0021Alw21I200UER0022Alw21I1000UER0031Alw26I1000UER0032Alw26I5000UER0041Alw44I1000UER0042Alw44I5000UER0051BamH I4000UER0052BamH I20000U ER0053BamH I HC20000U ER0061Bcn I1000U ER0062Bcn I5000U ER0072Bg1I10000U ER0081Bgl II500UER0082Bgl II2500U ER0091Bpu1102I200UER0092Bpu1102I1000U ER0111Bsp68I500UER0112Bsp68I2500U ER0121Bsp1191I2500U ER0131Bsp120I1500U ER0132Bsp120I7500U ER0133Bsp120I HC7500U ER0142Bsu15I3000U ER0151BsuR I3000U ER0152BsuR I15000U ER0161Cfr I200UER0162Cfr I1000U ER0172Cfr9I1500U ER0181Cfr10I200UER0182Cfr10I1000U ER0192Cfr13I5000U ER0202Cfr42I6000U ER0211Csp6I1500U ER0221Dra I1500U ER0222Dra I75000U ER0231Eam1104I300UER0232Eam1104I1500U ER0241Eam1105I1000U ER0242Eam1105I5000U ER0251Ecl136II1500U ER0252Ecl136II7500U ER0273EcoR I HC25000U ER0281Eco24I1500U ER0291Eco31I500UER0292Eco31I2500U ER0301Eco32I2000U ER0303Eco32I HC1000U ER0311Eco47I800UER0322Eco47III1000U ER0331Eco52I500UER0332Eco52I2500U ER0342Eco57I500UER0362Eco72I10000U ER0372Eco81I2500UER0381Eco88I1000U ER0392Eco91I5000U ER0401Eco105I600UER0402Eco105I3000U ER0421Eco147I1000U ER0422Eco147I5000U ER0432Sca I5000U ER0452Esp3I1000U ER0471Hin1I300UER0472Hin1I1500U ER0482Hin6I10000U ER0491Hinc II500UER0501Hind III5000U ER0502Hind III25000U ER0503Hind III HC25000U ER0512Hpa II5000U ER0521Kpn I4000U ER0522Kpn I20000U ER0523Kpn I HC20000U ER0541Msp I3000U ER0542Msp I15000U ER0551Mva I2000U ER0552Mva I10000U ER0562Mlu I5000U ER0571Nco I500UER0572Nco I2500U ER0581Nde I500UER0582Nde I2500U ER0591Not I300UER0601Pae I500UER0602Pae I2500U ER0611Pst I3000U ER0613Pst I HC15000U ER0622Pvu I1500U ER0631Pvu II2500U ER0632Pvu II12500U ER0633Pvu II HC12500U ER0641Sal I1500U ER0642Sal I7500U ER0643Sal I HC7500U ER0651Sdu I500UER0661Sma I1200U ER0662Sma I6000U ER0663Sma I HC6000U ER0671Taq I3000U ER0672Taq I15000U ER0681Xba I1500U。

压力容器专业词汇(英文)

压力容器专业词汇(英文)

压力容器专业词汇A B类Category A B安全阀Safety valve安装Installation鞍式支座saddle support凹面concave半球形封头Hemispherical heads棒bars保温支撑insulation support爆炸性explosive泵pump变径段reducers标记stamping标志marking标志的移植Transfering marking波纹板Corrugating Paper补强管reinforcing nozzle补强圈reinforcing ring不锈钢stainless steel不圆度out-of-roundness材料materials材料证明书Certification of material超声检验Ultrasonic Examination衬里Linings成型Forming成型封头Formed heads尺寸Dimensions翅片管finned tubes冲击试验impact test储罐storage tank传热面积heat transfer surface磁粉检验Magnetic Particle Examination次要应力secondary stress粗糙度roughness淬火Quenching带折边的锥形toriconical弹簧springs弹簧垫圈spring washer弹性模量modulus of Elasticity挡板baffle plate低合金钢容器low alloy steel vessels低温容器Low-temperature vessels地震烈度seismic intensity垫板Backing strip垫片gasket垫圈washer碟形封头Dished heads顶丝jbckscrew定距管spacer定位销pin dowel定义Definitions毒性toxicity镀锌容器Galvanized vessels锻件Forgings对焊法兰welding neck flange耳座lug阀门valves法兰flanges法兰接触面Flange contact facings防冲板impingement baffle防腐蚀衬里Corrosion resistant linings防火fire protection防涡流挡板vortex breaker非受压件nonpressure parts非圆形容器Noncircular vessels峰值应力peak stress腐蚀裕量corrosion allowance附加载荷supplementary loading附件attachments复合板Clad plate覆层容器clad vessels盖板Cover plates杆rods or bars过渡段transition in过渡圆角Knuckles焊后热处理after postweld heat treating焊接工艺welding procedure specification 焊接接头welding joints焊接系数welding coefficient厚度thickness滑动的sliding环向应力hoop stress回火tempering基本地震加速度basic seismic acceleration基本分压basic wind pressure计算厚度calculated thickness技术条件form of Specification加强圈stiffening rings夹套容器Jacketed vessels检查孔Inspection openings检验inspection角焊Fillet welds接地板earth lug截止Stop valves介质特性fluid property金属温度Metal temperature筋板rib plate径向应力radial stress静(压力)水头static head局部areas局部local局部焊后热处理Local postweld heat treatment 矩形设计rectangular design卡箍clamp开孔补强reinforcement for openings快开盖Quick-actuating closures拉杆tie rod裂缝Cracking流体(介质)fluid螺孔holes for screw螺母nut螺塞plug螺栓bolt螺纹threaded螺柱studs名义厚度nominal thickness铭牌Name plates内部构件Internal structures内衬筒internal shell盘管coil tube配件fittings膨胀节expansion joint平封头Flat heads评定Qualification气孔Porosity气压试验Pneumatic test钎焊brazing强度strength of球形封头spherically dished曲率curvature屈服yielding全容积total volume缺陷defects群座skirt support热处理thermal treatment热处理heat treatmen热应力thermal stress人孔manholes韧性ductility容积volume容器vessel容器净重empty weight容器类别vessel classification塞焊Plug welds设计压力design pressure射线超声检验Radiographic Examination渗透检验Penetrant Examination石墨graphite试样Test coupons适用范围Scope手孔(ha)ndholes水压试验hydrostatic test/hydraulic test/hydrotest 碳钢carbon steel搪玻璃容器enameled vessels梯子、平台ladders, platforms填充金属filler metal筒体shell凸面convex凸缘socket椭圆封头Ellipsoidal heads外压容器vessels subjected to external pressure 未注尺寸公差tolerance grade n0t noted无支撑unstayed系数fact0r现场安装Fie1d assemb1y校核checking 0f泄放(Disc)harge泄压装置Pressure relieving (dev)ices性能properties许用工作压力al1owable working pressure许用应力al1owable stress易然的flammable应力腐蚀stress corrosion应力集中stress concentration预热Preheating圆度roundness圆角和倒角corners+fillets载荷Loadingse(xpa)nded c0nnections折流板baffle plate蒸发器(Evap)orators直边长度length 0f skirt直径Diameter制造fabrication制造厂fabricator制造方法methods 0f fabrication制造工艺fabrication technology周长Girth主应力primary stress柱状壳体Cylindrical shells铸铁容器cast iron vessels转角半径knuckle radius装配assembling锥度tapered锥壳conical shell锥形封头Conical heads资格qualification纵向接头Longitudinal joints组对fitting up无损检测专业词汇a cluster of flaws 密集区缺陷A.C magnetic saturation 交流磁饱和ablution 清洗Absorbed dose rate 吸收剂量率Absorbed dose 吸收剂量Acceptanc limits 验收范围Acceptance level 验收水平验收标准acceptance specification 验收规范Acceptance standard 验收标准Accessories 附件配件辅助设备辅助器材Accumulation test 累积检测Accuracy 精确度准确度acetone 丙酮Acoustic emission (AE) 声发射Acoustic emission count 声发射计数Acoustic emission transducer 声发射换能器Acoustic holography 声全息术Acoustic impedance matching 声阻抗匹配Acoustic impedance method 声阻法Acoustic impedance 声阻抗Acoustic wave 声波Acoustical lens 声透镜Acoustic-ultrasonic 声-超声(AU)across 交叉横过Activation 活化Activity 活度additional stress 附加应力address: 地址:?Adequate shielding 适当防护、适当屏蔽AE 声发射air header 集气管"air set 空气中凝固, 常温自硬自然硬化" air supply 气源aisle 过道走廊alarm condition 报警状态alarm level 报警电平Alignment 对准定位调整校直alkaline battery 碱性电池allowable variation 允许偏差容许变化alternating current 交流电aluminum powder 铝粉amount 数量Ampere turns 安匝数amplifier panel 放大器面板Amplitude 振幅、幅度analyzer 分析器anchor bolt 锚定螺栓地脚螺栓Angle beam method 斜射法、角波束法Angle beam probe 斜探头、角探头angle fitting 弯头angle iron 角钢角铁Angle of incidence 入射角Angle of reflection 反射角Angle of spread 扩散角Angle of squint 偏向角、偏斜角Angle probe 斜探头、角探头angle square 角尺angle steel 角钢Angstrom unit 埃(长度单位) appearance 外观application drawing 操作图应用图arc cutting 电弧切割arc gouging 电弧刨削arc starting 起弧arc welding 电弧焊Area amplitude response curve 面积振幅响应曲线Area of interest 评定区、关注区域argon arc welding 氩弧焊Arliflcial disconlinuity 人工不连续性、人工缺陷arrangement diagram 布置图arrival time interval(Δtij)到达时间差(Δtij) Artifact 人为缺陷Artificial defect 人工缺陷A-scan A型扫描A-scope A型显示assembly 装配At present 目前Attenuation coefficient 衰减系数Attenuator 衰减器Audible leak indicator 音响泄漏指示器automatic temperature recorder 温度自动记录器Automatic testing 自动检测Autoradiography 自动射线照相术axiality 同轴度轴对称性axonometric drawing 轴测图back-feed 反馈background target 目标本底background 背景本底backwall echo 底波底面回波baiting valve 放料阀band plate 带板banjo fixing 对接接头Barium concrete 钡混凝土Barn 靶Base fog 片基灰雾base material 基底材料basic sensitivity 基准灵敏度Bath 槽液、浴池、槽Bayard-Alpert ionization gage B-A型电离计Beam angle 波束角、束张角Beam axis 声束轴线Beam path location 声程定位、声束路径位置Beam path 声程、声束路径Beam ratio 光束比Beam spread 声束扩散Beam 声束、光束bellow type 波纹管式bend 弯管弯头弯曲bending deformation 挠曲变形弯曲变形Bending 挠曲弯曲Betatron 电子感应加速器beveled edges 坡口beveling 磨斜棱磨斜边成斜角Bid 投标出价Bimetallic strip gage 双金属片计Bipolar field 双极磁场bisectrix 等分线Black and White Transmission Densitometer 黑白透射密度计Black light filter 黑光滤波器、黑光过滤片Black light 黑光,紫外光Blackbody equivalent temperature 黑体等效温度Blackbody 黑体Bleakney mass spectrometer 波利克尼质谱仪Bleedout 渗出、漏出blind plate 隔离盲板盲板blind 挡板窗帘blinding plate 盲板block up 封堵垫高blowhole 喷水孔通风孔通气孔(气)孔铸孔砂[气]眼气泡bolt 螺栓螺钉bolton 螺栓紧固bonding wire 接合线焊线boost pressure 升压both sides welding 双面焊接Bottom echo 底波底面回波bottom plate 底板Bottom surface 底面Boundary echo 边界回波、界面回波branch connection 分支接续分支连接Brand name 商标名称品牌breaking of contact 断接卡Bremsstrahlung 轫致辐射bridge 桥Broad-beam 宽射束宽(声、光)束Brush application 刷涂B-scan B型扫描B扫描B-scan presentation B型扫描显示B-scope B型显示buckle 满扣扣住buckling deformation 翘曲变形buried depth 埋深burn through 烧蚀烧穿bus duct 母线槽butt joint 对接butt jointing 对接接头butt weld 对接焊缝butt welding 对接焊cable armor 电缆铠装cable channel 电缆槽电缆管道cable fitting电缆配件cable gland 电缆衬垫cable laying 电缆敷设cable routing电缆路由选择Cable sheath 电缆包皮层cable testing bridge 电缆测试电桥cable tray 电缆盘cable trunk 电缆管道电缆主干线calculation sheet 计算书Calibrated Density Reference Scale 标准密度校验片Calibration instrument 校准仪器calorimeter 热量计Capillary action 毛细管作用Capping ends 顶盖末端carbon steel tube 碳钢管carbon steel 碳钢Carrier fluid 载液Carry over of penetrate 渗透剂转入cascade connection 串联连接Cassette 暗盒Cathode 阴极cathodic protection system 阴极保护系统caulking metal 填隙合金[金属](材料)CCD Camera 电荷耦合摄像机cement lined piping 水泥衬里管线center bearing bracket 中心支架center line 中心线center plate 中心板拨盘Central conductor method 中心导体法Central conductor 中心导体中线(三相四线制)centralized 集中的centripetal canting pull rope 向心斜拉索certification of fitness 质量合格证书channel bases沟渠基底channel steel expansion ring 槽钢胀圈Characteristic curve of film 胶片特性曲线Characteristic curve 特性曲线Characteristic radiation 特征辐射标识辐射Charge Coupled Device 电荷耦合器件(简称CCD)"check against 检查, 核对"check valve 止回阀Chemical fog 化学灰雾chipping 修琢chronometer精密计时表Ci 居里Cine-radiography 射线(活动)电影摄影术连续射线照相Circuit breaker断路开关circular array 圆形阵列Circumferential coils 圆环线圈Circumferential field 周向磁场环形磁场circumferential joint 周圈接缝Circumferential magnetization method 周向磁化法circumferential weld 环焊缝civil engineer 土木工程师civil works 土建工程建筑工程clamp 夹钳clamping fixture 胎具夹具Clean 清理Clean-up 清除Clearing time 定透时间coaxial cable 同轴电缆cockle stairs 螺旋梯Coercive force 矫顽力Coherence length 相干长度(谐波列长度)Coherence 相干性Coil method 线圈法Coil reference 线圈参考(参照线圈)Coil size 线圈尺寸Coil spacing 线圈间距Coil technique 线圈技术线圈法Coil test 线圈试验Coincidence discrimination 符合性鉴别一致性鉴别cold lap 冷隔Cold-cathode ionization gage 冷阴极电离计collar extension 环口Collimation 准直Collimator 准直器collision 碰撞冲突color identification 彩色识别Combined colour contrast and fluorescent penetrant 着色荧光渗透剂commencement 开始company 公司comply with 遵守component part 构件(组合)零件部件comprehensive analysis and judgement 综合分析判断Compressed air drying 压缩空气干燥compression joint 压接压力接合compression pump 压缩机压气机[泵]Compressional wave 压缩波Compton scatter 康普顿散射Computed Radiography(CR)计算机辅助射线成像技术condensation 冷凝conducting wire 导线conductive paste 导电膏conduit box [电]导管分线匣conduit entry 导管引入装置conduit outlet 电线引出口connector 接线器连接器Console 控制台construction work 施工工程consumable insert (焊接)自耗嵌块consumer 用户Contact pads 接触垫contactor 触头接触器触点开关content gauge 液位计Continuous emission 连续发射Continuous linear array 连续线性阵列Continuous method 连续法Continuous spectrum 连续谱Continuous wave 连续波Continuously Welded (CW) 连续焊Contrast agent 对比剂造影剂Contrast aid 反差增强剂Contrast sensitivity 对比灵敏度Contrast stretch 对比度扩展Contrast 对比度衬度control cable控制电缆操纵索control console 控制台Control echo 控制回波control unit 控制单元control valve actuator 阀控传动机构control valve控制阀control wiring 控制线路control 控制控制器convenience receptacle 电源插座convexity 凸面Copper intensifying screens 铜增感屏core rod 芯棒corresponding 相应的cotton fibre 棉质纤维Couplant 耦合剂Coupling losses 耦合损失Coupling medium 耦合介质Coupling 耦合CR(Computed Radiography)计算机辅助射线成像技术Cracking 破裂裂纹裂化裂解crate 板条箱柳条箱crater crack (焊接)弧坑裂纹Creeping wave 爬波蠕变波Critical angle 临界角Cross section 横截面Cross talk 串音Cross-drilled hole 横孔crossed yoke 交叉磁轭Crystal 晶片晶体C-scan C型扫描C扫描C-scope C型显示C-shape detector C型探测器(X射线实时成像)"cubicle 室,箱"Curie point 居里点Curie temperature 居里温度Curie(Ci) 居里current attenuation 电流衰减Current flow method 通电法电流法Current induction method 电流感应法Current magnetization method 电流磁化法cushion 垫层衬垫Cut-off level 截止电平cutting opening 切孔切开Cutting 切割CW (Continuously Welded) 连续焊Dark room packing 暗室包装(在暗室条件下将X射线胶片装入暗盒)data logger 数据记录器datum mark 基准点Dead zone 盲区死区Decay curve 衰变曲线Decibel(dB) 分贝Defect detection sensitivity 缺陷探测灵敏度defect evaluation zone 缺陷评定区Defect resolution 缺陷分辨力Defect 缺陷Definition 清晰度定义delivery 发货Demagnetization factor 退磁因子退磁系数Demagnetization 退磁Demagnetizer 退磁装置退磁器Densitometer 黑度计密度计Density comparison strip 黑度比较片密度比较条Density Strip 黑度比较片密度比较条Density 黑度(底片)密度depth scan 深度扫描description 说明描述design pressure 设计压力Detecting medium 检验介质Detergent remover 洗净液去垢剂Developer aqueous 水性显像剂Developer dry 干式显象剂Developer liquid film 液膜显象剂Developer nonaqueous(suspend)非水(悬浮)显象剂Developer station 显像工位显影台Developer 显像剂显影剂Developing time 显像时间显影时间Development 显影differential discriminator (电子)差动式鉴频器Diffraction mottle 衍射斑点衍射斑纹Diffuse indications 扩散指示Diffusion 扩散漫射digital detector 数字探测器(X射线实时成像)Digital display 数字显示数显Digital image acquisition system 数字图像采集系统Digital Radiography(DR)数字射线成像技术Digital Thermometer 数字温度计(用于测定胶片处理液的温度)Digital timer for darkroom 暗室用计时器(在暗室中可调及报警)Dilatational wave 膨胀波疏密波dimensional inspection 尺寸检验Dip and drain station 浸渍和流滴工位浸渍与滴落台Direct contact magnetization 直接接触磁化Direct contact method 直接接触法Direct exposure imaging 直接曝光成像directional beam 定向辐射(指定向辐射的工业X射线机)Directivity 指向性disassembly and assembly 拆装Discontinuity 不连续性Distance marker 距离标志Distance-gain-size DGS曲线(距离-增益-尺寸曲线)(DGS德文为A VG)"distribution board 配电盘, 配电屏"Dose equivalent 剂量当量Dose rate meter 剂量率计dose 剂量Dosemeter 剂量计dosimeter 剂量计Double crystal probe 双晶探头Double probe technique 双探头法Double transceiver technique 双发双收法Double traverse technique 双光路技术doubleskin 重皮down lead 引下线DR(Digital Radiography)数字射线成像技术Dragout 废酸洗液Drain time 滴落时间排液时间drain 排水管排水沟排水道排水draught 气流Drift 漂移Dry developer 干式显像剂Dry developing cabinet 干式显像柜Dry method 干法Dry powder 干粉Dry technique 干法Drying oven 干燥箱干燥炉Drying station 干燥工位干燥台Drying time 干燥时间drying 烘干D-scan D型扫描D-scope D型显示dual element transducers 双晶探头双晶片换能器Dual search unit 双探头双探测装置双探测器Dual-focus tube 双焦点(X射线)管due date 到期日预定日期Duplex wire type Image Quality Indicator 双线型像质指示器双线像质计Duplex-wire image quality indicator 双线像质指示器双线像质计duplicate part 备件Duration 持续时间Dwell time 停留时间dye penetrant examination 着色渗透检验Dye penetrant 着色渗透剂Dynamic leak test 动态泄漏检测Dynamic leakage measurement 动态泄漏测量Dynamic radiography 动态射线照相法Dynamic range 动态范围earth resistance 接地电阻earth wire 接地线地线earthing device 接地装置earthing pole 接地极Echo frequency 回波频率Echo height 回波高度Echo indication 回波指示Echo transmittance of sound pressure 声压往复透过率Echo width 回波宽度Echo 回波echodynamic patterns 回波动态波型eddy current coil 涡流检测线圈Eddy current flaw detector 涡流探伤仪eddy current probe 涡流检测探头Eddy current testing 涡流检测Eddy current 涡流涡电流Edge echo 棱边回波Edge effect 边缘效应Edge 边缘棱边Effective depth penetration (EDP)有效穿透深度有效透入深度Effective focus size 有效焦点尺寸Effective magnetic permeability 有效磁导率Effective permeability 有效磁导率有效渗透率相对渗透率Effective reflection surface of flaw 缺陷有效反射面Effective resistance 有效电阻Elastic medium 弹性介质elbow 弯管接头管肘Electric displacement 电位移electric force compounded grease 电力复合脂electric heat tracing 电伴随加热electric machine 电机electric pressure 电压electrical appliance 电器Electrical center 电中心electrical material电气材料"electrical panel 配电板,配电盘"electrified 带电Electrode 电极电焊条Electrolytic Sliver recovery unit 电解银回收装置(从定影液中回收银)Electromagnet 电磁铁Electro-magnetic acoustic transducer 电磁声换能器Electromagnetic induction 电磁感应Electromagnetic radiation 电磁辐射Electromagnetic testing 电磁检测Electro-mechanical coupling factor 机电耦合系数electron linear accelerator 电子直线加速器Electron radiography 电子辐射照相术Electron volt 电子伏恃Electronic linear scans (E-scans) 电子线性扫描Electronic noise 电子噪声Electronic scanning (E-scans) 电子扫描(E-扫描)Electrostatic spraying 静电喷涂E-mail: 电子邮件:? 电子邮箱:embedded part 预埋件嵌入[埋置]部分emission count (声)发射计数Emulsification time 乳化时间Emulsification 乳化Emulsifier 乳化剂Encircling coils 环形线圈End effect 端部效应端点效应"end socket 端头, (钢索的)封头"Energizing cycle 激励周期enfoldment 折迭envelope 包络包迹environment visible light 环境可见光Equalizing filter 均衡滤波器平衡滤波器equipment 器材设备Equivalent I.Q.I. Sensitivity 当量象质指示器灵敏度Equivalent method 当量法Equivalent nitrogen pressure 等效氮压当量氮气压力Equivalent penetrameter sensifivty 当量透度计灵敏度Equivalent 当量Erasabl optical medium 可消光介质erection 架设Etching 浸蚀腐蚀侵蚀蚀刻Evaluation threshold 评价阈值评定阈Evaluation 评定Event count rate 事件计数率Event count 事件计数Examination area 检验范围Examination region 检验区域examination 试验检验考试examine and approve 审批exfoliation 剥落脱落Exhaust pressure/discharge pressure 排气压力/排放压力出口压力输送压力Exhaust tubulation 排气管道Expanded time-base sweep 时基线展宽时基扫描扩展"expansion bolt 伸缩栓, 扩开螺栓"expansion joint 伸缩接头Exposure and darkroom accessories 曝光与暗室附件Exposure chart 曝光曲线Exposure fog 曝光灰雾Exposure table 曝光表Exposure 曝光Exposure 曝光Extended source 扩展源延长源external diameter of the pipe 管子直径external diameter 外径extra fee 额外费用?eye survey 目测fabrication drawing 制造图纸制作图fabrication tolerance 制造容差fabrication 加工制造Facility scattered neutrons 易散射中子fall off 脱落False indication 假指示虚假指示伪显示虚假显示Family 族系列Far field 远场Fast neutron detectors 快中子探测器Fast/slow timing Oscilloscope 带快慢调速的示波器Fax: 传真:?FBH 平底孔(缩写)feeder 馈电线Feed-through coil 穿过式线圈ferritic 铁素体的field fabricated 工地制造的现场装配的field installation 现场安装field instrument 携带式仪表Field 场(磁场、声场)filament 灯丝Fill factor 填充系数filler metal 焊料焊丝filler rod 焊条fillet weld 角焊填角焊filling water test 充水试验Film badge 胶片剂量计Film base 片基film cassette 胶片暗盒Film contrast 胶片对比度film density 胶片密度,底片黑度film evaluation scope 底片评定范围Film gamma 胶片γ值(胶片灰度系数)Film Hangers (Channel Type)槽式洗片架(手工洗片的一种洗片架类型)Film Hangers (Clip Type)夹式洗片架(手工洗片的一种洗片架类型)Film Hangers for manual processing 洗片架(手工洗片的胶片挂架)Film marking equipment 胶片标记器材Film processing chemicals 胶片处理药品洗片药品Film processing 胶片冲洗加工底片处理Film speed 胶片速度(胶片感光速度胶片感光度)Film unsharpness 胶片不清晰度film viewer 底片评片灯观片灯底片观察用光源Film viewing equipment 评片装置(观片灯)Film viewing screen 胶片观察屏Filter 滤波器滤光板过滤器Final test 最终检验fire barriers 防火间隔防火屏障Fixing 固定flange connection 凸缘联接flange gasket 法兰垫片flange joint 凸缘接头flange sealing surface 法兰密封面flange 边缘轮缘凸缘法兰flash plate 闪熔镀层flash point 闪点Flat-bottomed hole equivalent 平底孔当量Flat-bottomed hole 平底孔Flaw characterization 伤特性缺陷特征Flaw echo 缺陷回波flaw height(thru-wall dimension) 缺陷自身高度(缺陷在壁厚方向的尺寸)Flaw 伤缺陷瑕疵裂纹flexible conduit 软管Flexural wave 弯曲波flicker-free images 无闪烁图像Floating threshold 浮动阀值floor slab 楼板flow instrument 流量计flow sheet 流程图Fluorescence 荧光Fluorescent dry deposit penetrant 干沉积荧光渗透剂Fluorescent examination method 荧光检验法Fluorescent light 荧光荧光灯Fluorescent magnetic particle inspection 荧光磁粉检验Fluorescent magnetic powder 荧光磁粉Fluorescent penetrant 荧光渗透剂Fluorescent screen 荧光屏fluorography 荧光照相术Fluorometallic intensifying screen 荧光金属增感屏Fluoroscopy 荧光检查法flushing 冲洗填缝flux cored arc welding 带焊剂焊丝电弧焊Flux leakage field 磁通泄漏场漏磁场Flux lines 磁通线flux 焊剂,熔化Focal distance 焦距Focal spot 焦点Focus electron 电子焦点聚焦电子束Focus length 焦点长度聚焦长度Focus size 焦点尺寸聚焦尺寸Focus width 焦点宽度聚焦宽度Focused beam 聚焦束(声束、光束、电子束)Focusing probe 聚焦探头Focus-to-film distance(f.f.d) 焦点-胶片距离(焦距)Fog density 灰雾密度Fog 灰雾Footcandle 英尺烛光formula 公式foundation ring 底圈foundation settlement 基础沉降Freguency 频率Frequency constant 频率常数Fringe 干涉带干扰带条纹边缘Front distance of flaw 缺陷前沿距离Front distance 前沿距离full-scale value 满刻度值Full-wave direct current(FWDC)全波直流Fundamental frequency 基频Furring 毛状迹痕毛皮fusion arc welded 熔弧焊fusion 熔融熔合gage glass 液位玻璃管Gage pressure 表压表压力Gain 增益gamma camera γ射线照相机(γ射线探伤机)gamma equipment γ射线设备Gamma radiography γ射线照相术Gamma ray source container γ射线源容器Gamma ray source γ射线源Gamma rays γ射线gamma source γ射线源γ源Gamma-ray radiographic equipment γ射线照相装置Gamme ray unit γ射线机γ射线装置Gap scanning 间隙扫查间隙扫描gas cutting 气割gas shielded arc welding 气体保护焊Gas 气体Gate 闸门Gating technique 选通技术脉冲选通技术gauge board 仪表板样板模板规准尺Gauss 高斯Geiger-Muller counter 盖革.弥勒计数器generating of arc 引弧Geometric unsharpness 几何不清晰度girth weld 环形焊缝gland bolt 压盖螺栓Goggles 护目镜gouging 刨削槽Gray(Gy) 戈瑞Grazing angle 掠射角切线角入射余角Grazing incidence 掠入射切线入射grind off 磨掉grinder 磨床磨工grinding wheel 砂轮片砂轮groove face (焊缝)坡口面grounding conductor 接地导体Group velocity 群速度grouting 灌(水泥)浆"guide wire 尺度[定距]索,准绳""gusset plate角撑板, 加固板"Half life 半衰期Half-value layer(HVL) 半值层半价层Half-value method 半波高度法半值法Half-wave current (HW)半波电流Halogen leak detector 卤素检漏仪Halogen 卤素hanger 吊架挂架洗片架Hard hat 安全帽Hard X-rays 硬X射线Hard-faced probe 硬膜探头硬面探头Harmonic analysis 谐波分析Harmonic distortion 谐波畸变Harmonics 谐频谐波head face 端面Head wave 头波heat absorbing glass 吸热玻璃heat affected area 热影响区heat exchangers 热交换器heat transfer 热传输Heating boxes 加热箱Helium bombing 氦轰击法Helium drift 氦漂移Helium leak detector 氦检漏仪Hermetically tight seal 气密密封密封密封装置High energy X-rays 高能X射线high frequency generator 高频发电机high frequency 高频High vacuum 高真空highly sophisticated image processing 高度完善的图像处理hoisting upright column 吊装立柱Holography 全息照相术(光全息、声全息)horizontal line 水平线hydrophilic emulsifier 亲水乳化剂Hydrophilic emulsifier 亲水性乳化剂Hydrophilic remover 亲水性洗净剂亲水性去除剂hydrostatic pressure test静水压试验Hydrostatic test 水压试验静水压试验Hysteresis 磁滞滞后I.F. (intermediate frequency) 中频(30~3000千周/秒)IACS =International Annealed Copper Standard 国际退火(软)铜标准ice chest 冰箱ice machine 制冰机,冷冻机ice 冰iconoscope 光电摄像管ID (①inside ②inside dimensions) ①内径②内部尺寸ID coil ID线圈=Inside Diameter 内径线圈idea 概念,意见,思想ideal 理想的,想象的identical 同一的,恒定的,相同的identification mark 识别标志identification 鉴定,辨别,验明identifier 鉴别器identify pulse 识别脉冲idiopathetic 自发的,特发的IEM (ion exchange membrane) 离子交换膜illuminance 照(明)度illuminant 照明的,发光的illuminating lamp 照明灯泡illuminating loupe 放大照明镜illuminating mirror 照明镜illumination apparatus 照明器illumination plate 照明板illumination 照明的,照射illuminator ①照明器,照明装置②反光镜illuminometer 照度计illustration(abbr. illus.) 图解,例证,具体说明image amplifier 图像放大器,影像增强器image analysis system 图像分析系统Image contrast 图像对比度影像对比image converter 影像转换器Image definition 图像清晰度Image enhancement 图像增强image freeze 影像冻结image intensifier tube 影像增强管图像增强管image intensifier 像亮化器,图像增强器Image magnification 图像放大image monitor 图像监视器image multiplier 影像倍增器image pick-up tube 摄像管image quality indication 像质指示Image Quality Indicator (IQI)像质计像质指示器Image quality indicator sensitivity 像质指示器灵敏度Image quality 图像质量image reproducer 显像管,显像器image store 图像存储器image tube 显像管imager 图像仪,显像仪imagination 想象imagine 想象,推测,设想Imaging line scanner 图像线扫描器图像行扫描器Imaging Plates(IP)成像板immediate payment 立即付款Immersion probe 液浸探头Immersion rinse 浸没清洗浸液清洗immersion system 浸渍装置液浸系统Immersion testing 液浸试验Immersion time 浸没时间浸入时间immersion 浸没,浸渍impact strength 冲击强度impacter 冲击器impedance matching 阻抗匹配Impedance plane diagram 阻抗平面图impedance transducer 阻抗传感器,阻抗换能器Impedance 阻抗impeller ①叶轮,转子②压缩机Imperfection 不完整性缺陷imperial gallon(abbr. ip gal) 英国标准加仑(英制容量单位合4.546升)imperial quart 英制夸脱import ①进口②输入import and export firm 进出口商行import border station 进口国境站名import licence position 进口许可证importation ①输入,传入②进口货impression technic 印模术impression tray 印模盘impression 压迹,印模,版Impulse eddy current testing 脉冲涡流检测impulse generator 脉冲发生器impulse oscilloscope 脉冲示波器impulse recorder 脉冲自动记录器impulse scaler 脉冲计数器impulse timer 脉冲计数器impulse transmitting tube 脉冲发射管impulse 冲动,搏动,脉冲impulser 脉冲发生器,脉冲传感器impurity 不纯,杂质In (①indium ②inch) ①铟②英寸(等于25.4毫米)in parallel 并联in phase 同相的in series 串联in toto 全,整体in vacuo 在真空中inaction 无作用inadequacy 机能不全,闭锁不全Inc. (incorporated) 股份有限公司incandescent lamp 白炽灯inch(abbr.In;in) 英寸incidence ①入射,入射角②发生率incident angle 入射角incident illumination 入射光incident light 入射光incident ray 入射光incidental 偶发的,非主要的inclination 倾斜,斜度inclined tube type manometer 斜管式压力计include 包括,计入inclusion 包含包埋杂质incoming line 进线口incompatible 不相容的,禁忌的incompetence 机能不全,闭锁不全inconvertible 不可逆的incorporation ①并入②公司increase 增加,增大,增长Incremental permeability 增量磁导率indent 订单index ①指数,索引②指针index card 索引卡片index hand 指针index signal 指示信号indexer 指数测定仪,分度器indicate 指示,表明Indicated defect area 缺陷指示面积Indicated defect length 缺陷指示长度indicated light 指示灯indicating bell 指示铃indicating lamp 指示灯Indication 指示indicator ①指示器,显示器②指针③指示剂indicator paper 试纸indicatrix 指示量,指示线,特征曲线indifferent electrode 无关电极indiffusible 不扩散的indirect export 间接出口Indirect exposure 间接曝光indirect import 间接进口Indirect magnetization method 间接磁化法Indirect magnetization 间接磁化Indirect scan 间接扫查indium(abbr.In) 铟individual 个体的,个别的indoor 室内的induce 引起,感应,诱导Induced current method 感应电流法induced electricity 感生电,感应电Induced field 感应磁场感生场induct 感应,引导,引入inductance bridge flowmeter 感应电桥流量计inductance meter 电感测定计inductance 电感,感应系数induction ①引导,前言②感应,电感③吸气induction apparatus 感应器induction coil 感应线圈inductive transducer 感应传感器inductogram X射线照片inductometer 电感计inductor 感应器,感应机inductorium 感应器inductosyn 感应式传感器industrial exhibition 工业展览会industrial radiographic film dryer 工业射线胶片干燥器industrial robot 工业机器人Industrial X-ray films 工业X射线胶片industrial(abbr.indust.) 工业的,产品的industry 工业,产业indutrial X-ray machin 工业X射线机inert 惰性的,无效的inference 推论,推断infinite 无限的,无穷的infinitesimal 无限小的,无穷小的infinity 无穷大,无限,无限距inflame 燃,着火inflammable 可燃的,易燃的inflation 膨胀,充气,打气inflator 充气机inflow 流入,吸入,进气influence 影响,感应influx 流入,注入inform 报告,通告,告诉information generator 信息发送器information storage unit 信息存储器information 情报,资料,消息,数据infra- 下,低于,内,间infranics 红外线电子学infrared ①红外线的②红外线infrared detector 红外线探测器infrared drier 红外线干燥器infrared equipment 红外线设备infrared furnace 红外线炉infrared gas analyzer 红外线气体分析仪infrared heater 红外线加热器Infrared imaging system 红外成象系统infrared lamp 红外线灯infrared laser 红外激光器infrared light 红外线infrared liner polarizer 红外线直线偏振镜infrared photography 红外摄影术infrared radiation 红外线照射infrared radiator 红外线辐射器infrared rays 红外线Infrared sensing device 红外传感装置infrared spectrophotometer 红外分光光度计infrared thermography 红外热成象红外热谱infrasonic frequency 次声频infrequent 稀有的,不常见的Inherent filtration 固有滤波Inherent fluorescence 固有荧光inherent 生来的,固有的,先天的inheritance 遗传,继承inhibition 抑制,延迟,阻滞inhibitor 抑制剂抑制器inhomogeneous 不纯的,不均匀的in-house 自身的,内部的initial charge 起始电荷initial data 原始数据Initial permeability 起始磁导率初始磁导率Initial pulse width 始波宽度始脉冲宽度Initial pulse 始脉冲initial 开始的,最初的initiator ①创造人②引发剂injection syringe 注射器injection 注射,喷射injector pump 注射泵injector 注射器,喷射器injury 伤,损伤,损害ink jet printer 墨水喷射印刷机,喷水式打印机ink jet recorder 墨水喷射记录器ink writer 印字机ink writing oscillograph 记录示波仪ink 墨水,油墨inlay 嵌体,嵌入inlead 引入线inlet port 入口inlet 入口,入线,输入inner 内部的innocuous 无害的,良性的innovation 革新,改革innumerable 无数的,数不清的ino- 纤维inoperative 无效的,不工作的inorganic chemistry 无机化学inorganic 无机的inosculation 吻合,联合in-out box 输入-输出盒input buffer 输入缓冲器input coupler 输入耦合器input device 输入装置input filter 输入滤波器input impedance 输入阻抗input output adapter 输入-输出衔接器input tranformer 输入变压器input 输入,输入电路inscription 标题,注册insert ①插入物,垫圈②插入,植入insert tube 嵌入式X射线管Inserted coil 插入式线圈inserter 插入器,插入物insertion 插入inset 插页,插图,插入Inside coil 内部线圈inside 内部,内侧,在……里面Inside-out testing 外泄检测泄出检测insignificant 无意义的,轻微的insolation 曝晒,日照insoluble 不溶解的inspection certificate 检验证明书inspection fee 检验费Inspection frequency 检测频率inspection machine 检验设备Inspection medium 检查介质检验介质inspection standard 检验标准Inspection 检查检验inspection 验收,检查,商检inspector ①测定器②检验员inspectoscope 检查镜inspissator 浓缩器,蒸浓器instability 不稳定性install 安装,装置installation ①安装②装置,设备installation fundamental circle 安装基准圆。

尼罗红荧光染色溶液产品说明书(中文版)【模板】

尼罗红荧光染色溶液产品说明书(中文版)【模板】

尼罗红荧光染色溶液产品说明书(中文版)主要用途尼罗红荧光染色溶液是一种旨在通过与脂类物质的结合并发出荧光检测信号,快速、敏感、可靠地活体定量测定细胞内脂类成分的常用荧光染料。

适用于各种细胞包括细菌细胞,也用于蛋白质电泳染色。

产品严格无菌,即到即用,性能稳定,着色清晰灵敏。

技术背景尼罗红染色剂(9-diethylamino-5H-benzo[alpha]-phenoxazine-5-one;Nile red)是一种亲脂性的恶嗪类荧光染料。

与脂类物质包括腊酯(wax ester)和三酰甘油(triacylglycerol)以及各种脂肪酸结合后,在激发波长543nm的激发下,显示强烈桔红色荧光(散发波长598nm)。

同时在紫外光的照射下显示红色。

产品内容染色液(Reagent A)(0.5毫克/毫升)毫升产品说明书1份保存方式保存染色液(Reagent A)在-20℃冰箱里,避免光照,有效保证6月用户自备HANK平衡盐缓冲溶液(12028)或PBS缓冲溶液(12033):用于清理细胞胰蛋白酶乙二胺四乙酸混合液(12024):用于细胞脱离完全细胞培养液(12052):用于细胞处理所需的培养基15毫升锥形离心管:用于细胞收集的存放微型台式离心机:用于细胞沉淀收集台式离心机:用于细胞沉淀收集1.5毫升离心管:用于细胞检测操作的容器2毫升离心管:用于染色工作液配制的容器37℃培养箱:用于孵育反应物比色皿:用于荧光定量分析荧光显微镜:用于观察荧光细胞荧光分光光度仪:用于定量检测荧光细胞和脂类产量方法一、间接法实验开始前,将试剂盒里的染色液(Reagent A)冻融,置入冰槽里。

然后进行下列操作。

1.开启荧光显微镜或荧光分光光度仪2.小心抽掉25cm2细胞培养瓶里的培养液3.加入3毫升用户自备的HANK平衡盐缓冲溶液或PBS缓冲溶液到细胞培养瓶,覆盖培养瓶表面4.小心抽掉清洗液5.加入1毫升用户自备的胰蛋白酶乙二胺四乙酸混合液,铺满整个培养平面6.置入37℃培养箱1分种7.振动培养瓶,使细胞脱落8.加入5毫升用户自备的完全细胞培养液9.移入15毫升锥形离心管10.放进台式离心机离心10分钟,速度为300g11.小心抽去上清液12.加入1毫升用户自备的HANK平衡盐缓冲溶液或PBS缓冲溶液,充分混匀13.移入到新的1.5毫升离心管14.加入xx微升染色液(Reagent A)到离心管15.用手指轻轻弹动离心管,使其充分混匀16.在37℃培养箱孵育10分钟,避免光照17.放进微型台式离心机离心30秒,速度为16000g(或13000RPM,例如eppendorf 5415)18.小心抽去上清液19.加入1毫升用户自备的HANK平衡盐缓冲溶液或PBS缓冲溶液,充分混匀20.(选择步骤)放进微型台式离心机离心30秒,速度为16000g(或13000RPM,例如eppendorf 5415)21.(选择步骤)小心抽去上清液22.(选择步骤)加入1毫升用户自备的HANK平衡盐缓冲溶液或PBS缓冲溶液,充分混匀23.检测方法A)荧光显微镜定性分析1)移出10微升离心管中的混匀物到载玻片上2)放上盖玻片或封片3)在荧光显微镜下观察荧光细胞:激发波长543nm,散发波长598nm――显示强烈桔红色荧光细胞的为脂类丰富的阳性细胞B)荧光分光光度计定量分析1)移取1毫升细胞悬液到1毫升比色皿2)放进荧光分光光度计测读:激发波长543nm,散发波长598nm――确定活体细胞脂类产量方法二:直接法实验开始前,将试剂盒里的染色液(Reagent A)冻融,然后移出2毫升用户自备的HANK平衡盐缓冲溶液或PBS缓冲溶液到2毫升离心管,加入xx微升染色液(Reagent A),混匀后,置入冰槽里,标记为染色工作液。

美国肯纳金属样本

美国肯纳金属样本

Grain size < 0,2 μm 0,2 - 0,5 μm 0,5 - 0,8 μm 0,8 - 1,3 μm 1,3 - 2,5 μm 2,5 - 6,0 μm > 6,0 μm
German designation Nano Ultrafein Feinst Fein Mittel Grob Extragrob
中国总部 北京市朝阳区雅宝路10号凯威大厦1203室 电话: 010-85634883 上海市浦东金桥出口加工区金豫路750号 电话: 021-38608288 全面服务热线: 400 6501 388

工程产品 硬质合金棒料 旋转切削工具解决方案
硬质合金棒料Biblioteka 实心未精磨棒料 Ø 1,2 - 32,3 mm, length 325 + 10 mm ........................................................ 2
公司 全球总部 美国宾夕法尼亚州、拉特罗布15650 Technology路 1600号 电话: 800 446 7738 (美国与加拿大) 电子信箱: ftmill.service@
欧洲总部 Rheingoldstrasse 50 CH 8212 Neuhausen am Rheinfall 瑞士 电话: 0041 52 6750 100 电子信箱: @

3475525

3363307

3363308

3363309

3363313

3363314

3363315

3363064

3363068

3363071

ASTM标准中英文对照

ASTM标准中英文对照
标准号
A1-00 A2-02 A3-01 A6/A6M-04a A20/A20M-04a A27/A27M-03 A29/A29M-04 A31-04 A34/A34M-01 A36/A36M-04 A47/A47M-99 A48/A48M-03 A49-01 A53/A53M-04 A65-01 A66-01 A67-00 A74-04 A82-02 A90/A90M-01 A99-03 A100-04 A101-04 A102-04 A105/A105M-03 A106/A106M-04a A108-03 A109/A109M-03 A111-99a(2004)e1 A116-00 A121-99(2004) A123/A123M-02 A125-96(2001) A126-04 碳素钢丁字轨
A179/A179M-90a(2001) 热交换器和冷凝器用无缝冷拉低碳钢管 A181/A181M-01 A182/A182M-02 A183-03 A184/A184M-01 A185-02 A192/A192M-02 A193/A193M-04b A194/A194M-04a A197/A197M-00 A202/A202M-03 普通锻制碳素钢管的规格 高温设备用锻制或轧制的合金钢管法兰、锻制管件、阀门及 零件 钢轨用碳素钢螺栓和螺母 混凝土加筋用变形钢筋编织网 钢筋混凝土用焊接钢丝结构 高压用无缝碳素钢锅炉管 高温设备用合金钢和不锈钢螺栓材料 高温和高压设备用碳素钢与合金钢螺栓和螺母的规格 化铁炉用可锻铸铁 压力容器用铬锰硅合金钢板
英文名称
Standard Specification for Carbon Steel Tee Rails Standard Specification for Carbon Steel Girder Rails of Plain, Grooved, and Guard Types Standard Specification for Steel Joint Bars, Low, Medium, and High Carbon (Non-Heat-Treated) Standard Specification for General Requirements for Rolled Structural Steel Bars, Plates, Shapes, and Sheet Piling Standard Specification for General Requirements for Steel Plates for Pressure Vessels Standard Specification for Steel Castings, Carbon, for General Application Standard Specification for Steel Bars, Carbon and Alloy, Hot-Wrought, General Requirements for Standard Specification for Steel Rivets and Bars for Rivets, Pressure Vessels Standard Practice for Sampling and Procurement Testing of Magnetic Materials Standard Specification for Carbon Structural Steel Standard Specification for Ferritic Malleable Iron Castings Standard Specification for Gray Iron Castings Standard Specification for Heat-Treated Carbon Steel Joint Bars, Microalloyed Joint Bars, and Forged Carbon Steel Compromise Joint Bars Standard Specification for Pipe, Steel, Black and Hot-Dipped, ZincCoated, Welded and Seamless Standard Specification for Steel Track Spikes Standard Specification for Steel Screw Spikes Standard Specification for Steel Tie Plates, Low-Carbon and High-Carbon Hot-Worked Standard Specification for Cast Iron Soil Pipe and Fittings Standard Standard Articles Standard Specification for Steel Wire, Plain, for Concrete Reinforcement Test Method for Weight [Mass] of Coating on Iron and Steel with Zinc or Zinc-Alloy Coatings Specification for Ferromanganese

CellTiter Glo Luminescent Cell Viability Assay Protocol

CellTiter Glo Luminescent Cell Viability Assay Protocol

Promega Corporation ·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·1.Description (1)2.Product Components and Storage Conditions (4)3.Performing the CellTiter-Glo ®Assay (5)A.Reagent Preparation (5)B.Protocol for the Cell Viability Assay (6)C.Protocol for Generating an ATP Standard Curve (optional) (7)4.Appendix (7)A.Overview of the CellTiter-Glo ®Assay..............................................................7B.Additional Considerations..................................................................................8C.References............................................................................................................11D.Related Products. (12)1.DescriptionThe CellTiter-Glo ®Luminescent Cell Viability Assay (a–e)is a homogeneous method to determine the number of viable cells in culture based on quantitation of the ATP present, which signals the presence of metabolically active cells. The CellTiter-Glo ®Assay is designed for use with multiwell-plate formats, making it ideal for automated high-throughput screening (HTS) and cell proliferation and cytotoxicity assays. The homogeneous assay procedure (Figure 1) involves adding a single reagent (CellTiter-Glo ®Reagent) directly to cells cultured in serum-supplemented medium. Cell washing, removal of medium or multiple pipetting steps are not required.The homogeneous “add-mix-measure” format results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present (Figure 2).The amount of ATP is directly proportional to the number of cells present in culture in agreement with previous reports (1). The CellTiter-Glo ®Assay relies on the properties of a proprietary thermostable luciferase (Ultra-Glo™ Recombinant Luciferase), which generates a stable “glow-type” luminescent signal and improves performance across a wide range of assay conditions. The luciferase reaction for this assay is shown in Figure 3. The half-life of the luminescent signal resulting from this reaction is greater than five hours (Figure 4). This extended half-life eliminates the need for reagent injectors and provides flexibility for continuous or batch-mode processing of multiple plates. The unique homogeneous format reduces pipetting errors that may be introduced during the multiple steps required by other ATP-measurement methods.CellTiter-Glo ®Luminescent Cell Viability AssayAll technical literature is available on the Internet at: /protocols/ Please visit the web site to verify that you are using the most current version of this Technical Bulletin. Please contact Promega Technical Services if you have questions on useofthissystem.E-mail:********************Figure 1. Flow diagram showing preparation and use of CellTiter-Glo ®Reagent.Promega Corporation ·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·3170M A 12_0ACellTiter-Glo CellTiter-Glo MixerLuminometer®System Advantages•Homogeneous:“Add-mix-measure” format reduces the number of plate-handling steps to fewer than that required for similar ATP assays.•Fast:Data can be recorded 10 minutes after adding reagent.•Sensitive:Measures cells at numbers below the detection limits of standard colorimetric and fluorometric assays.•Flexible:Can be used with various multiwell formats. Data can be recorded by luminometer or CCD camera or imaging device.•Robust:Luminescent signal is very stable, with a half-life >5 hours,depending on cell type and culture medium used.•Able to Multiplex:Can be used with reporter gene assays or other cell-based assays from Promega (2,3).Figure 3. The luciferase reaction.Mono-oxygenation of luciferin is catalyzed byluciferase in the presence of Mg 2+, ATP and molecular oxygen.Promega Corporation ·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·3171M A 12_0A L u m i n e s c e n c e (R L U )Cells per Well10,00060,00020,00030,00040,00050,0000R² = 0.9990.5 × 1061.0 × 1061.5 × 1062.0 × 1062.5 × 1063.0 × 1063.5 × 1064.0 × 106r² = 0.99020,00010,00030,00040,00050,000r² = 0.9900100200300400HO SN S N O S N S N OCOOH +ATP+O 2Ultra-Glo™ Recombinant Luciferase +AMP+PP i +CO 2+LightBeetle Luciferin OxyluciferinMg 2+0Figure 2. Cell number correlates with luminescent output.A direct relationship exists between luminescence measured with the CellTiter-Glo ®Assay and the number of cells in culture over three orders of magnitude. Serial twofold dilutions of HEK293cells were made in a 96-well plate in DMEM with 10% FBS, and assays wereperformed as described in Section 3.B. Luminescence was recorded 10minutes after reagent addition using a GloMax ®-Multi+ Detection System. Values represent the mean ± S.D. of four replicates for each cell number. The luminescent signal from 50HEK293 cells is greater than three times the background signal from serum-supplemented medium without cells. There is a linear relationship (r 2= 0.99)between the luminescent signal and the number of cells from 0to 50,000 cells per well.Figure 4. Extended luminescent half-life allows high-throughput batchprocessing.Signal stability is shown for three common cell lines. HepG2 and BHK-21cells were grown and assayed in MEM containing 10% FBS, while CHO-K1 cells were grown and assayed in DME/F-12 containing 10% FBS. CHO-K1, BHK-21 and HepG2 cells, at 25,000 cells per well, were added to a 96-well plate. After an equal volume of CellTiter-Glo ®Reagent was added, plates were shaken and luminescence monitored over time with the plates held at 22°C. The half-lives of the luminescent signals for the CHO-K1, BHK-21 and HepG2 cells were approximately 5.4, 5.2 and5.8hours, respectively.2.Product Components and Storage ConditionsProduct Size Cat.#CellTiter-Glo ®Luminescent Cell Viability Assay 10ml G7570Substrate is sufficient for 100 assays at 100µl/assay in 96-well plates or 400 assays at 25µl/assay in 384-well plates. Includes:• 1 × 10mlCellTiter-Glo ®Buffer • 1 vial CellTiter-Glo ®Substrate (lyophilized)Product Size Cat.#CellTiter-Glo ®Luminescent Cell Viability Assay 10 × 10ml G7571Each vial of substrate is sufficient for 100 assays at 100µl/assay in 96-well plates or 400 assays at 25µl/assay in 384-well plates (1,000 to 4,000 total assays). Includes:•10 × 10mlCellTiter-Glo ®Buffer •10 vials CellTiter-Glo ®Substrate (lyophilized)Promega Corporation ·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·R e l a t i v e L u m i n e s c e n c e (%)Time (minutes)CHO-K101020304050607080901003173M A 12_0AProduct Size Cat.# CellTiter-Glo®Luminescent Cell Viability Assay100ml G7572 Substrate is sufficient for 1,000 assays at 100µl/assay in 96-well plates or 4,000assays at 25µl/assay in 384-well plates. Includes:•1 × 100ml CellTiter-Glo®Buffer• 1 vial CellTiter-Glo®Substrate (lyophilized)Product Size Cat.# CellTiter-Glo®Luminescent Cell Viability Assay10 × 100ml G7573Each vial of substrate is sufficient for 1,000 assays at 100µl/assay in 96-well plates or4,000 assays at 25µl/assay in 384-well plates (10,000to 40,000 total assays). Includes:•10 × 100ml CellTiter-Glo®Buffer•10 vials CellTiter-Glo®Substrate (lyophilized)Storage Conditions:For long-term storage, store the lyophilized CellTiter-Glo®Substrate and CellTiter-Glo®Buffer at –20°C. For frequent use, the CellTiter-Glo®Buffer can be stored at 4°C or room temperature for 48hours without loss of activity. See product label for expiration date information. ReconstitutedCellTiter-Glo®Reagent (Buffer plus Substrate) can be stored at room temperaturefor up to 8hours with <10% loss of activity, at 4°C for 48hours with ~5% lossof activity, at 4°C for 4days with ~20% loss of activity or at –20°C for 21weekswith ~3% loss of activity. The reagent is stable for up to ten freeze-thaw cycles,with less than 10% loss of activity.3.Performing the CellTiter-Glo®AssayMaterials to Be Supplied by the User•opaque-walled multiwell plates adequate for cell culture•multichannel pipette or automated pipetting station for reagent delivery•device (plate shaker) for mixing multiwell plates•luminometer, CCD camera or imaging device capable of reading multiwell plates •optional:ATP for use in generating a standard curve (Section 3.C)3.A.Reagent Preparation1.Thaw the CellTiter-Glo®Buffer, and equilibrate to room temperature priorto use. For convenience the CellTiter-Glo®Buffer may be thawed andstored at room temperature for up to 48hours prior to use.2.Equilibrate the lyophilized CellTiter-Glo®Substrate to room temperatureprior to use.Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·3.A.Reagent Preparation (continued)3.Transfer the appropriate volume (10ml for Cat.# G7570 and G7571, or 100mlfor Cat.# G7572 and G7573) of CellTiter-Glo ®Buffer into the amber bottlecontaining CellTiter-Glo ®Substrate to reconstitute the lyophilizedenzyme/substrate mixture. This forms the CellTiter-Glo ®Reagent.4.Mix by gently vortexing, swirling or inverting the contents to obtain ahomogeneous solution. The CellTiter-Glo ®Substrate should go intosolution easily in less than 1minute.3.B.Protocol for the Cell Viability AssayWe recommend that you perform a titration of your particular cells todetermine the optimal number and ensure that you are working within thelinear range of the CellTiter-Glo ®Assay. Figure 2 provides an example of sucha titration of HEK293 cells using 0 to 50,000 cells per well in a 96-well format.1.Prepare opaque-walled multiwell plates with mammalian cells in culturemedium, 100µl per well for 96-well plates or 25µl per well for 384-wellplates.Multiwell plates must be compatible with the luminometer used.2.Prepare control wells containing medium without cells to obtain a value forbackground luminescence.3.Add the test compound to experimental wells, and incubate according toculture protocol.4.Equilibrate the plate and its contents at room temperature forapproximately 30 minutes.5.Add a volume of CellTiter-Glo ®Reagent equal to the volume of cell culturemedium present in each well (e.g., add 100µl of reagent to 100µl of mediumcontaining cells for a 96-well plate, or add 25µl of reagent to 25µl ofmedium containing cells for a 384-well plate).6.Mix contents for 2 minutes on an orbital shaker to induce cell lysis.7.Allow the plate to incubate at room temperature for 10 minutes to stabilizeluminescent signal.Note:Uneven luminescent signal within standard plates can be caused bytemperature gradients, uneven seeding of cells or edge effects in multiwellplates.8.Record luminescence.Note:Instrument settings depend on the manufacturer. An integration timeof 0.25–1 second per well should serve as a guideline.Promega Corporation ·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·3.C.Protocol for Generating an ATP Standard Curve (optional)It is a good practice to generate a standard curve using the same plate onwhich samples are assayed. We recommend ATP disodium salt (Cat.# P1132,Sigma Cat.# A7699 or GE Healthcare Cat.# 27-1006). The ATP standard curveshould be generated immediately prior to adding the CellTiter-Glo®Reagentbecause endogenous ATPase enzymes found in sera may reduce ATP levels.1.Prepare 1µM ATP in culture medium (100µl of 1µM ATP solution contains10–10moles ATP).2.Prepare serial tenfold dilutions of ATP in culture medium (1µM to 10nM;100µl contains 10–10to 10–12moles of ATP).3.Prepare a multiwell plate with varying concentrations of ATP standard in100µl medium (25µl for a 384-well plate).4.Add a volume of CellTiter-Glo®Reagent equal to the volume of ATPstandard present in each well.5.Mix contents for 2 minutes on an orbital shaker.6.Allow the plate to incubate at room temperature for 10 minutes to stabilizethe luminescent signal.7.Record luminescence.4.Appendix4.A.Overview of the CellTiter-Glo®AssayThe assay system uses the properties of a proprietary thermostable luciferase toenable reaction conditions that generate a stable “glow-type” luminescentsignal while simultaneously inhibiting endogenous enzymes released duringcell lysis (e.g., ATPases). Release of ATPases will interfere with accurate ATPmeasurement. Historically, firefly luciferase purified from Photinus pyralis(LucPpy) has been used in reagents for ATP assays (1,4–7). However, it hasonly moderate stability in vitro and is sensitive to its chemical environment,including factors such as pH and detergents, limiting its usefulness fordeveloping a robust homogeneous ATP assay. Promega has successfullydeveloped a stable form of luciferase based on the gene from another firefly,Photuris pennsylvanica(LucPpe2), using an approach to select characteristics thatimprove performance in ATP assays. The unique characteristics of this mutant(LucPpe2m) enabled design of a homogeneous single-reagent-addition approachto perform ATP assays with cultured cells. Properties of the CellTiter-Glo®Reagent overcome the problems caused by factors, such as ATPases, thatinterfere with ATP measurement in cell extracts. The reagent is physicallyrobust and provides a sensitive and stable luminescent output.Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·4.A.Overview of the CellTiter-Glo®Assay (continued)Sensitivity and Linearity:The ATP-based detection of cells is more sensitivethan other methods (8–10). In experiments performed by Promega scientists,the luminescent signal from 50HEK293 cells is greater than three standarddeviations above the background signal from serum-supplemented mediumwithout cells. There is a linear relationship (r2= 0.99) between the luminescentsignal and the number of cells from 0 to 50,000 cells per well in the 96-wellformat. The luminescence values in Figure 2 were recorded after 10minutes ofincubation at room temperature to stabilize the luminescent signal as describedin Section3.B. Incubation of the same 96-well plate used in the experimentshown in Figure 2 for 360minutes at room temperature had little effect on therelationship between luminescent signal and number of cells (r2= 0.99).Speed:The homogeneous procedure to measure ATP using the CellTiter-Glo®Assay is quicker than other ATP assay methods that require multiple steps toextract ATP and measure luminescence. The CellTiter-Glo®Assay also is fasterthan other commonly used methods to measure the number of viable cells(such as MTT, alamarBlue®or Calcein-AM) that require prolonged incubationsteps to enable the cells’ metabolic machinery to convert indicator moleculesinto a detectable signal.4.B.Additional ConsiderationsTemperature:The intensity and decay rate of the luminescent signal from theCellTiter-Glo®Assay depends on the luciferase reaction rate. Environmentalfactors that affect the luciferase reaction rate will change the intensity andstability of the luminescent signal. Temperature is one factor that affects therate of this enzymatic assay and thus the light output. For consistent results,equilibrate assay plates to a constant temperature before performing the assay.Transferring eukaryotic cells from 37°C to room temperature has little effect onATP content (5). We have demonstrated that removing cultured cells from a37°C incubator and allowing them to equilibrate to 22°C for 1–2 hours hadlittle effect on ATP content. For batch-mode processing of multiple assayplates, take precautions to ensure complete temperature equilibration. Platesremoved from a 37°C incubator and placed in tall stacks at room temperaturewill require longer equilibration than plates arranged in a single layer.Insufficient equilibration may result in a temperature gradient effect betweenwells in the center and at the edge of the plates. The temperature gradientpattern also may depend on the position of the plate in the stack.Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·Chemicals:The chemical environment of the luciferase reaction affects theenzymatic rate and thus luminescence intensity. Differences in luminescenceintensity have been observed using different types of culture media and sera.The presence of phenol red in culture medium should have little impact onluminescence output. Assaying 0.1µM ATP in RPMI medium without phenolred resulted in ~5% increase in luminescence output (in relative light units[RLU]) compared to assays in RPMI containing the standard concentration ofphenol red, whereas assays in RPMI medium containing twice the normalconcentration of phenol red showed a ~2% decrease in luminescence.Solvents for the various test compounds may interfere with the luciferasereaction and thus the light output from the assay. Interference with theluciferase reaction can be detected by assaying a parallel set of control wellscontaining medium without cells. Dimethylsulfoxide (DMSO), commonly usedas a vehicle to solubilize organic chemicals, has been tested at finalconcentrations of up to 2% in the assay and only minimally affects light output.Plate Recommendations:We recommend using standard opaque-walledmultiwell plates suitable for luminescence measurements. Opaque-walledplates with clear bottoms to allow microscopic visualization of cells also maybe used; however, these plates will have diminished signal intensity andgreater cross talk between wells. Opaque white tape may be used to decreaseluminescence loss and cross talk.Cellular ATP Content:Different cell types have different amounts of ATP,and values reported for the ATP level in cells vary considerably (1,4,11–13).Factors that affect the ATP content of cells may affect the relationship betweencell number and luminescence. Anchorage-dependent cells that undergocontact inhibition at high densities may show a change in ATP content per cellat high densities, resulting in a nonlinear relationship between cell numberand luminescence. Factors that affect the cytoplasmic volume or physiology ofcells also will affect ATP content. For example, oxygen depletion is one factorknown to cause a rapid decrease in ATP (1).Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·4.B.Additional Considerations (continued)Mixing:Optimal assay performance is achieved when the CellTiter-Glo®Reagent is mixed completely with the cultured cells. Suspension cell lines (e.g., Jurkat cells) generally require less mixing to achieve lysis and extract ATP than adherent cells (e.g., L929 cells). Tests were done to evaluate the effect ofshaking the plate after adding the CellTiter-Glo® Reagent. Suspension cellscultured in multiwell plates showed only minor differences in light outputwhether or not the plates were shaken after adding the CellTiter-Glo®Reagent.Adherent cells are more difficult to lyse and show a substantial differencebetween shaken and nonshaken plates.Several additional parameters related to reagent mixing include the force ofdelivery of CellTiter-Glo®Reagent, sample volume and dimensions of the well.All of these factors may affect assay performance. The degree of reagent mixing required may be affected by the method used to add the CellTiter-Glo®Reagent to the assay plates. Automated pipetting devices using a greater or lesser force of fluid delivery may affect the degree of subsequent mixing required.Complete reagent mixing in 96-well plates should be achieved using orbitalplate shaking devices built into many luminometers and the recommended2-minute shaking time. Special electromagnetic shaking devices that use aradius smaller than the well diameter may be required to efficiently mixcontents of 384-well plates. The depth of medium and geometry of themultiwell plates may have an effect on mixing efficiency. We recommend that you take these factors into consideration when performing the assay andempirically determine whether a mixing step is necessary for the individualapplication.LuminometersFor highly sensitive luminometric assays, the luminometer model and settings greatly affect the quality of data obtained. Luminometers from differentmanufacturers will vary in sensitivities and dynamic ranges. We recommend the GloMax®products because these instruments do not require gainadjustments to achieve optimal sensitivity and dynamic range. Additionally, GloMax®instruments are preloaded with Promega protocols for ease of use.If you are not using a GloMax®luminometer, consult the operating manual for your luminometer to determine the optimal settings. The limits should beverified on each instrument before analysis of experimental samples. The assay should be linear in some portion of the detection range of the instrument used.For an individual luminometer there may be different gain settings. Werecommend that you optimize the gain settings.4.C.References1.Crouch, S.P. et al.(1993) The use of ATP bioluminescence as a measure of cellproliferation and cytotoxicity. J. Immunol. Methods160, 81–8.2.Farfan, A.et al.(2004) Multiplexing homogeneous cell-based assays. Cell Notes10, 2–5.3.Riss, T., Moravec, R. and Niles, A. (2005) Selecting cell-based assays for drugdiscovery screening. Cell Notes13, 16–21.4.Kangas, L., Grönroos, M. and Nieminen, A.L. (1984) Bioluminescence of cellular ATP:A new method for evaluating cytotoxic agents in vitro. Med. Biol.62, 338–43.5.Lundin, A. et al.(1986) Estimation of biomass in growing cell lines by adenosinetriphosphate assay.Methods Enzymol. 133, 27–42.6.Sevin, B.U. et al.(1988) Application of an ATP-bioluminescence assay in human tumorchemosensitivity testing. Gynecol. Oncol.31, 191–204.7.Gerhardt, R.T.et al.(1991) Characterization of in vitro chemosensitivity ofperioperative human ovarian malignancies by adenosine triphosphatechemosensitivity assay. Am. J. Obstet. Gynecol. 165, 245–55.8.Petty, R.D. et al.(1995) Comparison of MTT and ATP-based assays for themeasurement of viable cell number. J. Biolumin. Chemilumin.10, 29–34.9.Cree, I.A. et al.(1995) Methotrexate chemosensitivity by ATP luminescence in humanleukemia cell lines and in breast cancer primary cultures: Comparison of the TCA-100assay with a clonogenic assay. AntiCancer Drugs6, 398–404.10.Maehara, Y. et al.(1987) The ATP assay is more sensitive than the succinatedehydrogenase inhibition test for predicting cell viability. Eur. J. Cancer Clin. Oncol.23, 273–6.11.Stanley, P.E. (1986) Extraction of adenosine triphosphate from microbial and somaticcells. Methods Enzymol.133, 14–22.12.Beckers, B. et al.(1986) Application of intracellular ATP determination in lymphocytesfor HLA-typing. J. Biolumin. Chemilumin.1, 47–51.13.Andreotti, P.E. et al.(1995) Chemosensitivity testing of human tumors using amicroplate adenosine triphosphate luminescence assay: Clinical correlation forcisplatin resistance of ovarian carcinoma. Cancer Res. 55, 5276–82.4.D.Related ProductsCell Proliferation ProductsProduct Size Cat.# ApoLive-Glo™ Multiplex Assay10ml G6410 ApoTox-Glo™ Triplex Assay10ml G6320 CellTiter-Fluor™ Cell Viability Assay (fluorescent)10ml G6080 CellTiter-Blue®Cell Viability Assay (resazurin)20ml G8080 CellTiter 96®AQ ueous One SolutionCell Proliferation Assay (MTS, colorimetric)200 assays G3582 CellTiter 96®AQ ueous Non-RadioactiveCell Proliferation Assay (MTS, colorimetric)1,000 assays G5421 CellTiter 96®AQ ueous MTS Reagent Powder1g G1111 CellTiter 96®Non-RadioactiveCell Proliferation Assay (MTT, colorimetric)1,000 assays G4000 Additional sizes available.Cytotoxicity AssaysProduct Size Cat.# CytoTox-Glo™ Cytotoxicity Assay (luminescent)*10ml G9290Mitochondrial ToxGlo™ Assay*10ml G8000 MultiTox-Glo Multiplex Cytotoxicity Assay(luminescent, fluorescent)*10ml G9270 MultiTox-Fluor Multiplex Cytotoxicity Assay(fluorescent)*10ml G9200 CytoTox-Fluor™ Cytotoxicity Assay (fluorescent)*10ml G9260 CytoTox-ONE™ Homogeneous MembraneIntegrity Assay (LDH, fluorometric)*200–800 assays G7890 CytoTox-ONE™ Homogeneous MembraneIntegrity Assay, HTP1,000–4,000 assays G7892 CytoTox 96® Non-Radioactive Cytotoxicity Assay1,000 assays G1780 (LDH, colorimetric)*GSH-Glo™ Glutathione Assay10ml V691150ml V6912 GSH/GSSG-Glo™ Assay10ml V661150ml V6612 *Additional sizes available.LuminometersProduct Size Cat.# GloMax®-Multi+ Detection System with Instinct™ Software:Base Instrument with Shaking 1 each E8032 GloMax®-Multi+ Detection System with Instinct™ Software:Base Instrument with Heating and Shaking 1 each E9032 GloMax®-Multi+ Luminescence Module 1 each E8041Apoptosis ProductsProduct Size Cat.# Caspase-Glo®2 Assay*10ml G0940 Caspase-Glo®6 Assay*10ml G0970 Caspase-Glo®3/7 Assay* 2.5ml G8090 Caspase-Glo®8 Assay* 2.5ml G8200 Caspase-Glo®9 Assay* 2.5ml G8210Apo-ONE®Homogeneous Caspase-3/7 Assay1ml G7792 DeadEnd™ Fluorometric TUNEL System60 reactions G3250 DeadEnd™ Colorimetric TUNEL System20 reactions G7360Anti-ACTIVE®Caspase-3 pAb50µl G7481Anti-PARP p85 Fragment pAb50µl G7341Anti-pS473Akt pAb40µl G7441 Caspase Inhibitor Z-VAD-FMK, 20mM50µl G7231125µl G7232*Additional sizes available.(a)U.S. Pat. Nos. 6,602,677 and 7,241,584, European Pat. No. 1131441, Japanese Pat. Nos. 4537573 and 4520084 and other patents pending(b)U.S. Pat. No. 7,741,067, Japanese Pat. No. 4485470 and other patents pending.(c)U.S. Pat. No. 7,700,310, European Pat. No. 1546374 and other patents pending.(d)U.S. Pat. Nos 7,083,911, 7,452,663 and 7,732,128, European Pat. No. 1383914 and Japanese Pat. Nos. 4125600 and 4275715.(e)The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.© 2001–2012 Promega Corporation. All Rights Reserved.Anti-ACTIVE, Apo-ONE, Caspase-Glo, CellTiter 96, CellTiter-Blue, CellTiter-Glo, CytoTox 96 and GloMax are registered trademarks of Promega Corporation. ApoTox-Glo, ApoLive-Glo, CellTiter-Fluor, CytoTox-Fluor, CytoTox-Glo, CytoTox-ONE, DeadEnd, GSH-Glo, GSH/GSSG-Glo, Instinct, Mitochondrial ToxGlo and Ultra-Glo are trademarks of Promega Corporation. alamarBlue is a registered trademark of Trek Diagnostic Ssystems, Inc.Products may be covered by pending or issued patents or may have certain limitations. Please visit our Web site for more information.All prices and specifications are subject to change without prior notice.Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the most up-to-date information on Promega products.。

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Is there process in place to insure Review control plan /log proper control of sheets. sealant lots? Do gages used to monitor/control the All gauging equipment used to process have a range monitor and control the of measurement (gage process shall have a discrimination) to measurement range and cover the operating resolution to cover the range of the operating range of the equipment, and is the equipment. gage resolution sufficient? Review Process Control Plan How is sealant and Identify necessary steps contained for next to control part to next operation? operation.
GM Process Audit
Process Audit: Impregnation of Aluminum Castings Version: 1.0 Issue Date: October 17, 2011
Auditor: Supplier: Duns: Date: Location: Part Name:
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Review control plan /log sheets. Check specifically for Time and pressure requirements for process.
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Review control plan /log sheets.
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Equipment shall have drain cycle to avoid carry-over sealant to cold wash tank. Check for Maintenance records Review control plan /log sheets.
Change History
Question Number
Question
Requirements and Guidance
Objective Evidence
Assessment Not N/A Satisfactory Satisfactory Needs Immediate Action
Aluminum Castings Section of Audit
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Review control plan /log sheets.
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Leak testing machine should provide data ( Pressure ) for leakage for approval based on settings after Impregnation. Should have a dunk tester, too
Question Number
Question
Requirements and Guidance
Objective Evidence
Assessment Not N/A Satisfactory Satisfactory Needs Immediate Action
Audit Owner: Surendra Wankhede
Is the equipment Equipment shall have the used to clean capability to properly clean castings properly castings. ( Residual oils and maintained? surface rust / contamination) Look for maintenance records. Is the incoming material for cleaning Incoming material monitored reviewed for proper for proper certification. material requirements? Ask to see documentation Does the equipment Equipment shall be equipped properly prepare the such that Aluminum component casting for is properly prepared for Impregnation? Impregnation . Inspect a part in cue Leak testing machine should Does the leak testing provide data ( Pressure ) for machine checks leak? leakage based on settings before Impregnation. Watch while they test a part Are all process monitoring equipment All process monitoring and/or gages equipment and gages shall be identified as to identified as to their their calibration calibration status and be status and are the current. records current? Do gages used to monitor/control the All gauging equipment used to process have a range monitor and control the of measurement (gage process shall have a discrimination) to measurement range and cover the operating resolution to cover the range of the operating range of the equipment, and is the equipment. gage resolution sufficient? Review plant cleanliness and Is work area clean housekeeping to ensure the and free of outside work environment is free of elements? contamination.(see Contamination Process Audit) Be sure to examine dunnage. If sealant required Review Process Control Plan for Impregnation are and Identify all the adequate control manufacturing steps.(Including maintained? cycle time, pressure, etc.)Do they have control or run charts? Review Process Control Plan How are castings and Identify necessary steps contained for next to control part to next operation? operation. Read a few tags on skids
Auditor: Supplier: Duns: Date: Location: Part Name:
Байду номын сангаас
Question Number
Question
Requirements and Guidance
Objective Evidence
Assessment Not N/A Satisfactory Satisfactory Needs Immediate Action
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Sealant Section of Audit
What is the procedure to verify Sealant is correct and meets compositional requirements? What are the process monitoring checks used to maintain proper sealant requirements? Review customer specifications.
GM Process Audit
Process Audit: Impregnation of Aluminum Castings Version: 1.0 Issue Date: October 17, 2011
Auditor: Supplier: Duns: Date: Location: Part Name:
11
Review control plan /log sheets. Look for records of checks
12
GM Process Audit
Process Audit: Impregnation of Aluminum Castings Version: 1.0 Issue Date: October 17, 2011
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